Type C botulism outbreak in free-ranging waterfowl in Goiás / Surto de botulismo tipo C em aves aquáticas de vida livre em Goiás

Botulism is generally a fatal disease caused by ingestion of neurotoxins produced by Clostridium botulinum. The present study describes the epidemiological, clinical, and laboratory aspects of a type C botulism outbreak in free-living aquatic birds residing in an urban park in Quirinópolis, Goiás, Brazil. Among a population of approximately 80 waterfowl, a total of 30 birds, including ducks (Cairina moschata), teals (Anas platyrhynchos), and geese (Anser cygnoides), died within 10 days. Of these, six birds showed signs of flaccid paralysis of the pelvic limbs, eyelids, neck, and wings. To confirm the suspicion of botulism, four lake water samples, two samples of the feed consumed by the birds, and samples of serum, intestinal content, stomach content, and liver tissue from two teals that died after presenting clinical signs were analyzed. Using bioassay and neutralization with homologous antitoxin in mice, it was possible to detect the presence of botulinum toxin type C in a water sample and in the intestinal content of one of the necropsied teals. Additionally, the presence of C. botulinum type C was identified in the lake water using polymerase chain reaction. Based on the clinical signs and laboratory results, a diagnosis of botulism caused by botulinum toxin type C was confirmed with probable transmission by lake water.

O botulismo é uma doença geralmente fatal, causada pela ingestão de neurotoxinas produzidas pelo Clostridium botulinum. O presente estudo descreve os aspectos epidemiológicos, clínicos e laboratoriais de um surto de botulismo tipo C em aves aquáticas de vida livre habitantes de parque urbano em Quirinópolis, Goiás. De uma população de cerca de 80 aves aquáticas, um total de 30 aves, entre patos (Cairina moschata), marrecos (Anas platyrhynchos) e gansos (Anser cygnoides), morreram no intervalo de 10 dias. Destes, seis aves apresentaram sinais de paralisia flácida de membros pélvicos, pálpebras, pescoço e asas. Para confirmar a suspeita de botulismo, foram analisadas quatro amostras da água do lago, duas amostras da ração consumida pelas aves e amostras de soro, conteúdo intestinal, conteúdo estomacal e fígado de dois marrecos que morreram após apresentarem os sinais clínicos. Pelo bioensaio e neutralização com antitoxina homóloga em camundongos foi possível detectar a presença de toxina botulínica tipo C em uma amostra de água e no conteúdo intestinal de um dos marrecos necropsiados. Adicionalmente, pela reação em cadeia da polimerase identificou-se a presença de C. botulinum tipo C na água do lago. Com base nos sinais clínicos e resultados laboratoriais estabeleceu-se o diagnóstico de botulismo causado pela toxina botulínica tipo C e veiculada provavelmente pela água do lago.

Valores normais da atividade da colinesterase plasmática em anseriformes, com destaque para Alopochen aegyptiacus / Normal values of plasma cholinesterase activity in anseriformes, with emphasis on Alopochen aegyptiacus

A inibição da enzima colinesterase plasmática (BChE) pode ser utilizada como biomarcador para os efeitos da intoxicação por organofosforados e carbamatos. Nas aves, esta inibição ocorre de forma mais acentuada que nos mamíferos, porém poucos são os trabalhos publicados nestas espécies. O objetivo do estudo fo a dosagem da BChE em gansos-egípcios (Alopochen aegyptiacus) e nos anseriformes domésticos: gansos-domésticos (Anser anser domesticus) e marrecos (Anas platyrhynchos domesticus), para o estabelecimento de valores de referência normais. O trabalho possui ineditismo com relação à determinação desta enzima nos gansos-egípcios e domésticos. Os gansos e marrecos são mantidos em confinamento com fornecimento de alimentos e água ad libitum e em espaço adequado à sua manutenção no Instituto Adolfo Lutz (IAL), com a finalidade de fornecimento de sangue para a alimentação de triatomídeos do insetário de criação no Núcleo de Parasitoses Sistêmicas. Nos Alopochen aegyptiacus a média e o desvio padrão da BChE foram de 1.868 + 263,6 U/L, nos Anser anser domesticus 2.311 + 673,2 U/L e nos Anas platyrhynchos domesticus 4.290 + 86,11 U/L. (AU)

