Testicular biometry and hierarchy of dominance of the white-lipped peccary (Mammalia, Tayassuidae) / Biometria testicular e hierarquia de dominância do queixada (Mammalia, Tayassuidae)

The objective of this study was to obtain data on the testicular biometry of the white-lipped peccary (Tayassu pecari) and to verify if there is a correlation between the individual's biometric data with their dominance rank. Data were collected from 16 adult males, aged between two and seven years, who made up the same group. By the agonistic interactions analyses we calculated the linearity indexes (h') of the dominance hierarchy and dominance rank. At the end of the behavioral observations, males were captured to collect biometric data from the testis. White-lipped peccaries showed testicles located in a perineal position, inclined cranio-ventrally with oval shape, flattened laterally and with tenso-elastic consistency (2.54±0.07). There was variance in the means (± standard deviation) of testis length (5.88±1.05cm), width (4.24 ± 0.98cm), height (4.44±0.86cm), and total scrotal width (8.78±17.05). The linear dominance hierarchy described the social structure of the males of this group (h'>0.9), with probability of linearity in the hierarchy greater than chance (P = 0.02). There was a correlation between dominance rank with the body mass, length and volume of the testicles. As in general the dimensions of the testicles are directly correlated with sperm production and also testosterone, this characteristic favors the reproductive performance of dominant white-lips males. Therefore, the characteristics of testicular biometry of white-lips should be considered for the selection of individuals more likely to reproduce in captivity favoring the conservation of this vulnerable species.(AU)

O objetivo neste estudo foi obter dados sobre a biometria testicular do queixada (Tayassu pecari) e verificar se há correlação entre os dados biométricos do indivíduo com seu posto de dominância. Os dados foram coletados de 16 queixadas adultos, com idades entre dois e sete anos, que compunham o mesmo grupo. Por meio de análises das interações agonísticas foram calculados os índices de linearidade (h') da hierarquia de dominância e determinado o rank dos indivíduos. Ao final das observações comportamentais, os machos foram capturados para coleta de dados biométricos do testículo. Os queixadas apresentaram testículos localizados em posição perineal, inclinados cranioventralmente com formato oval, achatados láterolateralmente e com consistência tensoelástica (2,54±0,07). Houve variação nas médias (± desvio padrão) do comprimento testicular (5,88±1,05cm), largura (4,24±0,98cm), altura (4,44±0,86cm) e largura escrotal total (8,78±17,05). A hierarquia de dominância linear descreveu a estrutura social dos machos desse grupo (h'>0,9), com probabilidade de linearidade na hierarquia maior que o acaso (P = 0,02). Houve correlação entre a classificação de dominância com a massa corporal, comprimento e volume dos testículos. Como em geral as dimensões dos testículos estão diretamente correlacionadas com a produção de esperma e também de testosterona, essa característica favorece o desempenho reprodutivo de machos de queixadas brancos dominantes. Portanto, as características da biometria testicular de queixadas devem ser consideradas para a seleção de indivíduos com maior probabilidade de reprodução em cativeiro favorecendo a conservação desta espécie vulnerável.(AU)

Body and testicular biometry of Crioulo stallions / Biometria corporal e testicular de garanhões da raça Crioula

Brazilian Association of Crioulo Horse Breeders (ABCCC) establishes minimal and maximal body biometrics evaluation; although, nothing is mentioned about testicular measurements. Body and testicular growth are associated and related to age. We described data regarding body and testicular biometry of Crioulo stallions, with a comparative study between young and adult categories. Evaluation was performed in 56 stallions, Crioulo breed, split in: youngsters (3 and 4 years-old, n=16) and adults (above 5 years-old, n=40). Body biometry included weight, height, cannon bone and chest circumference, body score condition and neck fat accumulation. Testicular biometry included height, length, width, volume, combined volume and daily sperm output (DSO). Statistical analyses included descriptive statistic, Pearson correlation, comparison between means by Kruskal Wallis, being P < 0.05 considered significant. There was no significant difference between the parameters of body and testicular biometrics between categories young and adult. In relation to height, the average was very close to the lower limit established, while chest and cannon bone circumference were above the minimum recommended by ABCCC. Cannon bone circumference presented a positive correlation with height. Most of stallions presented excessive body fat, with a body score condition above 8 (scale 1-9). Neck fat accumulation presented a positive correlation with body score condition. Testicular height, length, width and volume presented a positive correlation between the ipsi and contralateral testicle, total testicular volume and DSO. In conclusion, no difference in the body and testicular biometric evaluation was observed between young and adult Crioulo stallions. Testicular and body growth are associated and also related to age, so our finding suggested that after 3-4 years-old most of Crioulo stallions have already reached maximum growth.

A Associação Brasileira de Criadores de Cavalos Crioulos (ABCCC) define parâmetros mínimos e máximos para algumas aferições biométricas no corpo do animal, embora nada seja mencionado sobre medidas testiculares. O crescimento corporal e testicular estão associados e relacionados a idade do animal. Objetivamos descrever parâmetros relacionados a biometria corporal e testicular de garanhões Crioulos, comparando o grupo de jovens e adultos. Foi realizada avaliação em 56 garanhões Crioulos, divididos em: jovens (três e quatro anos, n=16) e adultos (acima de cinco anos, n=40). A biometria corporal incluiu peso, altura, circunferência de canela, perímetro torácico, escore corporal e acúmulo de gordura no pescoço. A biometria testicular incluiu altura, comprimento, largura, volume de cada testículo, volume combinado dos testículos e estimativa de produção diária de espermatozoides (DSO). Com os dados, foi realizada estatística descritiva, correlação de Pearson, comparação entre médias com Kruskal Wallis, sendo considerado significativo quando P < 0,05. Não foi observada diferença significativa entre as avaliações biométricas e testiculares de garanhões das categorias jovens e adultos. Em relação à altura, a média foi muito próxima ao limite inferior estabelecido, enquanto a circunferência torácica e de canela estavam acima do limite mínimo recomendado pela ABCCC. A circunferência de canela apresentou correlação positiva com altura. A maioria dos garanhões apresentou alta deposição de gordura, com condição corporal acima de 8 (escala 1-9). O acúmulo de gordura no pescoço apresentou correlação positiva com escore corporal. A altura, largura, comprimento e volume testicular apresentaram correlação positiva com testículo ipsi e contralateral, volume testicular total e DSO. Em conclusão, não houve diferença entre dados de biometria corporal e testicular de garanhões Crioulos adultos e jovens. O crescimento corporal e testicular estão associados e relacionados a idade, sendo assim, nossos resultados sugerem que após 3-4 anos de idade, a maioria dos garanhões Crioulos já atingiu seu pico de desenvolvimento.

