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This study aimed to evaluate the nutrient intake, milk production, and production costs of dairy goats fed diets containing the rations with cactus cladodes (Opuntia strictaor Nopalea cochenillifera) associated with silage (Sorghum bicolor)or Tifton hay (Cynodonspp).Twelvepluriparous Saanen and Anglo Nubiana goats (42.25 ± 5.48kg) at eight weeks of lactation were used. Treatments lasted 60 days, consisting of four periods of 15 days, distributed in three Latin square (4×4) with four diets. The treatments represented by diets with differentassociatedfeed: SSOP (Sorghum Silage + Opuntia); THOP (Tifton hay + Opuntia); SSNO (Sorghum Silage + Nopalea); and THNO (Tifton hay + Nopalea), in addition to concentrate in all diets. There were differences (p <0.05) for the intakes of DM, OM, with higher values observed for goats fed the diets THNO, THOP, and SSNO, and for CP, NDF and ADF were higher for THNO and SSNO diets. Similar behavioroccurred for voluntary and total water intake. The milk production and feed efficiency did not differ(p >0.05). Costs with food, milk revenue, and gross revenue were not influenced by diets(p >0.05).The Opuntiaor Nopaleacactus cladodes genotypes associated with sorghum silage or tifton hay can be used to feed dairy goats in the semiarid region.(AU)

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As a positional and geometrical isomer of linoleic acid, trans 10, cis 12 conjugated linoleic acid (t10c12-CLA) reduces white fat by reducing food intake, modulating lipid metabolism, and stimulating energy expenditure. However, the t10c12-CLA products are mostly mixtures, making it difficult to obtain accurate results. Studies are needed to investigate the effects of pure t10c12-CLA on animals and humans. In this study, we used the biallelic transgenic (tg) mice, which could produce t10c12-CLA itself, to investigate the effects of pure t10c12-CLA on female reproductive ability. The results showed that the body and relative ovary weights had no significant difference between tg and wild-type (wt) littermates at ages 3 or 10 weeks. While the fecundity test found that tg mice had a significantly longer first litter time (32.0 ± 4.70 days vs. 21.3 ± 2.31 days, P<0.05), and a significantly lower number of litters (4.75 ± 2.75 vs. 6.67 ± 0.57, P<0.05) when compared with wt mice during continuous mating within seven months. Hormone profiles showed that serum estradiol levels did not change in tg mice; however, significantly (P<0.05) decreased progesterone and increased prostaglandin E2 levels were observed in tg mice compared with those of wt mice. Hematoxylin-eosin staining showed no pathological characteristics in tg ovaries, except for the increased atresia follicles (P<0.05). Moreover, the tg mice had a significantly more extended diestrus period than the wt mice (48.4 ± 6.38% vs. 39.6 ± 3.81%, P<0.05). In summary, t10c12-CLA could affect serum progesterone and prostaglandin E2 levels, lead to a disordered estrus cycle, and impact the reproductive performance of female mice. This study provided theoretical and biosafety recommendations for applying t10c12-CLA in female mammals.(AU)

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Diets composed of whole flint corn grain (WCG) without any roughage source are often used in South American countries. The primary source of protein in theses diets is soybean meal. We hypothesized that the combination of different protein sources improves ruminal fermentation and nutrient digestibility. This study was carried out to assess the impacts of replacing 50% of the soybean meal by other protein sources on voluntary dry matter intake (DMI), digestibility, efficiency of N utilization, efficiency of grain use, metabolic characteristics, and ingestive behavior of Nellore cattle fed WCG diets. Five rumen-cannulated Nellore Bulls (average BW = 651.6 ± 31.83 kg) were used in a 5 x 5 Latin square design, randomly assigned to five diets consisting of 85% of WCG and 15% of pellet supplement with combination of different protein sources: SM - pellet supplement with soybean meal only; CM - soybean meal and cottonseed meal; SFM - soybean meal and sunflower meal; DDG - soybean meal and DDG; DDGS - soybean meal and DDGS. The replacement of 50% of soybean meal by DDG increased (P<0.05) DMI and organic matter intake (OMI), both DMI and OMI were lower with CM. Digestibility, ruminal pH (mean = 5.7), efficiency of N utilization, and diurnal ingestive behavior were not affected (P>0.05) by protein sources. Protein sources also did not affect the grain-use efficiency (P>0.05), with an average value of 96.6% and only 3.4% of the corn grains recovered in the feces. The concentration of GGT enzyme was greatest (P<0.05) for CM and DDGS. The inclusion of DDG increased the DMI but did not improve the nutrients' digestibility or N metabolism. The results suggest that 50% of the soybean meal can be replaced by sunflower meal, DDG, and DDGS without affecting the nutritional and metabolic parameters of Nellore cattle fed whole flint corn grain diets, providing opportunities for reducing costs in feedlot systems.(AU)

Dietas com milho grãos inteiros (MGI) e sem volumoso são frequentemente usadas em países da América do Sul. A principal fonte de proteína nessas dietas é o farelo de soja. Nossa hipótese é que a combinação de diferentes fontes de proteína melhora o padrão de fermentação ruminal e a digestibilidade dos nutrientes. Este estudo foi realizado para avaliar os impactos da substituição de 50% do farelo de soja por outras fontes proteicas sobre o consumo voluntário, digestibilidade, eficiência de utilização do N, eficiência de utilização de grãos, características metabólicas e comportamento de bovinos Nelores alimentados com dietas de MGI. Cinco bovinos Nelores, machos não-castrados e com cânula ruminal (Peso corporal médio = 651,6 ± 31,83 kg) foram utilizados em delineamento quadrado latino 5 x 5, distribuídos aleatoriamente em cinco dietas com 85% de MGI e 15% de um suplemento peletizado com combinação de diferentes fontes proteicas, a saber: FS - núcleo somente com farelo de soja; FA - núcleo com farelo de soja e farelo de algodão; FG - núcleo com farelo de soja e farelo de girassol; DDG - núcleo com farelo de soja e DDG; DDGS - núcleo com farelo de soja e DDGS. A substituição de 50% do farelo de soja por DDG aumentou (P<0,05) o consumo de matéria seca (CMS) e o consumo de matéria orgânica (CMO). Tanto o CMS quanto o CMO foram menores com FA. A digestibilidade dos nutrientes, pH ruminal (média = 5,7), eficiência de utilização do N e comportamento não foram afetados (P>0,05) pelas fontes proteicas. A eficiência de utilização dos grãos também não modificou (P>0,05) com a combinação das fontes proteicas, com valor médio de 96,6% e cerca de apenas 3,4% dos grãos de milho recuperados nas fezes. A concentração da enzima gama aminotransferase foi maior para CM e DDGS (P>0,05). A inclusão de DDG aumentou o CMS, mas não melhorou a digestibilidade dos nutrientes da dieta ou o metabolismo do N. Os resultados sugerem que 50% do farelo de soja pode ser substituído por farelo de girassol, DDG ou DDGS sem afetar os parâmetros nutricionais e metabólicos de bovinos Nelore alimentados com dietas de milho grão inteiro, fornecendo uma oportunidade para reduzir os custos.(AU)