The inhibition of the plasma cholinesterase enzyme (BChE) can be used as a biomarker for the effects of intoxication by organophosphates and carbamates. In birds, this inhibition is more pronounced than in mammals, however there are few specific studies were conducted in this field. The aim of this study was to measure BChE in Egyptian geese (Alopochen aegyptiacus) and domestic anseriforms: domestic geese (Anser anser domesticus) and mallards (Anas platyrhynchos domesticus), not exposed to pesticides, for the establishment of normal values. The work is unprecedented regarding the determination of this enzyme in egyptian geese and domestic geese. Geese and mallards are kept in confinement with ad liditum food and water supply and in adequate space for their maintenance at the Adolfo Lutz Institute (IAL), for the purpose of supplying blood for the feeding of triatomines from the insectary of the Nucleus of Systemic Parasitoses. In Alopochen aegyptiacus the mean and standard deviation of BChE were 1,868 + 263,6 U/L, in Anser anser domesticus 2,311 + 673,2 U/L and in Anas platyrhynchos domesticus 4,290 + 86.11 U/L. (AU)

Avipoxvirus detected in tumor-like lesions in a white-faced whistling duck (Dendrocygna viduata) / Avipoxvirus detectado em lesões tipo tumorais em uma marreca-irerê (Dendrocygna viduata)

Avipoxvirus is the etiological agent of the avian pox, a well-known disease of captive and wild birds, and it has been associated with tumor-like lesions in some avian species. A white-faced whistling duck (Dendrocygna viduata) raised in captivity was referred to a Veterinary Teaching Hospital in Northeast due to cutaneous nodules present in both wings. A few days after the clinical examination, the animal died naturally. Once submitted to necropsy, histopathological evaluation of the lesions revealed clusters of proliferating epithelial cells expanding toward the dermis. Some of these cells had round, well-defined, intracytoplasmic eosinophilic material suggestive of poxvirus inclusion (Bollinger bodies). PCR performed on the DNA extracted from tissue samples amplified a fragment of the 4b core protein gene (fpv167), which was purified and sequenced. This fragment of Avipoxvirus DNA present in these tumor-like lesions showed high genetic homology (100.0%) with other poxviruses detected in different avian species in several countries, but none of them were related to tumor-like lesions or squamous cell carcinoma. This is the first report of Avipoxvirus detected in tumor-like lesions of a white-faced whistling duck with phylogenetic analysis of the virus.(AU)

Avipoxvirus é o agente etiológico da varíola (bouba) aviária, uma doença bem descrita em aves de cativeiro e selvagens, tendo sido associada a lesões semelhantes a tumores em algumas dessas espécies. Uma marreca piadeira (Dendrocygna viduata), criada em cativeiro, foi atendida em um Hospital Veterinário na região nordeste devido à presença de nódulos cutâneos em ambas as asas. Alguns dias após o exame clínico, o animal veio a óbito naturalmente. A ave foi submetida à necropsia e coletados fragmentos das lesões para análise histopatológica, que revelou proliferação de células epiteliais expandindo para a derme. Algumas dessas células possuíam material eosinofílico intracitoplasmático e bem definido, sugestivo de inclusão de poxvírus (corpúsculos de Bollinger). A PCR realizada a partir do DNA extraído de amostras das lesões amplificou um fragmento do gene da proteína do núcleo 4b (fpv 167), que foi purificado e sequenciado. Esse fragmento de DNA de Avipoxvirus presente nas lesões relevou alta homologia genética (100,0%) com outros poxvírus detectados em diferentes espécies de aves em vários países, mas nenhum deles estava relacionado a lesões tumorais ou carcinoma espinocelular. Este é o primeiro relato de Avipoxvirus detectado em lesões semelhantes a tumores em uma marreca piadeira com caracterização molecular do vírus.(AU)

Avipoxvirus detected in tumor-like lesions in a white-faced whistling duck (Dendrocygna viduata) / Avipoxvirus detectado em lesões tipo tumorais em uma marreca-irerê (Dendrocygna viduata)

Avipoxvirus is the etiological agent of the avian pox, a well-known disease of captive and wild birds, and it has been associated with tumor-like lesions in some avian species. A white-faced whistling duck (Dendrocygna viduata) raised in captivity was referred to a Veterinary Teaching Hospital in Northeast due to cutaneous nodules present in both wings. A few days after the clinical examination, the animal died naturally. Once submitted to necropsy, histopathological evaluation of the lesions revealed clusters of proliferating epithelial cells expanding toward the dermis. Some of these cells had round, well-defined, intracytoplasmic eosinophilic material suggestive of poxvirus inclusion (Bollinger bodies). PCR performed on the DNA extracted from tissue samples amplified a fragment of the 4b core protein gene (fpv167), which was purified and sequenced. This fragment of Avipoxvirus DNA present in these tumor-like lesions showed high genetic homology (100.0%) with other poxviruses detected in different avian species in several countries, but none of them were related to tumor-like lesions or squamous cell carcinoma. This is the first report of Avipoxvirus detected in tumor-like lesions of a white-faced whistling duck with phylogenetic analysis of the virus.(AU)