Quais são as dúvidas no momento do exame clínico andrológico bovino? / What are the doubts at the time of the bovine andrological clinical examination?

O exame clínico andrológico é a ferramenta de eleição utilizada para avaliar a fertilidade de um reprodutor. Normalmente utilizado em tourinhos jovens na pré-comercialização, como uma forma de garantia reprodutiva; no período pré-estação de monta; quando existem falhas ou desordens reprodutivas nos rebanhos; prévio ao ingresso nas centrais de inseminação; na determinação da puberdade, dentre outras. O exame clínico andrológico deve ser realizado por profissional com experiência suficiente para tomada de decisões sobre o real potencial reprodutivo dos animais, porém, em muitas ocasiões mesmo um profissional capacitado defronta-se com dúvidas, entre elas destacam-se as questões relacionadas ao valor cobrado e "tipo de exame"; a biometria testicular; exigências relacionadas a impotencia coeundi; e o que é necessário no certificado do exame. Buscou-se elencar, explanar e discutir essas questões em vista de diminuí-las e/ou encontrar possíveis soluções. O exame clínico andrológico é uma etapa fundamental e complexa para a seleção de reprodutores, e apesar de constantemente debatida, ainda permanecem lacunas a serem respondidas, visto que os profissionais necessitam de constante atualização.(AU)

The clinical andrological examination is the tool of choice used to assess the fertility of a sire. Usually used in young bulls in pre-marketing, as a form of reproductive guarantee; in the pre-mounting season period; when there are reproductive failures/disorders in herds; before entering insemination centers, in determining puberty, among others. The clinical andrological examination must be carried out by a professional with sufficient expertise to make decisions about the real reproductive potential of these animals, however, on many occasions even a trained professional is faced with doubts, among them the issues related to the amount charged and "type of exam"; testicular biometry; requirements related to impotencia coeundi; and what is required in the exam certificate. Based on the above context, we sought to list some possible doubts, explaining them and discussing them among veterinarians, so that such issues could be reduced and/or possible alternative solutions could be defined. The clinical andrological examination is a fundamental and complex step for the selection of sires, and despite being constantly debated, there are still gaps to be answered, since professionals need constant updating.(AU)

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

Abstract The objective of this study was to evaluate the testicular biometric, seminal, and plasma testosterone levels in lambs subjected to an anti-GnRH vaccine as a method of castration. Thirty entire, crossbred Santa Inês male lambs were randomly distributed into three treatment (T): T1 was the control group, with the administration of 1 mL of saline solution subcutaneously (SC); 1.0 and 0.5 mL of an anti-GnRH vaccine were administered SC in T2 and T3, respectively. Testicular biometric variables, physical and morphological variables of semen, and plasma testosterone concentrations were evaluated. At D60, there was a reduction in testicular length, width, thickness, and scrotal circumference of the immunocastrated animals regardless of the vaccine dose used (P < 0.05). A reduction in semen physical variables at both dosages (P < 0.05) was observed, with azoospermia, in 80% and 70% of animals in the T2 and T3 groups, respectively. At D60, the immunocastrated animals also showed an increase in spermatozoa defects (P < 0.05), whereas plasma testosterone concentration decreased (P < 0.05). Immunocastration of lambs using the Bopriva vaccine at doses of 1.0 and 0.5 mL is efficient in inducing azoospermia in up to 80% of animals, although two doses in a 30-day interval are necessary for it to be an effective and safe method. Efficacy was demonstrated through a reduction in serum testosterone levels, testicular biometry, and seminal fluid analysis. Considering the efficacy of both doses in this study, we recommend using the lower dose (0.5 mL), which will allow for a 50% reduction in vaccine costs.

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

The objective of this study was to evaluate the testicular biometric, seminal, and plasma testosterone levels in lambs subjected to an anti-GnRH vaccine as a method of castration. Thirty entire, crossbred Santa Inês male lambs were randomly distributed into three treatment (T): T1 was the control group, with the administration of 1 mL of saline solution subcutaneously (SC); 1.0 and 0.5 mL of an anti-GnRH vaccine were administered SC in T2 and T3, respectively. Testicular biometric variables, physical and morphological variables of semen, and plasma testosterone concentrations were evaluated. At D60, there was a reduction in testicular length, width, thickness, and scrotal circumference of the immunocastrated animals regardless of the vaccine dose used (P < 0.05). A reduction in semen physical variables at both dosages (P < 0.05) was observed, with azoospermia, in 80% and 70% of animals in the T2 and T3 groups, respectively. At D60, the immunocastrated animals also showed an increase in spermatozoa defects (P < 0.05), whereas plasma testosterone concentration decreased (P < 0.05). Immunocastration of lambs using the Bopriva vaccine at doses of 1.0 and 0.5 mL is efficient in inducing azoospermia in up to 80% of animals, although two doses in a 30-day interval are necessary for it to be an effective and safe method. Efficacy was demonstrated through a reduction in serum testosterone levels, testicular biometry, and seminal fluid analysis. Considering the efficacy of both doses in this study, we recommend using the lower dose (0.5 mL), which will allow for a 50% reduction in vaccine costs.(AU)

Testicular features of semi-confined Nellore bullssubjected to immunocastration / Características testiculares de touros Nelore semiconfinadossubmetidos a imunocastração

