Resumo
Purpose: To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation.(AU)
Objetivo: Quantificar fibroblastos de queloide apos irradiacao com LED azul de 470nm, in vitro. Metodos: Foram obtidos fibroblastos de queloide e pele adjacente, de seis pacientes. As celulas foram cultivadas e mantidas em meio de cultura DMEM. Em placas de Petri, receberam irradiacao com doses de energia de 6J, 12J e 18J. Apos 24 horas a contagem foi feita pela media da triplicata para cada amostra. Resultados: Nao houve diferenca na quantidade de fibroblastos de queloide irradiados nas doses estudadas (p=0,261). Observou-se diferenca nos fibroblastos de pele adjacente (p=0,025), com relacao as doses de 18 J e 6 J (p=0,03). Conclusoes: Houve reducao dos fibroblastos de pele adjacente irradiados com LED azul de 470 nm na dose de energia de 18 J em relacao a dose de 6 J. Nao houve alteracao na quantidade de fibroblastos de queloide nas doses aplicadas apos 24 horas da irradiacao.(AU)