Survey of molds, yeast and Alicyclobacillus spp. from a concentrated apple juice productive process

Bacteria and molds may spoil and/or contaminate apple juice either by direct microbial action or indirectly by the uptake of metabolites as off-flavours and toxins. Some of these microorganisms and/or metabolites may remain in the food even after extensive procedures. This study aim to identify the presence of molds (including heat resistant species) and Alicyclobacillus spp., during concentrated apple juice processing. Molds were isolated at different steps and then identified by their macroscopic and microscopic characteristics after cultivation on standard media at 5, 25 and 37ºC, during 7 days. Among the 19 isolated found, 63% were identified as Penicillium with 50% belonging to the P. expansum specie. With regards to heat resistant molds, the species Neosartorya fischeri, Byssochlamys fulva and also the genus Eupenicillium sp., Talaromyces sp. and Eurotium sp. were isolated. The thermoacidophilic spore-forming bacteria were identified as A. acidoterrestris by a further investigation based on 16S rRNA sequence similarity. The large contamination found indicates the need for methods to eliminate or prevent the presence of these microorganisms in the processing plants in order to avoid both spoilage of apple juice and toxin production.(AU)

Avaliação microbiológica do caldo de cana comercializado por ambulantes e estabelecimentos formais em Salvador, BA

O objetivo desse estudo foi avaliar a qualidade microbiológica do caldo de cana comercializado por ambulantes e estabelecimentos formais em Salvador-Bahia e comparar com padrões sanitários vigentes na legislação. Trata-se de um estudo transversal, onde foram realizadas análises microbiológicas para determinação de coliformes totais, termotolerantes, Salmonella spp, e bolores e leveduras, e das condições higienicossanitárias dos locais de comercialização do produto por meio da aplicação de uma lista de checagem. Os resultados obtidos foram comparados com os padrões sanitários determinados pela legislação brasileira onde, tanto os estabelecimentos formais quanto os informais, apresentaram um nível considerável de contaminação, com apenas uma amostra satisfatória. Pode-se verificar que o caldo de cana ofertado, tanto pelo setor formal quanto pelo informal, estava impróprio para consumo, pois a análise microbiológica mostrou que a maioria das amostras apresentavam contaminação acima do limite aceitável da legislação, além da presença de micro-organismos indicadores de qualidade higienicassanitária insatisfatória.(AU)

The objective of this study was to evaluate the microbiological quality of sugarcane juice marketed by street vendors and formal establishments in Salvador - Bahia and compare it with sanitary standards in force in the legislation. This is a cross-sectional study where microbiological analyzes were carried out to determine the total and thermotolerant coliforms, Salmonella spp, molds and yeasts and the hygienic sanitary conditions of the product commercialization sites through the application of a checklist. The results obtained were compared to sanitary standards determined by Brazilian legislation, where both formal and informal establishments presented a considerable level of contamination, with only a satisfactory sample. It can be verified that the sugarcane juice offered by both the formal and the informal sectors were unfit for consumption, since the microbiological analysis showed that the majority of the samples were above the acceptable limit of the legislation, besides the presence of microorganisms indicating poor hygienic quality sanitary conditions.(AU)

Análise microbiológica do guaraná energético da Amazônia, comercializado em São Luís, MA / Microbiological analysis of the energy guarana Amazon marketed in São Luis, MA

Foram avaliadas as condições microbiológicas da bebida mista não alcoólica à base de guaraná (Paullinia cupana), conhecida popularmente com "Guaraná da Amazônia", considerada como energética e, sendo produzida artesanalmente, é comercializada em sua maioria de maneira imprópria em São Luís/MA. O presente estudo objetivou analisar a qualidade dos micro-organismos contaminantes e potencialmente patogênicos que pode colocar em risco a saúde da população. As amostras foram analisadas no Laboratório de Microbiologia Médica - UniCeuma. As mesmas foram diluídas em solução de cloreto de sódio estéril e submetidas à técnisa do~ Tub~~ Múltiplos, seriados em 10 , 10 e 10 ,com caldo Lactosado Simples, caldo Verde Brilhante para coliformes totais e, para a análise de termotolerantes, o caldo EC em banho-maria à temperatura de 44,SoC e caldo Rapapport para o isolamento de espécies de Salmonella. As amostras com indicativo de serem positivas em caldo foram depois semeadas em placas de Petri contendo ágar Salmonella e Shigella, MacConkey, Saboround e Manitol. Baseado nos parâmetros de tolerância da Agência Nacional de Vigilância Sanitária, pode-se constatar que as amostras analisadas encontram- -se fora dos padrões determinados pela legislação vigente tomando esse produto inadequado para o consumo, necessitando de uma maior atenção dos órgãos competentes. (AU)

The evaluation of the microbiological conditions of non-alcoholic mixed drink based on guarana (Paullinia cupana), known popularly as "guarana amazon ", was done. This product is considered as energy and; handcrafted, is marketed mostly improperly in São Luís/MA. This present study aimed to analyze the quality of micro-organisms and potentially pathogenic contaminants that may endanger public health. The samples were analyzed at the laboratory of medical microbiology - Uni- Ceuma. The samples were diluted in sterile sodium chloride and subjected to the Tecniche of Multiple Tubes, in series 10 , 10 and 10 , with lactose broth simple, brilliant green broth for total coliforms, and for analysis of coliforms, the EC's broth in a water bath at a temperature of 44.5°C and Rapapport's broth for the isolation of salmonella species. The samples that indicate positives in broth were then seeded in. Petri's plates containing agar Salmonella and Shigella, Mac-Conkey, Saboround and Mannitol. Based on the parameters of tolerance of Brazilian Sanitary Surveillance Bureau can be seen that the samples are outside of standards set by legislation making this product unsuitable for consumption, requiring greater attention of the competent bodies. (AU)