Resumo

Chrozophora tinctoria (L.) A.Juss. is herbaceous, monecious annual plant used traditionally to cure gastrointestinal disorders. The present study was carried out to find the bioactive compounds by Gas Chromatography-Mass Spectrometry, the acetylcholinesterase inhibitory potential acute toxicity, and emetic activity present in the ethyl acetate fraction of Chrozophora tinctoria (EAFCT) and dichloromethane fraction of Chrozophora tinctoria (DCMFCT). The compounds detected in both fractions were mostly fatty acids, with about seven compounds in EAFCT and 10 in DCMFCT. These included pharmacologically active compounds such as imipramine, used to treat depression, or hexadecanoic acid methyl ester, an antioxidant, nematicide, pesticide, hypocholesterolemic, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- is used as a cancer preventive, antiarthritic, antihistaminic, hepatoprotective, insectifuge, nematicide, Pentadecanoic acid, 14-methyl-, methyl ester have antifungal, antimicrobial and antioxidant activities, 10-Octadecanoic acid, methyl ester have the property to decrease blood cholesterol, Antioxidant and antimicrobial, 1-Eicosanol is used as an antibacterial, 1-Hexadecene has antibacterial, antioxidant, and antifungal activities. Both DCMFCT and EAFCT fractions inhibited acetylcholinesterase (AChE) activity with IC50 values of 10 µg and 130 µg, respectively. Both the fractions were found to be toxic in a dose-dependent manner, inducing emesis at 0.5g onward and lethargy and mortality from 3­5 g upwards. Both the fractions combined with distilled water showed highly emetic activity. The significant increase in the number of vomits was shown by EAFCT plus distilled water which are 7.50±1.29, 7.25±3.10, and 11.75±2.22 number of vomits at 1g, 2g, and 3g/kg concentration respectively, while DCMFCT plus distilled water showed 5.25±2.22, 7.50±2.52 and 10.25±2.22 number of vomits at 1g, 2, and 3g/kg correspondingly. The antiemetic standard drug metoclopramide has a higher impact against the emesis induced by both the fractions than dimenhydrinate. Metoclopramide decreases the number of vomits caused by EAFCT to 1.00±0.00, 2.00±0.00, 4.00±1.00 at 1g, 2, and 3g/kg sequentially, while dimenhydrinate decreases the number of vomits to 1.33±0.58, 2.33±1.15, 4.33±0.58 at 1g, 2, and 3g respectively. In the same way, Metochloprimide decreases the number of emesis caused by DcmCt from 5.25±2.22, 7.50±2.52, 10.25±2.22 to 1.33±0.58, 2.33±1.1, 4.33±0.58 at 1g, 2, and 3g/kg concentrations. The present study is the first documented report that scientifically validates the folkloric use of Chrozophora tinctoria as an emetic agent.

Chrozophora tinctoria (L.) A.Juss. é uma planta anual herbácea, monoica, usada tradicionalmente para curar distúrbios gastrointestinais. O presente estudo foi realizado para encontrar os compostos bioativos por Cromatografia Gasosa-Espectrometria de Massa (GC-MS), a toxicidade aguda do potencial inibitório da acetilcolinesterase e a atividade emética presente na fração acetato de etila de Chrozophora tinctoria (EAFCT) e fração diclorometano de Chrozophora tinctoria (DCMFCT). Os compostos detectados em ambas as frações foram principalmente ácidos graxos, com cerca de sete compostos em EAFCT e 10 em DCMFCT. Estes incluíam compostos farmacologicamente ativos, como a imipramina, usada para tratar a depressão, ou éster metílico do ácido hexadecanoico, um antioxidante, nematicida, pesticida, hipocolesterolêmico, ácido 9,12,15-octadecatrienoico, éster etílico, (Z,Z,Z)- é usado como preventivo do câncer, antiartrítico, anti-histamínico, hepatoprotetor, inseticida, nematicida, ácido pentadecanoico, 14-metil-, éster metílico tem atividades antifúngicas, antimicrobianas e antioxidantes, ácido 10-octadecanoico, éster metílico tem a propriedade de diminuir o colesterol no sangue, antioxidante e antimicrobiano, o 1-Eicosanol é usado como antibacteriano, o 1-Hexadeceno possui atividades antibacteriana, antioxidante e antifúngica. Ambas as frações DCMFCT e EAFCT inibiram a atividade da acetilcolinesterase (AChE) com valores de IC50 de 10µg e 130µg, respectivamente. Ambas as frações foram consideradas tóxicas de maneira dose-dependente, induzindo vômitos a partir de 0,5g e letargia e mortalidade de 3g a 5g para cima. Ambas as frações combinadas com água destilada apresentaram atividade altamente emética. O aumento significativo no número de vômitos foi demonstrado pelo EAFCT mais água destilada, que são 7,50±1,29, 7,25±3,10 e 11,75±2,22 número de vômitos nas concentrações de 1g, 2g e 3g/kg, respectivamente, enquanto DCMFCT mais água destilada mostrou 5,25±2,22, 7,50±2,52 e 10,25±2,22 número de vômitos em 1g, 2g e 3g/kg, respectivamente. A droga padrão antiemética metoclopramida tem um impacto maior contra a emese induzida por ambas as frações do que o dimenidrinato. A metoclopramida diminui o número de vômitos causados ​​por EAFCT para 1,00±0,00, 2,00±0,00, 4,00±1,00 a 1g, 2g e 3g/kg, sequencialmente, enquanto o dimenidrinato diminui o número de vômitos para 1,33±0,58, 2,33±1,15, 4,33± 0,58 a 1g, 2g e 3g, respectivamente. Da mesma forma, a metocloprimida diminui o número de vômitos causados ​​por DcmCt de 5,25±2,22, 7,50±2,52, 10,25±2,22 para 1,33±0,58, 2,33±1,1, 4,33±0,58 nas concentrações de 1g, 2g e 3g/kg. O presente estudo é o primeiro relato documentado que valida cientificamente o uso folclórico de Chrozophora tinctoria como agente emético.

Resumo

The effects of the Oleoresin Capsicum (OC) spray used by police forces to control civil disturbances are widely known and extensively described in numerous scientific articles regarding humans. However, with the advent of animal welfare concepts, the question arises as to whether horses exposed to OC in such events would experience the same effects described in humans. The objective of this study was to determine if Mounted Police horses exposed to OC exhibit the same symptoms described in humans. In this study, 18 healthy horses from the Military Police of Paraná, without a history of respiratory disease, were led through a cloud of gas created using six pepper spray dispensers. Physiological parameters including respiratory rate, heart rate, rectal temperature, and conjunctival color were evaluated 24 hours before exposure, 30 minutes, and 24 hours after exposure. Blood samples were collected for a complete blood count and blood gas analysis, and samples of the ocular mucosa were obtained using sterile swabs. Analysis of these parameters did not reveal effects analogous to those described in humans. The absence of ocular changes is directly correlated with the primary decontamination method in humans, which involves running with open eyes to allow the air to act against them. In the case of horses, the movement of their eyes while walking at an average speed of 6km/h in a walk and 18km/h in a trot generates airflow throughout the entire body of the animal. Additionally, pieces of tack and parts of the rider's body form a barrier against the action of the OC agent by reducing the contact area. After 30 minutes and 24 hours post-exposure, no aggressive effects like those found in humans in the airways and ocular mucosa were observed.

