Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 32
Filtrar
1.
Braz. j. biol ; 83: e247791, 2023. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1285637

Resumo

Abstract The growth of aquaculture sector is strongly dependent upon the continuous supply of inexpensive fish feed with balanced nutritional profile. However, fish meal (FM) is unable to satisfy this demand due to its scarce supply and high cost. In order to test the potential of cottonseed meal (CSM) as a fish meal replacer, a feeding trial of 12 weeks was conducted to check growth performance and proximate composition of Labeo rohita fingerlings. The protein ration of the test feed was satisfied by replacing FM with CSM at 0, 25, 50 and 75%. Sixteen test diets viz., TD1 (control), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 and TD16 were supplemented with citric acid (CA; 0 and 2.5%) and phytase (PHY; 0 and 750 FTU/kg) in a completely randomized design with 3×3 factorial arrangement. The highest weight gain (11.03g), weight gain% (249.21%), specific growth rate (1.39) and best feed conversion ratio (1.20) were recorded by fish fed with TD12. Furthermore, the same level increased the crude protein (59.26%) and fat (16.04%) being significantly different (p<0.05) than that of control. Conclusively, the addition of acidified phytase (CA; 2.5%, PHY; 750 FTU/kg) in TD12 (CSM=50%) led to the improved growth and proximate composition of L. rohita fingerlings.


Resumo O crescimento do setor de aquicultura é fortemente dependente do fornecimento contínuo de rações baratas para peixes com perfil nutricional equilibrado. Porém, a farinha de peixe (FM) não consegue atender a essa demanda devido à sua escassa oferta e alto custo. Com o objetivo de testar o potencial da farinha de semente de canola (MSC) como substituto da farinha de peixe, um ensaio alimentar de 12 semanas foi conduzido para verificar o desempenho de crescimento e a composição centesimal de alevinos de Labeo rohita. A ração de proteína da ração teste foi satisfeita substituindo FM por CSM em 0, 25, 50 e 75%. Dezesseis dietas de teste, viz., TD1 (controle), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 e TD16 foram suplementadas com ácido cítrico (CA; 0 e 2,5%) e fitase (PHY; 0 e 750 FTU / kg) em um delineamento inteiramente casualizado com arranjo fatorial 3 × 3. O maior ganho de peso (11,03g), % de ganho de peso (249,21%), taxa de crescimento específico (1,39) e melhor taxa de conversão alimentar (1,20) foram registrados por peixes alimentados com TD12. Além disso, o mesmo nível aumentou a proteína bruta (59,26%) e a gordura (16,04%), sendo significativamente diferente (p <0,05) do controle. Conclusivamente, a adição de fitase acidificada (CA; 2,5%, PHY; 750 FTU / kg) em TD12 (CSM = 50%) levou a um melhor crescimento e composição próxima de alevinos de L. rohita.


Assuntos
Animais , Cyprinidae , 6-Fitase , Óleo de Sementes de Algodão , Dieta/veterinária , Ração Animal/análise
2.
Braz. j. biol ; 83: e246568, 2023. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1278537

Resumo

Abstract Fishmeal; being a limited and costly feed ingredient is continuously been substituted with locally available plant proteins. However, the occurrence of anti-nutritional factors in plant meal suppresses its potential to be fully replaced. Therefore, in this study we aimed to study the synergistic effects of dietary additives like citric acid and phytase enzyme supplementation on growth performance and nutrient digestibility of Cirrhinus mrigala fingerlings. Canola meal (CM) was used as a test ingredient to replace fishmeal (FM) as; 0%, 25%, 50% and 75%. These four diets were further supplemented by varying levels of phytase (0 and 750 FTU kg-1) and citric acid (0% and 2.5%) to formulate total sixteen test diets as T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, T11, T12, T13, T14, T15 and T16. Each treatment contained three replicates; applied to fish groups having 15 fingerlings each; following 3×3 factorial arrangement. 1% of chromic oxide was added as an inert marker. Maximum weight gain% (288%) and the lowest value of FCR (1.07) were recorded when fish was fed on diet T12 as compared to fish fed control diet (T1). Similarly, optimum nutrient digestibility values such as crude protein (77%), crude fat (84%) and gross energy (70%) were noted on same level. It was concluded that 50% canola meal can optimally replace fishmeal when supplemented with phytase and citric acid at the levels of 750 FTU kg-1 and 2.5%, respectively.


Resumo A farinha de peixe, por ser um ingrediente alimentar limitado e caro, é continuamente substituída por proteínas vegetais disponíveis localmente. No entanto, a ocorrência de fatores antinutricionais na farinha de plantas suprime seu potencial de ser totalmente substituída. Portanto, neste estudo objetivamos estudar os efeitos sinérgicos de aditivos dietéticos como ácido cítrico e suplementação com enzima fitase sobre o desempenho de crescimento e digestibilidade de nutrientes de alevinos de Cirrhinus mrigala. A farinha de canola (CM) foi usada como ingrediente de teste para substituir a farinha de peixe (FM) como: 0%, 25%, 50% e 75%. Essas quatro dietas foram suplementadas por níveis variados de fitase (0 e 750 FTU kg-1) e ácido cítrico (0% e 2,5%) para formular um total de 16 dietas de teste como T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, T11, T12, T13, T14, T15 e T16. Cada tratamento continha três repetições; aplicado a grupos de peixes com 15 alevinos cada; seguindo o arranjo fatorial 3 × 3. 1% de óxido crômico foi adicionado como um marcador inerte. % de ganho de peso máximo (288%) e o valor mais baixo de FCR (1,07) foram registrados quando os peixes foram alimentados com dieta T12 em comparação com peixes alimentados com dieta controle (T1). Da mesma forma, valores ótimos de digestibilidade de nutrientes, como proteína bruta (77%), gordura bruta (84%) e energia bruta (70%) foram anotados no mesmo nível. Concluiu-se que 50% da farinha de canola pode substituir de forma ideal a farinha de peixe quando suplementada com fitase e ácido cítrico nos níveis de 750 FTU kg-1 e 2,5%, respectivamente.


