Resumo
Salt stress and heavy metal are instigating hazard to crops, menace to agricultural practices. Single and combined stresses affecting adversely to the growth and metabolism of plants. To explore salt and heavy metal resistant plant lines as phytoremediants is a need of time. Physiological responses are main adaptive responses of the plants towards stresses. This response varies with species and ecotype as well as type and level of stress. Two cucurbit weeds from two ecotypes were selected to evaluate their physiological adaptations against independent and combined stresses of various levels of salt (NaCl) and heavy metal (NiCl2). Various physiological parameters like water potential, osmotic potential, pressure potential, CO2 assimilation rate, stomatal conductance, chlorophyll a and b, carotenoids, and production of adaptive chemicals like SOD, CAT, proteins, sugars and proline were studied. Citrullus colocynthis showed more adaptive response than Cucumis melo agrestis and desert ecotype was more successful than agricultural ecotype against stresses.
O estresse salino e os metais pesados são um perigo instigante para as plantações e uma ameaça para as práticas agrícolas. Estresses simples e combinados afetam adversamente o crescimento e o metabolismo das plantas. Explorar linhagens de plantas resistentes a sais e metais pesados como fitorremediantes é uma necessidade de tempo. As respostas fisiológicas são as principais respostas adaptativas das plantas aos estresses. Essas respostas variam de acordo com a espécie e o ecótipo, bem como com o tipo e o nível de estresse. Duas plantas daninhas cucurbitáceas de dois ecótipos foram selecionadas para avaliar suas adaptações fisiológicas frente a estresses independentes e combinados de vários níveis de sal (NaCl) e metal pesado (NiCl2). Vários parâmetros fisiológicos, como potencial hídrico, potencial osmótico, potencial de pressão, taxa de assimilação de CO2, condutância estomática, clorofila a e b, carotenoides e produção de produtos químicos adaptativos, por exemplo, SOD, CAT, proteínas, açúcares e prolina, foram estudados. Citrullus colocynthis apresentou resposta mais adaptativa do que Cucumis melo agrestis e o ecótipo deserto foi mais bem-sucedido do que o ecótipo agrícola contra estresses.
Assuntos
Biodegradação Ambiental , Metais Pesados/efeitos adversos , Citrullus , Controle de Plantas Daninhas , Estresse SalinoResumo
Abstract The most common form of psycho-social dysfunction is anxiety with depression being related closely without any age bar. They are present with combined state of sadness, confusion, stress, fear etc. Glyoxalase system contains enzyme named glyoxalase 1 (GLO1).It is a metabolic pathway which detoxifies alpha-oxo-aldehydes, particularly methylglyoxal (MG). Methylglyoxal is mainly made by the breakdown of the glycolytic intermediates, glyceraldehyde-3-phosphates and dihydroxyacetone phosphate. Glyoxylase-1 expression is also related with anxiety behavior. A casual role or GLO-1 in anxiety behavior by using viral vectors for over expression in the anterior cingulate cortex was found and it was found that local GLO-1 over expression increased anxiety behavior. The present study deals with the molecular mechanism of protective activity of eugenol against anxiolytic disorder. A pre-clinical animal study was performed on 42 BALB/c mice. Animals were given stress through conventional restrain model. The mRNA expression of GLO-1 was analyzed by real time RT-PCR. Moreover, the GLO-1 protein expression was also examined by immunohistochemistry in whole brain and mean density was calculated. The mRNA and protein expressions were found to be increased in animals given anxiety as compared to the normal control. Whereas, the expressions were decreased in the animals treated with eugenol and its liposome-based nanocarriers in a dose dependent manner. However, the results were better in animals treated with nanocarriers as compared to the compound alone. It is concluded that the eugenol and its liposome-based nanocarriers exert anxiolytic activity by down-regulating GLO-1 protein expression in mice.
Resumo A forma mais comum de disfunção psicossocial é a ansiedade intimamente relacionada com a depressão, sem qualquer barreira de idade. Elas estão presentes em um estado combinado de tristeza, confusão, estresse, medo etc. O sistema de glioxalase contém uma enzima chamada glioxalase 1 (GLO1). É uma via metabólica que desintoxica alfa-oxo-aldeídos, particularmente metilglioxal (MG). O metilglioxal é produzido principalmente pela quebra dos intermediários glicolíticos, gliceraldeído-3-fosfatos e fosfato de diidroxiacetona. A expressão da glioxalase 1 também está relacionada ao comportamento de ansiedade. Um papel casual ou GLO1 no comportamento de ansiedade usando vetores virais para superexpressão no córtex cingulado anterior foi encontrado e descobriu-se que a superexpressão local de GLO1 aumentava o comportamento de ansiedade. O presente estudo trata do mecanismo molecular da atividade protetora do eugenol contra o transtorno ansiolítico. Um estudo pré-clínico em animais foi realizado em 42 camundongos BALB / c. Os animais foram submetidos ao estresse por meio do modelo de contenção convencional. A expressão de mRNA de GLO1 foi analisada por RT-PCR em tempo real. Além disso, a expressão da proteína GLO1 também foi examinada por imuno-histoquímica em todo o cérebro e a densidade média foi calculada. Verificou-se que as expressões de mRNA e proteínas estavam aumentadas em animais que receberam ansiedade em comparação com o controle normal. Considerando que as expressões foram diminuídas nos animais tratados com eugenol e seus nanocarreadores baseados em lipossomas de forma dependente da dose. No entanto, os resultados foram melhores em animais tratados com nanocarreadores em comparação com o composto sozinho. Conclui-se que o eugenol e seus nanocarreadores baseados em lipossomas exercem atividade ansiolítica por regulação negativa da expressão da proteína GLO1 em camundongos.
