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1.
Braz. j. biol ; 83: e240118, 2023. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1278559

Resumo

Abstract For many centuries human populations have been suffering and trying to fight with disease-bearing mosquitoes. Emerging and reemerging diseases such as Dengue, Zika, and Chikungunya affect billions of people around the world and recently has been appealing to control with chemical pesticides. Malathion (MT) is one of the main pesticides used against mosquitoes, the vectors of these diseases. This study aimed to assess cytotoxicity and mutagenicity of the malathion for the bioindicator Allium cepa L. using a multivariate and integrative approach. Moreover, an appendix table was compiled with all available literature of insecticides assessed by the Allium cepa system to support our discussion. Exposures during 48h to 0.5 mg mL-1 and 1.0 mg mL-1 MT were compared to the negative control (distilled water) and positive control (MMS solution at 10 mg L-1). The presence of chromosomal aberrations, micronuclei frequency, and mitotic index abnormalities was evaluated. Anaphase bridges were the alterations with higher incidence and presented a significantly elevated rate in the concentration of 0.5 mg mL-1, including when compared to the positive control. The integrative discriminant analysis summarizes that MT in assessed concentrations presented effects like the positive control, corroborating its potential of toxicity to DNA. Therefore, it is concluded that MT in its pure composition and in realistic concentrations used, has genotoxic potential in the biological assessment of A. cepa cells. The multivariate integrative analysis was fundamental to show a whole response of all data, providing a global view of the effect of MT on DNA.


Resumo Por muitos séculos, as populações humanas sofrem e tentam combater os mosquitos transmissores de doenças. Doenças emergentes e reemergentes como Dengue, Zika e Chikungunya afetam bilhões de pessoas em todo o mundo e, recentemente, vem apelando ao controle com pesticidas químicos. O Malation (MT) é um dos principais pesticidas usados ​​contra mosquitos, vetores dessas doenças. O objetivo deste estudo foi avaliar a citotoxicidade e a mutagenicidade do MT para o bioindicador Allium cepa L. usando uma abordagem multivariada e integrativa. Além disso, uma tabela suplementar foi compilada com toda a literatura disponível de inseticidas avaliada pelo sistema Allium cepa para apoiar nossa discussão. Exposições ao MT durante 48h a 0,5 mg mL-1 e 1,0 mg mL-1 foram comparadas a um controle negativo (água destilada) e um controle positivo (10 mg L-1 de MMS). Foram avaliadas a presença de aberrações cromossômicas, frequência de micronúcleos e anormalidades no índice mitótico. As pontes anafásicas foram as alterações com maior incidência e apresentaram uma taxa significativamente elevada na concentração de 0,5 mg mL-1, inclusive quando comparadas ao controle positivo. A análise discriminante integrativa resume que o MT nas concentrações avaliadas apresentou efeitos semelhantes ao controle positivo, corroborando seu potencial de toxicidade para o DNA. Portanto, conclui-se que o MT, em sua composição pura e nas concentrações realistas utilizadas, possui potencial genotóxico na avaliação biológica de células de A. cepa. A análise integrativa multivariada foi fundamental para mostrar uma resposta completa de todos os dados, fornecendo uma visão global do efeito da MT no DNA.


Assuntos
Humanos , Animais , Zika virus , Infecção por Zika virus , Inseticidas/toxicidade , Dano ao DNA , Aberrações Cromossômicas , Raízes de Plantas , Cebolas , Mosquitos Vetores , Malation/toxicidade , Índice Mitótico
2.
Biol. Models Res. Technol ; 2(1): e00022022, 2022.
Artigo em Inglês | VETINDEX | ID: biblio-1402379