Assuntos
Animais , 6-Fitase , Nutrientes , Galinhas , Ácido Cítrico , Suplementos Nutricionais , Dieta/veterinária , Digestão , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal
3.
Acta sci., Anim. sci ; 44: e54218, 2022. tab
Artigo em Inglês | VETINDEX | ID: biblio-1366580

Resumo

The aim of studywas to compare efficacy of 1-α(OH)D3alone or in combination with phytase and 1-α(OH)D3in combination of phytase and different concentration of cholecalciferol on performance, tibia parameters,andplasma minerals of quails fed Ca-P deficient diet. A total of 280 mixed sex 5-d-old quails were allocated to 7 treatments with 5 replicates.The vitamin supplement which incorporated to basal diet did not contain cholecalciferol.The dietary treatments were as follows: Ca-P deficient diet (basal diet); basal diet + 500 FTU phytase/kg of diet; basal diet + phytase + 5 µg of 1-α(OH)D3kg-1of diet;basal diet + phytase + 5 µg of 1-α(OH)D3and 250, 500, 750 and 1,000 IU of cholecalciferol kg-1of diet. The highest final body weight and the best feed conversion ratioobtained in the group supplemented with 1,000 IU cholecalciferol kg-1of diet (p < 0.05). Supplementation of 1-α(OH)D3 alone or in combination with phytase and phytase and different concentration of cholecalciferol could improve tibia parameters (p < 0.05). In conclusion, supplementation of 1-α(OH)D3alone to Ca-P deficient diet could maximize tibia mineralization, whereas it couldn't maximize performance, performance criteria were maximized by supplementation of 1,000 IU cholecalciferol kg-1of diet.(AU)


Assuntos
Animais , Colecalciferol , 6-Fitase , Coturnix/fisiologia , Tíbia
4.
R. bras. Ci. avíc. ; 23(3): eRBCA-2020-1395, 2021. tab
Artigo em Inglês | VETINDEX | ID: vti-32257

Resumo

Two experiments were carried out with the objective of evaluating the effect of superdosing of two phytases on performance, egg quality, digestive organ biometry and bone quality of light hens in the first (58 weeks) and second (87 weeks) productive cycle. In the first cycle, 270 light hens were used, in which a completely randomized design was adopted in a 2 x 2 + 1 factorial scheme (bacterial phytase or fungal phytase x 450 FTUs or 900 FTUs + control diet). In the second cycle, 270 hens were used, following the same design as the previous experiment. The performance and quality of the eggs were evaluated in both cycles, and the biometry of the digestive organs and bone characteristics were also evaluated in the first experiment. There was no effect in the comparison between the means for the performance and egg quality (in both experiments), or for the biometric variables and bone characteristics (first experiment). There was no significant interaction between the factors for any of the variables in the two experiments. In the first experiment, egg production was higher with bacterial phytase and egg weight with fungal phytase. The mineral matter showed greater value with 450 FTUs. It is recommended to use bacterial phytase produced from Escherichia coli as it improves the performance of light laying hens. The dosage of 450 FTUs improves the mineral content of light laying hens and the use of phytase in the laying hen diet implies a lower feed cost.(AU)


Assuntos
Animais , Galinhas/metabolismo , Galinhas/fisiologia , 6-Fitase/análise , 6-Fitase/biossíntese , 6-Fitase/química , Densidade Óssea , Ácido Fítico/química , Ciências da Nutrição , Escherichia coli/química
5.
R. cient. eletr. Med. Vet. ; (34): 14 p, jan. 2020. tab
Artigo em Português | VETINDEX | ID: vti-24846

Resumo

O presente trabalho teve como objetivo realizar revisão de literatura acerca da suplementação de fitase para tilápias. Por meio de consulta de periódicos científicos e livros. Foi realizado levantamento de dados, salientando a importância da utilização da fitase nos regimes intensivos de produção. Observou-se que a utilização da fitase pode promover efeitos positivos quando utilizada em rações formuladas com alimentos de origem vegetal. A fitase catalisa a hidrólise do fitato nos animais monogástricos, que não apresentam a enzima na forma endógena. A suplementação desta enzima beneficia o desenvolvimento dos peixes.(AU)


The present work aimed to perform a literature review about phytase supplementation for tilapia. By consulting scientific journals and books. Data collection was performed, highlighting the importance of the use of phytase in intensive production regimes. It was observed that the use of phytase can promote positive effects when used in diets formulated with foods of plant origin. Phytase catalyzes phytate hidrolysis in monogastric animals, which do not present the enzyme in endogenous form. Suplementation of this enzyme benefits fish development.(AU)


Assuntos
Animais , 6-Fitase/administração & dosagem , 6-Fitase/uso terapêutico , Tilápia , Eutrofização , Minerais na Dieta , Suplementos Nutricionais
6.
R. bras. Ci. avíc. ; 22(2): eRBCA-2019-1192, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-26874