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This study aimed to evaluate the intake, digestibility, and ingestive behavior of sheep fed with different species of forage cacti. Fifteen sheep (17.27kg ± 1 kg) were distributed in a completely randomized experimental design with three treatments and five replicates. The treatments were diets on a dry matter basis composed of 430.9 g kg-1of thornless Mandacaru cactus (Cereus hildmannianus), 525.7 g kg-1of cactus pear cv. Orelha de Elefante Mexicana (Opuntia stricta) and 492.1 g kg-1of cactus pear cv. Miúda (Nopalea cochenillifera) in addition to Sabiá hay (Mimosa caesalpiniaefolia) (194.7 to 233.8 g kg-1), plus concentrate feed. The intake of the dry matter,organic matter, ether extract, neutral detergent fiber, total carbohydrates, non-fiber carbohydrates, total digestible nutrientsand voluntary water intake in g day-1was not differ (p > 0.05) by experimental diets. There were no differences (p > 0.05) in digestibility coefficients of the dry matter,organic matter, neutral detergent fiber, total carbohydrates, non-fiber carbohydrates, and total digestible nutrientsbetween the experimental diets. The feeding times differed (p < 0.05) between diets, with a higher value for the Opuntiadiet, while the rumination times, feeding efficiency, and rumination efficiency did not differ (p > 0.05). The cactus Cereusand Opuntiaand Nopaleahave similar nutritional value in sheep's diet.(AU)

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Copaiba oleoresin, extracted from the Copaifera reticulata tree, has been used as a remedy in popular medicine in the Brazilian Amazon for various purposes, including reducing drug abuse. Yet no studies evaluated the effect of repeated administration of copaiba oil on alcohol consumption in animals. To evaluate this effect, we divided adult male Wistar rats into a) an alcohol group in which the animals had free access to choose between two bottles: one containing alcohol solution (20%) and another containing vehicle solution (0.2% saccharin); and b) a control group with access to two bottles containing vehicle solution. Rats were free to drink 24 h per day, for 35 days. Daily alcohol consumption and weekly body weight gain and food intake were monitored. From day 22, half of the rats in each group received 600 mg kg-1 copaiba oleoresin and the other received vehicle, subcutaneously, once a day, for three days. On day 35, rats were evaluated in an open-field test. The results showed that copaiba oil decreased voluntary alcohol intake and preference between days 2 and 6 after the last administration. Copaiba treatment also decreased the food intake and body weight gain in both alcohol and control groups without changing behaviors in the open-field test. Therefore, copaiba oil was able to reduce voluntary alcohol consumption in rats and could be tested in humans as an adjuvant to treat alcohol use disorder.(AU)

O óleo extraído da árvore copaíba, Copaifera reticulata, tem sido usado na medicina popular na Amazônia brasileira para diversos fins, incluindo abuso de drogas. Contudo, não há estudos avaliando o efeito da administração repetida do óleo de copaíba sobre o consumo de álcool em animais. Para avaliar esse efeito, dividimos ratos Wistar machos adultos em dois grupos: a) um grupo álcool, no qual os animais tinham livre acesso a duas garrafas: uma contendo solução alcoólica (20%) e outra contendo solução veículo (sacarina 0,2%); e b) um grupo controle com acesso a duas garrafas contendo solução veículo. Os ratos podiam beber livremente, 24 horas por dia, durante 35 dias. O consumo diário de álcool, bem como o ganho de peso corporal semanal e a ingestão de alimentos foram monitorados. A partir do dia 22, metade dos ratos de cada grupo recebeu 600 mg kg-1 de óleo de copaíba e a outra metade recebeu veículo, por via subcutânea, uma vez ao dia, durante três dias. No dia 35, os ratos foram testados em teste de campo aberto. Os resultados mostraram que o óleo de copaíba diminuiu a ingestão voluntária e a preferência por álcool entre os dias 2 e 6 após a última administração. O tratamento com óleo de copaíba também diminuiu a ingestão alimentar e o ganho de peso corporal em ambos os grupos álcool e controle, sem alterar o comportamento no teste de campo aberto. Portanto, o óleo de copaíba foi capaz de reduzir o consumo voluntário de álcool em ratos e poderia ser testado em humanos como um adjuvante para tratar transtorno de uso de álcool.(AU)

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The cashew pseudo fruit can be used to animal feeding in tropical and subtropical countries as Brazil, Ivory Coast and Vietnam. Thus, our objective was to evaluate the intake, digestibility of nutrients and nitrogen balance of dehydrated cashew apple by-product to sheep. The experiment was carried out with 24 sheep in a completely randomized design with the treatments distributed in a 4 x2 factorial scheme to test the inclusion (11, 21, 28 and 33% dry matter basis) and the grinding sizes (3 and 19 mm diameter) of dehydrated cashewappleby-product. To grinding sizes there was no effect to intake and digestibility, suggesting the use of dehydrated cashewappleby-product either finely or coarsely milled. The inclusion up to 33% of dehydrated cashewappleby-product inclusion did not affect voluntary intake and nitrogen balance. However, when including above 21%, there was a reduction of ether extract digestibility and more than 28% reduced dry matter and organic matter digestibility.(AU)

