Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines / Uso do MALDI-TOF para identificação e monitoramento de bactérias Gram-negativas em psitacídeos cativos

Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.

Estudos microbiológicos da sanidade de psitacídeos que serão reintroduzidos são importantes para avaliar se esses animais atuam como portadores de agentes patogênicos para outros animais e humanos. A espectrometria de massa por ionização/dessorção de matriz assistida por laser/tempo de vôo (MALDI-TOF MS) é um método mais rápido e preciso para identificar bactérias na comparação com métodos convencionais de microbiologia. O objetivo deste estudo foi avaliar o estado de saúde de psitacídeos cativos, identificando bactérias Gram-negativas da microbiota fecal por MALDI -TOF MS. Os resultados indicaram alta frequência de bactérias Gram-negativas nas fezes (96,5%), principalmente da família Enterobacteriaceae (88,7%). As mais prevalentes foram Escherichia coli (39,0%), Proteus vulgaris (12,2%), Klebsiella spp. (12,1%) e Raoultella ornithinolytica (8,7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. e Escherichia hermannii foram isolados com menor frequência. Todos esses agentes são potencialmente patogênicos para os papagaios e podem causar infecções sistêmicas em outros animais e seres humanos. Esses achados reforçam que o MALDI- TOF MS é um método rápido e preciso de identificação do microrganismo e avaliação do estado de saúde dos psitacídeos, fornecendo dados relevantes para auxiliar na tomada de decisões sobre os protocolos sanitários em centros de triagem de animais selvagens e sobre a possibilidade de reintrodução futura.

Enterobactérias no leite comercializado informalmente em quatro municípios do sul do Piauí, Brasil / Enterobacteriaceae in raw milk informally marketed in four cities of South of the Piauí, Brazil

Enterobacteriaceae can be present in the environment and in the intestine of warmblooded animals. This study aimed to isolate and identify enterobacteria in informally marketed milk in cities of South of the Piaui. 52 milk samples were acquired at different points of Bom Jesus, Redenção do Gurguéia, Santa Luz and Cristino Castro, Piaui, which were taken into Styrofoam containing ice to food microbiology laboratory of the Federal University of Piauí, in Bom Jesus, where held the bacterial isolation in MacConkey, EMB and XLD. The conclusive biochemical identification and agglutination test was held at the Enterobacteria Laboratory of FIOCRUZ, (Rio de Janeiro-RJ). From the 37 strains, identified the species Escherichia coli, Enterobacter cloacae, Enterobacter gergoviae, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobater freundii, Providencia rettigeri and Serratia marcescens. The informally marketed milk is contaminated by Enterobacteriaceae that can be pathogenic and should be taken preventive measures, such as pasteurization of milk.

Enterobactérias no leite comercializado informalmente em quatro municípios do sul do Piauí, Brasil / Enterobacteriaceae in raw milk informally marketed in four cities of South of the Piauí, Brazil

Enterobacteriaceae can be present in the environment and in the intestine of warmblooded animals. This study aimed to isolate and identify enterobacteria in informally marketed milk in cities of South of the Piaui. 52 milk samples were acquired at different points of Bom Jesus, Redenção do Gurguéia, Santa Luz and Cristino Castro, Piaui, which were taken into Styrofoam containing ice to food microbiology laboratory of the Federal University of Piauí, in Bom Jesus, where held the bacterial isolation in MacConkey, EMB and XLD. The conclusive biochemical identification and agglutination test was held at the Enterobacteria Laboratory of FIOCRUZ, (Rio de Janeiro-RJ). From the 37 strains, identified the species Escherichia coli, Enterobacter cloacae, Enterobacter gergoviae, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobater freundii, Providencia rettigeri and Serratia marcescens. The informally marketed milk is contaminated by Enterobacteriaceae that can be pathogenic and should be taken preventive measures, such as pasteurization of milk.(AU)