Resumo

A trial was conducted to determine the effect of phytase (PHY) or a carbohydrase/protease cocktail (CPX) on broilers fed diets with two different levels of chloride (0.28% or 0.43%) created by altering dietary salt (NaCl) and sodium bicarbonate (NaHCO3). There were 6 combination dietary treatments (3 enzyme x 2 NaCl treatments) applied to 4 replicate pens. The treatments were as follows: Control diet (CON), CON+PHY and CON+CPX, with 0.5% or 0.25% NaCl. The 0.25% NaCl versions contained 0.35% sodium bicarbonate. The 0.5% salt versions had no sodium bicarbonate. Replicate pen BW, and feed consumption (FC) were measured at 1, 14, and 35 d, and mortality was weighed daily for feed conversion ratio (FCR) calculations. Feed consumption at 14 d tended to be lower (p 0.10) for CON+CPX diets compared to CON and CON+PHY diets. The birds fed CON+CPX diet consumed less feed but exhibited improved FCR in the presence of 0.5% NaCl at 14 d. The birds fed the CON, and CON+PHY diets exhibited higher BW at 14 d (p 0.05) and 35 d (p 0.01) of age than did CON+CPX birds. From 15 d to 35 d, birds fed the CON+CPX diet exhibited poorer BW gain (BWG) in the presence of 0.25% NaCl (p 0.05). In conclusion, Cl, as NaCl versus NaH2CO3, could affect CPX but not PHY feed enzyme function in broilers. Further, it may be suggested that certain feed enzymes may be best utilized at later broiler ages rather than in initial feeds.(AU)


Assuntos
Animais , Galinhas/crescimento & desenvolvimento , Bicarbonato de Sódio , 6-Fitase
7.
R. bras. Ci. avíc. ; 22(4): eRBCA-2020-1276, out. 2020. tab
Artigo em Inglês | VETINDEX | ID: vti-761977

Resumo

The aim of this study was to evaluate the effect of two types of phytases (the 1st produced from Escherichia coli and the 2nd from Aspergillus oryzae) with different dosages (300 and 900 Unit Phytase or FTU) on performance, organ biometry and bone quality of replacement pullets in the period of 8 to 17 weeks of age. A total of 288 Hy-Line White laying hens were used at 8 weeks of age. A completely randomized design was adopted in a 2 x 2 factorial scheme, totaling 4 treatments with 8 replicates of 9 birds each. Performance, biometric variables and bone characteristics were evaluated. There was no significant interaction between the factors for any of the evaluated variables. The conversion and the accumulated consumption were influenced (p 0.05) by the phytase dosages, with the highest results being observed for the consumption obtained with 300 FTU, and the best results for the feed conversion obtained with the dosage of 900 FTU. The relative weight of the liver was influenced by the treatments (p 0.05), presenting higher values with bacterial phytase and a dosage of 900 FTU. The sternum length and tibial deformity were influenced by fungal phytase (p 0.05). It is concluded that the use of 900 FTU superdosing and fungal phytase improves the performance and bone characteristics of light replacement pullets.(AU)


Assuntos
Animais , Galinhas/anatomia & histologia , Galinhas/genética , Galinhas/fisiologia , 6-Fitase/análise , 6-Fitase/química , Biometria
8.
R. bras. Ci. avíc. ; 22(1): eRBCA-2019-1178, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-29146

Resumo

This study aimed to evaluate the effect of using 500 FTU/kg of hybrid phytase (created from three bacteria - Natuphos E), with or without xylanase and glucanase (560 TXU/kg; 250 TGU/kg) - Natugrain TS, in corn and soybean meal-based diets, with nutritional reductions in metabolizable energy (ME), Ca, and available phosphorus (AP), on performance parameters and production costs in broiler chickens. The 1875chickens were housed in boxes and distributed among 5 treatments with 15 replicates of 25 chickens each. The experiment included a positive control (the diet of which met the nutritional requirements of the birds), two negative controls (with ME reductions of 70 and 100 kcal/kg, and fixed reductions in Ca [0.16%] and AP [0.15%]), and two treatments with identical nutritional reductions in addition to enzyme supplementation. The treatments included the following: PC= positive control (basal diet (BD) corn and soybean meal); R_70 = BD with reduction of 70 kcal/kg, Ca and AP;R_100 = BD with reduction of 100 kcal, Ca and AP; R_70 + P =BD with reduction of 70 kcal/kg, Ca and AP + phytase (500 FTU/kg); R_100 + P + XG =BD with reduction of 100 kcal/kg, Ca and AP + phytase (500 FTU/kg) + xylanase (560 TXU/kg) + glucanase (250 TGU/kg). Performance parameters, carcass yield, and production costs (USD/ton chilled carcass) were evaluated. In conclusion, the reductions of 70 kcal/kg, 0.16% Ca, and 0.15% AP did not affect performance in chickens over 42 days, if diets were supplemented with hybrid phytase (500 FTU/kg). Supplementation with hybrid phytase and carbohydrases in diets with reductions of 100 kcal/kg, 0.16% Ca, and 0.15% AP led to lower production costs.(AU)


Assuntos
Animais , Galinhas/anatomia & histologia , Galinhas/crescimento & desenvolvimento , 6-Fitase/biossíntese , Zea mays , Glycine max , Fósforo
9.
Semina Ci. agr. ; 40(2): 767-780, Mar.-Apr. 2019. tab
Artigo em Inglês | VETINDEX | ID: vti-19563

