Expressão gênica dos REα, REβ e PR em tumores mamários de cadelas por meio do q-PCR / REα, REβ and PR gene expression in mammary gland tumors of female dogs by q-PCR

A expressão de receptores de estrógeno (ER) e progesterona (PR) por meio da técnica de q-PCR foi avaliada em 26 cadelas portadoras de neoplasias mamárias e cinco cadelas sem afecções mamárias (grupo controle). Os resultados mostraram que os três grupos de animais estudados - com tumor maligno ou benigno e controle - expressaram receptores de estrógeno alfa, beta e progesterona. A quantificação relativa mostrou tendência para uma expressão maior de receptores no grupo controle e menor no grupo de animais com neoplasias malignas. Além disso, observou-se expressão maior de ERα em relação ao ERβ, e as neoplasias malignas de origem mista apresentaram maiores concentrações dos receptores PR, ERα e ERβ que as neoplasias de origem epitelial.(AU)

The estrogen and progesterone receptor (ER and PR) expression with the q-PCR technique was evaluated in 26 female dog carrying of mammary tumors and five female dogs without mammary disease (control group). The results showed that the three animal groups evaluated - malignant or benign tumor and control - expressed alpha and beta estrogen and progesterone receptors. The relative quantification showed a tendency for a higher expression of receptors from the control group and smaller in the malignat tumors animal group. Also, there was a major ERα expression regarding to ERβ and the malignat tumors from mixed origin presented higher concentrations of receptors PR, ERα and ERβ, when compared to tumors of epithelial origin.(AU)

Immunohistochemical study of estrogen and progesterone receptors and cell proliferative indexes in canine inflammatory mammary carcinoma: 9 cases

Inflammatory mammary carcinoma (IMC) is a unique form of mammary cancer that affects women and female dogs. Hallmarks of IMC include clinical signs of inflammed affected glands and invasion of dermal lymphatics by tumor cells. Due to locally aggressive behavior and high metastatic rate, prognosis is considered poor. No satisfactory treatment has been suggested in veterinary medicine. The goal of this study was to evaluate the immunoreactivity for estrogen and progesterone receptors and cell proliferative indexes in nine cases of canine IMC. Estrogen and progesterone receptors were negative in all cases. For cell proliferative indexes, the mean number of neoplastic cells staining positive for Ki-67 and PCNA were 4.47% and 20.81%, respectively. Lack of estrogen and progesterone receptor expression, suggesting an undifferentiated phenotype, in conjunction with a relatively high proliferative capacity reflected by Ki-67 and PCNA staining are features typical of many clinically aggressive neoplasms, including canine IMC.