Bradyrhizobium and Azospirillum co-inoculation associated with cobalt and molybdenum application in the soybean crop / Co-inoculação de Bradyrhizobium e Azospirillum associadas a aplicação de cobalto e molibdênio na cultura da soja

ABSTRACT: Co-inoculation between bacteria of the genera Bradyrhizobium and Azospirillum can enhance the nodulation and promote the development of the soybean [Glycine max (L.) Merrill] root system, contributing to the increase in grain yield, in addition to the reduction in production costs and contamination of natural resources. Cobalt (Co) and molybdenum (Mo) use can also favor biological nitrogen fixation. The research evaluated the co-inoculation effect of bacteria associated with the Co and Mo application in soybean crop. The randomized blocks design was employed, in a 2 x 6 factorial scheme, presence and absence of Co and Mo and five ways of using the products Bradyrhizobium and Azospirillum, plus control, with four replications. The treatments were formed by the control (not inoculated + 20 kg N ha-1); seed inoculation with Bradyrhizobium (100 mL ha-1) + 20 kg N ha-1; seed inoculation with Bradyrhizobium (100 mL ha-1) and three treatments applying Bradyrhizobium + Azospirillum in furrow, in different doses. Height of insertion of the first pod, total number of pods and grains per plant, weight of 100 grains and grain yield were evaluated. Inoculation of Bradyrhizobiumjaponicum associated with co-inoculation of Azospirillumbrasilense via foliar and Co and Mo, provided increases in the number of pods per plant, number of grains per pod and weight of 100 grains, reflecting increases in grain yield.The use of Co and Mo, on average, increased soybean yield by 10%, resulting in an average yield of 4,904 kg ha-1.

RESUMO: A co-inoculação entre bactérias do gênero Bradyrhizobium e Azospirillum pode potencializar a nodulação e promover o desenvolvimento do sistema radicular da soja [Glycine max (L.) Merrill], contribuindo com o aumento na produtividade de grãos, além da redução nos custos de produção e contaminação dos recursos naturais. A utilização de cobalto (Co) e molibdênio (Mo), também pode favorecer a fixação biológica de nitrogênio. O objetivo do trabalho foi avaliar o efeito da co-inoculação de bactérias associadas a aplicação de Co e Mo na cultura da soja. O delineamento utilizado foi de blocos casualizados, em esquema fatorial 2 x 6, na presença e ausência de Co e Mo e cinco formas de utilização dos produtos Bradyrhizobium e Azospirillum, mais controle, com quatro repetições. Os tratamentos foram formados pela testemunha (não inoculada + 20 kg de N ha-1); inoculação com Bradyrhizobium na semente (100 mL ha-1) + 20 kg de N ha-1; inoculação de sementes com Bradyrhizobium (100 mL ha-1) e três tratamentos aplicando Bradyrhizobium + Azospirillum em sulco em diferentes doses. Foram avaliados altura de inserção da primeira vagem, número total de vagens e grãos por planta, massa de 100 grãos e produtividade de grãos. A inoculação de Bradyrhizobium japonicum associado a co-inoculação de Azospirillum brasilense via foliar e ao Co e Mo, proporcionou incrementos no número de vagens por planta, número de grãos por vagem e massa de 100 grãos, refletindo acréscimos na produtividade de grãos. O uso de Co e Mo, em média, aumentou o rendimento da soja em 10%, resultando numa produtividade média de 4904 kg ha-1.

Evaluation of antibacterial and antioxidant activity of purple araçá essential oil (Psidium rufum, Myrtaceae) / Avaliação das atividades antibacteriana e antioxidante do óleo essencial do araçá-roxo (Psidium rufum, Myrtaceae)

The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil (EO) from fresh leaves of Psidium rufum. The EO was extracted by hydrodistillation and identified by gas chromatography coupled to mass spectrometry. The antibacterial activity was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Antioxidant activity was determined by ß-carotene/linoleic acid co-oxidation system, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and iron reduction methods. Hydrocarbon sesquiterpenes were the predominant class, indicating 1,8 cineole, α-longipinene as major. The EO was tested against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa (MIC = 2,500 µg/mL and MBC = 20,000 µg/mL); Enterococcus faecalis (MIC = 2,500µg/mL and MBC > 20,000µg/mL) and Escherichia coli (MIC > 20,000µg/mL and MBC > 20,000µg/mL). The EO showed antioxidant potential due to ß-carotene/linoleic acid co-oxidation system, with 76.63% of oxidation inhibition (1.0mg/mL) and due to the iron reduction power (5,38 µmol Fe 2+ /mg sample). The results are promising in recommending this species for the development of food, cosmetic and pharmaceutical products.