Avipoxvirus é o agente etiológico da varíola (bouba) aviária, uma doença bem descrita em aves de cativeiro e selvagens, tendo sido associada a lesões semelhantes a tumores em algumas dessas espécies. Uma marreca piadeira (Dendrocygna viduata), criada em cativeiro, foi atendida em um Hospital Veterinário na região nordeste devido à presença de nódulos cutâneos em ambas as asas. Alguns dias após o exame clínico, o animal veio a óbito naturalmente. A ave foi submetida à necropsia e coletados fragmentos das lesões para análise histopatológica, que revelou proliferação de células epiteliais expandindo para a derme. Algumas dessas células possuíam material eosinofílico intracitoplasmático e bem definido, sugestivo de inclusão de poxvírus (corpúsculos de Bollinger). A PCR realizada a partir do DNA extraído de amostras das lesões amplificou um fragmento do gene da proteína do núcleo 4b (fpv 167), que foi purificado e sequenciado. Esse fragmento de DNA de Avipoxvirus presente nas lesões relevou alta homologia genética (100,0%) com outros poxvírus detectados em diferentes espécies de aves em vários países, mas nenhum deles estava relacionado a lesões tumorais ou carcinoma espinocelular. Este é o primeiro relato de Avipoxvirus detectado em lesões semelhantes a tumores em uma marreca piadeira com caracterização molecular do vírus.(AU)

A retrospective study of neoplasms in ornamental and pet birds diagnosed at the Hospital de Aves of the Universidad Nacional Autónoma de México (2007-2014)

A retrospective study including a total of 44 ornamental and pet birds with neoplasms diagnosed at the Hospital de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México from January 2007 to April 2014 was conducted. Most of affected birds were Psittaciformes (n=26, 59%) including budgerigars (Melopsittacus undulatus) (n=12/26, 46.15%) and amazon parrots (Amazona spp.) (n=7/26, 26.92%), and followed by Passeriformes and Anseriformes (n=7/44, 15.90%, each) and Galliformes (n=4/44, 9.09%). The youngest studied bird was a 6.5-week-old lovebird (Agapornis sp.) with a papilloma, and the oldest bird was a 37-year-old yellow-naped parrot (Amazona auropalliata) with a hemangiosarcoma. Most of the affected birds were adults (n=23, 52.27%). The gender was determined for 17 birds (38.63%), of which eight (47.05%) were females and nine (52.94%) males. There were 45 reported neoplasms,as one salmon-crested cockatoo (Cacatua moluccensis) had two neoplasms. The two most frequent neoplasms were fibrosarcomas (n=9/45, 20%) and hemangiosarcomas (n=5/45, 11.11%). The largest number of neoplasms was found in the integumentary system (n=18/45, 40%), followed by the reproductive system (n=10/45, 22.22%). Of the 16 types of neoplasms, seven (43.75%) were malignant, six (37.5%) were benign, and three (18.75%) were potentially malignant. This study showed similarities with retrospective previously reported studies and contributes to the knowledge of the neoplastic diseases that occur in ornamental and pet birds in Mexico.(AU)

A retrospective study of neoplasms in ornamental and pet birds diagnosed at the Hospital de Aves of the Universidad Nacional Autónoma de México (2007-2014)

A retrospective study including a total of 44 ornamental and pet birds with neoplasms diagnosed at the Hospital de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México from January 2007 to April 2014 was conducted. Most of affected birds were Psittaciformes (n=26, 59%) including budgerigars (Melopsittacus undulatus) (n=12/26, 46.15%) and amazon parrots (Amazona spp.) (n=7/26, 26.92%), and followed by Passeriformes and Anseriformes (n=7/44, 15.90%, each) and Galliformes (n=4/44, 9.09%). The youngest studied bird was a 6.5-week-old lovebird (Agapornis sp.) with a papilloma, and the oldest bird was a 37-year-old yellow-naped parrot (Amazona auropalliata) with a hemangiosarcoma. Most of the affected birds were adults (n=23, 52.27%). The gender was determined for 17 birds (38.63%), of which eight (47.05%) were females and nine (52.94%) males. There were 45 reported neoplasms,as one salmon-crested cockatoo (Cacatua moluccensis) had two neoplasms. The two most frequent neoplasms were fibrosarcomas (n=9/45, 20%) and hemangiosarcomas (n=5/45, 11.11%). The largest number of neoplasms was found in the integumentary system (n=18/45, 40%), followed by the reproductive system (n=10/45, 22.22%). Of the 16 types of neoplasms, seven (43.75%) were malignant, six (37.5%) were benign, and three (18.75%) were potentially malignant. This study showed similarities with retrospective previously reported studies and contributes to the knowledge of the neoplastic diseases that occur in ornamental and pet birds in Mexico.