Resumo

Although phytase has been researched, new enzymes have been produced, leading to different animal responses. In this scenario, the present study proposes to evaluate the inclusion of a bacterial phytase produced by Escherichia coli in broiler diets based on corn and soybean meal, with or without nutrient reductions, on the performance, nutrient digestibility, phosphorus bioavailability, and bone minerals of those animals and on the economic viability of this practice. A total of 896 male broiler chickens were distributed into 32 experimental units, each housing 28 broilers. The experiment was set up as a completely randomized design with four treatments (Positive Control (PC)- diet meeting the nutritional requirements of the broiler chickens; Negative Control (NC) with reductions of 100kcal/kg of ME, 0.14% avP and 0.11% tCa; NC + phytase (500 FTU/kg); PC + phytase (500FTU/kg)) and 8 replicates. Phytase increased (p < 0.05) feed intake and body weight gain and improved feed conversion ratio in starter (1 to 21 days) and total (1 to 42 days) phases, respectively, compared with Negative Control diet without supplementation. The Negative Control + phytase diet also led to a feed intake similar to Positive Control in the starter and total phases. The inclusion of phytase without nutrient reductions improved (p < 0.05) feed conversion ratio in the starter phase, compared to...(AU)


Embora a fitase tenha sido estudada, novas enzimas foram produzidas, levando a respostas animais diferentes. Nesse sentido, o presente estudo teve como objetivo avaliar a inclusão de fitase bacteriana produzida por Escherichia coli em dietas à base de milho e farelo de soja, com ou sem redução de nutrientes sobre o desempenho, digestibilidade dos nutrientes, biodisponibilidade de fósforo, minerais ósseos e viabilidade econômica. Foram distribuídos 896 frangos de corte machos em 32 unidades experimentais, cada unidade constituída por 28 frangos. O delineamento foi inteiramente casualizado com quatro tratamentos (Controle Positivo (CP) - dieta que satisfaz os requisitos nutricionais das aves; Controle Negativo (CN) - reduções de 100 kcal/kg EM; 0,14% avP e 0,11% tCa); CN + fitase (500 FTU/kg); CP + fitase (500 FTU/kg)) e 8 repetições. A fitase aumentou (p < 0,05) o consumo de ração, o ganho de peso médio e melhorou a conversão alimentar nas fases inicial (1 a 21 dias) e total (1 a 42 dias), respectivamente, em comparação com a dieta Controle Negativo, sem suplementação. O Controle Negativo + fitase também apresentou consumo de ração semelhante ao Controle Positivo nas fases inicial e total. A inclusão da fitase sem redução dos requerimentos nutricionais (Controle positivo + fitase) melhorou (p < 0,05) a conversão alimentar na fase inicial, em comparação com a dieta Controle...(AU)


Assuntos
Animais , Enzimas/administração & dosagem , Enzimas/análise , 6-Fitase/administração & dosagem , 6-Fitase/análise , Disponibilidade Biológica , Galinhas , Escherichia coli , Cálcio da Dieta , Fósforo na Dieta , Suplementos Nutricionais
10.
Acta Sci. Anim. Sci. ; 38(4): 355-359, oct.-dec. 2016. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-13660

Resumo

This study aimed to evaluate the digestibility of plant energetic ingredients, corn, sorghum and wheat bran, with and without phytase supplementation, in silver catfish (Rhamdia voulezi). The experimental design was completely randomized, represented by eight treatments, which consisted of the development of a reference diet and a test diet for each ingredient evaluated (corn, sorghum and wheat bran), with and without supplemental phytase. Diets were formulated so as to contain 70% reference feed and 30% test ingredient. The digestibility coefficients of dry matter and crude protein were significantly influenced only in relation to the evaluated ingredients. Phytase influenced the digestibility coefficients of energy and phosphorus, with interaction between food and the enzyme addition. The interaction between these two factors shows that the phytase efficiency is dependent on the type of vegetable ingredient used in the diet. The results suggest the supplementation of phytase for a higher nutrient digestibility of corn, sorghum and wheat bran by silver catifish (R. voulezi).(AU)


O objetivo do presente estudo foi avaliar a digestibilidade dos ingredientes energéticos vegetais, milho, sorgo e farelo de trigo, com e sem suplementação de fitase pelo jundiá (Rhamdia voulezi). O delineamento experimental foi inteiramente casualizado, representado por oito tratamentos, os quais consistiram na elaboração de uma dieta-referência e uma dieta- teste para cada ingrediente avaliado (milho, sorgo e farelo de trigo), com e sem a suplementação de fitase. As dietas foram fabricadas de modo que continham 70% da ração referência e 30% do ingrediente teste. Os coeficientes de digestibilidade da matéria seca e proteína bruta foram influenciados significativamente somente em relação aos ingredientes avaliados. A fitase influenciou nos coeficientes de digestibilidade da energia e do fósforo, onde também foi observada a interação entre os alimentos e a inclusão da enzima. A interação entre esses dois fatores demonstra que a eficiência da fitase é dependente do tipo de ingrediente vegetal utilizado na dieta. Pelos resultados obtidos sugere-se a suplementação de fitase visando maior digestibilidade dos nutrientes, do milho, sorgo e farelo de trigo pelo jundiá (R. voulezi).(AU)


Assuntos
6-Fitase/efeitos adversos , 6-Fitase/análise , Digestão/fisiologia , Análise de Alimentos , Zea mays , Sorghum
11.
Braz. J. Microbiol. ; 46(1): 251-260, Jan.- Mar. 2015. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-481354

