Detecção sorológica e microbiológica de Salmonella spp. em emas (Rhea americana) / Serological and microbiological detection of Salmonella spp. in rheas (Rhea americana)

The fast agglutination screening test, using S. Typhimurium as the antigen, was compared with the standard bacterial method to identify rheas (Rhea americana) contaminated with Salmonella spp.at slaughter.Seventy birds were serologically tested for Salmonella enterica Pullorum using a commercial antigen.Of these, 66 were also submitted to a macroagglutination test, using a strain of Salmonella Typhimurium isolated from rheas.All birds did not react with the commercial S. Pullorum antigen, but 37 were positive for the FAST-ST.The isolation of Salmonella spp. was verified in 66 (94.2%) birds. 85.7% were found in liver samples, 60% in feces and 42.3% in cloacal swabs.A total of 16.6% were identified as being S. enterica enterica rugosa, 35.9% as S. Typhimurium, 46.5% as S.Newport and 0.9% as S. Anatum. An insignificant concordance between the results of bacterial isolation and the serological response was observed (k=0.016).The detection of Salmonella spp. by bacteriological and serological methods in samples from rheas must be deemed important, because birds without a clinical manifestation can be significant sources of salmonellas in food infections.

Diagnóstico microbiológico e histopatológico de mortalidade em avestruzes (Struthio camelus) / Microbiological and histological diagnosis in mortality of ostrich (Struthio camelus)

Several young ostrich, including nestlings, with lassitude and inappetence followed by death or victim of sudden death were immediately brought to diagnosis at an Animal Health Laboratory. At necropsy, animals presented hemorrhage and altered content of the vitelline sac, and necrotic foci in the small intestine; one animal showed necrotic pleuropneumonia with psammomatosus bodies in the lung parenchyma. The cultures from different samples revealed Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Enterobacter aglomerans, and Pseudomonas mendocina. It was suggested one case of septicemia in an animal with exclusive growth of K. pneumoniae isolated from samples of small intestine, lung, and liver.(AU)