Influence of urbanization on stream fish assemblages in three microbasins in the Upper Paraná River Basin / Influência da urbanização em assembleias de peixes de riachos em três microbacias na bacia do Alto Rio Paraná

The colonization pattern of fish assemblages in streams is often studied in the context of environmental filters. On the other hand, when fish assemblages are subjected to anthropogenic effects, variables associated with environmental quality assume more importance. Therefore, this work evaluated the richness and composition of fish from streams sampled at different urbanization levels, aiming to determine any direct effects on the structure of fish assemblages. To accomplish this, samples were collected from 2003 to 2011 at 31 sites distributed among 3 microbasins in the Rio Ivinhema Basin, Alto Rio Paraná. Based on environmental variables, physicochemical of the water and analysis of the use and occupation of the soil, the microbasins were classified into different urbanization levels (low, medium and high). A total of 4,320 individuals were sampled, out of which 57 fish species were recorded. Sampled sites with medium urbanization level presented higher richness compared to sampled sites with high urbanization level which presented lower richness. Species richness in these sites was explained mainly by water temperature and water velocity. Results confirmed that urbanization does directly affect environmental integrity, which, in turn, can lead to the homogenization of stream assemblages.

Estudos sobre assembleias de peixes em riachos enfatizam o papel dos filtros ambientais associados ao padrão de colonização das assembleias, por outro lado, quando a assembleia está sujeita aos efeitos antropogênicos, as variáveis ambientais associadas à qualidade ambiental assumem maior importância. Dessa forma, o objetivo deste estudo foi analisar a riqueza e a composição de peixes de riachos amostrados em diferentes níveis de urbanização, buscando evidenciar se o efeito reflete diretamente na estrutura das assembleias. As amostragens foram realizadas em 31 locais distribuídos em três microbacias na bacia Rio Ivinhema, Alto Rio Paraná, no período de 2003 a 2011. Com base nas variáveis ambientais, físico-químicos da água e análise do uso e ocupação do solo as microbacias foram classificadas em diferentes níveis de urbanização (baixo, médio e alto). Foram amostrados um total 4.320 indivíduos, dos quais foram registradas 57 espécies de peixes. Os locais amostrados com influência média da urbanização apresentaram maior riqueza de espécies, entretanto, os locais amostrados com alta influência da urbanização apresentaram menor riqueza de espécies. Dessa forma, constatamos que a urbanização influencia diretamente na integridade ambiental, que pode levar a homogeneização das assembleias de riachos.

Influence of urbanization on stream fish assemblages in three microbasins in the Upper Paraná River Basin / Influência da urbanização em assembleias de peixes de riachos em três microbacias na bacia do Alto Rio Paraná

The colonization pattern of fish assemblages in streams is often studied in the context of environmental filters. On the other hand, when fish assemblages are subjected to anthropogenic effects, variables associated with environmental quality assume more importance. Therefore, this work evaluated the richness and composition of fish from streams sampled at different urbanization levels, aiming to determine any direct effects on the structure of fish assemblages. To accomplish this, samples were collected from 2003 to 2011 at 31 sites distributed among 3 microbasins in the Rio Ivinhema Basin, Alto Rio Paraná. Based on environmental variables, physicochemical of the water and analysis of the use and occupation of the soil, the microbasins were classified into different urbanization levels (low, medium and high). A total of 4,320 individuals were sampled, out of which 57 fish species were recorded. Sampled sites with medium urbanization level presented higher richness compared to sampled sites with high urbanization level which presented lower richness. Species richness in these sites was explained mainly by water temperature and water velocity. Results confirmed that urbanization does directly affect environmental integrity, which, in turn, can lead to the homogenization of stream assemblages.(AU)

Estudos sobre assembleias de peixes em riachos enfatizam o papel dos filtros ambientais associados ao padrão de colonização das assembleias, por outro lado, quando a assembleia está sujeita aos efeitos antropogênicos, as variáveis ambientais associadas à qualidade ambiental assumem maior importância. Dessa forma, o objetivo deste estudo foi analisar a riqueza e a composição de peixes de riachos amostrados em diferentes níveis de urbanização, buscando evidenciar se o efeito reflete diretamente na estrutura das assembleias. As amostragens foram realizadas em 31 locais distribuídos em três microbacias na bacia Rio Ivinhema, Alto Rio Paraná, no período de 2003 a 2011. Com base nas variáveis ambientais, físico-químicos da água e análise do uso e ocupação do solo as microbacias foram classificadas em diferentes níveis de urbanização (baixo, médio e alto). Foram amostrados um total 4.320 indivíduos, dos quais foram registradas 57 espécies de peixes. Os locais amostrados com influência média da urbanização apresentaram maior riqueza de espécies, entretanto, os locais amostrados com alta influência da urbanização apresentaram menor riqueza de espécies. Dessa forma, constatamos que a urbanização influencia diretamente na integridade ambiental, que pode levar a homogeneização das assembleias de riachos.(AU)

Aural hematomas in small ruminants - clinical features, surgical treatment and outcome