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This study was conducted with the aim of evaluating the effect of protein-energy supplementation on productive performance, intake, digestibility, and microbial protein synthesis in heifers fed tropical forage. Twelve Nellore heifers at 11 months of age, with a mean initial body weight of 248.8 ± 11.40 kg were used. The animals were distributed in a completely randomized design with two treatments and six replicates. The treatments evaluated were: control (without supplementation); and supplementation in the amount of 1.5 kg animal-1 day-1. Forage samples were collected to evaluate the chemical composition and the total availability of dry matter of the pasture consumed by the animals. To evaluate the intake and forage digestibility, a trial was performed using internal and external markers. In this study no effect of supplementation was observed on the intakes of dry matter (DM), organic matter (OM), forage DM, or neutral detergent fiber corrected for ash and protein (NDFap; P > 0.10). However, supplementation increased (P < 0.01) the intakes of crude protein (CP), ether extract (EE), and non-fibrous carbohydrates (NFC). Additionally, an upward trend (P=0.076) was observed in the intake of digested OM (DOM) with supplementation. On the other hand, supplementation increased (P < 0.01) the digestibility coefficients of OM, CP, EE, and NFC, although supplementation did not affect the ruminal synthesis of nitrogen compounds (MICN), efficiency of protein microbial synthesis (EMS), relative microbial nitrogen (RMICN) or urine urea nitrogen (UUN) excretion (P > 0.57). Lastly, supplementation increased (P=0.050) the average daily gain of the animals, although no effect (P > 0.10) was observed on their final body weight. These results suggested that protein-energy supplementation improves the productive performance and nutritional characteristics of grazing beef heifers.(AU)

Este estudo foi conduzido com o objetivo de avaliar o efeito da suplementação proteico-energético sobre o desempenho produtivo, consumo, digestibilidade e sínteses de proteína microbiana em novilhas de corte alimentadas com forragem tropical. Doze novilhas Nelore com 11 meses de idade e peso corporal médio inicial de 248,8±11,40 kg foram utilizadas. Os animais foram distribuídos em delineamento inteiramente casualizado com dois tratamentos e seis repetições. Os tratamentos avaliados foram: controle (não suplementado); e suplementado na quantidade de 1,5 kg animal-1 dia-1. Amostras de forragem foram coletadas para avaliar a composição química e a disponibilidade total de matéria seca do pasto consumido pelos animais. Para avaliar o consumo e digestibilidade da forragem, um ensaio foi conduzido usando marcadores internos e externos. Neste estudo não foi observado efeito da suplementação sobre o consumo de matéria seca (MS), matéria orgânica (MO), MS da forragem, ou fibra em detergente neutro corrigida para cinzas e proteína (FDNcp; P > 0,10). No entanto, a suplementação incrementou o consumo de proteína bruta (PB), extrato etéreo (EE) e carboidratos não fibrosos (CNF). Além disso, foi observada uma tendência de aumento (P=0,076) no consumo de MO digerida (MOD) com a suplementação. Por outro lado, a suplementação aumentou (P < 0,01) os coeficientes de digestibilidade da MO, PB, EE e CNF, embora a suplementação não tenha afetado a síntese ruminal de compostos nitrogenados (NMIC), eficiência de síntese de proteína microbiana (EFM), nitrogênio microbiano relativo (NMICR) ou excreção de nitrogênio ureico na urina (NUU) (P > 0,57). Finalmente, a suplementação aumentou (P < 0,050) o ganho médio diário dos animais, embora nenhum efeito (P > 0,10) tenha sido observado no peso corporal final. Esses resultados sugerem que a suplementação proteico-energética melhora o desempenho produtivo e as características nutricionais de novilhas de corte em pastejo.(AU)

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Meteorological variables are the main abiotic factors that directly influence reactions in rodent metabolism, activating the action of thermoregulatory mechanisms aimed at homeostasis and can involve several factors that work together to dissipate or conserve body heat. In this scenario, we aimed to address, synthetically, the main means that rodents have to adapt and survive faced with the thermoregulatory challenges posed by the environment in which they live. Among these processes, behavioral changes can occur in water and food intake, as well as voluntary displacement to environments offering greater thermal comfort. The autonomic processes involve innate mechanisms that act quickly to facilitate thermal exchange, such as sweating, respiratory evaporation, and changes in peripheral blood flow. Adaptation involves evolutionary processes that, in the long run, alter the morphophysiological characteristics of the epidermis and coat, in order to conserve or dissipate heat. Knowledge of the role of these processes in adaptive physiology is essential to understand how the thermal environment affects rodents and how deleterious effects are mitigated.

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The objective with this study was to estimate the voluntary feed intake (VFI) of sows during the occurrence of heat waves in the Triângulo Mineiro and Alto Paranaíba mesoregion, Minas Gerais, Brazil. The Instituto Nacional de Meteorologia (INMET) database was used to calculate the temperature-humidity index (THI) from 2008 to 2017 for Araxá, Frutal, Ituiutaba, Patos de Minas, Patrocínio, Uberaba, and Uberlândia, and the estimated VFI with and without effective occurrence of heat waves (EOH). An economic estimation of the revenue based on the VFI was performed. The data were analyzed by Friedman's non-parametric test (two-way non parametric analysis), using the significance level of 5%. The sows raised in Patrocínio showed a higher average prediction of VFI (6.611 kg without EOH and 5.743 kg with EOH) than Ituiutaba (6.424 kg without EOH and 3.969 kg with EOH) and Frutal (5.868 kg without EOH and 4.648 kg with EOH). In the classification "without EOH", the microregions of Araxá (6.502 kg), Uberaba (6.452 kg), and Uberlândia (6.478 kg) did not differ from the other cities, but "with EOH", Araxá (4.864 kg) differed from Patrocínio (5.743 kg). The reduction of VFI due to thermal stress represented a revenue reduction of R$ 74.60 per litter. The averages for predicted VFI were lower in the EOH category than in the probable occurrences of heat waves category for all cities. The predominance of heat waves reduces the VFI of sows, particularly in Ituiutaba and Frutal.(AU)

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This work examines the economic advantages of probiotic use in the diet of Nile tilapia broodstock during the reproductive period. For this purpose, Bacillus subtilis was applied as a feed additive. The experimental design was completely randomized with three treatment groups: the T0 control (without probiotic), the T1 continuous probiotic intake, and the T2 alternate probiotic intake at a dose of 0.50 g kg-1 of feed (1010 CFU g-1 ) with four replicates. For the reproduction assay, 118 females and 48 males of Nile tilapia (proportion 4 males:9 females. hapa-1 ) (weight 527.65 g ± 185.98 g and length 30.16 cm ± 3.57 cm) were distributed into 12 hapas (3.5 × 2.0 × 1.5 m). Reproductive variables (spawning female percentage, egg production, and fry production) were used to calculate the economic feasibility indexes (total cost of nutrition [TcN], gross revenue [GR], and total operational profit [ToP]). The results show increasing values for spawning female number, collected eggs, and surviving fry in the probiotic groups. We recommend continuous intake of probiotic (feed with addition of probiotic) at a dose of 0.5 g kg-1 of feed (1010 CFU g-1 ) during the breeding season of Nile tilapia, due to the suitable reproductive indexes and profitability.