Os efeitos sobre seres humanos do spray Oleoresin Capsicum (OC) utilizado pelas forças policiais no controle de distúrbios civis são amplamente conhecidos e largamente descritos em numerosos artigos científicos. No entanto, com o advento dos conceitos de bem-estar animal, levanta-se a questão se equinos expostos ao OC em tais eventos sofreriam os mesmos efeitos descritos em seres humanos. O objetivo deste estudo foi determinar se cavalos da Polícia Montada expostos ao OC apresentam os mesmos sintomas descritos em seres humanos. Neste estudo, 18 cavalos da Polícia Militar do Paraná, saudáveis e sem histórico de doença do trato respiratório, foram conduzidos por uma nuvem de gás criada a partir do uso de seis espargidores de spray pimenta. Os parâmetros fisiológicos de frequência respiratória, frequência cardíaca, temperatura retal e coloração das mucosas foram avaliados 24 horas antes da exposição, 30 min e 24 horas após a exposição. Amostras de sangue foram coletadas para realização de hemograma completo e hemogasometria, e amostras da mucosa ocular foram obtidas por meio de swabs estéreis. A análise desses parâmetros não revelou efeitos análogos aos descritos em seres humanos. A não manifestação de alterações oculares está diretamente correlacionada com a forma primária de descontaminação em seres humanos, que é correr com os olhos abertos a fim de permitir a ação do do ar contra os olhos. No caso dos equinos, o deslocamento deles, no andamento ao passo, em uma velocidade média de 6km/h, e ao trote, em uma velociade média de 18km/h, gera um fluxo de ar em todas as partes do corpo do animal; além disso, peças de selaria e partes do corpo do cavaleiro formam uma barreira contra a ação do agente OC, diminuindo, dessa forma, a área de contato. Após 30 minutos e 24 horas depois da exposição, também não foram observados efeitos agressores análogos aos encontrados nas vias áerea e conjuntivas oculares em seres humanos.

Resumo

This study compared cardiovascular and respiratory effects of dexmedetomidine and xylazine in total intravenous anesthesia with propofol, ketamine, and lidocaine. Twenty-one female dogs were submitted to ovariohysterectomy, premedicated with acepromazine and anesthetized with propofol at a variable rate. The dogs were intubated and supplemented with 100% oxygen in a circuit without rebreathing gases in spontaneous ventilation. They were divided into three groups (n=21) after induction: control (CON) with ketamine (2 mg/kg + 0.6 mg/kg/h) and lidocaine (2 mg/kg + 3 mg/kg/h), DEX and XIL with the same drugs as CON, associated with dexmedetomidine (2 µg/kg + 1 µg/kg/h) or xylazine (0.2 mg/kg + 0.1 mg/kg/h). Propofol consumption, fentanyl analgesic rescue, and cardiorespiratory and blood gas parameters were evaluated during anesthesia. The DEX group had a lower consumption of propofol (0.16 ± 0.09 mg/kg/min) compared to CON (0.24 ± 0.09 mg/kg/min), both not differing from XIL (0.23 ± 0.09 mg/kg/min). The mean arterial pressure was higher after the initial bolus in DEX (107 ± 8 mmHg) and XIL (96 ± 11 mmHg) compared to the CON group (80 ± 10 mmHg). Higher accumulation of arterial carbon dioxide and a decrease in pH were observed in the CON group. The total number of fentanyl rescues did not differ between DEX (7) and XIL (6) and were lower than CON (16). Therefore, dexmedetomidine and xylazine reduced intraoperative fentanyl consumption compared to ketamine and lidocaine infusion alone. However, only dexmedetomidine promoted lower propofol consumption and higher blood pressure values.

Esse estudo comparou efeitos cardiovasculares e respiratórios da dexmedetomidina e xilazina na anestesia total intravenosa com propofol, cetamina e lidocaína. Foram submetidas a ovário-histerectomia 21 cadelas, pré-medicadas com acepromazina, anestesiadas com propofol em taxa variável. As cadelas foram intubadas e suplementadas com oxigênio 100% em circuito sem reinalação de gases em ventilação espontânea. Após a indução, foram distribuídas em três grupos (n=21): controle (CON) com cetamina (2 mg/kg + 0,6 mg/kg/h) e lidocaína (2 mg/kg + 3 mg/kg/h), DEX e XIL com os mesmos fármacos do CON, associados à dexmedetomidina (2 µg/kg + 1 µg/kg/h) ou xilazina (0,2 mg/kg + 0,1 mg/kg/h). Durante a anestesia, foram avaliados o consumo de propofol, resgate analgésico de fentanil, parâmetros cardiorepiratórios e hemogasométricos. O grupo DEX apresentou menor consumo de propofol (0,16 ± 0,09 mg/kg/min) comparado ao CON (0,24 ± 0,09 mg/kg/min), ambos sem diferir de XIL (0,23 ± 0,09 mg/kg/min). A pressão arterial média foi maior após o bolus inicial em DEX (107 ± 8 mmHg) e XIL (96 ± 11 mmHg) comparadas ao grupo CON (80 ± 10 mmHg). Houve maior acúmulo de gás carbônico arterial e diminuição do pH no grupo CON. O número total de resgates com fentanil não diferiram entre DEX (7) e XIL (6), e foram inferiores ao CON (16). Conclui-se que a dexmedetomidina e xilazina reduziram o consumo de fentanil transoperatório em comparação à infusão isolada de cetamina e lidocaína. Porém, nesse estudo apenas a dexmedetomidina promoveu menor consumo de propofol e maiores valores de pressão arterial.

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The papaya (Carica papaya L.) is among the mainly fruit species produced in tropical and subtropical climate. The salinity of water in agricultural areas is considered a limiting factor for the expansion of papaya. This study aimed to evaluate calcium-enriched microalgae extract (EMa-Ca) as an attenuator of saline stress in irrigation water on the growth and physiology of Formosa papaya seedlings, hybrid Tainung. The experiment was conducted in a protected environment, with treatments distributed in a 5 × 2 factorial scheme, comprising five electrical conductivities of irrigation water (0.50; 1.10; 2.50; 3.90 and 4.50 dSm-1) with the presence and absence of EMa-Ca in the substrate. Evaluated characteristics were: plant height, number of leaves, stem diameter, leaf area, dry masses weight of roots, aboveground parts and total. Gas exchanges and chlorophyll indices (a, b and total) were also evaluated. The application of EMa-Ca resulted in an increase of 6.05% in height and 6.33% in trunk diameter. The number of leaves decreased with an increase in electrical conductivity, and the leaf area was reduced by 33%. All seedling dry masses showed greater declines in the absence of EM-Ca. The EMa-Ca increased net photosynthesis, CO2 concentration, transpiration and stomatal conductance by 39.13%, 30.43%, 38.88% and 42.85%, respectively. For chlorophyll without the use of EMa-Ca, a decrease rate of 1.21%, 0.41% and 1.62% was observed for Chla, Chlb and Chlt, respectively. Therefore, the EMa-Ca application (1.0 ml/L) significantly enhance the vegetative development, gas exchanges, and chlorophyll indices of papaya seedlings under saline stress conditions.