Background: Aural or auricular hematoma is an important and prevalent surgical condition in small animals practice, and commonly reported in companion pets. The condition is characterized by blood accumulation between the pinnae's dermal surface and the underlying perichondrium. In farm animals, most cases present surgical treatment with drainage of serosanguineous fluid from acute cases with clinical evolution ranging from 1 to 5 days. Therefore, the present work reports the clinical features, surgical treatment and outcome of aural hematomas in 3 small ruminants with a chronic evolution (7 to 20 days), detailing the post-surgical complications, such as wound infection and recurrence, and final cosmetic appearance of the pinnae. Cases: Upon physical examination, all animals were alert and presenting a good body condition score. Clinical alteration was restricted to a bilateral (Case 1) or unilateral (Cases 2 & 3) painless, and fluid-filled swelling, presenting doughy consistency masses within the fluid during pinna manipulation. The fluid-filled swellings were located on the concave (Case 1 - left ear & Case 2) and convex (Case 1 - right ear & Case 3) surface of the pinna. No primary pruritic disorders of the pinna were detected, and a diagnosis of traumatic aural hematoma was proposed. Due to the chronicity of the cases, surgical approach was advisable. The small ruminants underwent general anesthesia, and a linear incision over the skin overlying the hematoma was performed (Cases 1 & 2). After removal of blood and fibrin clots, the cavity was flushed and captonated size 0 nylon mattress sutures were performed on either side of the incision, in order to obliterate the dead space. Due to recurrence after 14 days, Case 2 was submitted to a modified surgical approach using a S-shaped incision combined to multiple drainage holes (MDH) using a disposable 6 mm biopsy punch. The same approach has performed in Case 3. Then, size 0 nylon mattress sutures followed by a tight protective pressure and absorbent bandage combined with an Elizabethan collar was applied. Postoperatively, Case 1 presented surgical site infection and the antibiotic was changed after bacterial culture and antibiogram results. Hospital discharge varied from 14 to 19 days' post-surgery. All animals presented some degree of ear retraction and a linear or S-shaped scar on the affected ear, but final cosmetic appearance was satisfactory to all owners. Discussion: Aural or auricular hematomas are a frequent disorder in dogs and cats clinical practice. In sheep, the few reported cases have been associated with pruritic disorders of the pinna, such as ticks infestation and sarcoptic mange. Whilst in goats, traumatic injuries, such as ear tagging and trauma from others goats, are considered the main cause, especially in breeds with pendulous ear. In our cases with chronic evolution (> 7 days), the most important clinical feature was the presence of doughy consistency masses within the fluid, suggesting matured blood and fibrin clots. A linear incision with captonated size 0 nylon mattress sutures was associated to post-surgical complications, such as wound infection and aural hematoma recurrence. Therefore, a S-shaped incision associated to creation of MDH, in order to achieve better drainage, was the chosen approach in the second surgery (Case 2) and on the subsequent patient (Case 3). To the best of our knowledge, this is the first report using the MDH approach in farm animals, that appears to be an effective treatment for chronic aural hematomas in small ruminants.

Pneumotórax secundário a bolha pulmonar em cão / Pneumothorax secondary to a pulmonary bullae in a dog

Background: Pulmonary bullae are thin-walled cavitary lesions within the subpleural parenchyma. They are a result of thedestruction, dilatation and coalescence of bordering alveoli and their rupture is the most common cause of pneumothoraxin dogs. Radiographic and CT imaging are excellent tools for identifying and quantifying pneumothorax. Surgical treatment is considered standard for treatment of pneumothorax consequential to pulmonary bullae. The aim of this report wasto describe a case of pneumothorax secondary to pulmonary bullae in a dog.Case: A 5-year-old male crossbreed dog, weighing 11.5 kg, was presented to the Uberaba’s Veterinary Hospital due to becoming easily tired in the previous 3 weeks, and its worsening in the last 2 days by presenting panting. The dog’s guardiandid not witness any traumas, but informed that the animal resided with other 14 dogs and also that it frequently collidedthe thorax against the door when it came down from the bed. Physical examination showed diaphragmatic breathing,inspiratory dyspnea and stridor lung sound. Thoracocentesis revealed presence of air in the pleural cavity and pneumothorax. Radiographic images confirmed this condition. The dog stayed in the hospital and chest drains were placed. Sincethe amount of sucked air did not reduce with time and due to the emergence of subcutaneous emphysema, the dog wentthrough exploratory thoracotomy that revealed impairment of the right caudal lung lobe, proceeding to lobectomy. Thedog stayed in the hospital with chest drains until the contents of the suctions reduced significantly. With the removal ofthe drains, the dog was sent home and had a full recovery. Histopathology of the impaired lung revealed pulmonary bullae.Discussion: The dog from this report presented clinical signs consistent with pneumothorax, such as dyspnea, diaphragmaticbreathing and exercise intolerance. Radiography of the chest region revealed...

A new species of Ancistrus (Siluriformes: Loricariidae) from Tapajós and Xingu basins, Brazil

A new Ancistrus species is described from Tapajós and Xingu river basins. It is distinguished from its congeners by the singular body color pattern, consisting of dark vermiculated stripes almost all over the body, and also by combination of features as a narrow head, large internostril distance, and absence of rows of enlarged odontodes on the lateral plates. In addition, the new species is distinguished from congeners that inhabit the rio Tapajós basin by the presence of a fully-developed adipose fin (vs. adipose fin absent in Ancistrus parecis and A. tombador, and vestigial adipose fin or absent in A. krenakarore). It differs from A. ranunculus, also from the rio Xingu, by the color pattern, smaller body size, smaller gill opening, and narrower cleithral width. The new taxon adds a new record to the list of species shared among the Xingu and Tapajós basins.(AU)

Uma nova espécie de Ancistrus é descrita das bacias dos rios Tapajós e Xingu. Distingue-se de suas congêneres pelo padrão de colorido único, que consiste em listras vermiculadas escuras em quase todo o corpo, e também pela combinação de características como cabeça estreita, maior distância entre as narinas e ausência de séries de grandes odontódeos nas placas laterais. Adicionalmente, a nova espécie pode ser diferenciada de suas congêneres da bacia do rio Tapajós pela presença de uma nadadeira adiposa totalmente desenvolvida (vs. nadadeira adiposa ausente em Ancistrus parecis e A. tombador, nadadeira adiposa vestigial ou ausente em A. krenakarore). Ela difere de A. ranunculus, a única outra espécie do rio Xingu, pelo padrão de colorido, menor tamanho corporal, menor abertura branquial e menor largura entre os cleitros. O novo táxon adiciona um novo registro à lista de espécies compartilhadas entre as bacias do Xingu e Tapajós.(AU)

A new species of the seasonal killifish genus Moema (Cyprinodontiformes: Rivulidae) from the Piraí watershed in the Southwest Amazon basin

Moema, a genus of the Rivulidae family, currently comprises 20 valid species. Most of these species inhabit temporary pools in the Amazon basin and only one species is found in the upper Paraguay basin. A new member of this genus from the upper Río Madeira drainage is here described. Males of the new species differ from all congeners by the combination of a color pattern consisting of oblique, sometimes chevron-like, irregular rows of red and light blue double-dots on body; dark yellowish to golden pectoral fins with no visible markings; and a stripe pattern on the ventral section of caudal fin with a very narrow black marginal line, intermittent or even absent. The existence of infrageneric species groups within Moema, the geographic distribution and taxonomic aspects of the species present in the Ríos Mamoré and Iténez/Guaporé rivers drainages, and the observation of intraspecific aggression between males and amphibious lifestyle in the new species are discussed.

Moema, un género de la familia Rivulidae, actualmente comprende 20 especies válidas. La mayoría de estas especies habitan en charcos temporales de la cuenca amazónica y solo una especie se encuentra en la cuenca alta del Río Paraguay. Un nuevo miembro de este género de la cuenca alta del Río Madeira es descrito aquí. Los machos de la especie nueva se diferencian de todos los congéneres por la combinación de un patrón de color de filas irregulares oblicuas, a veces en forma de chevrón, de puntos dobles rojos y azul claro en el cuerpo; aletas pectorales de color amarillento oscuro a dorado sin marcas visibles; y un patrón de rayas en la sección ventral de la aleta caudal con una línea marginal negra muy estrecha, intermitente o incluso ausente. Se discute la existencia de grupos infragenéricos de especies dentro de Moema, la distribución geográfica y aspectos taxonómicos de las especies presentes en el drenaje de los Ríos Mamoré e Iténez/Guaporé, y la observación de agresión intraespecífica entre machos y estilo de vida anfibio en la nueva especie.

Corticoterapia intralesional no tratamento de otohematoma em cães / Treating otohematomas in dogs with intra-lesional corticotherapy

Background: Otohematoma is common in dogs and is characterized by blood accumulation between the skin and cartilageof the outer ear. While the etiology is related to trauma, most cases have a predisposing condition. Treatment must drainthe hematoma and maintain appropriate skin apposition to the ear cartilage. Treatment can be surgical, but there are alsoconservative options such as puncture drainage, followed by intralesional injection of glucocorticoids. This alternativemethod is less invasive than surgery, with an equivalent success rate. This study aimed to describe intralesional injectionof corticosteroids for the treatment of dogs with otohematoma at a veterinary clinic.Materials, Methods & Results: Otohematoma was diagnosed and treated in 23 dogs (14 males and 9 females, weighing9.6 ± 2.7 kg) at a reference private veterinary clinic. The dogs were chemically restrained, and their ears were cleanedwith chlorhexidine. The lower face of each ear with otohematoma was then punctured with a needle coupled to a syringeto drain the liquid. Following drainage, the equipment was removed and the collected liquid volume was measured. A0.5 mg/kg dose of methylprednisolone acetate was prepared and diluted in saline (0.9% NaCl) to a volume equivalent to1/10 of the previously drained content volume from the otohematoma. The prepared solution was then injected into thedrained ear. Additionally, each patient was treated for the original cause of the otohematoma, according to conventionalprotocols. Eight animals (34.78%) had bilateral otohematoma and 15 (65.22%) presented with unilateral lesions. Leukocytosis was observed in most patients. Other laboratory alterations present in the studied dogs were thrombocytopenia,leukopenia, and anemia. Twenty (86.96%) patients presented with otitis externa. All patients were reassessed one weekafter the initial treatment, and 19 (82.60%) fully recovered. The remaining...

