Comparative study of biotin supplementation on weight gain and occurrence of digital diseases in cattle (Bos taurus x Bos indicus) / Estudo comparativo da suplementação com biotina sobre o ganho de peso e ocorrência de enfermidades digitais em bovinos (Bos taurus x Bos indicus)

This study aimed to assess the weight gain and the incidence of foot diseases in male, crossbred (Bos taurus x Bos indicus) bovines that were supplemented with biotin. An amount of 240 animals, supplemented or not with biotin, allocated in 12 groups of 20 animals, was assessed for a period of six months. The study was conducted during three years and the groups were divided according to the forage available, corn, corn residue and sorghum silage, and initial weights between 100 and 200 kg and between 200 and 300 kg. The statistical analyses used were the Tukeys Test, in triple factorial scheme (type of silage x use of biotin x initial body weight) and the Fisher´s Exact Test, both at 5% significance level. The biotin supplementation in bovines did not influence weight gain and the incidence of foot diseases, however, when comparing only the type of forage, corn and sorghum silage provided higher weight gain than silage made of corn residue.(AU)

Esse estudo teve como objetivo avaliar o ganho em peso e a ocorrência de enfermidades digitais em bovinos do sexo masculino, mestiços (Bos taurus x Bos indicus) que foram suplementados com biotina. Avaliaram-se, por um período de seis meses, 240 bovinos, suplementados ou não com biotina, alocados em 12 grupos de 20 animais. O estudo foi realizado durante três anos e os grupos foram divididos de acordo com o volumoso disponibilizado, silagem de milho, de resíduo de milho e de sorgo, e pesos iniciais entre 100 e 200 kg e entre 200 e 300 kg. As análises estatísticas empregadas foram o Teste de Tukey, em esquema de fatorial triplo (tipo de silagem x uso da biotina x peso corporal inicial) e o Teste Exato de Fisher, ambas em nível de significância de 5%. A suplementação com biotina nos bovinos não exerceu influência sobre o ganho em peso e a ocorrência de enfermidades digitais, mas, quando se comparou apenas o tipo de volumoso, a silagem de milho e sorgo, pode se observar um maior ganho em peso que a silagem confeccionada de resíduo de milho.(AU)

The effect of topical application of Stryphnodendron adstringens (Martius) Coville extract and oral biotin supplementation on recovery of wounds from digital dermatitis surgery / Aplicação tópica do extrato da casca do barbatimão Stryphnodendron adstringens (Martius) Coville associada à administração oral de biotina na reparação de feridas cirúrgicas de dermatite digital bovina

Digital dermatitis can impair the health and milk yield of dairy cattle. Treatment of digital dermatitis involves corrective trimming and footbaths. The results of these are not always satisfactory, with na added risk of persisting residues in the dairy products and environment. In this study, we evaluated the effect of Stryphnodendron adstringens (Martius) Coville extract (applied via footbath or directly over the lesions) and biotin supplementation on wound healing in 90 surgically-corrected digital dermatitisaffected dairy cows. All animals were female, crossbred (Holstein × Gir), affected with digital dermatitis to different degrees of severity. The lesions were evaluated over a 45-day post-surgical period, and the cows were divided to six treatment groups. The groups were divided based on application of the extract as a footbath solution with or without supplemental biotin, application by brushing with or without biotin, and cleaning with water with or without biotin. The evaluation was performed considering the rates and recovery times. Supplementation with biotin did not influence the rate or recovery time of the surgical wounds. Topical application of the bark extract (via footbath solution and brushing) was determined to be more beneficial than washing with water.(AU)

A dermatite digital compromete a saúde e produtividade dos bovinos. O controle da enfermidade pode ser feito com a apara corretiva do casco e passagem dos animais em pedilúvio. Os resultados nem sempre são satisfatórios e há o risco de ocorrerem resíduos na carne, leite e no ambiente. No presente estudo avaliou-se a reparação de feridas cirúrgicas de dermatite digital após aplicação tópica do extrato da casca de barbatimão, em pedilúvio ou por pincelamento, e suplementação com biotina, em 90 vacas de aptidão leiteira. Todos os animais eram fêmeas, mestiças (Gir x Holandesa) e apresentavam dermatite digital em graus variados. As feridas foram analisadas em vários momentos durante 45 dias. Os animais foram divididos em seis grupos de 15 de acordo com o protocolo. Avaliou-se a aplicação do barbatimão em pedilúvio com ou sem suplementação de biotina, barbatimão por pincelamento com ou sem biotina e limpeza com água sob pressão com ou sem biotina, considerando os índices e os tempos de recuperação. A suplementação com biotina não influenciou os índices nem o tempo de recuperação das feridas cirúrgicas de dermatite digital e o uso tópico do extrato da casca do barbatimão, independente da forma de aplicação, se mostrou benéfico em relação à aplicação de água.(AU)

