Evaluation of the thermoregulatory response of goats naturally exposed to the tropical climate using longitudinal data / Avaliação da resposta termorregulatória de cabras expostas naturalmente ao clima tropical a partir do uso de dados longitudinais

Rectal temperature (RT), heart rate (HR), and respiratory rate (RR), determined as repeated measurements over time in female goats, were used to identify covariance matrices that best fit the data for residual modeling on these three traits. Then, based on this result, the goats' responses to heat were evaluated. Five matrices were found with convergence for the three traits. The Heterogeneous Compound Symmetry matrix showed a good fit for modeling the residual associated with RT, whereas the Heterogeneous Autoregressive matrix had a better fit for RR and HR, according to the Akaike Information Criteria (AIC), corrected AIC (AICc), and Schwarz Bayesian Information Criterion (BIC) used. After adjusting the residual data for these three traits, a mixed-model analysis was used to evaluate collection period (3), physiological stage (3), and animal age (3) as fixed effects. Residual modeling interfered differently with the p-value associated with the fixed effects studied. Collection period and interactions did not influence the variation in RT (P>0.761), which was within the standard range for goats in the tropics, while the physiological stage of the goats affected it (P<0.05). Rectal temperature, HR, and RR tend to show covariance structures that can be modeled using specific residual covariance matrices, that is, the heterogeneous compound symmetry matrix best suits RT data, whereas the heterogeneous autoregressive matrix is better suited for HR and RR, which are usually correlated. The goats of the evaluated breed maintain RT within the range of variation displayed by breeds adapted to a hot environment, regardless of their physiological condition. Variations occur in RR and HR, without, however, exceeding the normal range for goats. Pregnancy causes goats to raise their RR in the rainy season of the year in the region in order to maintain RT within the normal range for the species.(AU)

Utilizou-se a Temperatura retal (TR), Frequências cardíaca (FC) e respiratória (FR) aferidas como medidas repetidas no tempo em fêmeas caprinas, objetivando-se identificar matrizes de estruturas de covariância que melhor se ajustou aos dados para modelagem do resíduo nessas três características e, em seguida, avaliou-se a respostas de cabras ao calor, com base nesse resultado. Constatou-se cinco matrizes com convergência nas três características. A Simétrica composta heterogênea ajustou-se bem para modelagem do resíduo associado a TR, enquanto a Autorregressiva heterogênea ajustou-se melhor para a FR e FC, de acordo com os critérios de informação de Akaike (AIC), Akaike corrigido (AICc) e o Bayesiano de Schwarz (BIC) utilizados. Com o resíduos de dados dessas três características ajustados, utilizou-se uma análise com modelos mistos para avaliar a Época de coleta (3), Estado fisiológico (3) e Idade do animal (3) foram como efeitos fixos. Constatou-se que a modelagem do resíduo interferiu de modo diferenciado no p valor associado aos efeitos fixos estudados. A época da coleta e interações não influenciaram a variação da TR (P>0,761), que oscilou dentro da faixa padrão para caprinos nos trópicos, mas o Estágio fisiológico da cabra sim (P<0,05). A Temperatura retal e as Frequências cardíaca e respiratória tendem a apresentar estruturas de covariâncias modeláveis com utilização de matrizes de covariâncias residuais especificas, ou seja, a matriz Simétrica composta heterogênea mais adequada para dados da Temperatura retal, enquanto a Autorregressiva heterogênea para as Frequências cardíaca e respiratória, geralmente correlacionas. As cabras da raça avaliadas mantêm a temperatura retal dentro da amplitude de variação apresentada por raças adaptadas a ambiente quente. Isso ocorre independente da condição fisiológica que se encontra, mas com ocorrência de variação na frequência respiratória e cardíaca, não excedendo, no entanto, a faixa normal para caprinos. A gestação condiciona a cabra a elevar a FR na época chuvosa do ano na região para manter a TR na faixa de amplitude normal para caprinos.(AU)

Dietary polymer-coated urea enhances the goats lactational performance, excretion of microbial purine derivatives and blood metabolites in the semi-arid zone of Iran

This study aimed to determine the effect of using different sources of nitrogen to supply part of degradable intake protein needs in lactating goat performance and its effect on excretion of microbial purine derivatives and blood metabolites. Thirty-two lactating Saanen goats (body weight 38.85 ± 2.14 kg and 1979 ± 0.25 gday-1milk yield) were used in a one-way ANOVA completely randomized design. Goats were assigned to the following treatments for a 10-wk experimental period: 1) Control (canola meal as a nitrogen source); 2) Urea (0.5% urea); 3) Optigen (0.55% Optigen-Alltech.Inc., Lexington, KY) and 4) Polymer-Coated Urea (PCU-international patent number: A01K5/00, 0.7% PCU) based on dry mater intake. Non-protein nitrogen groups had a comparative effect (p > 0.05) between control and other treatments on milk composition, microbial protein synthesis and they affected on blood factors including urea, cholesterol, and ALT. Dry matter intake decreased (p > 0.05) in PCU, Optigen, Urea than Control goats. Synthesis of microbial protein in PCUgoats was 22.5 g day-1and it was greater (p > 0.05) than other treatments. Plasma cholesterol was increased in PCUand Optigen, whereas urea concentration was increased in Urea and Control goats. Milk production was higher in PCUthan Urea and Control. Feed conversion ratio was improved (p > 0.05) in PCU and Optigengoats versusother treatments.This study demonstrated that polymer-coated ureacan be utilized as a nitrogen source and improve goats milk performance.(AU)

Impact of high-fat diet consumption during prolonged period of pregnancy on placenta structures and umbilical vascular growth in goats

This study aimed to verify the impact of high-fat diet consumption for a prolonged period on oxidative stress, fetal growth, umbilical vascular system, and placental structures in pregnant goats. Twenty-two pregnant goats were grouped into the control diet (n= 11) and fat diet (n = 11). Flaxseed meal was added to the fat diet, replacing the corn grain of concentrate, from gestational day 100 to delivery date. Diets were isonitrogenous and isoenergetic, differing in fat content (2.8% vs. 6.3% dry matter). The fat group showed higher feed intake and total plasma lipid levels than the control group (P < 0.001). No difference was found in placentome, and umbilical vascular development. Fat diet-fed goats exhibited a lower systolic peak in the umbilical artery. At delivery, placental traits were similar with the exception of the cotyledon width (P = 0.0075), which was smaller in the fat group and cotyledon surface (P = 0.0047) for multiple pregnancy of fat diet. Cotyledonary epithelium showed more intense staining of lipid droplets and a greater area for lipofuscin staining in the fat group compared to control group (P < 0.001). The mean live weight of the kids was lower in the fat group in the first week after delivery than in control group. Thus, in goats, the continuous administration of a high-fat diet during pregnancy does not appear to modify the fetal-maternal vascular structures but has an impact on a part of the placental structure; therefore, its use must be carefully evaluated.(AU)

Maternal and fetal ultrasonography, vulvar temperature and vaginal mucous impedance for the prediction of parturition in Saanen does

The aim of this study was to evaluate and correlate modifications of vaginal mucous impedance, vulvar temperature and ultrasonographic measurements (echobiometric parameters) to parturition in pregnant Saanen does. 30 does were selected for the study and submitted to an estrus synchronization protocol and natural mating. The females were evaluated daily from Day 143 of pregnancy to parturition. For the sonographic evaluations, the following structures were measured: biparietal diameter, thoracic diameter, abdominal diameter, ocular orbit, kidney length, kidney height, cardiac area, placentome length, cervical measurement and fetal heart rate; by means of two different approaches: transrectal and transabdominal, using a 7.5 MHz linear transducer. The vaginal mucous impedance was assessed using an electric estrous detector and vulvar temperature was measured using a non-contact infrared thermometer. Statistical analysis was performed using the R-project software and the significance level was set at 5% for all tests. 25 Saanen does became pregnant, resulting in 80.33% pregnancy rate. Fetal heart rate was negatively correlated to the hours to parturition (p<0,001; r-Pearson= -0,451), as well as vaginal temperature (p= 0,001; r-Pearson= -0,275), while cervical thickness was positively correlated to hours to parturition (p<0,001; r-Pearson= 0,490). The echobiometric parameters (biparietal diameter, thoracic diameter, abdominal diameter, ocular orbit, kidney length and height, cardiac area, placentome length), as well as vaginal mucous impedance did not vary throughout the timepoints of evaluation and did not correlate to the moment of parturition. It was concluded that the parameters of fetal heartbeat, vaginal temperature and cervical effacement in the last week of pregnancy provide valuable information regarding the proximity of parturition.(AU)

Uso da ultrassonografia Doppler colorido na reprodução de pequenos ruminantes / Use of color Doppler ultrasonography in the reproduction of small ruminants

A ultrassonografia (US) Doppler colorido fornece uma ferramenta não invasiva valiosa para detectar e monitorar mudanças dinâmicas na rede vascular e fluxo sanguíneo em vários órgãos e tecidos reprodutivos. Em apoio as biotécnicas da reprodução animal assistida, a US Doppler colorido tem mostrada alta eficiência no monitoramento funcional das estruturas ovarianas. A previsão de respostas ovarianas e produções embrionária em ovinos pela identificação de sinais Doppler na parede folicular já se mostrou eficiente. A aplicação da US Doppler colorido para a identificação da funcionalidade do tecido luteal é ainda maior, desde acompanhamentos fisiológicos e diagnósticos de disfunções luteais até ampla aplicação em conjunto as diferentes biotécnicas reprodutivas. Destaca-se em ovelhas e cabras, a aplicação comercial da US Doppler colorido para o diagnóstico de gestação precoce, de disfunções luteais, de determinação de respostas ovarianas em fêmeas doadoras e receptoras de embriões, para identificar efeitos luteotrófico de estratégias hormonais, e ainda para amparar as estratégias de ressincronização de estro.(AU)

Color Doppler ultrasonography (US) provides a valuable non-invasive tool for detecting and monitoring dynamic changes in the vascular network and blood flow in various reproductive organs and tissues. In support of assisted animal reproduction biotechniques, color Doppler US has shown high efficiency in the functional monitoring of ovarian structures. The prediction of ovarian responses and embryonic production in sheep by identifying Doppler signals in the follicular wall has already proved to be efficient. The application of color Doppler US for identifying the functionality of the luteal tissue is even greater, from physiological monitoring and diagnosis of luteal dysfunctions to wide application together with different reproductive biotechniques. It stands out in sheep and goats, the commercial application of color Doppler US for the diagnosis of early pregnancy, luteal dysfunctions, determination of ovarian responses in embryo donor and recipient females, to identify luteotrophic effects of hormonal strategies, and even to support estrus resynchronization strategies.(AU)

Exploring the rumen microbial community in Guizhou White goats at different ages

This study evaluated the changes in rumen microbiome during the process of age development of farming Guizhou White goats from Southwest China. We conducted high-throughput 16S rRNA gene sequencing to investigate the diversity, structure, and composition of goat rumen microbiota (RM) of 21 goats of different age groups (1, 6, and 12 months). We found that volatile fatty acids (i.e., acetate, propionate, and butyrate) fermented by microbes were found to increase significantly in the sixand one-month-old goats. Results of the genera abundance analysis showed that abundance of eight and seven taxa decreased in six- and one-month-old goats, respectively, compared with that in 12-month-old goats. Additionally, differences in six taxa in six-month-old goats and in one taxon in one-month-old goats were found. In addition, specific gut microbiome was found, which was significantly correlated with rumen fermentation parameters in Guizhou White goats. These results revealed the signature microbiota in RM during various developmental stages in goats raised in Southwest China and can also provide a guiding tool for evaluating rumen health of ruminants worldwide.(AU)

Physiological adaptability of pregnant doe Kacang goats in a dry-land-area of Indonesia

The objective of the present study was to evaluate the physiological adaptability of pregnant doe Kacang goats in a dryland area of Indonesia. Thirty pregnant doe Kacang goats aged 2-3 years were observed. The rearing system was semiintensive without giving concentrated feeds. Local grasses and legumes such as Leucaena leucocephala leaves, Gliricidia sepium leaves, Sesbania grandiflora leaves, and drinking water were offered ad libitum. Experimental data such as respiration rate, rectal temperature, adaptability coefficient, heart rate, and heat tolerance coefficient, were collected during 2-3 months of gestation. The mean and standard deviation were calculated using a descriptive analysis method. The average humidity in the morning and afternoon and the ambient temperature in the middle of the day were outside the normal range. The THI value indicates that the experimental animals are under medium heat stress. The average values of HTC, AC, RT, HR, and RR were still typical for goats. In conclusion, although the average ambient temperature at midday and humidity in the morning and afternoon were outside of the normal range, they did not cause any effects on feed and water intake, health, and fetus growth and development of pregnant doe Kacang goats. This happened because the Kacang goat is a local breed that can adapt well to extreme environments. Therefore, pregnant doe Kacang goats in Malaka District can be allowed to graze in the paddock throughout the day and housed at night.

Cumulative effect of different acute stressors on physiological and hormonal responses and milk yield in lactating Saanen goats / Efeito cumulativo de diferentes estressores agudos sobre as respostas fisiológicas, hormonais e produção de leite de cabras Saanen em lactação

This study addresses the hypothesis that acute stress can cumulatively cause a decrease in milk yield in Saanen goats. In fact, dairy animals are subject to several environmental and management challenges that may cause acute stress during the same lactation. However, the cumulative effect of acute stress on milk yield remains unclear. Thus, the objective of this study was to evaluate the effects of different acute stressors on milk yield and milk quality in goats. Thirty Saanen goats were either maintained on their usual routine and comfort conditions (control group) or subjected to different environmental stressors (heat stress, adrenocorticotropic hormone [ACTH] administration, hoof care, and rain). These stressful challenges were performed sequentially, one challenge per day, on four consecutive days to evaluate the influence of the challenge on milk yield and milk quality. The acute stress imposed on goats caused significant changes in respiratory rate, rectal temperature, cortisol, insulin, triiodothyronine, insulin-like growth factor 1, and glucose concentrations when compared to the control group. Although these acute-stress-triggered physiological responses are fundamental to restoring homeostasis, the cumulative effects of different imposed challenges caused a change in hormone release, an increase in somatic cell count (SCC), and a decrease in milk yield. In this context, the results of this study indicate that farmers should avoid concurrently subjecting goats to management and environmental challenges that can induce acute stress because these stressors have a negative and cumulative impact on SCC and milk yield.(AU)

Este estudo aborda a hipótese de que o estresse agudo pode causar cumulativamente uma diminuição na produção de leite de cabras Saanen. De fato, os animais leiteiros estão sujeitos a vários desafios ambientais e de manejo que podem causar estresse agudo durante a mesma lactação. No entanto, o efeito cumulativo do estresse agudo na produção de leite ainda não está claro. Assim, o objetivo deste estudo foi avaliar os efeitos de diferentes estressores agudos na produção e qualidade de leite de cabras Saanen. Trinta cabras Saanen foram mantidas em sua rotina habitual e condições de conforto (grupo controle) ou submetidas a diferentes estressores ambientais (estresse por calor, administração de hormônio adrenocorticotrófico [ACTH], cuidados com os cascos e chuva). Esses desafios estressantes foram realizados sequencialmente, um desafio por dia, em quatro dias consecutivos para avaliar a influência do desafio na produção e qualidade do leite. O estresse agudo imposto as cabras causou alterações significativas na frequência respiratória, temperatura retal, cortisol, insulina, triiodotironina, fator de crescimento semelhante à insulina 1 e concentrações de glicose quando comparado ao grupo controle. Embora essas respostas fisiológicas desencadeadas pelo estresse agudo sejam fundamentais para restaurar a homeostase, os efeitos cumulativos de diferentes desafios impostos causaram uma mudança na liberação de hormônios, um aumento na contagem de células somáticas (CCS) e uma diminuição na produção de leite. Neste contexto, os resultados deste estudo indicam que os produtores devem evitar submeter as cabras concomitantemente a desafios de manejo e ambientais que possam induzir estresse agudo, pois esses estressores têm impacto negativo e cumulativo na CCS e na produção de leite.(AU)

Production, intake, and feeding behavior of dairy goats fed alfalfa via grazing and cassava

This study examined the replacement of maize and soybean meal with cassava chips and alfalfa grazing, respectively. Twelve lactating Anglo-Nubian goats were kept on a Panicum maximum cv. Tobiatã pasture. The experiment was laid out in a Latin square design in which the following diets were tested: ground maize + soybean meal, cassava chips + soybean meal, ground maize + alfalfa grazing, and cassava chips + alfalfa grazing. The evaluated variables were feed intake, daily weight gain, milk yield and composition, and feeding behavior of the goats as well as production costs. Cassava chips and grazed alfalfa influenced the intakes of dry matter, crude protein, neutral detergent fiber, and total digestible nutrients. However, milk yield, body weight, and body score did not change. There was no diet effect on the proportions of protein, solids-not-fat, somatic cell count, or urea nitrogen in the milk. Treatments influenced the levels of fat, lactose, and total solids in milk, with the highest fat levels achieved with diets containing alfalfa. Grazing, rumination, and idle times and time spent interacting with other goats were not influenced by diets. The evaluated feedstuffs improved feed efficiency and reduced production costs. Therefore, cassava chips and alfalfa can replace certain ingredients without impairing the production performance of goats, but rather improving the profit of the producer.(AU)

Outbreak of abomasal bloat in goat kids due to Clostridium ventriculi and Clostridium perfringens type A

We described an outbreak of abomasal bloat in goat kids. Increased mortality with a history of abdominal bloating, dullness, and death was reported. Postmortem exanimation revealed dehydration, pale mucosa, ascites, abomasal tympanism and intestinal meteorism and congestion, and emphysematous abomasitis. Cytological evaluation from abomasum revealed gram-positive bacteria with a cuboid shape suggestive of Clostridium ventriculi, gram-positive bacilli suggestive of Clostridium perfringens, and ovoid basophilic yeasts. C. ventriculi and C. perfringens type A were confirmed by anaerobic culture and molecular tests. Histopathological findings revealed emphysematous abomasitis, necrosis of the gastric and intestinal walls, gastroenteritis, and intestinal thromboembolism. The possible associated factor was erroneous use of the milk replacer associated with inadequate kid management. Finally, the prophylactic measures suggested such as hygiene care, proper use of milk replacer, clostridial vaccination plan, and a good colostrum management were able to control the outbreak.(AU)

Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

Kacang goats are small ruminants produced by low-income households in smallholder and farm to reduce poverty and prevent undernutrition. Studies to find a cryopreservation protocol for Kacang goat semen are expected to multiplication of genetically superior animals selected by the paternal lineage. This study evaluated the effect of thawing temperature and supplementation of the green tea extract nanoparticle in skim milk-egg yolk (SM-EY) extender on post-thaw sperm quality of Kacang goat semen. Six ejaculates of Kacang goat were diluted in SM-EY supplemented or not (control group) with 0.001 mg/mL NPs GTE. The diluted semen was packaged with 0.25 mL straws (insemination dose: 60x106 sptz/mL) and cryopreserved. Then, six samples of the control group and NPs GTE groups were thawed at 37°C or 39°C sterile water for 30 s and submitted to sperm quality evaluations. The sperm viability, motility, and intact of the plasma membrane (IPM) were higher (p<0.05) in NPs GTE group than control group. In contrast, the NPs GTE group presented lower (p<0.05) malondialdehyde levels and sperm DNA fragmentation (SDF) compared with the control group. The catalase levels were not significantly different (p > 0.05) between the control and NPs GTE groups. Thawing at 39°C resulted in higher (p<0.05) sperm viability, motility, and IPM than thawing at 37°C. However, thawing at 39°C group presented lower (p<0.05) malondialdehyde levels compared with thawing at 37°C. SDF and catalase levels were similar (p>0.05) between thawing at 37°C and thawing at 37°C. In conclusion, supplementation of 0.001 mg/mL of NPs GTE in SM-EY extender and thawing temperature of 39°C resulted in a better quality of frozen-thawed Kacang goat semen.(AU)

Replacement of corn with pre-dried cassava root silage in the diet for dairy goats

The objective of this study was to evaluate the effect of replacement of corn with pre-dried cassava root silage (CRS) on intake, ingestive behavior, production and composition of goat milk. Five lactating Saanen goats were used, with 42.5 ± 4.2 kg body weight and production of 2 kg milk day-1, distributed in a 5 x 5 Latin square with five treatments: 0, 28.6, 58.0, 81.6 and 100% replacement of corn with CRS in the diet dry matter. Goats were monitored for five periods of 15 days each. The intake of dry matter, crude protein and neutral detergent fiber decreased linearly (p <0.05) with the replacement of corn with CRS. Feeding (348.5 ± 62.1 min.) and rumination (468.8 ± 83.8 min.) times were not influenced (p >0.05) by the replacement of corn with CRS, but the ingestion and rumination efficiencies had a linear increase (p > 0.05). Milk production (2.17 ± 0.45 kg day-1), fat (3.49 ± 0.42%), protein (3.11 ± 0.13%) and lactose (4.47 ± 0,11%) of goat milk were not influenced (p >0.05) by the replacement of corn with CRS. Therefore, it is recommended the total replacement of corn with pre-dried cassava root silage in the diet for dairy goats with an average production of 2 kg day-1.(AU)

Influence of eCG and reproductive management in the resynchronization of ovulation in dairy goats

Resynchronization protocols have been proposed as a way of shortening females' unproductive time in the flock, with good results in cattle and sheep. In goats, initial studies have shown that a second progestogen device inserted before luteolysis and pregnancy diagnosis does not interfere with the corpus luteum lifespan or functionality. This study aimed to evaluate the follicular growth, ovulation pattern and pregnancy rate after insertion of a second and new progestogen device for resynchronizing, with or without equine Chorionic Gonadotrophin (eCG), submitted to natural mating (NM) or artificial insemination (AI) to propose a viable resynchronization protocol for dairy goats. A total of 38 multiparous Saanen goats underwent a short-term progesterone protocol [six days exposed to medroxyprogesterone acetate (MAP) intravaginal sponges + 200 IU eCG and 0.12 mg of cloprostenol sodium on the 5th day + 0.025 mg of lecirelin 34 hours after sponge withdrawal] and, on day 16th after the ovulation, received a new MAP device which was retained until day 21. At this moment females were split into four groups: GeCG+NM ­ 100 IU eCG with NM; GSal+NM ­ saline solution with NM; GeCG+AI ­ 100 IU eCG with AI; and GSal+AI ­ saline solution with AI. Ultrasound scans were performed every 12 h from sponge withdrawal (day 21) until 108 h after sponge withdrawal (day 25) for follicular dynamics evaluation, at 240 h (day 31) for assessing the presence of active corpus luteum, and on day 60 for pregnancy diagnosis. No differences were found regarding ovulation time, synchronization and follicle size. However, GeCG+NM presented a greater estrus manifestation rate (100%) and pregnancy rate (62.5%) when compared to GSal+AI. In conclusion, resynchronization protocols in dairy goats may present satisfactory results.(AU)

Distribution of extracellular matrix related proteins in normal and cryptorchid ziwuling black goat testes

The Ziwuling black goat is an indigenously in China, their offspring are frequently affected by congenital cryptorchidism. The extracellular matrix (ECM) contains cytokines and growth factors that regulate the development of the testis, and component changes often result in pathological changes. Cryptorchidism is closely related to structural changes in ECM. In this study, the histochemical staining, immunohistochemical, immunofluorescence and Western blot combined with semi-quantitative analysis was used to describe the distribution of the important ECM components Collagen type IV (Col IV), laminin (LN)and heparan sulfate proteoglycans (HSPG) in the normal and cryptorchid testes of Ziwuling black goats. Results showed that: The histochemical staining showed that the dysplasia of seminiferous tubules and decreased number of Sertoli cells in cryptorchidism, as well as sparse collagen fiber. Meanwhile, the distribution of reticular fibers is relatively rich. Furthermore, the PAS and AB staining in the interstitial vessels and lamina propria of seminiferous tubules is weak. The immunohistochemical and immunofluorescence revealed that Col IV, LN was strongly expressed in Leydig, Sertoli cells of normal testes and moderately positive in the spermatogonia and spermatids, but HSPG was not expressed in the spermatogonia. However, cryptorchidism, the expression of Col IV, LN and HPSG in Leydig, Sertoli cells significantly decreased, as well as the expression of Col IV and LN in capillary endothelial cells, but HSPG was moderately expressed in spermatogonia. Based on these data, the underdevelopment of spermatogenic epithelium, decreased synthesis function of collagen fibers and Leydig cells develop usually in the cryptorchidism were shown to be closely related to the abnormal metabolism of Col IV and LN. The positive expressed of HSPG in the spermatogonia of cryptorchid testes is related to the compensatory development of spermatogonia.(AU)

Dry matter intake and ruminal fermentation parameters in goats fed sugarcane in replacement of corn silage / Parâmetros de ingestão de matéria seca e fermentação ruminal em cabras alimentadas com cana-de-açúcar em substituição à silagem de milho

The trial was conducted with the objective to evaluate fresh sugarcane as a replacement for corn silage on dry matter intake, ruminal parameters (pH, short chain fatty acids, and ammonia nitrogen concentration), and degradation kinetics of forages and concentrate in maintenance adult goats. Four nonlactating ruminally cannulated goats were used in a 4 × 4 Latin square design with the replacement of 0, 33, 67, and 100% of corn silage by sugarcane as the independent variable. The substitution of sugarcane for corn silage did not affect dry matter intake, daily weight gain, or ruminal pH. A quadratic effect was observed for ammonia nitrogen concentration with a maximum concentration at 74.43% of sugarcane inclusion, as well as for short chain fatty acids, with maximum concentrations of acetate and butyrate at 0.59 and 32.70% of sugarcane inclusion, respectively. The minimum concentration of propionate was verified on 10.36% of sugarcane inclusion. However, the acetate:propionate ratio linearly decreased with increased sugarcane content. No effect was observed of different rumen environments on forage dry matter and neutral detergent fiber degradability (effective and potential degradability, soluble and potential degradable fractions). Dry matter degradability was 66.79% for sugarcane and 72.62% for corn silage after 96 h of incubation. Neutral detergent fiber was 37.10 and 51.82% for sugarcane and corn silage, respectively, after 96 h of incubation. In conclusion, sugarcane can be used as a replacement for corn silage in maintenance adult goats feed without altering dry matter intake and forage degradability while increasing propionate production.(AU)

O experimento foi conduzido para avaliar a cana-de-açúcar in natura em substituição à silagem de milho no consumo de matéria seca, parâmetros ruminais e cinética de degradação de forragens e concentrado em cabras adultas em mantença. Quatro cabras não lactantes canuladas no rúmen foram utilizadas em um delineamento em quadrado latino 4 × 4 com a substituição de 0, 33, 67 e 100% da silagem de milho por cana-de-açúcar. A substituição da cana-de-açúcar pela silagem de milho não afetou o consumo de matéria seca, o ganho de peso diário e o pH ruminal. Foi observado efeito quadrático para a concentração de nitrogênio amoniacal com concentração máxima de 74,43% de inclusão da cana, e também para ácidos graxos de cadeia curta, com concentrações máximas de acetato e butirato de 0,59 e 32,70% de inclusão da cana, respectivamente. A concentração mínima de propionato foi verificada em 10,36% de inclusão de cana-de-açúcar. No entanto, a relação acetato: propionato diminuiu linearmente com o aumento do teor de cana-de-açúcar. Não foi observado efeito de diferentes ambientes ruminais sobre a degradabilidade da matéria seca da forragem e da fibra em detergente neutro. A degradabilidade da matéria seca foi de 66,79% para a cana-de-açúcar e 72,62% para a silagem de milho após 96 h de incubação. A fibra em detergente neutro foi 37,10 e 51,82% para cana-de-açúcar e silagem de milho, respectivamente, após 96 h de incubação. Em conclusão, a cana-de-açúcar pode ser usada como substituto da silagem de milho na alimentação de cabras adultas de manutenção, sem alterar o consumo de matéria seca e a degradabilidade da forragem, aumentando a produção de propionato.(AU)

Spontaneous poisoning by Ateleia glazioviana (leg. Papilionoideae) in sheep and goats in the West region of Santa Catarina / Intoxicação espontânea por Ateleia glazioviana (Leg. papilionoideae) em ovinos e caprinos no Oeste de Santa Catarina

This study reported the epidemiological data and the clinical and pathological conditions of spontaneous poisoning by Ateleia glazioviana in sheep and goats in the West region of Santa Catarina. The small ruminants were located in a place where there was a large amount of young plants of A. glazioviana and showing signs of consumption. The stock of sheep was composed of 250 animals, of which 45 died. In the goat herd, there were 28 animals, and of these, 27 died, and one was sacrificed. The main clinical signs were weight loss, fatigue, slow-walk, and eventually submandibular and facial edema. Some animals were found dead, and others died suddenly after being moved. Abortions and the birth of weak lambs were also observed. In total, five sheep and one goat were necropsied. The main macroscopic lesions were associated to eccentric cardiac hypertrophy and moderate, multifocal white areas in the epicardium and myocardium. Two sheep showed marked edema in the subcutaneous tissue of the head and neck and it could be noted hydrothorax and hydroperitoneum and liver with a nutmeg-like appearance. Histological changes were relevant in the myocardium. They consisted of fibrosis and myofiber necrosis associated with macrophage infiltrate, multifocal, low and marked swelling of cardiomyocytes with loss of fibrillarystriae, and increased nuclear volume. In two sheep, there was still congenital hepatic, as well as marked centrilobular and diffuse areas.(AU)

Descrevem-se dados epidemiológicos, sinais clínicos e lesões da intoxicação espontânea por Ateleia glazioviana em ovinos e caprinos no Oeste de Santa Catarina. Os ovinos e caprinos se encontravam em um local que havia grande quantidade de A. glazioviana jovem e com sinais de consumo. O plantel de ovinos era composto por 250 animais, dos quais 45 morreram. No rebanho caprino haviam 28 animais e destes, 27 morreram e um foi sacrificado. Os principais sinais clínicos se caracterizaram por perda de peso, cansaço, caminhar lento e eventualmente edema submandibular e facial. Alguns foram encontrados mortos e outros morreram subitamente após serem movimentados. Abortos e nascimento de cordeiros fracos também foi observado. No total, cinco ovinos e um caprino foram necropsiados. As principais lesões macroscópicas foram hipertrofia excêntrica do coração e áreas brancas multifocais, moderadas, no epicárdio e miocárdio. Dois ovinos apresentaram edema acentuado no tecido subcutâneo da cabeça e pescoço, bem como hidrotórax, hidroperitôneo e fígado com aspecto de noz-moscada. As alterações histológicas foram relevantes no miocárdio e consistiram de áreas de fibrose e necrose de miofibras associada à infiltrado de macrófagos, multifocal, leve, além de tumefação acentuada de cardiomiócitos com perda das estrias fibrilares e aumento do volume nuclear. Em dois ovinos, havia ainda, congestão hepática, centrolobular, difusa.(AU)

Spontaneous poisoning by Ateleia glazioviana (leg. Papilionoideae) in sheep and goats in the West region of Santa Catarina / Intoxicação espontânea por Ateleia glazioviana (Leg. papilionoideae) em ovinos e caprinos no Oeste de Santa Catarina

This study reported the epidemiological data and the clinical and pathological conditions of spontaneous poisoning by Ateleia glazioviana in sheep and goats in the West region of Santa Catarina. The small ruminants were located in a place where there was a large amount of young plants of A. glazioviana and showing signs of consumption. The stock of sheep was composed of 250 animals, of which 45 died. In the goat herd, there were 28 animals, and of these, 27 died, and one was sacrificed. The main clinical signs were weight loss, fatigue, slow-walk, and eventually submandibular and facial edema. Some animals were found dead, and others died suddenly after being moved. Abortions and the birth of weak lambs were also observed. In total, five sheep and one goat were necropsied. The main macroscopic lesions were associated to eccentric cardiac hypertrophy and moderate, multifocal white areas in the epicardium and myocardium. Two sheep showed marked edema in the subcutaneous tissue of the head and neck and it could be noted hydrothorax and hydroperitoneum and liver with a nutmeg-like appearance. Histological changes were relevant in the myocardium. They consisted of fibrosis and myofiber necrosis associated with macrophage infiltrate, multifocal, low and marked swelling of cardiomyocytes with loss of fibrillarystriae, and increased nuclear volume. In two sheep, there was still congenital hepatic, as well as marked centrilobular and diffuse areas.(AU)

Descrevem-se dados epidemiológicos, sinais clínicos e lesões da intoxicação espontânea por Ateleia glazioviana em ovinos e caprinos no Oeste de Santa Catarina. Os ovinos e caprinos se encontravam em um local que havia grande quantidade de A. glazioviana jovem e com sinais de consumo. O plantel de ovinos era composto por 250 animais, dos quais 45 morreram. No rebanho caprino haviam 28 animais e destes, 27 morreram e um foi sacrificado. Os principais sinais clínicos se caracterizaram por perda de peso, cansaço, caminhar lento e eventualmente edema submandibular e facial. Alguns foram encontrados mortos e outros morreram subitamente após serem movimentados. Abortos e nascimento de cordeiros fracos também foi observado. No total, cinco ovinos e um caprino foram necropsiados. As principais lesões macroscópicas foram hipertrofia excêntrica do coração e áreas brancas multifocais, moderadas, no epicárdio e miocárdio. Dois ovinos apresentaram edema acentuado no tecido subcutâneo da cabeça e pescoço, bem como hidrotórax, hidroperitôneo e fígado com aspecto de noz-moscada. As alterações histológicas foram relevantes no miocárdio e consistiram de áreas de fibrose e necrose de miofibras associada à infiltrado de macrófagos, multifocal, leve, além de tumefação acentuada de cardiomiócitos com perda das estrias fibrilares e aumento do volume nuclear. Em dois ovinos, havia ainda, congestão hepática, centrolobular, difusa.(AU)

Dry matter intake and ruminal fermentation parameters in goats fed sugarcane in replacement of corn silage / Parâmetros de ingestão de matéria seca e fermentação ruminal em cabras alimentadas com cana-de-açúcar em substituição à silagem de milho

The trial was conducted with the objective to evaluate fresh sugarcane as a replacement for corn silage on dry matter intake, ruminal parameters (pH, short chain fatty acids, and ammonia nitrogen concentration), and degradation kinetics of forages and concentrate in maintenance adult goats. Four nonlactating ruminally cannulated goats were used in a 4 × 4 Latin square design with the replacement of 0, 33, 67, and 100% of corn silage by sugarcane as the independent variable. The substitution of sugarcane for corn silage did not affect dry matter intake, daily weight gain, or ruminal pH. A quadratic effect was observed for ammonia nitrogen concentration with a maximum concentration at 74.43% of sugarcane inclusion, as well as for short chain fatty acids, with maximum concentrations of acetate and butyrate at 0.59 and 32.70% of sugarcane inclusion, respectively. The minimum concentration of propionate was verified on 10.36% of sugarcane inclusion. However, the acetate:propionate ratio linearly decreased with increased sugarcane content. No effect was observed of different rumen environments on forage dry matter and neutral detergent fiber degradability (effective and potential degradability, soluble and potential degradable fractions). Dry matter degradability was 66.79% for sugarcane and 72.62% for corn silage after 96 h of incubation. Neutral detergent fiber was 37.10 and 51.82% for sugarcane and corn silage, respectively, after 96 h of incubation. In conclusion, sugarcane can be used as a replacement for corn silage in maintenance adult goats feed without altering dry matter intake and forage degradability while increasing propionate production.