Occurrence of Avian Mycoplasmas in Free-Living Muscovy-Ducks (Cairina Moschata)

The present study aimed to investigate, by culture and PCR, the occurrence of Mollicutes, Mycoplasma gallisepticum and Mycoplasma synoviae in free-living Muscovy-ducks (Cairina moschata) from the Rio Zoo, RJ, Brazil. Tracheal swabs were obtained from 82 asymptomatic ducks and the samples were submitted to culture of mycoplasmas and PCR for identification of Mollicutes Class, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). Samples were also analyzed directly by PCR, without prior culture, for Mollicutes, MG and MS. Eighteen (18/82) Muscovy-ducks were positive for Mollicutes by culture, all isolates were confirmed as Mollicutes and seven were identified as MG. Of the samples analyzed directly by PCR, without prior culture, 17,1% (14/82) was positive for Mollicutes, being 35,7% (5/14) identified as MG and 21,4% (3/14) as MS. The occurrence of Mollicutes class bacteria was detected in Muscovy-ducks. MG and MS were identified in these animals suggesting the circulation of these agents in the Rio de Janeiro Zoo and may present a risk for the health status of the other birds.(AU)

Occurrence of Avian Mycoplasmas in Free-Living Muscovy-Ducks (Cairina Moschata)

The present study aimed to investigate, by culture and PCR, the occurrence of Mollicutes, Mycoplasma gallisepticum and Mycoplasma synoviae in free-living Muscovy-ducks (Cairina moschata) from the Rio Zoo, RJ, Brazil. Tracheal swabs were obtained from 82 asymptomatic ducks and the samples were submitted to culture of mycoplasmas and PCR for identification of Mollicutes Class, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). Samples were also analyzed directly by PCR, without prior culture, for Mollicutes, MG and MS. Eighteen (18/82) Muscovy-ducks were positive for Mollicutes by culture, all isolates were confirmed as Mollicutes and seven were identified as MG. Of the samples analyzed directly by PCR, without prior culture, 17,1% (14/82) was positive for Mollicutes, being 35,7% (5/14) identified as MG and 21,4% (3/14) as MS. The occurrence of Mollicutes class bacteria was detected in Muscovy-ducks. MG and MS were identified in these animals suggesting the circulation of these agents in the Rio de Janeiro Zoo and may present a risk for the health status of the other birds.

Gastrointestinal helminths and related histopathological lesions in black-necked swans Cygnus melancoryphus from the Carlos Anwandter Nature Sanctuary, Southern Chile / Helmintos gastrintestinais e lesões histopatológicas relacionadas em cisnes de pescoço negro Cygnus melancoryphus do Santuário da Natureza Carlos Anwandter, Sul do Chile

Black-necked swans are distributed across South America and face conservation problems in Chile according to data of the State institution SAG. The aim of this study was to identify helminths and to assess associated tissue damage via histopathology. A total of 19,291 parasites were isolated from 21 examined birds; 17 species were identified, including nematodes, flukes, and tapeworms. Of these, 12 were new host records, 13 were reported for the first time in Chile, and 5 were new records for the Neotropical region. Further, the flukes Schistosomatidae gen. sp. and Echinostoma echinatum are of zoonotic concern. Regarding histopathology, an inflammatory response was found along the birds entire digestive tract. Nevertheless, it is difficult to declare that there is a clear association between such lesions and isolated parasites, as other noxa could be responsible as well. Although in some cases there was an evident association, such inflammatory responses and necrosis were minimal, as occurred with Capillaria, Retinometra, Catatropis, Echinostoma, and Schistosomatidae gen. sp. Nevertheless, Epomidiostomum vogelsangi caused granulomatous injuries, an important inflammatory response, and necrosis, but it always circumscribed to superficial layers of the gizzard. Conversely, Paramonostomum was not associated with an inflammatory response despite a high parasitic load.(AU)