The effect of topical application of Stryphnodendron adstringens (Martius) Coville extract and oral biotin supplementation on recovery of wounds from digital dermatitis surgery / Aplicação tópica do extrato da casca do barbatimão Stryphnodendron adstringens (Martius) Coville associada à administração oral de biotina na reparação de feridas cirúrgicas de dermatite digital bovina

Digital dermatitis can impair the health and milk yield of dairy cattle. Treatment of digital dermatitis involves corrective trimming and footbaths. The results of these are not always satisfactory, with na added risk of persisting residues in the dairy products and environment. In this study, we evaluated the effect of Stryphnodendron adstringens (Martius) Coville extract (applied via footbath or directly over the lesions) and biotin supplementation on wound healing in 90 surgically-corrected digital dermatitisaffected dairy cows. All animals were female, crossbred (Holstein × Gir), affected with digital dermatitis to different degrees of severity. The lesions were evaluated over a 45-day post-surgical period, and the cows were divided to six treatment groups. The groups were divided based on application of the extract as a footbath solution with or without supplemental biotin, application by brushing with or without biotin, and cleaning with water with or without biotin. The evaluation was performed considering the rates and recovery times. Supplementation with biotin did not influence the rate or recovery time of the surgical wounds. Topical application of the bark extract (via footbath solution and brushing) was determined to be more beneficial than washing with water.

A dermatite digital compromete a saúde e produtividade dos bovinos. O controle da enfermidade pode ser feito com a apara corretiva do casco e passagem dos animais em pedilúvio. Os resultados nem sempre são satisfatórios e há o risco de ocorrerem resíduos na carne, leite e no ambiente. No presente estudo avaliou-se a reparação de feridas cirúrgicas de dermatite digital após aplicação tópica do extrato da casca de barbatimão, em pedilúvio ou por pincelamento, e suplementação com biotina, em 90 vacas de aptidão leiteira. Todos os animais eram fêmeas, mestiças (Gir x Holandesa) e apresentavam dermatite digital em graus variados. As feridas foram analisadas em vários momentos durante 45 dias. Os animais foram divididos em seis grupos de 15 de acordo com o protocolo. Avaliou-se a aplicação do barbatimão em pedilúvio com ou sem suplementação de biotina, barbatimão por pincelamento com ou sem biotina e limpeza com água sob pressão com ou sem biotina, considerando os índices e os tempos de recuperação. A suplementação com biotina não influenciou os índices nem o tempo de recuperação das feridas cirúrgicas de dermatite digital e o uso tópico do extrato da casca do barbatimão, independente da forma de aplicação, se mostrou benéfico em relação à aplicação de água.

Carcinossarcoma mamário em uma gata: caracterização anatomopatológica e imuno-histoquímica / Mammary carcinosarcoma in a cat: anatomopathological and immunohistochemical characterization

