Influence of oophorectomy on glycemia and lipidogram

Purpose: To verify the influence of oophorectomy on lipidogram and glycemia. Methods: Fifty six female rats were divided into the following 7 groups (n = 8): group 1 - sham group, group 2 - oophorectomy 30 days, group 3 - oophorectomy 35 days, group 4 - oophorectomy 40 days, group 5 - oophorectomy 45 days, group 6 - oophorectomy 70 days, group 7 - oophorectomy 55 days. Animals were following by number of days according the group. Was evaluated the serum levels of glucose and lipid profile. Results: The oophorectomized rats presented higher glycemia. Groups 3, 4, 6 and 7 had a higher glycemia and LDL levels (except for group 6) and groups 6 and 7 had lowest levels of HDL. Group 7 had highest level of VLDL than oophorectomy groups. There was no difference in triglycerides levels. Conclusion: Oophorectomy was related to dyslipidemia and insulin resistance, mainly after 50th days.(AU)

Effect of ovariectomy in bone structure of mandibular condyle

Purpose: To evaluate the bone structure of the mandibular condyle through an animal model, after ovariectomy. Methods: Thirty-six female Wistar rats were divided into 2 groups. The OVX group was submitted to bilateral ovariectomy, the SHAM group underwent the same surgical treatment, but without removal of the ovaries. After 90, 105 and 135 days after surgery, six animals from each group were submitted to euthanasia and the part containing the condyle was removed. Results: The microscopic analysis shows an increase in marrow spaces over time in the OVX group. The morphometric study shows reduction in the amount of bone tissue in the OVX group 135 days period in comparison with for the initial period (90 days) (p 0.05, ANOVA, Tukey). Conclusion: The estrogen deficiency also affects the bone structure of the condyle.(AU)

Complicações associadas à ovariosalpingohisterectomia eletiva realizada com abraçadeira de náilon como método de hemostasia / Complications associated with ovariohysterectomy using nylon tie-rap as an hemostatic method

Background: Routine neutering of bitches is typically carried out by ovariohysterectomy (OVH) in Veterinary Medicine, seen by many as a simple procedure. During the conventional approach the points of attachment of the uterus and ovaries are double ligated with absorbable or non-absorbable sutures or even with titanium clamps. It has been described the use of non-surgical polyamide tie-raps as a hemostatic closure method by some authors but there are few papers describing the consequenses of such use. The aim of this study is to report four cases of surgical complications associated with nylon tie-raps use on OVHs on bitches referred to the Veterinary Teaching Hospitals of the Federal University of Rio Grande do Sul (HCV-UFRGS) and Passo Fundo University (HV-UPF), both at the State of Rio Grande do Sul. Cases: 1- An eleven-month-old bitch was admitted at the HV-UPF nine days after OVH performed elsewhere, with omentum evisceration due to suture dehiscence. During emergency surgery both ovaries were found surrounded by nylon tie-raps and fibrous tissue adherence. Peritonitis signs were recognizable. Bilateral ovariectomy with nylon 2-0 was performed and the remaining uterus was removed. 2- A five-years-old bitch was referred to the HV-UPF ten days after OVH performed elsewhere, with apathy and suture dehiscence. Free liquid was identifi ed around the bladder and kidneys using the ultrasound exam. Intense hemorrhage from uterine vessels was found during celiotomy. A nylon tie-rap was found loose on the abdominal cavity and another couple were found at both ovaries surrounded by fibrous tissue adherence. Bilateral ovariectomy with nylon 2-0 was performed and a blood transfusion was necessary. 3- A three-years-old bitch was admitted at the HCV-UFRGS six days after OVH performed at another institution, presenting suture dehiscence and evisceration. During emergency surgery, bladder, intestine and musculature necrosis due to the contact with sharp edged nylon tie-raps (prior placed as hemostatic devices), intense fibrous tissue adherence and peritonitis were identified. 4- A two-years-old bitch was referred to the HCV-UFRGS one year after OVH performed elsewhere, with a four-month persistent sinus tract on the right flank unsuccessfully treated by another veterinarian. Abdominal communication was suspected and therefore the patient was submitted to exploratory celiotomy. During surgery intense fibrous tissue adherence were found on both mesovarium. Stump granuloma and a sinus tract were also identified in the right side. Sharped nylon tie-raps were found among the fibrous tissue and were then replaced by non-absorbable suture. Discussion: OVH complications such as suture dehiscence, evisceration, peritonitis and loosen tie-raps, all seen on cases 1, 2 and 3 are usual when there is trans-operatory contamination and can probably be associated with technique failure, as described by other authors. Self-trauma must be avoided throughout the use of Elizabethan collar, but this recommendation was not followed by the patients' owners on any of the cases even though it had been prescribed by the veterinarian. Fibrous tissue and granulomas were found on all cases due to the tie-raps, what brings the question about its real effects to the animal organism. All the patients had satisfiable recovery after the emergency surgeries. There is urgent need of scientific investigations about the grave consequences of the inadequate use of poliamide tie-raps before assigning it as a hemostatic material.