Resumo

An Aspergillus niger UFV-1 phytase was characterized and made available for industrial application. The enzyme was purified via ultrafiltration followed by acid precipitation, ion exchange and gel filtration chromatography. This protein exhibited a molecular mass of 161 kDa in gel filtration and 81 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), indicating that it may be a dimer. It presented an optimum temperature of 60 °C and optimum pH of 2.0. The KM for sodium phytate hydrolysis was 30.9 mM, while the kcat and kcat/KM were 1.46 ×105 s−1 and 4.7 × 106 s−1.M−1, respectively. The purified phytase exhibited broad specificity on a range of phosphorylated compounds, presenting activity on sodium phytate, p-NPP, 2- naphthylphosphate, 1- naphthylphosphate, ATP, phenyl-phosphate, glucose-6-phosphate, calcium phytate and other substrates. Enzymatic activity was slightly inhibited by Mg2+, Cd2+, K+ and Ca2+, and it was drastically inhibited by F−. The enzyme displayed high thermostability, retaining more than 90% activity at 60 °C during 120 h and displayed a t1/2 of 94.5 h and 6.2 h at 70 °C and 80 °C, respectively. The enzyme demonstrated strong resistance toward pepsin and trypsin, and it retained more than 90% residual activity for both enzymes after 1 h treatment. Additionally, the enzyme efficiently hydrolyzed phytate in livestock feed, liberating 15.3 μmol phosphate/mL after 2.5 h of treatment.(AU)


Assuntos
6-Fitase/isolamento & purificação , 6-Fitase/metabolismo , /enzimologia , 6-Fitase/química , Precipitação Química , Cromatografia em Gel , Cromatografia por Troca Iônica , Eletroforese em Gel de Poliacrilamida
12.
Acta sci. vet. (Online) ; 43: 1-5, 2015. tab
Artigo em Inglês | VETINDEX | ID: vti-23744

Resumo

Background: Addition of the phytase enzyme to the diet of monogastric animals is an effective and practical method for both utilization of phytate-P and P disposal. Besides hydrolytic effects on phytate, phytase may also improve the availability of minerals. Alkaline phosphatase (ALP) plays an important role in bone mineralization. Since dietary P levels decrease, serum ALP level has been shown to increase. It was reported that pigs and broilers fed phytase had lower serum ALP level. The present study aimed to investigate the effect of microbial phytase on serum calcium (Ca), inorganic phosphorus (Pi) levels and ALP activities in broilers fed diets containing different P levels.Materials, Methods & Results: A total of 144 one-day-old male broiler chicks (Cobb 500 stain) were used in this study. The birds were housed in metabolic cages (33 cm × 33 cm × 40 cm) under environmentally controlled conditions for 42 days. The fluorescent lights were on for 24 h each day. Diets and water were provided ad libitum. Starter diet was given in the first three weeks of the study, and grower diet was given from the beginning of the fourth to the end of the sixth week. The study was designed as a 3 × 2 factorial arrangement with three levels, low, medium and high, of nonphytate phosphorus (nPP) (0.18, 0.30 and 0.45 %), and two levels of phytase (0 and 600 U/kg diet). Blood samples were collected from 5 broilers in each group on days 21 and 42 and analyzed for serum Ca and Pi levels and ALP activities. Serum Ca concentrations were affected only by dietary P levels between 0-21 days (P < 0.05), but there were no differences between groups for serum Ca concentrations for 22-42 days. Phytase supplementation had no effect on serum Ca concentrations. During the experiment, serum P concentrations increased linearly depending on dietary P levels.[...](AU)


Assuntos
Animais , Galinhas/sangue , 6-Fitase/sangue , 6-Fitase/uso terapêutico , Cálcio/sangue , Fosfatase Alcalina , Fósforo na Dieta/sangue
13.
B. Inst. Pesca ; 41(4): 1025-1032, Out-Dez. 2015. tab, graf
Artigo em Português | VETINDEX | ID: vti-27317

Resumo

This research was carried out to compare the effect of phytase on performance, and in the incorporation of phosphorus in tanks with tambaqui. Four isoproteic (30%) and isocaloric diets were used: one standard (control), without phytase supplementation, and other three with different phytase levels (1,000, 1,500 and 2,000 uf kg-1), with three replication each diet, during 45 days. The results demonstrated that the use of phytase in diets had no effect on weight gain, feed conversion and fish survival, but can reduce fish phosphorus excretion by 21%, minimizing the impacts caused by this nutrient.(AU)


Esta pesquisa foi realizada com o objetivo de comparar a ação da fitase no desempenho de tambaqui (Colossoma macropomum) e na incorporação de fósforo na água em tanques. Foram utilizadas quatro rações, isoproteicas (30% PB) e isocalóricas, sendo uma padrão (controle), sem suplementação de fitase, e três com diferentes níveis de fitase (1.000, 1.500 e 2.000 uf kg-1), com três repetições, por um período de 45 dias. Os resultados comprovaram que a utilização de fitase nas rações não interferiu no ganho de peso, conversão alimentar e sobrevivência dos peixes, mas pode reduzir a excreção de fósforo pelos animais em 21%, minimizando o impacto ambiental gerado por este nutriente.(AU)


Assuntos
Animais , 6-Fitase , Characidae , Fósforo na Dieta , Ração Animal , Qualidade da Água
14.
R. bras. Saúde Prod. Anim. ; 15(2): 493-503, abr.-jun. 2014. ilus
Artigo em Português | VETINDEX | ID: vti-16779

