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1.
Ciênc. anim. bras. (Impr.) ; 23: e-72715P, 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1404210

Resumo

Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 µM, 20 µM, 50 µM, 100 µM, and 1000 µM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 µM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.


A Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 µM, 20 µM, 50 µM, 100 µM e 1000 µM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50µM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.


Assuntos
Animais , Cães , Neoplasias Ósseas/veterinária , Osteossarcoma/veterinária , Curcumina , Curcuma , Proliferação de Células/efeitos dos fármacos , Técnicas In Vitro/veterinária , Doenças do Cão , Compostos Fitoquímicos
2.
Acta sci. vet. (Impr.) ; 49(supl.1): Pub. 670, 2021. ilus
Artigo em Português | VETINDEX | ID: biblio-1362886

Resumo

Background: Acute lymphoblastic leukemia (ALL) is a malignant neoplasia in which there is proliferation of lymphoid progenitor cells in the bone marrow, blood, and extramedullary sites. This disorder has a fast and progressive development; in dogs, cases of infiltration of ALL cells in the central nervous system (CNS) are uncommon and rare. Diagnosis can be achieved with the help of the clinical history and physical, radiographic, hematological, myelographic, and cerebrospinal fluid (CSF) tests in patients with or without neurological clinical signs. The present report aims to describe a case of ALL and the presence of lymphoblasts in the CSF of a dog with neurological clinical signs. Case: An 8-year-old Lhasa Apso dog was examined at the Veterinary Hospital of Universidade Federal do Paraná, Curitiba campus. At the physical examination, the animal exhibited apathy and paralysis of pelvic limbs, which progressed to tetraplegia. Abdominal palpation revealed presence of hepatosplenomegaly and absence of lymphadenomegaly. No alterations were observed in radiographs of the cervical, thoracic or lumbar spine. A complete blood count revealed presence of non-regenerative anemia (hematocrit = 22%), extreme lymphocytosis (185,229 cells/µL), lymphoblasts at a level of 72% (133,364 cells/µL), and thrombocytopenia (66,000 platelets/µL). The biochemical tests revealed increased alkaline phosphatase (859 IU/L). The levels of alanine aminotransferase, creatinine, urea, total protein, albumin, and globulin were normal. The diagnosis of ALL was achieved with the help of a myelogram. The myelogram findings included 39% of mature lymphocytes and 59% of lymphoblasts exhibiting large size, spherical shape, poorly delimited borders, with a high nucleus/cytoplasm ratio, marked cytoplasmic basophilia, and 2 to 3 evident nucleoli; metarubricytes (1%) and promyelocytes (0.6%) were also observed. The CSF contained an increased number of nucleated cells (27 cells/µL) comprising lymphocytes (43%), macrophages (33%), and segmented neutrophils (24%). Of the 11.6 lymphocytes per µL of CSF, 8.1 were lymphoblasts, which indicates infiltration of ALL cells in the CNS. The animal died one day after collection of bone barrow and CSF. Discussion: Relevant alterations observed in this case included the neurological signs caused by the infiltration of neoplastic cells in the CNS, severe leukocytosis and lymphocytosis, with large amounts of lymphoblasts in the blood and predominance of lymphoblasts in the bone marrow, which are alterations typically found in ALL. The animal also exhibited non-regenerative anemia and thrombocytopenia, which were secondary to infiltration of leukemic cells in the bone marrow. The CSF exhibited pleocytosis (27 cells/ µL), and 30% of the cells observed were lymphoblasts. Lymphoblast infiltration in the CNS of leukemic dogs is rare, and other studies have reported absence of neurological signs or neurological signs different from those observed in the present study. CSF analysis in indicated in cases of leukemia to assess leukemic cell infiltration in the CNS. In the case reported here, the plasma level of alkaline phosphatase was increased (859 IU/L) as a consequence of hepatomegaly and hepatic cholestasis. ALL is a very aggressive, proliferative neoplasia, and the resulting lymphoblasts infiltrated the CNS of the animal. In cases of ALL, performing complete blood count, myelogram, and CSF analysis is indicated whether the patients exhibit neurological signs or not.(AU)


Assuntos
Animais , Cães , Sistema Nervoso Central/patologia , Líquido Cefalorraquidiano , Leucemia-Linfoma Linfoblástico de Células Precursoras/veterinária , Invasividade Neoplásica , Linfócitos , Mielografia/veterinária , Transtornos Linfoproliferativos/veterinária
3.
Acta sci. vet. (Impr.) ; 49(suppl.1): Pub.650-Jan 4, 2021. ilus
Artigo em Português | VETINDEX | ID: biblio-1458509

Resumo

Background: Fibrosing osteomyelitis is a chronic inflammatory process caused by infectious agents that lead to the destructionand replacement of bone tissue by fibroblasts. The diagnosis is based especially on histopathological and bacterial culture. Incases where extensive and irreversible injuries are observed, surgical treatment may be indicated. The objective of this work isto report the clinical, radiographic, histopathological, and microbiological aspects of a cat presenting fibrosing osteomyelitis.Case: A 10-year-old male feline, no defined breed, weighing 3.9 kg was referred to one Private Veterinary Clinic of Fortaleza,CE, Brazil with a history of left mandibular enlargement, presenting dysphagia, sialorrhea, an increase in firm consistencyalong of the left mandibular body, temporomandibular arthralgia and decreased joint motion range. On cranium radiograph,signs of proliferative osteopathy of irregular contours were observed in the branch and body of the left mandible and extending to the rostral region of the right mandible, suggesting a neoplastic process. After anesthesia, for better assessment ofthe oral cavity, a sample was collected by incisional biopsy, however, the histopathological result was nonspecific. In viewof the inconclusive condition, it was decided to perform left hemimandibulectomy combined with right partial mandibulectomy. Tissue samples were obtained and sent for microbiological and histopathological analyses. The last test revealedan inflammatory reaction consisting of neutrophils and plasma cells, associated with a large amount of fibrous connectivetissue, multifocal bacterial aggregates, necrosis and bone resorption. Based on the findings, the diagnosis of chronic bacterial osteomyelitis was concluded. The microbiological culture demonstrated the growth of the bacterium Pseudomonasaeruginosa, with sensitivity to cefovecin, which was administered to the treatment in....