Background : Mammary tumor is uncommon in cats. In felines is most frequently observed in old animals and Siamese breed. Apparently there is no relation between the age of the animal at the time of spay how is observed in dogs, although the progression from a focal hyperplasia to an adenoma or a carcinoma is known. The ratio between malignant and benign tumors is estimated between 9:1 and 4:1, most of them are adenocarcinomas and highly metastatic behavior. Mammary carcinosarcoma is rare in felines. The objective of this work was to describe a case of carcinosarcoma in a female cat. Case : Mammary gland mass from a 13-year-old, intact female Siamese cat was submitted for biopsy. The tumor was observed recently by the owners, who reported that the animal never received contraceptives. Macroscopically, it was observed focally extensive poorly demarcated, whitened, firm mass measuring 4.0 x 3.0 x 1.5 cm, recovered by skin with central round area of ulceration measuring 2.0 cm in diameter. Histologically, the neoplasm was composed of epithelial cells and spindle mesenquimal cells with bone and cartilaginous differentiation. Immunohistochemistry for cytokeratin AE1/AE3 and 8/18 were stained intracitoplasmatic positive, accentuated (+++) and moderate (++), respectively in tubular epithelia in more than 50% of the cells. There was immunostaining for vimentin in the cytoplasm of mesenchymal neoplastic cells, cartilaginous and tubular epithelial cells with moderate intensity (++) in less than 50% of the cells. Discussion: Carcinosarcoma is a rare neoplasm in cats and have being reported in the mammary gland and in the lungs. The neoplasm is characterized by mesenchymal and epithelial cells. In many cases the histological exam is insufficient to determinate the diagnosis, being necessary to do the immunohistochemistry (IHC) exam to verify vimentin and cytokeratin coexpression in neoplasic cells. Histologically, was observed epithelial and mesenchymal characteristics, confi rmed by IHC. In another paper, carcinosarcoma demonstrated similar arranged, as heterogenic cellular composition and infiltrative proliferation of mesenchymal cells. In this case report was also observed cartilaginous and osseous differentiation. Some carcinosarcoma can present differentiated mesenchymal tissue and a mixture of all carcinomatosos and sarcomatosos elements in the same neoplasic mass. Histogenesis of carcinosarcoma remains uncertain thus far, although two theories suggest its origin: a multiclonal and other monoclonal. The first one suggests that mesenchymal and epithelial cells are originated from two or more different base cells. The second theory suggests that the tumors behavior is the result of a totipotent ability of neoplasic cells to differ and be histologically recognizable as epithelial and mesenchymal structures. In this study, tubular epithelial cells were positive stained for cytokeratins (AE1/AE3 and 8/18) and to vimentin, which suggest a monoclonal origin, because it was observed epithelial and mesenchymal differentiation. According to localization, morphology features and immunohistochemistry evaluation, the present study reports a rare case of carcinosarcoma in a cat.(AU)

Carcinossarcoma mamário em uma gata: caracterização anatomopatológica e imuno-histoquímica / Mammary carcinosarcoma in a cat: anatomopathological and immunohistochemical characterization

Background : Mammary tumor is uncommon in cats. In felines is most frequently observed in old animals and Siamese breed. Apparently there is no relation between the age of the animal at the time of spay how is observed in dogs, although the progression from a focal hyperplasia to an adenoma or a carcinoma is known. The ratio between malignant and benign tumors is estimated between 9:1 and 4:1, most of them are adenocarcinomas and highly metastatic behavior. Mammary carcinosarcoma is rare in felines. The objective of this work was to describe a case of carcinosarcoma in a female cat. Case : Mammary gland mass from a 13-year-old, intact female Siamese cat was submitted for biopsy. The tumor was observed recently by the owners, who reported that the animal never received contraceptives. Macroscopically, it was observed focally extensive poorly demarcated, whitened, firm mass measuring 4.0 x 3.0 x 1.5 cm, recovered by skin with central round area of ulceration measuring 2.0 cm in diameter. Histologically, the neoplasm was composed of epithelial cells and spindle mesenquimal cells with bone and cartilaginous differentiation. Immunohistochemistry for cytokeratin AE1/AE3 and 8/18 were stained intracitoplasmatic positive, accentuated (+++) and moderate (++), respectively in tubular epithelia in more than 50% of the cells. There was immunostaining for vimentin in the cytoplasm of mesenchymal neoplastic cells, cartilaginous and tubular epithelial cells with moderate intensity (++) in less than 50% of the cells. Discussion: Carcinosarcoma is a rare neoplasm in cats and have being reported in the mammary gland and in the lungs. The neoplasm is characterized by mesenchymal and epithelial cells. In many cases the histological exam is insufficient to determinate the diagnosis, being necessary to do the immunohistochemistry (IHC) exam to verify vimentin and cytokeratin coexpression in neoplasic cells. Histologically, was observed epithelial and mesenchymal characteristics, confi rmed by IHC. In another paper, carcinosarcoma demonstrated similar arranged, as heterogenic cellular composition and infiltrative proliferation of mesenchymal cells. In this case report was also observed cartilaginous and osseous differentiation. Some carcinosarcoma can present differentiated mesenchymal tissue and a mixture of all carcinomatosos and sarcomatosos elements in the same neoplasic mass. Histogenesis of carcinosarcoma remains uncertain thus far, although two theories suggest its origin: a multiclonal and other monoclonal. The first one suggests that mesenchymal and epithelial cells are originated from two or more different base cells. The second theory suggests that the tumors behavior is the result of a totipotent ability of neoplasic cells to differ and be histologically recognizable as epithelial and mesenchymal structures. In this study, tubular epithelial cells were positive stained for cytokeratins (AE1/AE3 and 8/18) and to vimentin, which suggest a monoclonal origin, because it was observed epithelial and mesenchymal differentiation. According to localization, morphology features and immunohistochemistry evaluation, the present study reports a rare case of carcinosarcoma in a cat.