Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 192
Filtrar
1.
Arq. bras. med. vet. zootec. (Online) ; 75(3): 404-414, 2023. ilus, graf
Artigo em Inglês | VETINDEX | ID: biblio-1436913

Resumo

The aim of the study was to investigate the nephrotoxicity of doxorubicin in cats with malignant neoplasms of the mammary gland. All selected cats did not present comorbidities such as nephropathies and/or cardiomyopathies, confirmed by physical and laboratory tests, underwent radical mastectomy associated with regional lymph node excision and were treated with a protocol based on doxorubicin. Renal markers of urea, creatinine, symmetrical dimethylarginine were evaluated during all the treatment, and two months after the end. Abdominal ultrasound, urinalysis and biochemical analysis of protein, urinary creatinine and urinary GGT were performed in the beginning and two months after the end of the treatment, to evaluate possible renal alterations. Six cats did not present renal alterations in any exams performed during the study. Two cats presented azotemia during this study, and one of them stopped the treatment early, because of the intense azotemia. Clinical imaging and laboratory monitoring of patients throughout the treatment is essential, including the measurement of analytes that detect kidney changes early. Thus, it is emphasized that doxorubicin is a safe drug for use in non-nephropathic cats.


O objetivo do estudo foi investigar a nefrotoxicidade da doxorrubicina em gatas com neoplasias malignas da glândula mamária. Todas as gatas selecionadas não apresentaram comorbidades como nefropatias e/ou cardiomiopatias, comprovadas por exames físicos e laboratoriais, foram submetidas à mastectomia radical associada à exérese de linfonodos regionais e tratadas com protocolo baseado em doxorrubicina. Os marcadores renais ureia, creatinina, dimetilarginina simétrica foram avaliados durante todo o tratamento e dois meses após o término. Ultrassonografia abdominal, urinálise e análise bioquímica de proteínas, creatinina urinária e GGT urinária foram realizadas no início e dois meses após o término do tratamento, para avaliar possíveis alterações renais. Seis gatas não apresentaram alterações renais em nenhum dos exames realizados durante o estudo. Duas gatas apresentaram azotemia durante este estudo, e uma delas interrompeu o tratamento precocemente, devido à intensa azotemia. A individualidade de cada paciente deve ser sempre considerada, pois são muitas as variáveis. O monitoramento clínico, com exames complementares, tais como bioquímicos séricos e de imagem dos pacientes durante todo o tratamento, é essencial, especialmente a mensuração de analitos que detectam alterações renais precocemente. Diante disso, ressalta-se que a doxorrubicina é um medicamento seguro para utilização em gatas não nefropatas.


Assuntos
Animais , Gatos , Doxorrubicina/toxicidade , Doenças do Gato , Neoplasias Mamárias Animais
2.
Anim. Reprod. (Online) ; 19(3): e20210069, set. 2022. tab
Artigo em Inglês | VETINDEX | ID: biblio-1393202

Resumo

Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.(AU)


Assuntos
Animais , Masculino , Cães , Processamento de Imagem Assistida por Computador , Criopreservação/veterinária , Análise do Sêmen/veterinária , Sobrevivência Celular
3.
Braz. j. biol ; 822022.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1468758

Resumo

Abstract Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.


Resumo A síntese verde de nanopartículas de prata (AgNPs) é uma abordagem ecologicamente correta, econômica e promissora para a descoberta de novas terapêuticas. O objetivo do presente trabalho foi sintetizar biogênica, caracterizar AgNPs usando extratos de sementes de três variedades economicamente importantes de tamareira (Iklas, Irziz e Shishi) e avaliar suas atividades bacterianas antipatogênicas. AgNPs foram sintetizados e caracterizados usando microscopia eletrônica e análise de infravermelho por transformada de Fourier. As atividades bactericidas de AgNPs contra cinco diferentes patógenos bacterianos, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus resistente à meticilina e Streptococcus pneumoniae, foram determinadas in vitro. Em particular, foram investigadas alterações na integridade da membrana de cepas bacterianas virulentas em resposta a AgNPs. Os resultados da lactato desidrogenase, dos ensaios da atividade da fosfatase alcalina e da medição do potencial de membrana revelaram que os efeitos citotóxicos dos AgNPs estavam principalmente centrados na membrana plasmática das células bacterianas, levando à perda de sua integridade e, eventualmente, à morte celular. A síntese verde de AgNPs é uma estratégia eficiente, econômica e promissora para combater cepas virulentas resistentes a antibióticos.

4.
Acta sci. vet. (Impr.) ; 50: Pub.1856-2022. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1458531

Resumo

Background: Equine cutaneous habronemiasis is common in the distal regions of the limbs. Organophosphates, appliedsystemically, one previously used treatment, which is highly effective, but currently in disuse, due to the risks of intoxication. Regional perfusion is a potential technique for distal limb wounds, since, in addition to being used in low doses, itprevents systemic circulation of the drug and possible intoxication, and has a lower treatment cost. The current work aimedto perform clinical, laboratory, and venography evaluations of the use of trichlorfon in regional intravenous perfusion, asa possible form of treatment for cutaneous habronemosis in the distal region of equine limbs.Materials, Methods & Results: Twelve equines were used, divided into 2 groups, with the left thoracic limb (LTL) beingthe test limb, and the right thoracic limb (RTL) the control limb. At moment zero (M0), distal radiography and venographywere performed. The tourniquet was then loosened and after 5 min, at moment one (M1), the tourniquet was repositionedfor 1.25 mg/kg (G1) and 5.5 mg/kg (G2) trichlorfon injections into the left thoracic limb, diluted in 20 mL of Ringer’s lactate solution, and 20 mL of Ringer’s lactate solution was applied to the right thoracic limb. The tourniquet was maintainedfor 30 min after infusion in both groups. At moment 2 (M2), 4 days later, blood tests, radiography, and venography wererepeated. Every day between M0 and M2, physical examinations were performed, including measurement of the pastern,fetlock, and coronet band, and a lameness examination. There were no significant alterations in clinical parameters, behavior, and appetite. In the blood cell count, there was an increase in leukocytes on D4 in G2, although remaining withinthe reference values for the species. The biochemical tests showed no alterations. There were no changes in the circumferences evaluated and 4 equines from G2 presented lameness in the LTL and...


Assuntos
Animais , Cavalos , Infecções por Spirurida/terapia , Infecções por Spirurida/veterinária , Perfusão/veterinária , Triclorfon/administração & dosagem , Triclorfon/uso terapêutico , Flebografia/veterinária
5.
Acta sci. vet. (Impr.) ; 50(supl.1): Pub. 807, 2022. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1401362

Resumo

Background: Primary erythrocytosis is a rare myeloproliferative disorder in dogs and cats characterized by an autonomous proliferation of erythroid precursors in the bone marrow, with low to normal serum erythropoietin concentration, resulting in elevated red blood cell count, hematocrit and hemoglobin concentration. Clinical signs are associated with increased blood volume and viscosity, and may include erythema, hyperemic mucous membranes and neurological signs such as seizures and ataxia. In veterinary medicine, the diagnosis should be made by exclusion of secondary or relative causes, after complementary exams. This report aims to describe a case of primary erythrocytosis in a bitch. Case: A 4-year-old mixed-breed bitch was referred to the Veterinary Medical Teaching Hospital from UFRGS with 3 convulsive episodes related by the owner. A previous abdominal ultrasonography revealed splenomegaly and the electrocardiogram showed no abnormalities. No alterations were observed at the physical examination. The laboratorial blood tests demonstrated a persistent erythrocytosis, with high hematocrit, hemoglobin and red blood cells count, thrombocytopenia and neutropenia, and total plasmatic protein within the reference interval. The bone marrow cytology revealed reduced cellularity, normal myeloid:erythroid ratio, erythroid hyperplasia, mild myeloid hyperplasia and moderate myelofibrosis. The serum erythropoietin measurement was within the reference range, and the blood gas analysis detected a slight decrease in partial oxygen pressure. Therefore, no evidence of secondary conditions was observed and the diagnosis of primary erythrocytosis could be made. Discussion: Since there is no definitive method, the diagnosis of primary erythrocytosis could be based on the exclusion of all secondary and relative causes of erythrocytosis. The absence of clinical signs of dehydration and high serum albumin levels were findings that conduced for the exclusion of the relative form of the disturbance. The echocardiography and the abdominal ultrasonography ruled out any cardiopulmonary condition or kidney neoplasm, the most common causes of absolute secondary erythrocytosis. The persistently high hematocrit levels and red blood cell counts are significant for the suspicion of primary erythrocytosis, although thrombocytopenia and neutropenia are not commonly reported. The clinical signs of seizure were correlated with increased blood viscosity and reduced blood flow at the central nervous system. The blood gas analysis discarded the occurrence of systemic hypoxia, and the normal levels of erythropoietin gives higher evidence of the occurrence of an autonomous proliferation of the erythroid precursors within the bone marrow. The bone marrow cytology confirmed erythroid hyperplasia and the reduced cellularity that could be attributed to myelofibrosis. Myelofibrosis was described in humans with polycythemia vera, but there are no reports in veterinary, and this occurrence must be elucidated. An identical mutation in the JAK2 gene was observed in humans with polycythemia vera and dogs with primary erythrocytosis, and occurs in more than 50% of humans with myelofibrosis. Further investigations are necessary for veterinary medicine. In conclusion, the systematic approach of all organic systems and the assessment of complementary exams are necessary for the diagnostic of primary erythrocytosis in dogs. This condition should be considered in the differential diagnosis of any erythrocytosis, considering the guarded prognosis of this hematologic disorder.


Assuntos
Animais , Feminino , Cães , Policitemia/veterinária , Eritropoetina/análise , Mielofibrose Primária/veterinária , Esplenomegalia/veterinária , Ultrassonografia/veterinária
6.
Braz. j. biol ; 82: e242301, 2022. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1285599

Resumo

Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.


A síntese verde de nanopartículas de prata (AgNPs) é uma abordagem ecologicamente correta, econômica e promissora para a descoberta de novas terapêuticas. O objetivo do presente trabalho foi sintetizar biogênica, caracterizar AgNPs usando extratos de sementes de três variedades economicamente importantes de tamareira (Iklas, Irziz e Shishi) e avaliar suas atividades bacterianas antipatogênicas. AgNPs foram sintetizados e caracterizados usando microscopia eletrônica e análise de infravermelho por transformada de Fourier. As atividades bactericidas de AgNPs contra cinco diferentes patógenos bacterianos, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus resistente à meticilina e Streptococcus pneumoniae, foram determinadas in vitro. Em particular, foram investigadas alterações na integridade da membrana de cepas bacterianas virulentas em resposta a AgNPs. Os resultados da lactato desidrogenase, dos ensaios da atividade da fosfatase alcalina e da medição do potencial de membrana revelaram que os efeitos citotóxicos dos AgNPs estavam principalmente centrados na membrana plasmática das células bacterianas, levando à perda de sua integridade e, eventualmente, à morte celular. A síntese verde de AgNPs é uma estratégia eficiente, econômica e promissora para combater cepas virulentas resistentes a antibióticos.


Assuntos
Nanopartículas Metálicas , Staphylococcus aureus Resistente à Meticilina , Phoeniceae , Prata , Extratos Vegetais/farmacologia , Testes de Sensibilidade Microbiana , Antibacterianos/farmacologia
7.
Braz. j. biol ; 82: 1-8, 2022. ilus, graf, tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1468571

Resumo

Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.


A síntese verde de nanopartículas de prata (AgNPs) é uma abordagem ecologicamente correta, econômica e promissora para a descoberta de novas terapêuticas. O objetivo do presente trabalho foi sintetizar biogênica, caracterizar AgNPs usando extratos de sementes de três variedades economicamente importantes de tamareira (Iklas, Irziz e Shishi) e avaliar suas atividades bacterianas antipatogênicas. AgNPs foram sintetizados e caracterizados usando microscopia eletrônica e análise de infravermelho por transformada de Fourier. As atividades bactericidas de AgNPs contra cinco diferentes patógenos bacterianos, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus resistente à meticilina e Streptococcus pneumoniae, foram determinadas in vitro. Em particular, foram investigadas alterações na integridade da membrana de cepas bacterianas virulentas em resposta a AgNPs. Os resultados da lactato desidrogenase, dos ensaios da atividade da fosfatase alcalina e da medição do potencial de membrana revelaram que os efeitos citotóxicos dos AgNPs estavam principalmente centrados na membrana plasmática das células bacterianas, levando à perda de sua integridade e, eventualmente, à morte celular. A síntese verde de AgNPs é uma estratégia eficiente, econômica e promissora para combater cepas virulentas resistentes a antibióticos.


Assuntos
Antibiose , Bacillus subtilis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Nanopartículas/análise , Phoeniceae , Prata/análise , Staphylococcus aureus/efeitos dos fármacos , Streptococcus pneumoniae/efeitos dos fármacos , Microscopia , Técnicas In Vitro
8.
Braz. J. Biol. ; 82: 1-8, 2022. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: vti-31886

Resumo

Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.(AU)


A síntese verde de nanopartículas de prata (AgNPs) é uma abordagem ecologicamente correta, econômica e promissora para a descoberta de novas terapêuticas. O objetivo do presente trabalho foi sintetizar biogênica, caracterizar AgNPs usando extratos de sementes de três variedades economicamente importantes de tamareira (Iklas, Irziz e Shishi) e avaliar suas atividades bacterianas antipatogênicas. AgNPs foram sintetizados e caracterizados usando microscopia eletrônica e análise de infravermelho por transformada de Fourier. As atividades bactericidas de AgNPs contra cinco diferentes patógenos bacterianos, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus resistente à meticilina e Streptococcus pneumoniae, foram determinadas in vitro. Em particular, foram investigadas alterações na integridade da membrana de cepas bacterianas virulentas em resposta a AgNPs. Os resultados da lactato desidrogenase, dos ensaios da atividade da fosfatase alcalina e da medição do potencial de membrana revelaram que os efeitos citotóxicos dos AgNPs estavam principalmente centrados na membrana plasmática das células bacterianas, levando à perda de sua integridade e, eventualmente, à morte celular. A síntese verde de AgNPs é uma estratégia eficiente, econômica e promissora para combater cepas virulentas resistentes a antibióticos.(AU)


Assuntos
Phoeniceae , Nanopartículas/análise , Prata/análise , Antibiose , Bacillus subtilis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Streptococcus pneumoniae/efeitos dos fármacos , Microscopia , Técnicas In Vitro
9.
Vet. zootec ; 28: 1-5, 13 jan. 2021. tab
Artigo em Português | VETINDEX | ID: biblio-1503670

Resumo

Boa constrictor es una especie que se encuentra a menudo en criaderos y zoológicos, además de ser utilizada como animal de compañía. Estos animales son nativos del nuevo mundo y se encuentran desde México hasta el norte de Argentina. El objetivo del presente trabajo es generar valores morfométricos de las células que componen el tejido sanguíneo de individuos de la especie Boa constrictor, identificando sus tamaños promedios, que son importantes para determinar el estado de estas células, siendo principalmente útiles para clasificar las anemias. Los frotis sanguíneos se realizaron con muestras recolectadas de cuatro ejemplares atendidos en el Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), siendo dos machos y dos hembras. Las láminas se tiñeron utilizando la técnica de tinción panótica rápida y se observaron bajo un microscopio óptico (100X, inmersión). Para la morfometría, la captura de imágenes se realizó con la ayuda de una cámara digital Canon acoplada al microscopio, y la medición de las células se realizó con el software Zeiss® AxioVision Sample Images. Se midieron un total de 2007 células de sangre, siendo 590 eritrocitos, 365 heterofilos, 140 linfocitos, 454 azurofilos, 17 monocitos y 441 trombocitos. Se encontró que los eritrocitos y trombocitos mostraron mayores diferencias entre sus diámetros mayores y menores, dando a estas células una forma elíptica...


Boa constrictor is a species often found in breeding grounds and zoos, in addition to being used as a pet animal. These animals are native to the new world, being found from Mexico to northern Argentina. The aim of the present work is to generate morphometric values of the cells that make up the blood tissue of individuals of the Boa constrictor species, identifying their average sizes, which are important in determining the state of these cells, being mainly useful for classifying anemias. Blood strains were performed with samples collected from four specimens attended at the Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), being two males and two females. The slides were stained using the fast panotic staining technique and observed under an optical microscope (100X, immersion). For morphometry, image capture was performed with the aid of a Canon digital camera attached to the microscope, and the measurement of the cells was done with the Zeiss® AxioVision Sample Images software. A total of 2007 blood cells were measured, being 590 erythrocytes, 365 heterophiles, 140 lymphocytes, 454 azurophils, 17 monocytes and 441 thrombocytes. It was found that erythrocytes and thrombocytes showed greater differences between their larger and smaller diameters, giving these cells an elliptical shape. The other cells evaluated showed similar proportions between the diameters...


Boa constrictor é uma espécie frequentemente encontrada em criadouros e zoológicos, além de ser utilizada como animal de companhia. Esses animais são nativos do novo mundo, sendo encontrados do México ao norte da Argentina. O trabalho realizado possui como objetivo gerar valores morfométricos das células que compõem o tecido sanguíneo de indivíduos da espécie Boa constrictor, identificando seus tamanhos médios, que são importantes na determinação do estado destas células, sendo úteis principalmente para classificar anemias. Foram realizadas distensões sanguíneas com as amostras coletadas de quatro exemplares atendidos no Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), sendo dois machos e duas fêmeas. As lâminas foram coradas utilizando a técnica de coloração panóptico rápido e observadas ao microscópio óptico (100X, imersão). Para morfometria foi realizada a captura de imagens com o auxílio de uma câmera digital Canon, modelo Powershot A640® acoplada ao microscópio, e foi utilizado o software Zeiss® AxioVision Sample Images. Foram mensuradas 2007 células sanguíneas, sendo 590 eritrócitos, 365 heterófilos, 140 linfócitos, 454 azurófilos, 17 monócitos e 441 trombócitos. Verificou-se que os eritrócitos e trombócitos apresentaram maiores diferenças entre seus diâmetros maiores e menores, conferindo o formato elíptico a estas células. As demais células avaliadas...


Assuntos
Animais , Boidae/sangue , Células Sanguíneas/ultraestrutura , Eritrócitos/ultraestrutura , Linfócitos/ultraestrutura , Plaquetas/ultraestrutura , Brasil , Valores de Referência
10.
Vet. Zoot. ; 28: 1-5, 13 out. 2021. tab
Artigo em Português | VETINDEX | ID: vti-33463

Resumo

Boa constrictor es una especie que se encuentra a menudo en criaderos y zoológicos, además de ser utilizada como animal de compañía. Estos animales son nativos del nuevo mundo y se encuentran desde México hasta el norte de Argentina. El objetivo del presente trabajo es generar valores morfométricos de las células que componen el tejido sanguíneo de individuos de la especie Boa constrictor, identificando sus tamaños promedios, que son importantes para determinar el estado de estas células, siendo principalmente útiles para clasificar las anemias. Los frotis sanguíneos se realizaron con muestras recolectadas de cuatro ejemplares atendidos en el Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), siendo dos machos y dos hembras. Las láminas se tiñeron utilizando la técnica de tinción panótica rápida y se observaron bajo un microscopio óptico (100X, inmersión). Para la morfometría, la captura de imágenes se realizó con la ayuda de una cámara digital Canon acoplada al microscopio, y la medición de las células se realizó con el software Zeiss® AxioVision Sample Images. Se midieron un total de 2007 células de sangre, siendo 590 eritrocitos, 365 heterofilos, 140 linfocitos, 454 azurofilos, 17 monocitos y 441 trombocitos. Se encontró que los eritrocitos y trombocitos mostraron mayores diferencias entre sus diámetros mayores y menores, dando a estas células una forma elíptica...(AU)


Boa constrictor is a species often found in breeding grounds and zoos, in addition to being used as a pet animal. These animals are native to the new world, being found from Mexico to northern Argentina. The aim of the present work is to generate morphometric values of the cells that make up the blood tissue of individuals of the Boa constrictor species, identifying their average sizes, which are important in determining the state of these cells, being mainly useful for classifying anemias. Blood strains were performed with samples collected from four specimens attended at the Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), being two males and two females. The slides were stained using the fast panotic staining technique and observed under an optical microscope (100X, immersion). For morphometry, image capture was performed with the aid of a Canon digital camera attached to the microscope, and the measurement of the cells was done with the Zeiss® AxioVision Sample Images software. A total of 2007 blood cells were measured, being 590 erythrocytes, 365 heterophiles, 140 lymphocytes, 454 azurophils, 17 monocytes and 441 thrombocytes. It was found that erythrocytes and thrombocytes showed greater differences between their larger and smaller diameters, giving these cells an elliptical shape. The other cells evaluated showed similar proportions between the diameters...(AU)


Boa constrictor é uma espécie frequentemente encontrada em criadouros e zoológicos, além de ser utilizada como animal de companhia. Esses animais são nativos do novo mundo, sendo encontrados do México ao norte da Argentina. O trabalho realizado possui como objetivo gerar valores morfométricos das células que compõem o tecido sanguíneo de indivíduos da espécie Boa constrictor, identificando seus tamanhos médios, que são importantes na determinação do estado destas células, sendo úteis principalmente para classificar anemias. Foram realizadas distensões sanguíneas com as amostras coletadas de quatro exemplares atendidos no Núcleo de Estudos e Pesquisas em Animais Selvagens (NEPAS), sendo dois machos e duas fêmeas. As lâminas foram coradas utilizando a técnica de coloração panóptico rápido e observadas ao microscópio óptico (100X, imersão). Para morfometria foi realizada a captura de imagens com o auxílio de uma câmera digital Canon, modelo Powershot A640® acoplada ao microscópio, e foi utilizado o software Zeiss® AxioVision Sample Images. Foram mensuradas 2007 células sanguíneas, sendo 590 eritrócitos, 365 heterófilos, 140 linfócitos, 454 azurófilos, 17 monócitos e 441 trombócitos. Verificou-se que os eritrócitos e trombócitos apresentaram maiores diferenças entre seus diâmetros maiores e menores, conferindo o formato elíptico a estas células. As demais células avaliadas...(AU)


Assuntos
Animais , Células Sanguíneas/ultraestrutura , Boidae/sangue , Eritrócitos/ultraestrutura , Linfócitos/ultraestrutura , Plaquetas/ultraestrutura , Brasil , Valores de Referência
11.
Acta cir. bras ; 36(10): e361007, 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1349866

Resumo

ABSTRACT Purpose: To evaluate the effects of sucralfate enemas in tissue contents of E-cadherin and ?-catenin in an experimental diversion colitis. Methods: Thirty-six male Wistar rats were submitted to a proximal colostomy and a distal mucous fistula. They were allocated into three groups: first group received daily saline enemas (2 mL/day) and the two other groups daily enemas with sucralfate at dosage of 1 or 2 g/kg/day, respectively. Six animals of each group were euthanized after two weeks and six animals after four weeks. The inflammation of the excluded mucosa was evaluated by histological analysis. The oxidative damage was quantified by measurement of malondialdehyde tissue levels. The expression of E-cadherin and ?-catenin was identified by immunohistochemistry, and its contents were quantified by computer-assisted image analysis. Results: Sucralfate enemas reduced inflammation in animals subjected to treatment with 2 g/kg/day by four weeks, and the levels of oxidative damage in mucosa without fecal stream irrespective of concentration and time of intervention. E-cadherin and ?-catenin content increased in segments without fecal stream in those animals subjected to treatment with sucralfate. Conclusions: Sucralfate reduces the inflammation and oxidative stress and increases the tissue content of E-cadherin and ?-catenin in colonic mucosa devoid to the fecal stream.


Assuntos
Humanos , Animais , Ratos , Sucralfato/metabolismo , Cateninas/metabolismo , Caderinas/metabolismo , Ratos Wistar , Estresse Oxidativo , Enema , Mucosa Intestinal/metabolismo
12.
Ars vet ; 36(1): 47-51, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1463516

Resumo

Os glicosímetros (GT) são uma ferramenta muito utilizada no monitoramento da glicemia em cães e o hematócrito (HT) é um dos fatores que pode alterar sua acurácia, possivelmente devido a modificação da viscosidade do sangue, ao impedimento da reação com o plasma na superfície da tira, à alteração da cinética de difusão e/ou da modificação do volume plasmático, resultando em volume insuficiente de plasma para o teste dos GT. Este estudo avaliou o efeito do HT sobre o desempenho de dois GT veterinários para uso em cães em comparação a dois métodos laboratoriais de referência: glicose hexoquinase (Hx) e glicose oxidase (GOx). Ambos os GT apresentaram boa performance em amostras com HT inferior à 37%: GT1 r=0,90 com MLab Hx e r=0,94para GOx. Por sua vez, GT2 apresentou r=0,84 tanto para Hx quanto para GOx. Entretanto, apenas GT1 demonstrou desempenho adequado em amostras com HT (entre 37% e 55% (r=0,90 para Hx) e nenhum GT ofereceu atuação apropriada em amostras com HT superior à 55%. Conclui-se que o resultado apresentado por ambos GT altera de acordo com o hematócrito do animal. Deve-se considerar que pacientes com disglicemias estão frequentemente expostos às alterações clínico-patológicas, e a adoção destes GT específicos como estratégia de monitoramento pode não ser adequada, por isso a importância de outros procedimentos de medição da glicose serem empregados.


Glucometers (GT) are widely used as tools for monitoring blood glucose in dogs and hematocrit (HT) is one of the factors that can change its accuracy, possibly due to the change in blood viscosity, preventing the reaction with plasma on the surface of the strip, alteration of the diffusion kinetics and / or modification of the plasma volume, resulting in insufficient plasma volume for the GT test. This study evaluated the effect of HT on the performance of two veterinary GT for use in dogs compared to two reference laboratory methods (MLab): glucose hexokinase (Hx) and glucose oxidase (GOx). Both GT showed good performance in samples with HT below 37%: GT1 r = 0.90 with MLab Hx and r = 0.94 for GOx. In turn, GT2 presented r = 0.84 for both Hx and GOx. However, only GT1 demonstrated adequate performance in samples with HT between 37% and 55% (r = 0.90 for Hx) and no GT offered adequate performance in samples with HT greater than 55%. It can be concluded that the result presented by both GT changes according to the animal's hematocrit. It should be considered that patients with dysglycemias are frequently exposed to clinical-pathological changes, and the adoption of these specific GT as a monitoring strategy may not be adequate, so the importance of other procedures glucose measurement devices are employed.


Assuntos
Animais , Cães , Glicemia/análise , Hematócrito/veterinária , Índice Glicêmico , Teste de Tolerância a Glucose/veterinária
13.
Ars Vet. ; 36(1): 47-51, 2020. tab
Artigo em Português | VETINDEX | ID: vti-26038

Resumo

Os glicosímetros (GT) são uma ferramenta muito utilizada no monitoramento da glicemia em cães e o hematócrito (HT) é um dos fatores que pode alterar sua acurácia, possivelmente devido a modificação da viscosidade do sangue, ao impedimento da reação com o plasma na superfície da tira, à alteração da cinética de difusão e/ou da modificação do volume plasmático, resultando em volume insuficiente de plasma para o teste dos GT. Este estudo avaliou o efeito do HT sobre o desempenho de dois GT veterinários para uso em cães em comparação a dois métodos laboratoriais de referência: glicose hexoquinase (Hx) e glicose oxidase (GOx). Ambos os GT apresentaram boa performance em amostras com HT inferior à 37%: GT1 r=0,90 com MLab Hx e r=0,94para GOx. Por sua vez, GT2 apresentou r=0,84 tanto para Hx quanto para GOx. Entretanto, apenas GT1 demonstrou desempenho adequado em amostras com HT (entre 37% e 55% (r=0,90 para Hx) e nenhum GT ofereceu atuação apropriada em amostras com HT superior à 55%. Conclui-se que o resultado apresentado por ambos GT altera de acordo com o hematócrito do animal. Deve-se considerar que pacientes com disglicemias estão frequentemente expostos às alterações clínico-patológicas, e a adoção destes GT específicos como estratégia de monitoramento pode não ser adequada, por isso a importância de outros procedimentos de medição da glicose serem empregados.(AU)


Glucometers (GT) are widely used as tools for monitoring blood glucose in dogs and hematocrit (HT) is one of the factors that can change its accuracy, possibly due to the change in blood viscosity, preventing the reaction with plasma on the surface of the strip, alteration of the diffusion kinetics and / or modification of the plasma volume, resulting in insufficient plasma volume for the GT test. This study evaluated the effect of HT on the performance of two veterinary GT for use in dogs compared to two reference laboratory methods (MLab): glucose hexokinase (Hx) and glucose oxidase (GOx). Both GT showed good performance in samples with HT below 37%: GT1 r = 0.90 with MLab Hx and r = 0.94 for GOx. In turn, GT2 presented r = 0.84 for both Hx and GOx. However, only GT1 demonstrated adequate performance in samples with HT between 37% and 55% (r = 0.90 for Hx) and no GT offered adequate performance in samples with HT greater than 55%. It can be concluded that the result presented by both GT changes according to the animal's hematocrit. It should be considered that patients with dysglycemias are frequently exposed to clinical-pathological changes, and the adoption of these specific GT as a monitoring strategy may not be adequate, so the importance of other procedures glucose measurement devices are employed.(AU)


Assuntos
Animais , Cães , Glicemia/análise , Hematócrito/veterinária , Índice Glicêmico , Teste de Tolerância a Glucose/veterinária
14.
Acta sci. vet. (Impr.) ; 48: Pub.1734-Jan. 30, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1458257

Resumo

Background: Changes in glycemic levels can negatively affect the body. Several techniques for the measurement of bloodglucose have been described, but the enzymatic method is considered standard and more accurate in both humans andanimals. The College of American Pathologists recommends the use of portable glucometers (PGs), which are routinelyused in human medicine because this is an easy, relatively inexpensive method that delivers results quickly. The aim ofthis study was to compare the results of the measurement of blood glucose in cattle obtained using portable Accu-Check®glucometer with the enzymatic method (EM), which is still considered standard.Materials, Methods & Results: Thirty crossbred cattle (Bos taurus), male and female, of different ages were evaluated.Through a puncture of the jugular vein, 10 mL of blood was collected, and one drop was immediately used in an AccuChek® PG; the remaining blood was stored in tubes containing sodium fluoride and tubes containing EDTA. The sampleswere packaged and sent to the laboratory for processing. Blood glucose was measured in the sodium fluoride samples using the enzymatic-colorimetric method (EM) employing Labtest® glucose kits with automatic ELISA spectrophotometerreadings. The glycemic values obtained in this study with PG and EM ranged from 62 to 163.3 mg/dL. Mean glucoseconcentrations for the PG and EM methods were 93.53 mg/dL and 94.84 mg/dL, respectively, with no statistical difference(P > 0.05). The glycemia measurement results generated by both tests were normally distributed by the Shapiro–Wilk test(P > 0.05) and equal variances by the Levene test (P > 0.05).


Assuntos
Animais , Bovinos , Análise Química do Sangue/veterinária , Bovinos/sangue , Glicemia , Contagem de Células Sanguíneas/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária
15.
Acta sci. vet. (Online) ; 48: Pub. 1734, 2 junho 2020. tab
Artigo em Português | VETINDEX | ID: vti-29431

Resumo

Background: Changes in glycemic levels can negatively affect the body. Several techniques for the measurement of bloodglucose have been described, but the enzymatic method is considered standard and more accurate in both humans andanimals. The College of American Pathologists recommends the use of portable glucometers (PGs), which are routinelyused in human medicine because this is an easy, relatively inexpensive method that delivers results quickly. The aim ofthis study was to compare the results of the measurement of blood glucose in cattle obtained using portable Accu-Check®glucometer with the enzymatic method (EM), which is still considered standard.Materials, Methods & Results: Thirty crossbred cattle (Bos taurus), male and female, of different ages were evaluated.Through a puncture of the jugular vein, 10 mL of blood was collected, and one drop was immediately used in an AccuChek® PG; the remaining blood was stored in tubes containing sodium fluoride and tubes containing EDTA. The sampleswere packaged and sent to the laboratory for processing. Blood glucose was measured in the sodium fluoride samples using the enzymatic-colorimetric method (EM) employing Labtest® glucose kits with automatic ELISA spectrophotometerreadings. The glycemic values obtained in this study with PG and EM ranged from 62 to 163.3 mg/dL. Mean glucoseconcentrations for the PG and EM methods were 93.53 mg/dL and 94.84 mg/dL, respectively, with no statistical difference(P > 0.05). The glycemia measurement results generated by both tests were normally distributed by the Shapiro–Wilk test(P > 0.05) and equal variances by the Levene test (P > 0.05). (AU)


Assuntos
Animais , Bovinos , Glicemia , Análise Química do Sangue/veterinária , Bovinos/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Contagem de Células Sanguíneas/veterinária
16.
Rev. bras. ciênc. vet ; 27(3): 137-145, jul./set. 2020. il.
Artigo em Português | LILACS, VETINDEX | ID: biblio-1373061

Resumo

Devido aos altos índices de resistência dos parasitas aos diferentes princípios ativos comerciais, novas alternativas de controle vêm sendo estudadas, entre elas a fitoterapia. Essas medidas visam a busca de métodos auxiliares no controle das parasitoses, entretanto, muitos produtos estão disponíveis no mercado e não têm comprovação científica de sua eficácia ou de possíveis efeitos colaterais. O objetivo deste estudo foi avaliar os efeitos hematológicos e hepáticos após a administração de torta de Neem (Azadirachta indica) em ovinos. Foram testadas três dosagens da torta de Neem adicionada ao sal mineral (1, 2 e 4%), administradas por 126 dias para 32 ovinos da raça Lacaune, divididos em quatro grupos sendo três grupos para os diferentes tratamentos e um controle, o qual recebeu somente sal mineral. Amostras de sangue foram colhidas a cada 21 dias para realização do hemograma completo, dosagem de proteína plasmática total e fibrinogênio e avaliação da bioquímica clínica hepática. Nestas mesmas ocasiões, amostras de fezes foram coletadas para a quantificação de ovos por grama de fezes (OPG). Foram observadas diferenças estatísticas entre momentos e grupos para diversas variáveis, porém sem estarem relacionadas à administração de torta de Neem. Os resultados obtidos de hemograma completo, dosagem de proteína plasmática total, fibrinogênio e de bioquímica clínica hepática indicaram que a administração de torta de Neem nas concentrações de 1, 2 e 4%, não interfere nos valores hematológicos, nem sobre a integridade e função hepática de ovinos da raça Lacaune.


Due to high levels of parasite resistance to different commercial active ingredients, new control alternatives are being studied, including the phytotherapy. These measures aim to search for auxiliary methods in the control of parasitic diseases. However, there are many products available in the market and there are no scientific proof of its efficacy. The objective of this study was to evaluate the hematological and hepatic effects following administration of Neem pie (Azadirachta indica) in sheep. Three concentrations of Neem cake was added to the mineral salt (1, 2 and 4%) and administered during 126 days to 32 Lacaune breed sheeps, divided into four groups: three groups for different treatments and a and a control were tested, the ladder receiving only mineral salt. Blood samples were taken every 21 days to perform the complete blood count, serum total plasma protein and fibrinogen and liver biochemical evaluation. In those same times, fecal samples were collected for quantification of eggs per gram of feces (EPG). Statistical differences between times and groups for several variables were observed, but without being related to the administration of Neem pie. The results of complete blood count, measurement of total plasma protein, fibrinogen and hepatic clinical biochemistry indicated that administration of Neem pie at concentrations of 1, 2 and 4%, does not interfere in hematological values, or on the integrity and liver function Lacaune sheep breed.


Assuntos
Animais , Contagem de Ovos de Parasitas/veterinária , Contagem de Células Sanguíneas/veterinária , Ovinos/sangue , Proteínas Sanguíneas/análise , Medicamento Fitoterápico , Testes Hematológicos/veterinária , Testes de Função Hepática/veterinária , Antiparasitários/uso terapêutico
17.
Acta cir. bras. ; 35(11): e351107, 2020. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-30328

Resumo

Purpose: To evaluate the effects of meperidine on fascial healing. Methods: Seventy adult male Sprague-Dawley rats divided into 7 groups with 10 rats in each group. One of these groups was determined as the sham group, 3 of the remaining 6 groups as meperidine groups, and 3 as control groups. These were grouped as 1st, 2nd, and 6th weeks. In the anterior abdominal wall of the rat, the skin was detached and a wound model including the peritoneum was created with a median incision. Mice in the meperidine group were injected with meperidine intraperitoneally (IP) 3 × 20 mg/kg meperidine on postoperative days 0, 1 and 2, and 2 × 20 mg/kg meperidine on postoperative days 3, 4, 5, and 6 after surgical intervention. Similar to the control group, an equal volume of saline was administered, corresponding to the doses. After sacrifice, the midline fascia was used for facial tensile strength measurement, and the other for histopathological analysis. Results: When compared, the meperidine and control groups inflammatory cell density was higher in the 1st week (p < 0.05) in the meperidine group, fibroplasia density was found to be higher at the 2nd week in the meperidine group than the control group (p < 0.05) When the tensile strength in the meperidine and control groups were compared, there was no significant difference (p > 0.05) at each of the three weeks. Conclusion: The application of postoperative systemic meperidine affects positively wound healing in the inflammatory stage and fibroplasia without changing the resistance to traction.(AU)


Assuntos
Animais , Ratos , Meperidina/uso terapêutico , Cicatrização , Fáscia , Inflamação/veterinária
18.
Acta sci., Biol. sci ; 42: e53064, fev. 2020. ilus, tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1460953

Resumo

The research submitted samples from stems and leaf blades from tree genotypes of Pennisetum purpureum called 93-32-02, 92-70-02, and 91-06-02 (EMBRAPA - Dairy cattle) and elephant grass cv. Napier (reference cultivar) to the chemical, anatomical evaluations, and in vitro dry matter digestibility (IVDMD) measurement. The anatomical characteristics of the stems and leaf blades, the chemical composition, and the IVDMD of these genotypes at 70 days of re-growth were correlated. Concerning IVDMD, the data highlighted differences, and the cultivar Napier presented the smallest value. Digital images obtained by light microscopy from cross-section reveal that all the stem and leaf blade have similar structural organization. Quantitative differences were verified mainly in the stem. The leaves displayed differences only in the mesophyll thickness. The genotypes showed higher potential in the rainy season since they had the largest IVDMD when compared to the cultivar Napier.


Assuntos
Pennisetum/anatomia & histologia , Pennisetum/classificação , Pennisetum/genética , Pennisetum/metabolismo , Valor Nutritivo
19.
Arq. bras. med. vet. zootec. (Online) ; 72(5): 1625-1630, Sept.-Oct. 2020. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1131536

Resumo

Magnesium sulphate (MS) and dioctyl sodium sulphosuccinate (DSS) are laxative drugs frequently used for the treatment of impactions. The aim of this study was to compare the effects of MS and DSS in fecal hydration, output and systemic hydration in healthy horses. Five healthy horses received 3 treatments with a 21-day interval. Treatment 1 was performed with administration of 4 liters of warm water; treatment 2: administration of 4 liters of warm water associated with 1g/kg of MS; and treatment 3: administration of 4 liters of warm water associated with 20mg/kg DSS. General and specific physical examination of the digestive system were performed, alongside with packed cell volume and total plasma protein measurement, abdominal ultrasonography and quantification of the amount and hydration of feces, before and 6, 12, 24, 36 and 48 hours after the treatments. No adverse effects were observed. The administration of laxatives promoted greater fecal output and hydration without resulting in systemic dehydration, yet no differences were observed between treatments. The absence of adverse effects of DSS demonstrates the safety of its use as a laxative drug at a dose of 20mg/kg. Studies comparing the effects of the laxative drugs in horses with large colon impaction are needed.(AU)


O sulfato de magnésio (SM) e o dioctil-sulfossuccinato de sódio (DSS) são drogas laxativas frequentemente utilizadas no tratamento da compactação. O objetivo deste estudo foi comparar os efeitos do SM e do DSS na hidratação e eliminação fecal, e na hidratação sistêmica de cavalos saudáveis. Cinco cavalos receberam três tratamentos, com intervalo de 21 dias. O tratamento 1 consistiu na administração de quatro litros de água morna; o tratamento 2: administração de quatro litros de água morna associada a 1g/kg de SM; e o tratamento 3: administração de quatro litros de água morna associada a 20mg/kg de DSS. Foram realizados exames físicos gerais e específicos do trato digestivo, hematócrito e proteína plasmática total, ultrassonografia abdominal e mensuração da quantidade e hidratação das fezes antes e após seis, 12, 24, 36 e 48 horas dos tratamentos. Não foram observados efeitos adversos. A administração de ambos os laxantes promoveu maior produção fecal e hidratação, sem resultar em desidratação sistêmica, entretanto não houve diferença entre os tratamentos. A ausência de efeitos adversos do DSS demonstra a segurança de seu uso como medicamento laxante na dose de 20mg/kg. São necessários estudos que comparem o efeito dos laxantes em equinos portadores de compactação de cólon.(AU)


Assuntos
Animais , Colo/patologia , Ácido Dioctil Sulfossuccínico/uso terapêutico , Laxantes/administração & dosagem , Impacção Fecal/terapia , Cavalos , Sulfato de Magnésio/uso terapêutico , Impacção Fecal/veterinária , Hidratação/métodos
20.
Acta sci. vet. (Impr.) ; 48: Pub.1737-Jan. 30, 2020. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1458260

Resumo

Background: Bovine enzootic haematuria is a chronic, non-infectious disease caused by the ingestion of bracken Pteridiumaquilinum that contain ptaquiloside, substance responsible for the development of neoplasms in combination with bovinepapillomavirus infection. Studies examining metalloproteinases have shown that these enzymes may be useful as prognosticmarkers and many studies have correlated the intratumoural microvessel density and incidence of metastasis in a varietyof cancers, including bladder cancer. This study aimed to evaluate the urothelial morphology and vasculature and quantifythe expression of the enzyme metalloproteinase-2 in the bladder lesions of bovine with enzootic haematuria.Materials, Methods & Results: Forty bladders with macroscopic lesions were subjected to routine histological processing. The material was stained using the Hematoxylin-Eosin (HE) and Weigert’s resorcin-fuchsin staining methods. Themorphometric analysis included the measurement of the total thickness of the urothelium. Microscopically, the lesionswere differentiated as neoplastic or non-neoplastic, and the vascular changes were characterised as vascular proliferation,ectasia, dilation and thickening and the material stained using the Weigert’s resorcin-fuchsin method was used to evaluatesclerosis and the vascular fibroelastosis. The metalloproteinase expression was evaluated using an anti-metalloproteinase-2antibody. The main non-neoplastic lesions found in the urothelium included clear cell metaplasia, dysplasia, hyperplasia,haemorrhage, cystitis cystica and Brunn’s nests and the neoplastic lesions were haemangioma, myxoma, transitional cellcarcinoma, adenocarcinoma, in situ carcinoma and haemangiosarcoma. The urothelium measurements revealed differencesbetween the epithelial thicknesses of bladders with and without neoplasms. The bladder vasculature evaluation revealed...


Assuntos
Animais , Bovinos , Bexiga Urinária/enzimologia , Bexiga Urinária/irrigação sanguínea , Doenças dos Bovinos , Hematúria/veterinária , Bovinos/lesões
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA