Information and communication technology impact on vegetable farmers' transaction costs: evidence from China / O impacto da tecnologia da informação e comunicação nos custos de transação dos produtores de vegetais: evidências da China

This study empirically examined the impact of information and communication technology (ICT) on transaction costs in the sales stage from three dimensions of information costs, negotiation costs, and enforcing costs. The micro-sampling survey data was collected from 1338 vegetable growers in Hebei and Shandong provinces of China. We applied propensity score matching to examine the facts that adopting ICT can significantly decrease farmers' information costs through improving information accuracy and reducing searching difficulty, and reducing negotiation costs by lowering the negotiation time. However, using ICT has no significant impact on default risk and transportation difficulty thus enforcing costs of farmers have a slight difference. Therefore, a coordinated set of policies including cultivating farmers' ability to obtain information, advancing the construction of vegetable distribution channels and improving the market supervision mechanism are put forward.

Este estudo examina empiricamente o impacto da tecnologia da informação e comunicação (TIC) nos custos de transação no estágio de vendas a partir de três dimensões de custos de informação, custos de negociação e custos de execução. Os dados da pesquisa de micro-amostragem foram coletados de 1338 produtores de vegetais nas províncias de Hebei e Shandong, na China. Aplicamos a correspondência de pontuação de propensão para examinar os fatos de que a adoção de TIC pode diminuir significativamente os custos de informação dos agricultores, melhorando a precisão das informações e reduzindo a dificuldade de pesquisa, e reduzir os custos de negociação, diminuindo o tempo de negociação. No entanto, o uso de TIC não tem impacto significativo no risco de inadimplência e na dificuldade de transporte, portanto, os custos de fiscalização dos agricultores têm uma ligeira diferença. Por conseguinte, um conjunto coordenado de políticas, incluindo o cultivo da capacidade dos agricultores de obter informações, o avanço na construção de canais de distribuição de vegetais e a melhoria do mecanismo de supervisão do mercado são apresentados.

Evaluation of a YouTube channel for scientific disclosure on agribusiness / Avaliação de um canal no Youtube para divulgação científica de assuntos relacionados ao agronegócio

This research analyzed the efficiency of a channel on the YouTube platform as a strategy for scientific dissemination and rural extension. The channel was created at the end of 2018 and the last video published in March 2021, totaling 58 videos. Through the metrics made available by YouTube Analytics, the information related to the channel, the audience, the searches on the platform and the engagement of the public were analyzed. Regarding the channel's metrics, an average of 15 minutes of total viewing duration, 144 subscriptions obtained and 8 lost. The audience metrics show that the channel has WhatsApp ©, Gmail ©, university website and Google© research as its main external sources. Furthermore, there is an audience, mostly female, Brazilian, between 18 and 24 years old, with access mainly by cell phone, computer and TV. The channel searches were more relevant than those coming from the platform itself, in addition to direct or unknown origin, they had a longer average duration and hours watching, but a lower viewing rate. In the engagement, the use of the elements of the final screen was positive and more used after such confirmation, in addition, in the majority of the videos, they generated positive interactions with a majority of "I liked" instead of "I didn't like". We concluded, therefore, that in matters of scientific dissemination, the channel gained relevance when observing the growing metrics in relation to the public, while the rural extension also, however, with the participation of younger producers, requiring analyzes of how to reach the other age groups. Furthermore, it highlights the need for further research to understand better ways of engaging the public.

Esta pesquisa analisou a eficiência de um canal na plataforma YouTube como estratégia para divulgação científica e extensão rural. O canal foi criado no final de 2018 e o último vídeo publicado em março de 2021, totalizando 58 vídeos. Por meio das métricas disponibilizadas pelo YouTube Analytics, foram analisadas informações referentes ao canal, audiência, buscas na plataforma e engajamento do público. Em relação às métricas do canal, obteve-se uma média de 15 minutos de duração da visualização total. As métricas de audiência mostraram que as principais origens externas do canal são o WhatsApp©, Gmail©, sites universitários e a pesquisa do Google©. Constata-se um público majoritariamente feminino, brasileiro, entre os 18 e 24 anos de idade, com acesso principalmente pelo celular, computador e TV. As buscas do canal tiveram maior relevância em comparação àquelas oriundas da própria plataforma, além de origem direta ou desconhecida e apresentaram maior duração média e horas assistidas, porém, menor taxa de visualização. No engajamento, a utilização dos elementos da tela final foi positiva e mais utilizada após tal constatação, além disso em sua maioria os vídeos geraram interações positivas com uma maioria de "Gostei". Conclui-se, portanto, que em questões de divulgação científica o canal obteve relevância ao constatar as crescentes métricas em relação ao público. Quanto à extensão rural, foram atingidos principalmente produtores jovens, necessitando análises de como atingir as demais faixas etárias. Ademais, ressalta-se a importância de continuidade da pesquisa para entender melhores formas de engajamento do público.

Percepção do uso de animais de laboratório entre alunos do ensino médio de 11 escolas da região Centro-Leste do estado de São Paulo / The use of laboratory animals from the point of view of 11 high schools in the East-Center region of São Paulo state

O uso de animais é uma importante etapa no desenvolvimento científico e tecnológico. Porém, a maioria das pessoas desconhece que há conceitos éticos preconizados na ciência de animais de laboratório como Bem-estar Animal e o princípio dos 3Rs (do inglês: substituir, reduzir e aperfeiçoar). Desta forma, a divulgação desse conhecimento é necessária durante a formação educacional do estudante, tendo em vista os alunos como comunicadores científicos na sociedade. Assim, nosso estudo teve como objetivo avaliar o conhecimento de alunos do ensino médio de escolas particulares e públicas da região Centro-Leste do estado de São Paulo, através de formulário online sobre a temática do conhecimento básico sobre a ciência de animais de laboratório, princípios éticos, bem-estar animal, 3Rs e uso de animais em pesquisa científica. Em nosso estudo, 40 alunos responderam o questionário online. Destes 25% não souberam qual a finalidade do uso de animais em pesquisas, 90% nunca visitaram ou estiveram em um laboratório que trabalha com pesquisa biomédica, e 62,5% a maioria, nunca ouviram falar nos conceitos de Bem-estar animal e 3Rs. A maioria dos adolescentes acredita que a principal contribuição do uso de animais de laboratório se limita na elaboração de vacinas, medicamentos e soros. Sendo assim, concluímos que a inserção de conteúdos temáticos, participação dos alunos em projetos de extensão e debates sobre a ciência de animais de laboratório são extremamente necessárias na formação educacional, de modo a disseminar a compreensão sobre o assunto e evitar a propagação de falsos conhecimentos.