This study aimed to evaluate the effect of immunocastration on the scrotal circumference, as well as in the macroscopic and microscopic testicular features in Nellore bulls bred under a semi-confining system for 91 days. Sixty animals were divided into two groups: 30 intact animals (non-vaccinated) and 30 immunocastrated animals. The immunocastrated animals were treated with two anti-GnRH vaccine doses (BoprivaTM - Zoetis Australia Pty Ltd). The scrotal circumference (SC) was measured on days 0 and 56. Testicular parameters: biometry traits (length, width, and height ­ cm; volume ­ cm3) and weight (g) was measured on day 91. Three testicles from each group were histologically processed to find the seminiferous tubule diameter and epithelium thickness (µm). Data were analyzed in the Minitab® 19 statistical software. The macroscopic features (SC, and testes weight, volume, length, width, and height) and the microscopic features (seminiferous tubule diameter and epithelium thickness) were compared between the two groups through Student's t-test and Mann-Whitney test. All the values were lower (p<0.05) in animals immunized against GnRH, except for the right testis width and seminiferous tubule diameter. Results indicate that immunization against GnRH affected testicular development.(AU)

O objetivo do presente estudo foi avaliar o efeito da imunocastração no perímetro escrotal, assim como nas características testiculares macroscópicas e microscópicas de touros Nelore criados em um sistema de semiconfinamento por 91 dias. Sessenta animais foram divididos em dois grupos: 30 animais inteiros (não vacinados) e 30 imunocastrados. Os animais imunocastrados receberam duas doses de uma vacina anti-GnRH (Bopriva® - Zoetis Ltda). O perímetro escrotal (PE) foi mensurado nos dias 0 e 56. Parâmetros testiculares: atributos biométricos (comprimento, largura e altura ­ cm; volume cm3) e peso (g) foram medidos no dia 91. Três testículos de cada grupo foram processados para histologia clássica a fim de avaliar o diâmetro e a espessura do epitélio dos túbulos seminíferos (µm). Os dados foram analisados pelo software estatístico Minitab® 19. As médias das características macroscópicas (PE, peso, volume, comprimento, largura e altura dos testículos) e das microscópicas (diâmetro e espessura do epitélio dos túbulos seminíferos) foram comparadas entre os dois grupos pelos testes t de Student e Mann-Whitney. Todos os valores foram menores (p<0,05) em animais imunizados contra o GnRH, exceto a altura média do testículo direito e o diâmetro do túbulo seminífero. Os resultados indicam que a imunização contra o GnRH afeta o desenvolvimento testicular.(AU)

Testicular features of semi-confined Nellore bullssubjected to immunocastration / Características testiculares de touros Nelore semiconfinadossubmetidos a imunocastração

This study aimed to evaluate the effect of immunocastration on the scrotal circumference, as well as in the macroscopic and microscopic testicular features in Nellore bulls bred under a semi-confining system for 91 days. Sixty animals were divided into two groups: 30 intact animals (non-vaccinated) and 30 immunocastrated animals. The immunocastrated animals were treated with two anti-GnRH vaccine doses (BoprivaTM - Zoetis Australia Pty Ltd). The scrotal circumference (SC) was measured on days 0 and 56. Testicular parameters: biometry traits (length, width, and height ­ cm; volume ­ cm3) and weight (g) was measured on day 91. Three testicles from each group were histologically processed to find the seminiferous tubule diameter and epithelium thickness (µm). Data were analyzed in the Minitab® 19 statistical software. The macroscopic features (SC, and testes weight, volume, length, width, and height) and the microscopic features (seminiferous tubule diameter and epithelium thickness) were compared between the two groups through Student's t-test and Mann-Whitney test. All the values were lower (p<0.05) in animals immunized against GnRH, except for the right testis width and seminiferous tubule diameter. Results indicate that immunization against GnRH affected testicular development.(AU)

O objetivo do presente estudo foi avaliar o efeito da imunocastração no perímetro escrotal, assim como nas características testiculares macroscópicas e microscópicas de touros Nelore criados em um sistema de semiconfinamento por 91 dias. Sessenta animais foram divididos em dois grupos: 30 animais inteiros (não vacinados) e 30 imunocastrados. Os animais imunocastrados receberam duas doses de uma vacina anti-GnRH (Bopriva® - Zoetis Ltda). O perímetro escrotal (PE) foi mensurado nos dias 0 e 56. Parâmetros testiculares: atributos biométricos (comprimento, largura e altura ­ cm; volume cm3) e peso (g) foram medidos no dia 91. Três testículos de cada grupo foram processados para histologia clássica a fim de avaliar o diâmetro e a espessura do epitélio dos túbulos seminíferos (µm). Os dados foram analisados pelo software estatístico Minitab® 19. As médias das características macroscópicas (PE, peso, volume, comprimento, largura e altura dos testículos) e das microscópicas (diâmetro e espessura do epitélio dos túbulos seminíferos) foram comparadas entre os dois grupos pelos testes t de Student e Mann-Whitney. Todos os valores foram menores (p<0,05) em animais imunizados contra o GnRH, exceto a altura média do testículo direito e o diâmetro do túbulo seminífero. Os resultados indicam que a imunização contra o GnRH afeta o desenvolvimento testicular.(AU)

Testicular and seminal evaluation of goats fed hay Cenostigma pyramidale / Avaliação testicular e seminal de caprinos alimentados com feno de Cenostigma pyramidale

This study aimed to assess the possible occurrence of reproductive changes in male goats associated with ingestion of Cenostigma pyramidale hay. Sixteen animals divided into two experimental groups, G1 and G2 (control group) were used. Animals in G1 received 2% of forage, based on live weight (LW), composed of 100% of C. pyramidale, and animals in G2 received 2% of Panicum maximum "Massai' grass hay, based on LW. Both groups received 1% of concentrated feed supplementation based on LW, along with mineralized salt and water ad libitum. The goats were subjected to weighing, testicular biometry, and semen and blood collection every 30 days. After 120 days, the animals were castrated and their testes were collected. Testicular measurements were performed and fragments were collected for histological processing to determine the gonadosomatic index (GSI), diameter of the seminiferous tubules, height of the germinal epithelium (HGE), volumetric proportion and volume of the testicular parenchyma components, total length of the seminiferous tubules, length of the seminiferous tubules per gram of testis, and leydigosomatic and tubulosomatic indexes. The data were evaluated for normality using the Student's t-test. Data with normal distribution were assessed using analysis of variance (ANOVA) and the non-parametric data were evaluated using the Kruskal-Wallis test, both at 5% probability. Statistically significant differences (p<0.05) were observed for GSI (G1=0.48 ±0.08 and G2=0.34 ±0.09) and HGE (G1=52.95 ±2.99 and G2=.47.63 ±2.67) between treatments. Consumption of C. pyramidale hay increased LW and, consequently, testicular weight, contributing to high GSI. In conclusion, ingestion of C. pyramidale has no toxic effect on the testicular, seminal and histological parameters of goat testis. Due to its nutritional characteristics, consumption of this plant improves animal body development. Because C. pyramidale is adapted to semi-arid regions, it can be an alternative source of feed for goats during periods of shortage.(AU)

Para avaliar a possível ocorrência de alterações reprodutivas em caprinos machos associado ao consumo de Cenostigma pyramidale, foram utilizados 16 animais divididos em dois grupos experimentais, G1 e G2 (grupo controle). Animais pertencentes ao G1 receberam 2% de volumoso, com base no peso vivo (PV), constituído de 100% de C. pyramidale e o G2 receberam 2%, com base no PV, de feno de Panicum maximum 'Massai'. Todos os grupos receberam 1%, com base no PV, de suplementação concentrada, além de sal mineralizado e água ad libitum. A cada 30 dias os animais eram submetidos à pesagem, biometria testicular e coletas de sêmen. O sêmen foi avaliado quanto ao volume, turbilhonamento, vigor, motilidade, concentração espermática, defeitos maiores, menores e totais. Após 120 dias os animais foram castrados e os testículos coletados. Foram realizadas as mensurações testiculares e coletados fragmentos para o processamento histológico, para determinação do índice gonadossomático, diâmetro dos túbulos seminíferos, altura do epitélio germinativo, proporção volumétrica e volume dos componentes do parênquima testicular, comprimento total dos túbulos seminíferos, comprimento de túbulo seminífero por grama de testículo, índices leydigossomático e tubulossomático. Os dados foram avaliados quanto à normalidade pelo teste t de Student, os dados com distribuição normal foram analisados por análise de variância com 5% de probabilidade e os não paramétricos, pelo teste de Kruskal-Wallis, a 5% de probabilidade. Houve diferenças significativas (p<0,05) para índice gonadossomático (G1=0,48±0,08 e G2=0,34±0,09) e altura do epitélio germinativo (G1=52,95±2,99 e G2=47,63±2,67) entre os tratamentos. O feno de catingueira promoveu aumento no peso corporal e consequentemente maior peso testicular, o que contribui para elevação do IGS. Concluiu-se que o consumo da C. pyramidale não possui efeito tóxico sobre os parâmetros testiculares, seminais e histológicos do testículo dos caprinos e que a planta, por suas características nutricionais promoveu melhora no desenvolvimento corporal dos animais e por ser uma planta adaptada ao clima do semiárido, se constitui uma fonte alternativa de alimentação para esta categoria animal, durante períodos de escassez de alimento.(AU)

Testicular and seminal evaluation of goats fed hay Cenostigma pyramidale / Avaliação testicular e seminal de caprinos alimentados com feno de Cenostigma pyramidale

This study aimed to assess the possible occurrence of reproductive changes in male goats associated with ingestion of Cenostigma pyramidale hay. Sixteen animals divided into two experimental groups, G1 and G2 (control group) were used. Animals in G1 received 2% of forage, based on live weight (LW), composed of 100% of C. pyramidale, and animals in G2 received 2% of Panicum maximum "Massai' grass hay, based on LW. Both groups received 1% of concentrated feed supplementation based on LW, along with mineralized salt and water ad libitum. The goats were subjected to weighing, testicular biometry, and semen and blood collection every 30 days. After 120 days, the animals were castrated and their testes were collected. Testicular measurements were performed and fragments were collected for histological processing to determine the gonadosomatic index (GSI), diameter of the seminiferous tubules, height of the germinal epithelium (HGE), volumetric proportion and volume of the testicular parenchyma components, total length of the seminiferous tubules, length of the seminiferous tubules per gram of testis, and leydigosomatic and tubulosomatic indexes. The data were evaluated for normality using the Student's t-test. Data with normal distribution were assessed using analysis of variance (ANOVA) and the non-parametric data were evaluated using the Kruskal-Wallis test, both at 5% probability. Statistically significant differences (p<0.05) were observed for GSI (G1=0.48 ±0.08 and G2=0.34 ±0.09) and HGE (G1=52.95 ±2.99 and G2=.47.63 ±2.67) between treatments. Consumption of C. pyramidale hay increased LW and, consequently, testicular weight, contributing to high GSI. In conclusion, ingestion of C. pyramidale has no toxic effect on the testicular, seminal and histological parameters of goat testis. Due to its nutritional characteristics, consumption of this plant improves animal body development. Because C. pyramidale is adapted to semi-arid regions, it can be an alternative source of feed for goats during periods of shortage.(AU)

Para avaliar a possível ocorrência de alterações reprodutivas em caprinos machos associado ao consumo de Cenostigma pyramidale, foram utilizados 16 animais divididos em dois grupos experimentais, G1 e G2 (grupo controle). Animais pertencentes ao G1 receberam 2% de volumoso, com base no peso vivo (PV), constituído de 100% de C. pyramidale e o G2 receberam 2%, com base no PV, de feno de Panicum maximum 'Massai'. Todos os grupos receberam 1%, com base no PV, de suplementação concentrada, além de sal mineralizado e água ad libitum. A cada 30 dias os animais eram submetidos à pesagem, biometria testicular e coletas de sêmen. O sêmen foi avaliado quanto ao volume, turbilhonamento, vigor, motilidade, concentração espermática, defeitos maiores, menores e totais. Após 120 dias os animais foram castrados e os testículos coletados. Foram realizadas as mensurações testiculares e coletados fragmentos para o processamento histológico, para determinação do índice gonadossomático, diâmetro dos túbulos seminíferos, altura do epitélio germinativo, proporção volumétrica e volume dos componentes do parênquima testicular, comprimento total dos túbulos seminíferos, comprimento de túbulo seminífero por grama de testículo, índices leydigossomático e tubulossomático. Os dados foram avaliados quanto à normalidade pelo teste t de Student, os dados com distribuição normal foram analisados por análise de variância com 5% de probabilidade e os não paramétricos, pelo teste de Kruskal-Wallis, a 5% de probabilidade. Houve diferenças significativas (p<0,05) para índice gonadossomático (G1=0,48±0,08 e G2=0,34±0,09) e altura do epitélio germinativo (G1=52,95±2,99 e G2=47,63±2,67) entre os tratamentos. O feno de catingueira promoveu aumento no peso corporal e consequentemente maior peso testicular, o que contribui para elevação do IGS. Concluiu-se que o consumo da C. pyramidale não possui efeito tóxico sobre os parâmetros testiculares, seminais e histológicos do testículo dos caprinos e que a planta, por suas características nutricionais promoveu melhora no desenvolvimento corporal dos animais e por ser uma planta adaptada ao clima do semiárido, se constitui uma fonte alternativa de alimentação para esta categoria animal, durante períodos de escassez de alimento.(AU)

Biometria testicular e sua influência no comportamento sexual de búfalos (Bubalus buballis) / Testicular parameters and its influence in buffaloes (Bubalus buballis) sexual behaviour

Background: Brazil is one of the greatest bovine meat exporter in the world. However, the technologies applied at buffalo’s reproduction is not specific for this specie, adapted technics were established from the cattle breed. When the animalshows weight gain, gonadal and behavioral physiology fallow this tendency and the reproductive status become good andsatisfactory. Thus, the aim of this study was to compare the reproduction characteristics of male buffaloes and its testicularparameters with sexual behavior.Materials, Methods & Results: Seven males Murrah breed with 67.29 ± 11.4 months of age were maintained at confinement for artificial shading. The testicular parameter was performed in restraint trunk and the sexual behavior evaluationwas done with an estrus female. The sexual behavior was performed with an estrous female as a dummy. The testicularbiometry was measured at the beginning and at the end of the experimental design, with the follow parameters: length,width, depth/thickness, scrotal circumference and the total volume. The data were submitted to the analysis of variance,and the means was compared with the Student-Newman-Keuls test, with significance difference P < 0.05 between themedians. The differences in the parameters were scrotal circumference (23.36 cm vs 24.86 cm), testicular length (8.71 cmvs 9.77 cm), right testicular width (8.57 cm vs 9.53 cm) and testicular volume (1.627.40 cm3 vs 2.149,68 cm3), respectively.The sexual behavior showed an increase in the Flehmen reflex, mounts...

Biometria testicular e sua influência no comportamento sexual de búfalos (Bubalus buballis) / Testicular parameters and its influence in buffaloes (Bubalus buballis) sexual behaviour

Background: Brazil is one of the greatest bovine meat exporter in the world. However, the technologies applied at buffalos reproduction is not specific for this specie, adapted technics were established from the cattle breed. When the animalshows weight gain, gonadal and behavioral physiology fallow this tendency and the reproductive status become good andsatisfactory. Thus, the aim of this study was to compare the reproduction characteristics of male buffaloes and its testicularparameters with sexual behavior.Materials, Methods & Results: Seven males Murrah breed with 67.29 ± 11.4 months of age were maintained at confinement for artificial shading. The testicular parameter was performed in restraint trunk and the sexual behavior evaluationwas done with an estrus female. The sexual behavior was performed with an estrous female as a dummy. The testicularbiometry was measured at the beginning and at the end of the experimental design, with the follow parameters: length,width, depth/thickness, scrotal circumference and the total volume. The data were submitted to the analysis of variance,and the means was compared with the Student-Newman-Keuls test, with significance difference P < 0.05 between themedians. The differences in the parameters were scrotal circumference (23.36 cm vs 24.86 cm), testicular length (8.71 cmvs 9.77 cm), right testicular width (8.57 cm vs 9.53 cm) and testicular volume (1.627.40 cm3 vs 2.149,68 cm3), respectively.The sexual behavior showed an increase in the Flehmen reflex, mounts...(AU)

Morphometric measurements of sheep fed with increasing levels of sunflower meal

The objective of this work was to evaluate the performance, body morphometric measurements and testicular development in lambs fed with different rates of sunflower meal inclusion. The animals (24) were randomly distributed in treatments with 0, 10, 20 and 30% of sunflower meal in the dry matter of the diet, in six replicates, during 56 days of confinement. The addition of the coproduct at increasing dietary rates increased linearly the dry matter intake in the percentage of live weight and neutral detergent fiber. Despite this, there was a linear reduction in ether extract intake and chest width. On the other hand, there was no difference in daily, total and final weight gains. The body condition score; height of withers, croup and thorax; length of body and croup; width of fore croup, hind croup and chest; thoracic and neck diameters also did not change with the addition of the coproduct. The testicular measures 26.76; 6.11; 6.08; 5.22; 5.21; 4.80, and 4.81cm varied quadratically with the inclusion. Therefore, the inclusion of 30% of the coproduct changes nutrient intake and testicular biometry, but does not interfere in the body weight and development, being an economical alternative.(AU)

Morphometric measurements of sheep fed with increasing levels of sunflower meal

The objective of this work was to evaluate the performance, body morphometric measurements and testicular development in lambs fed with different rates of sunflower meal inclusion. The animals (24) were randomly distributed in treatments with 0, 10, 20 and 30% of sunflower meal in the dry matter of the diet, in six replicates, during 56 days of confinement. The addition of the coproduct at increasing dietary rates increased linearly the dry matter intake in the percentage of live weight and neutral detergent fiber. Despite this, there was a linear reduction in ether extract intake and chest width. On the other hand, there was no difference in daily, total and final weight gains. The body condition score; height of withers, croup and thorax; length of body and croup; width of fore croup, hind croup and chest; thoracic and neck diameters also did not change with the addition of the coproduct. The testicular measures 26.76; 6.11; 6.08; 5.22; 5.21; 4.80, and 4.81cm varied quadratically with the inclusion. Therefore, the inclusion of 30% of the coproduct changes nutrient intake and testicular biometry, but does not interfere in the body weight and development, being an economical alternative.

Classificação andrológica por pontos e características andrológicas na avaliação reprodutiva de touros da raça Gir candidatos ao teste de progênie / Andrological points classification and andrological reproductive evaluation characteristics in Gir bulls candidates for progenietest

A eficiência reprodutiva deve ser avaliada em propriedades leiteiras para identificação de indivíduos com problemas reprodutivos ligados tanto à fêmea quanto ao macho, visando minimizar perdas na produção. A reprodução está entre os fatores que afetam a produtividade dos rebanhos e chama atenção, em alguns momentos, pelos baixos índices na pecuária tradicional. Objetivou-se estudar a ferramenta de avaliação espermática denominada índice CAP e sua correlação com as análises biométricas e morfológicas do sêmen de touros da raça Gir. Foram utilizados 34 touros da raça Gir (Bos taurus indicus) aptidão leiteira, hígidos, com 17 a 31 meses de idade e 450 kg de massa corporal média. Os touros foram submetidos a exame andrológico (avaliação física incluindo biometria testicular e morfológica do sêmen), seguido pela classificação andrológica por pontos (índice CAP). Foram realizadas três coletas independentes para avaliar a qualidade espermática por touro durante o período do experimento. De acordo com a avaliação do índice CAP, os animais foram classificados como excelentes (17 %) e muito bons (83 %). O índice CAP correlacionou-se positivamente com o perímetro escrotal e as características físicas do sêmen.

Reproductive efficiency should be evaluated in dairy farms, when they are related to reproductive problems in both female and male. Breeding is among factors that affect herd productivity and draws attention by low rates of traditional livestock breeding. The aim was to study sperm evaluation tool known as the CAP index and its correlation with the biometric and morphological analyzes of Gir bulls semen. Thirty four healthy Gir bulls (Bos taurus indicus), dairy fitness, with 17 to 31 months of age with 450 kg of body mass were used. The bulls were submitted to andrological examination (physical evaluation including testicular biometry and semen morphological analysis) follow by andrological classification by points (CAP index). Three independent collections were carried out to evaluate sperm quality per bull during the experiment period According to the CAP index, animals were classified as excellent (17%) and as very good (83%). The CAP Index correlated positively with scrotal perimeter and semen physical characteristics.

Classificação andrológica por pontos e características andrológicas na avaliação reprodutiva de touros da raça Gir candidatos ao teste de progênie / Andrological points classification and andrological reproductive evaluation characteristics in Gir bulls candidates for progenietest

A eficiência reprodutiva deve ser avaliada em propriedades leiteiras para identificação de indivíduos com problemas reprodutivos ligados tanto à fêmea quanto ao macho, visando minimizar perdas na produção. A reprodução está entre os fatores que afetam a produtividade dos rebanhos e chama atenção, em alguns momentos, pelos baixos índices na pecuária tradicional. Objetivou-se estudar a ferramenta de avaliação espermática denominada índice CAP e sua correlação com as análises biométricas e morfológicas do sêmen de touros da raça Gir. Foram utilizados 34 touros da raça Gir (Bos taurus indicus) aptidão leiteira, hígidos, com 17 a 31 meses de idade e 450 kg de massa corporal média. Os touros foram submetidos a exame andrológico (avaliação física incluindo biometria testicular e morfológica do sêmen), seguido pela classificação andrológica por pontos (índice CAP). Foram realizadas três coletas independentes para avaliar a qualidade espermática por touro durante o período do experimento. De acordo com a avaliação do índice CAP, os animais foram classificados como excelentes (17 %) e muito bons (83 %). O índice CAP correlacionou-se positivamente com o perímetro escrotal e as características físicas do sêmen.(AU)

Reproductive efficiency should be evaluated in dairy farms, when they are related to reproductive problems in both female and male. Breeding is among factors that affect herd productivity and draws attention by low rates of traditional livestock breeding. The aim was to study sperm evaluation tool known as the CAP index and its correlation with the biometric and morphological analyzes of Gir bulls semen. Thirty four healthy Gir bulls (Bos taurus indicus), dairy fitness, with 17 to 31 months of age with 450 kg of body mass were used. The bulls were submitted to andrological examination (physical evaluation including testicular biometry and semen morphological analysis) follow by andrological classification by points (CAP index). Three independent collections were carried out to evaluate sperm quality per bull during the experiment period According to the CAP index, animals were classified as excellent (17%) and as very good (83%). The CAP Index correlated positively with scrotal perimeter and semen physical characteristics.(AU)

Biometric testicular and serum testosterone concentration of Brazilian Ponies stallions / Biometria testicular e concentrações séricas de testosterona de garanhões Pôneis da raça Brasileira

Evaluation of the testis size is very important in stallions selection, since it is an indirect determinant of their reproductive potential. The aim of this study was to evaluate the biometric testicular traits, calculate the volume testicular and analyze the serum testosterone concentration of the stallion. During six months were measured the tests of adults Brazilian Ponies stallions from northern of Rio de Janeiro State, Brazil. The length, the width and the height of the testicles (TL, TW and TH) were measured. From these measurements, the total testicular volume (TTV) was calculated. The serum testosterone concentration was also evaluated. The data were studied by analysis of variance and were calculated the correlations among the traits. The average of the testis biometry were TL 5.8 ± 0.6 cm; TW 3.8 ± 0.5 cm; TH 4.7 ± 0.4 cm and 111.2 ± 27.7 for TTV. Mean of serum testosterone concentration was 0.89 ± 0.52 ng/dl. High correlations between biometry testicular traits with testicular volume were observed and low to moderate correlations were observed for testicular traits, testosterone and sperm output. The testis size could be an important trait that assists to the prediction of stallion's reproductive potential of the Brazilian ponies stallions. It is suggested that there would be no fluctuation in serum testosterone concentration in Brazilian ponies stallions that coult afect on its fertility.(AU)

Avaliação do tamanho dos testículos é muito importante na seleção de garanhões, uma vez que é um determinante indireto do seu potencial reprodutivo. O objetivo deste estudo foi avaliar as características de biometria testiculares, calcular o volume testicular e analisar a concentração sérica de testosterona de garanhões. Durante seis meses, foram medidos os testículos de garanhões Pôneis da raça Brasileiro adultos, criados no Norte do Estado do Rio de Janeiro, Brasil. O comprimento a largura e a altura dos testículo (TL, TW e TH) foram mensurados. A partir dessas medidas, calculou-se o volume testicular total (TTV). Os dados foram avaliados por análise de variância e foram calculadas as correlações entre as características. A média geral das características de biometria testicular e volume testicular foram: TL 5,8 ± 0,6 cm; TW 3,8 ± 0,5 cm; TH 4,7 ± 0,4 cm e TTV 111.2 ± 27,7. A média de concentração sérica de testosterona foi de 0,89 ± 0,52 ng/dl. Altas correlações foram observadas entre as características de biometria testicular e volume testicular. Baixas a médias correlações entre as características testiculares com produção espermática e com concentração de testosterona foram observadas. O tamanho testicular pode ser uma importante característica que auxilia na predição do potencial reprodutivo do garanhão pôneis da raça Brasileira. Sugere-se que não há variações na concentração sérica de testosterona nos pôneis da raça Brasileira que poderia afetar em sua fertilidade.(AU)

Biometric testicular and serum testosterone concentration of Brazilian Ponies stallions / Biometria testicular e concentrações séricas de testosterona de garanhões Pôneis da raça Brasileira

Evaluation of the testis size is very important in stallions’ selection, since it is an indirect determinant of their reproductive potential. The aim of this study was to evaluate the biometric testicular traits, calculate the volume testicular and analyze the serum testosterone concentration of the stallion. During six months were measured the tests of adults Brazilian Ponies stallions from northern of Rio de Janeiro State, Brazil. The length, the width and the height of the testicles (TL, TW and TH) were measured. From these measurements, the total testicular volume (TTV) was calculated. The serum testosterone concentration was also evaluated. The data were studied by analysis of variance and were calculated the correlations among the traits. The average of the testis biometry were TL 5.8 ± 0.6 cm; TW 3.8 ± 0.5 cm; TH 4.7 ± 0.4 cm and 111.2 ± 27.7 for TTV. Mean of serum testosterone concentration was 0.89 ± 0.52 ng/dl. High correlations between biometry testicular traits with testicular volume were observed and low to moderate correlations were observed for testicular traits, testosterone and sperm output. The testis size could be an important trait that assists to the prediction of stallion's reproductive potential of the Brazilian ponies stallions. It is suggested that there would be no fluctuation in serum testosterone concentration in Brazilian ponies stallions that coult afect on its fertility.

Avaliação do tamanho dos testículos é muito importante na seleção de garanhões, uma vez que é um determinante indireto do seu potencial reprodutivo. O objetivo deste estudo foi avaliar as características de biometria testiculares, calcular o volume testicular e analisar a concentração sérica de testosterona de garanhões. Durante seis meses, foram medidos os testículos de garanhões Pôneis da raça Brasileiro adultos, criados no Norte do Estado do Rio de Janeiro, Brasil. O comprimento a largura e a altura dos testículo (TL, TW e TH) foram mensurados. A partir dessas medidas, calculou-se o volume testicular total (TTV). Os dados foram avaliados por análise de variância e foram calculadas as correlações entre as características. A média geral das características de biometria testicular e volume testicular foram: TL 5,8 ± 0,6 cm; TW 3,8 ± 0,5 cm; TH 4,7 ± 0,4 cm e TTV 111.2 ± 27,7. A média de concentração sérica de testosterona foi de 0,89 ± 0,52 ng/dl. Altas correlações foram observadas entre as características de biometria testicular e volume testicular. Baixas a médias correlações entre as características testiculares com produção espermática e com concentração de testosterona foram observadas. O tamanho testicular pode ser uma importante característica que auxilia na predição do potencial reprodutivo do garanhão pôneis da raça Brasileira. Sugere-se que não há variações na concentração sérica de testosterona nos pôneis da raça Brasileira que poderia afetar em sua fertilidade.

Biometria testicular e características morfológicas dos espermatozoides de cachorros-do-mato Cerdocyon thous Linnaeus, 1766 (Carnivora, Canidae) / Testicular biometry and morphological characteristics of crab-eating foxsperm Cerdocyon thous Linnaeus, 1766 (Carnivora, Canidae)

A pesquisa teve por objetivos avaliar a biometria testicular, coletar o sêmen e analisar as características morfológicas dos espermatozoides de cachorros-do-mato Cerdocyon thous Linnaeus, 1766. Foram utilizados cinco animais, maiores de três anos de idade e com peso superior a 6 kg. Os animais foram anestesiados com xilazina 2%, cloridrato de ketamina 10% e atropina 1%. Foi determinado o volume testicular (testículos medidos com paquímetro). O sêmen foi coletado pelo método de eletroejaculação e a morfologia espermática analisada pelo método de preparação em câmara úmida, sendo realizado também o teste supravital (corante eosina/ nigrosina). O volume médio dos testículos foi 2,96 ± 0,56 cm3. A análise da morfologia espermática teve média de 54,2 % de defeitos no formato da cabeça. No teste supravital obteve-se 77,4 ± 5,17 % de células vivas.  Conclui-se que os cachorros-do-mato (Cerdocyon thous) possuem volume testicular menor que outros canídeos selvagens; que a técnica de coleta de sêmen foi eficiente para a espécie e que o sêmen apresenta elevada patologia espermática, talvez devido à pequena variabilidade genética da população.(AU)

This research aimed to evaluate the testicular biometry, collect semen and analyze the morphological characteristics of the spermof crab-eating fox Cerdocyon thous Linnaeus, 1766. Five animals older than three years-old and weighing more than 6 kg wereused. The animals were anaesthetized with 2% of xylazine, 10% of ketamine hydrochloride, and 1% of atropine. The testicularvolume was determined (testicles measured with caliper ruler). The semen was collected by the electroejaculation method, andthe spermatozoa morphology was analyzed by the preparation in a humidified chamber method. The supravitally test was alsoperformed (eosin /nigrosine pigment). The average volume of the testicles was 2,96 ± 0,56 cm3. Analysis of the morphology hada mean of 54.2% of total defects and high incidence of bent and rolled tails, cytoplasmic droplets and head shape defects. Insupravitally tests were obtained 77.4 ± 5.17% of alive cells. We conclude that the crab-eating foxes (Cerdocyon thous) have lowertesticular volume than other wild canids, that the semen collection technique was efficient for the species, and that the semen hashigh sperm pathology, perhaps due to the low genetic variability of the population.(AU)