O cisne de pescoço negro é distribuído por toda a América do Sul, e enfrenta problemas de conservação no Chile, sendo protegido pela Lei Estadual de Caça. O objetivo deste estudo foi identificar helmintos em cisnes e avaliar o dano tecidual por meio de histopatologia. Um total de 19.291 parasitas foi isolado de 21 aves examinadas, sendo 17 espécies identificadas, entre nematóides, trematódeos e tênias. Destes, 12 são novos registros de hospedeiros, 13 são reportados pela primeira vez no Chile, e 5 são novos registros para a região Neotropical. Além disso, os trematódeos Schistosomatidae gen. sp. e Echinostoma echinatum detectados têm importância zoonótica. Em relação à histopatologia, uma resposta inflamatória foi encontrada em todo o trato digestivo. Entretanto, é difícil estabelecer uma associação estrita de tais lesões com parasitas isolados, porque outros fatores também poderiam ser responsáveis. Em alguns casos, houve uma associação óbvia entre parasitas e lesões, embora a resposta inflamatória e a necrose fossem mínimas, como foi o caso dos gêneros Capillaria, Retinometra, Catatropis, Echinostoma e Schistosomatidae gen. sp. Entretanto, Epomidiostomum vogelsangi causou lesões granulomatosas com importante resposta inflamatória e necrose, mas sempre circunscrita às camadas superficiais da moela. Por outro lado, Paramonostomum não foi associado com uma resposta inflamatória óbvia apesar da alta carga parasitária.(AU)

Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil / Detecção de metapneumovirus aviário subtipo A em aves silvestres no estado de São Paulo, Brasil

The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection.(AU)

O presente estudo investigou a circulação de metapneumovírus aviário em aves silvestres no Brasil. Para tanto, 131 amostras de 366 suabes orofaringeanos ou cloacais coletados de 18 espécies de aves foram testadas individualmente ou na forma de pools por RT-PCR. As amostras detectadas por RT-PCR foram selecionadas para sequenciamento. Treze (9,9%) das amostras foram detectadas por RT-PCR tendo o gene N como alvo; destas, quatro foram sequenciadas com sucesso. Resultados do sequenciamento mostraram alta identidade com o aMPV de subtipo A. Nossos resultados confirmam a circulação de aMPV subtipo A em aves silvestres no Brasil mesmo cinco anos após sua última detecção.(AU)

Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil / Detecção de metapneumovirus aviário subtipo A em aves silvestres no estado de São Paulo, Brasil

The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection.(AU)

O presente estudo investigou a circulação de metapneumovírus aviário em aves silvestres no Brasil. Para tanto, 131 amostras de 366 suabes orofaringeanos ou cloacais coletados de 18 espécies de aves foram testadas individualmente ou na forma de pools por RT-PCR. As amostras detectadas por RT-PCR foram selecionadas para sequenciamento. Treze (9,9%) das amostras foram detectadas por RT-PCR tendo o gene N como alvo; destas, quatro foram sequenciadas com sucesso. Resultados do sequenciamento mostraram alta identidade com o aMPV de subtipo A. Nossos resultados confirmam a circulação de aMPV subtipo A em aves silvestres no Brasil mesmo cinco anos após sua última detecção.(AU)

Serological evidence of exposure to Toxoplasma gondii and Neospora caninum in free-ranging Orinoco goose (Neochen jubata) in Brazil / Evidência sorológica de exposição à Toxoplasma gondii e Neospora caninum em Ganso-do-Orinoco (Neochen jubata) de vida livre no Brasil

Toxoplasma gondii and Neospora caninum are Apicomplexan intracellular protozoan parasites that affect numerous animal species, thus leading to severe diseases and economic losses, depending on the vertebrate species involved. The role of the avian species in maintaining and transmission of these coccidia has been studied for several years as they tend to serve as a potential source of infection for mammals and humans. The present study aimed to assess the serological exposure of Orinoco goose (Neochen jubata) to T. gondii and N. caninum. Between 2010 and 2013, 41 free-ranging Orinoco geese were captured in the Araguaia River, Brazil. The presence and titration of IgY antibodies to both coccidia were assayed via indirect immunofluorescent antibody test (IFAT). While IgY antibodies for N. caninum were present in 5 animals, with titers of 20, the antibodies for T. gondii were found in 35 animals, with titers ranging from 20 to 640. Considering that the Orinoco gooses meat is consumed by the local population in the studied area, it may represent an important source of T. gondii infection for humans. Due to its migratory behavior, this goose may play a pivotal role in the natural dispersion of both parasites. Furthermore, molecular studies are required for genotyping the isolates of T. gondii that occurs in this avian species.(AU)