Resumo

Arboviruses are worldwide distributed arthropod-borne viruses representing a constant threat to public health. Among these arboviruses, the Chikungunya (CHIKV) and Zika (ZIKV) viruses have a high prevalence in Brazil being responsible for recent outbreaks resulting mainly in irreparable socioeconomic damages such as the high rate of cases of comorbidities and microcephaly in newborns, respectively. Therefore, it is necessary to understand the biology of these arboviruses and develop effective treatments against them; moreover, appropriate mice animal models are strongly encouraged. Here we reviewed the scientific literature aiming to improve the search for the best murine animal model, specific for the arboviruses, specifically, CHIKV and ZIKV. In this way, we performed a comparison between the various mice models currently available, among them: genetically modified immunosuppressed animals, as the A129 and AG129 which are knockout animals for the α/ß and α/ß/γ receptors, respectively, neonatal immunocompetent models C57BL/6 strains used between 6-8 days old for neuropathogenesis studies or 1 day old for vaccine safety studies and finally immunosuppressed induced by dexamethasone or interferon 1 blocker for pathogenesis studies. Mice models are the first option after in vitro analysis, as they are small animals, which facilitates handling and maintenance, in addition to being more inexpensive and abundantly available in different genetic strains, both wild and modified. If the results of this stage are promising, the studies move forward to the use of models with animals of greater complexity, such as rats, non-human primates and finally humans. For this review, we searched through articles in PubMed, Scopus and ScienceDirect databases using the criteria of date publications, titles, abstracts and complete manuscripts. The correct choice of these models during experimental planning is essential, since increases the confidence and the rational use of animals in experimentation in accordance to current bioethics guidelines.


Assuntos
Animais , Camundongos , Vírus Chikungunya , Modelos Animais , Zika virus , Animais de Laboratório , Camundongos
3.
Arq. Inst. Biol ; 88: e00802019, 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1349009

Resumo

The present research evaluated the seroprevalence of anti-zika virus (anti-ZIKV) antibodies by virus neutralization test (VNT) in 529 bovines from Andradina city, São Paulo state, Brazil. The reading was performed in an inverted optical microscope, considering reagents when the antibodies were capable to neutralize the ZIKV. Of the 529 samples, 53 (10.01%) were reagents. The animals were healthy at the time of collection. The samples were collected in February 2018, a favorable period for the multiplication of the vector and the highest risk of disease transmission. None of the animals showed anti-bovine viral diarrhea virus (anti-BVDV) antibodies, ruling out a possible cross-reaction, reinforcing the possible contact of the bovine with the ZIKV. In the herd, 88 pregnant females were evaluated; of these, 12 cows were reactive, with no history of reproductive problems or fetal malformations. This is the first research on the seroprevalence of ZIKV in cattle in Brazil, and studies should continue to evaluate cattle as a possible host of this arbovirus and its possible consequences for unique health and agribusiness.


Assuntos
Animais , Bovinos , Bovinos , Estudos Soroepidemiológicos , Zika virus , Anticorpos , Vírus , Testes de Neutralização , Flavivirus , Indicadores e Reagentes
4.
J. Venom. Anim. Toxins incl. Trop. Dis. ; 26: e20200019, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-32336

Resumo

Zika virus (ZIKV), an emerging arthropod-borne virus (arbovirus) of the Flaviviridae family, is a current issue worldwide, particularly because of the congenital and neurological syndromes associated with infection by this virus. As the initial clinical symptoms of all diseases caused by this group are very similar, clinical diagnosis is difficult. Furthermore, laboratory diagnostic efforts have failed to identify specific and accurate tests for each virus of the Flaviviridae family due to the cross-reactivity of these viruses in serum samples. This situation has resulted in underreporting of the diseases caused by flaviviruses. However, many companies developed commercial diagnostic tests after the recent ZIKV outbreak. Moreover, health regulatory agencies have approved different commercial tests to extend the monitoring of ZIKV infections. Considering that a specific and sensitive diagnostic method for estimating risk and evaluating ZIKV propagation is still needed, this review aims to provide an update of the main commercially approved serological diagnostics test by the US Food and Drug Administration (FDA) and Brazilian National Health Surveillance Agency (ANVISA). Additionally, we present the technologies used for monoclonal antibody production as a tool for the development of diagnostic tests and applications of these antibodies in detecting ZIKV infections worldwide.(AU)


Assuntos
Infecção por Zika virus/diagnóstico , Testes Sorológicos , Comércio , Anticorpos Monoclonais/análise , Anticorpos Monoclonais/química , Zika virus
5.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 719-728, May-June, 2020. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1128887

