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1.
Arq. ciênc. vet. zool. UNIPAR ; 19(3): 131-135, jul.-set. 2016. graf, tab
Artigo em Português | VETINDEX | ID: vti-691074

Resumo

O objetivo do presente trabalho foi avaliar métodos de controle e tratamento de bacterioses na larvicultura de Litopenaeus vannamei, avaliando o uso contínuo de probiótico e o uso pontual de enrofloxacino 15mg.L-1 e propionato de sódio 0,5 mM.L-1 nos momentos de metamorfose sobre os, parâmetros zootécnicos e microbiológicos (larvas e da água). Foram utilizadas 16 unidades de 60L, povoadas na densidade de 325 náuplios.5L-1, divididos em três tratamentos: enrofloxacino, probiótico, propionato de sódio e controle. O enrofloxacino e o propionato foram ministrados em protozoea 3, misis 3 e pós-larva 4 e o probiótico foi ministrado na ração ao longo de todo o experimento. O probiótico aumentou as contagens de bactérias ácido-láticas em relação aos demais tratamentos na água de cultivo (p=0,00001) e em relação ao enrofloxacino e o proprionato nas larvas (p=0,0048). A água do tratamento com probiótico apresentou menor contagem de Vibrio spp. que o enrofloxacino (p=0,0011) e as larvas tratadas com probiótico apresentaram menor contagem de Vibrio spp. que o propionato (p=0,0158). Contudo, não foi observada diferença nos índices zootécnicos avaliados. Assim, os aditivos na dose utilizada não alteram parâmetros zootécnicos da larvicultura do camarão L. vannamei.(AU)


The purpose of this study was to assess control and treatment methods for bacterial diseases in Litopenaeus vannamei, evaluating the continuous use of probiotics and occasional use of 15mg-L-1 enrofloxacin and 0.5-mM.L-1 sodium propionate at the morphological change moments on the performance and microbiological parameters of larvae and water. A total of 16 60-L units were used, stocked with 325 nauplii/5L-1, divided into three treatments and one control. Enrofloxacin and propionate were administered into protozoea 3, misis 3 and 4, and post-larvae 4, while the probiotic was administered in the feed throughout the experiment. The probiotic increased the counts of lactic acid bacteria in relation to the other treatments in the culture water (p = 0.00001) and in relation to enrofloxacin and propionate in larvae (p = 0.0048). The treatment water with probiotic had lower counts of Vibrio ssp. than enrofloxacin (p = 0.0011) and larvae treated with probiotic showed lower counts of Vibrio ssp. that propionate (p = 0.0158). However, no difference was observed in the performance indexes assessed. Thus, it can be concluded that additives in the assessed doses did not influence the performance parameters of L. vannamei.(AU)


El objetivo del presente trabajo fue evaluar métodos de control y tratamiento de enfermedades bacterianas en larvicultura de Litopenaeus vannamei, evaluando el uso continuo de probiótico y el uso puntual de 15 mg.L-1 de enrofloxacino y 0,5 mM.L-1 de propionato de sodio en momentos de metamorfosis sobre los parámetros zootécnicos y microbiológicos (larvas y del agua). Se han utilizado 16 unidades de 60L, pobladas en la densidad de 325 nauplios.5L-1, divididos en tres tratamientos: enrofloxacino, probiótico, propionato de sodio y control. El enrofloxacino y el propionato fueron suministrados en protozoea 3, misis 3 y postlarva 4 y el probiótico suministrado en alimento durante el transcurso del experimento. El probiótico aumentó el contaje de bacterias ácido-lácticas en correlación a los demás tratamientos en agua de cultivo (p=0,0001) y las larvas en relación al enrofloxacino y el propionato en las larvas (p=0,0048). El agua del tratamiento con probiótico presentó menor contaje de Vibrio spp. que el enrofloxacino (p=0,0011) y las larvas tratadas con probiótico presentaron menor contaje de Vibrio spp. que el propionato (p=0,0158). Sin embargo, no se ha observado diferencia en los índices zootécnicos de larvicultura de camarón L. vannamei.(AU)


Assuntos
Animais , Penaeidae/virologia , Penaeidae , Vibrioses/prevenção & controle , Prevenção de Doenças , Propionatos
2.
Arq. ciênc. vet. zool. UNIPAR ; 19(3): 131-135, jul.-set. 2016. graf, tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-833148

