Penile fractures in young bulls raised in feedlots in Southern Brazil

Background: Penile fracture is a pathology of young cattle that perform precocious and disordered breeding. The incompatibility of height between males and females and sodomy between males cause a great pressure on the sigmoid flexure and retractor muscle of the penis, which are the main causes and sites of organ injury. This study aimed to describe the epidemiological and pathological aspects of penile fractures observed in young bulls raised in pre-export feedlots (PEFs) in southern Brazil. Cases: In 2 PEFs located in the municipalities of Pelotas (property 1) and Capão do Leão (property 2), 3 male cattle [1 from property 1 and 2 from property 2] presented subcutaneous edema in the foreskin and perineum, associated with dysuria. The evolution of the clinical picture was approximately 20 days in all cases, with evolution to death. The bovine necropsied on property 1 had an increased volume and inguinal edema, involving the penis and scrotal sac. Necrosis of the subcutaneous tissue and local musculature was also observed. The testicles were surrounded by the necrotic tissue, and the right testis was swollen, with flaccid parenchyma adhering to the tunica albuginea. In the necropsy of 1 bull from property 2, an increase in the inguinal volume was observed, with an extensive area of necrosis and edema extending from the prepuce to the caudal musculature of the scrotal sac. There were also marked varicosis in the sigmoid flexure and necrosis of the adjacent region, without the involvement of the corpus cavernosum. During the necropsy of the 2 young bulls, fragments of organs from the abdominal, thoracic, and brain cavities were collected and fixed in 10% buffered formalin. From the bull of the property 2, an anatomical piece consisting of the penis, prepuce, and testicles was also collected and fixed in 10% buffered formalin. After 48 h, the tissue samples were cleaved, embedded in paraffin, cut into 3-µm-thick sections, and stained using hematoxylin and eosin (HE). A histological evaluation of the penile lesions in both cattle revealed intense hemorrhage, congestion, and necrosis of the muscles and tissues adjacent to the corpus cavernosum. In addition to areas of dystrophic calcification, neutrophil and macrophage infiltration was also observed. In the bull from the property 1, an intense edema and proliferation of fibrous tissue surrounding the urethra were noted. There were also marked tubular degeneration and intense infiltration of neutrophils, lymphocytes, and macrophages in the inner portion of the tunica albuginea. Discussion: In the present cases, the diagnosis was based on epidemiological data associated with clinical signs and pathology. The macroscopic lesions observed were probably due to the involvement of blood vessels adjacent to the penis, which suffered trauma during sodomy mating among cattle. These lesions have been described in other reports of this pathology and in diseases, such as acropostitis-phimosis, fibropapilloma of the glans, preputial abscess, and urolithiasis, and the differential diagnosis of these diseases must be carried out, as they have different etiologies. In the bulls of the present study, no lesions were observed in the corpus cavernosum, and this condition was attributed to the presence of varicosis and accumulation of urine in the prepuce, due to the difficulty in exposing the penis. Histologically, there were intense hemorrhage, congestion, and necrosis of the muscles and tissues adjacent to the corpus cavernosum, with the infiltration of neutrophils and macrophages, and areas of dystrophic calcification. The presence of necrotic lesions in tissues adjacent to the penis may be related to hypoxia, vascular lesions, or the action of chemical elements present in the urine. In both cases, vascular lesions were present, which were attributed to the main triggering factor for the disease.

Toxoplasmose encefálica em um gambá-de-orelha-branca (Didelphis albiventris) / Encephalic toxoplasm in a white-eared possum (Didelphis albiventris)

Background: Toxoplasmosis is caused by Toxoplasma gondii, an obligate intracellular protozoan that belongs to the Apicomplexa phylum, coccidian subclass, and affects all warm-blooded animals. The role of opossums in the epidemiology of toxoplasmosis in Brazil is not fully understood, and there are very few descriptions of toxoplasmosis lesions in these animals. This report describes the anatomopathological, molecular and immunohistochemical findings of a case of encephalic toxoplasmosis in free-living white-eared possum (Didelphis albiventris). Case: A young male opossum (D. albiventris), was treated at the Veterinary Hospital of Wild Animals of the University of Brasília, Federal District. The animal was apathetic, uncoordinated, reluctant to move, and had an exposed proximal fracture in the left radius and ulna with laceration of muscles and adjacent tendinous structures. Amputation on the left thoracic limb was performed followed by analgesia and antibiotic therapy. The environment is frequented by other wild animals, and stray cats have access to the patio of the building. Twenty-five days after arriving at the hospital, the animal was found dead in its cage. After death, a necropsy was performed. Organ fragments from the abdominal cavity, thoracic and central nervous system were collected, processed routinely for histology and stained with hematoxylin and eosin. Macroscopic lesions in the central nervous system were not observed. On microscopy, the brain showed moderate random glial nodules throughout the neuropil associated with the presence of spherical to elongated parasitic cysts of about 20 µm, with a thin wall and with its interior full of bradyzoites, consistent with Toxoplasma gondii. There was also moderate fibrinoid necrosis and moderate multifocal lymphoplasmacytic infiltrate surrounding the blood vessels (perivascular cuffs) To investigate the etiology of the brain injury, brain sections were subjected to immunohistochemistry (IHC) and real-time polymerase chain reaction (qPCR) technique for detection of T. gondii and Neospora caninum. Immunostaining for T. gondii in the cyst wall and in bradyzoites and negative immunostaining for N. caninum. qPCR was positive for T. gondii and negative for N. caninum. Discussion: Diagnosis of encephalic toxoplasmosis in a Didelphis albiventris was possible based on histopathological, immunohistochemical and molecular findings. The morphological classification of the brain lesion was important for the diagnosis. Brain toxoplasmosis in opossums usually results in focal areas of malacia on macroscopy and focally extensive necrosis on microscopy, neutrophil infiltrate, calcified necrotic material, and perivascular cuffs of lymphocytes and plasma cells. In the present case, similar histopathological lesions were noted, but no significant macroscopic changes were observed. The etiology here was defined by immunohistochemistry and qPCR, techniques proven to be useful and with good specificity for diagnosing toxoplasmosis in mammals. It is believed that the positive immunohistochemical and molecular result for Toxoplasma gondii together with the negative result for Neospora caninum were conclusive for the diagnosis. Thus, we demonstrate here a post mortem diagnosis of toxoplasmosis in a free-living synanthropic opossum and the use of anatomopathology, immunohistochemistry and real-time polymerase chain reaction as a diagnostic option for this disease in opossums.