Toxoplasma gondii e Neospora caninum são parasitas protozoários intracelulares do philo Aplicomplexa que afetam uma vasta gama de espécies animais, causando sérias doenças e levando a perdas econômicas, dependendo da espécie envolvida. O papel das aves na manutenção e transmissão destes coccídios tem sido estudado por anos, já que eles são potenciais fontes de infecção para outros animais e humanos. O objetivo deste estudo foi avaliar a exposição do Ganso-do-Orinoco (Neochen jubata) a T. gondii e N. caninum por meio de técnicas sorológicas. Entre os anos de 2010 e 2013, 41 Gansos-do-Orinoco de vida livre foram capturados no Vale do Rio Araguaia, Brasil. A presença e titulação de anticorpos IgY para ambos os coccídios foi obtida utilizando-se a Reação de Imunofluorescência Indireta (RIFI). Enquanto a presença de anticorpos IgY para N. caninum foi detectada em 5 aves, com titulação 20, anticorpos para T. gondii foram encontrados em 35 aves, com títulos variando de 20 a 640. Considerando que a carne do Ganso-do-Orinoco é uma fonte de alimento para a população da área estudada, a ave pode representar uma importante fonte de infecção de T. gondii para humanos. Devido ao seu comportamento migratório, esta espécie assume grande importância na dispersão de ambos os parasitas. Estudos moleculares são necessários a fim de caracterizar genotipicamente os isolados de T. gondii que ocorrem nesta espécie de ave.(AU)

Serological diagnosis of influenza A subtype H1 on family poultry of Belo Horizonte, Minas Gerais and Santa Maria, Rio Grande Do Sul, in Brazil

Serum samples (n=687) from Gallus gallus domesticus were collected for the investigation of antibodies to avian influenza virus (AIV-A) in the family poultry of the surrounding counties of Santa Maria/RS and the metropolitan region of Belo Horizonte/MG, totaling twenty different counties. Additional samples of seventeen (n=17) free-flying ducks (C. moschata pure or hybrid with Anas platyrhynchos) were collected in Belo Horizonte. The chosen tests for the survey were performed as described by the World Organization for Animal Health (OIE), including agar gel immunodiffusion (AGID) for antibodies to AIV-A nucleoprotein (N) and haemagglutination- inhibition (HI) for antibodies to subtype H1. Out of the 704 serum tests performed by AGID, eight (8/704) were revealed positive for antibodies to AIV-A N protein, with six (6/704) retested positive for subtype H1. Two sera tested positive by AGID were shown to be non reactive to the H1 subtype, suggesting specificity to another subtype. A low occurrence of antibodies to influenza A (1.13%) was found, and mostly (75%) specific to subtype H1. This represents an approximately 0,85% overall occurrence for subtype H1 antibodies, with an unknown subtype specific antibodies detected in one free-flying anatid. The low occurrence of antibodies in the family poultry may suggest a low AIV-A activity during the period of study, information which remains to be confirmed by virus detection.(AU)

Serological diagnosis of influenza A subtype H1 on family poultry of Belo Horizonte, Minas Gerais and Santa Maria, Rio Grande Do Sul, in Brazil

Serum samples (n=687) from Gallus gallus domesticus were collected for the investigation of antibodies to avian influenza virus (AIV-A) in the family poultry of the surrounding counties of Santa Maria/RS and the metropolitan region of Belo Horizonte/MG, totaling twenty different counties. Additional samples of seventeen (n=17) free-flying ducks (C. moschata pure or hybrid with Anas platyrhynchos) were collected in Belo Horizonte. The chosen tests for the survey were performed as described by the World Organization for Animal Health (OIE), including agar gel immunodiffusion (AGID) for antibodies to AIV-A nucleoprotein (N) and haemagglutination- inhibition (HI) for antibodies to subtype H1. Out of the 704 serum tests performed by AGID, eight (8/704) were revealed positive for antibodies to AIV-A N protein, with six (6/704) retested positive for subtype H1. Two sera tested positive by AGID were shown to be non reactive to the H1 subtype, suggesting specificity to another subtype. A low occurrence of antibodies to influenza A (1.13%) was found, and mostly (75%) specific to subtype H1. This represents an approximately 0,85% overall occurrence for subtype H1 antibodies, with an unknown subtype specific antibodies detected in one free-flying anatid. The low occurrence of antibodies in the family poultry may suggest a low AIV-A activity during the period of study, information which remains to be confirmed by virus detection.

Pododermite em cisne negro (Cygnus atratus) em Moçambique / Bumblefoot in free-living black swan (Cygnus atratus) in Mozambique