Corticoterapia intralesional no tratamento de otohematoma em cães / Treating otohematomas in dogs with intra-lesional corticotherapy

Background: Otohematoma is common in dogs and is characterized by blood accumulation between the skin and cartilageof the outer ear. While the etiology is related to trauma, most cases have a predisposing condition. Treatment must drainthe hematoma and maintain appropriate skin apposition to the ear cartilage. Treatment can be surgical, but there are alsoconservative options such as puncture drainage, followed by intralesional injection of glucocorticoids. This alternativemethod is less invasive than surgery, with an equivalent success rate. This study aimed to describe intralesional injectionof corticosteroids for the treatment of dogs with otohematoma at a veterinary clinic.Materials, Methods & Results: Otohematoma was diagnosed and treated in 23 dogs (14 males and 9 females, weighing9.6 ± 2.7 kg) at a reference private veterinary clinic. The dogs were chemically restrained, and their ears were cleanedwith chlorhexidine. The lower face of each ear with otohematoma was then punctured with a needle coupled to a syringeto drain the liquid. Following drainage, the equipment was removed and the collected liquid volume was measured. A0.5 mg/kg dose of methylprednisolone acetate was prepared and diluted in saline (0.9% NaCl) to a volume equivalent to1/10 of the previously drained content volume from the otohematoma. The prepared solution was then injected into thedrained ear. Additionally, each patient was treated for the original cause of the otohematoma, according to conventionalprotocols. Eight animals (34.78%) had bilateral otohematoma and 15 (65.22%) presented with unilateral lesions. Leukocytosis was observed in most patients. Other laboratory alterations present in the studied dogs were thrombocytopenia,leukopenia, and anemia. Twenty (86.96%) patients presented with otitis externa. All patients were reassessed one weekafter the initial treatment, and 19 (82.60%) fully recovered. The remaining...(AU)

An experimental pleural drainage device in hypertensive pneumothorax

ABSTRACT Purpose To develop a specific device for pleural drainage in hypertensive pneumothorax. Methods The prototype was modeled from the free version of a 3D modeling application, printed on a 3D printer using ABS® plastic material, and tested in a pleural drainage simulator. Results Pleural drainage in the simulator using the prototype was feasible and reproducible. Conclusions While the prototype is functional in the simulator, it requires improvement and refinement for use in humans.

Utilização de curativo a vácuo de baixo custo para cicatrização de fratura de casco com ruptura de cavidade celomática em um Chelonia carbonaria / Use of cost-effective vacuum-assisted closure technique for shell fracture Repair and coelomic cavity rupture healing in a Chelonia carbonaria

Background: The vacuum-assisted closure (VAC) therapy system has been used as a noninvasive wound managementtechnique for shell damage in Chelonians. These animals are excellent candidates for VAC therapy because of their uniqueshell anatomy consists of dermal bones, which make bandage placement easier. Beyond that, they are suited for this technique behaviorally, because they are not inclined to remove the vacuum system intentionally. Considering the possibilityof Testudines shell repair with the use of less invasive techniques, that result no additional dermal bone lesions, the objective of the present study is to describe the adaptation of a vacuum dressing protocol using low-cost and easily accessiblematerials for post-traumatic shell healing of a specimen of Chelonoidis carbonaria.Case: A specimen of tortoise (Chelonoidis carbonaria), a male, weighing 630 g, approximately 3-year-old, was received atthe Veterinary Hospital - Wild Animals Sector in the Federal University of Pará (UFPA) after of being run over by a vehicle.On physical examination, fractures of the dermal plaques and underlying bone structures were found, with rupture of thecoelomic cavity. In addition, there was exposure and incarceration of an intestinal loop, with the presence of bleeding.According to the findings of the physical screening examination, the patient’s prognosis was defined as good, as describedin the literature that specifically focused on chelonian shell injuries. The animal was sent to the diagnostic imaging sector.Then, drug therapy was provided for pain control, vitamins were administered for nutritional support, and antibiotics andanti-inflammatory drugs were instituted. For the closure of the shell and coelomic cavity, a bandage was used with theVAC therapy system adapted as described for Chelonians in previous reports. After a complete osteosynthesis and closureof the coelomic cavity, repair of the integumentary component of the shell was...

Utilização de curativo a vácuo de baixo custo para cicatrização de fratura de casco com ruptura de cavidade celomática em um Chelonia carbonaria / Use of cost-effective vacuum-assisted closure technique for shell fracture Repair and coelomic cavity rupture healing in a Chelonia carbonaria

Background: The vacuum-assisted closure (VAC) therapy system has been used as a noninvasive wound managementtechnique for shell damage in Chelonians. These animals are excellent candidates for VAC therapy because of their uniqueshell anatomy consists of dermal bones, which make bandage placement easier. Beyond that, they are suited for this technique behaviorally, because they are not inclined to remove the vacuum system intentionally. Considering the possibilityof Testudines shell repair with the use of less invasive techniques, that result no additional dermal bone lesions, the objective of the present study is to describe the adaptation of a vacuum dressing protocol using low-cost and easily accessiblematerials for post-traumatic shell healing of a specimen of Chelonoidis carbonaria.Case: A specimen of tortoise (Chelonoidis carbonaria), a male, weighing 630 g, approximately 3-year-old, was received atthe Veterinary Hospital - Wild Animals Sector in the Federal University of Pará (UFPA) after of being run over by a vehicle.On physical examination, fractures of the dermal plaques and underlying bone structures were found, with rupture of thecoelomic cavity. In addition, there was exposure and incarceration of an intestinal loop, with the presence of bleeding.According to the findings of the physical screening examination, the patients prognosis was defined as good, as describedin the literature that specifically focused on chelonian shell injuries. The animal was sent to the diagnostic imaging sector.Then, drug therapy was provided for pain control, vitamins were administered for nutritional support, and antibiotics andanti-inflammatory drugs were instituted. For the closure of the shell and coelomic cavity, a bandage was used with theVAC therapy system adapted as described for Chelonians in previous reports. After a complete osteosynthesis and closureof the coelomic cavity, repair of the integumentary component of the shell was...(AU)

Description of a new species of Tatia from rio Tocantins drainage, central Brazil, with notes on Tatia simplex Mees, 1974 (Siluriformes, Auchenipteridae)

Tatia comprises twenty-five valid species, distributed in the main inland watersheds of South America, including the Orinoco, Essequibo, and coastal rivers of Suriname, Amazon, upper rio Paraná and São Francisco basins. A new species is described from tributaries of upper rio Manuel Alves on uplands of Serra Geral do Tocantins plateau, Tocantins State, Brazil. It is promptly distinguished from all congeners, except Tatia britskii, due to absence of an adipose fin. It differs from T. britskii by the longer caudal peduncle length (24.1-30.5% SL, mean 25.3 vs. 20.0-22.7, mean 20.9); caudal peduncle depth (10.9-16.8 SL, mean 14.1 vs. 9.4-10.5, mean 9.8), and anterior cranial border with mesethmoid width equals its length (vs. width approximately three times its length in T. britskii). Additionally, information regarding the poorly known species Tatia simplex originally described from rio das Mortes, is provided.(AU)

Tatia abriga vinte e cinco espécies válidas distribuídas nos maiores sistemas fluviais de águas interiores da América do Sul, como o Orinoco, Essequibo, e rios costeiros do Suriname, Amazonas, alto Paraná e São Francisco. Uma espécie nova é descrita para tributários do alto rio Manuel Alves, nos contrafortes da Serra Geral do Tocantins, estado do Tocantins, Brasil. A espécie nova é facilmente distinguida de todos os congêneres, exceto Tatia britskii, pela ausência de nadadeira adiposa. Difere de T. britskii pelo maior comprimento do pedúnculo caudal (24,1-30,5% CP, média 25,3 vs. 20,0-22,7% CP, média 20,9); altura do pedúnculo caudal (10,9-16,8 SL, mean 14,1 vs. 9,4-10,5, mean 9,8) e margem craniana anterior com largura do mesetmoide igual a seu comprimento (vs. largura aproximadamente três vezes no seu comprimento em T. britskii). Adicionalmente, são fornecidas informações sobre Tatia simplex, uma espécie pouco conhecida, descrita para o rio das Mortes.(AU)

Description of a new species of Tatia from rio Tocantins drainage, central Brazil, with notes on Tatia simplex Mees, 1974 (Siluriformes, Auchenipteridae)

Tatia comprises twenty-five valid species, distributed in the main inland watersheds of South America, including the Orinoco, Essequibo, and coastal rivers of Suriname, Amazon, upper rio Paraná and São Francisco basins. A new species is described from tributaries of upper rio Manuel Alves on uplands of Serra Geral do Tocantins plateau, Tocantins State, Brazil. It is promptly distinguished from all congeners, except Tatia britskii, due to absence of an adipose fin. It differs from T. britskii by the longer caudal peduncle length (24.1-30.5% SL, mean 25.3 vs. 20.0-22.7, mean 20.9); caudal peduncle depth (10.9-16.8 SL, mean 14.1 vs. 9.4-10.5, mean 9.8), and anterior cranial border with mesethmoid width equals its length (vs. width approximately three times its length in T. britskii). Additionally, information regarding the poorly known species Tatia simplex originally described from rio das Mortes, is provided.(AU)

Tatia abriga vinte e cinco espécies válidas distribuídas nos maiores sistemas fluviais de águas interiores da América do Sul, como o Orinoco, Essequibo, e rios costeiros do Suriname, Amazonas, alto Paraná e São Francisco. Uma espécie nova é descrita para tributários do alto rio Manuel Alves, nos contrafortes da Serra Geral do Tocantins, estado do Tocantins, Brasil. A espécie nova é facilmente distinguida de todos os congêneres, exceto Tatia britskii, pela ausência de nadadeira adiposa. Difere de T. britskii pelo maior comprimento do pedúnculo caudal (24,1-30,5% CP, média 25,3 vs. 20,0-22,7% CP, média 20,9); altura do pedúnculo caudal (10,9-16,8 SL, mean 14,1 vs. 9,4-10,5, mean 9,8) e margem craniana anterior com largura do mesetmoide igual a seu comprimento (vs. largura aproximadamente três vezes no seu comprimento em T. britskii). Adicionalmente, são fornecidas informações sobre Tatia simplex, uma espécie pouco conhecida, descrita para o rio das Mortes.(AU)

Man-made soil drainage alters the vegetation structure and woody species distribution in campo de murundus

Campos de murundus work as recharge zones and are important for the maintenance of water resources in Brazilian Cerrado. However, with the expansion of the agricultural frontier, this ecosystem may disappear or suffer high anthropogenic disturbances. The aim of the study was to evaluate the structure and distribution of woody species, after the implantation of artificial drainage channels in campo de murundus, in plots near and distant of the drains in the soil. We sampled woody individuals with ≥ 3 cm diameter at the base, and established 20 permanent plots of 20×50 m, 10 of which were between 0-20 m of the drains (edge) and 10 between 150-200 m distant from the drains (interior), totaling two hectares of survey. We recorded 47 species with total density of 230.5 ind.ha-1 and total basal area of 1.331 m2 ha-1. The diversity index of species was H’ = 3.18. We recorded higher density and basal area in the edge, and differential distribution and occupation of woody species in the microrelief of the murundus. This provides us strong evidence that the drains have altered the vegetation structure, especially in the edge of the remnant campos de murundus turning the vegetation woodier and denser.