O objetivo deste estudo foi avaliar as atividades antibacteriana e antioxidante do óleo essencial (OE) das folhas frescas de Psidium rufum. O OE foi extraído por hidrodestilação e identificado por cromatografia gasosa acoplada à espectrometria de massas. Foi avaliada a atividade antibacteriana, determinando-se a concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM). A atividade antioxidante foi determinada pelo sistema de co-oxidação ß-caroteno/ácido linoleico, pelos métodos de sequestro do radical 2,2-difenil-1-picrilhidrazil e de redução do ferro. Sesquiterpenos hidrocarbonetos foram a classe predominante, indicando 1,8 cineol, α-longipineno como majoritário. O OE foi testado contra as bactérias Staphylococcus aureus e Pseudomonas aeruginosa (CIM = 2,500µg/mL e CBM = 20.000µg/mL); Enterococcus faecalis (CIM=2,500µg/mL e CBM > 20.000µg/mL) e Escherichia coli (CIM > 20.000µg/mL e CBM > 20.000µg/mL). O OE apresentou potencial antioxidante pelo sistema co-oxidação ß-caroteno/ácido linoleico com 76,63% de inibição da oxidação (1,0mg/mL) e pelo poder de redução de ferro (5,38 µmol Fe2+/mg amostra). Os resultados são promissores em indicar essa espécie para o desenvolvimento de produtos alimentícios, cosméticos e farmacêuticos.

Production and marketing of Tuber floridanum ­ ecology and gastronomic value of a recently described truffle species

Tuber floridanum is the first truffle species reported in Brazil and little is known about its nutritional properties and ecology. Therefore, this study aimed to (1) determine T. floridanum aroma and nutritional properties and (2) analyze cultivation and fruiting conditions, including potential crop pests and market value. Fruiting bodies of T. floridanum showed a slightly ellipsoid shape and smooth to shallow groovy surface. The mass of ascocarps ranged from 0.13 g to 15.95 g, and the truffle was composed of 71 % carbohydrates, 19 % protein, 5 % ash, and 5 % of fat. Octan-3-one, 1-methoxy-3-methyl-butane, and 1-methoxy-2-methyl-butane determined T. floridanum characteristic aroma. Soil properties were also specific for this truffle, differing from optimal soils where commercial truffles are produced. In addition, the occurrence of earwigs (Anisolabididae, Anisolabidinae) feeding on truffles (26 % of all ascocarps) in pecan orchards may reduce truffle quality and cause financial losses. T. floridanum aroma has a unique composition, completely different from any commercial and non-commercial truffle species analyzed so far. Soil conditions in pecan orchards were also specific for truffle fruiting. Further studies should elucidate other ecological conditions for the co-production of T. floridanum with pecan.(AU)

Anaplasma phagocytophilum in horses - evaluation of proinflammatory biomarkers

Background: Anaplasma phagocytophilum is an obligate intracellular pathogen transmitted by the ticks that cause equinegranulocytic anaplasmosis (EGA). This pathogen is infects predominantly blood cells, principally granulocytes and especially neutrophils. A. phagocytophilum causes an acute febrile disease in horses accompanying with lethargy, loss ofappetite, lameness and hemorrhages. In horses, this disease should be considered in all acute symptoms accompaniedby thrombocytopenia and leukopenia identified by hematological test performed. Tick-borne pathogens have becomeincreasingly threatening for both animals and also public health since ticks mostly carry numerous well-documented andundocumented pathogens, and the geographical range of ticks has expanded in the recent years. This research has aimed toevaluate the impact of A. phagocytophilum infection on some oxidative/nitrosative stress parameters, antioxidant enzymeactivities, proinflammatory biomarkers and trace element levels in horses.Materials, Methods & Results: The present study has been carried out using blood samples collected from 93 horses aged1-year and older. The blood samples were centrifuged and sera were separated. Serum samples stored in the freezer (-20°C)until the day of analysis. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNAgene of A. phagocytophilum and then positive PCR products were sequenced. A. phagocytophilum was 6 horses (6.4%)showed positive nested-PCR results. An infected group comprised of 6 positive horses according to PCR analysis results also6 healthy horses as control were selected. Serum SOD (Horse Superoxide Dismutase(Cu-Zn)) ELISA Kit, MPO (ELISAAssay Kit Horse Myeloperoxidase) and GPx (Horse glutathione peroxidase 1 ELISA Kit Assay), IL1 (Horse Interleukin 1Beta ELISA Kit), IL6 (Horse Interleukin 6 ELISA Kit), TNF α (Horse Tumor Necrosis...

Characterization and effects of DDG on the intake and digestibility of finishing bulls in feedlots

The aim of this study was to characterize four corn and sorghum co-products (DDG) in terms of their protein and carbohydrate fractions; we also evaluated the effects of substituting the protein source of the conventional supplement by DDG on consumption and nutrient digestibility in confined finishing cattle. Thirty-six male Nellore cattle with a mean age of 24 months were used. The treatments were: FA: concentrate with corn as an energy source and cottonseed meal as a protein source; DDG50: concentrate with a 50% substitution of the FA protein source by DDG; DDG100: concentrate with 100% substitution of the FA protein source by DDG. The experimental design was completely randomized with three treatments and three replicates (pens) containing four animals per pen. We found that the use of DDG in the finishing phase did not interfere with the animals food intake or the digestibility of the nutrients (p > 0.05). Nutrients were used by the animals; therefore, DDGs may be viable substitutes of cottonseed meal. We conclude that the bromatological composition of this co-product is influenced a lot during processing; therefore, the nutritional values of this co-product present in the composition tables may not be true.(AU)

Characterization and effects of DDG on the intake and digestibility of finishing bulls in feedlots

The aim of this study was to characterize four corn and sorghum co-products (DDG) in terms of their protein and carbohydrate fractions; we also evaluated the effects of substituting the protein source of the conventional supplement by DDG on consumption and nutrient digestibility in confined finishing cattle. Thirty-six male Nellore cattle with a mean age of 24 months were used. The treatments were: FA: concentrate with corn as an energy source and cottonseed meal as a protein source; DDG50: concentrate with a 50% substitution of the FA protein source by DDG; DDG100: concentrate with 100% substitution of the FA protein source by DDG. The experimental design was completely randomized with three treatments and three replicates (pens) containing four animals per pen. We found that the use of DDG in the finishing phase did not interfere with the animals’ food intake or the digestibility of the nutrients (p > 0.05). Nutrients were used by the animals; therefore, DDGs may be viable substitutes of cottonseed meal. We conclude that the bromatological composition of this co-product is influenced a lot during processing; therefore, the nutritional values of this co-product present in the composition tables may not be true.

Subproduct of the Wine Industry to Replace Forage for Feeding Confined Ovine

ABSTRACT The aim of the present study was to evaluate the ingestive behavior of sheep receiving alternative food with grape pomace to replace roughage, as well as its impacts on the rumen environment. It was observed that BU had higher TOC, TRAM and pH of the ruminal liquid, while there was less TRU (P 0,05). As for the other parameters, no significant differences were found. In this study, just as the feeding time was not influenced due to the similarity of the concentrate: roughage ratio in the diet, the protozoan count did not change. This finding reinforces the possibility of using grape marc as a tool for formulating feed, reducing the risk of ruminal disturbances. It was observed that the group control had lower TRAM, indicating that the diet of this group promoted greater microbial activity and, consequently, greater digestibility, corroborating the already observed TOC. This result was the opposite of what was expected, since the grape pomace has probiotic properties, precisely because the fruits have more fungi and bacteria in their microbiota. This also confirms that the co-products of vitiviniculture can act as promoters of the intestinal health of ruminants, justifying further studies in order to improve this use. Therefore, the use of wine by-products is an alternative to reduce production costs, as its use in diets for confined sheep can improve the performance of the animals, increasing the total feed consumption and improving microbial activity.

RESUMO O objetivo do presente estudo foi avaliar o comportamento ingestivo de ovinos recebendo ração alternativa com bagaço de uva em substituição ao volumoso, bem como seus impactos no ambiente ruminal. Observou-se que a BU apresentou maiores COT, TRAM e pH do líquido ruminal, enquanto houve menor TRU (P 0,05). Quanto aos demais parâmetros, não foram encontradas diferenças significativas. Neste estudo, assim como o tempo de alimentação não foi influenciado pela similaridade da relação concentrado: volumoso na dieta, a contagem de protozoários não se alterou. Esse achado reforça a possibilidade do uso do bagaço de uva como ferramenta na formulação de rações, reduzindo o risco de distúrbios ruminais. Observou-se que o grupo controle apresentou menor TRAM, indicando que a dieta desse grupo promoveu maior atividade microbiana e, consequentemente, maior digestibilidade, corroborando o COT já observado. Esse resultado foi o oposto do esperado, já que o bagaço de uva tem propriedades probióticas, justamente porque os frutos possuem mais fungos e bactérias em sua microbiota. Isso também confirma que os coprodutos da vitivinicultura podem atuar como promotores da saúde intestinal de ruminantes, justificando novos estudos no sentido de aprimorar esse uso. Portanto, a utilização de subprodutos do vinho é uma alternativa para reduzir os custos de produção, pois seu uso em dietas para ovinos confinados pode melhorar o desempenho dos animais, aumentando o consumo total de ração e melhorando a atividade microbiana.

Effect of the diet on performance characteristics and quality of meat fat of lamb by principal component analysis

The objective of this study was to evaluate the effect of use of agroindustrial co-products in the diet of lambs on the performance and nutritional quality of meat fat. Twenty lambs were distributed into four experimental groups: control diet (C) or diets containing cottonseed (CS), sunflower meal (SFM), and castor cake (CC). During the experimental period, data on dry matter consumption (DMC) and total weight gain (TWG) were collected. After slaughter, samples of Logissimus dorsi (LD) muscle were taken for fatty acids and cholesterol analysis. Diets C and CS provided similar characteristics of fat quality and animal performance. However, SFM and CC diets tend to form isolated groups, with different fat and performance characteristics. The SFM diet results in meat with a higher saturated fatty acids and cholesterol content. The CC diet is related to improved performance characteristics and lower cholesterol content. In conclusion, the inclusion of CC in the experimental diets provided, in the proportions used in this experiment, better characteristics of animal performance and meat fat quality.(AU)

Effect of the diet on performance characteristics and quality of meat fat of lamb by principal component analysis

The objective of this study was to evaluate the effect of use of agroindustrial co-products in the diet of lambs on the performance and nutritional quality of meat fat. Twenty lambs were distributed into four experimental groups: control diet (C) or diets containing cottonseed (CS), sunflower meal (SFM), and castor cake (CC). During the experimental period, data on dry matter consumption (DMC) and total weight gain (TWG) were collected. After slaughter, samples of Logissimus dorsi (LD) muscle were taken for fatty acids and cholesterol analysis. Diets C and CS provided similar characteristics of fat quality and animal performance. However, SFM and CC diets tend to form isolated groups, with different fat and performance characteristics. The SFM diet results in meat with a higher saturated fatty acids and cholesterol content. The CC diet is related to improved performance characteristics and lower cholesterol content. In conclusion, the inclusion of CC in the experimental diets provided, in the proportions used in this experiment, better characteristics of animal performance and meat fat quality.

Subproduct of the Wine Industry to Replace Forage for Feeding Confined Ovine / Coproduto da Indústria de Vitivinificação como Substituto ao Volumoso na Alimentação de Ovinos Confinados

The aim of the present study was to evaluate the ingestive behavior of sheep receiving alternative food with grape pomace to replace roughage, as well as its impacts on the rumen environment. It was observed that BU had higher TOC, TRAM and pH of the ruminal liquid, while there was less TRU (P < 0,05). As for the other parameters, no significant differences were found. In this study, just as the feeding time was not influenced due to the similarity of the concentrate: roughage ratio in the diet, the protozoan count did not change. This finding reinforces the possibility of using grape marc as a tool for formulating feed, reducing the risk of ruminal disturbances. It was observed that the group control had lower TRAM, indicating that the diet of this group promoted greater microbial activity and, consequently, greater digestibility, corroborating the already observed TOC. This result was the opposite of what was expected, since the grape pomace has probiotic properties, precisely because the fruits have more fungi and bacteria in their microbiota. This also confirms that the co-products of vitiviniculture can act as promoters of the intestinal health of ruminants, justifying further studies in order to improve this use. Therefore, the use of wine by-products is an alternative to reduce production costs, as its use in diets for confined sheep can improve the performance of the animals, increasing the total feed consumption and improving microbial activity.(AU)

O objetivo do presente estudo foi avaliar o comportamento ingestivo de ovinos recebendo ração alternativa com bagaço de uva em substituição ao volumoso, bem como seus impactos no ambiente ruminal. Observou-se que a BU apresentou maiores COT, TRAM e pH do líquido ruminal, enquanto houve menor TRU (P <0,05). Quanto aos demais parâmetros, não foram encontradas diferenças significativas. Neste estudo, assim como o tempo de alimentação não foi influenciado pela similaridade da relação concentrado: volumoso na dieta, a contagem de protozoários não se alterou. Esse achado reforça a possibilidade do uso do bagaço de uva como ferramenta na formulação de rações, reduzindo o risco de distúrbios ruminais. Observou-se que o grupo controle apresentou menor TRAM, indicando que a dieta desse grupo promoveu maior atividade microbiana e, consequentemente, maior digestibilidade, corroborando o COT já observado. Esse resultado foi o oposto do esperado, já que o bagaço de uva tem propriedades probióticas, justamente porque os frutos possuem mais fungos e bactérias em sua microbiota. Isso também confirma que os coprodutos da vitivinicultura podem atuar como promotores da saúde intestinal de ruminantes, justificando novos estudos no sentido de aprimorar esse uso. Portanto, a utilização de subprodutos do vinho é uma alternativa para reduzir os custos de produção, pois seu uso em dietas para ovinos confinados pode melhorar o desempenho dos animais, aumentando o consumo total de ração e melhorando a atividade microbiana.(AU)

Influência de diferentes fontes e níveis de fibra nadieta de juvenis de pacu (Piaractus mesopotamicus) sobre o desempenho zootécnico, parâmetros somáticos e composição centesimal / Influence of different sources and fiber levels in the diet of juvenile pacu (Piaractus mesopotamicus) on the growth performance, somatic parameters and proximate composition

O objetivo do presente trabalho foi avaliar os efeitos da casca de soja e da casca de arroz sobre o desempenho zootécnico, parâmetros somáticos e composiçãocentesimal dos peixes inteiros de juvenis de pacu (Piaractus mesopotamicus). Para isso, foram utilizados 216 juvenis de pacu, com peso médio de 6 ± 0,81 gramas, distribuídos em 18 caixas d’água, em sistema de recirculação. Foram formuladas seis dietas contendo os níveis de 10, 13 e 15% de inclusão de FDN para cada fonte de fibra, com 30% de proteína bruta e 3.000 Kcal Kg-1de energia digestível. Foi observado que os níveis de 10 e 13% de FDN promoveram os melhores resultados para as variáveis de desempenhocomo peso final, ganho em peso, taxa de crescimento específico e comprimento final. Também foi possível observar que os peixes alimentados com a dieta contendo 10% de FDN apresentaram um maior rendimento de carcaça, porém não foi observada diferença entreos índices,digestivo somático, hepatossomático e quociente intestinal. Já, para as variáveis de composição centesimal dos peixes inteiros, não foram observadas diferenças significativas. Sendo assim, pode-se concluir que até 13% de FDN não prejudica o desempenho zootécnico, qualidade de carcaça e os parâmetros somáticos dos peixes.

