Bioactive peptides from beef products fermented by acid whey in vitro and in silico study

This research investigated the potential of beef products with acid whey to release bioactive peptides and thereby emphasize their health-promoting potential. Peptide sequences were isolated and identified by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Firstly, the antihealth properties (toxicity, allergenicity) of the peptides were estimated based on the peptide sequences. Next, their health-promoting potential was demonstrated based on an in silico analysis by determining their bioactivity scores (PeptideRanker). Their various biological actions were also determined using BIOPEP-UWM tools. We presented peptide sequences with properties relevant to ensuring good health and well-being, including cardiovascular system, nervous and immune systems, or their support for the maintenance of general homeostasis. We obtained information on generation of biologically active peptides in uncured beef with acid whey and it can be considered as a new knowledge as it contributes to science development of functional and nutraceutical foods. In the long term, this information can be used in designing products with desired nutritional and health-promoting properties that are important for the well-being and for preventing the occurrence of noncommunicable diseases.

Bioactive peptides from beef products fermented by acid whey in vitro and in silico study

This research investigated the potential of beef products with acid whey to release bioactive peptides and thereby emphasize their health-promoting potential. Peptide sequences were isolated and identified by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Firstly, the antihealth properties (toxicity, allergenicity) of the peptides were estimated based on the peptide sequences. Next, their health-promoting potential was demonstrated based on an in silico analysis by determining their bioactivity scores (PeptideRanker). Their various biological actions were also determined using BIOPEP-UWM tools. We presented peptide sequences with properties relevant to ensuring good health and well-being, including cardiovascular system, nervous and immune systems, or their support for the maintenance of general homeostasis. We obtained information on generation of biologically active peptides in uncured beef with acid whey and it can be considered as a new knowledge as it contributes to science development of functional and nutraceutical foods. In the long term, this information can be used in designing products with desired nutritional and health-promoting properties that are important for the well-being and for preventing the occurrence of noncommunicable diseases.(AU)

Seletividade de sulfentrazone na cultura do girassol / Selectivity of sulfentrazone in sunflower crop

Objetivou-se avaliar a seletividade da molécula sulfentrazone em pós-emergência na cultura do girassol. O experimento foi conduzido no ano de 2014 em Campo Novo do Parecis. O delineamento foi em blocos casualizados com nove tratamentos e quatro repetições. Os tratamentos foram constituídos de sete doses de sulfentrazone (50, 62,5, 75, 87,5, 100, 112,5 e 125 g i.a ha-1) e uma testemunha com capina manual. A aplicação do herbicida foi realizada com pulverizador costal pressurizado a CO2 e equipado com barra de aplicação contendo cinco pontas do modelo TeeJet XR 110 02VS, com volume de aplicação de 200 L ha-1. Foi avaliada a fitotoxicidade (%), altura do capítulo, diâmetro da haste, massa do capítulo, massa de aquênios por capítulo, produtividade e massa de mil aquênios. Houve redução na altura do capítulo nas doses de 112,5 e 125g i.a ha-1, enquanto que nas demais variáveis não foram observadas diferenças significativas. Até os 14 dias após a aplicação (DAA) foram observadas algumas injúrias. A produtividade não foi afetada pelas doses de sulfentrazone. Que se mostraram seletivas à cultura do girassol.

The objective of this study was to evaluate the selectivity of the post emergent sulfentrazone molecule in sunflower crop. The experiment was conducted in 2014 in Campo Novo do Parecis. The design was in randomized blocks with nine treatments and four replications. Treatments consisted of seven doses of sulfentrazone (50, 62.5, 75, 87.5, 100, 112.5 and 125 g i.a ha-1) and a control with weeding. The herbicide application was carried out with a CO2pressurized costal sprayer and equipped with an application bar containing five TeeJet XR 110 02 VS tips, with an application volume of 200 L ha-1. Phytotoxicity (%), chapter height, stem diameter, chapter mass, achenes mass per chapter, yield and thousand achenes mass were evaluated. There was a reduction in chapter height at 112.5 and 125 g i.a ha-1, while no significant differences were observed in the other variables. Up to 14 days after application (DAA) some injuries were observed. Yield was not affected by sulfentrazone doses. Which proved selective to sunflower crop.