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Voluntary consumption (VolC) and ingestive behaviour (IB) of lambs fed diets containing different percentages (0.0; 7.5; 15.0 and 22.5%) of babassu mesocarp bran (I-BMB) were evaluated. The trial was at the Federal University of Tocantins, Campus de Araguaína-TO. Twenty lambs were evaluated using a completely randomized design, and five replications were performed. VolC data was obtainedby measuring total volumes of both food supplied and food remaining after feeding. IB was evaluated throughout two, non-consecutive 24-h periods, with observations were recorded every five minutes that described the following behavioural variables: resting (REST), rumination (RUM), intake (INT) and chewing (CHEW). The particle size was also measured using a Penn State particle separator. All VolC parameters (dry matter intake, DMI; crude protein intake, CPI; neutral detergent fibre intake, NDFI; and acid detergent fibre intake, ADFI) increased linearly with I-BMB intake. I-BMB consumption did not interfere with water intake, nor did it affect I-BMB did not affect the duration of DMI, NDFI and ADFI. However, linear increases of rumination and chewing activities associated with DMI, NDFI and ADFI were observed as I-BMB levels increased. In summary, effects of inclusion I-BMB in diets of lambs included increased voluntary consumption, and reduced rumination and chewing, but not ingestion, efficiency. When economically favorable, these data suggest that inclusion of up to 22.5% I-BMB within balanced diets should be applied.

Avaliou-se o consumo voluntário (CVol) e o comportamento ingestivo (CI) de borregas alimentadas com rações contendo diferentes inclusões de farelo do mesocarpo do babaçu (I-FMB) (0,0; 7,5; 15,0 e 22,5%). O local do ensaio foi a ovinocaprinocultura da Universidade Federal do Tocantins, Campus de Araguaína-TO. Foram utilizadas 20 borregas distribuídas em delineamento inteiramente casualisado, com cinco repetições. O CVol foi realizado pela mensuração do total de alimento fornecido e das sobras. Já o CI foi avaliado em dois períodos de 24 horas em dias não consecutivos, com observações de cinco em cinco minutos sendo as variáveis comportamentais: ócio (O), ruminação (R), ingestão (I)e mastigação (M). Mensurou-se também o tamanho de partícula com separador de partículas modelo Penn State. Foi observado aumento linear crescente com a I-FMB para todos os parâmetros de CVol estudados (consumo de matéria seca - CMS, consumo de proteína bruta - CPB, consumo de fibra em detergente neutro - CFND e consumo de fibra em detergente ácido - CFDA) em todas as unidades (gdia-1 animal-1, % do peso vivo (PV), g kg-0,75). A I-FMB não interferiu na ingestão de água de borregas. A I-FMB não afetou o tempo em ingestão por CMS, CFDN e CFDA. No entanto, a I-FMB aumentou linearmente as atividades ruminação e mastigação em função do CMS, CFDN e CFDA. A I-FMB na dieta de borregas aumenta o consumo voluntário de ovinos e influência de forma negativa na eficiência de ruminação e mastigação, sem prejudicar eficiência de ingestão. Quando economicamente favorável recomenda-se a inclusão de até 22,5 % de FMB em dietas balanceadas.

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This research was conducted to investigate the effect of live and inactive sugarcane yeast on beef cattle voluntary intake, apparent digestibility of nutrients, ruminal pH, volatile fatty acids (VFA) concentrations, and ruminal ammonia. Five rumen-cannulated Nellore heifers were distributed in a 5×5 Latin square design, with five experimental periods of 15 days, with seven days for adaptation to the additives and the remaining days for data records and sampling. Total mixed ration (TMR) was composed by corn silage (20%) and concentrate (80%) based on corn and soybean meal. Five treatments were evaluated: without additive use (negative control; NC); chemical buffer addition ­ 0.71% in concentrate DM of sodium bicarbonate and 0.18% of magnesium oxide (positive control, PC); 10 g/day live yeast (LY); 15 g/day of inactive yeast (IY15); and 30 g/day of inactive yeast (IY30). Sugarcane yeasts were directly infused in rumen immediately after morning and afternoon feed supply. Feed additives did not affect voluntary intake, nutrient digestibility, and sorting behavior of animals. However, heifers from all treatments presented preferential intake of fibrous fraction of diet, especially those from NC and IY15. Nitrogen balance, VFA concentrations, and blood parameters were not influenced as well. Sodium bicarbonate and magnesium oxide led to greater ruminal pH than yeast, and IY15 presented greater pH than IY30. Ruminal ammonia was increased by the use of additives. Active and inactive yeasts are not recommended as feed additives for bovines fed diets with 80% of concentrate since it allows animals to select fibrous particles from TMR, and no representative gain in ruminal parameters and digestibilities are guaranteed.(AU)

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Objetivou-se avaliar os fatores da composição da dieta que podem interferir no consumo, na digestibilidade, na produção e na composição do leite de vacas mestiças Holandês x Zebu confinadas em condições experimentais no Brasil. A metanálise foi utilizada para o levantamento de trabalhos científicos de domínio público, compreendendo o período de janeiro de 2000 a dezembro de 2015. A base de dados foi constituída por 40 experimentos com 159 tratamentos, envolvendo 735 vacas em lactação. O consumo de nutrientes digestíveis totais (NDT) é comprometido pelo teor de fibra em detergente ácido (FDA) da dieta, relacionando-se com a digestibilidade da matéria seca (MS) e da fibra em detergente neutro (FDN), o que repercute na eficiência alimentar (EA). Observa-se a relação do teor de carboidratos não fibrosos (CNF) da dieta com o teor de lactose e o efeito do teor de MS da dieta e o elevado teor de FDN no consumo em função das características específicas dos tipos de volumosos utilizados. O consumo de NDT da dieta é prejudicado pela digestibilidade da MS e, principalmente, da FDN em função da proporção e das características dos volumosos utilizados na dieta, e esses fatores repercutem na EA, bem como na produção e na composição do leite.(AU)