Resumo
The most common form of psycho-social dysfunction is anxiety with depression being related closely without any age bar. They are present with combined state of sadness, confusion, stress, fear etc. Glyoxalase system contains enzyme named glyoxalase 1 (GLO1).It is a metabolic pathway which detoxifies alpha-oxo-aldehydes, particularly methylglyoxal (MG). Methylglyoxal is mainly made by the breakdown of the glycolytic intermediates, glyceraldehyde-3-phosphates and dihydroxyacetone phosphate. Glyoxylase-1 expression is also related with anxiety behavior. A casual role or GLO-1 in anxiety behavior by using viral vectors for over expression in the anterior cingulate cortex was found and it was found that local GLO-1 over expression increased anxiety behavior. The present study deals with the molecular mechanism of protective activity of eugenol against anxiolytic disorder. A pre-clinical animal study was performed on 42 BALB/c mice. Animals were given stress through conventional restrain model. The mRNA expression of GLO-1 was analyzed by real time RT-PCR. Moreover, the GLO-1 protein expression was also examined by immunohistochemistry in whole brain and mean density was calculated. The mRNA and protein expressions were found to be increased in animals given anxiety as compared to the normal control. Whereas, the expressions were decreased in the animals treated with eugenol and its liposome-based nanocarriers in a dose dependent manner. However, the results were better in animals treated with nanocarriers as compared to the compound alone. It is concluded that the eugenol and its liposome-based nanocarriers exert anxiolytic activity by down-regulating GLO-1 protein expression in mice.
A forma mais comum de disfunção psicossocial é a ansiedade intimamente relacionada com a depressão, sem qualquer barreira de idade. Elas estão presentes em um estado combinado de tristeza, confusão, estresse, medo etc. O sistema de glioxalase contém uma enzima chamada glioxalase 1 (GLO1). É uma via metabólica que desintoxica alfa-oxo-aldeídos, particularmente metilglioxal (MG). O metilglioxal é produzido principalmente pela quebra dos intermediários glicolíticos, gliceraldeído-3-fosfatos e fosfato de diidroxiacetona. A expressão da glioxalase 1 também está relacionada ao comportamento de ansiedade. Um papel casual ou GLO1 no comportamento de ansiedade usando vetores virais para superexpressão no córtex cingulado anterior foi encontrado e descobriu-se que a superexpressão local de GLO1 aumentava o comportamento de ansiedade. O presente estudo trata do mecanismo molecular da atividade protetora do eugenol contra o transtorno ansiolítico. Um estudo pré-clínico em animais foi realizado em 42 camundongos BALB / c. Os animais foram submetidos ao estresse por meio do modelo de contenção convencional. A expressão de mRNA de GLO1 foi analisada por RT-PCR em tempo real. Além disso, a expressão da proteína GLO1 também foi examinada por imuno-histoquímica em todo o cérebro e a densidade média foi calculada. Verificou-se que as expressões de mRNA e proteínas estavam aumentadas em animais que receberam ansiedade em comparação com o controle normal. Considerando que as expressões foram diminuídas nos animais tratados com eugenol e seus nanocarreadores baseados em lipossomas de forma dependente da dose. No entanto, os resultados foram melhores em animais tratados com nanocarreadores em comparação com o composto sozinho. Conclui-se que o eugenol e seus nanocarreadores baseados em lipossomas exercem atividade ansiolítica por regulação negativa da expressão da proteína GLO1 em camundongos.
Assuntos
Masculino , Animais , Camundongos , Ansiedade/tratamento farmacológico , Eugenol/administração & dosagem , Lactoilglutationa LiaseResumo
The most common form of psycho-social dysfunction is anxiety with depression being related closely without any age bar. They are present with combined state of sadness, confusion, stress, fear etc. Glyoxalase system contains enzyme named glyoxalase 1 (GLO1).It is a metabolic pathway which detoxifies alpha-oxo-aldehydes, particularly methylglyoxal (MG). Methylglyoxal is mainly made by the breakdown of the glycolytic intermediates, glyceraldehyde-3-phosphates and dihydroxyacetone phosphate. Glyoxylase-1 expression is also related with anxiety behavior. A casual role or GLO-1 in anxiety behavior by using viral vectors for over expression in the anterior cingulate cortex was found and it was found that local GLO-1 over expression increased anxiety behavior. The present study deals with the molecular mechanism of protective activity of eugenol against anxiolytic disorder. A pre-clinical animal study was performed on 42 BALB/c mice. Animals were given stress through conventional restrain model. The mRNA expression of GLO-1 was analyzed by real time RT-PCR. Moreover, the GLO-1 protein expression was also examined by immunohistochemistry in whole brain and mean density was calculated. The mRNA and protein expressions were found to be increased in animals given anxiety as compared to the normal control. Whereas, the expressions were decreased in the animals treated with eugenol and its liposome-based nanocarriers in a dose dependent manner. However, the results were better in animals treated with nanocarriers as compared to the compound alone. It is concluded that the eugenol and its liposome-based nanocarriers exert anxiolytic activity by down-regulating GLO-1 protein expression in mice.(AU)
A forma mais comum de disfunção psicossocial é a ansiedade intimamente relacionada com a depressão, sem qualquer barreira de idade. Elas estão presentes em um estado combinado de tristeza, confusão, estresse, medo etc. O sistema de glioxalase contém uma enzima chamada glioxalase 1 (GLO1). É uma via metabólica que desintoxica alfa-oxo-aldeídos, particularmente metilglioxal (MG). O metilglioxal é produzido principalmente pela quebra dos intermediários glicolíticos, gliceraldeído-3-fosfatos e fosfato de diidroxiacetona. A expressão da glioxalase 1 também está relacionada ao comportamento de ansiedade. Um papel casual ou GLO1 no comportamento de ansiedade usando vetores virais para superexpressão no córtex cingulado anterior foi encontrado e descobriu-se que a superexpressão local de GLO1 aumentava o comportamento de ansiedade. O presente estudo trata do mecanismo molecular da atividade protetora do eugenol contra o transtorno ansiolítico. Um estudo pré-clínico em animais foi realizado em 42 camundongos BALB / c. Os animais foram submetidos ao estresse por meio do modelo de contenção convencional. A expressão de mRNA de GLO1 foi analisada por RT-PCR em tempo real. Além disso, a expressão da proteína GLO1 também foi examinada por imuno-histoquímica em todo o cérebro e a densidade média foi calculada. Verificou-se que as expressões de mRNA e proteínas estavam aumentadas em animais que receberam ansiedade em comparação com o controle normal. Considerando que as expressões foram diminuídas nos animais tratados com eugenol e seus nanocarreadores baseados em lipossomas de forma dependente da dose. No entanto, os resultados foram melhores em animais tratados com nanocarreadores em comparação com o composto sozinho. Conclui-se que o eugenol e seus nanocarreadores baseados em lipossomas exercem atividade ansiolítica por regulação negativa da expressão da proteína GLO1 em camundongos.(AU)
Assuntos
Animais , Masculino , Camundongos , Ansiedade/tratamento farmacológico , Eugenol/administração & dosagem , Lactoilglutationa LiaseResumo