O mamão (Carica papaya L.) está entre as principais espécies frutíferas produzidas em clima tropical e subtropical. A condição de salinidade da água nas áreas agrícolas pode ser considerada um fator limitante para a expansão do mamão. Este estudo teve como objetivo avaliar o extrato de microalga enriquecido com cálcio (EMa-Ca) como atenuador do estresse salino em água de irrigação no crescimento e na fisiologia de mudas de mamão Formosa, híbrido Tainung. O experimento foi conduzido em ambiente protegido, com tratamentos distribuídos em esquema fatorial 5 × 2, compreendendo cinco condutividades elétricas da água de irrigação (0,50; 1,10; 2,50; 3,90 e 4,50 dSm-1) com a presença e ausência no substrato de EMa-Ca. As características avaliadas foram: altura da planta, número de folhas, diâmetro do caule, área foliar, peso de massa seca das raízes, partes aérea e total. Também foram avaliadas as trocas gasosas e os índices de clorofila (a, b e total). A aplicação de EMa-Ca resultou em aumento de 6,05% na altura e 6,33% no diâmetro do tronco. O número de folhas diminuiu com aumento da condutividade elétrica e a área foliar reduziu 33%. Todas as massas secas das mudas apresentaram maiores declínios na ausência do EM-Ca. O EMa-Ca aumentou a fotossíntese líquida, concentração de CO2, transpiração e condutância estomática em 39,13%, 30,43%, 38,88% e 42,85%, respectivamente. Para a clorofila sem a utilização do EMa-Ca observa-se uma taxa de diminuição de 1,21%, 0,41% e 1,62% para Chla, Chlb e Chlt, respectivamente. Portanto, a aplicação de EMa-Ca (1,0 ml/L) melhora significativamente o desenvolvimento vegetativo, as trocas gasosas e os índices de clorofila de mudas de mamoeiro sob condições de estresse salino.

Resumo

This study was carried out to evaluate the effects of corn dried distillers' grains (DDG) levels in non-forage diets by in vitro, in situ (0 to 50%), and in vivo (0 to 40%) trials on kinetic parameters of gas production (GP) on rumen-degradable protein (RDP) and rumen-undegradable protein (RUP) as well as on nitrogen (N) metabolism in Nellore cattle. For in vitro and in situ studies, three rumen cannulated Nellore males with body weight of 340.48 ± 22.22 kg were used. For the in vivo study, five non-castrated male Nellore cattle with an initial body weight of 355.20 ± 35.28 kg and 24 ± 3 months old were used to evaluate the effect of increasing DDG levels in non-forage diets on N metabolism by a 5 × 5 Latin square design. Experimental diets were based on ground corn, cottonseed cake, urea, mineral supplement, and increasing levels of DDG replacing ground corn. The DDG levels caused a linear decrease in GP and in vitro dry matter digestibility. The DDG presented a lower dry matter digestibility (DMD) and RDP and higher RUP than corn grain and cottonseed cake, making DDG diets present lower DMD and RDP than control. The DDG levels linearly increased the intake of crude protein, neutral detergent fiber, and ether extract, whereas non-fiber carbohydrates intake decreased and tended to decrease the DMD and organic matter digestibility. The DDG levels caused a linear increase in N intake and in total, fecal, and urinary N excretion, which was the major N excretion route, causing a quadratic drop in retained N and caused a quadratic effect on alanine aminotransferase. In contrast, triglycerides were cubically affected, and total blood protein increased. Thus, DDG levels negatively affect in vitro and in situ digestibility, increasing N intake but increasing urinary N excretion.(AU)

Resumo

Fine-fleeced sheep are distinguished by numerous economically valuable traits that constitute the foundation for productive distinctions among breeds, populations, lines, and individuals. Many of these traits have already been mentioned or thoroughly examined during studies on the correlative variability of productivity indicators, blood parameters, characteristics of pulmonary gas exchange, histological structures of the skin, and features of the experimental sheep's coat. The objective of our research was to investigate the correlative variability of key economically valuable traits that characterize the overall functional state of sheep organisms under varying environmental conditions. The study was conducted at the "Sharbulak" breeding farms and the "Samat" peasant farms in the Kazygurt district of the Turkestan region. Our findings reveal that one-year-old rams surpass ewes in terms of live weight by 32-37% and in terms of unwashed wool shearing by 21-23%. Two-year-old rams outperform ewes in live weight by 2.15-2.17 times and in unwashed wool shearing by 2.38-2.44 times. The highest phenotypic variability in relative terms (as indicated by the coefficient of variation) is observed in the shearing of pure wool, with an average coefficient of variation of 18.1% across all sex and age groups of sheep. This is followed by the wool coefficient (17.4%) and the yield of pure fiber (12.5%). For each group of animals, the most substantial phenotypic correlation coefficients were observed between the live weight of sheep and the shearing of unwashed wool. On average, across all groups of sheep at the "Sharbulak" breeding farm, this phenotypic correlation reaches +0.411 ± 0.077. Correspondingly, for the sheep herd at the "Samat" peasant farm, it is +0.326 ± 0.075. The second-highest phenotypic correlation pertains to the cut of unwashed wool and the length of wool (with correlation coefficients of +0.156 ± 0.058 and +0.145 ± 0.057, respectively, for the herds). The third-highest correlation involves live weight and wool length (+0.131 ± 0.085 and +0.105 ± 0.078, respectively). No statistically significant differences were identified in the average correlation coefficients between the live weight of sheep, the shearing of unwashed wool, and the length of the staple among the flocks of sheep at the "Sharbulak" breeding farm and the "Samat" peasant farm.

Ovelhas de lã fina distinguem-se por numerosas características economicamente valiosas que constituem a base para distinções produtivas entre raças, populações, linhagens e indivíduos. Muitas dessas características já foram mencionadas ou examinadas minuciosamente durante estudos sobre a variabilidade correlativa de indicadores de produtividade, parâmetros sanguíneos, características das trocas gasosas pulmonares, estruturas histológicas da pele e características da pelagem experimental da ovelha. O objetivo da nossa pesquisa foi investigar a variabilidade correlativa das principais características economicamente valiosas que caracterizam o estado funcional geral dos organismos ovinos sob diversas condições ambientais. O estudo foi realizado nas fazendas de criação "Sharbulak" e nas fazendas camponesas "Samat" no distrito de Kazygurt, na região do Turquestão. Nossas descobertas revelam que os carneiros com um ano de idade superam as ovelhas em termos de peso vivo em 32-37%, e em termos de tosquia de lã não lavada, em 21-23%. Carneiros de dois anos superam as ovelhas em peso vivo em 2,15-2,17 vezes, e em tosquia de lã não lavada, em 2,38-2,44 vezes. A maior variabilidade fenotípica em termos relativos (conforme indicado pelo coeficiente de variação) é observada na tosquia de lã pura, com um coeficiente de variação médio de 18,1% em todos os sexos e faixas etárias das ovelhas. Seguem-se o coeficiente de lã (17,4%) e o rendimento de fibra pura (12,5%). Para cada grupo de animais, foram observados os coeficientes de correlação fenotípica mais substanciais entre o peso vivo dos ovinos e a tosquia da lã não lavada. Em média, em todos os grupos de ovinos da exploração de criação "Sharbulak", esta correlação fenotípica atinge +0,411 ± 0,077. Correspondentemente, para o rebanho ovino da fazenda camponesa "Samat", é de +0,326 ± 0,075. A segunda maior correlação fenotípica refere-se ao corte da lã não lavada e ao comprimento da lã (com coeficientes de correlação de +0,156 ± 0,058 e +0,145 ± 0,057, respectivamente, para os rebanhos). A terceira maior correlação envolve peso vivo e comprimento da lã (+0,131 ± 0,085 e +0,105 ± 0,078, respectivamente). Não foram identificadas diferenças estatisticamente significativas nos coeficientes de correlação médios entre o peso vivo dos ovinos, a tosquia da lã não lavada e o comprimento do alimento básico entre os rebanhos de ovinos da quinta de criação "Sharbulak" e da quinta camponesa "Samat".

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It aimed to compare the trans anesthetic clinical and perioperative analgesic effects by prolonged continuous infusion of fentanyl (GF, bolus of 5 µg/kg and continuous infusion (CI) of 15 µg/kg/h) or remifentanil (GR, CI of 18 µg/kg/h) in dogs undergoing to elective ovariohysterectomy. Twenty female healthy dogs were premedicated with acepromazine, inducted to unconsciousness with propofol and kept under anesthesia with isoflurane. The M0 (baseline) was recorded and animals were randomly allocated in GF (n = 10) or GR (n = 10), recording clinical and blood gas parameters every 15 minutes (M15…M120) for two hours and, after, during surgery, adjusting the opioid rate according to surgical requirement. The isoflurane vaporization reduced by up to 47% and 42% in GF and GR, respectively, when compared to M0. During the first two hours of CI, six animals of each group required atropine intervention and three animals of GF required ephedrine. The number of rate adjustments during intraoperative was significant higher in GR when compared to GF (P = 0.0248). By the Log-rank test, there was a higher possibility of not receiving analgesic rescue in the first 30 minutes of postoperative in GF when compared to GR (P < 0.0001) and all animals required analgesic rescue during the firsts 3 and 6 hours in GR and GF, respectively. It is concluded that, when the proposal continuous infusions were compared, fentanyl required fewer analgesic rescues during perioperative, nonetheless, it is recommended rate adjustment during intraoperative and additional analgesia for postoperative for both treatments.

Objetivou-se comparar os efeitos clínicos intraoperatórios e analgésico perioperatório ofertados pela infusão contínua prolongada de fentanil (GF, bolus de 5 µg/kg e infusão contínua (IC) de 15 µg/kg/h) ou remifentanil (GR, IC de 18 µg/kg/h) em cadelas submetidas a ovariohisterectomia eletiva. Vinte cadelas hígidas foram pré-medicadas com acepromazina e induzidas com propofol e mantidas sob anestesia com isoflurano. Registrado M0 (basal) os animais foram alocados aleatoriamente em GF (n = 10) ou GR (n = 10), registrando parâmetros clínicos e hemogasométricos a cada 15 minutos (M15...M120), totalizando 02h e, posteriormente, durante o procedimento cirúrgico, sendo a taxa do opioide alterada conforme o requerimento cirúrgico.A vaporização de isoflurano reduziu em até 47% e 42% no GF e GR, respectivamente, em relação ao M0. Durante as primeiras duas horas de IC, seis animais de cada grupo necessitaram de intervenção com atropina e três animais do GF de efedrina. O número de ajustes de taxas no intraoperatório foi significativamente maior no GR comparado ao GF (P = 0,0248). Pelo teste de Log-rank, houve maior probabilidade de não receber resgate analgésico nos primeiros 30 minutos de pós-operatório no GF comparado ao GR (P < 0,0001) e todos os animais necessitaram de resgate analgésico dentro das primeiras três e seis horas no GR e GF, respectivamente. Conclui-se que, nas taxas propostas, a utilização de fentanil necessitou de menos resgates analgésicos no intra e pós-operatório, no entanto, recomenda-se ajuste de taxa intraoperatória e analgesia suplementar pós-operatória em ambos os tratamentos.

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This study determines the associations among serum lipid profiles, risk of cardiovascular disease, and persistent organic pollutants. Using Gas chromatography technique, the intensity of toxic pollutant residues in serum samples of Hypertensive patients were measured. Based on statistical analysis, the effects of different covariates namely pesticides, age, systolic blood pressure, diastolic blood pressure, and lipid profile duration was checked using the logistic regression model. Statistical computation was performed on SPSS 22.0. The P-values of F-Statistic for each lipid profile class are greater than 0.01 (1%), therefore we cannot reject the null hypothesis for all cases. The estimated coefficients, their standard errors, Wald Statistic, and odds ratio of the binary logistic regression model for different lipid profile parameters indicate if pesticides increase then the logit value of different lipid profile parameters changes from -0.46 to -0.246 except LDL which increases by 0.135. The study reports a significantly increased threat of cardiovascular disease with increased concentrations of toxic pollutants.

Este estudo determina as associações entre o perfil lipídico sérico, o risco de doença cardiovascular e os poluentes orgânicos persistentes. Por meio da técnica de cromatografia gasosa, mediu-se a intensidade dos resíduos de poluentes tóxicos em amostras de soro de pacientes hipertensos. Com base na análise estatística, os efeitos de diferentes covariáveis – ou seja, pesticidas, idade, pressão arterial sistólica, pressão arterial diastólica e duração do perfil lipídico – foram verificados usando o modelo de regressão logística. O cálculo estatístico foi realizado no SPSS 22.0. Os valores P da estatística F para cada classe de perfil lipídico são maiores que 0,01 (1%), portanto não podemos rejeitar a hipótese nula para todos os casos. Os coeficientes estimados, seus erros padrão, estatística de Wald e odds ratio do modelo de regressão logística binária para diferentes parâmetros do perfil lipídico indicam se os pesticidas aumentam, então o valor logit de diferentes parâmetros do perfil lipídico muda de -0,46 para -0,246, exceto LDL, que aumenta em 0,135. O estudo relata um aumento significativo da ameaça de doença cardiovascular com aumento das concentrações de poluentes tóxicos.