Background: Black Swan (Cygnus atratus Linnaeus 1766) is an aquatic bird native to Australia and non-migratory birds. Bumblefoot is a chronic, progressive, degenerative and granulomatous disease that affects the plantar region and causes inflammation and degenerative changes in plantar tissues. Given that bumblefoot incidence in free-living wild birds is low and that the literature lacks publications about bumblefoot in free-living Black Swans, the aim of the current study is to describe the first bumblefoot case reported in a free-living Black Swan (Cygnus atratus) in Mozambique who was subjected to surgical procedure.Case: An adult intact Black Swan (Cygnus atratus) weighing 7.2 kg was presented to Veterinary Hospital of the Veterinary Medicine School, Mozambique with reluctance to support the left hind limb. Water intake was not unsettled and urine and feces were normal. A brown mass with 3 cm diameter approximately was identified in the footpad of the left hind limb; there were local bleeding and ulcers and pain. Footpad radiographic images of the left hind limb showed no abnormalities in the bone tissue. An incisional biopsy was performed with patient under sedation. The cytopathological examination was suggestive of bumblefoot. An excisional biopsy was performed with patient under anesthesia. The mass was excised with surgical margin of 1 cm and the local was subjected to debridement. The sample was sent to histopathological examination. The histopathological examination confirmed a chronic Bumblefoot Type 2. The patient started to support the left hind limb three days after surgery and twenty days after surgery, the footpad sutures were removed. Fifty days after the surgical procedure the patient was released in the site it was rescued.[...]

Pododermite em cisne negro (Cygnus atratus) em Moçambique / Bumblefoot in free-living black swan (Cygnus atratus) in Mozambique

Background: Black Swan (Cygnus atratus Linnaeus 1766) is an aquatic bird native to Australia and non-migratory birds. Bumblefoot is a chronic, progressive, degenerative and granulomatous disease that affects the plantar region and causes inflammation and degenerative changes in plantar tissues. Given that bumblefoot incidence in free-living wild birds is low and that the literature lacks publications about bumblefoot in free-living Black Swans, the aim of the current study is to describe the first bumblefoot case reported in a free-living Black Swan (Cygnus atratus) in Mozambique who was subjected to surgical procedure.Case: An adult intact Black Swan (Cygnus atratus) weighing 7.2 kg was presented to Veterinary Hospital of the Veterinary Medicine School, Mozambique with reluctance to support the left hind limb. Water intake was not unsettled and urine and feces were normal. A brown mass with 3 cm diameter approximately was identified in the footpad of the left hind limb; there were local bleeding and ulcers and pain. Footpad radiographic images of the left hind limb showed no abnormalities in the bone tissue. An incisional biopsy was performed with patient under sedation. The cytopathological examination was suggestive of bumblefoot. An excisional biopsy was performed with patient under anesthesia. The mass was excised with surgical margin of 1 cm and the local was subjected to debridement. The sample was sent to histopathological examination. The histopathological examination confirmed a chronic Bumblefoot Type 2. The patient started to support the left hind limb three days after surgery and twenty days after surgery, the footpad sutures were removed. Fifty days after the surgical procedure the patient was released in the site it was rescued.[...](AU)

Occurrence of Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae), a nasal mite in fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in Southern Brazil / Ocorrência de Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) na região sul do Rio Grande do Sul, Brasil

The authors report the occurrence of the nasal mite Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) in free-living fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in the Rio Grande do Sul region, Southern Brazil. Prevalence and mean intensity showed values of 35.5% and 2.27 mites to D. bicolor and 13% and 9.33 mites for D. viduatta. The intensity of parasitism ranged from 1 to 6 mites in D. bicolor, and 1 to 22 mites in D. viduatta. This is the first report of R. rhinolethrum on D. bicolor and D. viduata in Rio Grande do Sul, expanding the list of anatids parasitized by this species in this State.

Os autores relatam a ocorrência do ácaro nasal Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) de vida livre na região sul do Rio Grande do Sul. Para a prevalência e intensidade média foram observados 35,5% e 2,27 ácaros para D. bicolor e 13% e 9,33 ácaros para D. viduatta. A intensidade de parasitismo observada variou de 1 a 6 ácaros em D. bicolor e 1 a 22 ácaros em D. viduatta. O parasitismo caracteriza o primeiro relato da ocorrência de R. rhinolethrum sobre Dendrocygna bicolor e sobre Dendrocygna viduata no Rio Grande do Sul, ampliando a listagem dos anatídeos parasitados por essa espécie neste Estado.

Ocorrência de Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) na região sul do Rio Grande do Sul, Brasil / Occurrence of Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae), a nasal mite in fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in Southern Brazil

Os autores relatam a ocorrência do ácaro nasal Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) de vida livre na região sul do Rio Grande do Sul. Para a prevalência e intensidade média foram observados 35,5% e 2,27 ácaros para D. bicolor e 13% e 9,33 ácaros para D. viduatta . A intensidade de parasitismo observada variou de 1 a 6 ácaros em D. bicolor e 1 a 22 ácaros em D. viduatta . O parasitismo caracteriza o primeiro relato da ocorrência de R. rhinolethrum sobre Dendrocygna bicolor e sobre Dendrocygna viduata no Rio Grande do Sul, ampliando a listagem dos anatídeos parasitados por essa espécie neste Estado.(AU)