Resumo

The objective of thisstudywas to evaluatethe effect ofreducedcalcium, phosphorus, energy andamino aciddietsupplemented withphytase forbroilersin the growth phase(14 to28 days)on the ofnitrogen, calciumand phosphorus balance. We distributed392male broilersofCobb, in a completely randomizedin a factorial3 x2 + 1Reduced levelofCaandP; Reduced levels ofamino acidsand energylevelsreducedCa, P, andamino acids x with and withoutenzyme+positive control), seven treatmentswitheight replicatesof sevenchicks each. In the diets with phytasewas used 500 FTU of phytase/ kg diet.Data weresubmitted to varianceanalysisandaverages werecomparedby theF test fororthogonal contrasts. Through the results can be seen that the enzyme phytase used thus of 21,34% reduction in calcium excretion end apparent retention ratio of calcium to 20,16%. Similarly, reductions of nitrogen excretion by 7,56% and phosphorus excretion by 21,34%, the environmental impact reducing caused by the excretion of these nutrientes of these nutrientes and increasing the apparent retention ratio of 9,41 and 33,94% in average, respectively.(AU)


Objetivou-se com este estudo avaliar o efeito da redução de nutrientes e energia suplementada ou não com fitase para frango de corte na fase 14 a 24 dias de idade no consumo, excreção e balanço de nitrogênio, de cálcio e de fósforo. Foram distribuídos 392 frangos de corte em delineamento inteiramente casualizado, utilizando-se arranjo fatorial 3 x 2 + 1 que consistiram de nível reduzido de cálcio e fósforo; níveis reduzidos de aminoácidos e energia; níveis reduzidos cálcio, fósforo, aminoácidos e energia x com e sem enzima + controle positivo, totalizando sete tratamentos com oito repetições de sete aves por unidade experimental. Nas dietas com fitase utilizou-se 500 FTU de fitase/kg de ração. Os dados foram submetidos à análise de variância e as médias comparadas por contrastes ortogonais através do teste F. Os resultados demonstram que a enzima fitase utilizada permitiu diminuir a quantidade de nutriente e energia das rações, proporcionando redução de 21,34% na excreção de cálcio e balanço de cálcio de 20,16%. Similarmente, reduções das quantidades de excreção de nitrogênio em 7,56% e fósforo em 21,34%, diminuindo a excreção desses nutrientes e aumentando o balanço de nitrogênio e fósforo em 9,41 e 33,94% em média, respectivamente.(AU)


Assuntos
Animais , Aves Domésticas/genética , Aves Domésticas/metabolismo , Ração Animal/análise , Ração Animal/classificação , Inquéritos sobre Dietas , 6-Fitase/análise
15.
Arq. bras. med. vet. zootec ; 66(1): 235-243, fev. 2014. ilus, tab
Artigo em Português | VETINDEX | ID: vti-10293

Resumo

O objetivo deste trabalho foi avaliar os efeitos da inclusão de níveis crescentes de enzima fitase em dietas para suínos em fase de crescimento, por meio do uso de modelagem matemática. Foram utilizados dados de 20 leitões mestiços, machos castrados, pesando em média, 26,8kg. Os animais ficaram alojados em gaiolas metabólicas individuais para a coleta de fezes e urina, onde permaneceram por um período de 17 dias. Utilizou-se o delineamento experimental de blocos ao acaso, com cinco tratamentos e quatro repetições. A dieta experimental fornecida aos leitões foi à base de milho e farelo de soja, suplementada com cinco níveis crescentes de enzima fitase (0, 250, 500, 750 e 1000UF/kg), correspondendo a 0; 0,01; 0,02; 0,03 e 0,04%, respectivamente. As variáveis avaliadas foram: ingestão, excreção, fluxo e refluxo do P nos compartimentos (trato digestivo, corrente sanguínea, tecidos moles e ossos). A enzima fitase não interferiu no P consumido (P>0,05 (F10), no P excretado na urina (F02), no fluxo e refluxo do P nos ossos (F32 e F23) e nos tecidos moles (F42 e F24), entretanto observou-se redução no P excretado nas fezes (F01) em 8,92%; 26,76%; 22,53% e 28,64% para os níveis 0, 250, 500, 750 e 1000UF/kg, respectivamente, e efeito linear positivo para o P endógeno (F12). Pode-se utilizar dietas à base de milho e farelo de soja com 50% de P por fosfato bicálcico, adicionando-se 250UF/kg de dieta para suínos em crescimento, o que reduz em 27% as excreções de P nas fezes.(AU)


The purpose of this study was to evaluate the effects of including increasing levels of phytase enzyme in pig diets for growing pigs, using the mathematical model. Data from 20 crossbred male piglets, castrated and weighing 26.80 kg on average was used. The animals were housed in individual metabolic cages to collect feces and urine in a 17 day period. A randomized block experimental design containing five treatments and four repetitions was used. The experimental diet provided to piglets contained corn and soybean and was supplemented with five increasing levels of phytase enzyme (0, 250, 500, 750 and 1000 UF/kg), corresponding to 0.00 %, 0.01 %, 0.02 %, 0.03 % and 0.04 % respectively. The variables evaluated were: intake, excretion, output flow of P in the digestive tract, bloodstream, bones and soft tissues. The phytase enzyme did not affect the P intake (P>0.05 (F 10), the P excreted in urine (F02) and the output flow of P in the bones (F32 e F23) and soft tissue (F42 e F24) , however, there was a reduction in P excreted in feces (F01) of 8.92 %, 26.76 %, 22.53 % and 28.64 % to the levels 0, 250, 500, 750 e 1000UF/kg, respectively and showed a positive linear effect (P<0.08) for the endogenous P (F12) . Corn and soybean meal based diets can be used with 50% of P by dicalcium phosphate, adding 250UF/kg diet for growing pigs, and may cause a reduction of 27 % of P excretion in feces.(AU)


Assuntos
Animais , Radioisótopos/análise , Dieta , 6-Fitase , Fósforo/análise , Suínos/classificação
16.
Arq. bras. med. vet. zootec ; 65(1): 231-240, 2013. tab
Artigo em Português | VETINDEX | ID: vti-9836

