Amplified neurobehavioral and hormonal alterations following the combination of prenatal and postnatal stress in first-time rats

In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.(AU)

Amplified neurobehavioral and hormonal alterations following the combination of prenatal and postnatal stress in first-time rats

In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.

The behavior of Etawah Grade goats in early and late pregnancyperiod in a tropical area

The objective of the study was to identify the different behavior of Etawah Grade does in early and late pregnancy period. This research using 14 head of Etawah Grade does in pregnant conditions with an age of fewer than 2 months as the early pregnancy group and gestational age of 4 to 5 months as the late pregnancy group in Farm of FacultyAnimal Science, Universitas Gadjah Mada. The method usedwas direct observation. The goats were observed for 24 hours. The data observed were consisted of activities including time and frequency of feeding, ruminating, standing, lying, urinating and defecating. Data behavior of goats was analyzed using T-test. The results showed that the temperature and humidity of the study ranged from 25 to 31 ºC and 65 to 90%, respectively. The heart rate frequency in late pregnancy higher (P < 0.05) than in early pregnancy conditions. There were significant differences (P < 0.05) on the behavior of early and late pregnancy of Etawah Grade does, in terms of frequency of feeding (17.11±1.33 and 13.61±2.85 times/day), and average in once feeding (10.24±1.64 and 12.88±2.36 minutes), standing duration (15.05±0.83 and 11.45±1.34 hours/day), laying duration (9.01±0.71 and 12.54±1.34 hours/day), average in once laying (31.32±3.6 and 47.89±5.68), and frequency of urination (6.56±0.34 and 9.55±2.01). The conclusion of the study is the Etawah Grade does during early pregnancy and late pregnancy has different behavior, especially on the frequency of feeding, average on once feeding, standing duration, time and average of once lying, and frequency of urination.(AU)

The behavior of Etawah Grade goats in early and late pregnancyperiod in a tropical area

The objective of the study was to identify the different behavior of Etawah Grade does in early and late pregnancy period. This research using 14 head of Etawah Grade does in pregnant conditions with an age of fewer than 2 months as the early pregnancy group and gestational age of 4 to 5 months as the late pregnancy group in Farm of FacultyAnimal Science, Universitas Gadjah Mada. The method usedwas direct observation. The goats were observed for 24 hours. The data observed were consisted of activities including time and frequency of feeding, ruminating, standing, lying, urinating and defecating. Data behavior of goats was analyzed using T-test. The results showed that the temperature and humidity of the study ranged from 25 to 31 ºC and 65 to 90%, respectively. The heart rate frequency in late pregnancy higher (P < 0.05) than in early pregnancy conditions. There were significant differences (P < 0.05) on the behavior of early and late pregnancy of Etawah Grade does, in terms of frequency of feeding (17.11±1.33 and 13.61±2.85 times/day), and average in once feeding (10.24±1.64 and 12.88±2.36 minutes), standing duration (15.05±0.83 and 11.45±1.34 hours/day), laying duration (9.01±0.71 and 12.54±1.34 hours/day), average in once laying (31.32±3.6 and 47.89±5.68), and frequency of urination (6.56±0.34 and 9.55±2.01). The conclusion of the study is the Etawah Grade does during early pregnancy and late pregnancy has different behavior, especially on the frequency of feeding, average on once feeding, standing duration, time and average of once lying, and frequency of urination.