Manifestação incomum de paralisia de membros pélvicos em felino com hidronefrose decorrente de ovarioectomia ­ relato de caso / Limbs paralyze in a cat with hydronephrosis following ovariectomy - case report

Neste trabalho, descreve-se a ocorrência de hidronefrose iatrogênica em rim esquerdo, comprimindo artéria aorta abdominal em gato persa e provocando paralisia de membros pélvicos. O animal apresentava discreta desidratação, mucosas hipocoradas, extremidades frias e cianóticas, ausência bilateral de pulso da artéria femoral, bem como insensibilidade superficial e profunda, e presença de massa de consistência firme com seis centímetros de diâmetro, à palpação abdominal. Realizada celiotomia exploratória, verificou-se rim esquerdo aumentado comprimindo artéria aorta abdominal e a dilatação cranial do ureter esquerdo fixado ao corno uterino por meio de ligadura com fio não absorvível. Após nefrectomia, observou-se a restauração da circulação local. O animal foi a óbito, após oito horas.

This study describes the occurrence of iatrogenic hydronephrosis in left kidney with compression of the abdominal aorta in persian cat and paralysis of hind limbs. The animal had slight dehydration, pale mucous membranes, cold extremities and cyanotic, absence of bilateral femoral artery pulse, and absence of superficial and deep pain, and presence of a mass of firm consistency with six centimeters in diameter, on abdominal palpation. Performed exploratory celiotomy, there was increased left kidney compressing the abdominal aorta and dilation of the cranial left ureter attached to the uterine horn by means of ligation with nonabsorbable. After nephrectomy, it was observed the restoration of local circulation. The animal died after eight hours.

The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats / Efeito da ooforectomia no tumor de Walker 256 inoculado em vagina e colo de útero de ratos fêmeas

PURPOSE: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. METHODS: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. RESULTS: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. CONCLUSION: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass.(AU)

OBJETIVO: Verificar o efeito da ooforectomia à inoculação do tumor de Walker 256 em vagina e colo de útero de ratas. MÉTODOS: Foram utilizadas 10 ratas Wistar, fêmeas, virgens, adultas, distribuídas em dois grupos de estudo com 05 animais cada: grupo tumor (GT): ratas inoculadas com tumor de Walker 256, e grupo Ooforectomia (GO): ratas ooforectomizadas e inoculadas com tumor de Walker 256. No dia anterior à inoculação vaginal do tumor, foram inoculados 0,3ml de ácido acético na vagina das ratas de ambos os grupos; no dia seguinte, foi realizada a escarificação da parede vaginal com uma escova de endocérvice e inoculado tumor de Walker 256. Após 12 dias, foi removido o tumor em bloco com vagina e cornos uterinos para análise macro e microscópica. Os dados foram submetidos à análise estatística. RESULTADOS: Não houve diferença estatística entre os dois grupos; no entanto, notou-se que o comportamento de crescimento do tumor no grupo GO apresentou maior invasividade em extensão, comprometendo cornos uterinos. CONCLUSÃO: Observou-se que o grupo GO apresentou comportamento macroscópico diferente ao grupo GT, no entanto, em termos totais de massa tumoral ambos apresentam desenvolvimento similar.(AU)