Resumo

Avaliou-se o efeito de estratégias nutricionais sobre o desempenho e as características de carcaça de suínos dos 30 aos 100kg. Foram utilizados 120 suínos, distribuídos em delineamento experimental de blocos ao acaso, com cinco tratamentos e oito repetições de três animais por unidade experimental. Os tratamentos usados foram: dieta-controle = dieta com alta proteína bruta sem aminoácidos industriais; dieta baixa PB= dieta com baixa proteína bruta com aminoácidos industriais; dieta fitase = dieta-controle com suplementação de fitase; dieta orgânica = dieta-controle suplementada com minerais inorgânico-orgânicos; e dieta composta = dieta com baixa proteína bruta suplementada com aminoácidos industriais, fitase e minerais inorgânico-orgânicos. As dietas avaliadas não alteraram o desempenho e as características de carcaça dos suínos em comparação aos animais da dieta-controle. Conclui-se que a redução de proteína bruta, a suplementação com fitase e minerais inorgânico-orgânicos podem ser adotadas por não alterarem significativamente o desempenho nas fases de crescimento e terminação e as características de carcaças.(AU)


The objective was to evaluate the effect of different nutritional strategies on performance and carcass traits of pigs from 30 to 100kg. One hundred and twenty pigs were used, distributed in randomized blocks, with five treatments and eight replicates of three animals per experimental unit. Treatments were: control diet = corn-soybean based diet without synthetic amino acids; low crude protein diet = corn-soybean based diet with low protein and synthetic amino acids; phytase diet = control diet supplemented with phytase; organic diet = control diet supplemented with inorganic and organic minerals; and combination diet = diet with low protein supplemented with phytase and inorganic-organic minerals. Means were compared with the control diet by the Dunnett test. The diets evaluated did not alter the performance of pigs when compared to pigs fed the control diet. The reduction of crude protein, supplementation with phytase and inorganic-organic minerals can be adopted without significantly changing performance during the growing-finishing and carcass traits at 100kg.(AU)


Assuntos
Animais , Nutrientes/efeitos adversos , Nutrientes/análise , Suínos , Fenômenos Fisiológicos da Nutrição Animal , 6-Fitase
17.
Bol. ind. anim. (Impr.) ; 70(2): 149-157, 2013. tab
Artigo em Português | VETINDEX | ID: biblio-1434331

Resumo

Objetivou-se com esta pesquisa avaliar o desempenho e a qualidade óssea de poedeiras na fase de pós pico de postura, alimentadas com dietas contendo níveis de fósforo e fitase. Foi realizado um experimento com 384 poedeiras da linhagem Hy-line branca distribuídas em delineamento inteiramente casualizado em esquema fatorial 4 x 3, com 4 níveis de fósforo disponível e 3 níveis de fitase. O período experimental foi dividido em quatro ciclos de 28 dias, ao final de cada ciclo experimental foram determinados o consumo de ração, produção de ovos, peso de ovos, conversão alimentar, mortalidade, peso médio do ovo, espessura de casca, unidades Haugh e gravidade específica dos ovos. Ao final do período experimental foram determinadas as quantidades de cálcio e fósforo excretadas pelo método de coleta total de excretas e uma ave por unidade experimental foi sacrificada por deslocamento da articulação crânio-cervical para retirada dos ossos e avaliação da largura, comprimento e índice de robustez do fêmur e tíbia. Houve interação entre os níveis de fósforo e fitase para consumo de ração, conversão alimentar e percentual de postura. Para os níveis de inclusão de fitase todas as variáveis de qualidade de ovo não apresentaram diferenças significativas. Os tratamentos não afetaram as características ósseas das poedeiras.


The objective of this research was to evaluate the performance and bone quality of laying hens after peak production fed diets containing phosphorus levels and phytase. An experiment was conducted with 384 Hy-line distributed in a completely randomized in a factorial 4 x 3 with 4 levels of available phosphorus and 3 levels of phytase. The experimental period was divided into four periods of 28 days, at the end of each cycle were determined experimental feed intake, egg production, egg weight, feed conversion, mortality, and average egg weight, shell thickness, Haugh units and specific gravity. At the end of the experimental period were determined amounts of calcium and phosphorus excreted by the method of total excreta collection and a fowl per experimental unit was sacrificed for collection of bones and evaluation of width, length and level of robustness from femur and tibia. There was interaction between phosphorus levels and phytase on feed intake, feed conversion and percentage of posture. For inclusion levels of phytase all egg quality variables showed no significant differences. The treatments did not affect bone characteristics of laying hens.


Assuntos
Animais , Feminino , Cálcio da Dieta/administração & dosagem , Galinhas/fisiologia , Fósforo na Dieta/administração & dosagem , 6-Fitase/administração & dosagem , Ovos/análise , Ração Animal/análise , Desenvolvimento Ósseo
18.
Arq. bras. med. vet. zootec ; 65(6): 1831-1835, dez. 2013. tab
Artigo em Português | VETINDEX | ID: vti-10135