Man-made soil drainage alters the vegetation structure and woody species distribution in campo de murundus

Campos de murundus work as recharge zones and are important for the maintenance of water resources in Brazilian Cerrado. However, with the expansion of the agricultural frontier, this ecosystem may disappear or suffer high anthropogenic disturbances. The aim of the study was to evaluate the structure and distribution of woody species, after the implantation of artificial drainage channels in campo de murundus, in plots near and distant of the drains in the soil. We sampled woody individuals with ≥ 3 cm diameter at the base, and established 20 permanent plots of 20×50 m, 10 of which were between 0-20 m of the drains (edge) and 10 between 150-200 m distant from the drains (interior), totaling two hectares of survey. We recorded 47 species with total density of 230.5 ind.ha-1 and total basal area of 1.331 m2 ha-1. The diversity index of species was H = 3.18. We recorded higher density and basal area in the edge, and differential distribution and occupation of woody species in the microrelief of the murundus. This provides us strong evidence that the drains have altered the vegetation structure, especially in the edge of the remnant campos de murundus turning the vegetation woodier and denser.(AU)

Equine heterotopic polyodontia: a case report / Poliodontia heterotópica equina: relato de caso / Poliodontia heterotópica equina: relato de caso

Heterotopic polyodontia have been described in most domestic species. Known as a dentigerous cyst but appropriately called heterotopic polyiodontics, it rarely occurs in horses, however it is easily recognized as a congenital defect. The cysts usually associated with this condition contain part or all of dental structures. The heterotopic tooth or dental structure is usually adhered to the temporal bone and surrounded by a secretory membrane, with a accumulation of exudate and draining tract along the proximal pinna or directly over the cyst. This case report describes in a 2.5-year-old female quarter horse, weighing 430 kg, with a heterotopic polyodontia that contained two easily small's identifiable dental structures, presented for evaluation of a chronic intermittent mucopurulent exudate discharge from the right sub-auricular region in the mastoid process of the temporal bone and a drainage tract near the right pinna. The diagnosis is established with clinical, radiographic, ultrasound and confirmed by histopathological examination of the structures removed. The cystic capsule was surgically removed, measuring approximately 3.0 x 2.3 cm in diameter, filled with mucopurulent exudate and containing two dental structures within 0.5 cm in diameter and 0.2 cm in diameter. Microscopic examination revealed a cystic area covered by stratified squamous epithelium with a lympho-histio-plasmatic cell infiltrate in the dermis, which was compatible with heterotopic polyodontia. Postoperatively, there were no neuromotor sequelae and wound healing evolved positively.(AU)

A poliodontia heterotópica tem sido descrita na maioria das espécies domésticas. Conhecido como cisto dentígero, mas apropriadamente denominado poliodontia heterotópica, raramente ocorre em equinos, porém é facilmente reconhecido como defeito congênito. Os cistos geralmente associados a essa condição contêm parte ou toda as estruturas dentarias. O dente heterotópico ou estrutura dentaria é comumente encontrado aderido ao osso temporal e envolvido por uma membrana secretora, com acúmulo de exsudato e trajeto de drenagem ao longo do pavilhão auricular proximal ou diretamente sobre o cisto. Este relato descreve um caso de em um equino,fêmea, quarto de milha, com 2,5 anos de idade, pesando 430 kg, com poliodontia heterotópica que continha duas estruturas dentarias facilmente indentificadas, apresentando um orifício na região sub-auricular direita no processo mastóideo do osso temporal, com secreção mucopurulenta intermitente e crônica, próximo ao pavilhão auricular direito. O diagnóstico foi estabelecido com exame clínico, radiográfico, ultrassonográfico e confirmado pelo exame histopatológico das estruturas retiradas. A cápsula cística foi removida cirurgicamente medindo aproximadamente 3,0 x 2,3 cm de diâmetro, preenchida com exsudato mucopurulento e contendo em seu interior duas estruturas dentárias medindo cerca de 0,5 cm de diâmetro e 0,2 cm de diâmetro. O exame microscópico revelou área cística revestida por epitélio escamoso estratificado com infiltrado linfo-histo-plasmocitário na derme, compatível com poliodontia heterotópica. No pós-operatório não houve sequelas neuromotoras e a cicatrização da ferida evoluiu positivamente.(AU)