The use of animals is an important step in scientific and technological development in biomedical research. Animals kept in suitable conditions for scientific purposes can be considered laboratory animals. However, most people are unaware of ethical concepts used in laboratory animal science such as Animal Welfare and the principle of the 3Rs (replacement, reduction, and refinement). Dissemination of this knowledge is necessary during student education, considering the students as scientific communicators in society. The aim was to evaluate the knowledge of high school students from private and public schools in the East-Center region of São Paulo state. The analysis was performed using an online survey form about the laboratory animals science issue of basic knowledge, ethical principles, animal welfare, 3Rs, and the use of animals in scientific research. Forty students answered the online survey form. Twenty-five percent of adolescents did not know the purpose of using animals in research, 90% had never visited or been to a laboratory that works with biomedical research, and 62.5% had never heard of the concepts of animal welfare and the 3Rs. Most adolescents believe that laboratory animals' contribution is limited to the elaboration of vaccines, medicines, and serums. Therefore, we conclude that the insertion of thematic content, student participation in extension projects, and debates about the science of laboratory animals are extremely necessary for disseminating an understanding of the subject and avoiding the spread of false knowledge.

Patterns of production and sustainability of cattle ranching in the state of Pará - Brazilian Amazon / Padrões de produção e sustentabilidade da pecuária bovina no estado do Pará - Amazônia Brasileira

The aim of this study was to analyze the production patterns present in rural properties producing cattle in the micro-regions that make up the state of Pará. Exploratory Factor Analysis (EFA) was applied to identify the patterns, and these data are used to evaluate correlation between the heterogeneity of rural properties and the environmental impact on the identified patterns. The theoretical contribution is based on discussions on global impacts of food production and environmental sustainability and the impacts of livestock production systems in Brazil and the Amazon. Survey data were taken from the 2017 Agricultural Census, available for the 144 municipalities in the state, and pooled into 22 micro-regions. Three patterns of rural properties were identified: the first related to conservation management practices and called transition management; the second highlights aspects associated with information technology and communication (ICT) and productivity called technical productive efficiency; the third indicates the importance of social organization and access to information called social participation. With these patterns, it was possible to develop the Traditional Performance Indicator (TPI), in which the micro-regions of São Félix do Xingu, Itaituba and Conceição do Araguaia were those with the highest values of this indicator, water protection practices are present in the properties, however, in precarious conditions, and conservation practices are rarely used. The correlation between heterogeneity, measured by the size of pasture area in each microregion, and the TPI is positive, strong and significant. In this transition context, public policies are essential to provide access to infrastructure, credit and good animal health and biotechnology practices.(AU)

O objetivo deste estudo foi analisar os padrões de produção presentes nos estabelecimentos rurais produtores de gado bovino nas microrregiões que formam o estado do Pará. Foi aplicada a técnica estatística de Análise Fatorial Exploratória (AFE) para identificar os padrões, e com esses dados avaliar a correlação entre a heterogeneidade das propriedades rurais produtoras e o impacto ambiental nos padrões identificados. O aporte teórico está ancorado nas discussões sobre os impactos globais da produção de alimentos e a sustentabilidade ambiental e os impactos da pecuária no Brasil e na Amazônia. Os dados da pesquisa foram extraídos do Censo Agropecuário de 2017, disponíveis para os 144 municípios do estado, e agregados em 22 microrregiões. Foram identificados três padrões de estabelecimentos rurais: o primeiro relacionado as práticas de gestão conservacionistas e denominado de gestão em transição; o segundo destaca aspectos associados a tecnologia de informática e comunicação (TIC) e a produtividade denominado de eficiência técnica produtiva; o terceiro indica a importância da organização social e o acesso a informação denominado de participação social. Com os resultados desses padrões foi possível gerar o Indicador de Desempenho Tradicional (IDT), em que as microrregiões de São Félix do Xingu, Itaituba e Conceição do Araguaia foram aquelas com maior nível desse indicador, as práticas de proteção das águas se fazem presente nas propriedades, porém, em condições precárias, e as práticas conservacionistas são pouco empregadas. A correlação entre a heterogeneidade, medida pelo tamanho da área de pasto em cada microrregião, e o IDT é positiva, forte e significativa. Nesse contexto de transição, as políticas públicas são essenciais para proporcionar acesso a infraestrutura, crédito e boas práticas de saúde animal e biotecnologia.(AU)

Information technology by mobile communication for the notification of canine visceral leishmaniasis / Tecnologia da informação por comunicação móvel para notificação de leishmaniose visceral canina

Visceral leishmaniasis is the most severe form of a human and animal disease complex entitled leishmaniasis, which is endemic to 70 countries. It is imperative to develop and offer technologies capable of increasing the resolution ability of control programs of this zoonosis. In the search for technological innovations in health, especially in environmental surveillance, the objective is to develop a mobile application (App) for smartphones in order to facilitate and systematize the notification of positive cases of canine visceral leishmaniasis (CVL) by veterinarians working in clinics for assisting the municipal health surveillance in the management of this zoonosis. Thus, we developed an App, C7 LVC - Canine Visceral Leishmaniasis Notification System, with formatting based on the CR Campeiro 7® software. The technology created enables the filling of important gaps in information systems, facilitating the transmission of data and the use of this data by public management bodies to take CVL prevention and control actions.(AU)