Resumo

O objetivo do presente trabalho foi avaliar métodos de controle e tratamento de bacterioses na larvicultura de Litopenaeus vannamei, avaliando o uso contínuo de probiótico e o uso pontual de enrofloxacino 15mg.L-1 e propionato de sódio 0,5 mM.L-1 nos momentos de metamorfose sobre os, parâmetros zootécnicos e microbiológicos (larvas e da água). Foram utilizadas 16 unidades de 60L, povoadas na densidade de 325 náuplios.5L-1, divididos em três tratamentos: enrofloxacino, probiótico, propionato de sódio e controle. O enrofloxacino e o propionato foram ministrados em protozoea 3, misis 3 e pós-larva 4 e o probiótico foi ministrado na ração ao longo de todo o experimento. O probiótico aumentou as contagens de bactérias ácido-láticas em relação aos demais tratamentos na água de cultivo (p=0,00001) e em relação ao enrofloxacino e o proprionato nas larvas (p=0,0048). A água do tratamento com probiótico apresentou menor contagem de Vibrio spp. que o enrofloxacino (p=0,0011) e as larvas tratadas com probiótico apresentaram menor contagem de Vibrio spp. que o propionato (p=0,0158). Contudo, não foi observada diferença nos índices zootécnicos avaliados. Assim, os aditivos na dose utilizada não alteram parâmetros zootécnicos da larvicultura do camarão L. vannamei.


The purpose of this study was to assess control and treatment methods for bacterial diseases in Litopenaeus vannamei, evaluating the continuous use of probiotics and occasional use of 15mg-L-1 enrofloxacin and 0.5-mM.L-1 sodium propionate at the morphological change moments on the performance and microbiological parameters of larvae and water. A total of 16 60-L units were used, stocked with 325 nauplii/5L-1, divided into three treatments and one control. Enrofloxacin and propionate were administered into protozoea 3, misis 3 and 4, and post-larvae 4, while the probiotic was administered in the feed throughout the experiment. The probiotic increased the counts of lactic acid bacteria in relation to the other treatments in the culture water (p = 0.00001) and in relation to enrofloxacin and propionate in larvae (p = 0.0048). The treatment water with probiotic had lower counts of Vibrio ssp. than enrofloxacin (p = 0.0011) and larvae treated with probiotic showed lower counts of Vibrio ssp. that propionate (p = 0.0158). However, no difference was observed in the performance indexes assessed. Thus, it can be concluded that additives in the assessed doses did not influence the performance parameters of L. vannamei.


El objetivo del presente trabajo fue evaluar métodos de control y tratamiento de enfermedades bacterianas en larvicultura de Litopenaeus vannamei, evaluando el uso continuo de probiótico y el uso puntual de 15 mg.L-1 de enrofloxacino y 0,5 mM.L-1 de propionato de sodio en momentos de metamorfosis sobre los parámetros zootécnicos y microbiológicos (larvas y del agua). Se han utilizado 16 unidades de 60L, pobladas en la densidad de 325 nauplios.5L-1, divididos en tres tratamientos: enrofloxacino, probiótico, propionato de sodio y control. El enrofloxacino y el propionato fueron suministrados en protozoea 3, misis 3 y postlarva 4 y el probiótico suministrado en alimento durante el transcurso del experimento. El probiótico aumentó el contaje de bacterias ácido-lácticas en correlación a los demás tratamientos en agua de cultivo (p=0,0001) y las larvas en relación al enrofloxacino y el propionato en las larvas (p=0,0048). El agua del tratamiento con probiótico presentó menor contaje de Vibrio spp. que el enrofloxacino (p=0,0011) y las larvas tratadas con probiótico presentaron menor contaje de Vibrio spp. que el propionato (p=0,0158). Sin embargo, no se ha observado diferencia en los índices zootécnicos de larvicultura de camarón L. vannamei.


Assuntos
Animais , Penaeidae/microbiologia , Penaeidae/virologia , Vibrioses/prevenção & controle , Prevenção de Doenças , Propionatos
3.
Braz. j. vet. pathol ; 8(2): 51-57, Jul. 2015. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1469926

Resumo

In Ecuador, the development and sustainability of the cultured white shrimp, Penaeus vannamei, has been threatened by the occurrence of several viral pathogens, Infectious hypodermal and haematopoietic necrosis virus (IHHNV) and White spot syndrome virus (WSSV) mainly. The aim of the present study was to evaluate the exposition of P. vannamei juveniles to IHHNV and formalin-inactivated viruses (inactWSSV or inactIHHNV) to induce a protective response in P. vannamei juveniles against WSSV infection. P. vannamei were challenged to WSSV by intramuscular injection. Shrimp mortalities appeared at day 1 post-injection (p.i.) in positive control and inactIHHNV treatment, while IHHNV and inactWSSV treatments presented onset of mortalities at day 2 p.i. Positive control and inactIHHNV treatment presented 100% mortality at day 4 p.i., while IHHNV and inactWSSV treatments reached similar mortality at day 6 p.i. Statistical analysis revealed that WSSV-induced mortalities in juvenile P. vannamei of IHHNV and inactWSSV treatments had a significant delay (P 0.05) compared to both the inactIHHNV-treatment and positive control. Our results showed thatpreliminary exposure to IHHNV or to formalin-inactivated WSSV can induce delayed mortality in Penaeus vannameifollowing challenge with WSSV via intramuscular injection. In case of IHHNV infection, viral interference could be thebiological phenomenon involved, mediated by competition between IHHNV and WSSV. Regarding to WSSV inactivatedby formalin, a “vaccination” response would be responsible for the delay, evidencing a possible specific antiviral immuneresponse from the host.