Assuntos
Masculino , Animais , Gatos , Gatos/cirurgia , Osteomielite/veterinária , Osteotomia Mandibular/veterinária , Infecções Bacterianas/veterinária , Infecções por Pseudomonas/veterinária , Pseudomonas aeruginosa
4.
Acta sci. vet. (Online) ; 49(suppl.1): Pub. 650, 5 jun. 2021. ilus
Artigo em Português | VETINDEX | ID: vti-764707

Resumo

Background: Fibrosing osteomyelitis is a chronic inflammatory process caused by infectious agents that lead to the destructionand replacement of bone tissue by fibroblasts. The diagnosis is based especially on histopathological and bacterial culture. Incases where extensive and irreversible injuries are observed, surgical treatment may be indicated. The objective of this work isto report the clinical, radiographic, histopathological, and microbiological aspects of a cat presenting fibrosing osteomyelitis.Case: A 10-year-old male feline, no defined breed, weighing 3.9 kg was referred to one Private Veterinary Clinic of Fortaleza,CE, Brazil with a history of left mandibular enlargement, presenting dysphagia, sialorrhea, an increase in firm consistencyalong of the left mandibular body, temporomandibular arthralgia and decreased joint motion range. On cranium radiograph,signs of proliferative osteopathy of irregular contours were observed in the branch and body of the left mandible and extending to the rostral region of the right mandible, suggesting a neoplastic process. After anesthesia, for better assessment ofthe oral cavity, a sample was collected by incisional biopsy, however, the histopathological result was nonspecific. In viewof the inconclusive condition, it was decided to perform left hemimandibulectomy combined with right partial mandibulectomy. Tissue samples were obtained and sent for microbiological and histopathological analyses. The last test revealedan inflammatory reaction consisting of neutrophils and plasma cells, associated with a large amount of fibrous connectivetissue, multifocal bacterial aggregates, necrosis and bone resorption. Based on the findings, the diagnosis of chronic bacterial osteomyelitis was concluded. The microbiological culture demonstrated the growth of the bacterium Pseudomonasaeruginosa, with sensitivity to cefovecin, which was administered to the treatment in....(AU)


Assuntos
Animais , Masculino , Gatos , Osteotomia Mandibular/veterinária , Gatos/cirurgia , Osteomielite/veterinária , Infecções Bacterianas/veterinária , Pseudomonas aeruginosa , Infecções por Pseudomonas/veterinária
5.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1471184

Resumo

Osteitis deformans (Pagets disease) is a chronic bone disorder characterized by excessive osteoclast-mediated bone resorption followed by new bone formation. The present paper reports this condition in an 18-year-old captive golden lancehead (Bothrops insularis) from Brazil. This patient initially exhibited anorexia and swelling in the middle third of the spine associated with locomotor disability. For diagnosis, radiography, ultrasound, computed tomography, cytology, and microbiological culture were performed. Diagnostic imaging showed bone changes, vertebral fusion, and bone proliferation. Cytology revealed blood cells how toxic heterophiles, reactive monocytes, young red blood cells, and polychromasia compatible with an infectious process. A bacterial culture identified an ampicillin-susceptible strain of Enterococcus faecalis. Antibiotic treatment was promptly started, but the snake died 25 days later. Histopathologically, the bone tissue showed a generalized thickening of the vertebral trabeculae. For the first time, the presence of E. faecalis associated with the development of osteitis deformans in snakes was presented.


Osteíte deformante (Doença de Paget) é um distúrbio ósseo crônico caracterizado por reabsorção óssea excessiva mediada por osteoclastos, seguida por nova formação óssea. O presente trabalho relata essa condição em uma serpente jararaca-ilhoa (Bothrops insularis) do Brasil de 18 anos. O paciente apresentou inicialmente anorexia e um inchaço no primeiro terço médio da coluna associado com a incapacidade locomotora. O diagnóstico foi estabelecido com o apoio de radiografia, ultrassonografia, tomografia computadorizada, citologia e cultura microbiológica. O diagnóstico por imagem mostrou alterações ósseas, fusão de vértebras e proliferação óssea. A citologia mostrou células sanguíneas como heterófílos tóxicos, monócitos reativos, células sanguíneas jovens e policromasia compatíveis com um processo infeccioso. A cultura bacteriana identificou uma cepa de Enterococcus faecalis suscetível à ampicilina. O tratamento com antibióticos foi iniciado imediatamente, mas a serpente morreu 25 dias depois. Histopatologicamente, o tecido ósseo mostrou um espessamento generalizado das trabéculas vertebrais. Portanto, foi demonstrado pela primeira vez a presença de E. faecalis associada ao desenvolvimento de osteíte deformante em uma serpente.

6.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1471201

Resumo

Osteitis deformans (Pagets disease) is a chronic bone disorder characterized by excessive osteoclast-mediated bone resorption followed by new bone formation. The present paper reports this condition in an 18-year-old captive golden lancehead (Bothrops insularis) from Brazil. This patient initially exhibited anorexia and swelling in the middle third of the spine associated with locomotor disability. For diagnosis, radiography, ultrasound, computed tomography, cytology, and microbiological culture were performed. Diagnostic imaging showed bone changes, vertebral fusion, and bone proliferation. Cytology revealed blood cells how toxic heterophiles, reactive monocytes, young red blood cells, and polychromasia compatible with an infectious process. A bacterial culture identified an ampicillin-susceptible strain of Enterococcus faecalis. Antibiotic treatment was promptly started, but the snake died 25 days later. Histopathologically, the bone tissue showed a generalized thickening of the vertebral trabeculae. For the first time, the presence of E. faecalis associated with the development of osteitis deformans in snakes was presented.


Osteíte deformante (Doença de Paget) é um distúrbio ósseo crônico caracterizado por reabsorção óssea excessiva mediada por osteoclastos, seguida por nova formação óssea. O presente trabalho relata essa condição em uma serpente jararaca-ilhoa (Bothrops insularis) do Brasil de 18 anos. O paciente apresentou inicialmente anorexia e um inchaço no primeiro terço médio da coluna associado com a incapacidade locomotora. O diagnóstico foi estabelecido com o apoio de radiografia, ultrassonografia, tomografia computadorizada, citologia e cultura microbiológica. O diagnóstico por imagem mostrou alterações ósseas, fusão de vértebras e proliferação óssea. A citologia mostrou células sanguíneas como heterófílos tóxicos, monócitos reativos, células sanguíneas jovens e policromasia compatíveis com um processo infeccioso. A cultura bacteriana identificou uma cepa de Enterococcus faecalis suscetível à ampicilina. O tratamento com antibióticos foi iniciado imediatamente, mas a serpente morreu 25 dias depois. Histopatologicamente, o tecido ósseo mostrou um espessamento generalizado das trabéculas vertebrais. Portanto, foi demonstrado pela primeira vez a presença de E. faecalis associada ao desenvolvimento de osteíte deformante em uma serpente.

7.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 57(4): e163926, 2020. ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1348182

Resumo

Osteitis deformans (Paget's disease) is a chronic bone disorder characterized by excessive osteoclast-mediated bone resorption followed by new bone formation. The present paper reports this condition in an 18-year-old captive golden lancehead (Bothrops insularis) from Brazil. This patient initially exhibited anorexia and swelling in the middle third of the spine associated with locomotor disability. For diagnosis, radiography, ultrasound, computed tomography, cytology, and microbiological culture were performed. Diagnostic imaging showed bone changes, vertebral fusion, and bone proliferation. Cytology revealed blood cells how toxic heterophiles, reactive monocytes, young red blood cells, and polychromasia compatible with an infectious process. A bacterial culture identified an ampicillin-susceptible strain of Enterococcus faecalis. Antibiotic treatment was promptly started, but the snake died 25 days later. Histopathologically, the bone tissue showed a generalized thickening of the vertebral trabeculae. For the first time, the presence of E. faecalisassociated with the development of osteitis deformans in snakes was presented.(AU)


Osteíte deformante (Doença de Paget) é um distúrbio ósseo crônico caracterizado por reabsorção óssea excessiva mediada por osteoclastos, seguida por nova formação óssea. O presente trabalho relata essa condição em uma serpente jararaca-ilhoa (Bothrops insularis) do Brasil de 18 anos. O paciente apresentou inicialmente anorexia e um inchaço no primeiro terço médio da coluna associado com a incapacidade locomotora. O diagnóstico foi estabelecido com o apoio de radiografia, ultrassonografia, tomografia computadorizada, citologia e cultura microbiológica. O diagnóstico por imagem mostrou alterações ósseas, fusão de vértebras e proliferação óssea. A citologia mostrou células sanguíneas como heterófílos tóxicos, monócitos reativos, células sanguíneas jovens e policromasia compatíveis com um processo infeccioso. A cultura bacteriana identificou uma cepa de Enterococcus faecalis suscetível à ampicilina. O tratamento com antibióticos foi iniciado imediatamente, mas a serpente morreu 25 dias depois. Histopatologicamente, o tecido ósseo mostrou um espessamento generalizado das trabéculas vertebrais. Portanto, foi demonstrado pela primeira vez a presença de E. faecalis associada ao desenvolvimento de osteíte deformante em uma serpente.(AU)


Assuntos
Animais , Osteíte Deformante/patologia , Osso e Ossos , Tomografia Computadorizada por Raios X , Ultrassonografia , Enterococcus faecalis , Bothrops/microbiologia
8.
Braz. j. vet. res. anim. sci ; 57(4): e163926, 2020. ilus
Artigo em Inglês | VETINDEX | ID: vti-33072

Resumo

Osteitis deformans (Paget's disease) is a chronic bone disorder characterized by excessive osteoclast-mediated bone resorption followed by new bone formation. The present paper reports this condition in an 18-year-old captive golden lancehead (Bothrops insularis) from Brazil. This patient initially exhibited anorexia and swelling in the middle third of the spine associated with locomotor disability. For diagnosis, radiography, ultrasound, computed tomography, cytology, and microbiological culture were performed. Diagnostic imaging showed bone changes, vertebral fusion, and bone proliferation. Cytology revealed blood cells how toxic heterophiles, reactive monocytes, young red blood cells, and polychromasia compatible with an infectious process. A bacterial culture identified an ampicillin-susceptible strain of Enterococcus faecalis. Antibiotic treatment was promptly started, but the snake died 25 days later. Histopathologically, the bone tissue showed a generalized thickening of the vertebral trabeculae. For the first time, the presence of E. faecalisassociated with the development of osteitis deformans in snakes was presented.(AU)


Osteíte deformante (Doença de Paget) é um distúrbio ósseo crônico caracterizado por reabsorção óssea excessiva mediada por osteoclastos, seguida por nova formação óssea. O presente trabalho relata essa condição em uma serpente jararaca-ilhoa (Bothrops insularis) do Brasil de 18 anos. O paciente apresentou inicialmente anorexia e um inchaço no primeiro terço médio da coluna associado com a incapacidade locomotora. O diagnóstico foi estabelecido com o apoio de radiografia, ultrassonografia, tomografia computadorizada, citologia e cultura microbiológica. O diagnóstico por imagem mostrou alterações ósseas, fusão de vértebras e proliferação óssea. A citologia mostrou células sanguíneas como heterófílos tóxicos, monócitos reativos, células sanguíneas jovens e policromasia compatíveis com um processo infeccioso. A cultura bacteriana identificou uma cepa de Enterococcus faecalis suscetível à ampicilina. O tratamento com antibióticos foi iniciado imediatamente, mas a serpente morreu 25 dias depois. Histopatologicamente, o tecido ósseo mostrou um espessamento generalizado das trabéculas vertebrais. Portanto, foi demonstrado pela primeira vez a presença de E. faecalis associada ao desenvolvimento de osteíte deformante em uma serpente.(AU)


Assuntos
Animais , Osteíte Deformante/patologia , Osso e Ossos , Tomografia Computadorizada por Raios X , Ultrassonografia , Enterococcus faecalis , Bothrops/microbiologia
9.
Ci. Rural ; 50(8): e20191007, July 3, 2020. ilus
Artigo em Inglês | VETINDEX | ID: vti-746128

Resumo

Prostatic carcinomas in are aggressive neoplasms and bone metastases may occur; however, hypertrophic osteopathy associated with that condition is poorly documented. A ten-year-old, neutered male, mixed breed dog had a history of lameness and volume increase in the left pelvic limb. On radiographic examination, a lytic bone mass was observed in the left metatarsus, as well as a diffuse proliferative periosteal reaction in several bones of the appendicular skeleton, in addition to radiopaque nodular structures in all lung lobes. A presumptive diagnosis of primary bone neoplasia with pulmonary metastases and hypertrophic osteopathy was established and chemotherapy treatment was started. However, there was no satisfactory clinical response, and euthanasia was ellected. At necropsy, there was moderate enlargement of the prostate gland. The gland was firm and whitish, with a multilobulated aspect. Several similar masses were observed in the right kidney, lungs, mediastinal lymph nodes, and multiple bones of the appendicular skeleton. These bones also presented evident diffuse periosteal reaction. Histological examination revealed a metastatic prostatic carcinoma with bone involvement and hypertrophic osteopathy. This report is an unusual case of metastatic prostatic carcinoma in association with hypertrophic osteopathy and concomitant bone metastases.(AU)


Os carcinomas prostáticos em cães são neoplasmas agressivos e as metástases ósseas podem ocorrer, entretanto a associação com osteopatia hipertrófica é pouco relatada. Um canino, macho, sem raça definida de 10 anos de idade, com histórico de claudicação e aumento de volume em membro pélvico esquerdo, apresentou no exame radiográfico uma massa óssea, lítica em metatarso esquerdo, bem como reação periosteal proliferativa difusa em diversos ossos do esqueleto apendicular além de estruturas nodulares, radiopacas em todos os lobos pulmonares. Realizou-se o diagnóstico presuntivo de neoplasia óssea primaria com metástases pulmonares e osteopatia hipertrófica e iniciou-se o tratamento quimioterápico. Todavia, não houve resposta clínica satisfatória, optando-se pela eutanásia. Na necropsia foi constatado aumento de volume moderado da próstata, com aspecto multilobulado, ao corte firme e brancacento. Diversas massas similares foram observadas no rim direito, pulmões, linfonodos mediastínicos e em vários ossos do esqueleto apendicular, além de evidente reação periosteal difusa. A avaliação histológica revelou um carcinoma prostático metastático com envolvimento ósseo, bem como osteopatia hipertrófica pulmonar. Esse relato é um caso incomum de carcinoma prostático metastático devido a associação com osteopatia hipertrófica pulmonar e as metástases ósseas concomitantes.(AU)


Assuntos
Animais , Masculino , Cães , Doenças do Cão , Metástase Neoplásica , Carcinoma/veterinária , Hipertrofia/veterinária , Neoplasias da Próstata/veterinária
10.
Pesqui. vet. bras ; 40(11): 837-841, Nov. 2020.
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1155026

