Dermatose actínica em cão: relato de caso / Actinic dermatosis in dogs: a cse report

O trabalho tem o objetivo de relatar um caso de Dermatose Actínica em cadela adulta, 8 anos apresentando pele eritematosa. Na avaliação clínica a cadela apresentou os parâmetros normais da espécie, porém devido às lesões observadas foram solicitados exames citológico e parasitológico de pele, que demonstraram a presença de bactérias (cocos), neutrófilos degenerados e fungos dermatófitos além de demodicidose. O tratamento consistiu do uso de inúmeras drogas como itraconazol, amoxicilina com clavulonato de potássio, predinisolona, Sarolaner e imunomoduladores, além de tratamento tópico com cetoconazol shampoo e creme. Geralmente, as piodermites profundas que atingem o folículo de cães e formam feridas ulceradas, causam uma inflamação crônica na pele, por conta de raios-ultravioletas do sol, ou predisposição familiar ou hipersensibilidade microbiana, ou por outras doenças e, normalmente, são reincidentes e não permitem a cura. Entretanto, nesse caso específico, o tratamento permitiu a melhora do animal (~80%).(AU)

The aim of this paper is to report a case of Actinic Dermatosis in an 8-year-old female adult female with erythematous skin. In the clinical evaluation, the animal presented normal parameters for the species, but, due to the lesions observed, cytological and parasitological skin tests were requested, which showed the presence of bacteria (cocci), degenerated neutrophils, and dermatophyte fungi, and demodicidosis. Treatment consisted of the use of numerous drugs such as itraconazole, amoxicillin with potassium clavulonate, predinisolone, Sarolaner, and immunomodulators; in addition to topical treatment with ketoconazole shampoo and cream. Generally, deep pyoderma that reach the follicle of dogs and form ulcerated wounds, cause a chronic inflammation of the skin, due to the sun's ultraviolet rays, or familial predisposition or microbial hypersensitivity, or due to other diseases and, usually, they are recurrent and do not allow healing. However, in this specific case, the treatment allowed the animal to improve (~80%).(AU)

Histopathological and parasitological analysis of skin tissues biopsies from two distinct anatomical areas of the ears of dogs naturally infected with Leishmania (Leishmania) chagasi

Canine visceral leishmaniasis is an endemic disease in Latin America caused by Leishmania (Leishmania) chagasi and transmitted to man and animals by infected blood-sucking sandflies) of the genus Lutzomyia. Dogs are considered to be the primary domestic reservoir of disease because they present an intense cutaneous parasitism. The aim of this study was to evaluate the intensity of the inflammatory process and to compare it to the parasite load of tissue from two different sites of the ear skin of dogs naturally infected with Leishmania chagasi. We think that exist a specific anatomical region that exhibits a relatively higher rate of parasitism. For diagnostic analysis, serological tests were carried out using the indirect fluorescence antibody test (IFAT) and the enzyme-linked immunosorbent assay (ELISA). Twelve animals naturally infected with Leishmania chagasi were euthanatized with a lethal dose of Sodium Thiopental™ and T61™ During the necropsy, fragments of the extremity and middle anatomical regions of the ear were collected. All tissues were fixed in a 10% formalin solution and then paraffin-embedded for histopathological (HE) and immunohistochemical analysis. The streptavidin-peroxidase immunohistochemistry method was used to detect tissue amastigotes using optical microscopy. Our results indicated a chronic inflammatory reaction, ranging from discrete to an intense magnitude. The inflammatory process was more frequently observed in the extremity of the ear than in the middle portion of the ear (p<0.05). The presence of parasites in the ear extremity was higher than in other evaluated regions. A positive correlation between the tissue inflammation, parasitism, and serological data was confirmed at both ear positions (p<0.05). Skin biopsies are an important tool for CVL diagnosis and the ear extremity represents an appropriated area to perform the assays.