O experimento foi conduzido para avaliar a cana-de-açúcar in natura em substituição à silagem de milho no consumo de matéria seca, parâmetros ruminais e cinética de degradação de forragens e concentrado em cabras adultas em mantença. Quatro cabras não lactantes canuladas no rúmen foram utilizadas em um delineamento em quadrado latino 4 × 4 com a substituição de 0, 33, 67 e 100% da silagem de milho por cana-de-açúcar. A substituição da cana-de-açúcar pela silagem de milho não afetou o consumo de matéria seca, o ganho de peso diário e o pH ruminal. Foi observado efeito quadrático para a concentração de nitrogênio amoniacal com concentração máxima de 74,43% de inclusão da cana, e também para ácidos graxos de cadeia curta, com concentrações máximas de acetato e butirato de 0,59 e 32,70% de inclusão da cana, respectivamente. A concentração mínima de propionato foi verificada em 10,36% de inclusão de cana-de-açúcar. No entanto, a relação acetato: propionato diminuiu linearmente com o aumento do teor de cana-de-açúcar. Não foi observado efeito de diferentes ambientes ruminais sobre a degradabilidade da matéria seca da forragem e da fibra em detergente neutro. A degradabilidade da matéria seca foi de 66,79% para a cana-de-açúcar e 72,62% para a silagem de milho após 96 h de incubação. A fibra em detergente neutro foi 37,10 e 51,82% para cana-de-açúcar e silagem de milho, respectivamente, após 96 h de incubação. Em conclusão, a cana-de-açúcar pode ser usada como substituto da silagem de milho na alimentação de cabras adultas de manutenção, sem alterar o consumo de matéria seca e a degradabilidade da forragem, aumentando a produção de propionato.

Inseminação artificial em tempo fixo em pequenos ruminantes: desafio de um programa comercial / Artificial insemination at a fixed time in small ruminants: challenge of a commercial program

A Inseminação Artificial é uma das biotécnicas da reprodução animal mais utilizadas em pequenos ruminantes, contribuindo na incessante busca pelo melhoramento animal nesta espécie e apresentando-se viável ao pequeno, médio e grande produtor. Pelo método convencional, com observação de cio, ou com a utilização de protocolos de sincronização do estro e indução de ovulação, com uso da Inseminação Artificial em Tempo Fixo (IATF), diversos são os desafios encontrados quando da execução de um programa comercial. Entendendo a não padronização de manejo nas diversas unidades produtivas, bem como, a pouca ou nenhuma atenção dada, por grande parte destas, a questões de bem-estar animal, envolvendo conforto térmico, atendimento as necessidades nutricionais especificas de cada fase, a utilização de práticas rudimentares desencadeadoras de estresse, com conseguinte liberação de cortisol e, a pouca valorização da equipe de colaboradores, fatos estes, em diferentes níveis, têm contribuído na obtenção de resultados pouco desejáveis. Maior atenção carece ser dada na organização de toda cadeia produtiva e em especial, das unidades de produção de pequenos ruminantes.

Artificial Insemination is one of the most used animal reproduction biotechniques in small ruminants, contributing to the incessant search for animal improvement in this species and presenting itself as viable to small, medium and large producers. By the conventional method, with estrus observation, or with the use of estrus synchronization and ovulation induction protocols, using Fixed Time Artificial Insemination (IATF), there are several challenges encountered when executing a commercial program. Understanding the non-standardization of management in the various production units, as well as the little or no attention given, by most producers, to animal welfare issues, involving thermal comfort, meeting the specific nutritional needs of each phase, the use rudimentary practices that trigger stress, with the consequent release of cortisol and, the low appreciation of the team of employees, facts that, at different levels, have contributed to the achievement of undesirable results. Greater attention needs to be paid to the organization of the entire production chain and, in particular, of small ruminant production units.

Effectiveness of near-infrared spectroscopy as a non-invasive tool to discriminate spectral profiles of in vitro cultured oocytes from goats

Here, we aimed to discriminate between the spectral profiles of spent culture media after oocyte in vitro maturation (IVM) and culture (IVC) from goats of different ages subjected to repeated hormonal treatments. The profiles were discriminated using near infrared (NIR) spectroscopy combined with multivariate methods. A total of 19 goats (young = 10; old = 9) were subjected to serial hormonal stimulation (HS) with gonadotropins. Cumulus oophorus complexes (COCs) were collected using laparoscopic ovum pick-up (LOPU) and subjected to IVM and parthenogenetic activation. The initial embryos were subjected to IVC. Spent culture media were collected after oocyte IVM and on day 2 of IVC and analyzed using NIR spectroscopy. NIR spectral data were interpreted through chemometric methods, such as principle component analysis (PCA) and partial least square discriminant analysis (PLS-DA). The results of PCA analysis clearly showed a separation in the spectral profiles between the experimental groups (HS sessions; young and old animals) both after IVM and IVC. Overall, the main absorption bands were attributed to the C-H group second overtone, first overtone of O-H and N-H, and C-H combinations and may serve as molecular markers. On the other hand, the spectral data obtained using PLS-DA models provided a better classification of the groups. The results showed the possibility of discriminating young and old groups as well as the three HS sessions with high specificity, sensitivity, and accuracy using NIR spectra. Thus, the culture medium analysis using NIR spectroscopy combined with multivariate methods indicated the dissimilarities between the groups and provided an insight into the in vitro development of goat oocytes. This technique serves as an efficient, objective, rapid, and non-invasive method to discriminate spectral profiles.(AU)