Bilateral pyelonephritis due to Escherichia coli infection in a captive jaguar (Panthera onca) / Pielonefrite bilateral por Escherichia coli em uma onça-pintada (Panthera onca)

Extraintestinal pathogenic Escherichia coli (ExPEC) is a highly diverse pathotype of E. coli which colonizes the intestine, and it is considered an important etiological agent associated with bacteremia and other systemic infections, among them urinary tract infection. Retrospective studies evaluating morbidity and mortality of nondomestic felids have demonstrated that urinary tract diseases are among the main causes of death for geriatric animals. Also, mesenchymal neoplasms of the uterus are common in wild felids, and they possess variable morphologic characteristics related to invasiveness and malignancy. This report describes a case of bilateral pyelonephritis due to extraintestinal uropathogenic E. coli infection in a captive jaguar (Panthera onca). The diagnosis was confirmed through pathological, bacterial and immunohistochemical findings. According to molecular analysis, this E. coli strain was classified in the phylogroup F, possessing the following virulence-associated genes: usp, cnf-1, hlyA, papC and sfa. Additionally, this E. coli was highly resistant to beta-lactams and first-generation cephalosporin. This jaguar also presented a uterine leiomyoma with distinct distribution, and severe degenerative articular disease, both of them described as frequently seen lesions in geriatric animals from the Panthera genus.(AU)

Escherichia coli extraintestinal patogênica (ExPEC) é um patotipo altamente diverso de E. coli que coloniza o intestino e é considerada um agente etiológico importante, associado com bacteremia e outras infecções sistêmicas, dentre elas infecções do trato urinário. Estudos retrospectivos avaliando morbidade e mortalidade de felídeos não domésticos demostram que doenças do trato urinário estão entre as principais causas de morte de animais geriátricos. Ainda, neoplasias mesenquimais uterinas são comuns em felídeos de cativeiro e possuem características morfológicas variáveis relacionadas a invasividade e malignidade. Neste relato é descrito um caso de pielonefrite bilateral por E. coli extraintestinal uropatogênica em uma onça-pintada de cativeiro (Panthera onca). O diagnóstico foi confirmado através dos achados patológicos, bacteriológicos e imuno-histoquímicos. A partir da análise molecular, esta cepa de E. coli foi classificada no filogrupo F, possuindo os seguintes genes associados a virulência: usp, cnf-1, hlyA, papC and sfa. Adicionalmente, a bactéria isolada foi altamente resistente a ß-lactâmicos e cefalosporinas de primeira geração. Foi observado ainda um leiomioma uterino com distribuição distinta e doença articular degenerativa severa, ambas descritas na literatura como comumente observadas em animais geriátricos do gênero Panthera.(AU)

Uterine leiomyosarcoma with secondary acute hemoabdomen in a goat

Neoplasms of the reproductive tract are uncommonly reported in goats. Here we describe a case of sudden death caused by acute hemoabdomen due to a ruptured uterine leiomyosarcoma in a 7-year-old female mixed breed goat. Gross changes included oral and ocular pallor, as well as 3 liters of blood and blood clots in the abdominal cavity. The uterine body wall was irregularly thickened and firm, and had a 2 cm in diameter, transmural perforation. The uterine lumen was filled with dark red to brown blood clots admixed with friable necrotic material. Histologically, a poorly-demarcated neoplasm expanded and effaced the uterine wall. The neoplasm consisted of densely packed bundles and streams of elongate neoplastic cells with moderate pleomorphism and abundant eosinophilic cytoplasm and elongate nuclei with coarsely stippled chromatin. The mitotic count was 4 per 2.37 mm2. Multiple areas of necrosis and clusters of lymphocytes and plasma cells were present throughout the neoplasm. Neoplastic cells exhibited diffuse immunolabeling for smooth muscle actin and patchy immunolabeling for desmin, consistent with a uterine leiomyosarcoma.(AU)