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Background: Pyometra is a bacterial and hormone-induced reproductive disease that occurs in the post-estrus luteal phase in intact queens. Pyometra is more common in the diestrus period due to the high progesterone concentration (in queens that mated, spontaneously ovulated, or were induced to ovulate). However, it can also be seen due to the use of exogenous hormones such as progesterone for the suppression of estrus. More research is needed in cases of pyometra in queens, as well as in bitches. Because, considering that the pathogenesis and characteristics of feline pyometra is similar to bitches, studies on pyometra-affected bitches are taken as reference in studies and applications on queens. From this point of view, the aims of this study were to reveal the changes in complete blood count, blood gas, and serum biochemistry parameters in feline pyometra cases and to determine the correlation between the mentioned parameters. Materials, Methods & Results: In the study, a total of 25 female cats of different breeds were used, between the ages of 6 months and 7 years, 15 were diagnosed with pyometra, and 10 healthy. Anamnesis, clinical findings, and ultrasonographic examinations were used in the diagnosis of pyometra. Abdominal ultrasonography was performed on queens brought to the clinic with complaints such as anorexia, polydipsia, polyuria, abdominal tension, and fever. The control group (n =10) consisted of queens that were introduced to the clinic and were reproductively healthy. Before any treatment in queens with pyometra and the control group, 1 mL blood samples were taken from v. cephalica to evaluate complete blood count, blood gases and serum biochemistry parameters. In complete WBC, Lym, Mon, Gra, RBC, Hb, HCT, MCV, MCH and PLT parameters and, blood gas parameters such as pH, pCO2 , pO2 , sO2 , Na, K, Cl, lactate, glucose, HCO3 , and BE were also evaluated in taken blood samples. Biochemical parameters BUN, creatinine, ALT, AST, ALP, amylase, T.BIL, D.BIL, P, CHOL, TG, LDH, TP, CPK, ALP, Ca, GGT were measured in serum samples. After examination and laboratory analysis, ovariohysterectomy was performed on queens as a treatment. Granulocyte, WBC, HCT and MCH levels of the pyometra group were higher (P < 0.05) and Lym levels were lower (P < 0.05) compared to the control group. According to these results, pH, HCO3 , and BE were lower (P < 0.05) in queens with pyometra than those in the control group, while Na and lactate parameters were higher (P < 0.05). According to the results of biochemical analysis, it was determined that BUN, creatinine levels, GGT, and LDH enzyme activities were found to be higher in the pyometra group compared to the control group, while the Ca level was found to be low (P < 0.05). A positive correlation was observed between BUN and creatinine and LDH, WBC, granulocyte, HCT, and lactate, and a negative correlation between lymphocytes, pH, and BE in the correlation analysis performed on queens with pyometra and control group. However, a positive correlation was observed between creatinine and LDH and HCT, and a negative correlation between lymphocyte, pH and BE. Discussion: There is not enough information about pyometra in queens. As a result, it was determined that there were significant changes in complete blood count, blood gases and serum biochemical parameters in queens with pyometra in this study. These changes were generally thought to be related to dehydration and sepsis or endotoxemia. In addition, it was evaluated that prerenal azotemia occurring in pyometra affected queens may cause renal dysfunction. For this reason, it is thought that the results obtained in the presented study may contribute to the diagnosis, treatment, and prognosis of pyometra cases in queens.

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Abstract This study determines the associations among serum lipid profiles, risk of cardiovascular disease, and persistent organic pollutants. Using Gas chromatography technique, the intensity of toxic pollutant residues in serum samples of Hypertensive patients were measured. Based on statistical analysis, the effects of different covariates namely pesticides, age, systolic blood pressure, diastolic blood pressure, and lipid profile duration was checked using the logistic regression model. Statistical computation was performed on SPSS 22.0. The P-values of F-Statistic for each lipid profile class are greater than 0.01 (1%), therefore we cannot reject the null hypothesis for all cases. The estimated coefficients, their standard errors, Wald Statistic, and odds ratio of the binary logistic regression model for different lipid profile parameters indicate if pesticides increase then the logit value of different lipid profile parameters changes from -0.46 to -0.246 except LDL which increases by 0.135. The study reports a significantly increased threat of cardiovascular disease with increased concentrations of toxic pollutants.

Resumo Este estudo determina as associações entre o perfil lipídico sérico, o risco de doença cardiovascular e os poluentes orgânicos persistentes. Por meio da técnica de cromatografia gasosa, mediu-se a intensidade dos resíduos de poluentes tóxicos em amostras de soro de pacientes hipertensos. Com base na análise estatística, os efeitos de diferentes covariáveis ou seja, pesticidas, idade, pressão arterial sistólica, pressão arterial diastólica e duração do perfil lipídico foram verificados usando o modelo de regressão logística. O cálculo estatístico foi realizado no SPSS 22.0. Os valores P da estatística F para cada classe de perfil lipídico são maiores que 0,01 (1%), portanto não podemos rejeitar a hipótese nula para todos os casos. Os coeficientes estimados, seus erros padrão, estatística de Wald e odds ratio do modelo de regressão logística binária para diferentes parâmetros do perfil lipídico indicam se os pesticidas aumentam, então o valor logit de diferentes parâmetros do perfil lipídico muda de -0,46 para -0,246, exceto LDL, que aumenta em 0,135. O estudo relata um aumento significativo da ameaça de doença cardiovascular com aumento das concentrações de poluentes tóxicos.

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ABSTRACT: In some situations, the neonatal mortality rate in dogs can be high, and perinatal and postnatal veterinary care is essential to improve survival. This study to compared the Apgar score, serum lactate levels, and blood gas analysis results in 30 neonates born by elective cesarean section (GCE, n = 18) or cesarean section due to dystocia (GD, n = 12). Neonates were assessed at five time points: T0, at birth; T1, 3 h after birth; T2, 6 h after birth; T3, 12 h after birth; and T4, 24 h after birth. At T0, in the GCE group, 55.6% of the animals presented with adequate vitality, while 38.8% showed moderate changes in the Apgar score. In the GD group, 83.3% of the neonates had severe loss of vitality. At T1, none of the GCE neonates and 8.3% of the GD neonates presented with vitality deficits. Hyperlactatemia was observed in 83.3% of the GD neonates, and mixed acidosis (metabolic and respiratory) was observed at T0 in most neonates in both groups (GCE, 55.6%; GD, 72.7%). Compared to GCE neonates, the acid-base disorders were more severe and their recovery slower in GD neonates. At T0, GD neonates had higher hyperlactatemia and a lower Apgar score (worse vitality) than those of GCE neonates.

RESUMO: A taxa de mortalidade neonatal em cães pode ser elevada em algumas situações e a assistência veterinária peri e pós-natal mostra-se essencial para melhorar a sobrevivência dos filhotes nesse período. O objetivo deste estudo foi comparar o escore Apgar, lactato sérico e hemogasometria em neonatos nascidos por cesariana eletiva (GCE, n=18) e por cesariana após distocia (GD, n=12). Os neonatos foram avaliados em cinco momentos: (T0) ao nascimento; (T1) três horas; (T2) seis horas; (T3) 12 horas; e (T4) 24 horas. Ao nascimento, no GCE, 55,6% dos animais apresentaram boa vitalidade, de acordo com escore Apgar, e 38,8% demonstraram moderada vitalidade. No GD, 83,3% dos neonatos apresentaram baixa vitalidade. Ao T1 (três horas após o nascimento), nenhum (0%) dos neonatos do GCE e 8,3% dos neonatos do GD evidenciavam perda de vitalidade. Observou-se hiperlactatemia em 83,3% nos neonatos do GD e acidose mista (metabólica e respiratória) ao nascimento na maioria dos neonatos de ambos os grupos (55,6% no GCE e 72,7% no GD). Conclui-se que o principal distúrbio ácido-base observado ao nascimento foi acidose mista (metabólica e respiratória) em ambos os grupos. Os distúrbios acido-base nos neonatos do GD foram mais graves e sua recuperação mais lenta quando comparados com neonatos do GCE. Ao nascimento, neonatos no GD apresentaram maior hiperlactatemia e menor escore Apgar (pior vitalidade) em relação aos nascidos no GCE.