Seletividade de sulfentrazone na cultura do girassol / Selectivity of sulfentrazone in sunflower crop

Objetivou-se avaliar a seletividade da molécula sulfentrazone em pós-emergência na cultura do girassol. O experimento foi conduzido no ano de 2014 em Campo Novo do Parecis. O delineamento foi em blocos casualizados com nove tratamentos e quatro repetições. Os tratamentos foram constituídos de sete doses de sulfentrazone (50, 62,5, 75, 87,5, 100, 112,5 e 125 g i.a ha-1) e uma testemunha com capina manual. A aplicação do herbicida foi realizada com pulverizador costal pressurizado a CO2 e equipado com barra de aplicação contendo cinco pontas do modelo TeeJet XR 110 02VS, com volume de aplicação de 200 L ha-1. Foi avaliada a fitotoxicidade (%), altura do capítulo, diâmetro da haste, massa do capítulo, massa de aquênios por capítulo, produtividade e massa de mil aquênios. Houve redução na altura do capítulo nas doses de 112,5 e 125g i.a ha-1, enquanto que nas demais variáveis não foram observadas diferenças significativas. Até os 14 dias após a aplicação (DAA) foram observadas algumas injúrias. A produtividade não foi afetada pelas doses de sulfentrazone. Que se mostraram seletivas à cultura do girassol.(AU)

The objective of this study was to evaluate the selectivity of the post emergent sulfentrazone molecule in sunflower crop. The experiment was conducted in 2014 in Campo Novo do Parecis. The design was in randomized blocks with nine treatments and four replications. Treatments consisted of seven doses of sulfentrazone (50, 62.5, 75, 87.5, 100, 112.5 and 125 g i.a ha-1) and a control with weeding. The herbicide application was carried out with a CO2pressurized costal sprayer and equipped with an application bar containing five TeeJet XR 110 02 VS tips, with an application volume of 200 L ha-1. Phytotoxicity (%), chapter height, stem diameter, chapter mass, achenes mass per chapter, yield and thousand achenes mass were evaluated. There was a reduction in chapter height at 112.5 and 125 g i.a ha-1, while no significant differences were observed in the other variables. Up to 14 days after application (DAA) some injuries were observed. Yield was not affected by sulfentrazone doses. Which proved selective to sunflower crop.(AU)

Potentially toxic and medicinal plants from community assisted in UFPel Veterinary Clinic / Plantas com potencial medicinal e tóxico em comunidade atendida pelo Ambulatório Veterinário-UFPel

The aim of this study was to identify plants used in traditional medicine, as well as those with toxic potential in the neighborhoods of Simões Lopes, Padre Réus and Porto in the city of Pelotas. For a year there were interviewed 111 owners of animals treated at the Veterinary Clinic-Universidade Federal de Pelotas, in order to assess the knowledge, cultivation, use and form of obtaining medicinal plants, age and sex of people, number of animals and results of the use of plants in the animals. After initial investigation, visits were held in the community aiming to shoot and collect samples of plants for botanical identification. They were listed 74 plants for medicinal purposes, mainly from Asteraceae and Lamiaceae families, minimally used in animals. The main indications of use were for digestive and integumentary system, followed by musculoskeletal and respiratory, by infusion and decoction. Most of the interviewers were unaware of the adverse effects of inappropriate use of medicinal plants, and different species were known with the same common name, so they could result in toxicity, wrong dosages and unknown actions. There was a predominance of cultivation and use of medicinal plants in people with an average age of 41.2 years who obtained knowledge mainly from parents and grandparents. The toxic potential plants found were from Araceae, Euphorbiaceae and Araliaceae families, in a total of 20 species, but only six were cited as toxic by the interviewees. There was noticed the existence and use of many medicinal plants and presence of potentially toxic species in the region; so it is essential to evidence scientifically possible benefits and risks of administration in animals.(AU)

O objetivo deste trabalho foi identificar plantas utilizadas como medicinais e aquelas com potencial tóxico localizadas no bairro Simões Lopes, Padre Réus e Porto em Pelotas-RS. Durante um ano foram entrevistados 111 proprietários de animais atendidos no Ambulatório Veterinário-Universidade Federal de Pelotas, com o propósito de avaliar o conhecimento, cultivo, utilização e forma de obtenção das plantas medicinais, faixa etária e o sexo das pessoas, número de animais nas residências e resultados do uso de plantas nos animais. Após investigação inicial, realizaram-se visitas na comunidade com intuito de fotografar e coletar amostras das plantas para identificação botânica. Foram listadas 74 plantas com fins medicinais, principalmente das famílias Asteraceae e Lamiaceae, usadas em minoria nos animais. As principais indicações de uso foram para o sistema digestório e tegumentar, seguido do musculoesquelético e respiratório, sob forma de infusão e decocto. Grande parte dos entrevistados desconhecia os efeitos adversos do uso inadequado de plantas medicinais, e espécies diferentes eram conhecidas com o mesmo nome popular, podendo resultar em toxicidade, dosagens errôneas e ações desconhecidas. Em relação ao cultivo e utilização das plantas medicinais, predominava em pessoas com idade média de 41,2 anos, que obtiveram o conhecimento principalmente de pais e avós. As plantas com potencial tóxico encontradas pertenciam às famílias Araceae, Euphorbiaceae e Araliaceae, totalizando 20 espécies; porém, apenas seis foram citadas como tóxicas pelos entrevistados. Assim, observou-se a existência e uso de muitas plantas medicinais, além da presença de espécies potencialmente tóxicas na região, sendo imprescindível ressaltar, com embasamento científico,possíveis benefícios e riscos da administração em animais.(AU)