The authors report the occurrence of the nasal mite Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) in free-living fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in the Rio Grande do Sul region, Southern Brazil. Prevalence and mean intensity showed values of 35.5% and 2.27 mites to D. bicolor and 13% and 9.33 mites for D. viduatta . The intensity of parasitism ranged from 1 to 6 mites in D. bicolor , and 1 to 22 mites in D. viduatta . This is the first report of R. rhinolethrum on D. bicolor and D. viduata in Rio Grande do Sul, expanding the list of anatids parasitized by this species in this State.(AU)

Occurrence of Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae), a nasal mite in fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in Southern Brazil / Ocorrência de Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) na região sul do Rio Grande do Sul, Brasil

The authors report the occurrence of the nasal mite Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) in free-living fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) in the Rio Grande do Sul region, Southern Brazil. Prevalence and mean intensity showed values of 35.5% and 2.27 mites to D. bicolor and 13% and 9.33 mites for D. viduatta. The intensity of parasitism ranged from 1 to 6 mites in D. bicolor, and 1 to 22 mites in D. viduatta. This is the first report of R. rhinolethrum on D. bicolor and D. viduata in Rio Grande do Sul, expanding the list of anatids parasitized by this species in this State.(AU)

Os autores relatam a ocorrência do ácaro nasal Rhinonyssus rhinolethrum (Trouessart, 1895) (Acarina: Rhinonyssidae) em marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) de vida livre na região sul do Rio Grande do Sul. Para a prevalência e intensidade média foram observados 35,5% e 2,27 ácaros para D. bicolor e 13% e 9,33 ácaros para D. viduatta. A intensidade de parasitismo observada variou de 1 a 6 ácaros em D. bicolor e 1 a 22 ácaros em D. viduatta. O parasitismo caracteriza o primeiro relato da ocorrência de R. rhinolethrum sobre Dendrocygna bicolor e sobre Dendrocygna viduata no Rio Grande do Sul, ampliando a listagem dos anatídeos parasitados por essa espécie neste Estado.(AU)

Chewing lice (Insecta, Phthiraptera) of the fulvous whistling duck Dendrocygna bicolor (Vieillot, 1816) and white-faced whistling-duck Dendrocygna viduata (Linnaeus, 1766) from Rio Grande do Sul Southern Brazil / Malófagos (Insecta, Phthiraptera) de marreca caneleira Dendrocygna bicolor (Vieillot, 1816) e marreca piadeira Dendrocygna viduata (Linnaeus, 1766) na região sul do Rio Grande do Sul

The authors report the occurrence of Acidoproctus rostratus (Rudow, 1866), Holomenopon leucoxanthum (Burmeister, 1838), Trinoton aculeatum (Piaget, 1885), Trinoton querquedulae (Linnaeus, 1758) and Anatoecus dentatus (Scopoli, 1763) in Dendrocygna bicolor (Vieillot, 1816) and Dendrocygna viduata (Linnaeus, 1766); Anatoecus icterodes (Nitzsch, 1818) in D. bicolor and Anaticola chaetodens (Eichler, 1954) in D. viduata. Significant differences in the prevalence were found between T. aculeatum and T. querquedulae in D. bicolor as well as D. viduata, which is characterized as a potential event of colonization. Significant differences were also found for the prevalence and mean abundance of Anatoecus spp. between D. bicolor and D. viduata. The occurrence of Trinoton querquedulae in D. bicolor and D. viduata, of Anatoecus dentatus in D. bicolor and Anaticola chaetodens in D. viduata characterize new hosts species to these species of lice.

Os autores relatam a ocorrência de Acidoproctus rostratus (Rudow, 1866), Holomenopon leucoxanthum (Burmeister, 1838), Trinoton aculeatum (Piaget, 1885), Trinoton querquedulae (Linnaeus, 1758) e Anatoecus dentatus (Scopoli, 1763) em Dendrocygna bicolor (Vieillot, 1816) e Dendrocygna viduata (Linnaeus, 1766); Anatoecus icterodes (Nitzsch, 1818) em D. bicolor e Anaticola chaetodens (Eichler, 1954) em D. viduata. Diferenças significativas na prevalência foram encontradas entre T. aculeatum e T. querquedulae, tanto em D. bicolor quanto em D. viduata, sendo esse achado caracterizado como um evento potencial de colonização. Outras diferenças também foram encontradas para a prevalência e a abundância média de Anatoecus spp. entre D. bicolor e D. viduata. A ocorrência de Trinoton querquedulae em D. bicolor e D. viduata, de Anatoecus dentatus em D. bicolor e de Anaticola chaetodens em D. viduata, caracteriza novos hospedeiros para essas espécies de piolhos.