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Background: Emphysematous pyelonephritis (EPN) is an acute, severe necrotizing infection of the renal parenchyma and surrounding tissues that results in gas formation in the kidney, collecting system, or surroundings. EPN is a rare condition in veterinary medicine and occurs most frequently in dogs with diabetes mellitus. Although the prognosis of medical management in animals is poor, the standardized treatment protocol according to EPN severity is unclear. This report describes the first case of a nondiabetic female cat with extensive EPN and good prognosis following direct nephroureterectomy (NU). Case: A 10-year-old spayed female cat presented with the chief complaint of an acute loss of weight within 1 week, vomiting, and disorientation including stumbling, discoordination, circling, wobbling, head tilting, and difficulties in standing. At presentation, the patient had a body condition score of 1/9 and weighed 2.6 kg. Blood examination revealed leukocytosis, anemia, and hypoproteinemia. Abdominal radiography revealed severely decreased serosal details. A massive gas silhouette observed in the peritoneal and retroperitoneal cavities, was diagnosed as abdominal free gas. Abdominal ultrasound showed an accumulation of moderately anechoic fluid mixed with gas and cyst-like capsules around the left kidney. Left partial ureteral obstruction and dilation were also observed. Computed tomography (CT) was performed without sedatives or anesthetic drugs. The findings showed severe inflammatory changes in the peritoneum and a loss of the normal inner structure in the left kidney. A pyelogram of the left kidney was not observed after injection of the contrast material. Diffuse fat stranding and free gas observed in the mesentery of the entire abdominal cavity and around the left kidney were considered septic peritonitis. Urinalysis revealed proteinuria and hematuria. Numerous neutrophils with rod-type bacteria were observed in the ascites. Following diagnostic examinations, the patient was diagnosed with extensive left EPN, including inflammatory ascites and abdominal free gas. Therefore, emergency NU of the nonfunctional left kidney and ruptured ureter and thorough abdominal lavage were conducted. Diffuse inflammation and a nephrolith were observed in the section of the harvested kidney. The nephrolith was composed of 100% calcium oxalate monohydrate. The realtime polymerase chain reaction (RT-PCR) test for feline infectious peritonitis (FIP) was negative. Escherichia coli was detected in the ascites, and antibiotic therapy was administered following the antibiotic sensitivity test. The histological findings from the left kidney and ureter included marked chronic inflammation and fibrosis. The patient was discharged 4 days after surgery. During the 8-month follow-up period, the patient's condition improved. Discussion: This was a unique case of EPN in a nondiabetic cat and the first reported case of EPN with a ruptured ureter, including abdominal free gas, inflammatory ascites, and peritonitis. This patient had a bacterial urinary tract infection with E. coli, which is the most frequently isolated pathogen in humans. This gas-forming bacteria produced a massive amount of gas and inflammation that were considered to have ruptured the urinary tract, so that the gas was released into the abdomen. This case corresponded to class 3B, with two risk factors according to the human EPN classification system. Direct NU and abdominal lavage were performed as emergency surgeries. The patient stabilized gradually and showed a good prognosis. Immediate surgical intervention is recommended in animal patients showing the extensive EPN stage.

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Abstract This study determines the associations among serum lipid profiles, risk of cardiovascular disease, and persistent organic pollutants. Using Gas chromatography technique, the intensity of toxic pollutant residues in serum samples of Hypertensive patients were measured. Based on statistical analysis, the effects of different covariates namely pesticides, age, systolic blood pressure, diastolic blood pressure, and lipid profile duration was checked using the logistic regression model. Statistical computation was performed on SPSS 22.0. The P-values of F-Statistic for each lipid profile class are greater than 0.01 (1%), therefore we cannot reject the null hypothesis for all cases. The estimated coefficients, their standard errors, Wald Statistic, and odds ratio of the binary logistic regression model for different lipid profile parameters indicate if pesticides increase then the logit value of different lipid profile parameters changes from -0.46 to -0.246 except LDL which increases by 0.135. The study reports a significantly increased threat of cardiovascular disease with increased concentrations of toxic pollutants.

Resumo Este estudo determina as associações entre o perfil lipídico sérico, o risco de doença cardiovascular e os poluentes orgânicos persistentes. Por meio da técnica de cromatografia gasosa, mediu-se a intensidade dos resíduos de poluentes tóxicos em amostras de soro de pacientes hipertensos. Com base na análise estatística, os efeitos de diferentes covariáveis ​​- ou seja, pesticidas, idade, pressão arterial sistólica, pressão arterial diastólica e duração do perfil lipídico - foram verificados usando o modelo de regressão logística. O cálculo estatístico foi realizado no SPSS 22.0. Os valores P da estatística F para cada classe de perfil lipídico são maiores que 0,01 (1%), portanto não podemos rejeitar a hipótese nula para todos os casos. Os coeficientes estimados, seus erros padrão, estatística de Wald e odds ratio do modelo de regressão logística binária para diferentes parâmetros do perfil lipídico indicam se os pesticidas aumentam, então o valor logit de diferentes parâmetros do perfil lipídico muda de -0,46 para -0,246, exceto LDL, que aumenta em 0,135. O estudo relata um aumento significativo da ameaça de doença cardiovascular com aumento das concentrações de poluentes tóxicos.

Resumo

In some situations, the neonatal mortality rate in dogs can be high, and perinatal and postnatal veterinary care is essential to improve survival. This study to compared the Apgar score, serum lactate levels, and blood gas analysis results in 30 neonates born by elective cesarean section (GCE, n = 18) or cesarean section due to dystocia (GD, n = 12). Neonates were assessed at five time points: T0, at birth; T1, 3 h after birth; T2, 6 h after birth; T3, 12 h after birth; and T4, 24 h after birth. At T0, in the GCE group, 55.6% of the animals presented with adequate vitality, while 38.8% showed moderate changes in the Apgar score. In the GD group, 83.3% of the neonates had severe loss of vitality. At T1, none of the GCE neonates and 8.3% of the GD neonates presented with vitality deficits. Hyperlactatemia was observed in 83.3% of the GD neonates, and mixed acidosis (metabolic and respiratory) was observed at T0 in most neonates in both groups (GCE, 55.6%; GD, 72.7%). Compared to GCE neonates, the acid-base disorders were more severe and their recovery slower in GD neonates. At T0, GD neonates had higher hyperlactatemia and a lower Apgar score (worse vitality) than those of GCE neonates.

A taxa de mortalidade neonatal em cães pode ser elevada em algumas situações e a assistência veterinária peri e pós-natal mostra-se essencial para melhorar a sobrevivência dos filhotes nesse período. O objetivo deste estudo foi comparar o escore Apgar, lactato sérico e hemogasometria em neonatos nascidos por cesariana eletiva (GCE, n=18) e por cesariana após distocia (GD, n=12). Os neonatos foram avaliados em cinco momentos: (T0) ao nascimento; (T1) três horas; (T2) seis horas; (T3) 12 horas; e (T4) 24 horas. Ao nascimento, no GCE, 55,6% dos animais apresentaram boa vitalidade, de acordo com escore Apgar, e 38,8% demonstraram moderada vitalidade. No GD, 83,3% dos neonatos apresentaram baixa vitalidade. Ao T1 (três horas após o nascimento), nenhum (0%) dos neonatos do GCE e 8,3% dos neonatos do GD evidenciavam perda de vitalidade. Observou-se hiperlactatemia em 83,3% nos neonatos do GD e acidose mista (metabólica e respiratória) ao nascimento na maioria dos neonatos de ambos os grupos (55,6% no GCE e 72,7% no GD). Conclui-se que o principal distúrbio ácido-base observado ao nascimento foi acidose mista (metabólica e respiratória) em ambos os grupos. Os distúrbios acido-base nos neonatos do GD foram mais graves e sua recuperação mais lenta quando comparados com neonatos do GCE. Ao nascimento, neonatos no GD apresentaram maior hiperlactatemia e menor escore Apgar (pior vitalidade) em relação aos nascidos no GCE.