A leishmaniose visceral é a forma mais grave de um complexo de doenças humanas e animais, denominado leishmaniose, endêmica em 70 países. É imprescindível desenvolver e oferecer tecnologias capazes de aumentar a capacidade de resolução dos programas de controle desta zoonose. Na busca por inovações tecnológicas em saúde, principalmente na vigilância ambiental, o objetivo é desenvolver um aplicativo móvel (App) para smartphones a fim de facilitar e sistematizar a notificação de casos positivos de leishmaniose visceral canina (LVC) por médicos veterinários que atuam em clínicas auxiliando a vigilância sanitária municipal, na gestão desta zoonose. Para tanto, foi desenvolvido um App, C7 LVC - Sistema de Notificação da Leishmaniose Visceral Canina, com formatação baseada no software CR Campeiro 7®. A tecnologia criada possibilita o preenchimento de lacunas importantes nos sistemas de informação, facilitando a transmissão de dados e a utilização desses dados pelos órgãos da gestão pública para a tomada de ações de prevenção e controle da LVC.(AU)

Information technology by mobile communication for the notification of canine visceral leishmaniasis / Tecnologia da informação por comunicação móvel para notificação de leishmaniose visceral canina

Visceral leishmaniasis is the most severe form of a human and animal disease complex entitled leishmaniasis, which is endemic to 70 countries. It is imperative to develop and offer technologies capable of increasing the resolution ability of control programs of this zoonosis. In the search for technological innovations in health, especially in environmental surveillance, the objective is to develop a mobile application (App) for smartphones in order to facilitate and systematize the notification of positive cases of canine visceral leishmaniasis (CVL) by veterinarians working in clinics for assisting the municipal health surveillance in the management of this zoonosis. Thus, we developed an App, C7 LVC - Canine Visceral Leishmaniasis Notification System, with formatting based on the CR Campeiro 7® software. The technology created enables the filling of important gaps in information systems, facilitating the transmission of data and the use of this data by public management bodies to take CVL prevention and control actions.(AU)

A leishmaniose visceral é a forma mais grave de um complexo de doenças humanas e animais, denominado leishmaniose, endêmica em 70 países. É imprescindível desenvolver e oferecer tecnologias capazes de aumentar a capacidade de resolução dos programas de controle desta zoonose. Na busca por inovações tecnológicas em saúde, principalmente na vigilância ambiental, o objetivo é desenvolver um aplicativo móvel (App) para smartphones a fim de facilitar e sistematizar a notificação de casos positivos de leishmaniose visceral canina (LVC) por médicos veterinários que atuam em clínicas auxiliando a vigilância sanitária municipal, na gestão desta zoonose. Para tanto, foi desenvolvido um App, C7 LVC - Sistema de Notificação da Leishmaniose Visceral Canina, com formatação baseada no software CR Campeiro 7®. A tecnologia criada possibilita o preenchimento de lacunas importantes nos sistemas de informação, facilitando a transmissão de dados e a utilização desses dados pelos órgãos da gestão pública para a tomada de ações de prevenção e controle da LVC.(AU)

Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus

Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making their skin (and cutaneous secretion) rich in bioactive molecules. Among the tree frogs, the secretion is composed mainly of peptides; but alkaloids, proteins and steroids can also be found depending on the species. The most known class of biologically active molecules is the antimicrobial peptides (AMPs) that act against bacteria, fungi and protozoans. Although these molecules are well-studied among the hylids, AMPs ontogeny remains unknown. Therefore, we performed peptidomic and proteomic analyses of Pithecopus nordestinus (formerly Phyllomedusa nordestina) in order to evaluate the peptide content in post-metamorphosed juveniles and adult individuals. Methods: Cutaneous secretion of both life stages of individuals was obtained and analyzed by LC-MS/MS after reduction and alkylation of disulfide bonds or reduction, alkylation and hydrolysis by trypsin. Results: Differences in the TIC profile of juveniles and adults in both treatments were observed. Moreover, the proteomic data revealed known proteins and peptides, with slight differences in the composition, according to the life stage and the treatment. AMPs were identified, and bradykinin-potentiating peptides were observed in trypsin-treated samples, which suggests a protein source of such peptide (cryptide). Conclusion: In general, skin secretion contents were similar between juveniles and adults, varying in quantity, indicating that the different stages of life are reflected in the number of molecules and not on their diversity.(AU)

Quantity - but not diversity - of secreted peptides and proteins increases with age in the tree frog Pithecopus nordestinus

Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making their skin (and cutaneous secretion) rich in bioactive molecules. Among the tree frogs, the secretion is composed mainly of peptides; but alkaloids, proteins and steroids can also be found depending on the species. The most known class of biologically active molecules is the antimicrobial peptides (AMPs) that act against bacteria, fungi and protozoans. Although these molecules are well-studied among the hylids, AMPs ontogeny remains unknown. Therefore, we performed peptidomic and proteomic analyses of Pithecopus nordestinus (formerly Phyllomedusa nordestina) in order to evaluate the peptide content in post-metamorphosed juveniles and adult individuals. Methods: Cutaneous secretion of both life stages of individuals was obtained and analyzed by LC-MS/MS after reduction and alkylation of disulfide bonds or reduction, alkylation and hydrolysis by trypsin. Results: Differences in the TIC profile of juveniles and adults in both treatments were observed. Moreover, the proteomic data revealed known proteins and peptides, with slight differences in the composition, according to the life stage and the treatment. AMPs were identified, and bradykinin-potentiating peptides were observed in trypsin-treated samples, which suggests a protein source of such peptide (cryptide). Conclusion: In general, skin secretion contents were similar between juveniles and adults, varying in quantity, indicating that the different stages of life are reflected in the number of molecules and not on their diversity.(AU)

Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis

Background Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia decorata ventralis, was investigated through a comprehensive analysis using LC-MS. Two peptides were synthesized, and their neuroprotective properties were evaluated. Methods A reverse-phase HPLC connected to ESI-MS was used for LC-MS analyses. Online mass fingerprinting was performed from TIC, and data-dependent tandem mass spectrometry gave the MS/MS spectra. The sequences of two major peptide components were determined by MALDI-TOF/TOF MS analysis, confirmed by solid phase synthesis. Using the synthetic peptides, biological activities were assessed. Cell integrity tests and neuroprotection analyzes using H2O2 as an oxidative stress inducer were performed for both peptides. Results Online mass fingerprinting revealed that the venom contains 123 components, and the MS/MS analysis resulted in 33 full sequences of peptide components. The two main peptides, α-scoliidine (DYVTVKGFSPLR) and β-scoliidine (DYVTVKGFSPLRKA), present homology with the bradykinin C-terminal. Despite this, both peptides did not behave as substrates or inhibitors of ACE, indicating that they do not interact with this metallopeptidase. In further studies, β-scoliidine, but not α -scoliidine, showed protective effects against oxidative stress-induced neurotoxicity in PC12 cells through integrity and metabolism cell assays. Interestingly, β-scoliidine has the extension of the KA dipeptide at the C-terminal in comparison with α-scoliidine. Conclusion Comprehensive LC-MS and MS/MS analyses from the Scolia decorata ventralis venom displayed the component profile of this venom. β-scoliidine showed an effective cytoprotective effect, probably due to the observed increase in the number of cells. This is the first report of solitary wasp venom peptides showing neuroprotective activity.(AU)

Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis

Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia decorata ventralis, was investigated through a comprehensive analysis using LC-MS. Two peptides were synthesized, and their neuroprotective properties were evaluated. Methods A reverse-phase HPLC connected to ESI-MS was used for LC-MS analyses. Online mass fingerprinting was performed from TIC, and data-dependent tandem mass spectrometry gave the MS/MS spectra. The sequences of two major peptide components were determined by MALDI-TOF/TOF MS analysis, confirmed by solid phase synthesis. Using the synthetic peptides, biological activities were assessed. Cell integrity tests and neuroprotection analyzes using H2O2 as an oxidative stress inducer were performed for both peptides. Results Online mass fingerprinting revealed that the venom contains 123 components, and the MS/MS analysis resulted in 33 full sequences of peptide components. The two main peptides, α-scoliidine (DYVTVKGFSPLR) and β-scoliidine (DYVTVKGFSPLRKA), present homology with the bradykinin C-terminal. Despite this, both peptides did not behave as substrates or inhibitors of ACE, indicating that they do not interact with this metallopeptidase. In further studies, β-scoliidine, but not α -scoliidine, showed protective effects against oxidative stress-induced neurotoxicity in PC12 cells through integrity and metabolism cell assays. Interestingly, β-scoliidine has the extension of the KA dipeptide at the C-terminal in comparison with α-scoliidine. Conclusion Comprehensive LC-MS and MS/MS analyses from the Scolia decorata ventralis venom displayed the component profile of this venom. β-scoliidine showed an effective cytoprotective effect, probably due to the observed increase in the number of cells. This is the first report of solitary wasp venom peptides showing neuroprotective activity.(AU)

Intention to adopt Electronic Animal Movement Permit (e-GTA) systems in Rio Grande do Sul, Brazil / Intenção de adoção de Guia de Trânsito Animal Eletrônica (e-GTA) no Rio Grande do Sul, Brasil

Control of livestock and their movement has long been recognized as a crucial for the prevention and control of diseases. In Brazil, the control of livestock movement established in 1934. Since 1995 is regulated based on the Animal Movement Permit (GTA). Since then, the process has been improved and updated on its legal framework and strategies, including the use of emerging technological alternatives, which made possible the launching of the electronic Animal Movement Permit (e-GTA) in 2011. From a broader perspective, the e-GTA is inserted in a global context of the development of Information and Communication Technology (ICT) which since the early 1980s enabled governments worldwide to drive policies for the development of electronic government systems (e-government). After that, at a global level, there was an expansion and improvement of e-government services; however, discrepancies among countries persisted. Nonetheless, the levels of adoption of e-government by citizens have been lower than those expected by government authorities, which has attracted the attention of researchers in the area. In this context, studies of this nature that used theoretical antecedents related to the adoption of ICT have exposed peculiarities of the process of adoption of e-government, demanding research efforts directed to the structuring of specific models for this area. In Brazil, studies of this nature are still at an early stage, although government actions aimed at e-government date back to the 1990s. The objective of this article was to investigate, in an exploratory way, influence factors on the adoption intention of e-government related to animal health, having the e-GTA as the object of research. The conceptual framework was defined based upon the e-Government Adoption Model (GAM) For this objective, an online questionnaire oriented to intention to adopt e-GTA was applied to equine owners of Rio Grande do Sul not users of this system...(AU)

O controle dos estoques e de movimentações de animais pecuários há muito tempo é reconhecido como um fator crucial para a prevenção e resposta para a introdução e disseminação de doenças animais ou zoonóticas. No Brasil, o controle de movimentação animal foi inicialmente estabelecido em 1934 e, desde 1995, é regulamentado com base na Guia de Trânsito Animal (GTA). Desde então, o processo foi aprimorado e atualizado em seu arcabouço legal e estratégias, inclusive no uso de alternativas tecnológicas emergentes, que possibilitaram o lançamento em 2011 da Guia de Trânsito Animal Eletrônica (e-GTA). Considerado desde uma perspectiva mais abrangente, o e-GTA está inserido em um contexto global de desenvolvimento da Tecnologia da Informação e Comunicação (TIC), que desde o início dos anos 80 permite que governos em todo o mundo conduzam políticas para o desenvolvimento de sistemas de governo eletrônico (governo eletrônico). Posteriormente, a disponibilidade do governo eletrônico tem estado em expansão de qualidade e de amplitude dos serviços oferecidos, embora este processo seja permeado por desigualdades entre os países. No entanto, os níveis de adoção do governo eletrônico pelos cidadãos têm sido inferiores aos esperados pelas autoridades governamentais, o que tem atraído a atenção dos pesquisadores da área. Nesse contexto, estudos dessa natureza, que utilizaram antecedentes teóricos relacionados à adoção das TIC expuseram idiossincrasias do processo de adoção do governo eletrônico, exigindo que esforços de pesquisa sejam direcionados à estruturação de modelos específicos para essa área. No Brasil, estudos dessa natureza ainda estão em estágio inicial, embora as ações governamentais direcionadas ao governo eletrônico datem dos anos 90. Dessa forma, o objetivo deste artigo foi investigar, de forma exploratória, fatores de influência na intenção de adoção do governo eletrônico relacionado à saúde animal, tendo o e-GTA como objeto de pesquisa...(AU)

Intention to adopt Electronic Animal Movement Permit (e-GTA) systems in Rio Grande do Sul, Brazil / Intenção de adoção de Guia de Trânsito Animal Eletrônica (e-GTA) no Rio Grande do Sul, Brasil

Control of livestock and their movement has long been recognized as a crucial for the prevention and control of diseases. In Brazil, the control of livestock movement established in 1934. Since 1995 is regulated based on the Animal Movement Permit (GTA). Since then, the process has been improved and updated on its legal framework and strategies, including the use of emerging technological alternatives, which made possible the launching of the electronic Animal Movement Permit (e-GTA) in 2011. From a broader perspective, the e-GTA is inserted in a global context of the development of Information and Communication Technology (ICT) which since the early 1980s enabled governments worldwide to drive policies for the development of electronic government systems (e-government). After that, at a global level, there was an expansion and improvement of e-government services; however, discrepancies among countries persisted. Nonetheless, the levels of adoption of e-government by citizens have been lower than those expected by government authorities, which has attracted the attention of researchers in the area. In this context, studies of this nature that used theoretical antecedents related to the adoption of ICT have exposed peculiarities of the process of adoption of e-government, demanding research efforts directed to the structuring of specific models for this area. In Brazil, studies of this nature are still at an early stage, although government actions aimed at e-government date back to the 1990s. The objective of this article was to investigate, in an exploratory way, influence factors on the adoption intention of e-government related to animal health, having the e-GTA as the object of research. The conceptual framework was defined based upon the e-Government Adoption Model (GAM) For this objective, an online questionnaire oriented to intention to adopt e-GTA was applied to equine owners of Rio Grande do Sul not users of this system. Results indicated that respondents positively evaluated the quality of their computers and cellphones, as well as their efficacy to operate them, while quality of the internet connection was considered regular. In this context, the availability of computer and cellphone resources and computer self-efficacy did not influence the intention to adopt e-GTA. Moreover, aspects related to compatibility of e-GTA with users lifestyle have a positive association with the intention to adopt e-GTA. Also the study indicated that public information campaigns could contribute to the improvement of adoption of the e-GTA. Additionally, the authors suggested that further investigations considering other livestock sectors and variables, as well as the continuity of use after the adoption, could contribute to the expansion of the knowledge on this field.(AU)

O controle dos estoques e de movimentações de animais pecuários há muito tempo é reconhecido como um fator crucial para a prevenção e resposta para a introdução e disseminação de doenças animais ou zoonóticas. No Brasil, o controle de movimentação animal foi inicialmente estabelecido em 1934 e, desde 1995, é regulamentado com base na Guia de Trânsito Animal (GTA). Desde então, o processo foi aprimorado e atualizado em seu arcabouço legal e estratégias, inclusive no uso de alternativas tecnológicas emergentes, que possibilitaram o lançamento em 2011 da Guia de Trânsito Animal Eletrônica (e-GTA). Considerado desde uma perspectiva mais abrangente, o e-GTA está inserido em um contexto global de desenvolvimento da Tecnologia da Informação e Comunicação (TIC), que desde o início dos anos 80 permite que governos em todo o mundo conduzam políticas para o desenvolvimento de sistemas de governo eletrônico (governo eletrônico). Posteriormente, a disponibilidade do governo eletrônico tem estado em expansão de qualidade e de amplitude dos serviços oferecidos, embora este processo seja permeado por desigualdades entre os países. No entanto, os níveis de adoção do governo eletrônico pelos cidadãos têm sido inferiores aos esperados pelas autoridades governamentais, o que tem atraído a atenção dos pesquisadores da área. Nesse contexto, estudos dessa natureza, que utilizaram antecedentes teóricos relacionados à adoção das TIC expuseram idiossincrasias do processo de adoção do governo eletrônico, exigindo que esforços de pesquisa sejam direcionados à estruturação de modelos específicos para essa área. No Brasil, estudos dessa natureza ainda estão em estágio inicial, embora as ações governamentais direcionadas ao governo eletrônico datem dos anos 90. Dessa forma, o objetivo deste artigo foi investigar, de forma exploratória, fatores de influência na intenção de adoção do governo eletrônico relacionado à saúde animal, tendo o e-GTA como objeto de pesquisa. A estrutura conceitual foi definida com base no Modelo de Adoção de Governo Eletrônico (GAM). Para esse objetivo, um questionário online dirigido à intenção de adoção do e-GTA foi aplicado a proprietários de equinos do Rio Grande do Sul que não eram usuários deste sistema. Os resultados indicaram que os entrevistados avaliaram positivamente a qualidade de seus computadores e telefones celulares, bem como sua eficácia em operá-los, enquanto a qualidade da conexão de internet foi considerada regular. Nesse contexto, a disponibilidade de recursos informáticos e de celulares, bem como a capacidade de operação dos mesmos não influenciaram a intenção de adoção do e-GTA. Além disso, aspectos relacionados à compatibilidade do e-GTA com o estilo de vida do usuário têm uma associação positiva com a intenção de adoção do e-GTA. Além disso, o estudo indicou que as campanhas de informação pública poderiam contribuir para o incremento na adoção efetiva do e-GTA. Finalmente, o estudo identificou oportunidades para o desenvolvimento de investigações adicionais, considerando distintas variáveis, setores produtivos e sistemas de governo eletrônico dirigidos à saúde animal, o que poderia contribuir para a expansão do conhecimento nesta área.(AU)

Neurite aguda do nervo trigêmeo em Pastor Alemão / Acute trigeminal nerve neuritis in German Shepherd

Background: Trigeminal nerve is composed by ophthalmic, maxillary and mandibular portion, presenting sensory and motor functions. Its most common conditions include vascular, neoplastic, infectious and inflammatory causes. Neuritis is an inflammation caused by a primary nerve injury that can progress to demyelination and even degeneration of nerve fibers. The present report aims to describe an unusual case of a female dog, German Shepherd breed, with acute manifestation of trigeminal nerve neuritis whose etiology may be associated with erlichiosis, since infection with Ehrlichia spp. through serological test was verified. Case: A 3-year-old female German Shepherd, weighing 26.8 kg and not neutered, was attended at Veterinary Hospital Dr. Halim Atique - UNIRP, São José do Rio Preto, SP, Brazil presenting apathy, sialorrhea and polydipsia for seven days. The tutor reported an episode of foamy and yellowish vomit three days ago and ixodidiosis last week. Physical examination revealed flaccid open-mouthed posture, with mild bilateral masseter and moderate temporal muscle atrophy. Water was offered to the animal and it was observed that it could not properly seize, confirming a false polydipsia. The neurological examination revealed a slight decrease in head sensitivity, difficulty in chewing and seizure of food. It was not observed alterations in the other pairs of cranial nerves or other neurological parameters (postural reactions and spinal reflexes), and the diagnose of bilateral dysfunction of the trigeminal nerve was based on the affected neuroanatomic region. On neurological examination, other lesions of the nervous system were ruled out, suggesting an isolated manifestation of the trigeminal nerve. CBC revealed anemia, intense thrombocytopenia and leukocytosis by neutrophilia. Radiographic examination ruled out the possibility of trauma due to the absence of mandible fracture and also temporomandibular joint alterations. Masseter and temporal muscle biopsy were performed, and myositis

Neurite aguda do nervo trigêmeo em Pastor Alemão / Acute trigeminal nerve neuritis in German Shepherd

Background: Trigeminal nerve is composed by ophthalmic, maxillary and mandibular portion, presenting sensory and motor functions. Its most common conditions include vascular, neoplastic, infectious and inflammatory causes. Neuritis is an inflammation caused by a primary nerve injury that can progress to demyelination and even degeneration of nerve fibers. The present report aims to describe an unusual case of a female dog, German Shepherd breed, with acute manifestation of trigeminal nerve neuritis whose etiology may be associated with erlichiosis, since infection with Ehrlichia spp. through serological test was verified. Case: A 3-year-old female German Shepherd, weighing 26.8 kg and not neutered, was attended at Veterinary Hospital Dr. Halim Atique - UNIRP, São José do Rio Preto, SP, Brazil presenting apathy, sialorrhea and polydipsia for seven days. The tutor reported an episode of foamy and yellowish vomit three days ago and ixodidiosis last week. Physical examination revealed flaccid open-mouthed posture, with mild bilateral masseter and moderate temporal muscle atrophy. Water was offered to the animal and it was observed that it could not properly seize, confirming a false polydipsia. The neurological examination revealed a slight decrease in head sensitivity, difficulty in chewing and seizure of food. It was not observed alterations in the other pairs of cranial nerves or other neurological parameters (postural reactions and spinal reflexes), and the diagnose of bilateral dysfunction of the trigeminal nerve was based on the affected neuroanatomic region. On neurological examination, other lesions of the nervous system were ruled out, suggesting an isolated manifestation of the trigeminal nerve. CBC revealed anemia, intense thrombocytopenia and leukocytosis by neutrophilia. Radiographic examination ruled out the possibility of trauma due to the absence of mandible fracture and also temporomandibular joint alterations. Masseter and temporal muscle biopsy were performed, and myositis…(AU)

Farmers use of mobile phone for accessing agricultural information in Pakistan / Papel do celular para entrega de informações agrícolas em um distrito carente da província de Punjab, Paquistão

Agriculture is considered as the main driving force in Pakistans economy employing 45% of the countrys labor force and generating 20% of national GDP Agriculture sector of Pakistan is facing numerous challenges including non-adoption of agricultural technology at the farm level, due to farmers lack of access to the latest information. In this context, the current study focusses on the use of the mobile phone in accessing agricultural information among the farmers of the of district Muzaffargarh,Punjab, Pakistan. A multistage sampling technique was used to collect data from the two tehsils (cities/sub-districts) of Muzaffargarh formulating a total sample of 180 farmers. SPSS computer-based Software was used for analyzing the data. Results revealed that 91.2% of the farmers indicated mobile phone ownership. It was further reported that 87.20 % of the farmers used private sectors advisory staff to obtain agriculture information. Market information was ranked as the highest accessed information among the rest of farm-related use. Easy access to updated information and connectivity with stakeholders were reported as the highest perceived benefits of mobile phone use (4.63 & 4.72 mean). Findings reported that farmers limited aptitude of Mobile phone usage and lack of awareness of information sources as major constraints in farm-related use of the Mobile phone. On the basis of findings, the current study provided recommendations and policy implications for utilizing the true potential of these ICT-enabled solutions in agriculture.(AU)

A agricultura é considerada como a principal força motriz na economia do Paquistão, empregando 45% da força de trabalho do país e gerando 20% do PIB nacional. O setor agrícola dos paquistaneses enfrenta inúmeros desafios, incluindo a não-adaptação da tecnologia agrícola entre os agricultores, devido a falta de acesso dos agricultores às informações agrícolas mais recentes. Neste contexto, o estudo atual enfoca o papel do telefone celular na disseminação de informações agrícolas entre os agricultores do distrito Muzaffargarhof Punjab, Paquistão. Uma técnica de amostragem em múltiplos estágios foi usada para coletar dados dos dois tehsils (cidades / sub-distritos) de Muzaffargarh, formulando uma amostra total de 180 agricultores. O software baseado em computador SPSS foi usado para analisar os dados. Os resultados mostraram que 91,2% dos agricultores tinham propriedade de telefones celulares. Verificou-se ainda que 87,20% dos agricultores contatam o pessoal consultivo do setor privado para obter informações sobre a agricultura. As informações de mercado foram classificadas como as mais acessadas entre os demais usos relacionados à fazenda (média de 41,45). O acesso fácil a informações atualizadas e a conectividade com as partes interessadas são relatados como os maiores benefícios percebidos do uso de telefones celulares entre os agricultores com valores médios de 4,63. e 4,72, respectivamente. Os resultados relataram que a capacidade limitada dos agricultores de usar o telefone celular e a falta de conhecimento das fontes de informação como principais restrições ao uso do telefone celular relacionado à fazenda. Com base nos resultados, recomenda-se a realização de sessões de treinamento com as comunidades agrícolas para criar conscientização e educá-las sobre o uso do telefone celular.(AU)

Storage of soybean seeds treated with chemicals / Armazenamento de sementes de soja tratadas com produtos químicos

To treat seed lots during the beneficiation process industrial seed treatment (TIS) has been used on seedlings. However, the actual TIS influence on the physiological quality of soybeans throughout storage is not yet known. The objective of this work was to evaluate the effects of TIS on the physiological quality of seeds of soybean cultivars during storage. Three soybean cultivars (M7110 IPRO, RR-8473RSF, M7739 IPRO) were subjected to four chemical treatment combinations (TIC-treated fungicide / insecticide): T1 - control; T2 - fungicide (Derosal Plus® - 200mL); T3 - insecticide (Cruiser ® - 500mL); T4 - a mixture of fungicide and insecticide products metalaxyl + thiabendazole + fludioxonil (MaximAdvanced - 200 mL) and thiamethoxam insecticide (Cruiser 350 FS - 500 mL). The samples were stored for a total of six months, with evaluation before storage and monthly throughout the storage period (0, 30, 60, 90, 120, 150 and 180 days). The seeds were stored in a cold chamber regulated at 12 ± 2 ºC and a relative humidity of 45± 2%. In addition to determining the water content, the following germination and vigor tests were performed: first count, seedling length, seedling dry mass and accelerated aging. A completely randomized design was used, in a 3 x 4 x 7 factorial scheme, with four replications. The data were initiallysubjected to analysis of variance, and later discriminated by the Tukey test (p <0.05) (cultivars and chemical products) and regression (storage period). The work concludes that the use of insecticide favored the length of seedlings in cultivar RR-8473 RSF. Chemicals affected the quality and vigor of seeds in all studied cultivars. Soybean seeds can be stored with chemical treatment for commercial purposes for up to 60 days.

Com a finalidade de tratar lotes de sementes durante o processo de beneficiamento as sementeiras passam a utilizar o tratamento industrial de sementes (TIS). Todavia, não se sabe ainda qual a real influência TIS sobre a qualidade fisiológica de sementes de soja ao longo do armazenamento. Este trabalho teve por objetivo avaliar os efeitos do tratamento com produtos químicos oriundos do TIS sobre a qualidade fisiológica de sementes de cultivares de soja durante o armazenamento. Três cultivares de soja (M7110 IPRO; RR- 8473RSF, M7739 IPRO) foram submetidas a quatro combinações de tratamentos com defensivos químicos (fungicida/inseticida) aplicados via TIS: {T1- testemunha; T2- fungicida (Derosal Plus® - 200mL); T3- inseticida (Cruiser ® - 500mL); T4- mistura dos produtos fungicida e inseticida}, e armazenadas por seis meses, com avaliações mensais (0; 30; 60; 90; 120; 150 e 180 dias). As sementes foram armazenadas em câmara fria regulada a 12 ± 2 ºC e umidade relativa de ±45%. Além da determinação do teor de água, foram realizadas teste de germinação e vigor : primeira contagem, comprimento de plântula, massa seca de plântula e envelhecimento acelerado. Foi empregado o delineamento inteiramente casualizado, em esquema fatorial 3 x 4 x 7, com quatro repetições.Os dados obtidos foram submetidos à análise de variância inicialmente, e posteriormente discriminados pelo teste de Tukey (p<0,05) (cultivares e produtos químicos) e regressão (período de armazenamento). Conclui-se com o trabalho que o uso de inseticida favoreceu o comprimento de plântulas na cultivar RR-8473 RSF. Produtos químicos prejudicaram a qualidade e o vigor de sementes em todas as cultivares estudadas. As sementes de soja podem ser mantidas armazenadas com tratamento químico para fins comerciais até os 60 dias.

Storage of soybean seeds treated with chemicals / Armazenamento de sementes de soja tratadas com produtos químicos

To treat seed lots during the beneficiation process industrial seed treatment (TIS) has been used on seedlings. However, the actual TIS influence on the physiological quality of soybeans throughout storage is not yet known. The objective of this work was to evaluate the effects of TIS on the physiological quality of seeds of soybean cultivars during storage. Three soybean cultivars (M7110 IPRO, RR-8473RSF, M7739 IPRO) were subjected to four chemical treatment combinations (TIC-treated fungicide / insecticide): T1 - control; T2 - fungicide (Derosal Plus® - 200mL); T3 - insecticide (Cruiser ® - 500mL); T4 - a mixture of fungicide and insecticide products metalaxyl + thiabendazole + fludioxonil (MaximAdvanced - 200 mL) and thiamethoxam insecticide (Cruiser 350 FS - 500 mL). The samples were stored for a total of six months, with evaluation before storage and monthly throughout the storage period (0, 30, 60, 90, 120, 150 and 180 days). The seeds were stored in a cold chamber regulated at 12 ± 2 ºC and a relative humidity of 45± 2%. In addition to determining the water content, the following germination and vigor tests were performed: first count, seedling length, seedling dry mass and accelerated aging. A completely randomized design was used, in a 3 x 4 x 7 factorial scheme, with four replications. The data were initiallysubjected to analysis of variance, and later discriminated by the Tukey test (p <0.05) (cultivars and chemical products) and regression (storage period). The work concludes that the use of insecticide favored the length of seedlings in cultivar RR-8473 RSF. Chemicals affected the quality and vigor of seeds in all studied cultivars. Soybean seeds can be stored with chemical treatment for commercial purposes for up to 60 days.(AU)

Com a finalidade de tratar lotes de sementes durante o processo de beneficiamento as sementeiras passam a utilizar o tratamento industrial de sementes (TIS). Todavia, não se sabe ainda qual a real influência TIS sobre a qualidade fisiológica de sementes de soja ao longo do armazenamento. Este trabalho teve por objetivo avaliar os efeitos do tratamento com produtos químicos oriundos do TIS sobre a qualidade fisiológica de sementes de cultivares de soja durante o armazenamento. Três cultivares de soja (M7110 IPRO; RR- 8473RSF, M7739 IPRO) foram submetidas a quatro combinações de tratamentos com defensivos químicos (fungicida/inseticida) aplicados via TIS: {T1- testemunha; T2- fungicida (Derosal Plus® - 200mL); T3- inseticida (Cruiser ® - 500mL); T4- mistura dos produtos fungicida e inseticida}, e armazenadas por seis meses, com avaliações mensais (0; 30; 60; 90; 120; 150 e 180 dias). As sementes foram armazenadas em câmara fria regulada a 12 ± 2 ºC e umidade relativa de ±45%. Além da determinação do teor de água, foram realizadas teste de germinação e vigor : primeira contagem, comprimento de plântula, massa seca de plântula e envelhecimento acelerado. Foi empregado o delineamento inteiramente casualizado, em esquema fatorial 3 x 4 x 7, com quatro repetições.Os dados obtidos foram submetidos à análise de variância inicialmente, e posteriormente discriminados pelo teste de Tukey (p<0,05) (cultivares e produtos químicos) e regressão (período de armazenamento). Conclui-se com o trabalho que o uso de inseticida favoreceu o comprimento de plântulas na cultivar RR-8473 RSF. Produtos químicos prejudicaram a qualidade e o vigor de sementes em todas as cultivares estudadas. As sementes de soja podem ser mantidas armazenadas com tratamento químico para fins comerciais até os 60 dias.(AU)

Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans

Abstract Background: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS. Methods: A reverse-phase HPLC connected to ESI-OrbiTrap MS was used for LC-MS. On-line mass fingerprinting was made from TIC, and data-dependent tandem mass spectrometry gave MSMS spectra. A major peptide component was isolated by reverse-phase HPLC by conventional way, and its sequence was determined by Edman degradation, which was finally corroborated by solid phase synthesis. Using the synthetic specimen, biological activities (antimicrobial activity, mast cell devaluation, hemolysis, leishmanicidal activity) and pore formation in artificial lipid bilayer were evaluated. Results: On-line mass fingerprinting revealed that the crude venom contained 124 components. MS/MS analysis gave 75 full sequences of the peptide components. Most of these are related to the major and novel peptide, xylopin. Its sequence, GFVALLKKLPLILKHLH-NH2, has characteristic features of linear cationic -helical peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, it can be predicted to adopt an amphipathic -helix secondary structure. In biological evaluation, xylopin exhibited broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. Additionally, the peptide was able to incorporate pores in artificial lipid bilayers of azolectin, confirming the mechanism of the cytolytic activity by pore formation in biological membranes. Conclusions: LC-ESI-MS and MS/MS analysis of the crude venom extract from a solitary bee Xylocopa appendiculata circumvolans revealed that the component profile of this venom mostly consisted of small peptides. The major peptide components, xylopin and xylopinin, were purified and characterized in a conventional manner. Their chemical and biological characteristics, belonging to linear cationic -helical peptides, are similar to the known solitary bee venom peptides, melectin and osmin. Pore formation in artificial lipid bilayers was demonstrated for the first time with a solitary bee peptide.

Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans

Background: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS. Methods: A reverse-phase HPLC connected to ESI-OrbiTrap MS was used for LC-MS. On-line mass fingerprinting was made from TIC, and data-dependent tandem mass spectrometry gave MSMS spectra. A major peptide component was isolated by reverse-phase HPLC by conventional way, and its sequence was determined by Edman degradation, which was finally corroborated by solid phase synthesis. Using the synthetic specimen, biological activities (antimicrobial activity, mast cell devaluation, hemolysis, leishmanicidal activity) and pore formation in artificial lipid bilayer were evaluated. Results: On-line mass fingerprinting revealed that the crude venom contained 124 components. MS/MS analysis gave 75 full sequences of the peptide components. Most of these are related to the major and novel peptide, xylopin. Its sequence, GFVALLKKLPLILKHLH-NH2, has characteristic features of linear cationic α-helical peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, it can be predicted to adopt an amphipathic α-helix secondary structure. In biological evaluation, xylopin exhibited broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. Additionally, the peptide was able to incorporate pores in artificial lipid bilayers of azolectin, confirming the mechanism of the cytolytic activity by pore formation in biological membranes. Conclusions: LC-ESI-MS and MS/MS analysis of the crude venom extract from a solitary bee Xylocopa appendiculata circumvolans revealed that the component profile of this venom mostly consisted of small peptides. The major peptide components, xylopin and xylopinin, were purified and characterized in a conventional manner. Their chemical and biological characteristics, belonging to linear cationic α-helical peptides, are similar to the known solitary bee venom peptides, melectin and osmin. Pore formation in artificial lipid bilayers was demonstrated for the first time with a solitary bee peptide.(AU)

Peptidomic analysis of the venom of the solitary bee Xylocopa appendiculata circumvolans

Background: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS. Methods: A reverse-phase HPLC connected to ESI-OrbiTrap MS was used for LC-MS. On-line mass fingerprinting was made from TIC, and data-dependent tandem mass spectrometry gave MSMS spectra. A major peptide component was isolated by reverse-phase HPLC by conventional way, and its sequence was determined by Edman degradation, which was finally corroborated by solid phase synthesis. Using the synthetic specimen, biological activities (antimicrobial activity, mast cell devaluation, hemolysis, leishmanicidal activity) and pore formation in artificial lipid bilayer were evaluated. Results: On-line mass fingerprinting revealed that the crude venom contained 124 components. MS/MS analysis gave 75 full sequences of the peptide components. Most of these are related to the major and novel peptide, xylopin. Its sequence, GFVALLKKLPLILKHLH-NH2, has characteristic features of linear cationic α-helical peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, it can be predicted to adopt an amphipathic α-helix secondary structure. In biological evaluation, xylopin exhibited broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. Additionally, the peptide was able to incorporate pores in artificial lipid bilayers of azolectin, confirming the mechanism of the cytolytic activity by pore formation in biological membranes. Conclusions: LC-ESI-MS and MS/MS analysis of the crude venom extract from a solitary bee Xylocopa appendiculata circumvolans revealed that the component profile of this venom mostly consisted of small peptides. The major peptide components, xylopin and xylopinin, were purified and characterized in a conventional manner. Their chemical and biological characteristics, belonging to linear cationic α-helical peptides, are similar to the known solitary bee venom peptides, melectin and osmin. Pore formation in artificial lipid bilayers was demonstrated for the first time with a solitary bee peptide.(AU)