The objective of the present work was to evaluate the effects of soybean husk and rice husk on the performance, somatic parameters and proximate composition of whole fish of juvenile pacu (Piaractus mesopotamicus). For this, 216 juveniles of pacu were used, with an average weight of 6 ± 0.81 grams, distributed in 18 water tanks, in a recirculation system. Six diets were formulated containing levels of 10, 13 and 15% of NDF inclusion for each fiber source, with 30% of crude protein and 3,000 Kcal Kg-1of digestible energy. It was observed that the levels of 10 and 13% of NDF promoted the best results for performance variables such as final weight, weight gain, specific growth rate and final length. It was also possible to observe that fish fed a diet containing 10% NDF had a higher carcass yield, but no difference was observed between the somatic, hepatosomatic and intestinal quotient digestive indexes. However, no significant differences were observed for the proximate composition variables of whole fish. Thus, it can be concluded that up to 13% of NDF does not affect the zootechnical performance, carcass quality,and somatic parameters of the fish.

Influência de diferentes fontes e níveis de fibra nadieta de juvenis de pacu (Piaractus mesopotamicus) sobre o desempenho zootécnico, parâmetros somáticos e composição centesimal / Influence of different sources and fiber levels in the diet of juvenile pacu (Piaractus mesopotamicus) on the growth performance, somatic parameters and proximate composition

O objetivo do presente trabalho foi avaliar os efeitos da casca de soja e da casca de arroz sobre o desempenho zootécnico, parâmetros somáticos e composiçãocentesimal dos peixes inteiros de juvenis de pacu (Piaractus mesopotamicus). Para isso, foram utilizados 216 juvenis de pacu, com peso médio de 6 ± 0,81 gramas, distribuídos em 18 caixas dágua, em sistema de recirculação. Foram formuladas seis dietas contendo os níveis de 10, 13 e 15% de inclusão de FDN para cada fonte de fibra, com 30% de proteína bruta e 3.000 Kcal Kg-1de energia digestível. Foi observado que os níveis de 10 e 13% de FDN promoveram os melhores resultados para as variáveis de desempenhocomo peso final, ganho em peso, taxa de crescimento específico e comprimento final. Também foi possível observar que os peixes alimentados com a dieta contendo 10% de FDN apresentaram um maior rendimento de carcaça, porém não foi observada diferença entreos índices,digestivo somático, hepatossomático e quociente intestinal. Já, para as variáveis de composição centesimal dos peixes inteiros, não foram observadas diferenças significativas. Sendo assim, pode-se concluir que até 13% de FDN não prejudica o desempenho zootécnico, qualidade de carcaça e os parâmetros somáticos dos peixes.(AU)

The objective of the present work was to evaluate the effects of soybean husk and rice husk on the performance, somatic parameters and proximate composition of whole fish of juvenile pacu (Piaractus mesopotamicus). For this, 216 juveniles of pacu were used, with an average weight of 6 ± 0.81 grams, distributed in 18 water tanks, in a recirculation system. Six diets were formulated containing levels of 10, 13 and 15% of NDF inclusion for each fiber source, with 30% of crude protein and 3,000 Kcal Kg-1of digestible energy. It was observed that the levels of 10 and 13% of NDF promoted the best results for performance variables such as final weight, weight gain, specific growth rate and final length. It was also possible to observe that fish fed a diet containing 10% NDF had a higher carcass yield, but no difference was observed between the somatic, hepatosomatic and intestinal quotient digestive indexes. However, no significant differences were observed for the proximate composition variables of whole fish. Thus, it can be concluded that up to 13% of NDF does not affect the zootechnical performance, carcass quality,and somatic parameters of the fish.(AU)

Microencapsulation and co-encapsulation of bioactive compounds for application in food: challenges and perspectives / Microencapsulação e co-encapsulação de compostos bioativos para aplicação em alimentos: desafios e perspectivas

The availability of different food products containing bioactive compounds promotes their inclusion in the daily diet of consumers. However, the effective and safe delivery of such products requires certain precautions to ensure their preservation, stability, and bioavailability when consumed. Microencapsulation is a great alternative, which is a method capable of protecting different bioactive compounds, including probiotic cells, prebiotic compounds, and some antioxidant substances such as phenolic compounds, anthocyanins, flavonoids, and vitamins. Therefore, this study aimed to perform a literature review and present different alternatives to make bioactive compounds viable through microencapsulation, increase their stability and viability when applied in different food matrices, and address the existing challenges regarding their effectiveness.(AU)

A oferta de diferentes produtos alimentícios que contenham compostos bioativos facilita a sua inserção na dieta como parte do dia a dia do consumidor. No entanto, para que estes compostos sejam entregues de forma segura e eficaz, o uso de certos meios se torna necessário para garantir sua preservação, estabilidade e biodisponibilidade quando consumidos. Com esta finalidade, apresenta-se como uma grande alterativa a microencapsulação, que é um método capaz de fornecer proteção a diferentes compostos bioativos, que incluem células probióticas, compostos prebióticos, e algumas substâncias antioxidantes como compostos fenólicos, antocianinas, flavonoides, vitaminas, dentre outros e garantir uma melhor efetividade na sua entrega. O objetivo deste trabalho foi realizar uma revisão apresentando formas de viabilizar os compostos bioativos através da microencapsulação, para aumentar sua estabilidade e viabilidade diante da aplicação em diferentes matrizes alimentícias, além de abordar os desafios existentes para a sua efetividade.(AU)

Outbreak of organophosphorus compound-induced delayed neurotoxicity in water buffaloes / Surto de neuropatia tardia induzida por organofosforados em búfalos

Forty 1-2-y-old water buffaloes were simultaneously treated with trichlorfon and chlorpyrifos products in the recommended dose for cattle. After a week, 19 animals started presenting clinical signs characterized by apathy, diarrhea, aggressiveness, dehydration, and motor incoordination, followed by flaccid paralysis and permanent lateral recumbency. All affected buffaloes died after a clinical course of 1-4 days. Reduction of serum cholinesterase activity in three cases was indicative of significant exposure to organophosphorus compounds (OPs). Pathological examination of three buffaloes revealed no gross and histological lesions. By thin layer chromatography, chlorpyrifos residues and trace of trichlorfon residues were detected in fresh tissue samples. The epidemiological, clinical, pathological, and toxicological findings were highly compatible with OPs-induced delayed neurotoxicity, a neurological manifestation rarely described in domestic animals.

Quarenta búfalos foram simultaneamente tratados com clorpirifós e triclorfom na dose recomendada para bovinos. Após uma semana, 19 animais apresentaram sinais clínicos caracterizados por apatia, diarreia, agressividade, desidratação e incoordenação motora, seguidos por paralisia flácida e decúbito lateral permanente. Todos os búfalos afetados morreram após um curso clínico de 1-4 dias. Redução da atividade da colinesterase sérica em três casos foi indicativa de exposição significativa a organofosforados (OPs). O exame patológico de três búfalos não revelou lesões macroscópicas e histológicas. Por cromatografia em camada delgada, resíduos de clorpirifós e traços de resíduos de triclorfon foram detectados em amostras de tecidos frescos. Os achados epidemiológicos, clínicos, patológicos e toxicológicos foram compatíveis com neuropatia tardia induzida por OPs, uma manifestação neurológica raramente descrita em animais domésticos.

Outbreak of organophosphorus compound-induced delayed neurotoxicity in water buffaloes / Surto de neuropatia tardia induzida por organofosforados em búfalos

Forty 1-2-y-old water buffaloes were simultaneously treated with trichlorfon and chlorpyrifos products in the recommended dose for cattle. After a week, 19 animals started presenting clinical signs characterized by apathy, diarrhea, aggressiveness, dehydration, and motor incoordination, followed by flaccid paralysis and permanent lateral recumbency. All affected buffaloes died after a clinical course of 1-4 days. Reduction of serum cholinesterase activity in three cases was indicative of significant exposure to organophosphorus compounds (OPs). Pathological examination of three buffaloes revealed no gross and histological lesions. By thin layer chromatography, chlorpyrifos residues and trace of trichlorfon residues were detected in fresh tissue samples. The epidemiological, clinical, pathological, and toxicological findings were highly compatible with OPs-induced delayed neurotoxicity, a neurological manifestation rarely described in domestic animals.(AU)

Quarenta búfalos foram simultaneamente tratados com clorpirifós e triclorfom na dose recomendada para bovinos. Após uma semana, 19 animais apresentaram sinais clínicos caracterizados por apatia, diarreia, agressividade, desidratação e incoordenação motora, seguidos por paralisia flácida e decúbito lateral permanente. Todos os búfalos afetados morreram após um curso clínico de 1-4 dias. Redução da atividade da colinesterase sérica em três casos foi indicativa de exposição significativa a organofosforados (OPs). O exame patológico de três búfalos não revelou lesões macroscópicas e histológicas. Por cromatografia em camada delgada, resíduos de clorpirifós e traços de resíduos de triclorfon foram detectados em amostras de tecidos frescos. Os achados epidemiológicos, clínicos, patológicos e toxicológicos foram compatíveis com neuropatia tardia induzida por OPs, uma manifestação neurológica raramente descrita em animais domésticos.(AU)

Biodiesel co-products modified the rumen parameters of feedlot lambs but did not change methane production in vitro

Background: Intensification of livestock is a strategy that increases productivity, but the diets used to increase animal production efficiency are composed mainly of corn and soybean, thereby increasing competition between animals and humansfor the same food crops. This study evaluated nutrient intake, apparent digestibility of dry matter (DM) and nutrients, kinetics of gas production, and concentration of volatile fatty acids on diets with or without inclusion of biodiesel co-productsformulated for feedlot lambs. So, the hypothesis is that replace of traditional ingredients by biodiesel co-products changesrumen parameters and methane emissions.Materials, Methods & Results: The experiment was developed in São Paulo State University (Unesp), Jaboticabal, SP,Brazil, in Sheep Production Laboratory, which is owned to Animal Science Department. All trials developed in this studyused a feedlot system, where animals were kept in individual pen. Forty Ile de France lambs male non-castrated wereused in in vivo trial. To obtain rumen fluid, that was used in in vitro trial, four Santa Inês lambs with rumen cannula wereused. The treatments evaluated were four diets: Control diet: roughage + concentrate; PM20: roughage + concentrate withpeanut meal (PM) at 20% of DM; CG25: roughage + concentrate with crude glycerin (CG) at 25% of DM; and PMCG:roughage + concentrate with PM at 10% of DM and CG at 12.5% of DM. The roughage:concentrate ratio was 40:60 for allthese diets. The parameters of the in vitro and in vivo experiments used were completely randomized with four treatments.When significant, the means between treatments were compared using Tukey test (P < 0.05). There was no effect of coproduct inclusion on intake, except ether extract and neutral detergent fiber, which were higher for PM20 compared withCG25 diet. Apparent digestibility of dry matter (79.87%) and some nutrients (organic matter, crude protein, and neutraldetergent fiber)...(AU)

Biodiesel co-products modified the rumen parameters of feedlot lambs but did not change methane production in vitro

Background: Intensification of livestock is a strategy that increases productivity, but the diets used to increase animal production efficiency are composed mainly of corn and soybean, thereby increasing competition between animals and humansfor the same food crops. This study evaluated nutrient intake, apparent digestibility of dry matter (DM) and nutrients, kinetics of gas production, and concentration of volatile fatty acids on diets with or without inclusion of biodiesel co-productsformulated for feedlot lambs. So, the hypothesis is that replace of traditional ingredients by biodiesel co-products changesrumen parameters and methane emissions.Materials, Methods & Results: The experiment was developed in São Paulo State University (Unesp), Jaboticabal, SP,Brazil, in Sheep Production Laboratory, which is owned to Animal Science Department. All trials developed in this studyused a feedlot system, where animals were kept in individual pen. Forty Ile de France lambs male non-castrated wereused in in vivo trial. To obtain rumen fluid, that was used in in vitro trial, four Santa Inês lambs with rumen cannula wereused. The treatments evaluated were four diets: Control diet: roughage + concentrate; PM20: roughage + concentrate withpeanut meal (PM) at 20% of DM; CG25: roughage + concentrate with crude glycerin (CG) at 25% of DM; and PMCG:roughage + concentrate with PM at 10% of DM and CG at 12.5% of DM. The roughage:concentrate ratio was 40:60 for allthese diets. The parameters of the in vitro and in vivo experiments used were completely randomized with four treatments.When significant, the means between treatments were compared using Tukey test (P < 0.05). There was no effect of coproduct inclusion on intake, except ether extract and neutral detergent fiber, which were higher for PM20 compared withCG25 diet. Apparent digestibility of dry matter (79.87%) and some nutrients (organic matter, crude protein, and neutraldetergent fiber)...

Utilização de coprodutos do biodiesel na alimentação de aves / Use of biodiesel coproducts in the poultry feeding

A nutrição animal tem evoluído ao longo dos anos, principalmente após a evolução nas técnicas de processamento dos alimentos. A partir do conhecimento da composição dos alimentos foi possível determinar o valor energético dos mesmos, que contribuiu para melhorias no sistema de produção, uma vez que as informações obtidas auxiliam nas formulações de rações, visando a produção animal e economia mais eficiente. Contudo, os grãos (milho e soja) tradicionalmente utilizados para compor as dietas sofrem com as variações de preço, por isso busca-se ampliar e diversificar através de pesquisas novas fontes energéticas que do ponto de vista nutricional possam contribuir positivamente para o desempenho dos animais e econômico com a redução dos custos com a alimentação. A glicerina e o glicerol, surge como um ingrediente alternativo por possuir alto valor energético, podendo ser utilizado como fonte de energia na alimentação de aves, sem prejudicar o desempenho e características de carcaça. No entanto, para que a sua utilização seja realizada, conhecer a sua composição físico-químicos se torna essencial, uma vez que este ingrediente se encontra disponível no mercado e não apresenta valores nutricionais padronizados, podendo influenciar nos resultados zootécnicos. Diante do exposto, objetivou-se com este trabalho dissertar através de revisão de literatura sobre o uso de coprodutos do biodiesel como fonte energética em dietas para aves sobre as características de desempenho e qualidade de carcaça.

Animal nutrition has evolved over the years, especially after the evolution of food processing techniques. With knowledge of the composition of the food it was possible to determine the energy value of the food, which contributed to improvements in the production system, since the information obtained helps feed formulations, aiming at more efficient animal production and economy. However, the grains (maize and soybean)traditionally used to compose diets suffer from price variations, so we have sought to expand and diversify through research new energy sources that from the nutritional point of view can contribute positively to the performance of animals and economically with the reduction of food costs. Glycerin and glycerol appear as an alternative ingredient because it has high energy value and can be used as a source of energy in poultry feed, without impairing performance and carcass characteristics. However, for its use to be carried out, knowing its physical-chemical composition becomes essential, since this ingredient is available on the market and does not present standardized nutritional values, which may influence the zootechnical results. Hence, the objective of this work is to present a literature review on the use of biodiesel co-products as an energy source in poultry diets about the performance and carcass quality characteristics.

Utilização de coprodutos do biodiesel na alimentação de aves / Use of biodiesel coproducts in the poultry feeding

A nutrição animal tem evoluído ao longo dos anos, principalmente após a evolução nas técnicas de processamento dos alimentos. A partir do conhecimento da composição dos alimentos foi possível determinar o valor energético dos mesmos, que contribuiu para melhorias no sistema de produção, uma vez que as informações obtidas auxiliam nas formulações de rações, visando a produção animal e economia mais eficiente. Contudo, os grãos (milho e soja) tradicionalmente utilizados para compor as dietas sofrem com as variações de preço, por isso busca-se ampliar e diversificar através de pesquisas novas fontes energéticas que do ponto de vista nutricional possam contribuir positivamente para o desempenho dos animais e econômico com a redução dos custos com a alimentação. A glicerina e o glicerol, surge como um ingrediente alternativo por possuir alto valor energético, podendo ser utilizado como fonte de energia na alimentação de aves, sem prejudicar o desempenho e características de carcaça. No entanto, para que a sua utilização seja realizada, conhecer a sua composição físico-químicos se torna essencial, uma vez que este ingrediente se encontra disponível no mercado e não apresenta valores nutricionais padronizados, podendo influenciar nos resultados zootécnicos. Diante do exposto, objetivou-se com este trabalho dissertar através de revisão de literatura sobre o uso de coprodutos do biodiesel como fonte energética em dietas para aves sobre as características de desempenho e qualidade de carcaça.(AU)

Animal nutrition has evolved over the years, especially after the evolution of food processing techniques. With knowledge of the composition of the food it was possible to determine the energy value of the food, which contributed to improvements in the production system, since the information obtained helps feed formulations, aiming at more efficient animal production and economy. However, the grains (maize and soybean)traditionally used to compose diets suffer from price variations, so we have sought to expand and diversify through research new energy sources that from the nutritional point of view can contribute positively to the performance of animals and economically with the reduction of food costs. Glycerin and glycerol appear as an alternative ingredient because it has high energy value and can be used as a source of energy in poultry feed, without impairing performance and carcass characteristics. However, for its use to be carried out, knowing its physical-chemical composition becomes essential, since this ingredient is available on the market and does not present standardized nutritional values, which may influence the zootechnical results. Hence, the objective of this work is to present a literature review on the use of biodiesel co-products as an energy source in poultry diets about the performance and carcass quality characteristics.(AU)

Energia metabolizável e aminoácidos digestíveis de coprodutos de trigo e de farinhas de origem animal em dietas para frangos de corte / [Metabolizable energy and digestible amino acids of wheat co-products and animal origin meals in broiler diets]

The objective of this study was to determine the metabolizable energy, standardized ileal amino acid digestibility (SIAAD) and digestible amino acid values of wheat meal (WM), wheat gluten meal (WGM), meat bone meal (MBM) and poultry-by-product meal (PPM) in diets for broilers. In the first experiment, the total excreta collection method was used, with 180 chicks with 14 days, distributed in a completely randomized design, with 4 feeds + reference diet with 6 replicates and 6 broilers each. In the second experiment, 180 broilers from 24 to 28 days old were used, distributed in a completely randomized design, with 4 feeds + one protein free diet with 6 replicates and 6 broilers each. The AMEn values of WM, WGM, MBM and PPM were 3052, 3174, 1556 and 3200 (kcal/kg), respectively. The average SIAAD of the essential and non-essential amino acids of WM, WGM, MBM and PPM were 74.21, 97.50, 69.28 and 83.08; 76.32, 96.42, 63.93 and 77.66, respectively. The values of the main digestible limiting amino acids were 0.18, 1.42, 0.41 and 0.94 for methionine; 0.20, 1.48, 1.73 and 2.73 for lysine; 0.23, 1.67, 0.72 and 1.89 for threonine; and 0.35, 3.22, 1.38 and 2.47 for valine, respectively for the feedstuffs in study.(AU)