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1.
Acta Vet. Brasilica ; 14(2): 115-120, jun. 2020. tab
Artigo em Inglês | VETINDEX | ID: biblio-1453215

Resumo

This study aimed to analyze the microbiological quality of broiler marketed in Teresina, PI. The study was performed in the from September to November 2018, to which three groups of broiler carcasses produced in the city of Teresina were analyzed: without refrigeration, labeling and inspection (n=8); originated from local production, refrigerated, labeled, and inspected (n=8); and originated from other states, frozen, packed, labeled, and inspected (n=8). The collected samples were analyzed regarding the determination of the Most Probable Number (NMP) of total and thermotolerant coliforms, Escherichia coli, and detection of Salmonella spp., besides the measurement of the temperature of the carcasses. The logarithmic means of thermotolerant and total coliforms in the broiler carcasses varied from 0.70 to 4.66 NMP/g and from 0.73 to 4.66 NMP/g, respectively. The bacteria E. coli and Salmonella spp. were also detected in samples with refrigeration and inspection (25 % and 12.5 %, respectively). As for the marketing temperature, only the broiler carcass samples which were chilled and frozen were within the standard of the Brazilian legislation. The results observed in this study indicate the need for improvement in the processing, handling, and storage of the broiler meat marketed in Teresina, PI.


Objetivou-se analisar a qualidade microbiológica de carcaças de frangos comercializadas em Teresina, PI. O estudo foi realizado no período de setembro a novembro de 2018, para o qual foram analisadas carcaças de frango produzidas no município de Teresina, sem refrigeração, rotulagem e inspeção (n=8); de produção local refrigeradas, embaladas com rotulagem e inspecionadas (n=8); e oriundas de outros estados congeladas, embaladas com rotulagem e inspecionadas (n=8). As amostras coletadas foram analisadas quanto à determinação do Número Mais Provável (NMP) de coliformes totais e termotolerantes, Escherichia colie pesquisa de Salmonellaspp., além de aferição da temperatura das carcaças. As médias logarítmicas de coliformes termotolerantes e totais nas carcaçasde frango analisadas variaram de 0,70 a 4,66 NMP/g e de 0,73 a 4,66 NMP/g, respectivamente. Também foram encontradas bactérias E. colie Salmonellaspp. em amostras com refrigeração e com inspeção (25 % e 12,5 %, respectivamente). Quanto à temperatura de comercialização apenas as amostras de carcaça de frango resfriadas e as congeladas mostraram-se dentro do padrão da legislação brasileira. Os resultados observados nesse estudo indicam a necessidade de melhoria no processamento, manipulação e armazenamentoda carne de frango comercializado em Teresina, PI.


Assuntos
Animais , Enterobacteriaceae/classificação , Escherichia coli , Galinhas/microbiologia , Higiene dos Alimentos , Salmonella , Comércio
2.
Acta Vet. bras. ; 14(2): 115-120, jun. 2020. tab
Artigo em Inglês | VETINDEX | ID: vti-27006

Resumo

This study aimed to analyze the microbiological quality of broiler marketed in Teresina, PI. The study was performed in the from September to November 2018, to which three groups of broiler carcasses produced in the city of Teresina were analyzed: without refrigeration, labeling and inspection (n=8); originated from local production, refrigerated, labeled, and inspected (n=8); and originated from other states, frozen, packed, labeled, and inspected (n=8). The collected samples were analyzed regarding the determination of the Most Probable Number (NMP) of total and thermotolerant coliforms, Escherichia coli, and detection of Salmonella spp., besides the measurement of the temperature of the carcasses. The logarithmic means of thermotolerant and total coliforms in the broiler carcasses varied from 0.70 to 4.66 NMP/g and from 0.73 to 4.66 NMP/g, respectively. The bacteria E. coli and Salmonella spp. were also detected in samples with refrigeration and inspection (25 % and 12.5 %, respectively). As for the marketing temperature, only the broiler carcass samples which were chilled and frozen were within the standard of the Brazilian legislation. The results observed in this study indicate the need for improvement in the processing, handling, and storage of the broiler meat marketed in Teresina, PI.(AU)


Objetivou-se analisar a qualidade microbiológica de carcaças de frangos comercializadas em Teresina, PI. O estudo foi realizado no período de setembro a novembro de 2018, para o qual foram analisadas carcaças de frango produzidas no município de Teresina, sem refrigeração, rotulagem e inspeção (n=8); de produção local refrigeradas, embaladas com rotulagem e inspecionadas (n=8); e oriundas de outros estados congeladas, embaladas com rotulagem e inspecionadas (n=8). As amostras coletadas foram analisadas quanto à determinação do Número Mais Provável (NMP) de coliformes totais e termotolerantes, Escherichia colie pesquisa de Salmonellaspp., além de aferição da temperatura das carcaças. As médias logarítmicas de coliformes termotolerantes e totais nas carcaçasde frango analisadas variaram de 0,70 a 4,66 NMP/g e de 0,73 a 4,66 NMP/g, respectivamente. Também foram encontradas bactérias E. colie Salmonellaspp. em amostras com refrigeração e com inspeção (25 % e 12,5 %, respectivamente). Quanto à temperatura de comercialização apenas as amostras de carcaça de frango resfriadas e as congeladas mostraram-se dentro do padrão da legislação brasileira. Os resultados observados nesse estudo indicam a necessidade de melhoria no processamento, manipulação e armazenamentoda carne de frango comercializado em Teresina, PI.(AU)


Assuntos
Animais , Galinhas/microbiologia , Enterobacteriaceae/classificação , Higiene dos Alimentos , Salmonella , Escherichia coli , Comércio
3.
Ci. Rural ; 48(2): e20161034, 2018. tab
Artigo em Inglês | VETINDEX | ID: vti-18724

Resumo

In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.(AU)


Com o objetivo de detectar e identificar Campylobacter spp. em carcaças de frango de corte utilizando três metodologias distintas - ensaio imunoenzimático VIDAS®30, Reação em Cadeia da Polimerase (PCR) e PCR em tempo real - foram coletadas e analisadas 43 carcaças de frango resfriadas e 43 congeladas. Quatro carcaças refrigeradas (4,6%) foram consideradas positivas para Campylobacter spp. pelo VIDAS®30 e nenhuma amostra positiva foi identificada quando utilizada a técnica de PCR. Porém, ao analisar as carcaças pela metodologia da PCR em tempo real, foi observada uma maior incidência de Campylobacter spp., com 45 amostras (52,3%) positivas, sendo que Campylobacter jejuni foi a espécie mais frequentemente encontrada nas amostras (88,8%). Não foi observada diferença na frequência do micro-organismo entre carcaças de frangos resfriadas e congeladas. Concluiu-se que a técnica de PCR em tempo real apresentou maior sensibilidade na detecção de Campylobacter spp. em carcaças de frangos de corte e que foi encontrada elevada presença de carcaças contaminadas, especialmente por C. jejuni.(AU)


Assuntos
Campylobacter/isolamento & purificação , Carne/microbiologia , Galinhas/microbiologia , Reação em Cadeia da Polimerase em Tempo Real , Técnicas Imunoenzimáticas
4.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1457715

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

5.
Acta sci. vet. (Impr.) ; 45: 1-6, 2017. tab
Artigo em Português | VETINDEX | ID: biblio-1457627

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome. Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples analyzed, the frequency of isolation of thermophilic Campylobacter was 1.82% (8/440) in microregion 1, 4.95% (10/202) in microregion 2 and 13.86% (23/166) in the microregion 3, totaling 5.07% of positive samples (41/808). Comparing the microregions, it was verified that there was no significant difference (P > 0.05) between the isolation frequencies of microregions 1 and 2. However, there was a significant difference (P < 0.05) between the isolation rates of Microregion 3 in relation to microregions 1 and 2.[...]


Assuntos
Animais , Campylobacter/isolamento & purificação , Carne/microbiologia , Galinhas/microbiologia , Infecções por Campylobacter/veterinária , Inocuidade dos Alimentos
6.
Acta sci. vet. (Online) ; 45: 1-6, 2017. tab
Artigo em Português | VETINDEX | ID: vti-20297

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome. Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples analyzed, the frequency of isolation of thermophilic Campylobacter was 1.82% (8/440) in microregion 1, 4.95% (10/202) in microregion 2 and 13.86% (23/166) in the microregion 3, totaling 5.07% of positive samples (41/808). Comparing the microregions, it was verified that there was no significant difference (P > 0.05) between the isolation frequencies of microregions 1 and 2. However, there was a significant difference (P < 0.05) between the isolation rates of Microregion 3 in relation to microregions 1 and 2.[...](AU)


Assuntos
Animais , Campylobacter/isolamento & purificação , Galinhas/microbiologia , Carne/microbiologia , Infecções por Campylobacter/veterinária , Inocuidade dos Alimentos
7.
R. Inst. Adolfo Lutz ; 73(4): 372-376, 2014. tab
Artigo em Português | VETINDEX | ID: vti-715211

Resumo

The direct method for detecting and enumerating Campylobacter spp. in chicken meat is easy to perform, but the volumes of 100μL and 400μL, as recommended in some methodologies, are often unable to achieve the colonies counts due to the occurrence of confluent growth or microbial contaminants. This study aimed at evaluating the different volumes for rinsing chicken meats in order to minimize the interference of microbial contaminants, without compromising the sensitivity of direct methodology. Rinse volumes of 5, 10, 50, 100 and 400μL were tested using the modified charcoal cefoperazone deoxycholate agar (mCCDA) and modified Bolton agar. The presence of Campylobacter was confirmed by phenotypic methods and PCR assay. However, the strategy of using less than 100 µL of rinse volumes did not improve the isolation and enumeration of Campylobacter colonies, because it reduces the sensitivity of the assay. Thus, a possible solution to minimize the interference of microbial contaminants would be in developing new selective media or incorporate them into other existing antimicrobial drugs.(AU)


O método direto de detecção e contagem de Campylobacter spp. em carne de frango é de fácil execução, porém os volumes de 100 µL e de 400 µL, preconizados em algumas metodologias, muitas vezes impossibilitam a contagem de colônias pela ocorrência de crescimento confluente ou de microbiota contaminante. O objetivo do presente estudo foi de avaliar os diferentes volumes de enxaguadura de carne de frango com a finalidade de minimizar a interferência da microbiota contaminante, sem comprometer a sensibilidade do método. Os volumes de enxaguadura de 5, 10, 50, 100 e 400 µL foram testados utilizando-se os meios seletivo diferenciais ágar carvão cefoperazona desoxicolato modificado (mCCDA) e ágar Bolton modificado. A presença de Campylobacter spp. foi confirmada por métodos fenotípicos e por PCR. No entanto, a estratégia de utilização de volumes menores do que 100 µL de enxaguadura não melhoraram o isolamento e a contagem de colônias de Campylobacter, porque houve diminuição da sensibilidade do ensaio. A provável solução para minimizar a interferência da microbiota contaminante seria desenvolver novos meios seletivos ou incorporá-los aos antimicrobianos já existentes.(AU)


Assuntos
Campylobacter/isolamento & purificação , Carne/microbiologia , Alimentos Resfriados , Carga Bacteriana , Galinhas
8.
Artigo em Português | LILACS-Express | VETINDEX | ID: biblio-1492981

Resumo

The aim of this study was to search Salmonella spp. occurrence in broiler poultry shed and poultry products from slaughterhouse in central county of Mato Grosso do Sul. Were analyzed 134 drag swabs in broiler poultry farms located in five counties 123 samples of chickens carcasses, viscera and chilled water from the slaughterhouse. The results showed that 11.28% of 257 samples tested positive for Salmonella, and 1.95% from the field and 9.33% from the slaughterhouse. The serovars found were Salmonella Enteritidis (1.16%), S. Typhimurium (1.94%), S. Senftenberg (0.77%), S. Schwarzengrund (4.28%), S. Livingstone (0.38%), S. Corvallis (1.55%) and Salmonella enterica subspecies enterica (O: 4.5:-:1.2) with 1.16%. The use of two enrichment broths and two or more plating means increases the chances of Salmonella spp. Isolation. The serovars Enteritidis and Typhimurium, important in the poultry industry, were found in field and slaughterhouse. It can be concluded that there is an occurrence of different serovars of Salmonella spp. In poultry slaughterhouse and in Mato Grosso do Sul central county. Serovar Schwarzengrum shows the highest percentage of occurrence in the slaughterhouse, suggesting that further studies are conducted to determine the importance of presence compared to other serovars. Control programs and risk analysis are essential for maintaining the health of poultry and


Objetivou-se com este estudo pesquisar a ocorrência de Salmonella spp. em aviários de frangos de corte e em produtos de abatedouro avícola na região central de Mato Grosso do Sul. Foram analisados 134 suabes de arraste em aviários de frangos de corte localizados em cinco municípios e 123 amostras de carcaças de frango, vísceras e água do chiller provenientes do abatedouro. Os resultados demonstraram que 11,28% das 257 amostras apresentaram resultados positivos para Salmonella, dos quais 1,95% provenientes do campo e 9,33% do abatedouro. Os sorovares encontrados foram: S. Enteritidis (1,16%), S. Typhimurium (1,94%), S. Senftenberg (0,77%), S. Schwarzengrund (4,28%), S. Livingstone (0,38%), S. Corvallis (1,55%) e Salmonella enterica subspécie enterica (O:4,5:-:1,2) com 1,16%. A utilização de dois caldos de enriquecimento e de dois ou mais meios de plaqueamento aumenta as chances de isolamento de Salmonella spp. Os sorovares Enteriditis e Typhimurium, importantes na indústria avícola, foram encontrados no campo e no abatedouro. Pode-se concluir que há ocorrência de diferentes sorovares de Salmonella spp. nos aviários e no abatedouro da região central de Mato Grosso do Sul. O sorovar Schwarzengrund apresentou maior percentual de ocorrência no abatedouro, o que sugere a realização de estudos mais aprofundados que possam determinar a importância da sua presença frente aos demais sor

9.
Tese em Português | VETTESES | ID: vtt-201844

Resumo

As doenças de transmissão alimentar (DTA) por bactérias constituem um problema de saúde pública no mundo. O objetivo do trabalho foi identificar por métodos microbiológicos e moleculares a presença de Campylobacter spp., Salmonella spp. e Escherichia coli em frangos de Botucatu-SP, Brasil. Sessenta (60) amostras de frangos resfriados de diferentes marcas foram coletadas aleatoriamente em diferentes estabelecimentos localizados tanto na periferia, quanto no centro da cidade de Botucatu, SP, no período de julho a outubro de 2015. Posteriormente, Campylobacter spp, Salmonella spp e E. coli foram isolados através de métodos microbiológicos convencionais. A confirmação das bactérias isoladas se realizou por provas bioquímicas e PCR. Além disso, as provas para determinar a resistência antimicrobiana e a existência de gene integron classe 1 foram realizadas. Os resultados demostraram a presença de três das principais bactérias que causam doenças de origem alimentar em carcaças de frango provenientes de supermercados e casas de carne de Botucatu. A prevalência de Campylobacter, Salmonella, e E. coli foi 38,3%, 13,3% e 60%, respectivamente. A resistência antimicrobiana dos isolados foi elevada e presença do gene íntegron classe 1 foi identificada em alguns agentes patogénicos.


The transmission of Foodborne Diseases (FBD) by bacteria constitutes a public health problem in the world. The objective of this study was to identify by microbiological and molecular methods the presence of Campylobacter spp., Salmonella spp. and Escherichia coli in broiler chickens at Botucatu, SP, Brazil. Sixty samples of chilled chickens of different brands were randomly collected from supermarkets and meat houses located in both the periphery and the center of the city, in the period from July to October 2015. Later, Campylobacter spp., Salmonella spp., and E. coli were isolated by conventional microbiological methods. Confirmation of the isolated bacteria was performed by biochemical and PCR tests. In addition, proofs to determine antimicrobial resistance and the existence of class 1 integron gene were carried out. Results confirmed the presence of the three assessed bacteria in poultry carcasses from selected establishments. The prevalence was 38.3%, 13.3% and 60% to Campylobacter, Salmonella and E. coli, respectively. Antimicrobial resistance of the isolates was high and the presence of integron class 1 gene has been identified in some pathogens.

10.
Tese em Português | VETTESES | ID: vtt-204036

Resumo

BORTOLI, William. Ocorrência de Campylobacter termofílicos em carcaças resfriadas de frangos abatidos na região oeste de Santa Catarina. 2016. 85 f. Dissertação (Mestrado em Ciência Animal - Área: Saúde Animal) - Universidade do Estado de Santa Catarina. Programa de Pós - Graduação em Ciência Animal, Lages, 2016. Dentre os patógenos veiculados por alimentos, estão as bactérias termofílicas do gênero Campylobacter, importantes agentes de gastrenterite de origem alimentar, neste caso denominada de campilobacteriose. Campylobacter são definidos como bastonetes Gram-negativos, curvos ou espiralados, capnófilos, com movimento característico de saca-rolha ou em vai e vem. Destacam-se como consideráveis fontes de transmissão de Campylobacter termofílicos para o homem a carne e miúdos de frango, contaminadas durante a manipulação e operações de abate mal conduzidas, e a contaminação cruzada destes com outros alimentos que serão ingeridos crus. Devido ao destaque que a região oeste de Santa Catarina possui na produção e comercialização da carne de frango, fez-se necessário verificar a ocorrência do patógeno em carcaças resfriadas de frangos abatidos nesta região, e seu comportamento frente às estações do ano. Por um período de 26 meses, a partir de janeiro de 2013, foram semanalmente coletadas amostras de carcaça de frango, após o processo de resfriamento em água, em abatedouros sob Inspeção Federal das três maiores microrregiões em número de abate de frangos da região oeste de Santa Catarina, totalizando 808 amostras. Foi realizada a pesquisa de Campylobacter termofílicos conforme a metodologia recomendada pela ISO 10272-1:2006. Campylobacter termofílicos foram isolados em 1,82% (8/440) das amostras da microrregião 1, em 4,95% (10/202) das amostras da microrregião 2 e em 13,86% (23/166) amostras da microrregião 3, totalizando 41 amostras positivas (5,07%) do total de amostras coletadas. O índice relatado para a microrregião 3 foi estatisticamente significativo (P<0,05) quando comparado aos índices encontrados para as outras duas microrregiões. Embora as taxas encontradas tenham sidas abaixo das esperadas quando comparadas com as já publicadas para esta região, ainda fornecem riscos aos consumidores, principalmente por meio da contaminação cruzada com alimentos que serão consumidos crús, sendo necessários controles maiores na cadeia produtiva e abate dos frangos. Com relação às estações do ano, os índices de Campylobacter termofílicos não foram estatisticamente significativos (P<0,05), porém, faz-se necessário uma avaliação por um período maior de tempo, já que houve uma tendência numericamente positiva com relação ao verão.


Among the pathogens foodborne are thermophilic bacteria of genus Campylobacter, gastroenteritis important agents of food-borne, in this case called campylobacteriosis. Campylobacter are defined as Gram-negative, curved or spiral, capnófilos with characteristic movement of corkscrew or comes and goes. They stand out as considerable thermophilic Campylobacter transmission sources for man meat and chicken giblets contaminated during handling and misguided slaughter operations, and cross-contamination of these with other foods that will be eaten raw. Due to the emphasis that the western region of Santa Catarina has the production and marketing of chicken, it was necessary to verify the occurrence of the pathogen in chilled carcasses of chickens slaughtered in this region, and its behavior to the seasons. For a period of 26 months, from January 2013, chicken carcass samples were weekly collected after the cooling process water in slaughterhouses under Federal Inspection of the three largest micro-regions in broiler slaughter number of the western region of Santa Catarina, totaling 808 samples. Campylobacter thermophiles was performed according to the methodology recommended by ISO 10272-1: 2006. Thermophilic Campylobacter were isolated in 1.82% (8/440) of the micro-region 1 of the samples, 4.95% (10/202) of samples of themicro-region 2 and 13.86% (23/166) samples of the micro-region 3 totaling 41 positive samples (5.07%) of the total samples collected. The index reported for the microregion 3 was statistically significant (P <0.05) when compared to the ratios found for the other two microregions. Although the prevalences have solid lower than expected when compared to the already published for this region, still provide risk to consumers, mainly through cross-contamination with food that will be consumed raw state, requiring greater controls on the production chain and slaughter of chickens. With respect to the seasons, the Campylobacter rates thermophilic were not statistically significant (P <0.05), however, it is necessary to evaluate for a longer period of time, since there was a numerically positive trend with respect to summer.

11.
Tese em Português | VETTESES | ID: vtt-200591

Resumo

Apesar dos avanços nos processos de produção e comercialização de frango de corte, o controle, a redução e o monitoramento de agentes associados a doenças transmissíveis por alimentos (DTAs) ainda é um desafio. Neste sentido, este trabalho foi desenvolvido com o objetivo de determinar a ocorrência de Salmonella enterica e Staphylococcus aureus resistente à meticilina (MRSA) em carcaças de frango comercializadas no Estado da Paraíba. Com base no cálculo do tamanho amostral, utilizou-se 138 carcaças de frangos para a pesquisa de Salmonella enterica e para Staphylococcus aureus resistente à meticilina (MRSA), utilizou-se 60 carcaças de frangos. As carcaças de frango foram obtidas em estabelecimentos comerciais varejistas e estavam disponíveis nas formas in natura, resfriada e congelada. Após o isolamento microbiológico convencional e realização de testes bioquímicos, S. enterica estava presente em 17,8% (24/138) das carcaças estudadas, sendo em 16,3% (8/49) das carcaças in natura, 15,9% (6/38) das carcaças resfriadas e 19,6% (10/51) das carcaças congeladas. Para identificação do S. aureus, foi realizada PCR para identificação de marcadores moleculares específicos. Dentre as amostras analisadas, 33,33% (20/60) foram positivos para Staphylococcus aureus. A prevalência desta bactéria foi observada apenas nas carcaças in natura 15% (3/20) e congeladas 53,1% (17/32). De todos os S. aureus isolados, 8,3% (4/60) apresentaram resistência à meticilina (MRSA). As estirpes de Salmonella enterica foram mais resistentes à enrofloxacina (72,1%), tetraciclina (30,2%), cefalotina (27,9%), ampicilina (23,3%), amoxicilina (13,95%), sulfametoxazol + trimetoprim (11,63%) e norfloxacina (4,65%). Enquanto que as estirpes de S. aureus apresentaram resistência mais frequentes à penicilina (60,4%), ampicilina (47,9%) e azitromicina (41,7%), seguidos de tetraciclina (37,5%) e clindamicina (22,9%). As estirpes caracterizadas fenotipicamente e genotipicamente como MRSA apresentaram resistência não apenas aos -lactâmicos, mas também a outras classes de antimicrobianos. A análise de similaridade genética por PFGE e Rep-PCR indicou que determinadas linhagens de Salmonella enterica são comuns a diferentes sistemas de produção. E que a combinação desses dois métodos genotípicos PFGE e Rep-PCR pode aumentar a capacidade de discriminação, principalmente para a tipificação de MRSA.


Despite advances in production processes and broiler marketing, control, reduction and monitoring agents associated with diseases transmitted by food (DTAs) is still a challenge. Thus, this study was conducted in order to determine the occurrence of Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) in chicken carcasses commercialized in the state of Paraiba. Based on the sample size calculation, we used 138 chicken carcasses for Salmonella enterica research and methicillin-resistant Staphylococcus aureus (MRSA), used 60 chicken carcasses. The chicken carcasses were obtained from retail shops and forms were available in fresh, chilled and frozen. After conventional microbiological isolation and performing biochemical tests, S. enterica was present in 17.8% (24/138) of carcasses under study, and in 16.3% (8/49) of carcasses in nature, 15.9% (6/38) of chilled carcasses and 19.6% (10/51) of frozen carcasses. For S. aureus identification, PCR was performed for identification of specific molecular markers. Among the samples analyzed, 33.33% (20/60) were positive for Staphylococcus aureus. The prevalence of these bacteria was observed only in the carcasses in natura 15% (3/20) and frozen 53.1% (17/32). Of all S. aureus isolates, 8.3% (4/60) were resistant to methicillin (MRSA). Salmonella enterica strains were more resistant to enrofloxacin (72.1%), tetracycline (30.2%), cephalothin (27.9%), ampicillin (23.3%), amoxicillin (13.95%), sulfamethoxazole trimethoprim (11.63%) and norfloxacin (4.65%). While the S. aureus strains had more frequent resistance to penicillin (60.4%), ampicillin (47.9%) and azithromycin (41.7%), followed by tetracycline (37.5%), clindamycin (22.9%). Strains genotypically and phenotypically characterized as MRSA were resistant not only to -lactams, but also to other classes of antimicrobial agents. The genetic similarity analysis by PFGE and Rep-PCR indicated that certain Salmonella enterica strains are common to different production systems. And the combination of these two PFGE genotypic methods and Rep-PCR can increase the capacity of discrimination, especially for the characterization of MRSA.

12.
Tese em Português | VETTESES | ID: vtt-200783

Resumo

PODODERMATITE, LESÕES DE CARCAÇA E RESISTÊNCIA DA PELE DE FRANGOS DE CORTE SUPLEMENTADOS COM MINERAIS ORGÂNICOS Com objetivo de avaliar o efeito da suplementação dos microminerais orgânicos zinco e selênio na dieta de frangos de corte, sobre a incidência e caracterização de pododermatite, lesão de carcaça, resistência da pele, desempenho e rendimento de carcaça e partes. Para isto, utilizou-se 1260 pintos de um dia de idade, machos, da linhagem Cobb® 500. O programa alimentar foi dividido em quatro fases e o delineamento experimental adotado foi o inteiramente casualisado em esquema fatorial 3x2+1, sendo três níveis de suplementação de zinco (Zn) orgânico (0, 45, 90 mg/kg de zinco), dois níveis de suplementação de selênio (Se) orgânico (0, 0,3 mg/kg de selênio-levedura) e uma dieta controle com 0,3 mg/kg de Se e 60 mg/kg de Zn inorgânicos, com seis repetições de 30 aves cada. Aos 41 dias de idade todas as aves foram avaliadas no aviário quanto a incidência de pododermatite. Aos 42 dias de idade 588 aves (84 aves/tratamento) foram abatidas no abatedouro experimental da FMVZ, UNESP. Na ocasião foram avaliados incidência de lesões de carcaça, resistência da pele, avaliação histológica do coxim plantar e pele, rendimento de carcaça e partes e desempenho das aves. Para pododermatite foi observada diferença (P0,05) na pata esquerda, entre os escores de lesão 0 e 2, sendo que as que receberam maior suplementação de zinco apresentaram menor incidência. Para lesões de carcaça houve diferença (P0,05) com relação à percentagem de hematomas e contusões de asa. Aliando-se os resultados de desempenho, rendimento de carcaça e partes e as avaliações histológicas do coxim plantar e pele, os melhores resultados foram obtidos para aves que receberam 0,3 mg/kg de Se e 45 ou 90 mg/kg de zinco orgânicos. A utilização de selênio e zinco orgânico não influenciou a incidência de pododermatite e de lesões de carcaça. Os resultados obtidos pelo uso de selênio e zinco orgânicos em menor quantidade que a fonte inorgânica não afetaram o desempenho, o rendimento de carcaça e partes e ainda, melhoram a estrutura histológica da região da pododermatite. Suplementação de minerais orgânicos e vitamina E na identificação microbiológica de pododermatite, rendimento de carcaça, qualidade da carne e sistema imune de frangos de corte Um experimento foi realizado com objetivo de avaliar a suplementação dos minerais orgânicos (selênio e zinco) aliados a vitamina E sobre a incidência e caracterização microbiológica da pododermatite, qualidade de carcaça e da carne e sobre o sistema imune de frangos de corte. Para isto, utilizou-se 1260 pintos de um dia de idade, machos, da linhagem Cobb® 500. O programa alimentar foi dividido em quatro fases e o delineamento experimental adotado foi o inteiramente casualisado em esquema fatorial 3x2+1, sendo três níveis de suplementação de vitamina E (100, 150 e 200UI/kg de ração) e dois níveis de suplementação de microminerais inorgânico ou orgânico (controle- 0,3 mg/kg Se + 60 mg/kg de Zn inorgânicos ou 0,3 mg/kg de Se e 45 mg/kg de Zn orgânicos) e uma dieta controle, com seis repetições de 30 aves. Aos 10 dias de idade todas as aves foram vacinadas contra a Doença de Newcastle. Aos 41 dias de idade todas as aves foram avaliadas quanto a incidência de pododermatite. Aos 42 dias, um total de 420 aves foram abatidas no abatedouro experimental para avaliação da incidência de lesões de carcaça, resistência da pele, rendimento de carcaça e partes, qualidade de carne, quantificação microbiológica e histologia do coxim plantar e pele. Houve diferença (P 0,05) para o escore de lesão grave. As aves que receberam dieta com minerais orgânicos e maior quantidade de vitamina E apresentaram menor incidência de pododermatite. Aves que receberam zinco e selênio orgânico apresentaram maior quantidade de colágeno e a epiderme mais espessa (P 0,05). Houve redução da área cortical da Bursa para aves que receberam maior quantidade de vitamina E e fonte orgânica. Conclui-se que, aves que receberam dieta com zinco e selênio orgânico apresentaram menor incidência de pododermatite, aumento da quantidade de colágeno e espessura da epiderme e estão melhor preparadas para reagir frente a um desafio imunológico.


FOOT PAD DERMATITIS, SKIN LESION AND STRENGTH IN BROILER CHICKENS SUPPLEMENTED WITH ORGANIC MINERALS The present study aimed at evaluating the effects of organic zinc and selenium supplementation in the diet of broiler chicken, on the incidence of pododermatitis, skin lesions and skin strength, footpad and skin histology and performance. In total, 1260 one-day-old male Cobb® 500 broilers were housed. The feeding program included four phases and a completely randomized experimental design in a 3x2+1 factorial arrangement, using three organic zinc supplementation levels (0, 45, 90 mg zinc/kg feed), two organic selenium supplementation levels (0 or 0.3 mg selenium yeast/kg feed) and a positive-control diet with inorganic trace mineral supplementation, with six replicates of 30 birds each. At 41 days of age, all birds were submitted to pododermatitis incidence evaluation. At 42 days of age, a total of 588 birds were sacrificed at the FMVZ, UNESP experimental processing plant, and carcasses were evaluated to the incidence of skin lesions, skin strength, footpad dermatitis and performance. Significant difference (P0.05) in the left leg in lesion scores 0 (absent) and 2 (severe lesion) being broilers fed the highest level of zinc supplementation (90 mg/kg) presented the lowest incidence of contact pododermatitis. Related to skin lesions differences were detected (P0.05) just in incidence of carcass bruising and wing bruises. In skin strength no differences were detected related to levels of organic zinc and selenium. Combining the results of performance and histological evaluations of the footpad and skin, the best results were obtained for birds fed 0.3 mg/kg Se and 45 or 90 mg/kg organic zinc. The use of organic selenium and zinc did not influence the incidence of foot pad dermatitis and carcass lesion. The results from use selenium and zinc in low amount compare to inorganic source do not affect the performance, carcass and parts yield, but improve the histological structure of foot pad dermatites. Keywords: carcass quality, complexed mineral, footpad, poultry Organic mineral and vitamin E supplementation on microbiological podermatite contamination, carcass yield, meat quality and immune system of broiler The present study aimed at evaluating the effects of organic zinc and selenium supplementation combined with vitamin E on the incidence of pododermatitis, microbiological characterization, footpad and skin histology, skin lesions and skin strength, meat quality, carcass and cut yield and in immune system of broiler chicken. In total, 1260 one-day-old male Cobb® 500 broilers were housed. The feeding program included four phases and a completely randomized experimental design in a 3x2+1 factorial arrangement was applied, using three vitamin E supplementation levels (100, 150, 200UI/kg feed), two minerals supplementation inorganic or organic (control- minerals in inorganic form or 0.3 mg selenium yeast/kg plus 45 mg/kg organic zinc) and a positive-control diet (vitamin E and minerals in commercial premix), with six replicates of 30 birds each. At 10 days of age, all birds were immunized for Newcastle disease. At 41 days of age, all birds were submitted to footpad examination of both feet in the broiler house to pododermatitis incidence. At 42 days of age, a total of 420 birds, treatment) were sacrificed at the experimental processing plant, the carcasses were evaluated to the incidence of skin lesions; skin strength, carcass and cut yield, and it was collected samples to meat quality analyses, microbiological characterization, histological examination of foot pad and skin. Significant difference (P0.05) in lesion scores 3 (severe), broilers fed with organic mineral and high vitamin E level presented the lowest incidence of contact pododermatitis. The source organic or inorganic affected (P 0,05) and broiler fed with organic zinc and selenium have the collagen layer and epidermis thicker. Birds fed with inorganic source and 150UI of vitamin E had the highest dermatosi incidence. It was concluded that broiler supplemented with organic zinc and selenium reduce the incidence of pododermatitis and increased thickness of collagen and epidermis. Key words: birds, color, foot pad dermatitis, quality, organic mineral COMPLEXED ZINC AND MANGANESE SUPPLEMENTATION ON GROWTH PERFORMANCE FOOT PAD DERMATITIS, CARCASS AND MEAT QUALITY OF BROILERS This study evaluated the influence of dietary complexed zinc (CZn) and manganese (CMn) supplementation on growth performance, the incidence of foot pad dermatitis (FPD), skin quality, the incidence and severity of black bone syndrome (BBS) and myopathies of the breast muscle, processing yield and meat quality of broilers. A total of 1,120 (14 replicates/20 birds each) male broilers were assigned to 4 dietary treatments: 1) Inorganic control diet (IC), 80 ppm of ZnSO4 and 90 ppm of MnSO4; 2) Same as IC, except CZn replaced 40 ppm of Zn from ZnSO4 (IC+CZn-Zn); 3) IC plus additional 40 ppm of CZn (IC+CZn); 4) Same as IC, except CZn and CMn replaced 40 ppm of Zn and Mn (IC+CZn+CMn). Each treatment was provided in a 3- stage feeding program. At 35 and 49 d of age, five birds per pen were processed, chilled overnight, and deboned. The GLM procedures of SAS was used to analyze the data and means were separated by Tukeys Test when significant (P0.05). Broilers reared on IC+CZn and IC+CZn+CMn showed improvement in BW at 28 and 49 d of age when compared to inorganic control diet (IC). The drip loss was lower in birds on IC+CZn-Zn diet. No differences in carcass and component yields were detected because of dietary treatments. The BBS and color (L*, a* and b*) were affected (P0.05) by storage method, where frozen samples exhibited higher incidence of severity of BBS when compared to those stored non-frozen. In conclusion, no differences in FPD, carcass quality and yield was observed due to dietary treatments in this study, except for the improvements in BW and feed conversion ratio. Freezing bone-in carcass portions increased the occurrence of BBS upon cooking as compared to refrigerated portions. Key words: broiler, manganese, quality, skin, zinc

13.
Artigo em Inglês | VETINDEX | ID: vti-733225

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

14.
Artigo em Inglês | VETINDEX | ID: vti-732362

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

15.
Artigo em Inglês | VETINDEX | ID: vti-731339

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

16.
Artigo em Inglês | VETINDEX | ID: vti-730632

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

17.
Artigo em Inglês | VETINDEX | ID: vti-730428

Resumo

Background: The thermophilic bacteria of the genus Campylobacter are important agents of alimentary gastroenteritis, called campylobacteriosis. These microorganisms multiply in temperatures ranging from 25ºC to 46ºC, however, low temperatures are incompatible with their multiplication. For this reason, the seasons of the year may interfere with the level of contamination by Campylobacter sp. The main sources of transmission are contaminated meat and giblets from poultry during poorly conducted slaughter operations. The disease may present itself with a different range of forms of disease: from mild signs of gastrointestinal infection to more severe cases, such as the Guillain-Barré syndrome.Material, Methods & Results: Due to the great importance of western Santa Catarina to the poultry industry, it was necessary to verify the occurrence of the pathogen in cold carcasses of broilers slaughtered in this region, and its variation through the seasons of the year. From January 2013 to February 2015 broiler carcasses were collected weekly, after the water cooling process, in slaughterhouses under Federal Inspection of the three largest microregions of western Santa Catarina in terms of number of broilers slaughtered, totaling 808 samples. The assessment of thermophilic Campylobacter was performed according to the methodology recommended by ISO 10272-1: 2006. Of the 808 samples a

18.
Artigo em Português | VETINDEX | ID: vti-711999

Resumo

The aim of this study was to search Salmonella spp. occurrence in broiler poultry shed and poultry products from slaughterhouse in central county of Mato Grosso do Sul. Were analyzed 134 drag swabs in broiler poultry farms located in five counties 123 samples of chickens carcasses, viscera and chilled water from the slaughterhouse. The results showed that 11.28% of 257 samples tested positive for Salmonella, and 1.95% from the field and 9.33% from the slaughterhouse. The serovars found were Salmonella Enteritidis (1.16%), S. Typhimurium (1.94%), S. Senftenberg (0.77%), S. Schwarzengrund (4.28%), S. Livingstone (0.38%), S. Corvallis (1.55%) and Salmonella enterica subspecies enterica (O: 4.5:-:1.2) with 1.16%. The use of two enrichment broths and two or more plating means increases the chances of Salmonella spp. Isolation. The serovars Enteritidis and Typhimurium, important in the poultry industry, were found in field and slaughterhouse. It can be concluded that there is an occurrence of different serovars of Salmonella spp. In poultry slaughterhouse and in Mato Grosso do Sul central county. Serovar Schwarzengrum shows the highest percentage of occurrence in the slaughterhouse, suggesting that further studies are conducted to determine the importance of presence compared to other serovars. Control programs and risk analysis are essential for maintaining the health of poultry and


Objetivou-se com este estudo pesquisar a ocorrência de Salmonella spp. em aviários de frangos de corte e em produtos de abatedouro avícola na região central de Mato Grosso do Sul. Foram analisados 134 suabes de arraste em aviários de frangos de corte localizados em cinco municípios e 123 amostras de carcaças de frango, vísceras e água do chiller provenientes do abatedouro. Os resultados demonstraram que 11,28% das 257 amostras apresentaram resultados positivos para Salmonella, dos quais 1,95% provenientes do campo e 9,33% do abatedouro. Os sorovares encontrados foram: S. Enteritidis (1,16%), S. Typhimurium (1,94%), S. Senftenberg (0,77%), S. Schwarzengrund (4,28%), S. Livingstone (0,38%), S. Corvallis (1,55%) e Salmonella enterica subspécie enterica (O:4,5:-:1,2) com 1,16%. A utilização de dois caldos de enriquecimento e de dois ou mais meios de plaqueamento aumenta as chances de isolamento de Salmonella spp. Os sorovares Enteriditis e Typhimurium, importantes na indústria avícola, foram encontrados no campo e no abatedouro. Pode-se concluir que há ocorrência de diferentes sorovares de Salmonella spp. nos aviários e no abatedouro da região central de Mato Grosso do Sul. O sorovar Schwarzengrund apresentou maior percentual de ocorrência no abatedouro, o que sugere a realização de estudos mais aprofundados que possam determinar a importância da sua presença frente aos demais sor

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