La poliodontia heterotópica ha sido descrita en la mayoría de las especies domésticas. Conocido como quiste dentígero, pero apropiadamente llamado poliodontia heterotópica, raramente ocurre en equinos, sin embargo se reconoce fácilmente como un defecto congénito. Los quistes generalmente asociados a esa condición contienen parte o la totalidad de las estructuras dentales. El diente heterotópico o estructura dental se encuentra comúnmente adherido al hueso temporal y rodeado por una membrana secretora, con acumulación de exudado y vía de drenaje a lo largo del pabellón auricular proximal o directamente sobre el quiste. En este informe se describe el caso de una hembra, cuarto de milla, de 2,5 años de edad, con 430 kg de peso, presentando poliodontia heterotópica que contenía dos estructuras dentales fácilmente identificables, con un orificio en la región subauricular derecha en el proceso mastoides del hueso temporal, secreción mucopurulenta intermitente y crónica próximo al oído derecho. El diagnóstico se estableció con examen clínico, radiográfico, ecográfico y se confirmó con el examen histopatológico de las estructuras extirpadas. La cápsula quística se extrajo quirúrgicamente midiendo aproximadamente 3,0 x 2,3 cm de diámetro, rellena con exudado mucopurulento y conteniendo en su interior dos estructuras dentales que medían aproximadamente 0,5 cm de diámetro y 0,2 cm de diámetro. El examen microscópico reveló una zona quística cubierta por un epitelio escamoso estratificado con infiltrado linfo-histo-plasmocítico en la dermis, compatible con poliodontia heterotópica. En el postoperatorio no se produjeron secuelas neuromotoras y la cicatrización de la herida evolucionó positivamente.(AU)

Equine heterotopic polyodontia: a case report / Poliodontia heterotópica equina: relato de caso / Poliodontia heterotópica equina: relato de caso

Heterotopic polyodontia have been described in most domestic species. Known as a dentigerous cyst but appropriately called heterotopic polyiodontics, it rarely occurs in horses, however it is easily recognized as a congenital defect. The cysts usually associated with this condition contain part or all of dental structures. The heterotopic tooth or dental structure is usually adhered to the temporal bone and surrounded by a secretory membrane, with a accumulation of exudate and draining tract along the proximal pinna or directly over the cyst. This case report describes in a 2.5-year-old female quarter horse, weighing 430 kg, with a heterotopic polyodontia that contained two easily small's identifiable dental structures, presented for evaluation of a chronic intermittent mucopurulent exudate discharge from the right sub-auricular region in the mastoid process of the temporal bone and a drainage tract near the right pinna. The diagnosis is established with clinical, radiographic, ultrasound and confirmed by histopathological examination of the structures removed. The cystic capsule was surgically removed, measuring approximately 3.0 x 2.3 cm in diameter, filled with mucopurulent exudate and containing two dental structures within 0.5 cm in diameter and 0.2 cm in diameter. Microscopic examination revealed a cystic area covered by stratified squamous epithelium with a lympho-histio-plasmatic cell infiltrate in the dermis, which was compatible with heterotopic polyodontia. Postoperatively, there were no neuromotor sequelae and wound healing evolved positively.(AU)

A poliodontia heterotópica tem sido descrita na maioria das espécies domésticas. Conhecido como cisto dentígero, mas apropriadamente denominado poliodontia heterotópica, raramente ocorre em equinos, porém é facilmente reconhecido como defeito congênito. Os cistos geralmente associados a essa condição contêm parte ou toda as estruturas dentarias. O dente heterotópico ou estrutura dentaria é comumente encontrado aderido ao osso temporal e envolvido por uma membrana secretora, com acúmulo de exsudato e trajeto de drenagem ao longo do pavilhão auricular proximal ou diretamente sobre o cisto. Este relato descreve um caso de em um equino,fêmea, quarto de milha, com 2,5 anos de idade, pesando 430 kg, com poliodontia heterotópica que continha duas estruturas dentarias facilmente indentificadas, apresentando um orifício na região sub-auricular direita no processo mastóideo do osso temporal, com secreção mucopurulenta intermitente e crônica, próximo ao pavilhão auricular direito. O diagnóstico foi estabelecido com exame clínico, radiográfico, ultrassonográfico e confirmado pelo exame histopatológico das estruturas retiradas. A cápsula cística foi removida cirurgicamente medindo aproximadamente 3,0 x 2,3 cm de diâmetro, preenchida com exsudato mucopurulento e contendo em seu interior duas estruturas dentárias medindo cerca de 0,5 cm de diâmetro e 0,2 cm de diâmetro. O exame microscópico revelou área cística revestida por epitélio escamoso estratificado com infiltrado linfo-histo-plasmocitário na derme, compatível com poliodontia heterotópica. No pós-operatório não houve sequelas neuromotoras e a cicatrização da ferida evoluiu positivamente.(AU)

La poliodontia heterotópica ha sido descrita en la mayoría de las especies domésticas. Conocido como quiste dentígero, pero apropiadamente llamado poliodontia heterotópica, raramente ocurre en equinos, sin embargo se reconoce fácilmente como un defecto congénito. Los quistes generalmente asociados a esa condición contienen parte o la totalidad de las estructuras dentales. El diente heterotópico o estructura dental se encuentra comúnmente adherido al hueso temporal y rodeado por una membrana secretora, con acumulación de exudado y vía de drenaje a lo largo del pabellón auricular proximal o directamente sobre el quiste. En este informe se describe el caso de una hembra, cuarto de milla, de 2,5 años de edad, con 430 kg de peso, presentando poliodontia heterotópica que contenía dos estructuras dentales fácilmente identificables, con un orificio en la región subauricular derecha en el proceso mastoides del hueso temporal, secreción mucopurulenta intermitente y crónica próximo al oído derecho. El diagnóstico se estableció con examen clínico, radiográfico, ecográfico y se confirmó con el examen histopatológico de las estructuras extirpadas. La cápsula quística se extrajo quirúrgicamente midiendo aproximadamente 3,0 x 2,3 cm de diámetro, rellena con exudado mucopurulento y conteniendo en su interior dos estructuras dentales que medían aproximadamente 0,5 cm de diámetro y 0,2 cm de diámetro. El examen microscópico reveló una zona quística cubierta por un epitelio escamoso estratificado con infiltrado linfo-histo-plasmocítico en la dermis, compatible con poliodontia heterotópica. En el postoperatorio no se produjeron secuelas neuromotoras y la cicatrización de la herida evolucionó positivamente.(AU)

Mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and inflammation

Purpose:To investigate the effect of mesenteric lymph drainage on the spleen injury and the expressions of inflammatory cytokines in splenic tissue in mice following hemorrhagic shock.Methods:Male C57 mice were randomly divided into the sham shock, shock and shock+drainage groups. The mice in both shock and shock+drainage groups suffered femoral artery bleeding, maintained mean arterial pressure (MAP) of 40±2 mmHg for 90 min, and were resuscitated. And mesenteric lymph drainage was performed in the shock+drainage group at the time of resuscitation. After three hours of resuscitation, the splenic tissues were harvested for the histological observation and protein and mRNA expression analysis of cytokines.Results:The spleen in the shock group revealed a significantly structural damage and increased mRNA expressions of MyD88 and TRAF6 and protein expressions of TIPE2, MyD88, TRIF and TRAF3 compared to the sham group. By contrast, the splenic pathological injury in the shock+drainage group was alleviated significantly, and the mRNA and protein expressions of TIPE2, MyD88, TRIF, TRAF3 and TRAF6 were significantly lower than those in the shock group.Conclusion:These results indicate that post-hemorrhagic shock mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and the expressions of inflammatory cytokines.(AU)

River waters near to agricultural sites in the Northeastern Brazil (Maranhão State) cause genetic damage in Nile Tilapia (Oreochromis niloticus)

Munim River is a small basin (15,918.04 km2, 320,000 inhabitants) in northeastern Brazil (Maranhão state), whose water quality has been threatened by pesticides, untreated domestic and industrial wastewater discharges, and solid waste disposal ( Presoti and Cutrim, 2011 ). These pollutants may bring several problems to the aquatic biota, such as mutagenic and carcinogenic effects ( Bolognesi and Hayashi, 2011 ) including risks to human health. Therefore, it is critical to understand the potential impacts of xenobiotics on this river basin, especially their genotoxic and mutagenic effects. We investigated the genotoxic and mutagenic potential of the Munim River waters to assess the impact of the presence of xenobiotics on the aquatic biota.(AU)

Molecular phylogeny and biogeographic history of the Neotropical tribe Glandulocaudini (Characiformes: Characidae: Stevardiinae)

Although former studies on systematics and biogeography represent a progress on the knowledge of the tribe Glandulocaudini, none was grounded on molecular evidence. Thus, the first hypothesis of relationships for the tribe based on a multilocus analysis is presented, including all genera and most of the valid species. DNA sequences of Glandulocauda caerulea and Mimagoniates sylvicola were analyzed for the first time. A molecular clock analysis was used to estimate the origin of the Glandulocaudini and the approximate timing of cladogenetic events within the group. Glandulocaudini was recovered as monophyletic. No hypothesis recovered Glandulocauda as monophyletic, since G. melanopleura is sister to Lophiobrycon weitzmani while G. caerulea is closely related to Mimagoniates. The relationships within the latter genus were resolved. The molecular clock results indicate the origin of the Glandulocaudini during the Miocene with diversification in the group occurring from Neogene to Pleistocene. These results corroborated the hypothesis that its origin took place on the Brazilian crystalline shield with the subsequent occupation of the Atlantic Coastal drainages. Apparently, Pleistocene sea-level fluctuations might have shaped the distribution pattern of some species in Glandulocaudini.(AU)

Embora estudos prévios sobre sistemática e biogeografia representam um avanço no conhecimento da tribo Glandulocaudini, nenhum foi baseado em evidência molecular. Assim, a primeira hipótese de relações para a tribo com base em uma análise multilocus é apresentada, incluindo todos os gêneros e a maioria das espécies válidas. Sequências de DNA de Glandulocauda caerulea e Mimagoniates sylvicola foram analisadas pela primeira vez. Uma análise de relógio molecular foi utilizada para estimar a origem de Glandulocaudini e datas aproximadas de eventos cladogenéticos dentro do grupo. Glandulocaudini foi recuperada como monofilética. Nenhuma hipótese recuperou Glandulocauda como monofilético, uma vez que G. melanopleura é irmã de Lophiobrycon weitzmani e G. caerulea está proximamente relacionada a Mimagoniates. As relações dentro deste último gênero foram resolvidas. Os resultados do relógio molecular indicam que Glandulocaudini originou-se durante o Mioceno, com diversificação dentro do grupo ocorrendo desde o Neogeno até o Pleistoceno. Estes resultados corroboram a hipótese da sua origem no escudo cristalino brasileiro, com a subsequente ocupação das drenagens costeiras atlânticas. Aparentemente, as flutuações pleistocênicas do nível do mar podem ter moldado o padrão de distribuição de algumas espécies em Glandulocaudini.(AU)