Resumo

Foram utilizados 504 pintos de linhagem comercial (Ag Ross 308) para frangos de corte de um dia de idade, distribuídos em 12 tratamentos com seis repetições. O delineamento experimental utilizado foi de blocos ao acaso em esquema fatorial 2x2x3, com dois níveis de fósforo disponível (0,45 e 0,34%), dois níveis de fitase (0 e 1200 FTU/kg) e três níveis de proteína bruta (22,5; 20,5 e 18,5%). A porcentagem de cálcio e fósforo nas tíbias foi influenciada significativamente pelos níveis de proteína e de fósforo na dieta com o uso da fitase. Os teores de matéria mineral nas tíbias apresentaram efeito linear com o uso da enzima e efeito quadrático com a ausência dela, apresentando maior valor com o nível de 22,5% de proteína na dieta. Já com os maiores níveis de fósforo houve efeito linear entre os níveis de proteína bruta na dieta e os pesos da matéria mineral nas tíbias, ou seja, quanto maior o nível de proteína, menor o peso da matéria mineral.(AU)


504 (five hundred four) 1 day old male chicks from a commercial broiler line (Ag Ross 308) were used, distributed in 12 treatments, with 6 replicates per treatment. The experimental design was casually blocked and treatments were organized in a 2x2x3 factorial arrangement: two available phosphorus levels (0.45 and 0.34%), two phytase inclusion levels (0 and 1200 FTU/kg) and three crude protein levels (22.5; 20.5 and 18.5%). Tibia calcium percentage was influenced by protein and phosphorus levels in the diet, when using phytase. Tibia ash levels showed a linear effect when enzymes were added and showed a quadractic effect without it, and the higher value was observed with 22.5% crude protein in the diet. With higher phosphorus levels a linear effect was observed between crude protein in the diet and tibia ash weights, as a higher protein level had lower tíbia ash weight.(AU)


Assuntos
Animais , Ração Animal , Dieta/métodos , 6-Fitase , Fósforo , Enzimas , Galinhas/classificação , Aves Domésticas/métodos
19.
R. bras. Saúde Prod. Anim. ; 14(3): 518-526, July.-Sept.2013. tab, graf
Artigo em Português | VETINDEX | ID: vti-715269

Resumo

This study had as purpose to evaluate the partition of P excreted in the faeces and urine of growing pigs fed with diets containing different levels of inclusion of phytase enzyme, through the use of radioisotope P-32.). Twenty pigs crossbred barrows were evaluated in a randomized block design experiment, with five treatments, 0; 250; 500; 750 and 1000PUkg diet, and four replicates. The animals were kept in metabolic cages for a 10-day adaptation period and seven days for total collection of feces and urine. Blood samples were taken for five days. On the first day of the collection period each animal was injected intravenously with 7.4MBq 32P phytase. The use of Phytase in diets for pigs provides reduction of P total and soluble excreted in the feces. On diets for growing pigs containing corn and soybean meal, you can reduce by 50% of the supplementation of the dicalcium phosphates, adding 500UF/kg diet, without causing damage to animal physiological system.(AU)


Este estudo teve como propósito avaliar a partição do P excretados nas fezes e urina de suínos em fase de crescimento, alimentados com dietas contendo diferentes níveis de inclusão de enzima fitase, através do uso do radioisótopo P-32. Foram utilizados 20 suínos, machos castrados, em delineamento de blocos ao acaso, com cinco tratamentos 0; 250; 500; 750 e 1000UF/kg da ração - e quatro repetições. Os animais foram alojados em gaiolas metabólicas por 17 dias, sendo dez dias para adaptação e sete dias para coleta de fezes e urina. Amostras de sangue foram coletadas por cinco dias. No primeiro dia da fase experimental, cada animal recebeu por via endovenosa uma solução radioativa com 7,4MBq de 32P. O uso de fitase em dietas para suínos proporciona redução do P total e solúvel excretados nas fezes. Pode-se reduzir em 50% a suplementação de P por fosfato bicálcico nas dietas para suínos em crescimento, adicionando 500UF/kg de dieta, sem provocar prejuízos ao sistema fisiológicos dos animais.(AU)


Assuntos
Animais , Suínos/crescimento & desenvolvimento , Suínos/metabolismo , Ração Animal/efeitos adversos , Ração Animal/análise , 6-Fitase , Fósforo
20.
R. bras. Ci. avíc. ; 15(4): 353-364, Dec. 2013. tab
Artigo em Inglês | VETINDEX | ID: vti-28740

Resumo

The objective of this study was to evaluate the effect of diets containing different calcium and phytase levels on the nutritional balance of broilers.A total of 108 male AG Ross 308 broilers were used in each of the replicates phases used in the study: starter (1-21 days), grower (29-35 days) and finisher (36-42 days). A completely randomized experimental design in a 3 x 2 factorial arrangement, with three phytase levels (0, 600 or 1200 FTU/kg) and two calcium levels (0.94 and 0.66%; 0.84 and 0.59%; e 0.78 and 0.54% in the starter, grower, and finisher phases, respectively), totaling six treatments with six replicates each.The experimental feeds also contained reduced available phosphorus levels and minimum crude protein level.The method of total excreta collection was applied to determine dry matter, nitrogen, gross energy, calcium, and phosphorus nutritional balance.Reducing dietary calcium levels to 0.66, 0.59, and 0.54%, and using 0.27, 0.22, and 0.20% available phosphorus, 18.50, 17.50, and 16.00% crude protein, and 600 FTU phytase/kg in the diets of the starter, grower, and finisher phases, respectively, allow higher nitrogen, phosphorus, and calcium retention in broilers.During the grower phase, there was positive linear effect of increasing phytase levels in high-calcium diets on AMEn utilization, and the optimal phytase levels for low-calcium diets was 598.71 FTU/kg.In the finisher phase, the best AMEn utilization was obtained with the high-calcium diets.(AU)


Assuntos
Animais , Valor Nutritivo , Ração Animal , Galinhas/metabolismo , Cálcio , 6-Fitase
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA