Glicerina associada à ureia na terminação de bovinos: parâmetros ruminais, digestibilidade e massa microbiana / Glycerin associated with urea in finishing cattle: ruminal fermentation, digestibility and microbial mass

O objetivo desta pesquisa foi verificar a combinação de glicerina com ureia, quanto aos parâmetros ruminais, à digestibilidade e à qualidade da massa microbiana. O trabalho foi conduzido na Unidade Animal de Estudos Digestivos e Metabólicos, pertencente à Faculdade de Ciências Agrárias e Veterinárias-FCAV/Unesp, Câmpus de Jaboticabal. Seis animais da raça Nelore providos de cânulas ruminais foram utilizados para verificar os parâmetros pH, nitrogênio amoniacal, a digestibilidade e a massa microbiana, distribuídos em quadrado latino 6x6. Seis dietas com energia metabolizável e proteína bruta semelhantes foram formuladas, utilizando-se a proporção volumoso:concentrado de 30:70. O volumoso utilizado foi silagem de milho. Os concentrados utilizados foram compostos por milho, casca de soja, farelo de girassol, glicerina e ureia. Os tratamentos foram: C = controle; U = 1% de ureia; G10 = 10% de glicerina; GU10 = 1% de ureia e 10% de glicerina, G20 = 20% de glicerina e GU20 = 20% de glicerina e 1% de ureia. Houve influência das dietas no crescimento dos microrganismos ruminais, no pH e no nitrogênio amoniacal. Dietas com 20% de glicerina na matéria seca da dieta diminuem a população de protozoários ruminais. A inclusão de glicerina diminui a digestibilidade de componentes fibrosos da dieta.(AU)

The aim of this research was to investigate the combination of glycerol with urea as the ruminal parameters, digestibility and quality of microbial mass. The work was conducted at the Animal Unit of Digestive and Metabolic Studies belonging to the Faculty of Agricultural Sciences and Veterinárias-FCAV/Unesp campus Jaboticabal. Six animals fitted with rumen cannulas Nellore were used to verify the parameters pH, ammonia nitrogen, digestibility, and microbial mass distributed in a 6x6 Latin square. Six diets with similar metabolizable energy and crude protein were formulated using the forage:concentrate ratio of 30:70. The roughage used was corn silage. The concentrates used were composed of corn, soybean hulls, sunflower meal, glycerin and urea. The treatments were: C = control, U = 1 % urea, G10 = 10 % glycerin, GU10 = 1 % urea and 10 % glycerol, G20 = 20 % glycerin and GU20 = 20 % glycerin and 1 % urea. Diets with glycerin had lower digestibility of NDF and hemicellulose. There was no influence of diets on the growth of rumen microorganisms, pH and ammonia nitrogen. Diets with 20 % glycerin in the diet dry matter decrease the population of rumen protozoa. The addition of glycerol decreases the digestibility of fibrous components of the diet.(AU)

Glicerina associada à ureia na terminação de bovinos: parâmetros ruminais, digestibilidade e massa microbiana / Glycerin associated with urea in finishing cattle: ruminal fermentation, digestibility and microbial mass

O objetivo desta pesquisa foi verificar a combinação de glicerina com ureia, quanto aos parâmetros ruminais, à digestibilidade e à qualidade da massa microbiana. O trabalho foi conduzido na Unidade Animal de Estudos Digestivos e Metabólicos, pertencente à Faculdade de Ciências Agrárias e Veterinárias-FCAV/Unesp, Câmpus de Jaboticabal. Seis animais da raça Nelore providos de cânulas ruminais foram utilizados para verificar os parâmetros pH, nitrogênio amoniacal, a digestibilidade e a massa microbiana, distribuídos em quadrado latino 6x6. Seis dietas com energia metabolizável e proteína bruta semelhantes foram formuladas, utilizando-se a proporção volumoso:concentrado de 30:70. O volumoso utilizado foi silagem de milho. Os concentrados utilizados foram compostos por milho, casca de soja, farelo de girassol, glicerina e ureia. Os tratamentos foram: C = controle; U = 1% de ureia; G10 = 10% de glicerina; GU10 = 1% de ureia e 10% de glicerina, G20 = 20% de glicerina e GU20 = 20% de glicerina e 1% de ureia. Houve influência das dietas no crescimento dos microrganismos ruminais, no pH e no nitrogênio amoniacal. Dietas com 20% de glicerina na matéria seca da dieta diminuem a população de protozoários ruminais. A inclusão de glicerina diminui a digestibilidade de componentes fibrosos da dieta.(AU)

The aim of this research was to investigate the combination of glycerol with urea as the ruminal parameters, digestibility and quality of microbial mass. The work was conducted at the Animal Unit of Digestive and Metabolic Studies belonging to the Faculty of Agricultural Sciences and Veterinárias-FCAV/Unesp campus Jaboticabal. Six animals fitted with rumen cannulas Nellore were used to verify the parameters pH, ammonia nitrogen, digestibility, and microbial mass distributed in a 6x6 Latin square. Six diets with similar metabolizable energy and crude protein were formulated using the forage:concentrate ratio of 30:70. The roughage used was corn silage. The concentrates used were composed of corn, soybean hulls, sunflower meal, glycerin and urea. The treatments were: C = control, U = 1 % urea, G10 = 10 % glycerin, GU10 = 1 % urea and 10 % glycerol, G20 = 20 % glycerin and GU20 = 20 % glycerin and 1 % urea. Diets with glycerin had lower digestibility of NDF and hemicellulose. There was no influence of diets on the growth of rumen microorganisms, pH and ammonia nitrogen. Diets with 20 % glycerin in the diet dry matter decrease the population of rumen protozoa. The addition of glycerol decreases the digestibility of fibrous components of the diet.(AU)

Energia metabolizável da glicerina bruta para frangos de corte de diferentes idades / Metabolizable energy of crude glycerin for broiler chickens at different ages

Os valores de energia metabolizável aparente (EMA), EMA corrigida pela retenção de nitrogênio(EMAn) e seus respectivos coeficientes de metabolizabilidade (CMA e CMAn) da glicerina bruta foram determinados em frangos de corte utilizando-se o método de coleta total de excretas em diferentes idades. O delineamento utilizado foi inteiramente casualizado com seis tratamentos, seis repetições e dez, cinco e quatro aves por unidade experimental para as fases de 11 a 20 dias, de 21 a 30 dias e de31 a 40 dias, respectivamente. O alimento teste substituiu a ração referência em 0; 3; 6; 9; 12 e 15%,sendo que o período experimental de cada fase teve duração de dez dias, dos quais cinco foram de adaptação e cinco de coleta total de excretas. Os valores de EMA e EMAn para a fase de 11 a 20 dias de idade variaram de 2723 a 3817 e de 2672 a 3667 kcal/kg-1, respectivamente. Na fase de 21 a 30 diasos valores de EMA e EMAn variaram de 3660 a 3982 e de 3535 a 3945 kcal/kg-1, respectivamente.Na fase de 31 a 40 dias os valores de EMA e EMAn variaram de 3029 a 4143 e de 2975 a 4048 kcal/kg-1, respectivamente. Os valores de CMA e CMAn, para a fase de 11 a 20 dias de idade reduziram linearmente, variando de 62,70 a 87,89 e de 61,53 a 84,41%, respectivamente. Na fase de 21 a 30 dias os coeficientes também reduziram linearmente, variando de 84,27 a 91,69 para CMA e de 81,39 a 90,83%para CMAn. Em contraste, durante a fase de 31 a 40 dias, os coeficientes apresentaram efeito linear crescente, valores estes que variaram de 69,76 a 95,39 para CMA e de 68,51 a 93,21% para CMAn. A glicerina bruta pode ser utilizada como fonte energética na alimentação de frangos de corte, pois apresenta valores elevados de energia metabolizável. No entanto, os valores de EMA e EMAn e seus coeficientes de metabolizabilidade da glicerina bruta variam de acordo com os níveis de inclusão na ração referência e da idade das aves.(AU)

The values of apparent metabolizable energy (AME), the AME corrected for nitrogen retention (AMEn)and their respective metabolization coefficients (CME and CMEn) of crude glycerin were determined utilizing the total excreta collection method with broiler chickens at different ages. A randomized complete design was used, with six treatments, six replications and ten, five and four birds per experimental unit in the periods from 11 to 20 days, from 21 to 30 days and from 31 to 40 days of age, respectively. The test food replaced the reference diet in 0; 3; 6; 9; 12 and 15% and the experimental period for each phase lasted ten days, five of which for adaptation and five for total collection. The AME and AMEn values in the phase from 11 to 20 days ranged from 2723 to 3817 and from 2672 to 3667kcal/kg-1, respectively. In the phase from 21 to 30 days the AME and AMEn values ranged from 3660 to3982 and from 3535 to 3945 kcal/kg-1, respectively. In the phase from 31 to 40 days the AME and AMEn values ranged from 3029 to 4143 and from 2975 to 4048 kcal/kg-1, respectively. The values of CME andCMEn linearly decreased from 11 to 20 days of age, ranging from 62.70 to 87.89 and 61.53 to 84.41%,respectively. During the phase from 21 to 30 days the coefficients also linearly decreased, ranging from84.27 to 91.69 for CME and from 81.39 to 90.83% for CMEn. In contrast, during the phase from 31 to40 days, the coefficients linearly increased, values ranging from 69.76 to 95.39 for CME and from 68.51to 93.21% for CMEn. The crude glycerin can be used as an energy source in broiler diets, because it has high levels of metabolizable energy. However, the values of AME and AMEn and the metabolization coefficients of crude glycerin vary according to the levels in the reference diet and age of the birds.(AU)

Biotechnological potential of Clostridium butyricum bacteria

In response to demand from industry for microorganisms with auspicious biotechnological potential, a worldwide interest has developed in bacteria and fungi isolation. Microorganisms of interesting metabolic properties include non-pathogenic bacteria of the genus Clostridium, particularly C. acetobutylicum, C. butyricum and C. pasteurianum. A well-known property of C. butyricum is their ability to produce butyric acid, as well as effectively convert glycerol to 1,3-propanediol (38.2 g/L). A conversion rate of 0.66 mol 1,3-propanediol/mol of glycerol has been obtained. Results of the studies described in the present paper broaden our knowledge of characteristic features of C. butyricum specific isolates in terms of their phylogenetic affiliation, fermentation capacity and antibacterial properties.

Volatile fatty acids influence on the structure of microbial communities producing PHAs

Polyhydroxyalkanoates (PHAs) can be produced by microorganisms and are a biodegradable alternative to fossil-fuel based plastics. Currently, the focus is on reducing production costs by exploring alternative substrates for PHAs production, and on producing copolymers which are less brittle than monomers. Accordingly, this study used a substrate consisting of wastewater from waste-glycerol fermentation, supplemented with different amounts of acetic and propionic acids. These substrates were used to feed mixed microbial communities enriched from activated sludge in a sequencing batch reactor. A reactor supplemented with 2 mL of acetic acid produced 227.8 mg/L of a homopolymer of hydroxybutyrate (3HB); 4 mL of acetic acid produced 279.8 mg/L 3HB; whereas 4 mL of propionic acid produced 673.0 mg/L of a copolymer of 3HB and 3HV (hydroxyvalerate). Ribosomal Intergenic Spacer Analysis (RISA) was used to show the differences between the communities created in the reactors. Thauera species predominated in biomass that produced 3HB; Paracoccus denitrificans in the biomass that produced 3HB-co-3HV. Because P. denitrificans produced the more desirable copolymer, it may be advantageous to promote its growth in PHAs-producing reactors by adding propionate.

Impact of water potential on growth and germination of Fusarium solani soilborne pathogen of peanut

Studies were conducted to determine the effect of osmotic and matric stress on germination and growth of two Fusarium solani strains, the etiological agent responsible of peanut brown root rot. Both strains had similar osmotic and matric potential ranges that allowed growth, being the latter one narrower. F. solani showed the ability to grow down to -14 MPa at 25 °C in non-ionic modified osmotic medium, while under matric stress this was limited to -8.4 MPa at 25 °C. However, both strains were seen to respond differently to decreasing osmotic and matric potentials, during early stages of germination. One strain (RC 338) showed to be more sensitive to matric than osmotic (non ionic) and the other one (RC 386) showed to be more sensitive to osmotic than matric imposed water stress. After 24 h of incubation, both isolates behaved similarly. The minimum water potential for germination was -8.4 MPa on glycerol amended media and -5.6 MPa for NaCl and PEG amended media, respectively. The knowledge of the water potential range which allow mycelia growth and spore germination of F. solani provides an inside to the likely behaviour of this devastating soilborne plant pathogen in nature and has important practical implications.

Glicerina semipurificada vegetal e mista na alimentação de coelhos em crescimento / Vegetal and mixed semipurified glycerin in the feeding of growing rabbits

Dois experimentos foram conduzidos com o objetivo de determinar a energia digestível da glicerina semipurificada vegetal e mista e o desempenho de coelhos em crescimento alimentados com dietas contendo os coprodutos. No ensaio de digestibilidade, foram utilizados 108 coelhos da raça Nova Zelândia Branco, com 45 dias de idade, distribuídos ao acaso em nove tratamentos (0, 4, 8, 12 e 16% de inclusão de glicerina vegetal e mista na dieta), com 12 repetições. Para o ensaio de desempenho, utilizaram-se 180 coelhos da raça Nova Zelândia Branco, dos 32 aos 70 dias de idade, distribuídos ao acaso em arranjo fatorial 2 x 4 (dois tipos de glicerina e quatro porcentagens de inclusão: 3, 6, 9 e 12%) mais uma dieta referência, com 10 repetições e dois animais por unidade experimental. Glicerinas vegetal e mista apresentaram energia digestível de 4.048 e 3.697kcal/kg MS, respectivamente. Entre 32 e 50 dias de idade dos coelhos, a dieta com 12% de glicerina mista prejudicou (P<0,05) o ganho de peso (32,14 g/dia), a conversão alimentar (3,57) e o custo por quilo de ganho de peso (R$ 2,08) em relação à dieta referência (39,42g/dia, 2,87 e R$1,69, respectivamente). Dos 32 aos 70 dias, o ganho de peso (30,11g/dia) e a conversão alimentar (3,99) dos animais que receberam 12% de glicerina mista na dieta foram piores (P<0,05) em relação aos da dieta referência (34,00g/dia e 3,65, respectivamente). Para as características de desempenho, dos 32 aos 70 dias, não foram verificadas diferenças (P>0,05) entre a inclusão de glicerina vegetal na dieta e a dieta referência, porém a maior viabilidade econômica (P<0,05) foi com 12% de inclusão (R$1,89 x R$2,15, respectivamente). A glicerina vegetal pode ser incluída em até 12% da dieta e a mista, em até 9%, diminuindo o custo de produção sem afetar o desempenho animal.(AU)

Two trials were conducted with the goal of determining the digestible energy of vegetal and mixed semi purified glycerin and the performance of growing rabbits fed with diets containing the byproducts. In the digestibility assay 108 New Zealand White rabbits, 45 days old, were used, assigned in a completely randomized design into nine treatments (0, 4, 8, 12 and 16% of glycerins inclusion), with 12 replications. The treatment with 0% of glycerin inclusion was considered the reference diet. In the performance assay, 180 New Zealand White rabbits, 32 to 70 days of age, were assigned to a completely randomized design into a 2 × 4 factorial arrangement (two types of glycerin and four percentages of inclusion: 3, 6, 9 and 12%) plus a reference diet and ten replications with two animals per experimental unit. Vegetal and mixed glycerin showed a digestible energy of 4,048 and 3,697 kcal/kg of dry matter, respectively. From 32 to 50 days of age, the diet with 12% of mixed glycerin affected (P<0.05) weight gain (32.14g/day), feed conversion (3.57) and cost per kilo of weight gain (R$2.08) in relation to the reference diet (39.42g/day, 2.87 and R$ 1.69, respectively). From 32 to 70 days, weight gain (30.11g/day) and feed conversion (3.99) of the animals fed with the diet containing 12% of mixed glycerin were worst (P<0.05) in relation to the reference diet (34.00 g/day and 3.65, respectively). Regarding characteristics of animal performance, from 32 to 70 days, no differences (P>0.05) were observed between the inclusion of vegetal glycerin in the diet and the reference diet, but better economic viability was verified (P<0.05) with the diet containing 12% of vegetal glycerin (R$1.89 x R$2.15, respectively). Vegetal glycerin can be included up to 12% in the diet and mixed up to 9%, reducing production cost without affecting animal performance.(AU)

Energia metabolizável da glicerina bruta para frangos de corte de diferentes idades / Metabolizable energy of crude glycerin for broiler chickens at different ages

Os valores de energia metabolizável aparente (EMA), EMA corrigida pela retenção de nitrogênio(EMAn) e seus respectivos coeficientes de metabolizabilidade (CMA e CMAn) da glicerina bruta foram determinados em frangos de corte utilizando-se o método de coleta total de excretas em diferentes idades. O delineamento utilizado foi inteiramente casualizado com seis tratamentos, seis repetições e dez, cinco e quatro aves por unidade experimental para as fases de 11 a 20 dias, de 21 a 30 dias e de31 a 40 dias, respectivamente. O alimento teste substituiu a ração referência em 0; 3; 6; 9; 12 e 15%,sendo que o período experimental de cada fase teve duração de dez dias, dos quais cinco foram de adaptação e cinco de coleta total de excretas. Os valores de EMA e EMAn para a fase de 11 a 20 dias de idade variaram de 2723 a 3817 e de 2672 a 3667 kcal/kg-1, respectivamente. Na fase de 21 a 30 diasos valores de EMA e EMAn variaram de 3660 a 3982 e de 3535 a 3945 kcal/kg-1, respectivamente.Na fase de 31 a 40 dias os valores de EMA e EMAn variaram de 3029 a 4143 e de 2975 a 4048 kcal/kg-1, respectivamente. Os valores de CMA e CMAn, para a fase de 11 a 20 dias de idade reduziram linearmente, variando de 62,70 a 87,89 e de 61,53 a 84,41%, respectivamente. Na fase de 21 a 30 dias os coeficientes também reduziram linearmente, variando de 84,27 a 91,69 para CMA e de 81,39 a 90,83%para CMAn. Em contraste, durante a fase de 31 a 40 dias, os coeficientes apresentaram efeito linear crescente, valores estes que variaram de 69,76 a 95,39 para CMA e de 68,51 a 93,21% para CMAn. A glicerina bruta pode ser utilizada como fonte energética na alimentação de frangos de corte, pois apresenta valores elevados de energia metabolizável. No entanto, os valores de EMA e EMAn e seus coeficientes de metabolizabilidade da glicerina bruta variam de acordo com os níveis de inclusão na ração referência e da idade das aves.

The values of apparent metabolizable energy (AME), the AME corrected for nitrogen retention (AMEn)and their respective metabolization coefficients (CME and CMEn) of crude glycerin were determined utilizing the total excreta collection method with broiler chickens at different ages. A randomized complete design was used, with six treatments, six replications and ten, five and four birds per experimental unit in the periods from 11 to 20 days, from 21 to 30 days and from 31 to 40 days of age, respectively. The test food replaced the reference diet in 0; 3; 6; 9; 12 and 15% and the experimental period for each phase lasted ten days, five of which for adaptation and five for total collection. The AME and AMEn values in the phase from 11 to 20 days ranged from 2723 to 3817 and from 2672 to 3667kcal/kg-1, respectively. In the phase from 21 to 30 days the AME and AMEn values ranged from 3660 to3982 and from 3535 to 3945 kcal/kg-1, respectively. In the phase from 31 to 40 days the AME and AMEn values ranged from 3029 to 4143 and from 2975 to 4048 kcal/kg-1, respectively. The values of CME andCMEn linearly decreased from 11 to 20 days of age, ranging from 62.70 to 87.89 and 61.53 to 84.41%,respectively. During the phase from 21 to 30 days the coefficients also linearly decreased, ranging from84.27 to 91.69 for CME and from 81.39 to 90.83% for CMEn. In contrast, during the phase from 31 to40 days, the coefficients linearly increased, values ranging from 69.76 to 95.39 for CME and from 68.51to 93.21% for CMEn. The crude glycerin can be used as an energy source in broiler diets, because it has high levels of metabolizable energy. However, the values of AME and AMEn and the metabolization coefficients of crude glycerin vary according to the levels in the reference diet and age of the birds.