Effect of racial crossing on the scrotum-testicular biometry in sheep submitted to scrotal insulation / Efeito do cruzamento racial sobre a biometria escroto-testicular em ovinos submetidos à insulação escrotal

The objective of this study was to evaluate the effect of the racial crossing on scrotum-testicular biometric characteristics of four sheep Santa Ines and four crossbred (Santa Ines x Dorper) submitted to scrotal insulation, following the return of these characteristics to values previously reported. For this, two measurements were made regarding the scrotal circumference (SC), length (L) and testicular width (W) before treatments. The testicular volume (V) was calculated by the formula V=2 [(r2) x π x H]. The pouches were made with double-layer plastic, internally lined with cotton, and fixed around the spermatic funiculus and scrotum with adhesive tape and bandage remaining in this position for seven days. Measurements were made every seven days, totaling 15 measurements throughout the experiment. The data were submitted to analysis of variance (ANOVA) to a randomized block design with two blocks, 15 treatments and four replications. The variables analyzed were subjected to Dunnett test at 5% probability, to compare the values obtained before and after insulation. For comparison between the breeds, the variables were submitted to Tukey test at 5% probability. All animals studied suffered significant influence (P<0.05) after scrotal insulation, but Santa Ines returned to the values previously observed in a shorter period than the crossbred. In summary, sheep Santa Ines have a higher resistance than crossbred animals when subjected to thermal stress induced by scrotal insulation.(AU)

Objetivou-se neste trabalho avaliar o efeito do cruzamento sobre as características biométricas escroto-testiculares de carneiros da raça Santa Inês e mestiços (Santa Inês x Dorper), submetidos à insulação escrotal, acompanhando o retorno dessas características aos valores previamente observados. Para isso, foram realizadas duas mensurações referentes à circunferência escrotal (CE), comprimento (C) e largura testicular (L), antes da insulação. O volume testicular (V) foi calculado pela fórmula V=2 [(r2) x π x h]. As bolsas de insulação foram fixadas ao redor do funículo espermático e escroto com fita adesiva e esparadrapo, permanecendo por sete dias. Após o período de insulação, as mensurações foram realizadas a cada sete dias, totalizando 15 mensurações durante todo o experimento. Os dados obtidos foram submetidos à análise de variância (ANOVA) para um delineamento em blocos casualizados, com dois blocos, 15 tratamentos e quatro repetições. As variáveis analisadas foram submetidas ao teste de Dunnett a 5% de probabilidade, para comparar os valores obtidos antes e após a insulação. Para a comparação entre as raças, as variáveis foram submetidas ao teste de Tukey a 5% de probabilidade. Todos os animais estudados sofreram influência significativa (P<0,05) após a insulação escrotal, porém os carneiros da raça Santa Inês retornaram aos valores anteriormente observados em um espaço de tempo mais curto do que os mestiços. Conclui-se que carneiros da raça Santa Inês apresentam maior resistência do que animais mestiços quando submetidos ao estresse térmico induzido pela insulação escrotal.(AU)

Effect of racial crossing on the scrotum-testicular biometry in sheep submitted to scrotal insulation / Efeito do cruzamento racial sobre a biometria escroto-testicular em ovinos submetidos à insulação escrotal

The objective of this study was to evaluate the effect of the racial crossing on scrotum-testicular biometric characteristics of four sheep Santa Ines and four crossbred (Santa Ines x Dorper) submitted to scrotal insulation, following the return of these characteristics to values previously reported. For this, two measurements were made regarding the scrotal circumference (SC), length (L) and testicular width (W) before treatments. The testicular volume (V) was calculated by the formula V=2 [(r2) x π x H]. The pouches were made with double-layer plastic, internally lined with cotton, and fixed around the spermatic funiculus and scrotum with adhesive tape and bandage remaining in this position for seven days. Measurements were made every seven days, totaling 15 measurements throughout the experiment. The data were submitted to analysis of variance (ANOVA) to a randomized block design with two blocks, 15 treatments and four replications. The variables analyzed were subjected to Dunnett test at 5% probability, to compare the values obtained before and after insulation. For comparison between the breeds, the variables were submitted to Tukey test at 5% probability. All animals studied suffered significant influence (P<0.05) after scrotal insulation, but Santa Ines returned to the values previously observed in a shorter period than the crossbred. In summary, sheep Santa Ines have a higher resistance than crossbred animals when subjected to thermal stress induced by scrotal insulation.(AU)

Objetivou-se neste trabalho avaliar o efeito do cruzamento sobre as características biométricas escroto-testiculares de carneiros da raça Santa Inês e mestiços (Santa Inês x Dorper), submetidos à insulação escrotal, acompanhando o retorno dessas características aos valores previamente observados. Para isso, foram realizadas duas mensurações referentes à circunferência escrotal (CE), comprimento (C) e largura testicular (L), antes da insulação. O volume testicular (V) foi calculado pela fórmula V=2 [(r2) x π x h]. As bolsas de insulação foram fixadas ao redor do funículo espermático e escroto com fita adesiva e esparadrapo, permanecendo por sete dias. Após o período de insulação, as mensurações foram realizadas a cada sete dias, totalizando 15 mensurações durante todo o experimento. Os dados obtidos foram submetidos à análise de variância (ANOVA) para um delineamento em blocos casualizados, com dois blocos, 15 tratamentos e quatro repetições. As variáveis analisadas foram submetidas ao teste de Dunnett a 5% de probabilidade, para comparar os valores obtidos antes e após a insulação. Para a comparação entre as raças, as variáveis foram submetidas ao teste de Tukey a 5% de probabilidade. Todos os animais estudados sofreram influência significativa (P<0,05) após a insulação escrotal, porém os carneiros da raça Santa Inês retornaram aos valores anteriormente observados em um espaço de tempo mais curto do que os mestiços. Conclui-se que carneiros da raça Santa Inês apresentam maior resistência do que animais mestiços quando submetidos ao estresse térmico induzido pela insulação escrotal.(AU)