Uterine leiomyosarcoma with secondary acute hemoabdomen in a goat

Neoplasms of the reproductive tract are uncommonly reported in goats. Here we describe a case of sudden death caused by acute hemoabdomen due to a ruptured uterine leiomyosarcoma in a 7-year-old female mixed breed goat. Gross changes included oral and ocular pallor, as well as 3 liters of blood and blood clots in the abdominal cavity. The uterine body wall was irregularly thickened and firm, and had a 2 cm in diameter, transmural perforation. The uterine lumen was filled with dark red to brown blood clots admixed with friable necrotic material. Histologically, a poorly-demarcated neoplasm expanded and effaced the uterine wall. The neoplasm consisted of densely packed bundles and streams of elongate neoplastic cells with moderate pleomorphism and abundant eosinophilic cytoplasm and elongate nuclei with coarsely stippled chromatin. The mitotic count was 4 per 2.37 mm2. Multiple areas of necrosis and clusters of lymphocytes and plasma cells were present throughout the neoplasm. Neoplastic cells exhibited diffuse immunolabeling for smooth muscle actin and patchy immunolabeling for desmin, consistent with a uterine leiomyosarcoma.

The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Masson’s trichrome and PAS stainings, respectively.[...]

The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Massons trichrome and PAS stainings, respectively.[...](AU)

The therapeutic effects of curcumin and capsaicin against cyclophosphamide side effects on the uterus in rats

Purpose: To evaluate the impact of systemic cyclophosphamide treatment on the rat uterus and investigate the potential therapeutic effects of natural antioxidant preparations curcumin and capsaicin against cyclophosphamide side effects. Methods: A 40 healthy adult female Wistar albino rats were used in this study. Rats were randomly divided into four groups to determine the effects of curcumin and capsaicin against Cyclophosphamide side effects on the uterus (n=10 in each group); Group 1 was the control group (sham-operated), Group 2 was the cyclophosphamide group, Group 3 was the cyclophosphamide + curcumin (100mg/kg) group, and Group 4 was the cyclophosphamide + capsaicin (0.5 mg/kg) group. Results: Increased tissue oxidative stress and histological damage in the rat uterus were demonstrated due to the treatment of systemic cyclophosphamide chemotherapy alone. The level of tissue oxidant and antioxidant markers and histopathological changes were improved by the treatment of curcumin and capsaicin. Conclusion: Cytotoxic effects of natural alkylating chemotherapeutic agents like cyclophosphamide on the uterus can be prevented by curcumin and capsaicin.(AU)

Estrogen and progesterone receptors immunolabeling in mammary solid carcinoma in an African Hedgehog (Atelerix albiventris) with concurrent uterine fibrosarcoma

The African Hedgehog (Atelerix albiventris) has gained popularity as a pet in several parts of the world. This is a report of a concomitant finding of spontaneous mammary and uterine tumors in an adult female African Hedgehog. The animal presented abdominal masses and died suddenly. Upon necropsy, not only the mammary tumor mass, but also a uterine neoplasm was found. Formalin fixed and paraffin embedded tissues were submitted for histopathological examination. The mammary tumor was diagnosed as a solid carcinoma, and the uterine tumor as fibrosarcoma. By immunohistochemistry, the mammary tumor showed positivity for pancytokeratins and estrogen and progesterone receptors, while the uterine neoplasm exhibited positivity for vimentin. There was high cross-immunoreactivity between anti-human antibodies. The positivity for hormonal receptors antigens may represent a relationship between estrogen and progesterone levels and the development of mammary tumors, as in other mammals.

Estrogen and progesterone receptors immunolabeling in mammary solid carcinoma in an African Hedgehog (Atelerix albiventris) with concurrent uterine fibrosarcoma

The African Hedgehog (Atelerix albiventris) has gained popularity as a pet in several parts of the world. This is a report of a concomitant finding of spontaneous mammary and uterine tumors in an adult female African Hedgehog. The animal presented abdominal masses and died suddenly. Upon necropsy, not only the mammary tumor mass, but also a uterine neoplasm was found. Formalin fixed and paraffin embedded tissues were submitted for histopathological examination. The mammary tumor was diagnosed as a solid carcinoma, and the uterine tumor as fibrosarcoma. By immunohistochemistry, the mammary tumor showed positivity for pancytokeratins and estrogen and progesterone receptors, while the uterine neoplasm exhibited positivity for vimentin. There was high cross-immunoreactivity between anti-human antibodies. The positivity for hormonal receptors antigens may represent a relationship between estrogen and progesterone levels and the development of mammary tumors, as in other mammals.(AU)

Tumor uterino em bovino relato de caso / Tumor - case report

As descrições de tumores uterinos em bovinos não são freqüentes, embora o câncer uterino não seja um achado raro. Este relato apresenta o caso de um bovino do sexo feminino, raça Holandesa, 11 anos de idade, escore de condição corporal 3,5, oito partos e produção leiteira de sete litros à época do atendimento, que foi atendido a campo em propriedade rural de atividade leiteira. À palpação retal, foi diagnosticada uma massa tumoral de consistência firme no corpo do útero, estendendo-se até um dos cornos. Ao exame ultrassonográfico, foi confirmada a presença de tumoração no corpo uterino com 15,3 cm de diâmetro. O tratamento cirúrgico foi descartado pelo proprietário, devido aos riscos cirúrgicos e dificuldade com os cuidados pós-operatórios a campo. Anestro e ciclos estrais curtos, com 10 a 15 dias de duração, foram os principais sinais clínicos observados. (AU)

Descriptions of uterine tumors in cattle are infrequent, although uterine cancer is not a rare finding. This report presents the case of a bovine female, Holstein, 11 years of age, body condition score 3.5, eight births and seven liters of milk production at the time of the service, which was attended in rural property of dairy activity. During transrectal palpation, was diagnosed a tumor with firm mass in the body of the uterus, extending to one of the horns. Ultrasound exam confirmed the presence of the tumor in the uterine body with 15.3 cm diameter. Surgical treatment was discarded by the owner due to surgical risks and difficulty with postsurgical care at field. Anestrus and short estrous cycles, with 10-15 days of duration, were the main clinical signs. (AU)

Tumor uterino em bovino – relato de caso / Tumor - case report

As descrições de tumores uterinos em bovinos não são freqüentes, embora o câncer uterino não seja um achado raro. Este relato apresenta o caso de um bovino do sexo feminino, raça Holandesa, 11 anos de idade, escore de condição corporal 3,5, oito partos e produção leiteira de sete litros à época do atendimento, que foi atendido a campo em propriedade rural de atividade leiteira. À palpação retal, foi diagnosticada uma massa tumoral de consistência firme no corpo do útero, estendendo-se até um dos cornos. Ao exame ultrassonográfico, foi confirmada a presença de tumoração no corpo uterino com 15,3 cm de diâmetro. O tratamento cirúrgico foi descartado pelo proprietário, devido aos riscos cirúrgicos e dificuldade com os cuidados pós-operatórios a campo. Anestro e ciclos estrais curtos, com 10 a 15 dias de duração, foram os principais sinais clínicos observados.

Descriptions of uterine tumors in cattle are infrequent, although uterine cancer is not a rare finding. This report presents the case of a bovine female, Holstein, 11 years of age, body condition score 3.5, eight births and seven liters of milk production at the time of the service, which was attended in rural property of dairy activity. During transrectal palpation, was diagnosed a tumor with firm mass in the body of the uterus, extending to one of the horns. Ultrasound exam confirmed the presence of the tumor in the uterine body with 15.3 cm diameter. Surgical treatment was discarded by the owner due to surgical risks and difficulty with postsurgical care at field. Anestrus and short estrous cycles, with 10-15 days of duration, were the main clinical signs.

Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...(AU)

Adenocarcinoma endometrial em uma coelha (Oryctolagus cuniculus) / Endometrial adenocarcinoma in a rabbit (Oryctolagus cuniculus)

Background: Uterine tumors are classified according to their origin, epithelial or mesenchymal. These neoplasms are uncommon in most animal species, but are considered as important causes of reproductive disorders. In rabbits, the occurrence of these tumors increase with development of the animals and, although the numbers indicate a high occurrence of this disease in rabbits, reports are scarce. Therefore, this paper has the objective to describe a case of endometrial adenocarcinoma in a rabbit, emphasizing their histological features as well as provide a brief discussion about the epidemiological, clinical, pathological and prevention forms and treatment. Case: A 4-years old New Zealand rabbit breed, from a group of animals maintained only for breeding, had low fertility. The rabbit was twice covered by proven fertile animals, but no pregnancy, becoming unfit reproduction and subjected to euthanasia. At necropsy, tissue samples were collected, subsequently fixed in 10% formalin and then were processed routinely and stained with hematoxylin and eosin for histopathological analysis. Grossly, there was swelling of the uterine body, characterized by two masses that protruded from the endometrium, with rounded and soft look, and the court had cystic cavity filled with whitish gelatinous consistency of content. Microscopic cancer cells that originated from the endometrial...

Ultra-sonografia das patologias uterinas em cadelas / Ultrasound of the canine uterine patologies

A ultra-sonografia de alta resolução, em tempo real, é um auxílio diagnóstico de grande importância para avaliação das diferentes patologias uterinas, pois permite a visualização do útero em suas dimensões, espessuras, tipos de parede, conteúdo do fluido intra-uterino e de possíveis formações (neoplasias) nele contidas. Utilizando-se equipamentos de alta resolução de imagem e transdutores de alta freqüência (acima de 10 MHz), torna-se possível à visualização de detalhes na parede uterina, assim como, identificar a presença de micro formações (cistos endometriais) e ecotextura do conteúdo luminal, tornando-se possível diagnósticoem diversas patologias que acometem o útero(AU)

Ultrasonography of the high resolution, in real time, is a diagnostic AID of great importance for evaluation of the different uterine pathology, therefore it allows the visualization of the uterus in its dimension, thicknesses types of wall, content of the intrauterine fluid and possible formations (neoplasias) in it contained. Using high resolution equipment of high-frequency image and transducers (above 10 MHz), becomes possible the visualization of details in the uterine wall, as well as, to identify to the micro presence formations (endometrial cysts) and e ecotexture of the luminal content, becoming possible the diagnostic aid in diverse pathology that(AU)

Ultra-sonografia das patologias uterinas em cadelas / Ultrasound of the canine uterine patologies

A ultra-sonografia de alta resolução, em tempo real, é um auxílio diagnóstico de grande importância para avaliação das diferentes patologias uterinas, pois permite a visualização do útero em suas dimensões, espessuras, tipos de parede, conteúdo do fluido intra-uterino e de possíveis formações (neoplasias) nele contidas. Utilizando-se equipamentos de alta resolução de imagem e transdutores de alta freqüência (acima de 10 MHz), torna-se possível à visualização de detalhes na parede uterina, assim como, identificar a presença de micro formações (cistos endometriais) e ecotextura do conteúdo luminal, tornando-se possível diagnósticoem diversas patologias que acometem o útero

Ultrasonography of the high resolution, in real time, is a diagnostic AID of great importance for evaluation of the different uterine pathology, therefore it allows the visualization of the uterus in its dimension, thicknesses types of wall, content of the intrauterine fluid and possible formations (neoplasias) in it contained. Using high resolution equipment of high-frequency image and transducers (above 10 MHz), becomes possible the visualization of details in the uterine wall, as well as, to identify to the micro presence formations (endometrial cysts) and e ecotexture of the luminal content, becoming possible the diagnostic aid in diverse pathology that