Effects of Different Amounts of Blue Lupine (L. Angustifolius L.) in The Diets of Heavy-Type Turkeys on Their Growth Rate, Carcass and Meat Qualities

A study was carried out to determine the effects of soybean meal replacement with different amounts of blue lupine in the diets of turkeys on the growth rate, anatomic carcass dissection data, chemical indicators of breast and thigh muscles and the content of tryptophan and oxyprolin. In total three hundred and sixty cross BIG-6 turkeys were allotted to two control and ten experimental groups of 30 one-day-old turkeys. The control group of turkeys was fed the diet containing soybean meal, whereas the trial groups were offered different amounts (from 20 to 30%) of lupines. Group 4 and 5 were additionally given probiotic mixture Bio Plus 2B and allzyme SSF, respectively. Soybean oil replacement from 20 to 30% lupine in the diet had no influence on the growth rate, dressing percentage, edible parts and abdominal fat content of turkeys. The study indicated that lupines in the diet of turkeys had a different effect on the meat quality of different genders. Lupines did not have any negative effect on the meat quality of female turkeys and 30% lupines even improved the protein value index of breast muscles. However, 20-30 and 25-30% lupines in male turkey diets lowered dry matter and protein contents in breast muscles but had no negative influence on the main quality indicators in thigh muscles. The results of the study showed that the negative effect on the male breast muscle quality might be avoided using Bio Plus 2B or allzyme SSF additives in the male diets containing 30% lupines.(AU)

Effects of Different Amounts of Blue Lupine (L. Angustifolius L.) in The Diets of Heavy-Type Turkeys on Their Growth Rate, Carcass and Meat Qualities

A study was carried out to determine the effects of soybean meal replacement with different amounts of blue lupine in the diets of turkeys on the growth rate, anatomic carcass dissection data, chemical indicators of breast and thigh muscles and the content of tryptophan and oxyprolin. In total three hundred and sixty cross BIG-6 turkeys were allotted to two control and ten experimental groups of 30 one-day-old turkeys. The control group of turkeys was fed the diet containing soybean meal, whereas the trial groups were offered different amounts (from 20 to 30%) of lupines. Group 4 and 5 were additionally given probiotic mixture Bio Plus 2B and allzyme SSF, respectively. Soybean oil replacement from 20 to 30% lupine in the diet had no influence on the growth rate, dressing percentage, edible parts and abdominal fat content of turkeys. The study indicated that lupines in the diet of turkeys had a different effect on the meat quality of different genders. Lupines did not have any negative effect on the meat quality of female turkeys and 30% lupines even improved the protein value index of breast muscles. However, 20-30 and 25-30% lupines in male turkey diets lowered dry matter and protein contents in breast muscles but had no negative influence on the main quality indicators in thigh muscles. The results of the study showed that the negative effect on the male breast muscle quality might be avoided using Bio Plus 2B or allzyme SSF additives in the male diets containing 30% lupines.

Neuromiopatia isquêmica em cinco cães (2008-2016) / Ischemic neuromyopathy in five dogs (2008-2016)

O objetivo deste estudo foi apresentar dados epidemiológicos, clínicos e anatomopatológicos de cinco cães com neuromiopatia isquêmica secundária a trombose ou tromboembolismo aórtico. Os cães eram fêmeas, com idade ≥11 anos, que apresentavam paraplegia (80%) ou monoplegia (20%) de membro pélvico. Em 40% dos casos (n=2) foi relatada claudicação previamente à disfunção motora. Os principais achados clínicos foram ausência de pulso femoral uni ou bilateral, extremidade de membros pélvicos frias, dor muscular acima da articulação do joelho e ausência de nocicepção (dor profunda) nos dígitos dos membros pélvicos. Quatro cães foram submetidos à eutanásia devido ao prognóstico desfavorável e um veio a óbito em menos de 24 horas de internação. Na necropsia foi encontrada alteração cardíaca em três cães, neoplásica em um e, sem causa determinante em outro caso. Os trombos estavam localizados na aorta abdominal e se estendiam até as artérias ilíacas. Mesmo sendo baixa a incidência, a neuromiopatia isquêmica deve ser incluída no diagnóstico diferencial de cães com claudicação, paraparesia ou paraplegia uni ou bilateral, quando os sinais neurológicos são compatíveis com lesão de neurônio motor inferior (L4-S3) e de evolução aguda ou crônica. A avaliação do pulso femoral digital e/ou por doppler vascular deve ser empregada e pode auxiliar no diagnóstico da doença evitando que os animais iniciem tratamento para outras afecções.(AU)

This study aimed to present epidemiological, clinical and anatomopathological data of five dogs with ischemic neuromyopathy secondary to aortic thrombosis or thromboembolism. The dogs were females, with eleven years of age or more, that had paraplegia (80%) or hind limb monoplegia (20%). Forty percent of the cases (n=2) had lameness previously the motor disfunction. The main clinical findings include absence of unilateral or bilateral femoral pulse, cool hind limbs extremities, muscle pain above the knee joint and absence of nociception (deep pain) on the hind limbs digits. Four dogs were euthanized due to the poor prognosis, and the last one died within 24 hours of hospital admission. The necropsy findings were cardiac lesions in three dogs, neoplastic disease in one, and unknown cause on the other. The thrombi were located on the abdominal aorta, extending to the iliac arteries. Even with low incidence, the ischemic neuromyopathy should be included in the differential diagnosis of dogs with lameness, paraparesis, or unilateral or bilateral paraplegia, when neurological signs are compatible with lower motor neuron lesion (L4-S3) and acute or chronic progression. The digital and/or vascular doppler evaluation of the femoral pulse must be used and can assist on the disease diagnosis, avoiding treatments for other illnesses.(AU)

Neuromiopatia isquêmica em cinco cães (2008-2016) / Ischemic neuromyopathy in five dogs (2008-2016)