Background: Ozone therapy (OT) is a type of treatment that uses ozone gas, which has high oxidizing power. OT has been utilized for various diseases and to accelerate the wound healing process and reduce inflammation. Additionally, OT is a low-cost treatment recommended for patients that can be easily restrained. There are several methods of ozone administration, including topical use through bagging, cupping, or rectal insufflation, in addition to ozonated autohemotherapy. Based on the benefits of OT and the scarcity of reports describing its effects on white-eared opossums (Didelphis albiventris), we report on the use of ozone in the treatment of a severe facial wound in a white-eared opossum. Case: An adult female white-eared opossum weighing 1.6 kg was rescued from a fire. The patient was pregnant, dehydrated, hypothermic and had an extensive burn wound in the facial region. Due to the stress of captivity and its aggressive behavior, the animal needed to be chemically restrained with dexmedetomidine and butorphanol. Following sedation, the cephalic vein was catheterized and intravenous fluid therapy with ringer lactate was started to treat dehydration. Next, enrofloxacin, meloxicam, and tramadol hydrochloride were administered. Body rewarming was performed with thermal bags. The microbial load of the wound was reduced with chlorhexidine degerming solution followed by washing with saline solution. A thin layer of silver sulfadiazine cream was applied to the wound area. Over the following days the patient was treated with OT using the cupping technique combined with ozonated sunflower oil. After the first applications there was an improvement in the appearance of the wound, which initially had a purulent aspect. After 20 days of treatment the wound showed good granulation and there were no signs of infection, and at 45 days of treatment it was completely healed. The patient was then released with her baby. Discussion: Ozone therapy was included in the patient's treatment plan to enhance the tissue repair process in order to reintroduce the animal to the wild in the shortest possible time. The strategy was successful in improving healing and reducing tissue inflammation and infection. Ozone is known to cause the inactivation of microorganisms as a result of the oxidation of phospholipids and lipoproteins present in the cell membrane of bacteria and to interrupt viral replication. Ozone helps in the perfusion of injured tissues, contributing to the entry of humoral components, which control inflammation. Moreover, ozone contributes to increase supply of oxygen and nutrients to tissues and to stimulate increased production of fibrinogen and formation of granulation tissue. Ozone increases the enzymatic antioxidant response, while also playing a role in immunoregulation. Ozone can be used in several ways and via various methods with potential routes including intravenous, urethral, intramuscular, subcutaneous, or intra-articular, in addition to rectal and intramammary insufflation. Immunomodulatory, anti-inflammatory, analgesic, and antiseptic effects can be achieved with different doses and concentrations, which enables its application in different types of pathologies. Because of the aggressive behavior of the animal, in the current case we opted for the cupping technique combined with ozonated oil, both techniques which can be administered easily with only physical restraint of the patient. Cupping allows for the provision of a higher concentration of ozone gas locally whereas the ozonated oil maintains the effect of the gas for slightly longer, enhancing its effect and promoting tissue repair. The present report showed that OT is an effective alternative for the treatment of burn wounds in opossums, without causing adverse effects to the patient or her baby.
Assuntos
Animais , Feminino , Cicatrização , Didelphis/lesões , Traumatismos Faciais/veterinária , Ozonioterapia , Animais Selvagens/lesõesResumo
Abstract The most common form of psycho-social dysfunction is anxiety with depression being related closely without any age bar. They are present with combined state of sadness, confusion, stress, fear etc. Glyoxalase system contains enzyme named glyoxalase 1 (GLO1).It is a metabolic pathway which detoxifies alpha-oxo-aldehydes, particularly methylglyoxal (MG). Methylglyoxal is mainly made by the breakdown of the glycolytic intermediates, glyceraldehyde-3-phosphates and dihydroxyacetone phosphate. Glyoxylase-1 expression is also related with anxiety behavior. A casual role or GLO-1 in anxiety behavior by using viral vectors for over expression in the anterior cingulate cortex was found and it was found that local GLO-1 over expression increased anxiety behavior. The present study deals with the molecular mechanism of protective activity of eugenol against anxiolytic disorder. A pre-clinical animal study was performed on 42 BALB/c mice. Animals were given stress through conventional restrain model. The mRNA expression of GLO-1 was analyzed by real time RT-PCR. Moreover, the GLO-1 protein expression was also examined by immunohistochemistry in whole brain and mean density was calculated. The mRNA and protein expressions were found to be increased in animals given anxiety as compared to the normal control. Whereas, the expressions were decreased in the animals treated with eugenol and its liposome-based nanocarriers in a dose dependent manner. However, the results were better in animals treated with nanocarriers as compared to the compound alone. It is concluded that the eugenol and its liposome-based nanocarriers exert anxiolytic activity by down-regulating GLO-1 protein expression in mice.
Resumo A forma mais comum de disfunção psicossocial é a ansiedade intimamente relacionada com a depressão, sem qualquer barreira de idade. Elas estão presentes em um estado combinado de tristeza, confusão, estresse, medo etc. O sistema de glioxalase contém uma enzima chamada glioxalase 1 (GLO1). É uma via metabólica que desintoxica alfa-oxo-aldeídos, particularmente metilglioxal (MG). O metilglioxal é produzido principalmente pela quebra dos intermediários glicolíticos, gliceraldeído-3-fosfatos e fosfato de diidroxiacetona. A expressão da glioxalase 1 também está relacionada ao comportamento de ansiedade. Um papel casual ou GLO1 no comportamento de ansiedade usando vetores virais para superexpressão no córtex cingulado anterior foi encontrado e descobriu-se que a superexpressão local de GLO1 aumentava o comportamento de ansiedade. O presente estudo trata do mecanismo molecular da atividade protetora do eugenol contra o transtorno ansiolítico. Um estudo pré-clínico em animais foi realizado em 42 camundongos BALB / c. Os animais foram submetidos ao estresse por meio do modelo de contenção convencional. A expressão de mRNA de GLO1 foi analisada por RT-PCR em tempo real. Além disso, a expressão da proteína GLO1 também foi examinada por imuno-histoquímica em todo o cérebro e a densidade média foi calculada. Verificou-se que as expressões de mRNA e proteínas estavam aumentadas em animais que receberam ansiedade em comparação com o controle normal. Considerando que as expressões foram diminuídas nos animais tratados com eugenol e seus nanocarreadores baseados em lipossomas de forma dependente da dose. No entanto, os resultados foram melhores em animais tratados com nanocarreadores em comparação com o composto sozinho. Conclui-se que o eugenol e seus nanocarreadores baseados em lipossomas exercem atividade ansiolítica por regulação negativa da expressão da proteína GLO1 em camundongos.
Assuntos
Animais , Coelhos , Eugenol/uso terapêutico , Eugenol/farmacologia , Lactoilglutationa Liase/antagonistas & inibidores , Ansiedade/tratamento farmacológico , Lipossomos , Camundongos Endogâmicos BALB CResumo
The Ziwuling black goat is an indigenously in China, their offspring are frequently affected by congenital cryptorchidism. The extracellular matrix (ECM) contains cytokines and growth factors that regulate the development of the testis, and component changes often result in pathological changes. Cryptorchidism is closely related to structural changes in ECM. In this study, the histochemical staining, immunohistochemical, immunofluorescence and Western blot combined with semi-quantitative analysis was used to describe the distribution of the important ECM components Collagen type IV (Col IV), laminin (LN)and heparan sulfate proteoglycans (HSPG) in the normal and cryptorchid testes of Ziwuling black goats. Results showed that: The histochemical staining showed that the dysplasia of seminiferous tubules and decreased number of Sertoli cells in cryptorchidism, as well as sparse collagen fiber. Meanwhile, the distribution of reticular fibers is relatively rich. Furthermore, the PAS and AB staining in the interstitial vessels and lamina propria of seminiferous tubules is weak. The immunohistochemical and immunofluorescence revealed that Col IV, LN was strongly expressed in Leydig, Sertoli cells of normal testes and moderately positive in the spermatogonia and spermatids, but HSPG was not expressed in the spermatogonia. However, cryptorchidism, the expression of Col IV, LN and HPSG in Leydig, Sertoli cells significantly decreased, as well as the expression of Col IV and LN in capillary endothelial cells, but HSPG was moderately expressed in spermatogonia. Based on these data, the underdevelopment of spermatogenic epithelium, decreased synthesis function of collagen fibers and Leydig cells develop usually in the cryptorchidism were shown to be closely related to the abnormal metabolism of Col IV and LN. The positive expressed of HSPG in the spermatogonia of cryptorchid testes is related to the compensatory development of spermatogonia.(AU)
Assuntos
Animais , Feminino , Ovulação/fisiologia , Ovinos/fisiologia , Alimentos Fortificados/efeitos adversos , Estresse Oxidativo/fisiologia , Folículo Ovariano/crescimento & desenvolvimento , Aditivos Alimentares/efeitos adversos , Glicerol/efeitos adversos , Fenômenos Fisiológicos da Nutrição AnimalResumo
The objective of this study was to elucidate the optimum protocol timing of thermal manipulation (TM) during embryogenesis, which underline genetic improvement of muscle thermotolerance acquisition. For the present study, 1,440 fertile eggs were divided randomly and equally into control (37.8 °C with 56% relative humidity) and four thermally manipulated groups (TM1, TM2, TM3, and TM4) subjected to 39 °C for 18 h with 65% relative humidity daily during different embryonic periods. Then, at day 35 post-hatch, all groups were subjected to thermal challenge at 43 °C for 6 h to identify the level of thermotolerance acquisition differences between them. Hsp70 mRNA expression was evaluated by using a relative quantitatively RT-qPCR. Single nucleotide polymorphisms sequence of the Hsp70 gene was evaluated by Sanger's sequencing method. Pectoral and thigh muscles samples were subjected to immunohistochemistry to detect Hsp70. Among TM conditions that were investigated, TM1 (39 °C for 18 h during embryonic days (ED) 711) induced a significant improvement in thermotolerance parameters (body temperature and T3 levels) during thermal challenge combined with an increase in the levels of Hsp70 mRNA and its protein with a high stability of nucleotide sequences in both pectoral and thigh muscles. The partial DNA sequence of Hsp70 gene in TM1 was reported, and nucleotide sequences were deposited in NCBI GenBank database with the accession numbers (MK852579) and (MK852580). Thigh muscle thermotolerance acquisition was higher than pectoral muscle during thermal challenge at 43 °C for 6 h. Thus, TM during ED711 may improve thermotolerance acquisition without adversely affecting performance.(AU)
Assuntos
Animais , Embrião de Galinha , Galinhas/fisiologia , Resposta ao Choque Térmico/genética , Desenvolvimento EmbrionárioResumo
In view of the need to increase genetic variability to obtain materials with a significant capacity to drought tolerance, this study conducted a cycle of a reciprocal recurrent selection of full-sib families of maize. To this end, 64 full-sib families of maize were evaluated in two environments according to their morpho-agronomic data in a randomized block design with two replicates. It were analyzed of Male flowering (MF); Female flowering (FF); Flowering interval (IF); days for flowering (DF); Plant height (PH); Ear height (EH); number of plants at the Stand (NPS); Number of broken plant (NBrP); Number of bedded plants (NBeP); Strawing (St); Ear length (EL); Ear diameter (ED); Ear number (EN); Prolificacy (Pr); Number of diseased ears (NDE); Number of ears attacked by pests (NEP); Ear weight (EW); Yield (YIE) and Total Chlorophyll Index (TCI). The analysis of variance was performed by the F test at 5% significance level, and also the evaluation of genetic parameters. Regarding morpho-agronomic data, the analysis of variance and the analysis of genetic parameters showed that there was no interaction genotype x environment with regard to the genetic variability among the families under study. Lastly, the final selection of the superior genotypes was made on the basis of the ranking of the 40 most productive families, from which, combined with the molecular data, the 30 most productive, most drought-tolerant, and most genetically diverse ones were selected to compose the next cycle of recurrent maize selection aiming water-stress tolerance.
Tendo em vista a necessidade de aumentar a variabilidade genética para obter materiais com significativa capacidade de tolerância à seca, este estudo conduziu um ciclo de seleção recorrente recíproca de famílias de irmãos completos de milho. Para tanto, 64 famílias de irmãos completos de milho foram avaliadas em dois ambientes de acordo com seus dados morfoagronômicos em um delineamento de blocos casualizados com duas repetições. Foram analisados o florescimento masculino (MF); florescimento feminino (FF); Intervalo de florescimento (IF); dias para florescimento (DF); Altura da planta (PH); Altura da espiga (EH); número de plantas na parcela (NPS); Número de planta quebrada (NBrP); Número de plantas com acamadas (NBeP); empalhamento (St); Comprimento da espiga (EL); Diâmetro da espiga (DE); Número de espigas (EN); Prolificidade (Pr); Número de espigas doentes (EQM); Número de espigas atacadas por pragas (NEP); Peso de espiga (EW); Rendimento de grãos (YIE) e Índice de clorofila total (TCI). A análise de variância foi realizada pelo teste F com nível de significância de 5% e também pela avaliação dos parâmetros genéticos. Em relação aos dados morfoagronômicos, a análise de variância e a análise dos parâmetros genéticos mostraram que não houve interação genótipo x ambiente no que diz respeito à variabilidade genética entre as famílias em estudo. Por fim, a seleção final dos genótipos superiores foi feita com base no ranking das 40 famílias mais produtivas, das quais, combinadas com os dados moleculares, foram selecionadas as 30 mais produtivas, mais tolerantes à seca e mais geneticamente diversificadas. para compor o próximo ciclo de seleção recorrente de milho visando tolerância ao estresse hídrico.
Assuntos
Variação Genética , Zea mays/crescimento & desenvolvimento , Secas , Melhoramento Vegetal/métodosResumo
The use of dogs in military work environments has always aroused great interest in the general population and determining the stress levels they go through is extremely important to maintain their welfare. The aim of this research was to evaluate if the work shifts in military working dogs leads to stress conditions and if this working influences on the reproductive performance and life quality. The study was conducted at the Military Police Kennel located at Campinas, Sao Paulo, Brazil. Eight male dogs of four different breeds (German Shepherd, Belgian Malinois Shepherd, Doberman, and Rottweiler) were evaluated during two different shifts: Working Shifts: animals working 12 hours a day with 2 hour-interval; and Control Shifts: animals that were on their day off (36 hours). Saliva samples were collected for cortisol analysis at the control and working shifts. The study was carried out over 60 days and analyzed behavior, physiology, and reproduction quality. Saliva samples, behavior observation of stereotyping, resting and moving activities and semen analysis were collected by digital stimulation (for combined second and third fractions). The salivary cortisol levels during the control and working shifts were between 0.361-0.438 and 0.312-0.592 µg/dL, respectively; the highest values were found at the end of working shifts. The animals were resting during most of the observation period, but few showed stereotypic behaviors. The testicular consistency was firm and semen parameters were within the normal values in German Shepherd, Belgian Malinois Shepherd, and Doberman dogs. However, Rottweiler dogs had a higher rate of sperm abnormalities, higher salivary cortisol levels, and more stereotypic behaviors. Nevertheless this work highlights the importance of further research relating reproduction and cortisol levels in military dogs.(AU)
Assuntos
Animais , Cães , Bem-Estar do Animal , Hidrocortisona , Análise do Sêmen , Técnicas de Observação do Comportamento , Comportamento Sexual AnimalResumo
Background: Polymyositis is a generalized inflammatory myopathy which can lead to rhabdomyolysis. This affection may have several origins, including degenerative, metabolic, autoimmune, infectious, inflammatory, ischemic, traumatic, by drug use, induced by toxins and also of idiopathic origin. Diagnosis is made with seric dosage, electrodiagnostic tests and muscle biopsy. Lesions in the rostral oblong medulla may affect the central vestibular system, and there may be signs such as opisthotonos, nystagmus, and strabismus. The aim of this report is to describe a case of a mixed breed dog with manifestation of polymyositis associated with brainstem signs of probable idiopathic origin. Case: A 5-year-old mixed breed male dog was attended with opisthotonos episodes for 2 days, and pelvic limbs extension and thoracic limbs flexion that lasted 10 to 20 min at intervals of approximately 1 h. The animal was anorexic and had also presented one episode of emesis. Upon neurological examination, ventromedial strabismus and Horner's syndrome was observed on the right side, besides vertical nystagmus, flaccid tetraparesis and absence of proprioception in the four limbs. Biochemical analyses revealed creatine kinase (CK) increased (2,433.9 UI/L - reference: 1.5-28.4 UI/L), and urinalysis showed dark color and presence of occult blood without, however, erythrocyturia. Electrocardiogram (ECG) showed QS wave and deviation of the electrical axis. Treatment with prednisolone (1 mg/kg, BID), phenobarbital (2 mg/kg, BID), maropitant citrate (1 mg/kg in 2 doses), and crystalloid fluid therapy (50 mL/kg/day) were prescribed. On the 4th day, the dog was more active and feeding without a tube, so it recommended keep the treatment at home. On the 10th day, the animal had proprioception present on the 4 limbs and normorexia. Biochemical analyses and urinalysis showed no alterations, but normochromic normochromic anemia with thrombocytopenia and leukocytosis by neutrophilia showed in blood count exam. PCR to Ehrlichia canis, Hepatozoon sp., and Babesia canis resulted negative. On the 15th day, blood count, biochemical analyses and urinalysis showed no alterations. Neurological examination revealed only positional vertical nystagmus. which remained as a sequel. Discussion: Polymyositis may be accompanied by rhabdomyolysis, characterized by acute muscle necrosis, increased CK and myoglobinuria. The animal had polymyositis of acute onset, with myoglobinuria and elevated CK values, whose presentation included myalgia and muscle weakness. In humans, polymyositis is accompanied by changes in electrocardiographic tracing without clinical alterations. In dogs, the first report that showed cardiac involvement was compatible with myocarditis. The changes in ECG in the present case was attributed to failure in myocardial electrical conduction. The patient also showed signs of brainstem and central vestibular system injuries. Stress myopathy, intoxication, snakebite, infectious, and metabolic diseases were discarded leading to a clinical suspicion as idiopathic origin. Similar to a published case, the patient of this report received symptomatic and supportive treatment, being discharged from the hospital 20 days after the onset of clinical signs. Thus, polymyositis may be accompanied by signs indicative of brainstem injury. Patients with rhabdomyolysis require intense monitoring due to the high risk of developing acute renal failure. Since no causative agent was identified, symptomatic treatment combined with the prevention of possible complications were fundamental for the maintenance of the animal's life.
Assuntos
Animais , Masculino , Cães , Polimiosite/terapia , Polimiosite/veterinária , Rabdomiólise/veterinária , Síndrome de Horner/veterinária , Mioglobinúria/veterináriaResumo
As ondas de calor têm despertado a preocupação quanto aos danos que ocasionam sobre os serviços ecossistêmicos, e combinadas com estiagens, o excesso de calor tem favorecido o aumento da mortalidade das árvores mundo afora. Partindo-se da hipótese em que a região sudoeste do Paraná, apresenta condições favoráveis à ocorrência de anomalias climáticas caracterizadas como ondas de calorde verão, este trabalho teve como objetivo geral, observar o comportamento da temperatura atmosférica no município de Dois Vizinhos, para o interstício entre 2018 a 2020, e em específico: i) analisar quais os intervalos dos horários de verão em Dois Vizinhos, a temperatura atmosférica se apresenta mais elevada;ii) verificar por meio do índice proposto de estresse florestal (forest-based stress index ou FBS), os eventos caracterizados como de onda de calor, entre os anos de 2018 a 2020; iii) discutir a viabilidade do índice FBS, tendo como critério, outros índices de monitoramento ambiental; iv) apresentar argumentos sobre aspectos ecofisiológicos e de conservação florestal. As conclusões foram que o período noturno entre 21h-03h de 2020, da estação de verão de Dois Vizinhos, foi significativamente mais quente, no triênio analisado. O horário entre 15h-21h de 2020 foi significativamente mais quente, entre as categorias horarias analisadas. Por meio do índice FBS de estresse florestal, verificou-se o total de 36 episódios caracterizados como de Ondas de Calor na localidade, inclusive noturnas, entre os anos de 2018 a 2020.
Heat waves have raised concerns about the damage they do to ecosystem services, and combined with droughts, excessive heat has favored the rise in tree mortality worldwide. Based on the hypothesis that the southwestern region of the state of Paraná presents suitable conditions for the occurrence of anomalousweather events characterized as summer heat waves, this study was conducted to observe air temperature patterns in the municipality of Dois Vizinhos between 2018 and 2020. Its specific objectives were to i) determine which periods of the day during the summer in Dois Vizinhos registered the highest air temperatures; ii) verify the events characterized as heat waves between the years 2018 and 2020 using the proposed forest-based stress index (FBS); iii) discuss the feasibility of the FBS index by making reference to other environmental monitoring indices; and iv) present arguments on aspects of ecophysiology and forest conservation. The results showed that the night period between 9 p.m. and 3 a.m. in Dois Vizinhos in summer 2020 was significantly hotter in the three years of the study. The interval between 3 p.m. and 9 p.m. in summer 2020 was significantly hotter among the hourly categories recorded. According to the FBS index, there were a total of 36 episodes characterized as Heat Waves in this region,including the nighttime.
Assuntos
Florestas , Monitoramento Ambiental/métodos , Transtornos de Estresse por Calor , TemperaturaResumo
As ondas de calor têm despertado a preocupação quanto aos danos que ocasionam sobre os serviços ecossistêmicos, e combinadas com estiagens, o excesso de calor tem favorecido o aumento da mortalidade das árvores mundo afora. Partindo-se da hipótese em que a região sudoeste do Paraná, apresenta condições favoráveis à ocorrência de anomalias climáticas caracterizadas como ondas de calorde verão, este trabalho teve como objetivo geral, observar o comportamento da temperatura atmosférica no município de Dois Vizinhos, para o interstício entre 2018 a 2020, e em específico: i) analisar quais os intervalos dos horários de verão em Dois Vizinhos, a temperatura atmosférica se apresenta mais elevada;ii) verificar por meio do índice proposto de estresse florestal (forest-based stress index ou FBS), os eventos caracterizados como de onda de calor, entre os anos de 2018 a 2020; iii) discutir a viabilidade do índice FBS, tendo como critério, outros índices de monitoramento ambiental; iv) apresentar argumentos sobre aspectos ecofisiológicos e de conservação florestal. As conclusões foram que o período noturno entre 21h-03h de 2020, da estação de verão de Dois Vizinhos, foi significativamente mais quente, no triênio analisado. O horário entre 15h-21h de 2020 foi significativamente mais quente, entre as categorias horarias analisadas. Por meio do índice FBS de estresse florestal, verificou-se o total de 36 episódios caracterizados como de Ondas de Calor na localidade, inclusive noturnas, entre os anos de 2018 a 2020.(AU)
Heat waves have raised concerns about the damage they do to ecosystem services, and combined with droughts, excessive heat has favored the rise in tree mortality worldwide. Based on the hypothesis that the southwestern region of the state of Paraná presents suitable conditions for the occurrence of anomalousweather events characterized as summer heat waves, this study was conducted to observe air temperature patterns in the municipality of Dois Vizinhos between 2018 and 2020. Its specific objectives were to i) determine which periods of the day during the summer in Dois Vizinhos registered the highest air temperatures; ii) verify the events characterized as heat waves between the years 2018 and 2020 using the proposed forest-based stress index (FBS); iii) discuss the feasibility of the FBS index by making reference to other environmental monitoring indices; and iv) present arguments on aspects of ecophysiology and forest conservation. The results showed that the night period between 9 p.m. and 3 a.m. in Dois Vizinhos in summer 2020 was significantly hotter in the three years of the study. The interval between 3 p.m. and 9 p.m. in summer 2020 was significantly hotter among the hourly categories recorded. According to the FBS index, there were a total of 36 episodes characterized as Heat Waves in this region,including the nighttime.(AU)
Assuntos
Florestas , Monitoramento Ambiental/métodos , Transtornos de Estresse por Calor , TemperaturaResumo
In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.(AU)
Assuntos
Animais , Masculino , Feminino , Gravidez , Ratos , Comportamento Animal , Transtornos Relacionados a Trauma e Fatores de Estresse/sangue , Prenhez/psicologia , Ratos WistarResumo
In the present work, we sought to determine the effects of an individual application of prenatal stress and postnatal stress and their combination on the anxious and locomotor behavior of first-time mother rats. Indeed, three batches of rats were exposed to three types of daily repeated stress: one batch exposed to prenatal restraint stress (SC): 01h a day from day 11 to day 19 of gestation, one batch exposed to postnatal mother prenatal stress (SS): 03h a day from the third to the fourteenth postnatal day (JPN), and one batch exposed to combined restraint stress: 01h from day 11 to day 19 of gestation followed by a postnatal mother separation of 03h a day from the third to fourteenth JPN (CSS); a fourth control batch (T) of female rats that did not undergo any type of stress was also part of our study. At postnatal days 15 and 20, the behavior of the female rats in the four batches was assessed using the Open field Test along with the Plus Maze, and at JPN 30, the female rats were decapitated, the blood of which was collected for plasma adrenocorticotropic hormone (ACTH) level evaluation. Our findings show that all studied behaviors were very highly affected relative to the results of the control batch after individually applying prenatal restraint stress and mother separation. We noticed an intensified anxious behavior in CSS with a significant increase in plasma levels of ACTH.
