Effects of magnesium sulfate and dioctyl sodium sulfosuccinate on fecal hydration, output and systemic hydration in healthy horses / [Efeitos do sulfato de magnésio e do dioctil-sulfossuccinato de sódio na hidratação e eliminação fecal e sobre a hidratação sistêmica de cavalos saudáveis]

Magnesium sulphate (MS) and dioctyl sodium sulphosuccinate (DSS) are laxative drugs frequently used for the treatment of impactions. The aim of this study was to compare the effects of MS and DSS in fecal hydration, output and systemic hydration in healthy horses. Five healthy horses received 3 treatments with a 21-day interval. Treatment 1 was performed with administration of 4 liters of warm water; treatment 2: administration of 4 liters of warm water associated with 1g/kg of MS; and treatment 3: administration of 4 liters of warm water associated with 20mg/kg DSS. General and specific physical examination of the digestive system were performed, alongside with packed cell volume and total plasma protein measurement, abdominal ultrasonography and quantification of the amount and hydration of feces, before and 6, 12, 24, 36 and 48 hours after the treatments. No adverse effects were observed. The administration of laxatives promoted greater fecal output and hydration without resulting in systemic dehydration, yet no differences were observed between treatments. The absence of adverse effects of DSS demonstrates the safety of its use as a laxative drug at a dose of 20mg/kg. Studies comparing the effects of the laxative drugs in horses with large colon impaction are needed.(AU)

O sulfato de magnésio (SM) e o dioctil-sulfossuccinato de sódio (DSS) são drogas laxativas frequentemente utilizadas no tratamento da compactação. O objetivo deste estudo foi comparar os efeitos do SM e do DSS na hidratação e eliminação fecal, e na hidratação sistêmica de cavalos saudáveis. Cinco cavalos receberam três tratamentos, com intervalo de 21 dias. O tratamento 1 consistiu na administração de quatro litros de água morna; o tratamento 2: administração de quatro litros de água morna associada a 1g/kg de SM; e o tratamento 3: administração de quatro litros de água morna associada a 20mg/kg de DSS. Foram realizados exames físicos gerais e específicos do trato digestivo, hematócrito e proteína plasmática total, ultrassonografia abdominal e mensuração da quantidade e hidratação das fezes antes e após seis, 12, 24, 36 e 48 horas dos tratamentos. Não foram observados efeitos adversos. A administração de ambos os laxantes promoveu maior produção fecal e hidratação, sem resultar em desidratação sistêmica, entretanto não houve diferença entre os tratamentos. A ausência de efeitos adversos do DSS demonstra a segurança de seu uso como medicamento laxante na dose de 20mg/kg. São necessários estudos que comparem o efeito dos laxantes em equinos portadores de compactação de cólon.(AU)

Typhlocolitis by Edwardsiella tarda in a cow

Background: Edwardsiella tarda has been report as etiology of gastroenteritis in both human and veterinary medicine,usually associated with exposure to aquatic environments in immunocompromised individuals. The present report describesa case of typhlocolitis in a cow in the west region of Santa Catarina state, Brazil.Case: After clinically evaluation of animal and proceeding euthanasia and realized the necropsy. Tissue samples were collected, and routinely processed for histological examination. Histopathological lesions were described as mild, moderateand severe. Tissue and swabs samples of small and large intestine were sent to standard microbiological culture processing.At necropsy, cattle presented severe dehydration and emaciation. Eye and vaginal mucosa were severely congested. Theopening of the abdominal cavity revealed a great amount of greenish fluid and a large amount of fecal material, associatedwith diffuse severe peritonitis, evidenced by hyperemia and severe deposition of fibrin in the visceral and parietal peritonealsurface. In the serosa of the cecum, there were two points of rupture, observed in the proximal colon. The mucosa of cecumand colon were severely edematous, hyperemic, and presented diffusely distributed pinpoint round hemorrhages, as wellas fibrinonecrotic material adhered to the surface. Histologically, in the mucosa of cecum and colon, moderate to severediffuse inflammatory infiltrate of neutrophils, lymphocytes and plasma cells associated with multifocal severe necrosiswere observed. Moderate diffuse fibrin deposition was evidenced in the submucosa and muscular, as well as multifocalmoderate necrosis in the muscular layer. In the serous, severe diffuse inflammatory infiltrate of neutrophils associated withfibrin deposition and innumerous coccoid bacterial colonies were observed. The samples subjected to bacterial isolationshowed growth of Edwardsiella tarda. All samples were negative for Salmonella spp. and...

Typhlocolitis by Edwardsiella tarda in a cow

Background: Edwardsiella tarda has been report as etiology of gastroenteritis in both human and veterinary medicine,usually associated with exposure to aquatic environments in immunocompromised individuals. The present report describesa case of typhlocolitis in a cow in the west region of Santa Catarina state, Brazil.Case: After clinically evaluation of animal and proceeding euthanasia and realized the necropsy. Tissue samples were collected, and routinely processed for histological examination. Histopathological lesions were described as mild, moderateand severe. Tissue and swabs samples of small and large intestine were sent to standard microbiological culture processing.At necropsy, cattle presented severe dehydration and emaciation. Eye and vaginal mucosa were severely congested. Theopening of the abdominal cavity revealed a great amount of greenish fluid and a large amount of fecal material, associatedwith diffuse severe peritonitis, evidenced by hyperemia and severe deposition of fibrin in the visceral and parietal peritonealsurface. In the serosa of the cecum, there were two points of rupture, observed in the proximal colon. The mucosa of cecumand colon were severely edematous, hyperemic, and presented diffusely distributed pinpoint round hemorrhages, as wellas fibrinonecrotic material adhered to the surface. Histologically, in the mucosa of cecum and colon, moderate to severediffuse inflammatory infiltrate of neutrophils, lymphocytes and plasma cells associated with multifocal severe necrosiswere observed. Moderate diffuse fibrin deposition was evidenced in the submucosa and muscular, as well as multifocalmoderate necrosis in the muscular layer. In the serous, severe diffuse inflammatory infiltrate of neutrophils associated withfibrin deposition and innumerous coccoid bacterial colonies were observed. The samples subjected to bacterial isolationshowed growth of Edwardsiella tarda. All samples were negative for Salmonella spp. and...(AU)

Colonic epithelial nodular hyperplasia associated with strongyloidiasis in cats in the Amazon region, Pará State, Brazil / Hiperplasia epitelial nodular no cólon de felinos associada à estrongiloidíase na região Amazônica, Pará, Brasil

Strongyloides spp. are intestinal parasites that affect several animal species. Four species of the genus have been reported in domestic cats: S. felis, S. planiceps, S.stercoralis and S. tumefaciens . Reports describing infection by these nematodes in domestic cats in Brazil are scarce. This study aimed to describe the pathological features of Strongyloides spp. parasitism in two cats in the Amazon region, state of Pará, Brazil. During the necropsy of the two cats, numerous whitish nodules approximately 0.2cm in diameter were observed in the wall of the large intestine. The nodules were conspicuous in the colonic mucosa and exhibited a punctate aperture facing the center of the lumen. Microscopically, these nodules were formed by projections of the mucosal epithelium into the submucosa, which formed tubules lined with a single layer of columnar epithelium, with high cellularity and rare goblet cells, characterizing epithelial hyperplasia of the crypts. Adult nematodes and eggs observed in the lumen of the tubules were morphologically compatible with Strongyloides spp. Numerous larvae were also observed in the interstitium adjacent to the nodule. A mild lymphocytic infiltrate was observed neighboring the hyperplastic nodules. The histological changes are consistent with those described for S. tumefaciens infection.

Strongyloides spp. são helmintos que podem parasitar o intestino de diversas espécies animais. Quatro espécies desse gênero têm sido descritas em felinos domésticos: S. felis, S. planiceps, S. stercoralis e S. tumefaciens. Trabalhos sobre o parasitismo por estrongiloides em gatos domésticos no Brasil são escassos. O objetivo deste trabalho é descrever os aspectos patológicos do parasitismo por Strongyloides spp. em dois gatos da região amazônica, no estado do Pará. Estes apresentavam numerosos nódulos esbranquiçados no intestino grosso, medindo cerca de 0,2 centímetros de diâmetro, que na microscopia eram constituídos por projeções do epitélio hiperplásico da mucosa para submucosa, formando túbulos contendo várias fêmeas adultas, ovos e larvas com morfologia compatível com a de Strongyloides spp. e margeados por larvas e remanescentes do aglomerado linfoide. As alterações histológicas foram compatíveis com as descritas no parasitismo por S. tumefaciens.

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

Purpose: To evaluate the inflammatory intensity and measure the tissue content of the proteins claudin-3 and occludin in the colonic mucosa without fecal stream submit to intervention with curcumin. Methods: Thirty-six rats were submitted to a proximal colostomy and a distal mucous fistula and divided into two groups according to sacrifice to be performed two or four weeks. Each group was divided into three subgroups according daily application of enemas containing saline, curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Claudin-3 and occludin were determined by immunohistochemistry. The tissue content of claudin-3 and occludin were quantified by computer-assisted image analysis. Mann-Whitney, Student t and ANOVA tests were used to analyze the results establishing the level of significance of 5% for both (p 0.05). Results: Curcumin at both concentrations reduces the inflammation and preserves the tissue content of the proteins claudin-3 and occludin, which was related to the concentration used and to the time of the intervention. Conclusion: The application of enemas with curcumin reduces inflammation and preserves the tissue content of the proteins claudin-3 and occludin in the colonic mucosa devoid from the fecal stream.(AU)

Colonic epithelial nodular hyperplasia associated with strongyloidiasis in cats in the Amazon region, Pará State, Brazil / Hiperplasia epitelial nodular no cólon de felinos associada à estrongiloidíase na região Amazônica, Pará, Brasil

Strongyloides spp. are intestinal parasites that affect several animal species. Four species of the genus have been reported in domestic cats: S. felis, S. planiceps, S.stercoralis and S. tumefaciens . Reports describing infection by these nematodes in domestic cats in Brazil are scarce. This study aimed to describe the pathological features of Strongyloides spp. parasitism in two cats in the Amazon region, state of Pará, Brazil. During the necropsy of the two cats, numerous whitish nodules approximately 0.2cm in diameter were observed in the wall of the large intestine. The nodules were conspicuous in the colonic mucosa and exhibited a punctate aperture facing the center of the lumen. Microscopically, these nodules were formed by projections of the mucosal epithelium into the submucosa, which formed tubules lined with a single layer of columnar epithelium, with high cellularity and rare goblet cells, characterizing epithelial hyperplasia of the crypts. Adult nematodes and eggs observed in the lumen of the tubules were morphologically compatible with Strongyloides spp. Numerous larvae were also observed in the interstitium adjacent to the nodule. A mild lymphocytic infiltrate was observed neighboring the hyperplastic nodules. The histological changes are consistent with those described for S. tumefaciens infection.(AU)

Strongyloides spp. são helmintos que podem parasitar o intestino de diversas espécies animais. Quatro espécies desse gênero têm sido descritas em felinos domésticos: S. felis, S. planiceps, S. stercoralis e S. tumefaciens. Trabalhos sobre o parasitismo por estrongiloides em gatos domésticos no Brasil são escassos. O objetivo deste trabalho é descrever os aspectos patológicos do parasitismo por Strongyloides spp. em dois gatos da região amazônica, no estado do Pará. Estes apresentavam numerosos nódulos esbranquiçados no intestino grosso, medindo cerca de 0,2 centímetros de diâmetro, que na microscopia eram constituídos por projeções do epitélio hiperplásico da mucosa para submucosa, formando túbulos contendo várias fêmeas adultas, ovos e larvas com morfologia compatível com a de Strongyloides spp. e margeados por larvas e remanescentes do aglomerado linfoide. As alterações histológicas foram compatíveis com as descritas no parasitismo por S. tumefaciens.(AU)

Primary muscle hypertrophy of the colon in a cat: a rare presentation

In animals and humans, intestinal muscular hypertrophy has only been observed in the small intestine, and this appears to be the first report of the disease affecting the large intestine. A rare case of primary muscle hypertrophy of the colon was found during the necropsy of a male, 3-year-old, mixed breed cat that died due to poisoning by carbamate. The necropsy revealed that the entire colon had marked circumferential thickening with narrowing of the lumen. Microscopically, diffuse intense smooth muscle hypertrophy was observed, especially of the inner circular muscle layer and muscularis mucosa. Those finds were associated with moderate multifocal to coalescing lymphocytic inflammatory infiltrate. Smooth muscle fibers of the large and small intestine showed no CDC47 expression and the percentage of nuclei in the muscle layers was similar between the colon and the duodenum. Based on macroscopic, microscopic and immunohistochemical findings, the diagnosis of diffuse primary muscle hypertrophy of the colon was confirmed.

Primary muscle hypertrophy of the colon in a cat: a rare presentation

In animals and humans, intestinal muscular hypertrophy has only been observed in the small intestine, and this appears to be the first report of the disease affecting the large intestine. A rare case of primary muscle hypertrophy of the colon was found during the necropsy of a male, 3-year-old, mixed breed cat that died due to poisoning by carbamate. The necropsy revealed that the entire colon had marked circumferential thickening with narrowing of the lumen. Microscopically, diffuse intense smooth muscle hypertrophy was observed, especially of the inner circular muscle layer and muscularis mucosa. Those finds were associated with moderate multifocal to coalescing lymphocytic inflammatory infiltrate. Smooth muscle fibers of the large and small intestine showed no CDC47 expression and the percentage of nuclei in the muscle layers was similar between the colon and the duodenum. Based on macroscopic, microscopic and immunohistochemical findings, the diagnosis of diffuse primary muscle hypertrophy of the colon was confirmed.(AU)

Secondary bile acids effects in colon pathology. Experimental mice study

To assess whether deoxycholic acid (DOC) and lithocholic acid (LCA) administered in a period of six months in a concentration of 0.25% may have a carcinogenic role in mice colon. The study used C57BL6 female mice divided into four groups. The control group received a balanced diet and the others received diets supplemented with 0.25% DOC, 0.25% LCA and 0.125% DOC+0.125% LCA, respectively. After euthanasia, the lesions found in the resected gastrointestinal tracts were stained with hematoxylin-eosin and examined microscopically. No gastrointestinal tract changes were observed in the control group, while hyperplastic Peyer's patches in the small intestine, flat adenomas with mild dysplasia and chronic colitis at the level of the colon were found in all three test groups. The colonic lesions prevailed in the proximal colon. The highest number of flat adenoma lesions (8), hyperplasia of Peyer's patches (25) and chronic colitis (2) were found in mice fed with diet and LCA. Precancerous or cancerous pathological lesions could not be identified. Instead, adenomatous colonic injuries occurred in a shorter period of time (six months), compared to the reported data.(AU)

Infiltrative Lipomatosis in the Neck and Colon of a Spanish Pure Breed Horse

The aim of this study was to report a case of infiltrative lipomatosis in the neck and colon in a horse. A 12-year-old male Spanish Pure Breed Horse with deformation of dorsal neck, weighing 650 kg, with a history of acute colic for approximately 24 hours. Euthanasia was undertaken. A necropsy revealed general excessive lipid accumulation in subcutaneous tissue in the dorsal neck. The pelvic flexure of the large colon was found to be displaced into the right cranial abdomen. A large multinodular and multifocal infiltrative fatty mass coalescing was present in a 1.5 m segment of colon with severed lipomatosis; the small intestine proximal to this lesion was moderately distended with fluid. Lipomatosis in the dorsal neck was observed, characterized by diffuse thickening of the subcutis by mature adipose tissue. In conclusion we report a case of severed infiltrative lipomatosis intestinal in large colon and neck.

Infiltrative Lipomatosis in the Neck and Colon of a Spanish Pure Breed Horse

The aim of this study was to report a case of infiltrative lipomatosis in the neck and colon in a horse. A 12-year-old male Spanish Pure Breed Horse with deformation of dorsal neck, weighing 650 kg, with a history of acute colic for approximately 24 hours. Euthanasia was undertaken. A necropsy revealed general excessive lipid accumulation in subcutaneous tissue in the dorsal neck. The pelvic flexure of the large colon was found to be displaced into the right cranial abdomen. A large multinodular and multifocal infiltrative fatty mass coalescing was present in a 1.5 m segment of colon with severed lipomatosis; the small intestine proximal to this lesion was moderately distended with fluid. Lipomatosis in the dorsal neck was observed, characterized by diffuse thickening of the subcutis by mature adipose tissue. In conclusion we report a case of severed infiltrative lipomatosis intestinal in large colon and neck.(AU)

Casuística dos casos de cólica em equinos atendidos no Centro Integrado de Tratamento Equino, município de Paudalho, Pernambuco, no período de janeiro de 2012 a outubro de 2015 / Casuistry of colic cases in horses attended at Centro Integrado de Tratamento Equino, Paudalho municipality, Pernambuco, from january 2012 to october 2015 / Casuística de casos de cólicos en caballos atendidos en el Centro Integrado de Tratamento Equino, municipio Paudalho, Pernambuco, de enero de 2012 a octubre de 2015

A síndrome cólica é uma das patologias clínicas mais importantes e desafiantes na medicina equina, não só pela sua prevalência, mas também pelas consequências que pode implicar. Esta pode ter relação com diversos fatores. Objetivou-se com esse trabalho realizar um estudo casuístico dos casos clínico-cirúrgicos de cólica em equídeos atendidos no Centro Integrado de Tratamento Equino (CITEQUIN), localizado no município de Paudalho/PE, no período de janeiro de 2012 a outubro de 2015. Pode-se constatar que de 387 casos atendidos no CITEQUIN, 260 casos foram atendimentos de cólica clínica com 15 óbitos relatados, contra 121 casos de cólica cirúrgica, em que 42 animais foram a óbito. A maior ocorrência dos casos de cólica cirúrgica foi em decorrência de compactação e deslocamento de cólon maior, somando 31,74% dos 63 casos que obtiveram diferenciação dos tipos de cólica descritas nas fichas clínicas pertencentes ao CITEQUIN.

Colic syndrome is one of the most important and challenging clinical conditions in equine medicine, for not only its prevalence, but also the consequences that may entail. This syndrome can be related to several factors. The objective of this work is to carry out a casuistic study of the clinical and surgical cases of colic in horses treated at the Centro Integrado de Tratamento Equino, located in the municipality of Paudalho/PE in the period from January 2012 to October 2015. In this study, it was found that of 387 cases treated at the CITEQUIN, 260 cases were clinical colic with 15 reported deaths, compared to 121 cases of surgical colic, where 42 animals died. The higher incidence of surgical colic cases were due to compression and large colon displacement, totaling 31.74% of the 63 cases that obtained differentiation of the types of colic described in the clinical record belonging to CITEQUIN.

El síndrome de cólico es una de las condiciones clínicas más importantes y desafiantes en la medicina equina, no solo por su prevalencia, sino también por las consecuencias que esto puede acarrear. Este síndrome puede estar relacionado con varios factores. El objetivo de este trabajo es realizar un estudio casuístico de los casos clínicos y quirúrgicos de cólico en caballos tratados en el Centro Integrado de Tratamiento Equino, ubicado en el municipio de Paudalho/PE en el período de enero de 2012 a octubre de 2015. En este estudio, se encontró que de 387 casos tratados en el CITEQUIN, 260 casos fueron cólicos clínicos con 15 muertes informadas, en comparación con 121 casos de cólico quirúrgico, donde 42 animales murieron. La mayor incidencia de casos de cólicos quirúrgicos se debió a compresión y desplazamiento de colon grande, totalizando el 31,74% de los 63 casos que obtuvieron diferenciación de los tipos de cólicos descritos en el registro clínico perteneciente a CITEQUIN.

Casuística dos casos de cólica em equinos atendidos no Centro Integrado de Tratamento Equino, município de Paudalho, Pernambuco, no período de janeiro de 2012 a outubro de 2015 / Casuistry of colic cases in horses attended at Centro Integrado de Tratamento Equino, Paudalho municipality, Pernambuco, from january 2012 to october 2015 / Casuística de casos de cólicos en caballos atendidos en el Centro Integrado de Tratamento Equino, municipio Paudalho, Pernambuco, de enero de 2012 a octubre de 2015

A síndrome cólica é uma das patologias clínicas mais importantes e desafiantes na medicina equina, não só pela sua prevalência, mas também pelas consequências que pode implicar. Esta pode ter relação com diversos fatores. Objetivou-se com esse trabalho realizar um estudo casuístico dos casos clínico-cirúrgicos de cólica em equídeos atendidos no Centro Integrado de Tratamento Equino (CITEQUIN), localizado no município de Paudalho/PE, no período de janeiro de 2012 a outubro de 2015. Pode-se constatar que de 387 casos atendidos no CITEQUIN, 260 casos foram atendimentos de cólica clínica com 15 óbitos relatados, contra 121 casos de cólica cirúrgica, em que 42 animais foram a óbito. A maior ocorrência dos casos de cólica cirúrgica foi em decorrência de compactação e deslocamento de cólon maior, somando 31,74% dos 63 casos que obtiveram diferenciação dos tipos de cólica descritas nas fichas clínicas pertencentes ao CITEQUIN.(AU)

Colic syndrome is one of the most important and challenging clinical conditions in equine medicine, for not only its prevalence, but also the consequences that may entail. This syndrome can be related to several factors. The objective of this work is to carry out a casuistic study of the clinical and surgical cases of colic in horses treated at the Centro Integrado de Tratamento Equino, located in the municipality of Paudalho/PE in the period from January 2012 to October 2015. In this study, it was found that of 387 cases treated at the CITEQUIN, 260 cases were clinical colic with 15 reported deaths, compared to 121 cases of surgical colic, where 42 animals died. The higher incidence of surgical colic cases were due to compression and large colon displacement, totaling 31.74% of the 63 cases that obtained differentiation of the types of colic described in the clinical record belonging to CITEQUIN.(AU)

El síndrome de cólico es una de las condiciones clínicas más importantes y desafiantes en la medicina equina, no solo por su prevalencia, sino también por las consecuencias que esto puede acarrear. Este síndrome puede estar relacionado con varios factores. El objetivo de este trabajo es realizar un estudio casuístico de los casos clínicos y quirúrgicos de cólico en caballos tratados en el Centro Integrado de Tratamiento Equino, ubicado en el municipio de Paudalho/PE en el período de enero de 2012 a octubre de 2015. En este estudio, se encontró que de 387 casos tratados en el CITEQUIN, 260 casos fueron cólicos clínicos con 15 muertes informadas, en comparación con 121 casos de cólico quirúrgico, donde 42 animales murieron. La mayor incidencia de casos de cólicos quirúrgicos se debió a compresión y desplazamiento de colon grande, totalizando el 31,74% de los 63 casos que obtuvieron diferenciación de los tipos de cólicos descritos en el registro clínico perteneciente a CITEQUIN.(AU)

Encarceramento em forame epiplóico em equino: relato de caso / The epiploic incarceration in horses: case report / Encarcelamiento en forame epiplóico en equinos: estúdio de caso

O encarceramento em forame epiplóico em equinos é observado em 2-10% das cólicas encaminhadas para a cirurgia, causando um comprometimento vascular que pode levar a necessidade de ressecção do segmento de alça intestinal afetado ou, até mesmo, eutanásia do animal. Um equino, macho, castrado, 400 kg, 5 anos de idade, da raça Mangalarga foi admitido com sinais de cólica, que tinha duração de 24 horas. Após os exames pertinentes, foi indicada a cirurgia, a qual foi negada pelo proprietário. Após tentativa de manutenção da vida do animal, foi indicada a eutanásia, sendo diagnosticado encarceramento de íleo no forame epiplóico na necropsia. Foi avaliado que o animal possuiria um bom prognóstico caso a laparotomia fosse realizada.

The epiploic incarceration in horses is observed in 2-10% of the cramps referred for surgery, causing vascular impairment that may lead to the need for resection of the affected intestinal segment or even euthanasia of the animal. A horse, 400 kg, male, 5-year-old, Mangalarga was admitted with signs of colic, which lasted 24 hours. After the pertinent examinations, the surgery was indicated, which was denied by the owner. After trying to maintain the life of the animal, euthanasia was indicated, being diagnosed incarceration of ileus in the epiploic foramen at necropsy. It was evaluated that the animal would have a good prognosis if the laparotomy was performed.

El encarcelamiento en foramen epiplóico en equinos se observa en el 2-10% de los cólicos derivados a la cirugía, generando un compromiso vascular que puede llevar a la necesidad de resección del segmiento de las asas intestinales afectadas o, y incluso, la eutanasia del animal. Un equino, macho, castrado, 400 kg, 5 anos de edad, de la raza Mangalarga fue admitido con seriales de cólico, que tenía una duración de 24 horas. Después de los exámenes pertinentes, fue indicada la cirugía, la cual fue negada por el propietario. Después de un intento de mantenimiento de la vida del animal, fue indicada la eutanasia, siendo diagnosticado encarcelamiento de íleo en el foramen epiplóico en la necropsia. Se evaluó que el animal tendría un buen pronóstico si se realizaba la laparotomía.

Encarceramento em forame epiplóico em equino: relato de caso / The epiploic incarceration in horses: case report / Encarcelamiento en forame epiplóico en equinos: estúdio de caso

O encarceramento em forame epiplóico em equinos é observado em 2-10% das cólicas encaminhadas para a cirurgia, causando um comprometimento vascular que pode levar a necessidade de ressecção do segmento de alça intestinal afetado ou, até mesmo, eutanásia do animal. Um equino, macho, castrado, 400 kg, 5 anos de idade, da raça Mangalarga foi admitido com sinais de cólica, que tinha duração de 24 horas. Após os exames pertinentes, foi indicada a cirurgia, a qual foi negada pelo proprietário. Após tentativa de manutenção da vida do animal, foi indicada a eutanásia, sendo diagnosticado encarceramento de íleo no forame epiplóico na necropsia. Foi avaliado que o animal possuiria um bom prognóstico caso a laparotomia fosse realizada.(AU)

The epiploic incarceration in horses is observed in 2-10% of the cramps referred for surgery, causing vascular impairment that may lead to the need for resection of the affected intestinal segment or even euthanasia of the animal. A horse, 400 kg, male, 5-year-old, Mangalarga was admitted with signs of colic, which lasted 24 hours. After the pertinent examinations, the surgery was indicated, which was denied by the owner. After trying to maintain the life of the animal, euthanasia was indicated, being diagnosed incarceration of ileus in the epiploic foramen at necropsy. It was evaluated that the animal would have a good prognosis if the laparotomy was performed.(AU)

El encarcelamiento en foramen epiplóico en equinos se observa en el 2-10% de los cólicos derivados a la cirugía, generando un compromiso vascular que puede llevar a la necesidad de resección del segmiento de las asas intestinales afectadas o, y incluso, la eutanasia del animal. Un equino, macho, castrado, 400 kg, 5 anos de edad, de la raza Mangalarga fue admitido con seriales de cólico, que tenía una duración de 24 horas. Después de los exámenes pertinentes, fue indicada la cirugía, la cual fue negada por el propietario. Después de un intento de mantenimiento de la vida del animal, fue indicada la eutanasia, siendo diagnosticado encarcelamiento de íleo en el foramen epiplóico en la necropsia. Se evaluó que el animal tendría un buen pronóstico si se realizaba la laparotomía.(AU)

Comparative study of the hyperbaric hyperoxygenation in ischemic colonic loops in rats / Estudo comparativo da hiperoxigenação hiperbárica em alças cólicas isquêmicas em ratos

PURPOSE: To analyze and to evaluate the effect of the hyperbaric hyperoxygenation in the mechanical resistance of ischemic colon of rats. METHODS: Eighty rats, distributed in four groups of 20 animals in each one, were used. In group 1 (G1), the control group, ischemia was not caused. Group 2 was submitted to the lesser degree of ischemia. Group 3 was submitted to the intermediate degree of ischemia. In group 4, a bigger degree of intestinal ischemia was provoked. Each group was divided in two sub-groups of ten animals each: with hyperbaric chamber (CC) and without hyperbaric chamber (SC). The animals of the four CC subgroups were placed in an experimental hyperbaric chamber in order to inhale oxygen at 100 percent, at two Absolute Atmospheres, for 120 minutes, for a four-day consecutive period. The animals of the four SC subgroups were kept in environment air during the five days of the experiment. All animals have been submitted to the mechanical study of the intestinal loop by the pressure test of the rupture by liquid distension. The euthanasia occurred in the fifth post-operative day. RESULTS: Considering the ischemia factor, the four groups were different among them (p=0.0001). There was no statistical difference between subgroups CC and SC (p=0.3461). CONCLUSION: The hyperbaric oxygen-therapy did not present improvement on the induced ischemia in rats upright colic loop.(AU)

OBJETIVO: Analisar e avaliar os efeitos da hiperoxigenação hiperbárica na resistência mecânica do cólon isquêmico de ratos. MÉTODOS: Foram utilizados 80 ratos distribuídos em quatro grupos de 20 animais. No grupo 1, grupo controle, não se provocou isquemia. O grupo 2 foi submetido ao grau menor de isquemia. O grupo 3 foi submetido ao grau intermediário de isquemia. No grupo 4, provocou-se grau maior de isquemia intestinal. Cada grupo foi dividido em dois subgrupos de dez animais cada: com câmara hiperbárica (CC) e sem câmara hiperbárica (SC). Os animais dos quatro subgrupos CC foram colocados em uma câmara hiperbárica experimental para inalarem oxigênio a 100 por cento, a duas atmosferas absolutas, durante 120 minutos, por quatro dias consecutivos. Os animais dos quatro subgrupos SC foram mantidos somente em ar ambiente. Todos animais foram submetidos ao estudo mecânico da alça intestinal pelo teste de pressão de ruptura pela distensão por líquido. A eutanásia ocorreu no quinto dia pós-operatório. RESULTADOS: Os quatro grupos são diferentes entre si considerando o fator isquemia (p= 0.0001). Não houve diferença estatística entre os subgrupos CC e SC (p= 0.3461). CONCLUSÃO: A oxigenoterapia hiperbárica não apresentou melhora sobre a isquemia induzida em alça cólica íntegra de ratos.(AU)

Ação do extrato de Jatropha gossypiifolia L. (pião roxo) na cicatrização de anastomose colônica: estudo experimental em ratos / Effects of Jatropha gossypiifolia L. (bellyache bush) extract on the healing process of colonic anastomosis: experimental study in rats

INTRODUÇÃO: A fitoterapia vem despertando crescente interesse na comunidade científica em relação às suas potenciais propriedades cicatriciais. Poucos são os estudos com metodologia científica existentes na literatura, constituindo-se assim um campo aberto para novas investigações. OBJETIVOS: Analisar a ação da Jatropha gossypiifolia L. (pião roxo) na cicatrização de anastomose colônica em ratos. MÉTODOS: Quarenta ratos Wistar foram submetidos à secção de toda a circunferência do cólon, sendo logo anastomosada com oito pontos separados usando fio monofilamentar de polipropileno 6-0. Aleatoriamente, os animais foram distribuídos em grupo controle (GC), contendo 20 ratos, nos quais foi administrada solução de cloreto de sódio a 0,9% por via intraperitoneal (1ml/kg) e grupo Jatropha (GJ), também com 20 nos quais foi administrado o extrato hidroalcoólico de Jatropha gossypiifolia L. por via intraperitoneal (1ml/kg). Cada um dos grupos foi subdividido em dois subgrupos de dez animais de acordo com a data de sacrifício, três e sete dias (GC3/ GC7 e GJ3/GJ7). Após o sacrifício foi retirada parte do cólon compreendendo 1 cm acima e abaixo da anastomose e submetido ao teste de resistência à insuflação de ar atmosférico. A seguir, foi aberta e retirada uma porção de 1,0 x 0,5cm de tamanho colocada em formol a 10% para análise histológica, na qual foram usadas a coloração de HematoxilinaûEosina e o Tricrômico de Masson. RESULTADOS: Na avaliação do teste de pressão de ruptura entre os grupos houve significância estatística quando avaliados em relação à data de sacrifício Assim no terceiro dia a média no GC (25,4 mmHg.) e no GJ (76,4 mmHg.) estabeleceu p = 0.013, e no sétimo dia a média no GC (187,3 mmHg.) e no GJ (135,1 mmHg.) estabeleceu p = 0.014. Ao analisar as variáveis microscópicas entre os grupos no 3º dia, somente não houve diferença significativa nas variáveis edema e polimorfonucleares. Já no 7º dia todas as variáveis analisadas apresentaram diferença...(AU)

INTRODUCTION: Phytotherapy has been drawing interest from the scientific community regarding its potential wound healing properties. There are few studies available that present a correct scientific methodology and, therefore, phytotherapy remains an open field for further research. PURPOSE: To evaluate the effects of Jatropha gossypiifolia L. (bellyache bush) on the healing of colonic anastomosis in rats. METHODS: Forty Wistar rats were subjected to a complete section of the colon, that was soon sutured with eight separate stitches using monofilament polipropilene 6-0. Randomly, the animals were divided into control group (CG), with 20 rats, which were injected with 0.9% sodium chloride solution intraperitoneally (1mL/kg) and Jatropha group (JG), also with 20 rats, which were injected with Jatropha gossypiifolia L. alcoholic extract intraperitoneally (1mL/kg). Each of the groups was subdivided into two subgroups of ten rats, according to the sacrifice date, third and seventh days (CG3/ CG7 and JG3/ JG7). After sacrifice, the segment of the colon containing 1cm proximal and distal to the site of anastomosis was removed and subjected to the pressure test with air insuflation. This segment was then opened and a 1.0 x 0.5cm part was removed and placed under 10% formaldehyde for histological analysis with hematoxylin-eosin and Masson's trichrome stainings. RESULTS: The evaluation of the pressure rupture test demonstrated statistical significance regarding the sacrifice date. On the third day, the average pressure of the CG (25,4mmHg) and the JG (76,4mHg) revealed p = 0.013, and on the seventh day, the average pressure of the CG (187,3 mmHg.) and the JG (135,1 mmHg.) revealed p = 0.014. When assessing the microscopic variables among the groups of the 3rd day, only the variables edema and polymorphonuclear cells did not show statistical differences. On the 7th day, all of the variables assessed demonstrated significant differences...(AU)

Extrato de Passiflora edulis na cicatrização de feridas cutâneas abertas em ratos: estudo morfológico e histológico / Extract from Passiflora edulis on the healing of open wounds in rats: morphometric and histological study

INTRODUÇÃO: Nas regiões interioranas do Brasil o cataplasma feito com folhas de Passiflora edulis tem sido usado pela população como cicatrizante, para tratar infecções e inflamações cutâneas, sem a comprovação científica dos seus potenciais benefícios. OBJETIVO: Avaliar a cicatrização de feridas abertas em ratos com a aplicação tópica de extrato hidroalcoólico de Passiflora edulis. MÉTODOS: Foram utilizados 60 ratos da linhagem Wistar, machos, adultos, divididos em dois grupos: o grupo Passiflora e o grupo Controle. Os animais do primeiro grupo foram tratados com o extrato de Passiflora edulis, e os do segundo grupo, com água destilada. A aplicação diária do extrato e da água destilada foi realizada sobre ferida circular padronizada de 2cm de diâmetro na região dorsal de cada animal. A avaliação da ferida foi feita do ponto de vista macro e microscópico nos períodos pré-determinados (7º, 14º e 21º dias). Fez-se a análise macroscópica da evolução do aspecto da lesão e medida da retração cicatricial da ferida, por planimetria digital. O estudo histológico em lâminas coradas pela Hematoxilina-Eosina e Tricômico de Masson considerou os parâmetros de elementos celulares inflamatórios incluindo colagenização e reepitelização. RESULTADOS: Não houve diferença estatisticamente significante entre as áreas cirúrgicas das feridas tratadas com Passiflora edulis e o grupo Controle; entretanto, histologicamente houve colagenização significantemente maior no 14º dia de pós-operatório nos animais do grupo Passiflora (p = 0,012). CONCLUSÕES: O uso tópico do extrato de Passiflora edulis não apresenta macroscopicamente efeito significativo na cicatrização de feridas na pele de ratos; entretanto microscopicamente apresenta aumento da proliferação fibroblástica no 7º dia e colagenização maior no 14º dia de pós-operatório.(AU)

INTRODUCTION: In Brazilian countryside, cataplasm made from Passiflora edulis leaves has been used by the population as a healing agent for infections and skin inflammations in an empiric basis. PURPOSE: the aim of this work was to evaluate the healing process of open wounds in rats, in which Passiflora edulis hydro-alcoholic extract was applied. METHODS: Sixty male, adult Wistar rats were divided into two groups: Passiflora group and Control group. Rats of the first group were treated with Passiflora edulis extract, and those of the second group received distilled water. The daily application of the extract or distilled water was carried out on a 2 cm diameter standardized circular wound on the dorsal region of each animal. Wound assessment was performed macroscopically and microscopically on the 7th, 14th, and 21st postoperative days. Microscopic analysis included hematoxylin-eosine and Masson Trichromium stains, evaluating inflammatory response, fibroplasia and collagen deposition. The wound retraction was evaluated by digital planimetry. RESULTS: No significant difference in the rate of wound healing was detected comparing both groups. However, a significant increase in the number of fibroblastic cells was seen on the 7th PO day, and significantly greater collagen deposition was observed on the 14th day PO day in rats from the Passiflora group (p=0,012). CONCLUSIONS: The application of the Passiflora edulis extract does not accelerate the healing process of open wounds in rats, but is associated with increased number of fibroblastic cells on 7th P.O. day and greater collagen deposition on the 14th PO day.(AU)

Extrato de Passiflora edulis na cicatrização de anastomose colônica em ratos: estudo morfológico e tensiométrico / Extract of Passiflora edulis in the healing of colonic anastomosis in rats: a tensiometric and morphologic study

INTRODUÇÃO: Pesquisas de novas substâncias com finalidades terapêuticas têm sido realizadas procurando isolar, extrair ou purificar novos compostos de origem vegetal. A Passiflora edulis (maracujá), espécie pertencente à família Passifloracea, originária das regiões tropicais e subtropicais do continente americano, é popularmente usada como sedativo, analgésico e antinflamatório e no tratamento de lesões cutâneas, feridas e erisipelas. OBJETIVO: Avaliar a cicatrização de anastomoses colônicas em ratos, que receberam extrato hidroalcoólico de Passiflora edulis no trans-operatório. MÉTODOS: Foram utilizados 40 ratos Wistar, distribuídos em dois grupos de 20 animais cada, denominados: grupo Passiflora (GP) e grupo controle (GC). Os ratos de cada grupo foram separados em dois subgrupos de 10 animais cada, avaliados no 3º e 7º dia do pós-operatório. O procedimento cirúrgico constou de secção da alça colônica esquerda, 5cm acima da reflexão peritoneal com preservação da arcada vascular e anastomose término-terminal em plano único. O grupo Passiflora recebeu dose única intraperitoneal do extrato hidroalcoólico de Passiflora edulis na dose de 250 mg/Kg. O grupo controle recebeu dose única de solução salina intraperitoneal em volume igual ao GP. Os parâmetros avaliados foram: aspectos macroscópicos da parede e cavidade abdominal, aderências perianastomóticas, pressão de ruptura à insuflação de ar, reação inflamatória tecidual da anastomose que constou de polimorfonucleares, monomorfonucleares e proliferação fibroblástica. RESULTADOS: Os aspectos macroscópicos não apresentaram diferenças significantes entre os grupos. Não ocorreu nenhuma deiscência de anastomose nos grupos estudados. Com relação à pressão de ruptura à insuflação de ar, observou-se que a média foi significantemente maior no subgrupo que recebeu o extrato de Passiflora no 3º dia (P3), cuja pressão foi 42,6 ± 17,8 mmHg em comparação ao subgrupo controle (C3), cuja pressão foi 25,4 ± 14,1 mmHg, p=0,028...(AU)

INTRODUCTION: Investigation of new substances with therapeutic effects have been done trying to isolate, extract or purify new compounds of vegetable origin. The Passiflora edulis (maracujá) species from the Plassifloracia family, originated from the tropical and subtropical regions of the american continent, is found all over Brazil. It is commonly used as a sedative, painkiller and anti-inflammatory drug and also for the treatment of skin wounds, lesions and Erisipelae. PURPOSE: To evaluate the wound healing in colonic anastomosis in rats that received an hydro-alcoholic extract of Passiflora edulis peri-operatively. METHOD: 40 wistar rats were used distributed into two groups of 20 rats each, named: Passiflora edulis group (GP) and control group (GC). The rats of each group were separated into two subgroups of 10 animals each and were evaluated on the 3rd and 7th postoperative days. The surgical procedure consisted of a section of the left colon, 5 cm above the peritoneal reflexion with preservation of the vascular elements. Intestinal continuity was restored by an end-to-end single layer anastomosis. The Passiflora edulis group received an intraperitoneal application of the hydro-alcoholic extract in the dosage of 250 mg/kg. The control-group received one intraperitoneal dose of a saline with the same volume of the GP. The parameters evaluated were: macroscopic aspects of the wall and abdominal cavity, perianastomotic (adherences), bursting pressure, inflammatory tissue reaction on the anastomotic wound. RESULTS: The macroscopic aspects did not differ between the groups. No rupture in the anastomotic wound was seen in any rat. Regarding the bursting pressure, it was noticed that the average pressure was significantly higher in the subgroup that received the Passiflora edulis extract on the 3rd day (P3) (42,6 ± 17,8 mmHg vs. 25,4 ± 14,1 mmHg, p=0,028), as compared to the control sub-group (C3)...(AU)

Study of superoxide dismutase's expression in the colon produced by azoxymethane and inositol hexaphosfate's paper, in mice / Estudo da expressão de superóxido dismutase no cólon produzida por azoximetano e o papel do inositol hexafosfato, em ratos

PURPOSE: To investigate the expression of superoxide dismutase (SOD), with use of antioxidant inositol hexaphosfate, in the presence of the carcinogen azoxymethane, in FCA of colon rats. METHODS: Wistar rats (n=48) were distributed in four groups of 12 mice. Divided in control (n=12); with azoxymethane administration AOM (n=12); administration of IP6 (n=12) and with administration of IP6/AOM (n=12). The subcutaneous administration of azoxymethane happened in the week 3 and 4 of the experiment, in dose 20mg/Kg, weekly; and administration of IP6 to 1 percent in water of drinking for 6 weeks in the group 3 and 4. The identification of the expression SOD-1 was accomplished through the quantification imunohistochemistry by the image processing attended by computer in crypts and focus of aberrant crypts in right colon. RESULTS: The group control presented expression of SOD1, on average 16,0 percent; group AOM, 26,7 percent; group IP6, 16,9 percent; group IP6/AOM, 20,9 percent. Variance analysis among the groups, was calculated 0,0078. CONCLUSION: The azoxymethane increase expression SOD1, while inositol hexaphosphate decreases in a significant way the expression of SOD1 promoted by the administration of the carcinogen azoxymethane.(AU)

OBJETIVO: Investigar a expressão de superóxido dismutase, com uso de antioxidante inositol hexafosfato, na presença do carcinógeno azoximetano em FCA de cólon de ratos. MÉTODOS: Quarenta e oito ratos Wistar, distribuídos em 4 grupos, divididos em controle (n=12); com administração de azoximetano AOM (n=12); administração de IP6 (n=12) e com administração de IP6/AOM (n=12). A administração subcutânea de azoximetano aconteceu na semana 3 e 4 do experimento, em dose 20mg/Kg, semanal; e administração de IP6 a 1 por cento em água de beber durante 6 semanas no grupo 3 e 4. A identificação da expressão SOD1 foi realizada através da quantificação imunohistoquimíca pelo processamento de imagem assistida por computador em criptas e focos de cripta aberrante em cólon direito. RESULTADOS: O grupo controle apresentou expressão de SOD1, em média 16,0 por cento; grupo AOM, 26,7 por cento; grupo IP6, 16,9 por cento; grupo IP6/AOM, 20,9 por cento. Análise de variância entre os grupos calculou-se 0,0078. CONCLUSÃO: A expressão de SOD-1 mostrou aumento significativo na presença de azoximetano e quando administrou-se IP6 concomitante houve diminuição na expressão de SOD1 promovido pela administração do carcinógeno azoximetano.(AU)