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Pregnancy toxemia has a high mortality rate and few cases with effective treatment are reported, especially when associated with secondary diseases. Therefore, the present work reports the case of a sheep, which was referred to the veterinary hospital of UFMG, in the last month of pregnancy, presenting apathy, hyporexia and sternal recumbency. After the diagnosis of pregnancy toxemia, the fetuses were removed while still alive, but did not survive. Monitoring was started with blood gas analysis, blood glucose assessments and laboratory tests. The results were consistent with liver damage, which was justified by the condition of lipolysis. Treatment consisted of electrolyte replacement plus 50% glucose and potassium chloride, along with administration of antibiotics (ceftiofur, 5mg/kg/SID/IM/7 days), flunixim meglumine (2.2mg/kg/SID/IM first day and 1.1mg/kg/SID/IM on the second and third days) and omeprazole (4mg/kg/SID/oral). The patient had secondarily clinical laminitis, which was treated with cryotherapy and anti-inflammatory drugs, in addition to mastitis, using a single dose of intramammary antibiotics. The work demonstrated the effectiveness of the treatment of pregnancy toxemia, when performed early and intensively, associated with daily clinical and laboratory monitoring of the animal.

A toxemia da prenhez possui alto índice de mortalidade, e poucos casos com tratamento eficaz são relatados, principalmente quando associados a doenças secundárias. Diante disso, o presente trabalho relata o caso de uma ovelha, que foi encaminhada ao hospital veterinário da UFMG, no último mês de gestação, apresentando apatia, hiporexia e decúbito esternal. Após o diagnóstico de toxemia da prenhez, os fetos foram retirados ainda vivos, porém não sobreviveram. Iniciou-se um monitoramento com análises hemogasométricas, avaliações de glicemia e exames laboratoriais. Os resultados foram condizentes com lesão hepática, a qual foi justificada pelo quadro de lipólise. O tratamento consistiu em reposição hidroeletrolítica associada à glicose 50% e a cloreto de potássio, juntamente com administração de antibióticos (ceftiofur, 5mg/kg/SID/IM/7 dias), flunixim meglumine (2,2mg/kg/SID/IM no primeiro dia e 1,1mg/kg/SID/IM no segundo e terceiro dias) e omeprazol (4mg/kg/SID/oral). A paciente apresentou secundariamente laminite clínica, a qual foi tratada com crioterapia e antinflamatórios, além de um quadro de mastite, sendo utilizado antibiótico intramamário em dose única. O trabalho demonstrou a eficácia do tratamento da toxemia da prenhez, quando realizado de forma precoce e intensiva, associado a um monitoramento clínico e laboratorial diário do animal.

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Background: Mammary tumors are the most common type of tumor in female dogs and account for 50% of all tumors in dogs. The clinical prognosis of canine mammary tumors is strongly affected by the size, stages, histological type, and grade of tumor; mitotic index; and nearby and distant metastasis. In canine mammary tumors, it is recommended that prognostic evaluation should also include complete blood count, serum biochemistry, and blood gases in addition to tumor size and stage. This study aimed to investigate the effect of tumor size, volume, and clinical stage on complete blood count, blood gas analysis, and serum biochemical parameters in bitches with mammary tumors and the correlation between them. Materials, Methods & Results: The study included a total of 18 bitches of different breeds, aged 6-15 years, of which 12 had mammary tumors and 6 were healthy. Thoracic X-rays were performed on bitches with mammary tumors in ventrodorsal and laterolateral positions to evaluate lung metastasis. Blood samples were collected from the cephalic vein from bitches in both groups in 2 different tubes (with plastic gel and ethylenediaminetetraacetic acid), 5 mL each, to perform complete blood count and evaluate blood gases and serum biochemical parameters. Blood samples were collected from the animals at the time of initial examination without any intervention. Analysis of the blood showed that bitches with mammary tumors had decreased levels of RBC, HCT, HGB, potassium, TCO2 , base excess, THbc, and ALT enzyme activity and increased levels of lactate, total protein, cholesterol, triglyceride, LDL, uric acid, and ALP and LDH enzyme activities compared with those in the control group. Furthermore, the dogs with a primary tumor of > 5 cm were found to have significantly higher levels of WBC, lactate, total protein, triglyceride, LDL, uric acid, and ALP and LDH enzyme activities and significantly lower levels of RBC and THbc compared with those in the control group. Bitches with tumors in multiple mammary lobes were found to have significantly higher levels of WBC, total protein, triglyceride, LDL, and ALP and LDH enzyme activities and significantly lower levels of RBC, HCT, HGB, TCO2 , THbc, and ALT enzyme activity compared with those in the control group. Based on the laboratory findings and approval of the owners of the dogs, mammary tissues containing the tumor and lymph nodes were surgically removed. After the operation, the removed mammary tissues were evaluated for size and volume. Clinical staging of the tumors was performed based on the size of the primary tumor (T), nearby lymph nodes (N), and metastasis (M) in accordance with the criteria set by WHO. Clinical staging of the tumors was, thus, based on the tumor, nodes, and metastases (TNM) score obtained according to the following system: Stage I: T1 N0 M0 , Stage II: T2 N0 M0 , Stage III: T3 N0 M0 , Stage IV: TanyN1 M0 , Stage V: made as TanyNanyM1 . Discussion: Mammary tumors are the most common type of neoplasm in bitches and, thus, cause serious problems in veterinary medicine. Tumors are significantly correlated with better prognosis compared with larger tumors. Based on this finding, this study investigated the effect of size, volume, and stage of mammary tumors in bitches on some blood parameters and the correlation between them. Therefore, it was concluded that clinical staging and evaluation of blood parameters could be useful in the diagnosis, treatment, and prediction of prognosis in canine mammary tumors. This study found that bitches with mammary tumors exhibited significant changes in their blood parameters (complete blood count, blood gas analysis, and serum biochemistry). The results obtained from this study may contribute to the development of approaches to the diagnosis, prediction of prognosis, and treatment of canine mammary tumors.

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Agoutis (Dasyprocta prymnolopha, WAGLER 1831) have been studied in captivity as a potential source of meat for rural populations in Brazil, as a way of providing low-cost protein and easing hunting pressures in local communities. However, little is known about their physiological blood markers, which are crucial for animal husbandry and effective welfare management. This study provides hemogasometric and ionic venous parameters for female agoutis raised in captivity in the drylands of Brazil. Venous blood samples were collected from six females and analyzed for dissolved gases and ions in the blood. Hemogasometric parameters, especially base excess and anion gap were generally heterogenous, while ions varied little among individuals. Moreover, we observed high chlorine values and negative base excess values, which suggest that some animals might be implementing compensatory mechanisms for ion imbalances. We provide a preliminary note on agouti venous gases and ions, while reiterating the importance of the clinical context and in-house parameter definition to maximize accuracy.(AU)

Cutias (Dasyprocta prymnolopha, WAGLER 1831) têm sido estudadas em cativeiro como uma fonte potencial de carne para populações rurais no Brasil, como uma forma de fornecer proteína de baixo custo e aliviar as pressões de caça nas comunidades locais. No entanto, pouco se sabe sobre seus marcadores sanguíneos fisiológicos os quais são cruciais para a criação animal e o manejo eficaz do bem-estar. Este estudo fornece parâmetros hemogasométricos e iônicos veno-sos para cutias fêmeas criadas em cativeiro em regiões áridas do Brasil. Amostras de sangue venoso foram coletadas de seis fêmeas e analisadas para gases dissolvidos e íons no sangue. Os parâmetros hemogasométricos, especialmente o excesso de base e o anion gap, foram heterogêneos, enquanto os íons variaram pouco entre os indivíduos. Além disso, altos valores de cloro e valores negativos de excesso de base foram observados o que sugere que alguns animais podem estar desenvol-vendo mecanismos compensatórios para desequilíbrios iônicos. Um perfil preliminar sobre gases e íons venosos de cutias foi fornecido, ao mesmo tempo, destaca-se a importância do contexto clínico e da definição interna dos parâmetros para maximizar a precisão.(AU)