Resumo

Este estudo investigou a toxicidade pré-natal do inseticida piriproxifeno em ratos Wistar, de forma a detectar possíveis alterações no desenvolvimento fetal da progênie exposta durante o período organogênico. Três doses de piriproxifeno (100, 300 e 500mg.kg-1) foram administradas por via oral às progenitoras, do sexto ao 15º dia de gestação. Os fetos foram submetidos à técnica de diafanização modificada descrita por Taylor e Van Dyke, para avaliação de malformações e alterações esqueléticas. Os resultados não demonstraram a ocorrência de toxicidade materna sistêmica ou alterações nos índices reprodutivos avaliados. Malformações ou alterações teratogênicas não foram detectadas, no entanto alterações esqueléticas sugestivas de retardo no desenvolvimento foram observadas especialmente nas doses mais altas testadas (300mg.kg-1 e 500mg.kg-1). Considerando-se a situação complexa de risco para a saúde humana, mostra-se importante a execução de investigações adicionais, de modo a contribuir para a adequada avaliação de risco do piriproxifeno em água potável.(AU)


This study investigated the prenatal toxicity of the insecticide pyriproxyfen in Wistar rats to detect the possible changes in the fetal development of the progeny exposed during the organogenic period. Three doses of pyriproxyfen (100, 300, and 500mg.kg-1) were administered orally to the progenitors, from day 6 to 15 of gestation. The fetuses were processed using the Taylor and Van Dyke modified diaphanization technique to evaluate malformations and skeletal changes. The results did not demonstrate the occurrence of systemic maternal toxicity or changes in the reproductive indexes evaluated. Malformations or teratogenic changes were not detected, however, skeletal changes suggestive of developmental delay were observed, especially in the highest doses tested (300 mg.kg-1 and 500 mg.kg-1). Owing to the potentially complex situation regarding its risk to human health, it is important that further studies be performed to contribute to the risk assessment of the addition of pyriproxyfen in drinking water.(AU)


Assuntos
Animais , Ratos , Praguicidas/efeitos adversos , Piridinas , Teratogênicos/análise , Desenvolvimento Fetal/efeitos dos fármacos , Ratos Wistar/embriologia , Zika virus , Microcefalia/veterinária
6.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 719-728, May-June, 2020. tab
Artigo em Inglês | VETINDEX | ID: vti-29871

Resumo

Este estudo investigou a toxicidade pré-natal do inseticida piriproxifeno em ratos Wistar, de forma a detectar possíveis alterações no desenvolvimento fetal da progênie exposta durante o período organogênico. Três doses de piriproxifeno (100, 300 e 500mg.kg-1) foram administradas por via oral às progenitoras, do sexto ao 15º dia de gestação. Os fetos foram submetidos à técnica de diafanização modificada descrita por Taylor e Van Dyke, para avaliação de malformações e alterações esqueléticas. Os resultados não demonstraram a ocorrência de toxicidade materna sistêmica ou alterações nos índices reprodutivos avaliados. Malformações ou alterações teratogênicas não foram detectadas, no entanto alterações esqueléticas sugestivas de retardo no desenvolvimento foram observadas especialmente nas doses mais altas testadas (300mg.kg-1 e 500mg.kg-1). Considerando-se a situação complexa de risco para a saúde humana, mostra-se importante a execução de investigações adicionais, de modo a contribuir para a adequada avaliação de risco do piriproxifeno em água potável.(AU)


This study investigated the prenatal toxicity of the insecticide pyriproxyfen in Wistar rats to detect the possible changes in the fetal development of the progeny exposed during the organogenic period. Three doses of pyriproxyfen (100, 300, and 500mg.kg-1) were administered orally to the progenitors, from day 6 to 15 of gestation. The fetuses were processed using the Taylor and Van Dyke modified diaphanization technique to evaluate malformations and skeletal changes. The results did not demonstrate the occurrence of systemic maternal toxicity or changes in the reproductive indexes evaluated. Malformations or teratogenic changes were not detected, however, skeletal changes suggestive of developmental delay were observed, especially in the highest doses tested (300 mg.kg-1 and 500 mg.kg-1). Owing to the potentially complex situation regarding its risk to human health, it is important that further studies be performed to contribute to the risk assessment of the addition of pyriproxyfen in drinking water.(AU)