Resumo

Two outbreaks of cobalt deficiency in beef cattle were diagnosed in Midwestern Brazil. We discuss the clinical, epidemiological, pathological features, therapeutic measures, and impact aspects of the production system associated with these outbreaks occurring outbreaks in two farms of extensive cattle raising-system in the state of Mato Grosso do Sul. Seven affected cattle were euthanized and necropsied. Tissues for histopathology and microelements dosage were secured. At Farm A, 3100 cattle of all ages got sick, and 396 died; at Farm B, 148 were affected, and 110 died. In both farms, cattle were fed the same mineral supplement. The main clinical signs were weight loss and weakness, even though a good supply of forage was available in the paddocks. Many cattle stop grazing and chew at tree barks, wood chips from fence posts, and bones. In addition to the deaths, there was a compromised growth, and the reproductive rates fell sharply. The necropsied cattle were thin, with rough hair coat and pale mucous membranes. The liver was diffusely orange and showed a lobular pattern. The bone marrow was gelatinous and diffusely yellow. Histological changes included hemosiderosis in the liver and spleen, hepatocellular vacuolar degeneration, and myeloid and erythroid hypoplasia of the bone marrow. In the white matter of four cattle's brains, the myelin sheath was markedly distended (spongy degeneration). Proliferative parasitic abomasitis was observed in three cattle. The presumptive diagnosis was based on the association of the clinical picture, the necropsy findings, and the ruling out of other possible causes. The diagnosis was confirmed by the favorable response to treatment with cobalt and vitamin B12 orally and by mineral supplementation.(AU)


Dois surtos de deficiência de cobalto em bovinos de corte foram diagnosticados. Os aspectos clínicos, epidemiológicos, anatomopatológicos, terapêuticos e impactos no sistema de produção são descritos e discutidos. Os surtos ocorreram em duas fazendas de criação extensiva estado de Mato Grosso do Sul, Centro-Oeste brasileiro. Sete bovinos afetados foram eutanasiados e necropsiados. Na necropsia foram colhidas amostras para exames histopatológicos e dosagem de microelementos. Na Fazenda A, 3100 bovinos de todas as idades adoeceram e 396 morreram e na Fazenda B, 148 bovinos adoeceram e 110 morreram. Ambas as fazendas utilizavam o mesmo suplemento mineral. Os principais sinais clínicos observados foram emagrecimento e fraqueza, apesar da boa oferta de forragem nos piquetes, muitos bovinos deixaram de pastejar e comiam cascas de árvores, madeira das porteiras e ossos. Além das mortes, tiveram crescimento comprometido e os índices reprodutivos tiveram queda acentuada. Os bovinos necropsiados estavam magros, com os pelos arrepiados e mucosas pálidas. O fígado estava difusamente alaranjado e com evidenciação do padrão lobular. A medula óssea estava de consistência gelatinosa e difusamente amarelada. Alterações histológicas incluíam degeneração vacuolar hemossiderose que era moderada no fígado e marcada no baço. Hipoplasia mieloide e eritoide era vista na medula óssea. Na substância branca do encéfalo de quatro bovinos, a bainha de mielina estava marcadamente distendida (degeneração esponjosa). Abomasite parasitária proliferativa foi observada em três bovinos. O diagnóstico presuntivo baseou-se na associação do quadro clínico, nos achados de necropsia e na eliminação de outras possíveis causas. O diagnóstico foi confirmado pela resposta favorável ao tratamento com cobalto e vitamina B12 por via oral, e a suplementação mineral.(AU)


Assuntos
Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Cobalto/deficiência , Deficiência de Minerais
11.
Pesqui. vet. bras ; 40(11): 837-841, Nov. 2020.
Artigo em Inglês | VETINDEX | ID: vti-33051

Resumo

Two outbreaks of cobalt deficiency in beef cattle were diagnosed in Midwestern Brazil. We discuss the clinical, epidemiological, pathological features, therapeutic measures, and impact aspects of the production system associated with these outbreaks occurring outbreaks in two farms of extensive cattle raising-system in the state of Mato Grosso do Sul. Seven affected cattle were euthanized and necropsied. Tissues for histopathology and microelements dosage were secured. At Farm A, 3100 cattle of all ages got sick, and 396 died; at Farm B, 148 were affected, and 110 died. In both farms, cattle were fed the same mineral supplement. The main clinical signs were weight loss and weakness, even though a good supply of forage was available in the paddocks. Many cattle stop grazing and chew at tree barks, wood chips from fence posts, and bones. In addition to the deaths, there was a compromised growth, and the reproductive rates fell sharply. The necropsied cattle were thin, with rough hair coat and pale mucous membranes. The liver was diffusely orange and showed a lobular pattern. The bone marrow was gelatinous and diffusely yellow. Histological changes included hemosiderosis in the liver and spleen, hepatocellular vacuolar degeneration, and myeloid and erythroid hypoplasia of the bone marrow. In the white matter of four cattle's brains, the myelin sheath was markedly distended (spongy degeneration). Proliferative parasitic abomasitis was observed in three cattle. The presumptive diagnosis was based on the association of the clinical picture, the necropsy findings, and the ruling out of other possible causes. The diagnosis was confirmed by the favorable response to treatment with cobalt and vitamin B12 orally and by mineral supplementation.(AU)


Dois surtos de deficiência de cobalto em bovinos de corte foram diagnosticados. Os aspectos clínicos, epidemiológicos, anatomopatológicos, terapêuticos e impactos no sistema de produção são descritos e discutidos. Os surtos ocorreram em duas fazendas de criação extensiva estado de Mato Grosso do Sul, Centro-Oeste brasileiro. Sete bovinos afetados foram eutanasiados e necropsiados. Na necropsia foram colhidas amostras para exames histopatológicos e dosagem de microelementos. Na Fazenda A, 3100 bovinos de todas as idades adoeceram e 396 morreram e na Fazenda B, 148 bovinos adoeceram e 110 morreram. Ambas as fazendas utilizavam o mesmo suplemento mineral. Os principais sinais clínicos observados foram emagrecimento e fraqueza, apesar da boa oferta de forragem nos piquetes, muitos bovinos deixaram de pastejar e comiam cascas de árvores, madeira das porteiras e ossos. Além das mortes, tiveram crescimento comprometido e os índices reprodutivos tiveram queda acentuada. Os bovinos necropsiados estavam magros, com os pelos arrepiados e mucosas pálidas. O fígado estava difusamente alaranjado e com evidenciação do padrão lobular. A medula óssea estava de consistência gelatinosa e difusamente amarelada. Alterações histológicas incluíam degeneração vacuolar hemossiderose que era moderada no fígado e marcada no baço. Hipoplasia mieloide e eritoide era vista na medula óssea. Na substância branca do encéfalo de quatro bovinos, a bainha de mielina estava marcadamente distendida (degeneração esponjosa). Abomasite parasitária proliferativa foi observada em três bovinos. O diagnóstico presuntivo baseou-se na associação do quadro clínico, nos achados de necropsia e na eliminação de outras possíveis causas. O diagnóstico foi confirmado pela resposta favorável ao tratamento com cobalto e vitamina B12 por via oral, e a suplementação mineral.(AU)


Assuntos
Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Cobalto/deficiência , Deficiência de Minerais
12.
Ci. Anim. bras. ; 20: e-34543, Aug. 22, 2019. ilus, tab, graf
Artigo em Português | VETINDEX | ID: vti-21875

Resumo

O fator de crescimento transformador-β (TGF-β), um mediador do crescimento prostático, induz a angiogênese e inibe a proliferação celular. Neste estudo, esse marcador foi utilizado com o objetivo de avaliar sua imunomarcação no tecido normal e com lesões proliferativas benignas, pré-neoplásicas e neoplásicas da próstata canina. Para isso, foram selecionadas 54 glândulas com histomorfologia normal, hiperplasia prostática benigna (HPB) epitelial, HPB estromal, atrofia inflamatória proliferativa (PIA), neoplasia intraepitelial prostática (PIN) e carcinoma, utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à técnica de imunoistoquímica com o anticorpo anti-TGF-β, sendo avaliada a intensidade de imunomarcação nas células epiteliais e estromais. Houve imunomarcação de TGF-β no tecido normal e naqueles com lesões proliferativas. Maior imunomarcação de TGF-β foi constatada nas células do tecido prostático normal e com HPB, enquanto as células prostáticas com PIA, PIN e carcinoma exibiram menor imunomarcação dessa citocina, o que sugere a ação do TGF-β na manutenção da homeostase do tecido normal e com lesão proliferativa benigna e na progressão das lesões proliferativas pré-malignas e malignas da próstata canina.(AU)


The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate.(AU)


Assuntos
Animais , Masculino , Cães , Fator de Crescimento Transformador beta/análise , Doenças Prostáticas/diagnóstico , Doenças Prostáticas/veterinária , Carcinoma/diagnóstico , Neoplasia Prostática Intraepitelial/diagnóstico , Neoplasia Prostática Intraepitelial/veterinária , Imuno-Histoquímica/veterinária
13.
Ciênc. anim. bras. (Impr.) ; 20: e, 2019. ilus, tab, graf
Artigo em Português | VETINDEX | ID: biblio-1473688

Resumo

O fator de crescimento transformador-β (TGF-β), um mediador do crescimento prostático, induz a angiogênese e inibe a proliferação celular. Neste estudo, esse marcador foi utilizado com o objetivo de avaliar sua imunomarcação no tecido normal e com lesões proliferativas benignas, pré-neoplásicas e neoplásicas da próstata canina. Para isso, foram selecionadas 54 glândulas com histomorfologia normal, hiperplasia prostática benigna (HPB) epitelial, HPB estromal, atrofia inflamatória proliferativa (PIA), neoplasia intraepitelial prostática (PIN) e carcinoma, utilizadas para a confecção de um bloco de microarranjo tecidual (Tissue Microarray - TMA). As lâminas de TMA foram submetidas à técnica de imunoistoquímica com o anticorpo anti-TGF-β, sendo avaliada a intensidade de imunomarcação nas células epiteliais e estromais. Houve imunomarcação de TGF-β no tecido normal e naqueles com lesões proliferativas. Maior imunomarcação de TGF-β foi constatada nas células do tecido prostático normal e com HPB, enquanto as células prostáticas com PIA, PIN e carcinoma exibiram menor imunomarcação dessa citocina, o que sugere a ação do TGF-β na manutenção da homeostase do tecido normal e com lesão proliferativa benigna e na progressão das lesões proliferativas pré-malignas e malignas da próstata canina.


The transforming growth factor-β (TGF-β), a mediator of prostatic growth induces angiogenesis and inhibits cell proliferation. It was used in this study in order to evaluate its expression in normal prostatic tissue and those with benign proliferative lesions, pre-malignant and malignant prostatic diseases. A total of 54 glands with normal histomorphology, epithelial benign prostatic hyperplasia (BPH), stromal BPH, prostatic inflammatory atrophy (PIA), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma were selected and used in the tissue microarray block (TMA). The TMA slides were subjected to the immunohistochemistry with anti-TGF-β and its staining intensity in epithelial and stromal cells was evaluated. There was TGF-β immunostaining by both normal tissues and those with proliferative lesions. Higher TGF-β immunostaining was observed in cells from normal prostatic tissues and with HPB, whereas prostatic cells with PIA, PIN and carcinoma exhibited lower immunostaining of this cytokine, suggesting the action of TGF-β in the maintenance of normal tissue homeostasis and with benign proliferative lesion and in the progression of pre-malignant and malignant proliferative lesions of the canine prostate.


Assuntos
Masculino , Animais , Cães , Carcinoma/diagnóstico , Doenças Prostáticas/diagnóstico , Doenças Prostáticas/veterinária , Fator de Crescimento Transformador beta/análise , Neoplasia Prostática Intraepitelial/diagnóstico , Neoplasia Prostática Intraepitelial/veterinária , Imuno-Histoquímica/veterinária
14.
Acta sci. vet. (Impr.) ; 41(supl.1): Pub. 35, 2013. ilus
Artigo em Português | VETINDEX | ID: biblio-1372632

Resumo

Background: Chondrosarcoma is a malignant neoplasm of mesenchymal origin that generally develops slowly and has a low incidence of metastasis. It was related in dogs, but rarely in small or giant breeds. The most common site of skeletal chondrossarcoma in dogs is the nasal cavity. The lungs are the most common site of metastatic disease, but other organs may be affected. Chondrosarcoma may be classified into primary or secondary according to location in bone, and histologically into mesenchymal and myxoid subtypes. Thus, this study reports the evolution of an uncommon case of chondrossarcoma in a dog's tibia. Case: A 1.4-year-old dog, 58 kg intact-male Brazilian Mastiff was admitted to the veterinary hospital due to lameness and swelling of the stifle region present for 20 days. Radiographic examination showed lytic bone lesions in the proximal third of the right tibia with periosteal reaction, suggestive of bone tumor. Cytological examination revealed inflammatory process indicative of acute periostitis. The clinical signs improved after treatment with nonsteroidal anti-inflammatory (meloxicam, 0.1 mg/kg orally every 24 h) and analgesic (tramadol hydrochloride, 2 mg/kg orally every 8 h) drugs. However, eight months after the initial presentation, the dog had a recurrence of the lameness, and increase in volume and crepitus on palpation of the right knee. Radiographic examination showed cystic lesion located in proximal tibial epiphysis. After curettage of the lesion, the defect was fi lled with calcium phosphate biomaterial. Cephalexin (30 mg/kg every 12 h orally for 10 days), carprofen (4.4 mg/kg orally every 24 h for 15 days) and chondroitin sulphate (1000 mg orally each 12 h per 30 days) were prescribed postoperatively. The animal showed improvement. At one year after surgery, the dog was presented due progressive weight loss and intermittent lameness of the right hind limb observed for approximately two months. On palpation of the right knee was painful sensitivity and it had a cutaneous nodule three inches. Radiographic examination showed extensive radiolucent cystic area with bone remodeling and periosteal proliferation of the medial aspect of the proximal third of the right tibia. Computed Tomography (CT) demonstrated bone destruction in the proximal portion of the tibia with areas of bone lysis and proliferative irregular reaction, irregular hyperdensity in the medullary region of the right tibia extending to the distal portion of the tibial tuberosity associated with the presence of proliferative periosteal reaction adjacent the bony surfaces, and atrophy of muscles of right hindlimb. Both exams suggested a bone tumor, and bone biopsy was done. The diagnosis was chondrosarcoma. The owner did not authorize the hind limb amputation. Discussion: The diagnosis of chondrosarcoma is based on clinical signs, orthopedic examination findings, radiographic and CT appearance, and cytology. However, definite diagnosis may be obtained only by histopathological examination. Fine needle aspiration samples may contain few cells, and presence of inflammatory process may interfere in diagnosis, as observed in the present case. The tumor is resistant to radiotherapy and chemotherapy. However, surgical removal can be curative, depending upon the location of the chondrosarcoma. Unfortunately, the owner was reluctant to proceed with limb amputation, and the dog was euthanatized after three months of the last evaluation.


Assuntos
Animais , Masculino , Cães , Tíbia/fisiopatologia , Neoplasias Ósseas/diagnóstico por imagem , Condrossarcoma/veterinária , Doenças do Cão/diagnóstico por imagem , Cães
15.
Acta sci. vet. (Impr.) ; 41(supl.1): Pub. 19, 2013. tab, ilus
Artigo em Português | VETINDEX | ID: biblio-1372656

Resumo

Background: Leukemias are malign neoplasias of the hematopoietic precursor cells from the bone marrow. These neoplastic cells may or may not reach the blood circulation. Leukemias are classified as lymphoid or myeloid depending on the leukocyte cell lineage affected. In addition, leukemias are classified as either acute or chronic based on their cellular differentiation and proliferative aggressiveness. The leukemias from lymphoid cells that affect dogs include acute lymphoblastic leukemia and chronic lymphocytic leukemia. This paper describes the clinical and laboratorial findings of a case of acute lymphoblastic leukemia in a female dog. Case: A seven-year-old female mixed-breed dog was referred to the Veterinary Hospital of the Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, State of Rio Grande do Norte, Brazil, for apathy, anorexia, weakness and weight loss. The clinical examination confirmed the apathy and weakness. The mucous membranes were pale, the body temperature was 40.5°C, the heart rate was 120 bpm, the capillary refill time was 2 s, and no abnormalities were found on auscultation. Diagnostic laboratory tests were completed, including a serum test for canine leishmaniasis, a serum biochemistry panel, a complete blood cell count, and a bone marrow analysis. The canine leishmaniasis serology (enzyme-linked immunosorbent assay and indirect fluorescent-antibody test) was negative. The serum biochemistry panel revealed an increased level of aspartate aminotransferase (AST - 56.0 U/l), hypocalcemia (6.9 mg/dL), hypoalbuminemia (1.91 g/dL), hypobetaglobulinemia (0.51 g/dL), hypergammaglobulinemia (2.91 g/dL), and a decreased albumin/globulin (A/G) ratio (0.41). The observed hematological changes included a normocytic normochromic anemia (RBC = 2.55 x 106/mm³ , hemoglobina = 5.8 g/dL, PCV = 18%), leukocytosis (332,800/mm³) with a left shift (3,328/mm³), eosinopenia (0), monocytosis (6,656/mm³), lymphocytosis (89,856/mm³) with a pronounced presence of lymphoblasts (226,304/mm³) and thrombocytopenia (10 x 103/mm³). The blood smear evaluation revealed anisocytosis, polychromasia, hypochromia, giant platelets, smudge cells and lymphoid cells showing predominantly cytoplasmic basophilia, coarse nuclear chromatin, atypical nucleoli, asynchrony of cell maturation and degenerative cytoplasmic vacuolization, and lymphocytes with donut-shaped nuclei. The analysis of a sternal-puncture bone marrow sample revealed that the bone marrow was diffusely occupied by neoplasic lymphoblasts. Discussion: The anemia and thrombocytopenia observed in this female dog can be attributed to the growth of neoplastic cells in the bone marrow. Another finding was the presence of smudge cells, which are broken degenerated leukocytes, and the presence of these cells in large numbers has been described in humans with chronic lymphocytic leukemia or acute lymphoblastic leukemia. The observed hypoalbuminemia, hypergammaglobulinemia and reduced A/G ratio are probably due to the infl ammatory process stimulated by the leukemia. In fact, albumin is a negative acute phase protein, while an increased production of antibodies results in the increased γ-globulins. The prognosis for acute lymphoblastic leukemia is grave, as it is typically rapidly fatal. In humans, the occurrence of hypocalcemia and large number of smudge cells in cases of acute leukemia indicates a worse prognosis. In fact, this patient died one week after her clinical presentation.


Assuntos
Animais , Feminino , Cães , Doenças do Cão/sangue , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Leucemia-Linfoma Linfoblástico de Células T Precursoras/veterinária , Transtornos Mieloproliferativos/diagnóstico , Cães
16.
Acta sci. vet. (Impr.) ; 41(supl.1): Pub. 16, 2013. ilus
Artigo em Português | VETINDEX | ID: biblio-1372659

Resumo

Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy. Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffin. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34ßE12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3 antibody a qualitative method was used and neoplasia were classified as negative (-) or positive (+) and for CK34ßE12 was used semiquantitative evaluation and neoplasms were classified as negative (-), positive with focal staining (+) and positive with diffuse staining (++). The graduation for HER-2 expression was (+) for 4 (++++), were 4 (++++) was the most intensity membrane staining. For Cox-2 the system used was semiquantitative and the distribution was scored by estimating the percentage of tumor cells staining. Intensity was estimated on a scale from 0 (absent) to 12 (strong). The proliferative index was calculated by counting nuclei positive for Ki-67 (anti-MIB-1) in a total of 1000 neoplastic cells. Microscopically, was observed a proliferation of epithelial cells in papillary arrangement with delicate connective axis of support and areas of stromal invasion, and the tumor received the diagnosis of papillary adenocarcinoma of nasal sinus. Immunohistochemical analysis revealed strong staining for Cox-2 (score 12), CKAE1/AE3 positivity and CK34ßE12 (+ + +) and negative for Her-2 (+). Loss of E-cadherin expression was observed and SMA allowed the visualization of areas of basal membrane rupture. The rate of proliferation was evaluated by means of the expression of MIB-1 was high (16%). Chemotherapy was performed with carboplatin and gemcitabine. Discussion: Sinus carcinomas in general, despite their slow growth, are locally invasive and cause extensive destruction of adjacent tissues. In this case, the immunohistochemical analysis presented positive expression of CK AE1/AE3 and CK34ßE12, indicating epithelial histogenesis with basal phenotype. Strong intensity of COX-2 expression was observed, indicating a poor prognostic for the patient. The loss of expression of E-caderin, areas of invasive tumors and high rate of cell proliferation, demonstrated the aggressive character of papillary adenocarcinoma nasal sinus. Surgery with chemotherapy- associated was effective and tolerant treatment. Diagnosis was based on histopathological and immunohistochemical analysis. Correct characterization of this neoplasm associated with the appropriate therapy aided in prognosis and in the improvement of the animal's quality of life. The patient's overall survival has been evaluated since the surgical procedure up to this date and is over 18 months.