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The effects on human beings of tear gas (CS) used by police forces during the control of civil disturbances are widely known and amply described in numerous scientific papers. However, the advent of the concepts of animal welfare raises the question of whether animals exposed to CS in such events, specifically horses, would suffer the same effects as those described for humans. The purpose of this study was to determine whether mounted police horses exposed to CS exhibit the same symptoms as humans. In this study, 12 horses of the Military Police of Paraná, healthy and with no history of respiratory tract disorders, were led through a gas cloud caused by the detonation of six teargas grenades. The horses' physiological parameters of respiratory rate, heart rate, rectal temperature, and color of the eye mucosa were evaluated 24 hours before exposure, 30 min, and 24 hours after exposure. Blood samples were collected for complete blood count (CBC) and blood gas analysis and samples of eye mucosa were obtained using sterile swabs. The analysis of these parameters did not reveal effects analogous to those described in humans.

Os efeitos sobre seres humanos do gás lacrimogêneo (CS) utilizado pelas forças policiais no controle de distúrbios civis são amplamente conhecidos e largamente descritos em numerosos artigos científicos. No entanto, com o advento dos conceitos de bem-estar animal, levanta-se a questão se animais expostos ao CS em tais eventos, especificamente cavalos, sofreriam os mesmos efeitos descritos em seres humanos. O objetivo deste estudo foi determinar se cavalos da polícia montada expostos ao CS apresentam os mesmos sintomas descritos em seres humanos. Neste estudo, 12 cavalos da Polícia Militar do Paraná, saudáveis e sem histórico de doença do trato respiratório, foram conduzidos por uma nuvem de gás causada pela detonação de seis granadas de gás lacrimogêneo. Os parâmetros fisiológicos de frequência respiratória, frequência cardíaca, temperatura retal e coloração das mucosas foram avaliados 24 horas antes da exposição, 30 min e 24 horas após a exposição. Amostras de sangue foram coletadas para realização de hemograma completo e hemogasometria, e amostras da mucosa ocular foram obtidas por meio de swabs estéreis. A análise desses parâmetros não revelou efeitos análogos aos descritos em seres humanos.

Resumo

As alterações do equilíbrio ácido-base são usualmente observadas por meio da hemogasometria, sendo esse exame fundamental para tratamento do paciente. O objetivo desta revisão é apresentar a importância da compreensão da teoria de eletroneutralidade e da sua representação gráfica (gamblegram), demonstrando sua aplicabilidade na rotina clínica. Para isso, dois casos clínicos em grandes animais são demonstrados. O primeiro caso é de um potro neonato com diarreia há seis dias, apresentando hiponatremia, hipocalemia e hipocloremia, com redução de SIDs, determinando acidose metabólica por fons fortes com redução de HCO3-. O gamblegram nos primeiros dias de internamento apresentou a influência da hiponatremia e hipercloremia relativa sobre a concentração de HCO3-. Inicialmente, fez-se a reposição de HCO3-, Ca," e K. O segundo caso é de um bovino adulto com refluxo abomasal, que apresentou hipocloremia, hipocalemia e normonatremia; as alterações em questão resultaram em alcalose metabólica iônica. O gamblegram foi eficaz na representação gráfica dessas alterações e da sua repercussão sobre o equilíbrio ácido-base de maneira sucinta. As alterações nas concentrações de eletrólitos merecem atenção e a reposição deve ser imediata e constante. O gamblegram é uma ferramenta útil para os clínicos, sendo capaz de representar alterações complexas de maneira prática.

Changes in the acid-base balance are usually observed using blood gas analysis, and this test is essential for the treatment of the patient. The aim of this review is to present the importance of understanding the theory of electroneutrality and its graphical representation (gamblegram), demonstrating its applicability in clinical routine. For this, two clinical cases in large animals are discussed. The first case, a neonate foal with diarrhea during six days, presenting hyponatremia, hypokalemia and hypochloremia, with a reduction in SID, determining metabolic acidosis by strong ions with a reduction in HCO3-. The gamblegram in the first days of hospitalization showed the influence of hyponatremia and relative hyperchloremia on the concentration of HCO3-. Initially, HCO3-, Ca²** and K' were corrected. The second case, adult cattle with abomasal reflux presented hypochloremia, hypokalemia and normonatremia, the alterations in question resulting in ionic metabolic alkalosis. The gamblegram was effective in representing these changes and their impact on the acid-base balance. Changes in electrolyte concentrations deserve attention and replacement must be immediate and constant. The gamblegram is a useful tool for clinicians, being able to represent complex changes in a practical way.

Resumo

Ozone therapy is a technique used in several specialties of equine medicine; however, there are few studies on its use in vaquejada (cowboy competition) athlete horses. This study aims to evaluate the potential effect of ozone gas administered by two different routes on hematological and biochemical values and the oxidative stress marker in vaquejada athlete horses. For this, nine healthy equines that followed a training protocol and underwent two treatments were used with an 8-day wash-out between them. The major ozonated autohemotherapy (MOA) treatment group received a volume of 600ml of the 02-03 mixture at a concentration of 60 µg/mL, and the rectal insufflation (RI) treatment group received 5mL of gas per kg of body weight at a concentration of 15µg/kg performed every 24h on three consecutive days. Results were significant for RBC, hematocrit, and hemoglobin in the hematological variables, and AST and lactate for biochemical and malondialdehyde variables. No statistically significant differences were found in comparisons between treatment groups. Thus, we can conclude that there is no difference between the two therapies, indicating that the two techniques are effective for the application of ozone therapy in horses competing for vaquejada.

A ozonioterapia é uma técnica utilizada em diversas especialidades da medicina equina, contudo, são escassos os estudos de sua utilização em cavalos atletas de vaquejada. O presente estudo tem por objetivo avaliar o potencial efeito do gás ozônio administrado por duas diferentes vias sobre os valores hematológicos, bioquímicos e no marcador de estresse oxidativo em cavalos atletas de vaquejada. Para isso, foram utilizados 9 equinos hígidos que seguiram um protocolo de treinamento e foram submetidos a dois tratamentos, com um wash-out de 8 dias entre eles. O grupo de tratamento auto-hemoterapia maior ozonizada (AHTMO) recebeu um volume de 600ml da mistura 02-03 na concentração de 60 µg/mL e o grupo de tratamento insuflação retal (IR) recebeu 5mL de gás por kg de peso vivo, na concentração de 15µg/kg, realizado a cada 24h em três dias consecutivos. Os resultados demonstraram-se significativos para hemácias, hematocrito e hemoglobina nas variáveis hematológicas, AST e lactato para as variáveis bioquímicas e para o malondialdeido. Não foram encontradas diferenças estatísticas significativas nas comparações entre grupos de tratamento. Assim, pode-se concluir que não há diferença entre as duas terapias, indicando que as duas técnicas são eficazes para a aplicação da ozonioterapia em cavalos competidores de vaquejada.