Assuntos
Masculino , Feminino , Animais , Gravidez , Ratos , Comportamento Animal , Prenhez/psicologia , Transtornos Relacionados a Trauma e Fatores de Estresse/sangue , Ratos WistarResumo
Water deficit and iron nutritional deficiency (iron chlorosis) are frequent environmental stresses affecting grapevine production in the Mediterranean region. The objectives of this work were (i) to study the combined effects of both stresses on aromatic profile of Tempranillo grapes, occurring simultaneously in the vineyard, and (ii) to evaluate the viability of foliar chlorophyll content at veraison (Chl) to early assess aromatic quality potential of grapes in those conditions. Twenty non-irrigated vineyard subzones (10 m × 10 m each), affected and non-affected by iron chlorosis, were monitored in Ribera del Duero Appellation of Origin (North-Central Spain) during two consecutive seasons. Factorial ANOVA was performed to study the effects of predawn leaf water potential and Chl measured at veraison on the must composition parameters and, specifically, on the concentrations of free and bound aromatic compounds. Water deficit tended to increase color intensity and extractable anthocyanin content of the grapes, whereas the incidence of iron deficiency enhanced total phenolic compound content within subzones with better water status. More water or iron stressed subzones restricted C6-alcohols contents than less stressed subzones. Without significant effects on vine vigor, yield or berry size, the incidence of iron chlorosis increased the concentrations of some specific terpenes, C13-norisoprenoids, volatile acids and volatile phenols. These results showed that low to moderate iron stress can have positive effects on grape aromatic quality, and demonstrated that Chl can be a useful tool in precision viticulture to map the aromatic potential within rainfed vineyards affected by iron chlorosis.
Assuntos
Anemia Hipocrômica , Deficiências de Ferro , Desidratação , Odorantes , Vitis/química , ClorofilaResumo
Water deficit and iron nutritional deficiency (iron chlorosis) are frequent environmental stresses affecting grapevine production in the Mediterranean region. The objectives of this work were (i) to study the combined effects of both stresses on aromatic profile of Tempranillo grapes, occurring simultaneously in the vineyard, and (ii) to evaluate the viability of foliar chlorophyll content at veraison (Chl) to early assess aromatic quality potential of grapes in those conditions. Twenty non-irrigated vineyard subzones (10 m × 10 m each), affected and non-affected by iron chlorosis, were monitored in Ribera del Duero Appellation of Origin (North-Central Spain) during two consecutive seasons. Factorial ANOVA was performed to study the effects of predawn leaf water potential and Chl measured at veraison on the must composition parameters and, specifically, on the concentrations of free and bound aromatic compounds. Water deficit tended to increase color intensity and extractable anthocyanin content of the grapes, whereas the incidence of iron deficiency enhanced total phenolic compound content within subzones with better water status. More water or iron stressed subzones restricted C6-alcohols contents than less stressed subzones. Without significant effects on vine vigor, yield or berry size, the incidence of iron chlorosis increased the concentrations of some specific terpenes, C13-norisoprenoids, volatile acids and volatile phenols. These results showed that low to moderate iron stress can have positive effects on grape aromatic quality, and demonstrated that Chl can be a useful tool in precision viticulture to map the aromatic potential within rainfed vineyards affected by iron chlorosis.(AU)
Assuntos
Vitis/química , Odorantes , Deficiências de Ferro , Anemia Hipocrômica , Desidratação , ClorofilaResumo
Background: The brown bear (Ursus arctos) is considered one of the largest terrestrial carnivores, native from temperate forest regions of North America, Europe and Asia. In Brazil, they are founded in captivity and their safe capture and immobilization are obtained with one effective anesthesia for management and surgical and diagnostic procedures. Some anesthetic protocols are described for these purposes, however, there is a lack for data on the anesthetic and adverse effects they have on bears when used. The aim of this case is to report the use and effects of the association of dexmedetomidine with tiletamine and zolazepam in the chemical containment of a captive adult brown bear. Case: A 33-year-old female brown bear, weighing 100 kg, belonging to the Zoobotanic Park of Teresina, Piauí, Brazil, was chemically immobilized for an incisional biopsy of a cutaneous nodule with 1.0 cm diameter in the right face region. The anesthetic protocol included 6 µg/kg of dexmedetomidine associated with 3 mg/kg of tiletamine and zolazepam, administered intramuscularly by dart into the gluteal region of the right pelvic limb. The animal showed moderate ataxia at 5 min and assumed sternal decubitus 7 min after anesthetic administration. The bear's degree of sedation was considered adequate and safe to perform the biopsy at 10 min after administration. Heart rate (47 ± 3 bpm), respiratory rate (17 ± 2 mpm) and rectal temperature (38.7 ± 0.1ºC) were monitored. The bear remained immobile and unconscious throughout the procedure, with intense muscle relaxation, bilateral eyeball rotation, absent lateral palpebral reflex and mild medial reflex and without nystagmus. Complementary sedative doses were not necessary. At the beginning of anesthetic recovery, the bear received 6 µg/kg of atipamezole, intramuscularly. After 25 min of administration of atipamezole, the animal showed signs of recovery in the level of consciousness and reactivity to external stimuli, and assumed the quadrupedal position at 60 min after reverser application. Discussion: Even in captivity, the bears behavior is unpredictable and attack can occur, causing trauma or death to people. For this, the chemical immobilization is important to keep safety of everyone. This procedure was performed using blowgunassisted darts thrown by a staff member who had experience in using this method, who darted accurately and effectively. The latency time observed after administration of the anesthetic protocol used is similar to reported in other studies with bears that also received intramuscular dexmedetomidine and tiletamine and zolazepam and showed intense muscle relaxation and immobility. The doses used contributed to the absence of bradycardia and hypoventilation and, performing the procedure in the morning, when the temperature is milder in the city, minimized the chance of hyperthermia and thermal stress in the animal, not requiring body cooling. The use of dexmedetomidine in chemical containment protocols for short and minimally invasive procedures allows the subsequent use of its antagonist, atipamezole, contributing to a shorter recovery time, return of the animal's degree of consciousness and lower incidence of ataxia after assuming a quadrupedal position. The anesthetic protocol used was considered efficient, providing a quick and gentle chemical containment, adequate anesthetic plan and good anesthetic recovery in an adult brown bear from captivity.
Assuntos
Animais , Feminino , Tiletamina/administração & dosagem , Ursidae , Zolazepam/administração & dosagem , Dexmedetomidina/administração & dosagem , Imobilização/veterinária , Biópsia/veterinária , Anestésicos Combinados/análise , Animais SelvagensResumo
A rúcula é uma cultura de rápido crescimento vegetativo e ciclo curto, de grande aceitação mundial. No Brasil, sua produção gera emprego e renda à agricultura familiar. Entretanto, o uso de água salina no cultivo dessa planta é um dos principais fatores que limitam a produção. Diante dessa conjuntura, objetivou-se nesse estudo avaliar o crescimento de rúcula cultivar Apreciatta cultivada sob condições de sombreamento e níveis de salinidade da água de irrigação. O experimento foi conduzido em casa de vegetação na área experimental do Centro de Ciências Agrárias da Universidade Federal de Alagoas, na cidade de Rio Largo, AL.Os tratamentos foram obtidos da combinação de duas condições de sombreamento (com sobreamento e sem sombreamento) e cinco níveis de condutividade elétrica da água de irrigação (0,5; 2,0; 3,5; 5,0 e 6,5 dS m-1). O delineamento experimental utilizado foi o inteiramente casualizado em esquema fatorial2 x 5. Combinados, os fatores resultaram em 10 tratamentos com quatro repetições e uma planta por parcela, totalizando 40 unidades experimentais. As plantas de rúcula foram avaliadas quanto ao número de folhas, altura de plantas, área foliar e massa seca da parte aérea. Níveis crescentes de salinidade da água de irrigação levam a redução do número de folhas, altura de plantas e massa seca da parte aérea de plantas de rúcula cultivar Apreciatta. Entretanto, a utilização de forma conjunta do sombreamento com a salinidade da água de irrigação até 1,4 dS m-1não afeta a área foliar das plantas de rúculas, sendo possível o cultivo sem que ocorra perdas consideráveis.
Arugula is a crop of fast vegetative growth and short cycle, widely accepted worldwide. In Brazil, its production generates jobs and income for family farming. However, the use of saline water in the cultivation of this plant is one of the main factors that limit production. Given this situation, the objective of this study was to evaluate the growth of Apreciatta arugula cultivated under conditions of shading and salinity levels of irrigation water. The experiment was conducted in a greenhouse in the experimental area of the Agricultural Sciences Center of the Federal University of Alagoas, in the city of Rio Largo, AL. The treatments were obtained from the combination of two shading conditions (with shading and without shading) and five levels of electrical conductivity of the irrigation water (0.5; 2.0; 3.5; 5.0 and 6.5 dS m-1). The experimental design used was completely randomized in a 2 x 5 factorial scheme. Combined, the factors resulted in 10 treatments with four replications and one plant per plot, totaling 40 experimental units. Arugula plants were evaluated for leaf number, plant height, leaf area and dry matter of the aerial part. Increasing levels of salinity in the irrigation water, lead to a reduction in the number of leaves, plant height and dry mass of the aerial part of arugula plants cultivar Apreciatta. However, the use of shading together with the salinity of irrigation water up to 1.4 dS m-1does not affect the leaf area of arugula plants, being possible to cultivate without considerable losses.
Assuntos
Brassicaceae/crescimento & desenvolvimento , Estresse Salino , Irrigação Agrícola , SalinidadeResumo
A rúcula é uma cultura de rápido crescimento vegetativo e ciclo curto, de grande aceitação mundial. No Brasil, sua produção gera emprego e renda à agricultura familiar. Entretanto, o uso de água salina no cultivo dessa planta é um dos principais fatores que limitam a produção. Diante dessa conjuntura, objetivou-se nesse estudo avaliar o crescimento de rúcula cultivar Apreciatta cultivada sob condições de sombreamento e níveis de salinidade da água de irrigação. O experimento foi conduzido em casa de vegetação na área experimental do Centro de Ciências Agrárias da Universidade Federal de Alagoas, na cidade de Rio Largo, AL.Os tratamentos foram obtidos da combinação de duas condições de sombreamento (com sobreamento e sem sombreamento) e cinco níveis de condutividade elétrica da água de irrigação (0,5; 2,0; 3,5; 5,0 e 6,5 dS m-1). O delineamento experimental utilizado foi o inteiramente casualizado em esquema fatorial2 x 5. Combinados, os fatores resultaram em 10 tratamentos com quatro repetições e uma planta por parcela, totalizando 40 unidades experimentais. As plantas de rúcula foram avaliadas quanto ao número de folhas, altura de plantas, área foliar e massa seca da parte aérea. Níveis crescentes de salinidade da água de irrigação levam a redução do número de folhas, altura de plantas e massa seca da parte aérea de plantas de rúcula cultivar Apreciatta. Entretanto, a utilização de forma conjunta do sombreamento com a salinidade da água de irrigação até 1,4 dS m-1não afeta a área foliar das plantas de rúculas, sendo possível o cultivo sem que ocorra perdas consideráveis.(AU)
Arugula is a crop of fast vegetative growth and short cycle, widely accepted worldwide. In Brazil, its production generates jobs and income for family farming. However, the use of saline water in the cultivation of this plant is one of the main factors that limit production. Given this situation, the objective of this study was to evaluate the growth of Apreciatta arugula cultivated under conditions of shading and salinity levels of irrigation water. The experiment was conducted in a greenhouse in the experimental area of the Agricultural Sciences Center of the Federal University of Alagoas, in the city of Rio Largo, AL. The treatments were obtained from the combination of two shading conditions (with shading and without shading) and five levels of electrical conductivity of the irrigation water (0.5; 2.0; 3.5; 5.0 and 6.5 dS m-1). The experimental design used was completely randomized in a 2 x 5 factorial scheme. Combined, the factors resulted in 10 treatments with four replications and one plant per plot, totaling 40 experimental units. Arugula plants were evaluated for leaf number, plant height, leaf area and dry matter of the aerial part. Increasing levels of salinity in the irrigation water, lead to a reduction in the number of leaves, plant height and dry mass of the aerial part of arugula plants cultivar Apreciatta. However, the use of shading together with the salinity of irrigation water up to 1.4 dS m-1does not affect the leaf area of arugula plants, being possible to cultivate without considerable losses.(AU)