Assuntos
Animais , Ratos , Praguicidas/efeitos adversos , Piridinas , Teratogênicos/análise , Desenvolvimento Fetal/efeitos dos fármacos , Ratos Wistar/embriologia , Zika virus , Microcefalia/veterinária
7.
J. venom. anim. toxins incl. trop. dis ; 26: e20200019, 2020. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1135144

Resumo

Zika virus (ZIKV), an emerging arthropod-borne virus (arbovirus) of the Flaviviridae family, is a current issue worldwide, particularly because of the congenital and neurological syndromes associated with infection by this virus. As the initial clinical symptoms of all diseases caused by this group are very similar, clinical diagnosis is difficult. Furthermore, laboratory diagnostic efforts have failed to identify specific and accurate tests for each virus of the Flaviviridae family due to the cross-reactivity of these viruses in serum samples. This situation has resulted in underreporting of the diseases caused by flaviviruses. However, many companies developed commercial diagnostic tests after the recent ZIKV outbreak. Moreover, health regulatory agencies have approved different commercial tests to extend the monitoring of ZIKV infections. Considering that a specific and sensitive diagnostic method for estimating risk and evaluating ZIKV propagation is still needed, this review aims to provide an update of the main commercially approved serological diagnostics test by the US Food and Drug Administration (FDA) and Brazilian National Health Surveillance Agency (ANVISA). Additionally, we present the technologies used for monoclonal antibody production as a tool for the development of diagnostic tests and applications of these antibodies in detecting ZIKV infections worldwide.(AU)


Assuntos
Vigilância Sanitária , Testes Sorológicos/métodos , Flaviviridae , Flavivirus , Zika virus , Anticorpos , Anticorpos Monoclonais
8.
J. venom. anim. toxins incl. trop. dis ; 25: e20190013, 2019. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1020024

Resumo

In Brazil and in other tropical areas Zika virus infection was directly associated with clinical complications as microcephaly in newborn children whose mothers were infected during pregnancy and the Guillain-Barré syndrome in adults. Recently, research has been focused on developing new vaccines and drug candidates against Zika virus infection since none of those are available. In order to contribute to vaccine and drug development efforts, it becomes important the understanding of the molecular basis of the Zika virus recognition, infection and blockade. To this purpose, it is essential the structural determination of the Zika virus proteins. The genome sequencing of the Zika virus identified ten proteins, being three structural (protein E, protein C and protein prM) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). Together, these proteins are the main targets for drugs and antibody recognition. Here we examine new discoveries on high-resolution structural biology of Zika virus, observing the interactions and functions of its proteins identified via state-of-art structural methodologies as X-ray crystallography, nuclear magnetic resonance spectroscopy and cryogenic electronic microscopy. The aim of the present study is to contribute to the understanding of the structural basis of Zika virus infection at an atomic level and to point out similarities and differences to others flaviviruses.(AU)


Assuntos
Preparações Farmacêuticas , Vacinas , Espectroscopia de Ressonância Magnética , Zika virus , Infecção por Zika virus , Cristalografia por Raios X
9.
J. Venom. Anim. Toxins incl. Trop. Dis. ; 25: e.20190013, Dec. 2, 2019. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-24702

Resumo

In Brazil and in other tropical areas Zika virus infection was directly associated with clinical complications as microcephaly in newborn children whose mothers were infected during pregnancy and the Guillain-Barré syndrome in adults. Recently, research has been focused on developing new vaccines and drug candidates against Zika virus infection since none of those are available. In order to contribute to vaccine and drug development efforts, it becomes important the understanding of the molecular basis of the Zika virus recognition, infection and blockade. To this purpose, it is essential the structural determination of the Zika virus proteins. The genome sequencing of the Zika virus identified ten proteins, being three structural (protein E, protein C and protein prM) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). Together, these proteins are the main targets for drugs and antibody recognition. Here we examine new discoveries on high-resolution structural biology of Zika virus, observing the interactions and functions of its proteins identified via state-of-art structural methodologies as X-ray crystallography, nuclear magnetic resonance spectroscopy and cryogenic electronic microscopy. The aim of the present study is to contribute to the understanding of the structural basis of Zika virus infection at an atomic level and to point out similarities and differences to others flaviviruses.(AU)


Assuntos
Zika virus/ultraestrutura , Proteínas Virais/ultraestrutura , Elementos Estruturais de Proteínas , Genoma Viral , Espectroscopia de Ressonância Magnética
10.
Zoologia (Curitiba, Impr.) ; 36: e.36272, Apr. 18, 2019.
Artigo em Inglês | VETINDEX | ID: biblio-1504559

Resumo

Emerging Infectious Diseases (EID) pose a world-wide health and socio-economic threat. Accelerating climate change and globalization are exposing unforeseen ways that pathogens cope with their surroundings. The 2015 Zika virus (ZIKV) outbreak was an example of expansion into previously inaccessible fitness spaces, causing a sudden epidemic. Recent studies indicating the subsequent decrease in symptomatic cases means the virus is in remission, currently poses little threat, and therefore can be ignored. We present an evolutionary scenario derived from the Stockholm Paradigm, of oscillating phases of expansion and isolation, accompanied by changes in transmission, persistence, virulence, and pathology. Chief among these is the likelihood that asymptomatic strains are constantly transmitted sexually. This suggests that the currently quiescent virus retains capacities to reemerge abruptly and spread rapidly in an arena of changing opportunity.


Assuntos
Doenças Transmissíveis Emergentes/patologia , Doenças Transmissíveis Emergentes/transmissão , Doenças Transmissíveis Emergentes/virologia , Surtos de Doenças , Zika virus/patogenicidade , América do Sul/epidemiologia , Virulência
11.
Zoologia (Curitiba) ; 36: e.36272, Dec. 5, 2019.
Artigo em Inglês | VETINDEX | ID: vti-24609

Resumo

Emerging Infectious Diseases (EID) pose a world-wide health and socio-economic threat. Accelerating climate change and globalization are exposing unforeseen ways that pathogens cope with their surroundings. The 2015 Zika virus (ZIKV) outbreak was an example of expansion into previously inaccessible fitness spaces, causing a sudden epidemic. Recent studies indicating the subsequent decrease in symptomatic cases means the virus is in remission, currently poses little threat, and therefore can be ignored. We present an evolutionary scenario derived from the Stockholm Paradigm, of oscillating phases of expansion and isolation, accompanied by changes in transmission, persistence, virulence, and pathology. Chief among these is the likelihood that asymptomatic strains are constantly transmitted sexually. This suggests that the currently quiescent virus retains capacities to reemerge abruptly and spread rapidly in an arena of changing opportunity.(AU)


Assuntos
Doenças Transmissíveis Emergentes/patologia , Doenças Transmissíveis Emergentes/transmissão , Doenças Transmissíveis Emergentes/virologia , Zika virus/patogenicidade , Surtos de Doenças , América do Sul/epidemiologia , Virulência
12.
Rev. Inst. Adolfo Lutz ; 77: e1760, 2018. map
Artigo em Português | LILACS, VETINDEX | ID: biblio-1489587

Resumo

Implementation of a geospatial surveillance and response system data resource for vector borne disease in the Americas (GeoHealth) will be tested using NASA satellite data, geographic information systems and ecological niche modeling to characterize the environmental suitability and potential for spread of endemic and epizootic vector borne diseases. The initial focus is on developing prototype geospatial models for visceral leishmaniasis, an expanding endemic disease in Latin America, and geospatial models for dengue and other Aedes aegypti borne arboviruses (zika, chikungunya), emerging arboviruses with potential for epizootic spread from Latin America and the Caribbean and establishment in North America. Geospatial surveillance and response system open resource data bases and models will be made available, with training courses, to other investigators interested in mapping and modeling other vector borne diseases in the western hemisphere and contributing brokered data to an expanding GeoHealth data resource as part of the NASA AmeriGEOSS initiative.


