Silicon as an attenuator of the toxic effects of aluminum in Schinus terebinthifolius plants / Silício como atenuador dos efeitos tóxicos do alumínio em plantas de Schinus terebinthifolius

Aluminum (Al) is highly toxic to plants, since it causes stress and inhibits plant growth. Silicon (Si) is known to mitigate the stress caused by Al in several plant species. Thus, the current study aims to investigate the soothing effects of Si on morphophysiological and photosynthetic variables, and the attributes associated with oxidative stress in Schinus terebinthifolius plants exposed to Al. Treatments have followed a completely randomized design, with three repetitions based on the following Al/Si combinations (in mM): Treatment 1: 0 Al + 0 Si; Treatment 2: 0 Al + 2.5 Si; Treatment 3: 1.85 Al + 0 Si; Treatment 4: 1.85 Al + 2.5 Si; Treatment 5: 3.71 Al + 0 Si; Treatment 6: 3.71 Al + 2.5 Si. Each sampling unit consisted of a tray with 15 plants, totaling forty-five per treatment. Shoot and root morphological variables, photosynthetic variables, photosynthetic pigments, hydrogen peroxide concentration, lipid peroxidation (MDA), guaiacol peroxidase (POD) and superoxide dismutase (SOD) enzymes, and non-enzymatic antioxidant such as Ascorbic acid (AsA) and non-protein thiol (NPSH) concentration were assessed. Root growth inhibition followed by changes in root morphological variables have negatively affected root and shoot biomass production in plants only subjected to Al. However, adding 2.5 mM Si to the treatment has mitigated the toxic effects caused by 1.85 mM of aluminum on S. terebinthifolius plants.

O alumínio (Al) é altamente tóxico para as plantas, pois causa estresse e inibe o crescimento vegetal. O silício (Si) é conhecido por atenuar o estresse causado pelo Al em diversas espécies vegetais. Assim, o presente estudo tem como objetivo investigar os efeitos suavizantes do Si sobre variáveis morfofisiológicas e fotossintéticas, e os atributos associados ao estresse oxidativo em plantas de Schinus terebinthifolius expostas ao Al. Os tratamentos seguiram um delineamento inteiramente casualizado, com três repetições baseadas nas seguintes combinações Al/Si (em mM): Tratamento 1: 0 Al + 0 Si; Tratamento 2: 0 Al + 2,5 Si; Tratamento 3: 1,85 Al + 0 Si; Tratamento 4: 1,85 Al + 2,5 Si; Tratamento 5: 3,71 Al + 0 Si; Tratamento 6: 3,71 Al + 2,5 Si. Cada unidade amostral constou de uma bandeja com 15 plantas, totalizando quarenta e cinco plantas por tratamento. Variáveis morfológicas da parte aérea e da raiz, variáveis fotossintéticas, pigmentos fotossintéticos, concentração de peróxido de hidrogênio, peroxidação lipídica (MDA), enzimas guaiacol peroxidase (POD) e superóxido dismutase (SOD) e antioxidantes não enzimáticos como ácido ascórbico (AsA) e grupos tiós não protéicos (NPSH) foram avaliadas. A inibição do crescimento radicular seguida de alterações nas variáveis morfológicas radiculares afetaram negativamente a produção de biomassa radicular e aérea em plantas submetidas apenas ao Al. No entanto, a adição de 2,5 mM de Si ao tratamento atenuou os efeitos tóxicos causados por 1,85 mM de alumínio em plantas de S. terebinthifolius.

Woody species distribution across a savanna-dry forest soil gradient in the Brazilian Cerrado / Distribuição de espécies arbóreas em gradiente de solo savana-floresta seca no Cerrado brasileiro

Although richness and distribution of woody species in the Cerrado physiognomies have been extensively studied, the shifts of woody species from savanna physiognomies to dry forests have not yet been addressed. Here, we investigate the effect of soil physical-chemical traits on the woody species turnover between adjacent cerrado stricto sensu and dry forest physiognomies. Woody species were surveyed, and soil and topographic variables measured, in 30 10×40 m plots systematically distributed, with 15 plots in each physiognomy. We found a spatially structured distribution of woody species, and differences of soil traits between cerrado stricto sensu and dry forest areas, mainly related to the aluminum saturation, base saturation, and available phosphorus. Aluminum saturation increased toward the savanna area, while base saturation increased toward the dry forest. Most woody species predominated in one physiognomy, such as Callisthene major in the cerrado stricto sensu and Anadenanthera colubrina in the dry forest. Only 20% of the species were widely distributed across both physiognomies or, not often, restricted to the intermediary values of the soil gradient. General results indicate that contrasting soil traits between cerrado stricto sensu and dry forest produce a strongly spatially organized and sharp transition in terms of species distribution between these physiognomies.

Embora a distribuição e a riqueza em espécies arbóreas nas fitofisionomias do Cerrado venham sendo bastante estudadas, a transição entre savanas e florestas deciduais ainda não foi abordada. Investigamos o efeito de características físico-químicas do solo sobre a distribuição de espécies arbóreas em região de contato entre cerrado sentido restrito e floresta estacional decidual (FED). As espécies arbóreas foram amostradas sistematicamente, e variáveis de topografia e características do solo foram medidas em 30 parcelas de 10×40 m, sendo 15 parcelas em cada fisionomia. A distribuição das espécies arbóreas foi espacialmente estruturada, e as características do solo diferiram entre as áreas de cerrado sentido restrito e FED, principalmente relacionadas à saturação de alumínio, saturação de bases e teores de fósforo. A saturação de alumínio aumentou em direção ao cerrado sentido restrito, enquanto a saturação de bases aumentou em direção à FED. A maioria das espécies arbóreas predominou em uma das fisionomias, como Callisthene major em cerrado sentido restrito e Anadenanthera colubrina em FED. Apenas 20% das espécies foram amplamente distribuídas em ambas as fisionomias ou, em poucos casos, restritas aos valores intermediários do gradiente de solo. Os resultados indicam um forte contraste de características do solo entre o cerrado sentido restrito e a FED, assim como uma transição acentuada e espacialmente organizada quanto à distribuição de espécies arbóreas.

Utilização de caneca de alumínio como material alternativo para temperatura de globo de Vernon / Use of aluminum mug as alternative material for Vernon globe temperature

Diversos estudos relacionados à viabilidade e eficiência do uso de materiais alternativos ao globo de Vernon têm sido realizados, principalmente em decorrência ao preço elevado do cobre, componente do instrumento padrão. O objetivo foi avaliar a eficiência da utilização de caneca de alumínio como material alternativo para substituição do globo de Vernon. Para a confecção do protótipo empregou-se uma caneca de alumínio, utilizada em pistola de pintura, com capacidade de 50 ml. A caneca foi pintada com tinta preto-fosca, em duas camadas e um termômetro de mercúrio foi inserido ao centro. Para realização dos testes foram utilizados quatro equipamentos (dois globos de Vernon e dois globos negros alternativos). Foram efetuados testes prévios de comportamento temporal. Estabelecido o tempo, as temperaturas dos globos foram coletadas durante sete dias consecutivos, em três períodos, de 15 minutos cada. Os registros de temperatura foram realizados a cada cinco minutos, num total de 84 por equipamento. Os dados foram submetidos a análise de correlação de Pearson. Os resultados indicaram correlação positiva com o r2 de 0,9927, estabelecendo uma forte correlação entre os resultados de temperatura média do globo negro alternativo e globo de Vernon. Conclui-se que o globo negro alternativo do tipo caneca de alumínio mostrou-se eficiente para aferição da temperatura de globo negro em estudos de ambiência em substituição ao Globo de Vernon.(AU)

Several studies related to the feasibility and efficiency of using alternative materials to Vernon's globe have been carried out, mainly due to the high price of copper, a component of the standard instrument. The objective was to evaluate the efficiency of using an aluminum mug as an alternative material for replacing Vernon's globe. To make the prototype, an aluminum mug was used, used in a paint gun, with a capacity of 50 ml. The mug was painted with matte black paint, in two layers and a mercury thermometer was inserted in the center. Four pieces of equipment were used to carry out the tests (two Vernon globes and two alternative black globes). Previous temporal behavior tests were carried out. Once the time was established, the globes' temperatures were collected for seven consecutive days, in three periods of 15 minutes each. Temperature records were taken every five minutes, a total of 84 per equipment. Data were subjected to Pearson's correlation analysis. The results indicated a positive correlation with the r2 of 0.9927, establishing a strong correlation between the mean temperature results of the alternative black globe and Vernon's globe. It is concluded that the alternative black globe of the aluminum mug type proved tobe efficient for measuring the temperature of the black globe in ambience studies, replacing the Vernon Globe.(AU)

Woody species distribution across a savanna-dry forest soil gradient in the Brazilian Cerrado / Distribuição de espécies arbóreas em gradiente de solo savana-floresta seca no Cerrado brasileiro

Although richness and distribution of woody species in the Cerrado physiognomies have been extensively studied, the shifts of woody species from savanna physiognomies to dry forests have not yet been addressed. Here, we investigate the effect of soil physical-chemical traits on the woody species turnover between adjacent cerrado stricto sensu and dry forest physiognomies. Woody species were surveyed, and soil and topographic variables measured, in 30 10×40 m plots systematically distributed, with 15 plots in each physiognomy. We found a spatially structured distribution of woody species, and differences of soil traits between cerrado stricto sensu and dry forest areas, mainly related to the aluminum saturation, base saturation, and available phosphorus. Aluminum saturation increased toward the savanna area, while base saturation increased toward the dry forest. Most woody species predominated in one physiognomy, such as Callisthene major in the cerrado stricto sensu and Anadenanthera colubrina in the dry forest. Only 20% of the species were widely distributed across both physiognomies or, not often, restricted to the intermediary values of the soil gradient. General results indicate that contrasting soil traits between cerrado stricto sensu and dry forest produce a strongly spatially organized and sharp transition in terms of species distribution between these physiognomies.(AU)

Embora a distribuição e a riqueza em espécies arbóreas nas fitofisionomias do Cerrado venham sendo bastante estudadas, a transição entre savanas e florestas deciduais ainda não foi abordada. Investigamos o efeito de características físico-químicas do solo sobre a distribuição de espécies arbóreas em região de contato entre cerrado sentido restrito e floresta estacional decidual (FED). As espécies arbóreas foram amostradas sistematicamente, e variáveis de topografia e características do solo foram medidas em 30 parcelas de 10×40 m, sendo 15 parcelas em cada fisionomia. A distribuição das espécies arbóreas foi espacialmente estruturada, e as características do solo diferiram entre as áreas de cerrado sentido restrito e FED, principalmente relacionadas à saturação de alumínio, saturação de bases e teores de fósforo. A saturação de alumínio aumentou em direção ao cerrado sentido restrito, enquanto a saturação de bases aumentou em direção à FED. A maioria das espécies arbóreas predominou em uma das fisionomias, como Callisthene major em cerrado sentido restrito e Anadenanthera colubrina em FED. Apenas 20% das espécies foram amplamente distribuídas em ambas as fisionomias ou, em poucos casos, restritas aos valores intermediários do gradiente de solo. Os resultados indicam um forte contraste de características do solo entre o cerrado sentido restrito e a FED, assim como uma transição acentuada e espacialmente organizada quanto à distribuição de espécies arbóreas.(AU)

Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate

This study describes a biotechnological strategy for producing and applying oxalic acid to solubilize phosphorus (P) from rock phosphate (RP). We evaluated six fungal species (Aspergillus niger FS1, Penicillium islandicum FS41, Pleurotus ostreatus PO1, Rhizoctonia solani Rhiz555, Sclerotium rolfsii Sr25, and Sclerotinia sclerotiorum Scl134) and three culture media (potato dextrose broth, Tsao and Strasser media) to maximize oxalic acid production. Among the fungal isolates tested and culture media, S. rolfsii Sr25 and Tsao medium showed efficient oxalic acid production. Tsao medium was optimized following a response surface methodology after initial screening of factors affecting RP solubilization. The optimized concentrations were 1 g L-1 NaNO3, 100 g L-1 glucose, 2 g L-1 KH2PO4, 4.5 g L-1 yeast extract, and 25 mg L-1 MgSO4·7H2O used for 20 days of incubation. Under these conditions, 71 mmol L-1 oxalic acid was obtained, representing a three-fold increase over production under non-optimized conditions (20 mmol L-1). Under optimized conditions, oxalic acid produced by S. rolfsii Sr25 reacted with low-solubility RP and solubilized 100 % of the P contained in ore. Thus, using S. rolfsii Sr25 to produce oxalic acid seems a promising biotechnological alternative for P solubilization from RP.(AU)

Fish functional responses to local habitat variation in streams within multiple land uses areas in the Amazon

In this study, we assessed the effects of multiple land uses and local habitat variables on the composition of fish functional trophic groups (FTG's) and on the ecomorphological traits of fish in Amazonian streams. We evaluated land use types and local habitat variables in 26 streams distributed within a land use gradient. Land use and habitat variables affected the composition of FTG's, as evidenced by the increased abundance of diurnal channel drift feeders in areas with high dissolved oxygen and deeper thalweg. At the same time, diurnal surface pickers, as well as diggers, and ambush and stalker predators were more abundant in streams with higher canopy density. Only habitat variables affected the ecomorphological characteristics of the species. Fish with higher values of relative caudal peduncle length were positively associated with high canopy density, while fish with greater relative mouth width were negatively associated with the variables impact in the riparian zone and cover of fish shelter. The stream fish functional structure was mainly affected by the impacts caused to the local habitat resulting from different land uses. Thus, preserving forest remnants, as well as recovering degraded areas, is essential for the maintenance of aquatic biodiversity in the region.(AU)

Neste estudo, avaliamos os efeitos dos múltiplos usos da terra e de variáveis do habitat local sobre a composição de grupos tróficos funcionais (FTG's), e nos traços ecomorfológicos, de peixes em riachos amazônicos. Analisamos os tipos de uso da terra e variáveis da estrutura do habitat local em 26 riachos distribuídos dentro de um gradiente de uso da terra. O uso da terra e as variáveis de habitat afetaram a composição de FTG's, evidenciado pelo aumento na abundância de peixes diurnos que se alimentam de organismos em suspensão na água em áreas com maiores valores de oxigênio dissolvido e profundidade do talvegue. Ao passo que os peixes diurnos catadores de superfície, bem como os escavadores e predadores de emboscada foram mais abundantes em riachos com maior densidade florestal. Apenas as variáveis do habitat afetaram as características ecomorfológicas das espécies. Peixes com maiores valores de comprimento relativo do pedúnculo caudal foram associados positivamente com alta densidade do dossel, enquanto peixes que apresentam maior largura relativa da boca foram associados negativamente com as variáveis impacto na zona ripária e abrigo para peixes. A estrutura funcional da comunidade de peixes de riachos foi afetada principalmente pelos impactos causados ao habitat decorrentes de diferentes usos da terra. Portando, preservar os remanescentes florestais, bem como recuperar áreas já degradadas, é fundamental para a manutenção da biodiversidade aquática na região.(AU)

Root exudation of oxalic acid in Lotus corniculatus in response to aluminum toxicity

The objective of this research was to identify the existence of root exudation of organic acid in Lotus corniculatus germplasms subjected to toxic aluminum (Al) levels and investigate the effect of this mechanism on the Al content in the root tissue and in morphological parameters of plant development. Two experiments were performed in nutrient solution to evaluate the Al accumulation and exudation of organic acids, using cultivars INIA Draco and São Gabriel and genotypes UFRGS and UF-T2. The plants were cultivated in Al-free solution, which was applied on the 45th day in half of the pots of each genotype. Root exudation was highly correlated with the reduced accumulation of Al in the root tissue (r2 = 0.75 at 72 h). Genotype UF-T2, selected for Al tolerance, extruded 80% more oxalic acid in the presence of Al compared with the other germplasms, indicating that this mechanism is involved with Al tolerance in L. corniculatus. This experiment showed strong evidence that L. corniculatus exhibits Type I tolerance, in which anionic channels are rapidly activated by Al exposure. Exudation of oxalic acid is likely a crucial mechanism that allows the maintenance of L. corniculatus growth when exposed to toxic Al conditions, and this characteristic should be used to identify tolerant genotypes in the future.(AU)

Efficacy of Aluminum Sulfate Addition to Poultry Litter on Productive Performance of Laying Hens, Ammonia Emissions, and Litter Quality

The objective of this research was to examine whether different types of litter (New, used, and mixed) and alum could interfere in litter quality, thereby also influencing the productive performance of laying hens. A total number of 450 Inshas chickens aged 24 weeks were randomly distributed into six groups (5 replicates, each with 15 hens) of floor litter. The groups included (G1) new wheat straw litter, (G2) used wheat straw litter, (G3) mixed wheat straw litter (50% new+50% used) and (4, 5 and 6) the same litter types in groups 1, 2 and 3 supplied with 500g of alum /m2 litter. The experiment lasted 16 weeks. Laying hens productive parameters, blood parameters, carcass parameters, respiratory rate, coliform count and pH, as well as ammonia emission, litter quality were assessed.The results showed that the litter type significantly affects the level of ammonia emission, litter characteristics, carcass characteristics, intestinal pH and coliform count, blood characteristics (T3, cholesterol, uric acid, total protein, albumin, globulin, hemoglobin, and PCV), as well as the body weight gain. In general, we found that reused litter followed by the mixed litter without added alum resulted in the lowest efficiency on the studied traits, as compared to the same types of litter treated with alum and the new litter. We therefore conclude that the addition of alum reduces ammonia emission and improves the characteristics of the litter, which is reflected in enhancement of productive performance and properties of blood and carcass.(AU)

Resveratrol-Tempeh reduce micronucleus frequencies bone marrow cells and stimulate osteocyte proliferation in aluminum chloride-induced mice / Redução de frequências de micronúcleos células da medula óssea e estímulo proliferação de osteócitos em camundongos induzidos por cloreto de alumínio através do uso de Resveratrol-Tempeh

Aluminum (Al) is widely used for water purification, cooking pots, cosmetic and pharmaceutical preparations, toothpaste tubes, and food processing industries. Although the transport in the digestive tract is very poor but if the load is high, it can be absorbed and accumulated. About 50-70% of Al accumulates in the bones and can have an impact on human health. Resveratrol (RES), isolated from tempeh as an Indonesian food ingredient, can increase cell viability and has promising cytoprotective effects. RES has the capacity to interact with oxidative stress, so it has the potential as a therapy in bone repair. Therefore, this study aimed to evaluate the effect of RES on the number of osteocytes and bone marrow cells in Al-induced mice. Swiss Webster mice were divided into four groups: (1) untreated groups, (2) AlCl3 -treated groups, (3) Al+Res5 treated groups, and (4) Al+Res10 treated groups. Al dose 200 mg/kg body weight was administered intraperitoneally. RES was given one hour after administration of Al, with doses of 5 and 10 mg/kg Body Weight. Al and RES administration is carried out for one month. All mice were sacrificed, and mouse bones were isolated for histological preparations and a half for genotoxic assays. Bone marrow cells were collected and stained with My Grunwald. The number of micronuclei polychromatic erythrocytes (MNPCE) was examined in 1,000 PCEs per animal. The number of PCEs is counted by at least 200 erythrocytes (PCE + NCE) per animal. The results showed that the administration of Al significantly increased the number of micronuclei (MN) but after administration of RES at doses of 5 and 10 mg/kg Body Weight significantly reduced the number of MN in bone marrow cells. A dose of RES 10 mg/kg BW stimulates proliferation and increases the number of osteocytes in bone significantly. It can be concluded that Al can cause genotoxicity in bone marrow cells and RES is anti-genotoxic and can stimulate osteocyte proliferation.

O alumínio (Al) é amplamente utilizado para purificação de água, panelas, preparações cosméticas e farmacêuticas, tubos de pasta de dente e indústrias de processamento de alimentos. Embora o transporte no trato digestivo seja escasso, se a carga for alta, pode ser, todavia, absorvida e acumulada. Cerca de 50-70% do Al se acumula nos ossos e pode ter impacto na saúde humana. O resveratrol (RES), isolado do tempê indonésio como ingrediente alimentar, pode aumentar a viabilidade celular e tem efeitos citoprotetores promissores. O RES possui a capacidade de interagir com o estresse oxidativo, e por essa razão pode ser utilizado como terapia no reparo ósseo. Portanto, este estudo teve como objetivo avaliar o efeito do RES no número de osteócitos e células da medula óssea em camundongos induzidos por Al. Camundongos Swiss Webster (SW) foram divididos em quatro grupos: (1) grupos não tratados, (2) grupos tratados com AlCl3 , (3) grupos tratados com Al+Res5 e (4) grupos tratados com Al+Res10. Uma dose de 200 mg/kg de peso corporal foi administrada por via intraperitoneal. O RES foi administrado uma hora após a administração do Al, nas doses de 5 e 10 mg/kg de peso corporal. A administração de Al e RES foi realizada por um mês. Todos os camundongos foram sacrificados, e os ossos dos camundongos foram isolados para preparações histológicas e meio para ensaios genotóxicos. As células da medula óssea foram coletadas e coradas com My Grunwald. O número de eritrócitos policromáticos micronúcleos (MNPCE) foi examinado em 1.000 PCEs por animal. O número de PCEs foi contado por pelo menos 200 eritrócitos (PCE + NCE) por animal. Os resultados mostraram que a administração de Al aumentou significativamente o número de micronúcleos (MN), mas após a administração de RES nas doses de 5 e 10 mg/kg de peso corporal reduziu significativamente o número de MN nas células da medula óssea. Uma dose de RES de 10 mg/kg BW estimula a proliferação e aumenta significativamente o número de osteócitos no osso. Dessa forma, pôde-se concluir que o Al pode causar genotoxicidade em células da medula óssea e o RES é antigenotóxico e pode estimular a proliferação de osteócitos.

Relations between soil attributes and the abundance of Bacillus thurigiensis in the Cerrado of Maranhão state, Brazil / Relações entre atributos do solo e a abundância do Bacillus thurigiensis no Cerrado do estado do Maranhão, Brasil

The influence of abiotic factors on the abundance of microorganism populations in soil has been sparsely studied, especially regarding Bacillus thuringiensis (Bt) bacteria. Therefore, this research was aimed at analyzing the relationship between the chemical and textural characteristics of the soil of the Cerrado (savanna) of Maranhão State on the abundance of Bacillus thuringiensis. Soil samples were collected in different municipalities in eastern Maranhão: São Mateus do Maranhão, Alto Alegre, Coroatá, Timbiras and Codó. The soil samples were obtained in the 0-0.1 m layer for soil fertility and texture analysis. Then, in the same area for the isolation of Bt, 1 g of soil was collected. The colonies obtained in the isolation that featured morphological characteristics of Bacillus spp. were visualized under phase contrast microscopy. Principal component analysis, clustering and correlations were peformed. Results: The sand content correlated positively with the Bacillus thuringiensis index (iBt). The cluster analysis allowed for verifying that the soils not showed iBt in function of high concentrations of aluminum (Al) and potential acidity (H+Al). Considering as these attributes (Al and H+Al) alter the availability of P in the soil, the abundance of Bacillus thuringiensis may have been impaired by the deficiency of this element in the environment. Conclusion: Bt has correlations with soil texture, and high concentrations of aluminum and potential acidity in the soil influencing the permanence of Bacillus thuringiensis in Maranhão eastern Cerrado.

A influência de fatores abióticos sobre a abundância de populações de microrganismos no solo tem sido pouco estudada, principalmente com relação à bactéria Bacillus thuringiensis (Bt). Assim, objetivou-se analisar a relação entre as características químicas e texturais do solo do Cerrado maranhense na abundância de Bacillus thuringiensis. As coletas de solo foram realizadas em municípios do leste maranhense: São Mateus do Maranhão, Alto Alegre, Coroatá, Timbiras e Codó. As amostras de solo foram obtidas na camada de 0-0.1 m, para análise da fertilidade do solo e sua textura. As colônias obtidas no isolamento que apresentaram características morfológicas de Bacillus spp. foram visualizadas sob microscopia de contraste de fase. Em seguida, na mesma área para o isolamento do Bt, foi coletado 1g de solo. De posse dos dados procedeu-se análise de componentes principais, agrupamentos e correlações. Resultados: A areia correlacionou-se positivamente com o índice de Bacillus thuringiensis (iBt). A análise de agrupamento permitiu verificar que os solos que não apresentaram iBt, possuíam altas concentrações de alumínio e acidez potencial (H+Al). Como estes atributos alteram a disponibilidade de P no solo, a abundância de Bacillus thuringiensis pode ter sido prejudicada pela deficiência deste elemento no ambiente. Conclusão: Bt possui correlações com a textura do solo, e altas concentrações de alumínio e acidez potencial no solo influenciam na permanência de Bacillus thuringiensis no Cerrado do leste maranhense.

Concentration and moment of niacin application on Urochloa brizantha in relation to nitrogen fertilization / Concentração e momento de aplicação de niacina em Urochloa brizantha em relação à adubação nitrogenada

The present study aimed to evaluate the effect of applying different concentrations of niacin, at different times in relation to nitrogen fertilization, on the characteristics of Urochloa brizantha. The treatments consisted of applying three concentrations of niacin (0, 100, and 200 mg L-1) and three different moments (two days before, together, and two days after nitrogen fertilization). It was found that there was variation in response, mainly concerning the moment of application of the vitamins, in which the joint and subsequent application of fertilization generally exceeded the application performed previously, and of both concentrations used (100 and 200 mg L-1), in relation to the control without vitamin application. In this way, the application of niacin at a concentration of 100 mg L-1, carried out jointly or after nitrogen fertilization, provides better conditions for developing and producing dry matter of Urochloa brizantha due to the presence of nitrogen.

O presente estudo teve como objetivo avaliar o efeito da aplicação de diferentes concentrações de niacina, em momentos distintos em relação à adubação nitrogenada, sobre as características de Urochloa brizantha. Os tratamentos foram compostos pela aplicação de três concentrações de niacina (0, 100 e 200 mg L-1) e três momentos distintos (dois dias antes, juntamente e dois dias após a adubação nitrogenada). Verificou-se que houve variação de resposta, principalmente em relação ao momento de aplicação das vitaminas, em que as aplicações conjunta e posterior à adubação superaram, de maneira geral, a aplicação realizada anteriormente, e de ambas as concentrações utilizadas (100 e 200 mg L-1), em relação ao controle sem vitamina. Desta maneira, a aplicação de niacina em concentração de 100 mg L-1, realizada de maneira conjunta ou posterior à adubação nitrogenada, propicia melhores condições para o desenvolvimento e a produção de matéria seca de Urochloa brizantha, devido à presença do nitrogênio.

Phytochemical screening and evaluation of antioxidant, total phenolic and flavonoid contents in various weed plants associated with wheat crops / Triagem fitoquímica e avaliação dos teores de antioxidantes, fenólicos totais e flavonoides em várias plantas daninhas associadas a culturas de trigo

This study explores the antioxidant activity, phytochemical screening, total phenolic and flavonoids contents in the extracts of four locally available weeds plants namely Convolvulus arvensis, Chenopodium murale, Avena fatua and Phalaris minor with different solvents. The antioxidant activities of these extracts were determined via various in-vitro methods such as total antioxidant activity (TAA), reducing power (RP), DPPH (2,2-Diphenyl-1- Picrylhydrazyl) free radical scavenging and hydrogen peroxide scavenging assays. Phytochemical screening was performed both qualitatively as well as quantitatively. Total phenolic content (TPC) and total flavonoid content (TFC) were determined through Folin- Ciocalteu reagent and aluminium chloride methods respectively. Methanolchloroform solvent showed the presence of a high amount of TPC in milligram of gallic acid equivalent per gram of dry weight (mg of GAE/g of DW) in the extracts of all weeds. Their descending sequence was Avena fatua (74.09) ˃ Phalaris minor (65.66) ˃ Chenopodium murale (64.04) ˃ Convolvulus arvensis (61.905), while, chloroform solvent found to be best solvent for the extraction of TFC. Methanol-chloroform solvent was also found to be best solvent for TAA (Total antioxidant activity assay) which showed values in milligram of ascorbic acid equivalent per gram of dry weight (mg of AAE /g of DW), for DPPH scavenging activity, reducing power (antioxidant activity) and hydrogen peroxide scavenging activity. Phytochemical screening indicated the presence of polyphenols, flavonoids, tannins, saponins, alkaloids and glycosides in these weeds.

Este estudo investiga a atividade antioxidante, a triagem fitoquímica, os teores de fenólicos totais e de flavonoides nos extratos de quatro plantas daninhas disponíveis localmente, quais sejam, Convolvulus arvensis, Chenopodium murale, Avena fatua e Phalaris minor com diferentes solventes. As atividades antioxidantes desses extratos foram determinadas por meio de vários métodos in vitro, tais como atividade antioxidante total (TAA), poder redutor (RP), sequestro de radicais livres DPPH (2,2-Difenil-1-Picril-hidrazil) e ensaios de sequestro de peróxido de hidrogênio. A triagem fitoquímica foi realizada tanto qualitativamente quanto quantitativamente. O teor de fenólicos totais (TPC) e o teor de flavonoides totais (TFC) foram determinados pelos métodos do reagente de Folin-Ciocalteu e do cloreto de alumínio, respectivamente. O solvente metanol-clorofórmio mostrou a presença de elevada quantidade de TPC em miligramas de ácido gálico equivalente por grama de peso seco (mg de GAE/g de DW) nos extratos de todas as plantas daninhas. Sua sequência descendente foi Avena fatua (74,09) ˃ Phalaris minor (65,66) ˃ Chenopodium murale (64,04) ˃ Convolvulus arvensis (61,905), enquanto o solvente clorofórmio foi o melhor solvente para a extração de TFC. O solvente metanol-clorofórmio também foi considerado o melhor solvente para AAT (ensaio de atividade antioxidante total), que apresentou valores em miligramas de equivalente de ácido ascórbico por grama de peso seco (mg de AAE/g de DW), para atividade sequestrante de DPPH, RP (atividade antioxidante) e atividade de sequestro de peróxido de hidrogênio. A triagem fitoquímica indicou a presença de polifenóis, flavonoides, taninos, saponinas, alcaloides e glicosídeos nessas plantas daninhas.

Light-emitting diode stimulates radiodermatitis recovery

Purpose To evaluate the effect of light-emitting diode (LED) in an experimental model of radiodermatitis. Methods Ten male Wistar rats weighing 200250 g were analyzed. Radiation was delivered in a single dose (20 Gy with Strontium-90 dermatological plaques), two areas per animal. After 15 days, they were divided into two groups: control group (n = 5) and LED group (n = 5), which was treated during 21 days later (LED 660 nm, 10 min in alternate days). The endpoints were radiodermatitis scale, histological analysis HE, Picrius Sirius and the gene expression of interleukin-10 (IL-10) and matrix metalloproteinase-9 (MMP-9). Results The LED group showed a higher number of dermal appendages (p = 0.04) and angiogenesis(p = 0.007), a tendency towards higher IL-10 (p = 0.06) and an increase in MMP-9 (p = 0.004) when compared to the control group. Conclusions This study suggested that the use of LED for radiodermatitis increased skin regeneration.(AU)

Sombric-like horizon and xanthization in polychrome subtropical soils from Southern Brazil: implications for soil classification

The occurrence of dark subsurface horizons rich in organic matter (OM) associated with polychrome in the B horizon (yellowish over reddish hue) is common in soils from Southern Brazil. The formation of these horizons and the combination with such morphological attributes has not been properly documented, and neither has the cause effect relationship. Four soil profiles with such sombric-like horizons with a yellowish color at the upper part of the B horizon over red subsoil were studied in Southern Brazil. Results from micromorphology, extractable sesquioxide minerals, clay mineralogy and isomorphic substitution of Fe by Al in iron minerals showed that melanization, xanthization, bioturbation, moderate shrinking/swelling and moderate ferralitization were the most evident pedogenetic processes in role. Xanthization is closely related to the sombric-like horizon formation. In the studied area the findings demonstrated that no clay and OM illuviation had taken place. Therefore, the classification of these soils was revisited, so as to take into account the processes that underlie their genesis with emphasis on xanthization, clay illuviation and soil aggregation. The results suggest that the sombric horizon may need redefinition, unless profiles can be found in which illuviation of clay and/or OM can be proven.(AU)

Sombric-like horizon and xanthization in polychrome subtropical soils from Southern Brazil: implications for soil classification

The occurrence of dark subsurface horizons rich in organic matter (OM) associated with polychrome in the B horizon (yellowish over reddish hue) is common in soils from Southern Brazil. The formation of these horizons and the combination with such morphological attributes has not been properly documented, and neither has the cause effect relationship. Four soil profiles with such sombric-like horizons with a yellowish color at the upper part of the B horizon over red subsoil were studied in Southern Brazil. Results from micromorphology, extractable sesquioxide minerals, clay mineralogy and isomorphic substitution of Fe by Al in iron minerals showed that melanization, xanthization, bioturbation, moderate shrinking/swelling and moderate ferralitization were the most evident pedogenetic processes in role. Xanthization is closely related to the sombric-like horizon formation. In the studied area the findings demonstrated that no clay and OM illuviation had taken place. Therefore, the classification of these soils was revisited, so as to take into account the processes that underlie their genesis with emphasis on xanthization, clay illuviation and soil aggregation. The results suggest that the sombric horizon may need redefinition, unless profiles can be found in which illuviation of clay and/or OM can be proven.

Bioaccumulation of metals and evaluation of golden mussels encrusted on different screens of net cages / Bioacumulação de metais e avaliação do mexilhão-dourado incrustado em diferentes telas de tanques-rede

This study aimed to evaluate the characterization and bioaccumulation of metals of the golden mussel encrusted in the screens of net cages installed in the reservoir of Itaipu Binacional. The experimental design was completely randomized, composed of two types of screens (PVC and Bezinal) distributed on four sides (Sides I, II, III, and IV) of the net cage. The total mass, the average mass, and the dimensions (width, height, and length) of the mollusks were evaluated. The mussels adhered to the Bezinal screen presented a higher average mass, length, height, and width than those colonized on the PVC screen (p <0.05). However, the PVC screen provided a greater (p <0.05) total mass of encrusted mussels. Regardless of the screen used, the average mass and length of mussels had higher values on Side I (p <0.05) than to the Sides II and IV, but not different from Side III. The mussels evaluated showed high levels of metals, and those encrusted in the Bezinal screen presented higher levels of Al, Zn and Cr than the individuals on the PVC screen (p <0.05). The use of a Bezinal screen is more efficient than a PVC screen, as an antifouling material. Mussels with shorter lengths had a higher incidence on the PVC screen. The mussels encrusted in the Bezinal screen bioaccumulate higher content of Al, Zn, and Cr. The type of screen used in the net cages influences the mass and size of adhered mussels, as well as can interfere with the metal accumulation in the golden mussel.

Objetivou-se avaliar a caracterização e a bioacumulação de metais do mexilhão-dourado incrustado nas telas de tanques-rede instalados no reservatório da Itaipu Binacional. O delineamento experimental foi inteiramente casualizado, composto por dois tipos de telas (PVC e Bezinal) distribuídas em quatro lados (Lados I, II, III e IV) do tanque-rede. Foram avaliadas a massa total, a massa média e as dimensões (largura, altura e comprimento) dos moluscos. Os mexilhões aderidos na tela Bezinal apresentaram maior massa média, comprimento, altura e largura do que aqueles colonizados na tela PVC (p <0,05). No entanto, a tela PVC proporcionou uma maior (p <0,05) massa total de mexilhões incrustados. Independente da tela utilizada, a massa média e o comprimento de mexilhões apresentaram maiores valores no Lado I (p <0,05) em relação aos Lados II e IV, mas não diferindo do Lado III. Os mexilhões avaliados apresentaram níveis elevados de metais, sendo que os incrustados à tela Bezinal revelaram teores mais elevados de Al, Zn e Cr do que os indivíduos na tela PVC (p <0,05). O uso da tela Bezinal é mais eficiente em relação à tela PVC como material anti-incrustante. Os mexilhões com menores comprimentos tiveram uma maior incidência na tela PVC. Os mexilhões incrustados à tela Bezinal bioacumulam maior teor de Al, Zn e Cr. O tipo de tela utilizado nos tanques-rede influencia na massa e tamanho dos mexilhões aderidos, bem como pode interferir na acumulação de metais no mexilhão-dourado.

Bioaccumulation of metals and evaluation of golden mussels encrusted on different screens of net cages / Bioacumulação de metais e avaliação do mexilhão-dourado incrustado em diferentes telas de tanques-rede

This study aimed to evaluate the characterization and bioaccumulation of metals of the golden mussel encrusted in the screens of net cages installed in the reservoir of Itaipu Binacional. The experimental design was completely randomized, composed of two types of screens (PVC and Bezinal) distributed on four sides (Sides I, II, III, and IV) of the net cage. The total mass, the average mass, and the dimensions (width, height, and length) of the mollusks were evaluated. The mussels adhered to the Bezinal screen presented a higher average mass, length, height, and width than those colonized on the PVC screen (p <0.05). However, the PVC screen provided a greater (p <0.05) total mass of encrusted mussels. Regardless of the screen used, the average mass and length of mussels had higher values on Side I (p <0.05) than to the Sides II and IV, but not different from Side III. The mussels evaluated showed high levels of metals, and those encrusted in the Bezinal screen presented higher levels of Al, Zn and Cr than the individuals on the PVC screen (p <0.05). The use of a Bezinal screen is more efficient than a PVC screen, as an antifouling material. Mussels with shorter lengths had a higher incidence on the PVC screen. The mussels encrusted in the Bezinal screen bioaccumulate higher content of Al, Zn, and Cr. The type of screen used in the net cages influences the mass and size of adhered mussels, as well as can interfere with the metal accumulation in the golden mussel.(AU)

Objetivou-se avaliar a caracterização e a bioacumulação de metais do mexilhão-dourado incrustado nas telas de tanques-rede instalados no reservatório da Itaipu Binacional. O delineamento experimental foi inteiramente casualizado, composto por dois tipos de telas (PVC e Bezinal) distribuídas em quatro lados (Lados I, II, III e IV) do tanque-rede. Foram avaliadas a massa total, a massa média e as dimensões (largura, altura e comprimento) dos moluscos. Os mexilhões aderidos na tela Bezinal apresentaram maior massa média, comprimento, altura e largura do que aqueles colonizados na tela PVC (p <0,05). No entanto, a tela PVC proporcionou uma maior (p <0,05) massa total de mexilhões incrustados. Independente da tela utilizada, a massa média e o comprimento de mexilhões apresentaram maiores valores no Lado I (p <0,05) em relação aos Lados II e IV, mas não diferindo do Lado III. Os mexilhões avaliados apresentaram níveis elevados de metais, sendo que os incrustados à tela Bezinal revelaram teores mais elevados de Al, Zn e Cr do que os indivíduos na tela PVC (p <0,05). O uso da tela Bezinal é mais eficiente em relação à tela PVC como material anti-incrustante. Os mexilhões com menores comprimentos tiveram uma maior incidência na tela PVC. Os mexilhões incrustados à tela Bezinal bioacumulam maior teor de Al, Zn e Cr. O tipo de tela utilizado nos tanques-rede influencia na massa e tamanho dos mexilhões aderidos, bem como pode interferir na acumulação de metais no mexilhão-dourado.(AU)

A utilização do laser de baixa intensidade na cicatrização de ferida em cão / The use of low intensity laser in wound healing in dog

Background: Low-intensity laser is effective in cellular metabolism, analgesia, and tissue repair. The bioelectric, bioenergetic, and biochemical effects of laser therapy stimulate local circulation, collagen formation, and epithelization. The objective of this study was to report the use of gallium-aluminum arsenide (GaAlAs) laser (830 nm) in healing two woundscaused by necrosis in a dog. Case: An 8-year-old bitch, a rescued victim of animal abuse, had tibiotarsal dislocation in the left posterior limb. Orthopedic surgery was performed with the placement of an external fixation device with six pins in the distal third of the left posterior limb. A radiographic examination performed 42 days after the surgery revealed the loss of the tibiotarsal ratio, decreased radiopacity of the carpal bones, edema, inflammation, muscle atrophy, and the rotation of the anatomical axis of the metatarsals and phalanges. It was then decided to perform a second orthopedic surgery, in which a bone graft and arthrodesis with an 8-screw titanium plate were performed. Four of the plate screws were placed proximally to the tibia and four distally to the tarsus in addition to a screw and a pin transfixed through the distal metaphyseal region of the tibia and calcaneus. Traction was felt during suturing in the dermis and epidermis of the limb, suggesting the possibility of dehiscence of the stitches. After 3 h of surgery, the limb was cold and edematous. After 5 days, the tissue of the medial region of the tarsus developed necrosis, exposing a wound 6 cm long, 1.5 cm wide, and 2 mm deep (wound 1). Another necrotic lesion was observed in the dorsal region of the tarsos - 6.5 cm long, 2 cm wide, 3 mm deep, and exposing 5 cm of the titanium plate (wound 2). Therapy with GaAlAs laser was then performed over the entire length of the wounds. In each therapeutic session, the laser was first used in punctual mode at 10 J/cm², 830 nm, 20 s at each point of the length of the lesion and then in scan mode at 10 J/cm², 830 nm, 1000 Hz, and 40 s continuously. Laser therapy sessions were conducted at 2-4 day intervals, with a 12-day interval between the eighth and ninth sessions. During the entire treatment, cleansing and debridement of the wounds were performed every 48 h with saline and chlorhexidine digluconate, using a compression bandage, as described earlier. Wound 1 healed completely after two laser therapy sessions. In wound 2, tissue repair stagnated after the ninth and last laser session, leaving 4 cm of the titanium plate still exposed. The limb could not support the body weight of the patient, and radiographic examination revealed that the anatomical axis of the metatarsals and the phalanges was rotated and bone conformation was poor. Given the anatomical conditions associated with the patient's clinical picture, it was decided to amputate the limb between the femur and tibia. Discussion: Therapeutic lasers act on mitochondrial respiration, increasing respiratory metabolism and stimulating DNA synthesis and tissue proliferation. In the present case, there was a decrease in inflammatory cells, edema, and the size of the wounds. The pathological conditions of the affected site, influence of trauma, and degree of tissue damage affected the results of the laser therapy. Despite the size difference between wounds 1 and 2, the exposed titanium plate was a determining factor for the partial healing of wound 2.

Effects of glutamine and glutamate on nursery piglets fed diets with different digestible lysine content / Efeitos da glutamina e do glutamato em leitões alimentados com dietas com diferentes níveis de lisina digestível

The objective of the study was to evaluate the effects of glutamine and glutamate (Gln/Glu) on the growth performance and immune response of nursery pigs fed different digestible lysine content. Two hundred and sixteen piglets, weaned at 21 days old, were assigned to a randomized block design according to their initial body weight (BW), in a 2 × 2 factorial arrangement with two levels of lysine (control-lys and low-lys) and two levels of Gln/Glu (0 and 12 g kg-1), with nine replicates. At 26d, piglets consuming the low-lys diet not supplemented with Gln/Glu presented a higher (P < 0.01) incidence of diarrhea than the other treatments. From 21 to 32 d of age, the piglets fed the control-lys diets performed better than those fed low-lys diets (P < 0.01). From 21 to 42 d of age, there was a correlation (P < 0.01) between lysine level and Gln/Glu supplementation for average daily feed intake (ADFI) and feed conversion (FC). Gln/Glu supplementation improved (P < 0.05) the ADFI of pigs fed the low-lys diets, resulting in a higher (P < 0.01) average daily weight gain (ADG) and BW; however, worse (P < 0.05) FC. Piglets consuming control-lys diets had higher (P < 0.05) serum urea nitrogen concentration (SUN) and IgG than low-lys piglets. In addition, Gln/Glu supplementation correlated with higher (P < 0.01) SUN. Dietary supplementation of glutamine and glutamate improved the growth performance of weaned piglets from 21 to 42 days of age, regardless of the diets' lysine levels. In addition, reducing lysine levels 10% below the requirement negatively affects the growth performance and the immune response of nursery piglets.(AU)

O objetivo do estudo foi avaliar os efeitos da glutamina e do glutamato (Gln/Glu) no desempenho e na resposta imune de leitões alimentados com diferentes níveis de lisina digestível. Duzentos e dezesseis suínos, desmamados aos 21 dias de idade, foram distribuídos em um delineamento de blocos ao acaso de acordo com seu peso corporal (PC) inicial, em um arranjo fatorial 2 × 2 com dois níveis de lisina (baixalis e controle-lis) e dois níveis de Gln/Glu (0 e 12 g kg-1), com nove repetições. Aos 26 dias, os leitões que consumiram o baixa-lis não suplementado com Gln/Glu apresentaram maior (P < 0,01) incidência de diarreia quando comparados aos demais tratamentos. Dentre 21 a 32 dias de idade, os leitões alimentados com dietas controle-lis tiveram melhor desempenho do que aqueles alimentados com dietas baixa-lis (P < 0,01). Dos 21 aos 42 dias de idade, houve correlação (P < 0,01) entre o nível de lis e a suplementação com Gln/ Glu para consumo de ração médio diário (CRM) e conversão alimentar (CA). A suplementação com Gln/ Glu melhorou o CRM dos animais alimentados com dietas de baixo teor de lis, resultando em maior ganho de peso médio diário (GMD) e PC; no entanto, a CA piorou. Os leitões que consumiram dietas controle-lis apresentaram maior concentração sérica de nitrogênio ureico (SUN) (P < 0,05) e IgG (P < 0,05) do que os que consumiram dietas baixa-lis. Além disso, os suplementados com Gln/Glu apresentaram maior (P < 0,01) SUN do que os não suplementados. A suplementação dietética de glutamina e glutamato melhorou o desempenho de crescimento de leitões desmamados de 21 a 42 dias de idade, independentemente dos níveis de lis da dieta. Além disso, a redução dos níveis de lis 10% abaixo do requisito afeta negativamente o desempenho do crescimento e a resposta imunológica dos leitões em creche.(AU)

Dietary glutamine-glutamate supplementation enhances growth performance and intestinal villi development in cage-farmed Nile tilapia fingerlings

The objective of this research was to evaluate the effect of increasing levels of associated glutamine and glutamic acid on growth performance and intestinal development of Nile tilapia, Oreochromis niloticus, fingerlings. Five isoproteic (~344.70 g kg−1 crude protein) and isocaloric diets (~3,925 kcal kg−1 gross energy) were developed containing 0, 5, 10, 15, or 20 g kg−1 of associated glutamine and glutamic acid in extruded diets. Fish (n = 2,000, mean body weight of 2.12±0.53 g) were distributed into twenty 1-m3 floating net cages in an entirely randomized design with five treatments and four replicates, and each replicate comprised one floating net cage with 100 fish. Fish were hand-fed seven days per week, three times a day until apparent satiety for 45 days. There was a quadratic effect on final body weight, body weight gain, daily weight gain, feed conversion ratio, protein retention efficiency, net protein utilization, and intestinal villi height with optimized values for supplementation of associated glutamine and glutamic acid at 10.77, 10.67, 10.00, 8.85, 9.85, 10.15, and 10.98 g kg−1, respectively. There was no effect of associated glutamine and glutamic acid supplementation on feed intake, survival, and body composition. We conclude that 10.67 g kg−1 of associated glutamine and glutamic acid is adequate for growth performance optimization, and supplementation at 10.98 g kg−1 exerts trophic action and improves intestinal morphometry in cage-farmed Nile tilapia fingerlings.