O objetivo deste estudo foi apresentar dados epidemiológicos, clínicos e anatomopatológicos de cinco cães com neuromiopatia isquêmica secundária a trombose ou tromboembolismo aórtico. Os cães eram fêmeas, com idade ≥11 anos, que apresentavam paraplegia (80%) ou monoplegia (20%) de membro pélvico. Em 40% dos casos (n=2) foi relatada claudicação previamente à disfunção motora. Os principais achados clínicos foram ausência de pulso femoral uni ou bilateral, extremidade de membros pélvicos frias, dor muscular acima da articulação do joelho e ausência de nocicepção (dor profunda) nos dígitos dos membros pélvicos. Quatro cães foram submetidos à eutanásia devido ao prognóstico desfavorável e um veio a óbito em menos de 24 horas de internação. Na necropsia foi encontrada alteração cardíaca em três cães, neoplásica em um e, sem causa determinante em outro caso. Os trombos estavam localizados na aorta abdominal e se estendiam até as artérias ilíacas. Mesmo sendo baixa a incidência, a neuromiopatia isquêmica deve ser incluída no diagnóstico diferencial de cães com claudicação, paraparesia ou paraplegia uni ou bilateral, quando os sinais neurológicos são compatíveis com lesão de neurônio motor inferior (L4-S3) e de evolução aguda ou crônica. A avaliação do pulso femoral digital e/ou por doppler vascular deve ser empregada e pode auxiliar no diagnóstico da doença evitando que os animais iniciem tratamento para outras afecções.(AU)

This study aimed to present epidemiological, clinical and anatomopathological data of five dogs with ischemic neuromyopathy secondary to aortic thrombosis or thromboembolism. The dogs were females, with eleven years of age or more, that had paraplegia (80%) or hind limb monoplegia (20%). Forty percent of the cases (n=2) had lameness previously the motor disfunction. The main clinical findings include absence of unilateral or bilateral femoral pulse, cool hind limbs extremities, muscle pain above the knee joint and absence of nociception (deep pain) on the hind limbs digits. Four dogs were euthanized due to the poor prognosis, and the last one died within 24 hours of hospital admission. The necropsy findings were cardiac lesions in three dogs, neoplastic disease in one, and unknown cause on the other. The thrombi were located on the abdominal aorta, extending to the iliac arteries. Even with low incidence, the ischemic neuromyopathy should be included in the differential diagnosis of dogs with lameness, paraparesis, or unilateral or bilateral paraplegia, when neurological signs are compatible with lower motor neuron lesion (L4-S3) and acute or chronic progression. The digital and/or vascular doppler evaluation of the femoral pulse must be used and can assist on the disease diagnosis, avoiding treatments for other illnesses.(AU)

Analysis of effect isoxsuprine hydrochloride and nicotine in the Transverse Rectus Abdominis Myocutaneous flap (TRAM) in rats

PURPOSE: To evaluate the effects of isoxsuprine and nicotine on TRAM. METHODS: Forty eight 48 Wistar rats distributed into four Groups (n=12). All rats received medication managed daily for 20 days: saline solution (SA), nicotine solution (NI), isoxsuprine solution (IS) and nicotine solution (NI) + isoxsuprine solution (IS). On day 21st the rats were submitted to the caudally based, right unipedicled TRAM flap and after 48 hours, made the macroscopic evaluation of the surface of the flap, photographic documentation and collection of material for histology. Data from macroscopic evaluation were analyzed by ANOVA and microscopic evaluation by Kruskal-Wallis test, with significance level of 5%. RESULTS: In the macroscopic evaluation of isoxsuprine Group retail presented absolute numbers: final area (p=0.001*) and viable area (p=0.006*) with the highest values; necrosis (p=0.001*) had the lowest value. Microscopic examination revealed no significant findings in the study of TRAM under the action of isoxsuprine and nicotine to the percentage of necrosis in the left and right cranial and caudal regions. CONCLUSIONS: There was significant improvement in viability of TRAM using the isoxsuprine solution alone. No influence using nicotine alone and in association with isoxsuprine.(AU)

Ingestion of foreign body in green iguana (Iguana iguana) / Ingestão de corpo estranho em iguana verde (Iguana iguana)

Background: Green iguanas (Iguana iguana) are arboreal herbivorous species of lizards of the genus Iguana, native to Central and South America and Caribbean. Because demand for pet iguanas increased the incidence of problems related to sanitary handling and feeding of these animals also increased. Cases of atony caused by ingestion of foreign bodies, particularly of substratum can occur. This article aims to contribute to a better clarification of atonia in the Green Iguana that is caused by the ingestion of foreign bodies. Case: A 1-year-old female green iguana weighing 1,3 kg, was referred to the Veterinary Clinic Pet Fauna, Porto Alegre, RS, Brazil, on August 2012, with an apparent loss of appetite. The animal was apathetic, dehydrated, had a abdominal distension and low body score. Revealed an increase of aspartate aminotransferase (AST), monocytosis and decreased of mean corpuscular hemoglobin concentration (CAGH). Ozolaimus cirratus was found on parasitological examination of faecal sample. Ultrasound showed hyper echoic nodular structures in the region of the stomach and intestine and intestinal atony. Radiographs revealed radiopaque hyperdense areas in the topography of stomach and intestinal lumen, compatible with foreign body. The treatment fluid was set with 0.9% sodium chloride solution (50 mg/kg, SC, every 24 h), ceftazidime (20 mg/kg, IM, every 72 h)...(AU)

Ingestion of foreign body in green iguana (Iguana iguana) / Ingestão de corpo estranho em iguana verde (Iguana iguana)

Background: Green iguanas (Iguana iguana) are arboreal herbivorous species of lizards of the genus Iguana, native to Central and South America and Caribbean. Because demand for pet iguanas increased the incidence of problems related to sanitary handling and feeding of these animals also increased. Cases of atony caused by ingestion of foreign bodies, particularly of substratum can occur. This article aims to contribute to a better clarification of atonia in the Green Iguana that is caused by the ingestion of foreign bodies. Case: A 1-year-old female green iguana weighing 1,3 kg, was referred to the Veterinary Clinic Pet Fauna, Porto Alegre, RS, Brazil, on August 2012, with an apparent loss of appetite. The animal was apathetic, dehydrated, had a abdominal distension and low body score. Revealed an increase of aspartate aminotransferase (AST), monocytosis and decreased of mean corpuscular hemoglobin concentration (CAGH). Ozolaimus cirratus was found on parasitological examination of faecal sample. Ultrasound showed hyper echoic nodular structures in the region of the stomach and intestine and intestinal atony. Radiographs revealed radiopaque hyperdense areas in the topography of stomach and intestinal lumen, compatible with foreign body. The treatment fluid was set with 0.9% sodium chloride solution (50 mg/kg, SC, every 24 h), ceftazidime (20 mg/kg, IM, every 72 h)...

Infectious muscle necrosis etiology in the Pacific White Shrimp (Penaeus vannamei) cultured in Ecuador

In recent years, some Ecuadorian shrimp farmers have reported several cases of muscle necrosis in P. vannamei grow out cultures, associated to low mortalities at harvest. This abnormal condition is characterized by focal to extensive necrotic areas in striated tail muscle tissues, displaying a white, opaque appearance. Furthermore, histological analysis from suspected samples with macroscopic lesions revealed a loss of sarcomeric structure accompanied by coagulative muscle necrosis along with hemocytic infiltration. Two viruses, not reported in Ecuador, are described as etiological agents of muscle necrosis in P. vannamei: infectious myonecrosis virus (IMNV) and Penaeus vannamei nodavirus (PvNV). In this study, the etiology of the muscle necrosis cases found in the Pacific white shrimp P. vannamei cultured in Ecuador was examined. This muscle necrosis was hypothesized to be caused by an infectious agent. Three sequential challenge tests, using diseased P. vannamei with macroscopic lesions (opaque, whitish discolorations in the abdominal muscles) as starting material (crude inoculum), were carried out. Essentially, histological examination confirmed that most of the challenged shrimp had lesions in skeletal muscle, including multifocal necrosis, fibrocytic inflammation and phagocytosis. In the first trial, 7 challenged shrimp (out of 10) presented multifocal necrosis and hemocytic infiltration in the skeletal muscle. In the second trial, 14 challenged shrimp (out of 20) exhibited coagulative muscle necrosis and hemocytic infiltration. In the third trial, 48 challenged shrimp (out of 69) showed muscle necrosis and hemocytic infiltration. There were no mortalities throughout the all four-week trials. All challenged shrimp tested by RT-PCR assay were negative for IMNV. In our experimental procedures, muscle necrosis could be consistently reproduced through three sequential trials, confirming that the disease has an infectious etiology. Our results suggest that the etiological agent of this disease could be a new infectious agent or a different strain of IMNV.(AU)

QUALIDADE DA ÁGUA E DO PESCADO (TILÁPIA DO NILO - Oreochromis niloticus) ORIUNDOS DE PESQUE-PAGUES SITUADOS NO ESTADO DE SÃO PAULO

Os pesque-pagues constituem atividade recente, difundida por todo o Brasil, sendo uma opção de lazer, entretenimento e prestação de serviços, carecendo de adequado enquadramento regulamentar e suas necessárias salvaguardas. Estabelecimentos mal estruturados, irregulares, com manejo inadequado, sem supervisão técnica e fiscalização podem ser responsáveis por impactos ambientais e danos à saúde pública. Neste trabalho propôs-se avaliar a influência da variação sazonal, representada pela época da seca e época das chuvas, sobre a qualidade da água e do pescado (tilápia do Nilo Oreochromis niloticus) oriundos de cinco diferentes pesque-pagues localizados no Estado de São Paulo. Foram realizadas análises físicas e químicas (temperatura, oxigênio dissolvido e pH) e microbiológica da água de cultivo (coliformes termotolerantes), análise do tempo de instalação do pleno rigor-mortis nos exemplares de peixes e análises microbiológicas quantitativas (estimativas de microrganismos aeróbios mesófilos, de microrganismos aeróbios psicrotróficos e de bactérias dos gêneros Pseudomonas spp. e Aeromonas spp.) do limo superficial, das brânquias, da lavagem da cavidade abdominal, da musculatura com pele e dos filés de tilápias do Nilo. Verificou-se que houve diferença significativa (p<0,05) entre os períodos analisados para a temperatura, o oxigênio dissolvido e o pH da água de cultivo dos pesque-pagues. Para coliformes termotolerantes, 20% (4/20) dos tanques dos pesque-pagues foram considerados impróprios para produção de peixes no período das chuvas. Tanto para o limo superficial, quanto para as brânquias, observou-se reflexo dessa condição para, respectivamente, 10% (3/30) e 20% (6/30) com excesso de coliformes termotolerantes nos períodos da seca e das chuvas. Independente do período, após o abate por destruição cerebral verificou-se que o rigor-mortis atingiu sua plenitude cinco horas após a despesca. A maioria dos parâmetros avaliados, representados por estimativas microbiológicas do limo superficial, das brânquias, da lavagem da cavidade abdominal e da musculatura com pele, houve diferença significativa (p<0,05) entre os períodos da seca e das chuvas. Para o gênero Aeromonas spp. houve diferença significativa (P<0,05) somente no limo superficial. Para os filés observou-se, na quantificação dos mesófilos no tempo zero, a maior estimativa, tanto para o período das chuvas quanto para o da seca, ocorrendo redução com o passar das horas. Comportamento inverso ocorreu para os psicrotróficos e bactérias do gênero Pseudomonas spp., em concordância com a temperatura de estocagem e de classificação preferencial desses microrganismos. Bactérias do gênero Aeromonas spp. somente foram detectadas nas amostras de filés no período das chuvas. Conclui-se que o período das chuvas interferiu de forma negativa na qualidade da água e do pescado de pesque-pagues, mas, independente do período, são necessárias medidas higiênico-sanitárias preventivas com o intuito de assegurar a inocuidade do produto final.

The fish and pay constitute recent activity, widespread throughout Brazil, one option for leisure, entertainment and services, lacking adequate regulatory framework and its necessary safeguards. Barely establishments installed, irregular, with inadequate management without technical supervision and inspec tion may account for environmental impacts and damage to public health. In this work proposed to evaluate the influence of seasonal variation, represented by the dry season and the rainy season on water quality and fish (Nile tilapia Oreochromis niloticus) from five different fish and pay located in São Paulo. Physical and chemical analysis (temperature, dissolved oxygen and pH) were performed and microbiological cultivation of water (fecal coliform), analysis of the installation time of rigor mortis-full in specimens of fish and quantitative microbiological analysis (estimates of mesophilic aerobic microorganisms, of psychrotrophic aerobic microorganisms and bacteria of the genera Pseudomonas spp and Aeromonas spp.) of the surface slime, gills, of the abdominal cavity wash, skin and muscles with the Nile tilapia fillets. There was a significant difference (p <0.05) between the periods analyzed for temperature, dissolved oxygen and pH of the cultivation of water from fish and pay. For fecal coliforms, 20% (4/20) of the tanks of fish and pay were considered unsuitable for fish production during the rainy season. Both the surface slime, as to the gills, there was a reflection of that condition for, respectively, 10% (3/30) and 20% (6/30) with excess fecal coliform in periods of drought and rain. Regardless of the period after slaughter by brain destruction was found that the rigor-mortis reached its fullness five hours after is harvesting. Most of these parameters, represented by microbiological estimates of surface slime, gills, wash the abdominal cavity and the muscles with skin, there was a significant difference (p <0.05) between periods of drought and rain. To the genre Aeromonas spp. was no significant difference (P <0.05) only in the surface slime. For steaks observed, the quantification of mesophilic at time zero, the highest estimate for both the rainy season and for the drought, leading to a reduction in the hours passed. Opposite behavior occurred for psychrotrophic and bacteria of the genus Pseudomonas spp., In accordance with the temperature storage and preferential classification of microorganisms. Bacteria of the genus Aeromonas spp. They were only found in samples of steaks during the rainy season. We conclude that the rainy season interfered negatively on water quality and fish and pay of fish, but independent of the period, are necessary preventive hygienic-sanitary measures in order to ensure the safety of the final product.

Uroperitônio em três potros Puro Sangue de corrida / Uroperitoneum in three Thoroughbred toets / Uroperitoneo en tres potros Pura Sangre de Carrera

São descritos três casos de uroperitônio em duas potrancas e um potro Puro Sangue de Corrida de 12, 20 e 8 dias de idade, respectivamente. Os animais apresentavam sinais de dor abdominal leve à moderada, taquipneia,taquicardia, distensão abdominal e disúria. O diagnóstico de uroperitônio foi realizado por meio de ultrassonografia transabdominal onde se observou a presença de imagens ecográficas sugestivas de alças intestinais flutuando em grande quantidade de líquido hipoecoico; e confirmado pela relação creatinina do líquido abdominal: creatininas é rica acima de dois. Primeiramente os potros foram submetidos à fluido terapia intravenosa associada à drenagem progressiva do líquido peritoneal para posterior celiotomia exploratória com o objetivo de localizar e reparar a lesão.Na potranca de 20 dias e no potro de 8 dias foi identificada uma ruptura de úraco. Na potranca de 12 dias, foi constatado um defeito congênito na parede dorsal da bexiga que foi reparado após leve debridamento dos bordos da lesão. Nos três casos foi efetuada a ressecção do úraco, veia e artérias umbilicais e posterior cistorrexia. Os animais de 20, 8 e 12 dias de idade apresentaram uma melhora rápida e sem complicações após a correção cirúrgica,recebendo alta aos nove, sete e 23 dias após a intervenção, respectivamente.

Three cases of uroperitoneum in Thorough bred foals, two females and one male, are described in the present study. The animais showed clinical sings of mild to moderate abdominal pain, tachycardia, tachypnea,abdominal distention and straining to urinate. The diagnosis of uroperitoneum was made by transabdominal ultrasound examination and confirmed by the ratio of [abdominal fluid creatinine] : [serum creatinine] greater than two. Initially the foals were treated with intravenous fluid therapy associated with progressive drainage of peritoneal fluid forsubsequent exploratory laparotomy in order to identify and repair the lesion. A ruptured urachus was identified in the20-day-old filly and in the 8-day-old colt. The 12-day-old filly had a congenital defect in the dorsal aspect of thebladder; this defect was repaired after debridement of the lesion edges. The resection of urachus, umbilical vein andarteries was pertormed in ali three cases followed by cistorrhaphy. The outcome was good without complications aftersurgical correction; the 20-day old filly , 8-day-old colt and the 12-day-old filly were discharged at 9,7 and 23 daysafter surgery, respectively.

Tres casos de uroperitoneo son descriptos, respectivamente, en dos potrancas y un potro Pura Sangrede Carrera de 12, 20 Y 8 días de edad. Los animales presentaban senales de dolor abdominal discreta a moderada,taquipnea, taquicardia, distensión abdominal y disuria. EI diagnóstico de uroperitoneo fue realizado por medio de ultrasonografía transabdominal, donde fueron observadas vueltas intestinales fluctuando en gran cantidad de líquido hipoecogénico; fue confirmado por Ia relación creatinina dei líquido abdominal: creatinina sé rica arriba de 2. Primeramente los productos fueron sometidos a Ia fluidoterápia intravenosa asociada a un drenaje progresivo deilíquido peritoneal para una posterior quirurgia abdominal exploratoria con el objetivo de localizar y reparar Ia lesión.En Ia potranca de 20 días y en el potro de 8 días fue identificada una ruptura de uraco. En Ia potranca de 12 días fue constatada una alteración congénita en Ia pared vesical dorsal que fue reparada después de un desbridamiento delos bordos de Ia lesión. En los 3 casos fue efectuada Ia resección dei uraco, de Ias venas y arterias umbilicales e unaposterior cistorrafia. Los 3 animales de 20, 8 Y 12 días de edad presentaron una rápida mejora sin complicaciones después de Ia corrección quirúrgica, y recibieron alta 9, 7 Y 23 días después.

Uroperitônio em três potros Puro Sangue de corrida / Uroperitoneum in three Thoroughbred toets / Uroperitoneo en tres potros Pura Sangre de Carrera

São descritos três casos de uroperitônio em duas potrancas e um potro Puro Sangue de Corrida de 12, 20 e 8 dias de idade, respectivamente. Os animais apresentavam sinais de dor abdominal leve à moderada, taquipneia,taquicardia, distensão abdominal e disúria. O diagnóstico de uroperitônio foi realizado por meio de ultrassonografia transabdominal onde se observou a presença de imagens ecográficas sugestivas de alças intestinais flutuando em grande quantidade de líquido hipoecoico; e confirmado pela relação creatinina do líquido abdominal: creatininas é rica acima de dois. Primeiramente os potros foram submetidos à fluido terapia intravenosa associada à drenagem progressiva do líquido peritoneal para posterior celiotomia exploratória com o objetivo de localizar e reparar a lesão.Na potranca de 20 dias e no potro de 8 dias foi identificada uma ruptura de úraco. Na potranca de 12 dias, foi constatado um defeito congênito na parede dorsal da bexiga que foi reparado após leve debridamento dos bordos da lesão. Nos três casos foi efetuada a ressecção do úraco, veia e artérias umbilicais e posterior cistorrexia. Os animais de 20, 8 e 12 dias de idade apresentaram uma melhora rápida e sem complicações após a correção cirúrgica,recebendo alta aos nove, sete e 23 dias após a intervenção, respectivamente.(AU)

Three cases of uroperitoneum in Thorough bred foals, two females and one male, are described in the present study. The animais showed clinical sings of mild to moderate abdominal pain, tachycardia, tachypnea,abdominal distention and straining to urinate. The diagnosis of uroperitoneum was made by transabdominal ultrasound examination and confirmed by the ratio of [abdominal fluid creatinine] : [serum creatinine] greater than two. Initially the foals were treated with intravenous fluid therapy associated with progressive drainage of peritoneal fluid forsubsequent exploratory laparotomy in order to identify and repair the lesion. A ruptured urachus was identified in the20-day-old filly and in the 8-day-old colt. The 12-day-old filly had a congenital defect in the dorsal aspect of thebladder; this defect was repaired after debridement of the lesion edges. The resection of urachus, umbilical vein andarteries was pertormed in ali three cases followed by cistorrhaphy. The outcome was good without complications aftersurgical correction; the 20-day old filly , 8-day-old colt and the 12-day-old filly were discharged at 9,7 and 23 daysafter surgery, respectively.(AU)

Tres casos de uroperitoneo son descriptos, respectivamente, en dos potrancas y un potro Pura Sangrede Carrera de 12, 20 Y 8 días de edad. Los animales presentaban senales de dolor abdominal discreta a moderada,taquipnea, taquicardia, distensión abdominal y disuria. EI diagnóstico de uroperitoneo fue realizado por medio de ultrasonografía transabdominal, donde fueron observadas vueltas intestinales fluctuando en gran cantidad de líquido hipoecogénico; fue confirmado por Ia relación creatinina dei líquido abdominal: creatinina sé rica arriba de 2. Primeramente los productos fueron sometidos a Ia fluidoterápia intravenosa asociada a un drenaje progresivo deilíquido peritoneal para una posterior quirurgia abdominal exploratoria con el objetivo de localizar y reparar Ia lesión.En Ia potranca de 20 días y en el potro de 8 días fue identificada una ruptura de uraco. En Ia potranca de 12 días fue constatada una alteración congénita en Ia pared vesical dorsal que fue reparada después de un desbridamiento delos bordos de Ia lesión. En los 3 casos fue efectuada Ia resección dei uraco, de Ias venas y arterias umbilicales e unaposterior cistorrafia. Los 3 animales de 20, 8 Y 12 días de edad presentaron una rápida mejora sin complicaciones después de Ia corrección quirúrgica, y recibieron alta 9, 7 Y 23 días después.(AU)

Tensile strength of the posterior and anterior layer of the rectus abdominis muscle sheath in cadavers / Tensão das bainhas anterior e posterior do músculo reto do abdome em cadáveres

PURPOSE: The aim of the present study was to assess, in cadavers, the resistance to medial traction of the posterior and anterior layer of the rectus abdominis muscle sheath. METHODS: An anatomical, prospective, self-controlled study was carried out in 20 male cadavers. An evaluation of the tension of the posterior and anterior layers of the rectus abdominis muscle sheath was performed following anatomical dissection of these structures. The traction necessary to advance the tissue ten millimeters was assessed using an analogic dynamometer. RESULTS: There was no significant statistical difference between the necessary traction at various levels of the anterior and posterior layers of the rectus sheath. However, medial advancement of the anterior layer of the rectus sheath required more traction strength than did advancement of the posterior layer to the midline. CONCLUSION: There is no difference in the tensile strength in the various levels of the anterior and posterior layers of the rectus sheath; however, medial advancement of the the anterior layer required more traction strength.

OBJETIVO: Comparar a resistência à tração medial das lâminas posterior e anterior da bainha do músculo reto do abdome em diferentes níveis supra e infra-umbilicais em cadáveres. MÉTODOS: Vinte cadáveres adultos foram submetidos à dissecção da parede abdominal anterior. Foram feitas alças com fio de náilon no plano aponeurótico em oito pontos diferentes na lâmina anterior e na lâmina posterior da bainha do músculo reto do abdome, sendo quatro destes pontos supra-umbilicais e quatro infra-umbilicais. Com o uso de um dinamômetro analógico, foi verificada a força necessária para o avanço medial de dez milímetros destes dezesseis pontos. RESULTADOS: Não foram encontradas diferenças significantes de medidas da resistência à tração entre os diferentes níveis da aponeurose anterior e posterior, mas quando se comparou as medidas obtidas na aponeurose anterior às obtidas na posterior verificou-se que a aponeurose anterior necessitou de maior força de tração para o avanço medial. CONCLUSÃO: Não há diferença de tensão ao longo da aponeurose anterior e posterior. A aponeurose anterior é mais resistente à tração medial quando comparada à posterior.

Tensile strength of the posterior and anterior layer of the rectus abdominis muscle sheath in cadavers / Tensão das bainhas anterior e posterior do músculo reto do abdome em cadáveres

PURPOSE: The aim of the present study was to assess, in cadavers, the resistance to medial traction of the posterior and anterior layer of the rectus abdominis muscle sheath. METHODS: An anatomical, prospective, self-controlled study was carried out in 20 male cadavers. An evaluation of the tension of the posterior and anterior layers of the rectus abdominis muscle sheath was performed following anatomical dissection of these structures. The traction necessary to advance the tissue ten millimeters was assessed using an analogic dynamometer. RESULTS: There was no significant statistical difference between the necessary traction at various levels of the anterior and posterior layers of the rectus sheath. However, medial advancement of the anterior layer of the rectus sheath required more traction strength than did advancement of the posterior layer to the midline. CONCLUSION: There is no difference in the tensile strength in the various levels of the anterior and posterior layers of the rectus sheath; however, medial advancement of the the anterior layer required more traction strength.(AU)

OBJETIVO: Comparar a resistência à tração medial das lâminas posterior e anterior da bainha do músculo reto do abdome em diferentes níveis supra e infra-umbilicais em cadáveres. MÉTODOS: Vinte cadáveres adultos foram submetidos à dissecção da parede abdominal anterior. Foram feitas alças com fio de náilon no plano aponeurótico em oito pontos diferentes na lâmina anterior e na lâmina posterior da bainha do músculo reto do abdome, sendo quatro destes pontos supra-umbilicais e quatro infra-umbilicais. Com o uso de um dinamômetro analógico, foi verificada a força necessária para o avanço medial de dez milímetros destes dezesseis pontos. RESULTADOS: Não foram encontradas diferenças significantes de medidas da resistência à tração entre os diferentes níveis da aponeurose anterior e posterior, mas quando se comparou as medidas obtidas na aponeurose anterior às obtidas na posterior verificou-se que a aponeurose anterior necessitou de maior força de tração para o avanço medial. CONCLUSÃO: Não há diferença de tensão ao longo da aponeurose anterior e posterior. A aponeurose anterior é mais resistente à tração medial quando comparada à posterior.(AU)

Ultrasonography for measuring rectus abdominis muscles diastasis / Ultra-sonografia para medir a diástase dos músculos retos do abdome

PURPOSE: Preoperative imaging evaluation may be useful for determining the position of recti abdominis muscles before their correction. The purpose of this study is to evaluate the accuracy of ultrasonography to measure the width of rectus abdominis muscle diastasis. METHODS: Rectus diastasis was measured by ultrasonography preoperatively in 20 females. Rectus diastasis was measured in seven levels along the anterior rectus sheath by ultrasound after sustained deep inspiration and after expiration. Rectus diastasis, at the same levels, was also measured after its exposition during abdominoplasty by two independent observers, using a surgical compass. These values were compared using Wilcoxon's statistical text, for non independent values. RESULTS: There was no significant difference between the values obtained by ultrasound and those measured during surgery in the supra-umbilical levels and at the level of the umbilicus. However, below the umbilicus these values differed significantly, showing smaller values in the imaging evaluation. CONCLUSION: Ultrasonography is an accurate method to measure rectus diastasis above the umbilicus and at the umbilical level.(AU)

OBJETIVO: Estudos radiológicos pré-operatórios podem ser úteis para determinar a posição dos músculos retos do abdome antes de sua correção. O objetivo deste estudo é avaliar a acurácia da ultra-sonografia para medir a largura da diástase dos músculos retos do abdome. MÉTODOS: A diástase dos músculos retos foi medida pela ultra-sonografia, no pré-operatório em 20 mulheres. A diástase dos retos foi medida em sete níveis da bainha anterior dos retos foram examinados com ultra-som após inspiração profunda sustentada e após expiração. A diástase dos retos, nos mesmos níveis, foi também medida após sua exposição durante a abdominoplastia, por dois observadores independentes, usando compasso cirúrgico. As medidas foram comparadas usando o teste estatístico de Wilcoxon para dados não independentes. RESULTADOS: Não houve diferença significante entre as medidas ultra-sonográficas e intra-operatórias nos níveis supra-umbilicais e na cicatriz umbilical. Todavia, inferiormente à cicatriz umbilical, essas medidas diferiram significantemente sendo as ultra-sonográficas menores nestes pontos. CONCLUSÃO: A ultra-sonografia é um método acurado para medir a diástase de retos na região supra- umbilical e no nível do umbigo.(AU)

Experimental study comparing the tensile strength of different surgical meshes following aponeurotic-muscle deformity synthesis on Wistar rats / Estudo experimental comparando a resistência tênsil de diferentes tipos de telas cirúrgicas após síntese de defeito músculo-aponeurótico em ratos Wistar

PURPOSE: To assess the tensile strength of polypropylene and polypropylene associated with polyglactin meshes (Vypro II® - Ethicon®, Somerville, NJ, USA) in a situation of partial separation of abdominal muscle aponeurosis on rats. METHODS: Thirty rats were used of the Wistar strain, which were randomized into two groups of 15 specimens each. In both groups an aponeurotic-muscle deformity was created on the abdominal wall measuring 3.0 x 1.0 cm, which was closed with polypropylene mesh (polypropylene group) or Vypro® mesh (vypro group). After 28 days the rats underwent euthanasia and an area was removed from the abdominal wall with which a strip was made measuring 2.0 cm in length and 6.0 cm in width comprising the abdominal muscles with the implanted mesh. This sample was placed in a mechanical test machine in which a constant force was applied contrary to the tissue strips. Maximum force expressed in Newton was considered until full rupture of the sample occurred. The non-parametric Kruskal - Wallis test was used for statistical analysis admitting p<0.05. RESULTS: Out of the thirty animals, there were two deaths in the vypro group and one unit in the polypropylene group was lost. One animal in the polypropylene group developed hernia during the study and another one developed granuloma of the abdominal wall. All animals in both groups developed epiplon adherence to the mesh. The average force was 48.08 N for the polypropylene group and 45.32 for the vypro® group. CONCLUSION: In these experimental conditions it could be observed that there is no statistically significant difference in the rupture force of the polypropylene and Vypro® meshes (p=0.54).(AU)

OBJETIVO: Avaliar a resistência tênsil das telas de polipropileno e polipropileno associado à poliglactina (Vypro II® - Ethicon®, Somerville, NJ, USA) em situação de afastamento parcial da aponeurose dos músculos abdominais em ratos. MÉTODOS: Foram utilizados trinta ratos da linhagem Wistar randomizados em dois grupos de quinze exemplares cada um. Em ambos os grupos criou-se um defeito músculo-aponeurótico na parede abdominal medindo 3,0 x 1,0 cm que foi fechado com tela de polipropileno (grupo polipropileno) ou Vypro® (grupo vypro). Após 28 dias, foi feita a eutanásia e retirou-se uma área da parede abdominal com a qual fez-se uma tira medindo 2,0 cm de comprimento por 6,0 cm de largura englobando os músculos abdominais com a tela implantada. Essa amostra foi presa em máquina de ensaios mecânicos na qual se aplicou força constante contrária às tiras de tecido. Foi considerada a força máxima expressa em Newton até ocorrer a ruptura total da amostra. Para a análise estatística, utilizou-se teste não paramétrico de Kruskal - Wallis admitindo-se p<0,05. RESULTADOS: Ocorreram dois óbitos do grupo vypro e uma unidade do grupo polipropileno foi perdida no teste mecânico. Um animal do grupo polipropileno desenvolveu hérnia durante o período do estudo e outro desenvolveu um granuloma de parede abdominal. Todos os animais de ambos os grupos desenvolveram aderência de epíplon à tela. A média das forças foi de 48.08 N para o grupo polipropileno e 45.32 N para o grupo vypro®. CONCLUSÃO: A tela de polipropileno apresentou uma força média de resistência à tração maior quando comparada com a de Vypro® nessas condições experimentais.(AU)