Assuntos
Animais , Penaeidae/virologia , Vírus da Necrose Hematopoética Infecciosa , Vírus da Síndrome da Mancha Branca 1 , Formação de Anticorpos
4.
Braz. J. Vet. Pathol. ; 8(2): 51-57, Jul. 2015. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-22936

Resumo

In Ecuador, the development and sustainability of the cultured white shrimp, Penaeus vannamei, has been threatened by the occurrence of several viral pathogens, Infectious hypodermal and haematopoietic necrosis virus (IHHNV) and White spot syndrome virus (WSSV) mainly. The aim of the present study was to evaluate the exposition of P. vannamei juveniles to IHHNV and formalin-inactivated viruses (inactWSSV or inactIHHNV) to induce a protective response in P. vannamei juveniles against WSSV infection. P. vannamei were challenged to WSSV by intramuscular injection. Shrimp mortalities appeared at day 1 post-injection (p.i.) in positive control and inactIHHNV treatment, while IHHNV and inactWSSV treatments presented onset of mortalities at day 2 p.i. Positive control and inactIHHNV treatment presented 100% mortality at day 4 p.i., while IHHNV and inactWSSV treatments reached similar mortality at day 6 p.i. Statistical analysis revealed that WSSV-induced mortalities in juvenile P. vannamei of IHHNV and inactWSSV treatments had a significant delay (P 0.05) compared to both the inactIHHNV-treatment and positive control. Our results showed thatpreliminary exposure to IHHNV or to formalin-inactivated WSSV can induce delayed mortality in Penaeus vannameifollowing challenge with WSSV via intramuscular injection. In case of IHHNV infection, viral interference could be thebiological phenomenon involved, mediated by competition between IHHNV and WSSV. Regarding to WSSV inactivatedby formalin, a “vaccination” response would be responsible for the delay, evidencing a possible specific antiviral immuneresponse from the host.(AU)


Assuntos
Animais , Penaeidae/virologia , Vírus da Necrose Hematopoética Infecciosa , Vírus da Síndrome da Mancha Branca 1 , Formação de Anticorpos
5.
Braz. j. microbiol ; 46(3): l7235-723, July-Sept. 2015. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1469613

Resumo

Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems..


Assuntos
Animais , Adenoviridae/classificação , Adenoviridae/isolamento & purificação , Fezes/virologia , Penaeidae/virologia , Poluição da Água , Brasil , Ecossistema , Esgotos/virologia , Geografia , Reação em Cadeia da Polimerase em Tempo Real
6.
Braz. J. Microbiol. ; 46(3): l7235, July-Sept. 2015. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-5172

Resumo

Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems..(AU)


Assuntos
Animais , Adenoviridae/classificação , Adenoviridae/isolamento & purificação , Fezes/virologia , Penaeidae/virologia , Poluição da Água , Brasil , Ecossistema , Geografia , Reação em Cadeia da Polimerase em Tempo Real , Esgotos/virologia
7.
Braz. j. vet. pathol ; 4(3): 214-218, nov. 2011. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1398641

Resumo

White Spot Syndrome Virus (WSSV) was isolated from diseased shrimps presenting with clinical signs of WSSV infection. The seed virus was identified as WSSV by PCR, and used to inoculate to specific pathogen free (SPF) P. vannamei bloodstocks. WSSV was purified as described by Huang from infected gills from inoculated animals that were homogenized in a blender and partially purified by differential centrifugation. The final purification was carried out using density gradient in 10-40% NaBr and purified virus used for morphological analysis using transmission electron microscopy. A negative staining method using 2% PTA was used for purified virus, and electron staining with lead citrate and uranylacetate was used for ultra thin sections of infected tissues. This analysis determined that i) the spikes of intact virions could be clearly identified on the virion surface and on a partially broken envelopes, ii) the nucleocapsid structures were similar to those previously reported, even though the so called "ring" structure described previously was different and, iii) negative staining of purified WSSV fractions identified nucleosome like structures.(AU)


Assuntos
Animais , Penaeidae/virologia , Vírus da Síndrome da Mancha Branca 1/isolamento & purificação , Reação em Cadeia da Polimerase/métodos
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