A implementação de uma fonte de dados de vigilância e um sistema de resposta geoespacial para doenças transmitidas por vetores nas Américas (GeoHealth) será testada utilizando dados provenientes de satélites da NASA, sistemas de informações geográficas e modelagem do nicho ecológico, para caracterizar a suceptibilidade ambiental e o potencial de dispersão de doenças endêmicas e epizooticas transmitidas por vetores vetores. O foco inicial será o desenvolvimento de protótipos de modelos geoespaciais para a leishmaniose visceral, uma doença endêmica e em expansão na América Latina, e modelos geoespaciais para dengue e outros transmitidos pelo Aedes aegypti (zika, chikungunya), arbovírus emergentes com potencial para disseminação epizoótica pela América Latina e Caribe e estabelecimento na América do Norte. Sistemas de vigilância e resposta geoespacial e modelos de recursos em bases de dados abertas serão diponibilizados, com cursos de treinamento, para outros pesquisadores interessados em mapear e modelar outras doenças transmitidas por vetores no hemisfério ocidental e contribuir intermediando dados para uma fonte de dados GeoHealth em expansão, como parte da Iniciativa AmeriGEOSS, da NASA.


Assuntos
Leishmaniose Visceral/prevenção & controle , Mapeamento Geográfico , Sistemas de Informação Geográfica , Aedes , América , Estados Unidos , United States National Aeronautics and Space Administration , Zika virus
13.
Braz. J. Microbiol. ; 49(1): 144-147, jan.-mar. 2018. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-18546

Resumo

Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.(AU)


Assuntos
Humanos , Zika virus/isolamento & purificação , Infecção por Zika virus/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real , Eletroforese em Gel de Ágar , Monitoramento Epidemiológico
14.
R. Inst. Adolfo Lutz ; 77: e1760, 2018. mapas
Artigo em Português | VETINDEX | ID: vti-24877

Resumo

Implementation of a geospatial surveillance and response system data resource for vector borne disease in the Americas (GeoHealth) will be tested using NASA satellite data, geographic information systems and ecological niche modeling to characterize the environmental suitability and potential for spread of endemic and epizootic vector borne diseases. The initial focus is on developing prototype geospatial models for visceral leishmaniasis, an expanding endemic disease in Latin America, and geospatial models for dengue and other Aedes aegypti borne arboviruses (zika, chikungunya), emerging arboviruses with potential for epizootic spread from Latin America and the Caribbean and establishment in North America. Geospatial surveillance and response system open resource data bases and models will be made available, with training courses, to other investigators interested in mapping and modeling other vector borne diseases in the western hemisphere and contributing brokered data to an expanding GeoHealth data resource as part of the NASA AmeriGEOSS initiative.(AU)


A implementação de uma fonte de dados de vigilância e um sistema de resposta geoespacial para doenças transmitidas por vetores nas Américas (GeoHealth) será testada utilizando dados provenientes de satélites da NASA, sistemas de informações geográficas e modelagem do nicho ecológico, para caracterizar a suceptibilidade ambiental e o potencial de dispersão de doenças endêmicas e epizooticas transmitidas por vetores vetores. O foco inicial será o desenvolvimento de protótipos de modelos geoespaciais para a leishmaniose visceral, uma doença endêmica e em expansão na América Latina, e modelos geoespaciais para dengue e outros transmitidos pelo Aedes aegypti (zika, chikungunya), arbovírus emergentes com potencial para disseminação epizoótica pela América Latina e Caribe e estabelecimento na América do Norte. Sistemas de vigilância e resposta geoespacial e modelos de recursos em bases de dados abertas serão diponibilizados, com cursos de treinamento, para outros pesquisadores interessados em mapear e modelar outras doenças transmitidas por vetores no hemisfério ocidental e contribuir intermediando dados para uma fonte de dados GeoHealth em expansão, como parte da Iniciativa AmeriGEOSS, da NASA.(AU)


Assuntos
Sistemas de Informação Geográfica , Mapeamento Geográfico , Leishmaniose Visceral/prevenção & controle , Aedes , Zika virus , United States National Aeronautics and Space Administration , Estados Unidos , América
15.
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-954845

Resumo

Viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. Yellow fever virus, H1N1 virus, HIV, dengue virus, hepatitis B and C are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. In this context, a recently introduced flavivirus in South America, called Zika virus (ZIKV), led the WHO to declare in February 1st 2016 a warning on Public Health Emergency of International Concern (PHEIC). ZIKV is an arbovirus of the Flaviviridae family firstly isolated from sentinels Rhesus sp. monkeys at the Ziika forest in Uganda, Africa, in 1947. Lately, the virus has well adapted to the worldwide spread Aedes aegypti mosquito, the vector for DENV, CHIKV, YFV and many others. At first, it was not considered a threat to human health, but everything changed when a skyrocketing number of babies born with microcephaly and adults with Guillain-Barré syndrome were reported, mainly in northeastern Brazil. It is now well established that the virus is responsible for the so called congenital Zika syndrome (CZS), whose most dramatic features are microcephaly, arthrogryposis and ocular damage. Thus, in this review, we provide a brief discussion of these main clinical aspects of the CZS, correlating them with the experimental animal models described so far.(AU)


Assuntos
Artrogripose , Aedes , Zika virus , Microcefalia , Modelos Teóricos
16.
Artigo em Inglês | VETINDEX | ID: vti-33006

Resumo

Viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. Yellow fever virus, H1N1 virus, HIV, dengue virus, hepatitis B and C are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. In this context, a recently introduced flavivirus in South America, called Zika virus (ZIKV), led the WHO to declare in February 1st 2016 a warning on Public Health Emergency of International Concern (PHEIC). ZIKV is an arbovirus of the Flaviviridae family firstly isolated from sentinels Rhesus sp. monkeys at the Ziika forest in Uganda, Africa, in 1947. Lately, the virus has well adapted to the worldwide spread Aedes aegypti mosquito, the vector for DENV, CHIKV, YFV and many others. At first, it was not considered a threat to human health, but everything changed when a skyrocketing number of babies born with microcephaly and adults with Guillain-Barré syndrome were reported, mainly in northeastern Brazil. It is now well established that the virus is responsible for the so called congenital Zika syndrome (CZS), whose most dramatic features are microcephaly, arthrogryposis and ocular damage. Thus, in this review, we provide a brief discussion of these main clinical aspects of the CZS, correlating them with the experimental animal models described so far.(AU)


Assuntos
Artrogripose , Aedes , Zika virus , Microcefalia , Modelos Teóricos
17.
Colloq. Agrar ; 13(1): 01-06, jan.-abr 2017. ilus, tab
Artigo em Português | VETINDEX | ID: biblio-1481337

Resumo

Este trabalho tem o objetivo de avaliar a eficácia de produtos parasiticidas no controle de larvas de Aedes aegypti e Culex quinquefasciatus. Os produtos Fluazuron 2,5g, Cipermetrina 150g, Flumetrina 1g, Propoxur 1g, Deltametrina 25g, Clorpirifós 30g, Cipermetrina 15g, Fenthion 15g, Diazinon técnico estabilizado 40g, Fipronil 2,5%, Triclorfone 97g, Coumafós 3g, Propoxur 2g, Fentione 15g, Amitraz 12,5% foram preparados conforme recomendações do fabricante, sendo aplicados em recipientes contendo larvas de A. aegypti e C. quinquefasciatus em ambiente controlado. A inspeção da viabilidade larval foi verificada durante 24 horas. Estes mesmos produtos foram aplicados nos locais de coleta das larvas, sendo os criadouros dos mosquitos vistoriados diariamente durante 30 dias. Foi observado que 100% das larvas morreram em até 15 minutos, tanto nos recipientes em laboratório quanto no meio ambiente. Nas áreas onde ocorreu a pulverização não se observou presença de mosquitos adultos ou larvas por um período de até 24 dias, dependendo do produto utilizado, da incidência solar e da precipitação local. Nos locais ao abrigo da chuva e luz solar intensa, a ausência de larvas dos mosquitos ocorreu por 30 dias. Podemos concluir com esses resultados que diferentes produtos utilizados em animais como parasiticidas externos foram eficazes contra larvas de culicideos, constituindo-se numa importante ferramenta para controle desses vetores.


This study aims to evaluate the effectiveness of parasiticides products in the control of Aedes aegypti and Culex quinquefasciatus larvae. The products Fluazuron 2,5g, Cipermetrina 150g, Flumetrina 1g, Propoxur 1g, Deltametrina 25g, Clorpirifós 30g, Cipermetrina 15g, Fenthion 15g, Diazinon técnico estabilizado 40g, Fipronil 2,5%, Triclorfone 97g, Coumafós 3g, Propoxur 2g, Fentione 15g, Amitraz 12,5% were prepared according to manufacturer's recommendations, being applied in containers containing larvae of A. aegypti and C. quinquefasciatus in a controlled environment. Inspection of the larval viability was assessed 24 hours. These same products have been applied in the collection sites of the larvae, and the breeding of mosquitoes inspected daily for 30 days. Was observed that 100% of the larvae died within 15 minutes, so the containers in the laboratory and into the environment. In areas where spraying was not observed the presence of mosquito adults or larvae for a period of up to 24 days depending on the product used, the location of sunlight and precipitation. In protected sites of rain and strong sunlight, the absence of mosquito larvae occurred for 30 days. We can conclude from these results that different products used in animals as external parasiticides were effective against culicides larvae, constituting an important tool in the control of these vectors.


Assuntos
Animais , Controle de Mosquitos , Dengue , Febre Amarela , Febre de Chikungunya , Inseticidas , Zika virus , Controle de Pragas , Mosquitos Vetores
18.
Colloq. agrar. ; 13(1): 01-06, jan.-abr 2017. ilus, tab
Artigo em Português | VETINDEX | ID: vti-734909

Resumo

Este trabalho tem o objetivo de avaliar a eficácia de produtos parasiticidas no controle de larvas de Aedes aegypti e Culex quinquefasciatus. Os produtos Fluazuron 2,5g, Cipermetrina 150g, Flumetrina 1g, Propoxur 1g, Deltametrina 25g, Clorpirifós 30g, Cipermetrina 15g, Fenthion 15g, Diazinon técnico estabilizado 40g, Fipronil 2,5%, Triclorfone 97g, Coumafós 3g, Propoxur 2g, Fentione 15g, Amitraz 12,5% foram preparados conforme recomendações do fabricante, sendo aplicados em recipientes contendo larvas de A. aegypti e C. quinquefasciatus em ambiente controlado. A inspeção da viabilidade larval foi verificada durante 24 horas. Estes mesmos produtos foram aplicados nos locais de coleta das larvas, sendo os criadouros dos mosquitos vistoriados diariamente durante 30 dias. Foi observado que 100% das larvas morreram em até 15 minutos, tanto nos recipientes em laboratório quanto no meio ambiente. Nas áreas onde ocorreu a pulverização não se observou presença de mosquitos adultos ou larvas por um período de até 24 dias, dependendo do produto utilizado, da incidência solar e da precipitação local. Nos locais ao abrigo da chuva e luz solar intensa, a ausência de larvas dos mosquitos ocorreu por 30 dias. Podemos concluir com esses resultados que diferentes produtos utilizados em animais como parasiticidas externos foram eficazes contra larvas de culicideos, constituindo-se numa importante ferramenta para controle desses vetores.(AU)


This study aims to evaluate the effectiveness of parasiticides products in the control of Aedes aegypti and Culex quinquefasciatus larvae. The products Fluazuron 2,5g, Cipermetrina 150g, Flumetrina 1g, Propoxur 1g, Deltametrina 25g, Clorpirifós 30g, Cipermetrina 15g, Fenthion 15g, Diazinon técnico estabilizado 40g, Fipronil 2,5%, Triclorfone 97g, Coumafós 3g, Propoxur 2g, Fentione 15g, Amitraz 12,5% were prepared according to manufacturer's recommendations, being applied in containers containing larvae of A. aegypti and C. quinquefasciatus in a controlled environment. Inspection of the larval viability was assessed 24 hours. These same products have been applied in the collection sites of the larvae, and the breeding of mosquitoes inspected daily for 30 days. Was observed that 100% of the larvae died within 15 minutes, so the containers in the laboratory and into the environment. In areas where spraying was not observed the presence of mosquito adults or larvae for a period of up to 24 days depending on the product used, the location of sunlight and precipitation. In protected sites of rain and strong sunlight, the absence of mosquito larvae occurred for 30 days. We can conclude from these results that different products used in animals as external parasiticides were effective against culicides larvae, constituting an important tool in the control of these vectors.(AU)


Assuntos
Animais , Dengue , Febre Amarela , Febre de Chikungunya , Zika virus , Inseticidas , Controle de Mosquitos , Controle de Pragas , Mosquitos Vetores
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