Comparação entre a colopexia laparoscópica com pericárdio bovino conservado em glicerina em cães com a técnica incisional por celiotomia

The laparoscopic colopexy with bovine pericardium preserved in glycerol was compared to incisional approach by celiotomy. Twenty mongrel dogs were used after being separated in two groups. In the laparoscopic surgery, two incisions were made on the transverse abdominal muscles, and the implant was positioned through the muscle flap, involving the bowel. Finally, the extremities of the mesh were clipped. Twenty-eight days after this surgery, the experimental animals were submitted to a new laparoscopic surgery, now aiming at observing the results of both techniques. All patients of the celiotomy group maintained the colopexy. However, none of the animals submitted to the laparoscopic surgery maintained the bowel fixation. The proposed technique is not indicated to promote colopexy in dogs.

Procurou-se verificar a viabilidade da colopexia laparoscópica com o emprego de pericárdio bovino conservado em glicerina e testar seus resultados em relação ao procedimento incisional por celiotomia em 20 cães, separados em dois grupos com igual número de animais. A técnica laparoscópica envolveu a realização de duas incisões paralelas no músculo transverso abdominal, sob o qual foi posicionado um retalho da membrana conservada, que envolveu completamente o cólon descendente. Com a aplicação de dois clipes que uniram as bordas do pericárdio e com a sua fixação às camadas serosa e muscular do cólon, promoveu-se a aproximação do órgão à parede abdominal. Vinte e oito dias após, por ocasião do exame laparoscópico, verificou-se que todos os pacientes do grupo da celiotomia mantiveram a aderência do cólon, situação que não foi observada nos animais submetidos à cirurgia laparoscópica. Conclui-se que a técnica proposta não possibilita colopexia permanente, sendo contra-indicada para cães.

Comparação entre a colopexia laparoscópica com pericárdio bovino conservado em glicerina em cães com a técnica incisional por celiotomia / Comparative study between laparoscopic colopexy with bovine pericardium preserved in glycerol and incisional approach by celiotomy in dogs

Procurou-se verificar a viabilidade da colopexia laparoscópica com o emprego de pericárdio bovino conservado em glicerina e testar seus resultados em relação ao procedimento incisional por celiotomia em 20 cães, separados em dois grupos com igual número de animais. A técnica laparoscópica envolveu a realização de duas incisões paralelas no músculo transverso abdominal, sob o qual foi posicionado um retalho da membrana conservada, que envolveu completamente o cólon descendente. Com a aplicação de dois clipes que uniram as bordas do pericárdio e com a sua fixação às camadas serosa e muscular do cólon, promoveu-se a aproximação do órgão à parede abdominal. Vinte e oito dias após, por ocasião do exame laparoscópico, verificou-se que todos os pacientes do grupo da celiotomia mantiveram a aderência do cólon, situação que não foi observada nos animais submetidos à cirurgia laparoscópica. Conclui-se que a técnica proposta não possibilita colopexia permanente, sendo contra-indicada para cães(AU)

The laparoscopic colopexy with bovine pericardium preserved in glycerol was compared to incisional approach by celiotomy. Twenty mongrel dogs were used after being separated in two groups. In the laparoscopic surgery, two incisions were made on the transverse abdominal muscles, and the implant was positioned through the muscle flap, involving the bowel. Finally, the extremities of the mesh were clipped. Twenty-eight days after this surgery, the experimental animals were submitted to a new laparoscopic surgery, now aiming at observing the results of both techniques. All patients of the celiotomy group maintained the colopexy. However, none of the animals submitted to the laparoscopic surgery maintained the bowel fixation. The proposed technique is not indicated to promote colopexy in dogs(AU)

Effects of venous supercharging in deep inferior epigastric artery perforator flap / Efeitos da vascularização venosa ampliada na viabilidade do retalho perfurante da artéria epigástrica profunda

PURPOSE: To evaluate the effects of venous supercharging in deep inferior epigastric artery perforator flap in rats. METHODS: 20 Wistar rats were randomized in 2 groups: control group (C), all had flaps raised based on the deep inferior epigastric perforator vessels (DIEP), and experimental group (E), which was identical to group C, except that the contralateral superficial inferior epigastric vein was also kept with the flap. Flow studies using laser Doppler flowmetry where performed daily in the four zones of the flap. On the 7th postoperative day rats were killed and flap survival was determined using digital planimetry. RESULTS: Flow values were presented as a percentage of the baseline flow after incision of the flap edges. The surviving flap area was demonstrated as a percentage of the total flap area. Evaluation by digital planimetry showed that flap survival in group E was higher than in group C (97,38 percent±1,32 percentvs.44,13 percent±4,83 percent, p=0,0006). CONCLUSION: This study shows that venous supercharging of the rat DIEP flap results in greater flap survival(AU)

OBJETIVO: Avaliar o efeito da vascularização venosa ampliada na viabilidade do retalho perfurante da artéria epigástrica profunda em ratos. MÉTODOS: Vinte ratos foram divididos em 2 grupos: controle(C), no qual se realizou o retalho perfurante da artéria epigástrica profunda e experimento(E), no qual se realizou o mesmo retalho e se manteve a veia epigástrica superficial inferior contralateral ao pedículo. Foi realizada determinação diária do fluxo sanguíneo por fluxometria por laser-doppler em quatro quadrantes do retalho previamente estabelecidos e a viabilidade foi determinada, no 7º pós-operatório, através de planimetria. RESULTADOS: A análise do fluxo sangüíneo demonstrou não haver diferença entre o grupo C e E nas médias de fluxo entre as zonas do retalho (Zona I:103,44±8,09vs.84,70±7,98, p=0,114)(Zona II: 109,18±6,99 vs. 113,67±26,89, p=0,401)(Zona III: 89,15±11,11 vs. 106,79±15,93, p=0,599)(Zona IV: 104,43±11,50 vs. 124,90±23,17, p=1,00). A viabilidade do grupo E foi superior do que no grupo C quando determinada por planimetria digital (97,38 por cento±1,32 por cento vs. 44,13 por cento±4,83 por cento, p=0,0006). CONCLUSÃO: Há aumento da viabilidade do retalho DIEP submetidos à supercharging(AU)