The aim was to evaluate diet composition factors that may interfere with intake, digestibility, milk yield and composition of crossbred Holstein x Zebus cows confined under Brazilian experimental conditions. The meta-analysis was used for the survey of scientific works of public domain, covering the period from January 2000 to December 2015. The database consisted of 40 experiments with 159 treatments, involving 735 lactating cows. The voluntary intake of total digestible nutrients (TDN) is compromised by the acid detergent fiber (FAD) content of the diet, related to dry matter digestibility (DM) and neutral detergent fiber (NDF), affecting dietary efficiency (DE). The relation of the non-fibrous carbohydrate (NFC) content of the diet with the lactose content and the effect of the DM diet content and the high NDF content on the voluntary intake are specific characteristics of the types of roughage used. Dietary NDT intake is affected by the digestibility of DM and, mainly, NDF, depending on the proportion and characteristics of the nutrients used in the diet, and these factors have an impact on dietary efficiency, as well as on milk composition.(AU)

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Corn ensiling is a feed preservation practice used for ruminant production. During the process of making and opening the silos, the quality of the silage may change due to the entrance of air, which can cause damage and decrease the voluntary intake of the animals. The objective of this work was to evaluate the chemical composition of corn silages during their use in commercial bunker silos, as well as to correlate this with qualitative and quantitative characteristics related to the silage making process. Technical data on silage making and samples were collected from 14 bunker silos, with five samples collected along each silo (at 10, 30, 50, 70 and 90% of their lengths); additionally, two sampling methodologies were compared (“W” vs. layer collections in silo panels: top, middle and bottom). The values for the pH and titratable acidity (TA) were significant (p 0.05). According to the collected technical data, 78.57% of the bunker silos were built directly on the ground, the harvesting equipment was mostly borrowed from municipalities or farmers’ associations (for 35.7 and 28.5% of the farms, respectively), and in only 64.2% of the farms, the harvester was sharpened before silage cutting. Good practices were applied during the corn silage process and throughout the use of the silos, homogeneous quality was detected in the western region of Santa Catarina. Either “W” or layer collections can be used to sample corn silage.

A ensilagem de milho é uma prática de conservação de alimento utilizada na produção de ruminantes. Durante o processo de confecção e abertura do silo, a silagem pode ter alterações em sua qualidade em função da entrada de ar, que pode causar prejuízos e diminuir o consumo dos animais. Objetivou-se avaliar a composição bromatológica das silagens de milho ao longo da utilização, de silos comerciais do tipo trincheira, bem como correlaciona-las com as características qualitativas e quantitativas, relacionadas ao processo de confecção das silagens. Os dados técnicos sobre a confecção da silagem, bem como amostras foram coletas à campo em 14 propriedades, em que foram coletadas cinco amostras ao longo de cada silo (relativos à 10, 30, 50, 70 e 90% do seu comprimento), e adicionalmente duas metodologias de amostragem foram comparadas (coletas em “W” vs. coletas em estratos do painel do silo: topo, meio e base). Os valores de pH e AT (p 0.05). O levantamento técnico mostrou que 78,57% dos silos trincheira avaliados foram construídos diretamente no solo, que os equipamentos de colheita utilizados foram emprestados de prefeituras ou associações de agricultores (para 35,7% e 28,5% das fazendas), ou que em somente 64,2% das fazendas o equipamento de colheita foi afiado antes da colheita. Foram observadas boas práticas de ensilagem de milho e homogênea qualidade ao longo do silo. Ambos métodos de coleta, em “W” como em estratos do painel podem ser usados durante amostragem de silagem de milho.

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Dry matter and neutral detergent fiber contents act as physical regulators of voluntary intake in ruminants. Therefore, different levels of these components may interfere with animal performance and require careful assessment of the used raw material. This study was conducted with the purpose to assess the effect of soybean hulls and white oat in distinct or associated uses in the concentrate diet on the ingestive behavior of cattle. Thirty-six steers with an initial mean age of 20 months and initial mean weight of 226 kg were used. The diet was composed of 50% sorghum silage and 50% concentrate based on dry matter. The tested diets were soybean hulls (concentrate fraction composed mainly of soybean hulls), white oat grains (concentrate fraction composed mainly of white oat grains), and mixture (concentrate fraction composed of equal parts of soybean hulls and white oat grains). The experimental design was a randomized block design with 12 animals per diet. The data were compared by the PROC MIXED procedure with repeated measures in time, and the means were compared by the least significant difference test at 5% significance level. Diets presented different contents of neutral detergent fiber (66, 56, and 47%) and lignin (3.2, 3.7, and 4.1%) as the percentage of white oat grain increased in the diet. The total rumination time was lower (447 minutes) for animals that received equal parts of soybean hulls and white oat grains in the diet when compared to animals receiving a diet containing soybean hulls (483 minutes) and white oat grains (495 minutes), with no difference from each other.[...]

Os teores de matéria seca e fibra em detergente neutro atuam como reguladores físicos do consumo voluntário em ruminantes. Assim, diferentes níveis destes componentes podem interferir no desempenho animal e necessitam de avaliação criteriosa da matéria-prima utilizada. O estudo foi conduzido com o objetivo de avaliar o efeito da casca do grão de soja e a aveia branca, em usos distintos ou associados na dieta concentrada no comportamento ingestivo de bovinos. Utilizou-se 36 novilhos com idade média inicial de 20 meses e peso médio inicial de 226 kg. A dieta foi composta de 50 % de silagem de sorgo e 50 % de concentrado com base na matéria seca. As dietas testadas foram: casca do grão de soja (fração concentrada composta principalmente por casca do grão de soja), grão de aveia branca (fração concentrada composta principalmente por grão de aveia branca) e mistura (fração concentrada composta por partes iguais de casca do grão de soja e grão de aveia branca). O delineamento experimental foi blocos ao acaso com 12 animais por dieta. Os dados foram comparados pelo procedimento PROC MIXED com avaliações repetidas no tempo e as médias, comparadas pelo teste de diferença mínima significativa ao nível de 5 % de significância. As dietas apresentaram diferentes teores de fibra em detergente neutro (66, 56 e 47 %) e lignina (3,2; 3,7 e 4,1 %) conforme aumento da participação do grão de aveia branca na dieta. O tempo de ruminação total foi inferior (447 minutos) para animais que receberam partes iguais de casca do grão de soja e grão de aveia branca na dieta em relação a animais recebendo dieta contendo casca do grão de soja (483 minutos) e grão de aveia branca (495 minutos), que não deferiram entre si.[...]

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The objective of this study was to assess the effects of an electrolyte and energy repository on the hematologic and biochemical variables of horses after a polo game and compare them to the effects of a commercial electrolyte repository and water. Twelve healthy horses, aged 5 to 10 years, mean body weight 356.25 ± 25.6 kg and in training for polo games, were randomly divided into three groups of four animals each and received one of three treatments. Animals participated in a 7-minute polo game. At the end of the match, they were taken to individual stalls where they received treatments for spontaneous hydration for 6 hours. Treatments were Hydroelectrolytic and Energy Repository (RHE) containing sodium, chloride, potassium, calcium, magnesium, dextrose, maltodextrin and, sucrose; and Commercial Paste (PCO) containing calcium, fructooligosaccharides, glycine, magnesium, potassium, sodium, and ad libitum water. The control group (Water) received water. Blood samples were collected as follows: just before the beginning of exercise (T0), immediately after exercise (T1), 3 hours after the end of exercise (T3), and 6 hours after the end of exercise (T6). The volume of voluntary intake was measured at T3 and T6. Packed cell volume and serum concentrations of sodium, potassium, chloride, calcium, magnesium, phosphorus, creatinine, total protein, plasma glucose, and lactate were...

O presente estudo objetivou avaliar os efeitos de um repositor hidroeletrolítico e energético oferecido a equinos que participaram de jogo de polo sobre as variáveis hematológicas e bioquímicas desses animais, além de compará-los aos efeitos de um repositor eletrolítico comercial e água. Doze equinos hígidos, com idade entre 5 e 10 anos, peso corporal médio 356,25 ± 25,6 kg e em treinamento para jogos de polo foram aleatoriamente divididos em três grupos, com quatro animais cada, e submetidos a um dos três tratamentos. Os animais foram submetidos a sete minutos do jogo de polo. Ao término, foram levados às baias individuais onde receberam os respectivos tratamentos para hidratação espontânea durante seis horas. Os tratamentos foram: grupo Repositor Hidroeletrolítico e Energético (RHE) composto por sódio, cloreto, potássio, cálcio, magnésio, dextrose, maltodextrina e sacarose; grupo Pasta Comercial (PCO) contendo cálcio, fruto-oligossacarídeos, glicina, magnésio, potássio e sódio, e água ad libitum; grupo controle (Água). As amostras de sangue foram coletadas nos seguintes tempos: imediatamente antes do início do exercício (T0); imediatamente após o exercício (T1); três horas após o término do exercício (T3); e seis horas após o término do exercício (T6). O volume de ingestão voluntário foi mensurado nos tempos T3 e T6. O volume globular e as concentrações séricas de sódio...

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Objetivou-se estudar o efeito da alimentação caseira, da ração comercial a granel e da ração comercial fechada para cães adultos sobre análises clínicas e aspectos comportamentais de ingestão das dietas. O delineamento experimental foi inteiramente ao acaso, com três tratamentos, sendo o tratamento 1 constituído de dieta caseira, o tratamento 2 por ração a granel tipo premium, e o tratamento 3 por ração comercial fechada do tipo premium. Foram utilizados três cães em cada um dos tratamentos, totalizando nove animais. As variáveis analisadas foram: teste de glicemia, odor fecal, escore fecal, volume fecal, análise química da urina (pH urinário, presença de proteína, sangue, corpos cetônicos, bilirrubina e glicose), avaliação visual do pelo e consumo voluntário da dieta. Os cães submetidos à dieta caseira apresentaram menor resultado de glicemia sanguínea (83,90). A urina apresentou pH mais alcalino para cães que receberam a ração a granel (8,06). Cães da dieta caseira apresentaram o mais alto escore para a qualidade do pelo. Houve um maior volume das fezes de cães que receberam a dieta de ração a granel. Cães que receberam a dieta de alimentação caseira ou a ração comercial fechada apresentaram um consumo total do alimento em menos de 10 minutos. A maioria dos resultados foi semelhante entre a dieta caseira e a ração fechada do tipo premium. A ração a granel apresentou os piores resultados.(AU)

The objective of this study was to study the effect of homemade feed, commercial feed in bulk, and closed commercial feed for adult dogs on clinical analysis and behavioral aspects of diet intake. The experimental design was completely randomized with three treatments: homemade diet, bulk premium type feed, and closed commercial feed of the premium type. Three dogs were observed in each of the treatments, totaling nine animals. The variables analyzed were: blood glycemia, fecal odor, fecal score, fecal volume, chemical urine analysis (urinary pH, presence of protein, blood, ketone bodies, bilirubin and glucose), visual evaluation of hair and voluntary dietary intake. Dogs submitted to a home diet showed lower blood glucose results (83.90). Urine was more alkaline pH for dogs that received the bulk feed (8.06). Dogs from the homemade feed presented the highest score for hair quality. There was a greater volume of feces from dogs that received the diet of bulk feed. Dogs that received the diet of home feed or closed commercial feed showed a total consumption of food in less than ten minutes. Most of the results were similar between the homemade feed and the closed-type premium diet. The dogs bulk feed presented the worst results.(AU)

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O presente trabalho teve por objetivo avaliar a influência do tipo de parto sobre a transferência de imunidade passiva e de alguns constituintes séricos de cordeiros recém-nascidos, alimentados naturalmente com colostro materno, criados no semiárido paraibano em sistema extensivo. Foram utilizados 34 cordeiros clinicamente sadios, da raça Santa Inês, os quais foram identificados e pesados imediatamente após o nascimento e separados em dois grupos experimentais com 17 animais cada. O grupo PS (nove machos e oito fêmeas) formado por animais nascidos de partos simples e o grupo PG (seis machos e onze fêmeas) formado por cordeiros nascidos de partos gemelares. A ingestão de colostro se deu de forma natural e voluntária em suas respectivas mães. Foram coletados 10 mL de sangue de cada animal, mediante punção da veia jugular, em tubos siliconizados a vácuo, 48 horas após o nascimento. Após centrifugação, as alíquotas de soro foram separadas e permaneceram congeladas a -15°C até o momento das análises. Para o estudo comparativo dos constituintes séricos, foram constituídos dois grupos experimentais distribuídos em um delineamento inteiramente casualizado, no esquema fatorial 2x2 (tipo de parto e sexo). Os dados obtidos foram submetidos à análise de variância, cujas médias foram comparadas pelo teste de Tukey a 5%. Foram determinadas as atividades séricas das enzimas aspartato aminotransferase (AST) e gamaglutamiltransferase (GGT) e as concentrações séricas de proteína total, albumina, ureia, creatinina, cálcio, fósforo e magnésio, utilizando-se conjuntos de reagentes comerciais e as leituras das amostras em espectrofotômetro automático. As atividades séricas de AST, GGT e as concentrações séricas de proteína total, albumina e globulinas dos cordeiros dos grupos PS e PG não foram influenciadas pelo tipo de gestação e sexo. A partir da concentração sérica de proteína total, verificou-se falha de transferência de imunidade passiva (FTIP) nos cordeiros do grupo PG, utilizando-se o valor 5,0g/dL como ponto de corte. Com exceção do cálcio, as concentrações séricas da ureia, creatinina, fósforo e magnésio apresentaram o mesmo padrão de comportamento. Embora esses constituintes não tenham apresentado diferença significativa entre os grupos estudados e o sexo, pôde-se observar valores mais elevados nos animais nascidos de partos simples, sugerindo que a ausência de concorrência pela ingestão voluntária de colostro materno pode ter sido o fator determinante. Pode-se concluir que cordeiros Santa Inês nascidos de partos gemelares e criados extensivamente no semiárido paraibano apresentam falha na transferência de imunidade passiva e alterações/diminuições marcantes nos teores séricos de alguns constituintes bioquímicos, suscitando a necessidade de interferência humana nestes casos.(AU)

This study is concerned with an evaluation of the influence of mode of birth on the transfer of passive immunity and some serum constituents to newborn lambs, naturally fed with colostrum, breed in the semiarid region of the State of Paraiba (Brazil) on an extensive system. Thirty-four clinically healthy Santa Ines lambs were employed, identified and weighted immediately after birth, and divided into two experimental groups of seventeen animals each. The PS group (nine males and eight females) included lambs born of single pregnancies and the PG group (six males and eleven females) comprised of lambs born of twin pregnancies. The colostrum intake occurred naturally and voluntarily at their mothers. Forty-eight hours after birth, 10 mL of blood were collected in siliconized tubes from each animal by puncture of the jugular vein, and maintained under vacuum. After centrifugation, aliquots of sera were separated and kept frozen at -15°C until analyzed. For the comparative study of serum constituents, two experimental groups were formed, distributed in a completely randomized lineation, 2x2 factorial (type of birth and sex). The data were subjected to analysis of variance, whose means were compared by Tukey test at 5%. The serum activities of AST and GGT, and the total protein serum, albumin, urea, creatinine, calcium, phosphorus and magnesium concentrations were determined by using sets of commercial reagents; the samples were read by an automatic spectrophotometer. The serum activities of AST, GGT and serum concentrations of total protein, albumin and globulins of the PS and PG lambs groups were not influenced by the type of pregnancy and sex. From the serum concentration of total protein, FTIP was found in the lambs of PG group, using the value of 5.0g/dL as the cutoff point. With the exception of calcium, serum concentrations of urea, creatinine, phosphorus and magnesium showed the same pattern. Although these constituents did not showed significant differences between groups and sex, higher values were observed in animals born of single pregnancies, suggesting that the absence of competition for the voluntary ingestion of colostrum may have been the determining factor. It can be concluded that lambs born twin deliveries and breed extensively in the semiarid region of the State of Paraiba failed to transfer passive immunity and showed significant reduction in serum levels of some biochemical constituents, indicating the need for human interference in these cases.(AU)

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Objetivou-se avaliar o efeito da suplementação sobre o consumo voluntário, digestibilidade aparente e desempenho produtivo de vacas Nelore em pastejo durante o periparto, além do peso ao nascimento e o desempenho ponderal das proles. Adicionalmente, buscou-se entender como se altera o consumo voluntário desses animais em torno do parto. Foram utilizadas 40 vacas pluríparas Nelore com peso médio de 525±46 kg, divididas em dois tratamentos: CON - animais recebendo apenas mistura mineral durante todo o período experimental e, SUP - animais recebendo mistura múltipla durante todo o período experimental. Determinou-se o consumo voluntário e a digestibilidade aparente dos animais ao longo do periparto (-45,-30,-15, +20, +40; períodos relativos à data estimada ao parto), através de ensaios de digestibilidade. Para avaliação de desempenho produtivo as vacas foram pesadas para calcular os ganhos médios diários (GMD) pré e pós-parto, os GMD em tecidos maternos e gestacionais durante o pré-parto e avaliadas quanto ao escore de condição corporal (ECC) ao parto e durante o pós-parto. As crias foram pesadas ao nascimento e aos 30 dias de idade. Os dados foram analisados utilizando o procedimento MIXED do SAS, adotando 0,10 como nível crítico de probabilidade de erro do tipo I. Verificou-se efeito de tratamento e período (dias relativos ao parto) para os consumos de matéria seca total, matéria orgânica e proteína bruta, enquanto o consumo de forragem foi similar (P 0,10) entre os tratamentos, mas com efeito de período (P 0.10). Houve diminuição de 14,37 % no consumo de matéria seca total do dia -30 para o -15 dia do pré-parto. No pósparto, verificou-se aos 20 dias de lactação, aumento de 72,66 % em relação ao período o dia - 15 do pré-parto. Houve interação entre período e tratamento para as digestibilidades aparentes da matéria orgânica (P 0.10), fibra insolúvel em detergente neutro corrigida para cinzas e proteínas (P 0.10) e proteína bruta (P 0.10). Não se observaram diferenças no GMD pósparto (P 0,10) e ECC ao parto (P 0.10) e pós-parto (P 0,10). Porém, foram verificados maiores GMD total (P 0.10) e em tecidos maternos (P 0.10) nos animais suplementados, mas com GMD em tecidos gestacionais (P 0,10) similar entre os tratamentos. Conclui-se que há uma diminuição do consumo em fêmeas gestantes a pasto ao aproximar do parto, independentemente da suplementação. A suplementação não altera esse padrão de consumo, mas aumenta o desempenho produtivo no pré-parto desses animais.