Assuntos
Animais , Masculino , Cães , Adenocarcinoma Papilar/cirurgia , Adenocarcinoma Papilar/diagnóstico , Adenocarcinoma Papilar/terapia , Neoplasias Nasais/veterinária , Doenças do Cão/diagnóstico por imagem , Cavidade Nasal/anatomia & histologia , Cães
17.
Acta sci. vet. (Impr.) ; 40(2): Pub. 1042, 2012. ilus
Artigo em Português | VETINDEX | ID: biblio-1373567

Resumo

Background: Chondrosarcoma is a malignant neoplasm originated from chondroid cells with production of cartilage matrix with lot of degrees of differentiation. It may be found in skeletal or extraskeletal tissue, more commonly in flat bones. It most often has an aggressive behavior with involvement of bone and adjacent soft tissues, which necessitates their rapid identification, surgical intervention and therapy. Chondrosarcoma is reported as uncommon neoplasm in the nasal cavity of dogs and should be distinguished by both other malignancies and for non-neoplastic diseases. Case: A 7-years-old dog, mixed breed, male, had a swelling in the nasal cavity for more than 30 days. The change was seen in the right frontal-nasal region with invasion of the oral cavity, with serous nasal discharge and also, inspiratory and expiratory noises. Even after cytology and biopsy of the tumor to chemotherapy instituted, there was not successful and the dog was euthanized. At necropsy, there was a large multilobulated mass, with firm consistency, interspersed with soft areas in the right nasal region. In the cut surface, the mass showed staining grayish-white interspersed with dark areas, which block most of the nasal cavity causing partial lysis of frontal, temporal and tubinates bones, as well as purulent secretion of the frontal sinuses (sinusitis). Microscopically, the mass consisted of mesenchymal neoplastic cells arranged in bundles or nodules without orientation, separated by fine bands of fibrous connective tissue poorly vascularized. The neoplastic cells showed round to oval nucleus, sparse chromatin and conspicuous nucleoli. Some areas were more solid and composed of cells with few cytoplasm, similar to chondroblastic cells. It was observed multifocal areas of chondroid matrix formation. Discussion: Based on pathological findings, the diagnosis of chondrosarcoma was confirmed. The histopathological findings confirmed the diagnosis of chondrosarcoma suggested by cytology. Despite being an uncommon neoplasm in dogs, chondrosarcoma should be considered as differential diagnosis of other nasal diseases that can lead macroscopic changes and similar clinical signs. The osteosarcoma is the most common bone tumor of the nasal cavity in dogs. The similarity of the macroscopic findings of chondrosarcoma and oesteosarcoma, as well as histological and immunohistochemical stain, may be a challenge to diagnosis for the clinician and pathologist. Other neoplasms such as squamous cell carcinoma, fibrosarcoma and hemangiosarcoma, as well as proliferative rhinitis caused by fungal agents, should also be considered in the differential diagnosis. These conditions can be differentiated by microscopic features and immunohistochemical stain. The fine needle aspiration biopsy is an important method, however to establish the definitive diagnosis is always required histopathologic evaluation. In primary chondrosarcoma of the nasal cavity in dogs, the survival with treatment is made from three to six months, however, often results in local severe invasion and serious clinical changes. In this case, the treatment was not effective because the degree of tumor differentiation and infiltration of the surrounding structures, as well as worsens of the clinical signs. The knowledge of the clinicopathological findings and biology of this tumor is essential for rapid diagnosis and more effective clinical interventions.


Assuntos
Animais , Masculino , Cães , Neoplasias Nasais/veterinária , Condrossarcoma/diagnóstico , Doenças do Cão/diagnóstico , Cavidade Nasal
18.
Nosso Clín. ; 19(109): 12-16, 2016. ilus
Artigo em Português | VETINDEX | ID: vti-483747

Resumo

A Osteopatia Craniomandibular é uma afecção óssea proliferativa, não neoplásica, pouco comum em cães. Acomete principalmente, os ossos da mandíbula e do crânio. Os animais mais acometidos são cães jovens, entre três a 10 meses de idade, das raças West Highland e Scottish Terrier, tendo sido relatada também em outras raças tais como Bulldog, Labrador Retriever, Boxer, Bullmastiff, Pastor de Shetland e Shar Pei. Trata-se de uma doença autolimitante e sem predileção sexual. Os principais sinais clínicos são dor em região mandibular, aumento da mandíbula, sialorreia e febre intermitente. O diagnóstico é fundamentado basicamente nos sinais clínicos e achados radiográficos. O tratamento é sintomático, controlando a dor e providenciando suporte nutricional.(AU)


The Craniomandibular Osteopathy is a proliferative bone affection, non-neoplastic and it is uncommon in dogs. Affects mainly, the jaw bones and the skull bones. The animals that are most attacked are young dogs, from the age of three months to ten months, from the breeds West Highland and Scottish Terrier although been related in other breeds like the Bulldog, Labrador Retriever, Boxer, Bullmastiff, Shetland Sheepdog and Shar Pei. It's a self-limiting disease and without sex predilection. The main clinical signals are pain in the jaw area, jaw swelling, drooling and intermittent fever. The diagnosis is based basically in the clinical signs and radiographic findings. The treatment is symptomatic, controlling the pain and providing nutritional support.(AU)


La osteopatía Craniomandibular es una enfermedad proliferativa de los huesos, no neoplásico, poco común en perros. Afecta principalmente a los huesos de la mandíbula y el cráneo. Los animales más afectados son los perros jóvenes entre tres y diez meses de edad, las razas más frecuentes son West Highland y Scottish Terrier, también ha sido reportado en otras razas como el Bulldog, Labrador, Boxer, Bullmastiff, Pastor de Shetland y Shar Pei. Esta es una enfermedad autolimitada y sin preferencia de género. Los principales signos clínicos son dolor en la región mandibular, aumento de la mandíbula, babeo y fiebre intermitente. El diagnóstico se basa principalmente en los signos clínicos y los resultados radiológicos. El tratamiento es sintomático, controlar el dolor y la prestación de apoyo nutricional.(AU)


Assuntos
Animais , Cães , Doenças Ósseas/diagnóstico , Mandíbula/patologia , Crânio/patologia , Periostite/veterinária , Crânio/lesões , Dor Facial/veterinária , Crânio
19.
Nosso clínico ; 19(109): 12-16, 2016. ilus
Artigo em Português | VETINDEX | ID: biblio-1485919

Resumo

A Osteopatia Craniomandibular é uma afecção óssea proliferativa, não neoplásica, pouco comum em cães. Acomete principalmente, os ossos da mandíbula e do crânio. Os animais mais acometidos são cães jovens, entre três a 10 meses de idade, das raças West Highland e Scottish Terrier, tendo sido relatada também em outras raças tais como Bulldog, Labrador Retriever, Boxer, Bullmastiff, Pastor de Shetland e Shar Pei. Trata-se de uma doença autolimitante e sem predileção sexual. Os principais sinais clínicos são dor em região mandibular, aumento da mandíbula, sialorreia e febre intermitente. O diagnóstico é fundamentado basicamente nos sinais clínicos e achados radiográficos. O tratamento é sintomático, controlando a dor e providenciando suporte nutricional.


The Craniomandibular Osteopathy is a proliferative bone affection, non-neoplastic and it is uncommon in dogs. Affects mainly, the jaw bones and the skull bones. The animals that are most attacked are young dogs, from the age of three months to ten months, from the breeds West Highland and Scottish Terrier although been related in other breeds like the Bulldog, Labrador Retriever, Boxer, Bullmastiff, Shetland Sheepdog and Shar Pei. It's a self-limiting disease and without sex predilection. The main clinical signals are pain in the jaw area, jaw swelling, drooling and intermittent fever. The diagnosis is based basically in the clinical signs and radiographic findings. The treatment is symptomatic, controlling the pain and providing nutritional support.


La osteopatía Craniomandibular es una enfermedad proliferativa de los huesos, no neoplásico, poco común en perros. Afecta principalmente a los huesos de la mandíbula y el cráneo. Los animales más afectados son los perros jóvenes entre tres y diez meses de edad, las razas más frecuentes son West Highland y Scottish Terrier, también ha sido reportado en otras razas como el Bulldog, Labrador, Boxer, Bullmastiff, Pastor de Shetland y Shar Pei. Esta es una enfermedad autolimitada y sin preferencia de género. Los principales signos clínicos son dolor en la región mandibular, aumento de la mandíbula, babeo y fiebre intermitente. El diagnóstico se basa principalmente en los signos clínicos y los resultados radiológicos. El tratamiento es sintomático, controlar el dolor y la prestación de apoyo nutricional.


Assuntos
Animais , Cães , Crânio/lesões , Crânio/patologia , Doenças Ósseas/diagnóstico , Mandíbula/patologia , Periostite/veterinária , Crânio , Dor Facial/veterinária
20.
Tese em Português | VETTESES | ID: vtt-201913

Resumo

As células-tronco adultas exibem relevante potencial clonogênico, fácil interação com biomateriais e capacidade de originar tecidos mesodermais e não mesodermais, sem implicações éticas e com mínimos riscos ao paciente. A busca constante por modelos biológicos que mimetizem a fisiologia e as doenças que acometem humanos, a introdução de novos modelos animais é promissora para a pesquisa médica aplicada. Dentre as qualidades que se destacam em um modelo animal, são relevantes o fácil manejo, alta prolificidade, longevidade e resistência a múltiplos procedimentos. As cutias, Ordem Rodentia, Subordem Histricognatha, são animais rústicos, com maior porte que os roedores comumente utilizados em laboratório, bastante estudadas em seus aspectos morfofisiológicos e como modelo experimental em ensaio pré-clínico. Este estudo teve como objetivo caracterizar células-tronco mesenquimais (CTM) da medula óssea e do tecido adiposo de cutias, visando à conservação destas células, para formação de biobanco, com a finalidade de preservar material genético da espécie, bem como na utilização de sua multipotencialidade em futuras terapias celulares e pesquisas na área da medicina regenerativa. Foram utilizados seis animais criados no Núcleo de Estudos e Preservação de Animais Silvestres (NEPAS) da Universidade Federal do Piauí UFPI. As cutias foram anestesiadas para coleta da medula óssea do fêmur e do tecido adiposo subcutâneo da região cervical dorsal. O isolamento das células-tronco mesenquimais da medula óssea (CTMMO) e do tecido adiposo (CTMTA) foi realizado em cultura celular a partir da fração mononuclear obtida pela separação por gradiente de densidade com Ficoll-Paque, e de fragmentos teciduais resultantes da dissociação física e química com colagenase, respectivamente. Foram realizados procedimentos de estudo in vitro com as duas linhagens celulares: Ensaio de Unidade Formadora de Colônia Fibroblastóide; curva de crescimento celular; diferenciação nas linhagens adipogênica, osteogênica e condrogênica; imunofenotipagem pela análise da expressão dos marcadores CD90, CD45 e CD14 por citometria de fluxo; e marcação com nanocristais para análise de sua manutenção por cinco passagens do cultivo. Os resultados demonstraram que as CTM das duas fontes são plástico aderentes, apresentam morfologia fibroblastóide e organizam-se em colônias mesmo quando uma baixa densidade celular inicial é semeada. Foram observadas três fases na curva de crescimento de CTMMO e CTMTA, relacionadas à fase de adaptação, proliferação exponencial e estabilidade da cinética celular. Quando induzidas à diferenciação adipogênica, osteogênica e condrogênica, demonstraram características morfológicas especializadas de cada linhagem celular, alterações semelhantes foram visualizadas em CTMMO que foram criopreservadas quando induzidas à diferenciação adipogênica e osteogênica. A análise imunofenotípica revelou que CTMMO e CTMTA não expressaram marcação de células hematopoéticas e que expressam o CD90 em diferentes níveis. Além disso, estas células incorporam nanocristais fluorescentes e podem ser identificadas através desta marcação por várias gerações celulares. Portanto, as células-tronco mesenquimais destes dois sítios apresentam elevada autorrenovação, com as CTMTA apresentando maior atividade proliferativa. A capacidade para diferenciação em três linhagens diferentes confirma a multipotencialidade destas células, que expressam marcação comum às células-tronco mesenquimais obtidas de outras fontes e animais. Ademais, a possibilidade de conservação por criopreservação e identificação por nanocristais fluorescentes favorecem sua futura aplicação em terapia celular.


Adult stem cells exhibit relevant clonogenic potential, easy interaction with biomaterials and ability to originate mesodermal and non mesodermal tissues without ethical implications and with minimal risk to the patient. Considering the constant search for biological models that mimic the physiology and diseases that affect humans, the introduction of new animal models is promising for applied medical research. Among the qualities that stand out in an animal model, they are relevant for easy handling, high prolificacy, longevity and resistance to multiple procedures. The agouti are rustic animals, Order Rodentia, Suborder Histricognatha, in addition to these characteristics, they are larger than common rodents used in the laboratory, and have been studied in their morphophysiological aspects and performed experimental preclinical tests. The objective of this study was to characterize mesenchymal stem cells (MSCs) from agoutis bone marrow and adipose tissue, aiming at the conservation of these cells to form biobank, in order to preserve genetic material of the species and the use of their multipotentiality in future cell therapies and research in the field of regenerative medicine. Six animals kept in the Núcleo de Estudos e Preservação de Animais Silvestres (NEPAS) of the Federal University of Piauí - UFPI. Agoutis were anesthetized to collect the bone marrow of the femur and the subcutaneous fatty tissue of the dorsal cervical region. The isolation of mesenchymal stem cells from bone marrow (BMSC) and adipose tissue (CTMTA) was performed in cell culture from the mononuclear fraction obtained through separation by density gradient with FicollPaque, and tissue fragments from physical and chemical dissociation with collagenase, respectively. After expansion of these two-cell types in vitro study procedures were performed: colony forming unity essay; cell growth curve; differentiation in adipogenic, osteogenic and chondrogenic lineages; analysis of the expression of CD90+, CD45- and CD14- markers by flow cytometry; and marking with nanocrystals of different passages of cultivation. The results demonstrated that MSCs from the two sources are adherent plastic, and exhibit organized fibroblastoid colonies into even when a low initial cell density. Three stages were observed in the growth curve of BMSC and CTMTA, related to the adjustment phase, exponential proliferation and stability of cell kinetics. When induced to adipogenic differentiation, osteogenic and chondrogenic demonstrated specialized morphological characteristics of each cell line, similar changes were viewed in BMSC were cryopreserved when induced with adipogenic and osteogenic differentiation. Phenotypic analysis revealed that BMSC and CTMTA not express hematopoietic cell marking and expressing CD90 at different levels. Moreover, these cells incorporate fluorescent nanocrystals and can be identified by several cell generations. Therefore, the mesenchymal stem cells of these two sites have high self renewal, with CTMTA showing higher proliferative activity. The ability to differentiate into three different lineages confirms the multipotentiality these cells, which express common marking of the mesenchymal stem cells obtained from other sources and animals. Furthermore, the possibility of preservation, cryopreservation and identification of fluorescent nanocrystals favor their use in future cell therapies.

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