The objective was to evaluate the effect of supplementation on voluntary intake, apparent digestibility, and productive performance of grazing Nellore cows during peripartum, in addition to birth weight and performance of your offsprings. Additionally, it was sought to understand how the voluntary intake of these animals changes during the peripartum period. Forty multiparous Nellore cows with an average weight of 525±46 kg were used, divided into two treatments: CON - animals receiving only mineral mixture throughout the experimental period and SUP - animals receiving multiple mixture throughout the experimental period. Voluntary intake and apparent digestibility of the animals were determined throughout the peripartum period (-45,-30,-15, +20, +40; periods related to the estimated date of calving) through digestibility trial. For evaluation of productive performance, the cows were weighed to calculate the average daily gains (ADG) during pre and postpartum, ADG in maternal and gestational tissues during the prepartum and evaluated for the body condition score (BCS) at calving and during postpartum. The calves were weighed at birth and at 30 days of age. Data were analyzed using the MIXED procedures of SAS, adopting 0.10 as critical level of probability occurrence of type I error. Treatment and period effect (days related to calving) were verified for total dry matter intake, organic matter and crude protein, while forage intake was similar (P 0.10) among treatments, but with a period effect (P 0.10). There was a 14.37% decrease in total dry matter intake from day -30 to day -15 of prepartum. In the postpartum period, at 20 days of lactation, there was an increase of 72.66% in relation to the period day -15 of prepartum. There was interaction between period and treatment for apparent digestibilities of organic matter (P 0.10), neutral detergent fiber corrected for ash and protein (P 0.10) and crude protein (P 0.10). No differences were observed in postpartum ADG (P 0.10) and BCS at calving (P 0.10) and postpartum (P 0.10). However, higher total ADG (P 0.10) and in maternal tissues (P 0.10) were observed on supplemented animals, but with ADG in gestational tissues (P 0.10) similar among treatments. It is concluded that there is a decrease of voluntary intake in grazing pregnant cows when approaching calving, regardless of supplementation. Supplementation does not change this pattern of intake but increases the productive performance in the prepartum of these animals

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Nosso objetivo foi avaliar os efeitos da frequência de suplementação no desempenho e consumo voluntário de bovinos sob pastejo em pastagens tropicais utilizando abordagem meta-analítica. O banco de dados utilizado para avaliar o consumo voluntário foi compilado a partir de 19 experimentos conduzidos no Brasil entre 2009 e 2021, totalizando 75 médias de tratamento. Para avaliar o desempenho animal, foram coletadas médias de tratamento de 19 experimentos conduzidos no Brasil entre 2003 e 2018, totalizando 69 médias de tratamento. Os dados foram analisados utilizando técnicas de meta-análise, considerando o efeito aleatório de experimentos nos parâmetros do modelo. Não houve efeito da suplementação (P0,11) ou da frequência de suplementação (P0,18) no consumo de forragem e no conteúdo de matéria orgânica digerida da dieta (MOD dietética). Por outro lado, a suplementação aumentou (P<0,01) o consumo total, a PB dietética e a razão entre os conteúdos de PB dietética e MOD dietética (PB:DOM). A suplementação melhorou (P<0,05) o ganho médio diário (GMD), mas sem qualquer influência da frequência de suplementação (P0,84). O GMD aumentou linearmente (P>0,05) à medida que a PB da forragem aumentou, mas nenhuma diferença entre frequências de suplementação foi detectada. O ganho de peso adicional (GPA) causado pela suplementação diminui à medida que a PB da forragem aumenta e torna-se nulo a 150 g PB/kg de matéria seca. Houve padrão quadrático (P<0,01) do GPA em resposta a variações em ambos consumo de suplemento e consumo de PB suplementar, mas nenhum efeito das diferentes frequências foi detectado para essa variável (P>0,05). As máximas respostas ocorrem quando 5,0 g de suplemento e 1,14 g de PB suplementar foram fornecidas. A redução da frequência de suplementação ate três vezes na semana não afeta o desempenho e o consumo voluntário de bovinos sob pastejo em pastagens tropicais. Há resposta positiva à suplementação proteica no desempenho animal mesmo em forragens de alta qualidade. Contudo, essas respostas decrescem à medida que a proteína bruta da forragem aumenta e torna-se nula a 150 g PB/kg MS.

Our objective was to evaluate the effects of the supplementation frequency on performance and voluntary intake of cattle under grazing in tropical pastures by using a meta-analytical approach. The dataset used to evaluate the voluntary intake was compiled from 19 experiments carried out in Brazil between 2009 and 2021, totaling 75 treatment means. In order to evaluate the animal performance, treatment means were collected from 19 experiments carried out in Brazil between 2003 and 2018, totaling 69 treatment means. The data were analyzed by meta-analysis techniques, considering the random effect of the experiments on the model parameters. There was not effect of supplementation (P0.11) or supplementation frequency (P0.18) on forage intake and digested organic matter content in diet (dietary DOM). On the other hand, supplementation increased (P<0.01) total intake, dietary CP and ratio between dietary CP and dietary DOM contents (CP:DOM). The supplementation enhanced (P<0.05) the average daily gain (ADG), but without any influence of supplementation frequency (P0.84). The ADG increased linearly (P<0.05) as forage CP increased, but no difference (P>0.05) among supplementation frequencies was detected. The additional weight gain (AWG) caused by supplementation decreases as forage CP increases and becomes null at 150 g CP/kg dry matter. There was a quadratic pattern (P<0.01) of the AWG in response to variations in both supplement intake and supplemental CP intake, but none effect of different frequencies was detected on this variable (P>0.05). The maximal responses occur when 5.0 g of supplement and 1.14 g of supplemental CP per kg BW were provided. Reducing supplementation frequency down to thrice a week does not affect the voluntary intake and performance of cattle under grazing in tropical pastures. There is a positive response to protein supplementation on animal performance even with high-quality forages. However, those responses decreases as forage crude protein increases and becomes null at 150 CP/kg DM.