Evaluation of the antinociceptive effect generated by citronellal monoterpene isomers / Avaliação do efeito antinociceptivo gerado por isômeros do monoterpeno citronelal

Due to the complex nature of pain and the participation of physical, cognitive, psychological and behavioral aspects, pain management has several approaches. The use of medicinal plants in developing countries is quite expressive. Seeking new options for the treatment of emerging or debilitating diseases. Therefore, the present study seeks to elucidate the effects of the monoterpene, citronellal, differentiating its activity by isomers (R)-(+) and (S)-(-) citronellal. The study used several methods to evaluate the effects of citronellal isomers on motor coordination, nociceptive response, and the involvement of opioid, glutamatergic, and transient receptor pathways. The methods included rota-rod, hot-plate, and formalin tests, as well as the use of specific inhibitors and agonists. Data were analyzed using inferential statistics with a 95% confidence level. Both isomers did not significantly affect the motor coordination of the studied animals. The isomer (S)-(-) citronellal showed better results in relation to its structural counterpart, managing to have an antinociceptive effect in the formalin and hot plate tests with a lower concentration (100 mg/kg) and presenting fewer side effects, however, the this study was not able to elucidate the mechanism of action of this isomer despite having activity in studies with substances that act on specific targets such as glutamate and capsaicin, its activity was not reversed with the use of antagonists for pathways related to nociception. While the (R)-(+) citronellal isomer, despite showing total activity only at a concentration of 150 mg/kg, was able to determine its mechanism of action related to the opioid pathway by reversing its activity by the antagonist naloxone, being this is a pathway already correlated with nociception control treatments, however, it is also related to some unwanted side effects. In this way, new studies are sought to elucidate the mechanism related to the isomer (S)-(-) citronellal and a possibility of use in other areas related to the treatment of pain or inflammation.

Devido à natureza complexa da dor e a sua participação de aspectos físicos, cognitivos, psicológicos e comportamentais, o manejo da dor possui diversas abordagens. O uso de plantas medicinais em países em desenvolvimento é bastante expressivo. Buscando novas opções para o tratamento de denças emergentes ou debilitantes. Portanto, o presente estudo busca elucidar os efeitos do monoterpeno, citronelal, diferenciando sua atividade pelos isômeros (R)-(+) e (S)-(-) citronelal. O estudo utilizou diversos métodos para avaliar os efeitos dos isômeros de citronelal na coordenação motora, resposta nociceptiva e o envolvimento de vias opioides, glutamatérgicas e de receptores transitórios. Os métodos incluíram testes de rota-rod, placa quente e formalina, além do uso de inibidores e agonistas específicos. Os dados foram analisados estatisticamente com um intervalo de confiança de 95%. Ambos os isomeros não afetaram significativamente a coordenação motora dos animais em estudo. O isômero (S)-(-) citronelal apresentou melhores resultados em relação ao seu homólogo estrutural, conseguindo ter um efeito antinociceptivo nos testes de formalina e placa quente com menor concentração (100 mg/kg) e apresentando menos efeitos colaterais, entretanto, o presente estudo não foi capaz de elucidar o mecanismo de ação deste isomero apesar de ter atividadade em estudos com substancias que agem em alvos específicos como glutamato e capsaicina, sua atividade não foi revertida com a utilização de antagonistas para as vias relacionadas à nocicepção. Enquanto o isômero (R)-(+) citronelal, apesar de apresentar de apresentar total atividade somente na concentração de 150 mg/kg, foi capaz de determinar seu mecanismo de ação relacionado à via opióide pela reversão da sua atividade pelo antagonista naloxona, sendo esta uma via já correlacionada com os tratamentos de controle da a nocicepção, no entanto, também está relacionada a alguns efeitos colaterais indesejados. Desta forma busca-se novos estudos para elucidação do mecanismo relacionados ao isomero (S)-(-) citronellal e uma possibilidade de utilização em outras areas relacionadas ao tratamento da dor ou inflamação.

Impact of yeast on the characteristics of Sauvignon Blanc wines from the Campanha Gaúcha Region / Impacto de leveduras nas características de vinhos Sauvignon Blanc da Região da Campanha Gaúcha

The objective of this work was to evaluate the effect of different commercial strains of Saccharomyces cerevisiae on the characteristics of Sauvignon Blanc wines produced in the Campanha Gaúcha region. The must obtained by mechanical destemming and crushing was treated with 150 mg L of potassium metabisulfite and pectolitic enzymes and subject to enzymatic hidrolisis and débourbage for 24h00 at 4°C. The must was divided into glass fermenters of 4,6 L, in triplicate for each of the four treatments, which were defined by the inoculation of 25g hL-1 of dry yeast: T1 - Zymaflore X5® (Laffort, Bordeaux, France); T2 - AWRI 796 (AB Biotek, Sydney, Australia); T3 - Maurivim PDM (AB Biotek, Sydney, Australia); T4 - 50% Zymaflore X5® + 50% AWRI 796. The wines were analyzed, regarding: ethanol; reducing sugars; total acidity; volatile acidity; pH; dry matter; and ash. The most significant differences were observed in: ethanol; reducing sugars; pH; dry matter. Among the treatments inoculated with single strains, T1 showed higher ethanol and lower reducing sugars content. T3 presented results similar to T1. T2 resulted in higher content of reducing sugars and consequently lower ethanol levels. The mixed fermentation (T4) achieved the lowest levels of reducing sugars among the treatments. It suggests that mixed fermentations could be an alternative to produce drier wines.(AU)

O objetivo do trabalho foi avaliar o efeito de leveduras Saccharomyces cerevisiae comerciais nas características de vinhos Sauvignon Blanc, da região da Campanha Gaúcha, Brasil. Após desengace e esmagamento das uvas, o mosto foi sulfitado (150 mg L de metabissulfito de potássio), submetido a 24h00 de débourbage. Após, o mosto foi dividido em garrafões de vidro de 4,6 L, para realização da fermentação alcoólica, em triplicata. Os tratamentos constituíram-se pela inoculação de 25 g hL-1leveduras desidratadas: T1 - Zymaflore X5® (Laffort, Bordeaux, França); T2 - AWRI 796® (AB Biotek, Sydney, Austrália); T3 - Maurivin PDM® (AB Biotek, Sydney, Austrália), T4 - 50% Zymaflore X5® + 50% de AWRI 796®. As variáveis analisadas nos vinhos foram: etanol; açúcares redutores; acidez total; acidez volátil; pH; extrato seco; cinzas. As diferenças mais significativas foram observadas para: etanol; açúcares redutores; pH; extrato seco. Dentre os tratamentos com inoculação de cepas únicas, T1 apresentou maior graduação alcoólica e baixo teor de açúcares redutores. T3 apresentou resultados semelhantes a T1. T2 apresentou menor graduação alcoólica e maior teor de açúcares redutores. T4 apresentou os níveis mais baixos de açúcares redutores dentre os tratamentos. Isso sugere que a inoculação de mais de uma cepa pode ser uma alternativa para produzir vinhos mais secos.(AU)

O uso de opioides em ruminantes / Use of opioids in ruminants

Os ruminantes, como todos os animais, têm capacidade de sentir dor, a qual pode ser causada por doenças ou procedimentos frequentemente praticados, como a descorna e a orquiectomia. Todavia, nesses animais, o uso de analgésicos ainda é menos comum do que o ideal, sendo necessária uma maior conscientização, além de estudos com fármacos e protocolos, capazes de aliviar a dor de maneira eficaz nessas espécies. Neste contexto, destacam-se os opioides, potentes fármacos analgésicos, sendo ótima opção para o controle da dor; entretanto, seu uso ainda é pouco frequente em ruminantes, os estudos são limitados e encontram resultados variáveis. Diante disso, o objetivo deste trabalho foi revisar os benefícios do controle da dor em ruminantes, através do uso de opioides, elucidando as possibilidades analgésicas com diferentes fármacos desta classe e vias de administração, a fim de incentivar o uso pelos médicos veterinários de ruminantes, com base na literatura já publicada sobre este assunto; uma vez que, mesmo com alguns resultados variáveis, diversos estudos têm demonstrado o potencial antinociceptivo dos opioides em ruminantes, além dos efeitos sedativos, destacando o potencial analgésico principalmente por vias de administração alternativas, como o fentanil transdérmico e a via epidural para diversos opioides, os quais tornam-se opções importantes a serem consideradas nos protocolos analgésicos para ruminantes.

Ruminants, as all other animals, feel pain, which can be caused by diseases or frequently practiced procedures, such as dehorning and orchiectomy. However, in these animals the use of analgesics is still less common than ideal, requiring greater awareness, in addition to studies with drugs and protocols capable of effectively relieving pain in these species. In this context, opioids, potent analgesic drugs, are highlighted as a great option for pain control. Nevertheless, their use is still infrequent in ruminants, where studies are limited and show varied results. Therefore, the objective of this study was to review the benefits of pain control in ruminants through opioids, elucidating the analgesic possibilities with different drugs of this class and administration routes to encourage the use by veterinarians of ruminants, based on the literature already published on this subject. Even with some variable results, several studies have demonstrated the antinociceptive potential of opioids in ruminants, in addition to the sedative effects, highlighting the analgesic potential mainly through alternative routes of administration, such as transdermal fentanyl and the epidural route for various opioids, which become important options to be considered in analgesic protocols for ruminants.

Psychometric properties of the Brazilian version of the Canine Brief Pain Inventory (CBPI) for dogs with clinical signs of osteoarthritis and preliminary evidence of its clinical utility / Propriedades psicométricas da versão brasileira do Breve Inventário de Dor Canina (BIDC) em cães com sinais clínicos de osteoartrite e evidências preliminares da sua utilização clínica

This study aimed to determine the psychometric properties and clinical use of the Brazilian version of the Canine Brief Pain Inventory (CBPI) in dogs with hip dysplasia (HD). Forty-three dogs with HD and 16 clinically normal dogs were enrolled. The HD dogs were treated daily with 4.4mg/kg carprofen (GT = 21) or placebo (GP = 19), for four weeks. Owners completed the CBPI at two weeks (W-2) and immediately before the start of the treatment (W0), two (W2) and four (W4) weeks during treatment, and two weeks (W6) after the end of treatment. The internal structure was accessed, and the Cronbach's alpha coefficient was 0.97, indicating the high internal consistency of the instrument. Principal Component Analysis (PCA) suggested the retention of one component, which accounted for 78% of the variability. The ROC curve analysis concluded that the score 3 has an excellent performance to discriminate between normal and possible HD dogs (AUC of 0.973). There was no difference between dogs treated with carprofen versus placebo. The instrument in Portuguese showed construct and criterion validity and reliability to be used in dogs with HD.

Objetivou-se determinar as propriedades psicométricas e a utilidade clínica do Breve Inventário de Dor Canina na língua portuguesa, em cães com displasia coxofemoral (DCF). O inventário foi preenchido por tutores de 43 animais com DCF e por 16 tutores de cães saudáveis. Os animais com DCF foram tratados com carprofeno 4,4mg/kg (GT = 21) ou placebo (GP =19), administrados uma vez ao dia durante quatro semanas. As avaliações foram realizadas duas semanas e imediatamente antes do tratamento, duas e quatro semanas durante o tratamento e após duas semanas do término do tratamento. A estrutura interna calculada pelo alfa de Cronbach = 0,97 indicou alta consistência dos dados. A análise dos componentes principais identificou a retenção de apenas um componente responsável por 78% da variabilidade dos dados. A análise da curva ROC indicou que o escore 3 discrimina cães saudáveis de cães com possível DCF (ASC de 0,973). Não houve diferença entre os cães tratados com carprofeno daqueles que receberam placebo. O questionário apresentou validade de constructo e critério e confiabilidade e pode-se empregá-lo para avaliar a dor crônica em cães com osteoartrite em países de língua portuguesa.

Tratamento de reação inflamatória cerebral após Acidente Vascular Encefálico (AVE) e Trauma Cranioencefálico (TCE) em cão / Treatment of inflammatory brain reaction after Brain Vascular Accident (CVA) and Traumatic Brain Injury (TBI) in a dog / Tratamiento de la reacción inflamatoria cerebral después de un Accidente Vascular Cerebral (ACV) y Traumatismo Cerebral (TEC) en un perro

O presente relato de caso tem por objetivo descrever a ocorrência de quadro neurológico tardio, pós-trauma cranioencefálico em um paciente canino, atendido em clínica veterinária particular. O animal foi avaliado clinicamente, com alterações neurológicas intensas de ataxia vestibular, andar compulsivo, paresia, perda de propriocepção nos quatro membros, miose bilateral, anisocoria, entre outros. O diagnóstico foi presuntivo, auxiliado por ressonância magnética, e baseado no histórico detalhado. O tratamento clínico foi determinado empiricamente, a base de dexametasona comercial, associada a metionina, nicotinamida e piridoxina. O paciente apresentou melhora clínica rápida, diminuindo inflamação encefálica e desaparecimento de quadro neurológico.

The present case report aims to describe the occurrence of a late neurological condition, after traumatic brain injury in a canine patient, treated at a private veterinary clinic. The animal was clinically evaluated, with severe neurological alterations of vestibular ataxia, compulsive gait, paresis, loss of proprioception in all four limbs, bilateral miosis, anisocoria, among others. Diagnosis was presumptive, aided by MRI, and based on detailed history. Clinical treatment was empirically determined, based on commercial dexamethasone, associated with methionine, nicotinamide and pyridoxine. The patient showed rapid clinical improvement, with no neurological picture.

El presente reporte de caso tiene como objetivo describir la aparición de una condición neurológica tardía, posterior a un traumatismo craneoencefálico en un paciente canino, atendido en una clínica veterinaria privada. El animal fue evaluado clínicamente, con alteraciones neurológicas severas de ataxia vestibular, marcha compulsiva, paresia, pérdida de propiocepción en las cuatro extremidades, miosis bilateral, anisocoria, entre otras. El diagnóstico fue presuntivo, ayudado por resonancia magnética y basado en una historia clínica detallada. El tratamiento clínico se determinó empíricamente, a base de dexametasona comercial, asociada a metionina, nicotinamida y piridoxina. El paciente presentó una rápida mejoría clínica sin cuadro neurológico.

Pinching or stinging? Comparing prey capture among scorpions with contrasting morphologies

Background: Scorpions can use their pincers and/or stingers to subdue and immobilize their prey. A scorpion can thus choose between strategies involving force or venom, or both, depending on what is required to subdue its prey. Scorpions vary greatly in the size and strength of their pincers, and in the efficacy of their venom. Whether this variability is driven by their defensive or prey incapacitation functionis unknown. In this study, we test if scorpion species with different pincer morphologies and venom efficacies use these weapons differently during prey subjugation. To that end, we observed Opisthacanthus elatus and Chactas sp. with large pincers and Centruroides edwardsii and Tityus sp. with slender pincers. Methods: The scorpion pinch force was measured, and behavioral experiments were performed with hard and soft prey (Blaptica dubia and Acheta domesticus). Stinger use, sting frequency and immobilization time were measured. Results: We found that scorpions with large pincers such as O. elatus produce more force and use the stinger less, mostly subjugating prey by crushing them with the pincers. In C. edwardsii and Tityus sp. we found they use their slender and relatively weak pincers for holding the prey, but seem to predominantly use the stinger to subjugate them. On the other hand, Chactas sp. uses both strategies although it has a high pinch force. Conclusions: Our results show that scorpionspecies with massive pincers and high pinch force as O. elatus use the stinger less for prey subjugation than scorpionspecies with slenderpincers.(AU)

Analgesic effects of Phα1ß toxin: a review of mechanisms of action involving pain pathways

Phα1ß is a neurotoxin purified from spider venom that acts as a high-voltage-activated (HVA) calcium channel blocker. This spider peptide has shown a high selectivity for N-type HVA calcium channels (NVACC) and an analgesic effect in several animal models of pain. Its activity was associated with a reduction in calcium transients, glutamate release, and reactive oxygen species production from the spinal cord tissue and dorsal ganglia root (DRG) in rats and mice. It has been reported that intrathecal (i.t.) administration of Phα1ß to treat chronic pain reverted opioid tolerance with a safer profile than ω-conotoxin MVIIA, a highly selective NVACC blocker. Following a recent development of recombinant Phα1ß (CTK 01512-2), a new molecular target, TRPA1, the structural arrangement of disulphide bridges, and an effect on glial plasticity have been identified. CTK 01512-2 reproduced the antinociceptive effects of the native toxin not only after the intrathecal but also after the intravenous administration. Herein, we review the Phα1ß antinociceptive activity in the most relevant pain models and its mechanisms of action, highlighting the impact of CTK 01512-2 synthesis and its potential for multimodal analgesia.

Pain-related toxins in scorpion and spider venoms: a face to face with ion channels

Pain is a common symptom induced during envenomation by spiders and scorpions. Toxins isolated from their venom have become essential tools for studying the functioning and physiopathological role of ion channels, as they modulate their activity. In particular, toxins that induce pain relief effects can serve as a molecular basis for the development of future analgesics in humans. This review provides a summary of the different scorpion and spider toxins that directly interact with pain-related ion channels, with inhibitory or stimulatory effects. Some of these toxins were shown to affect pain modalities in different animal models providing information on the role played by these channels in the pain process. The close interaction of certain gating-modifier toxins with membrane phospholipids close to ion channels is examined along with molecular approaches to improve selectivity, affinity or bioavailability in vivo for therapeutic purposes.(AU)

Analgesic effects of Phalfa1 beta toxin: a review of mechanisms of action involving pain pathways

Phα1ß is a neurotoxin purified from spider venom that acts as a high-voltage-activated (HVA) calcium channel blocker. This spider peptide has shown a high selectivity for N-type HVA calcium channels (NVACC) and an analgesic effect in several animal models of pain. Its activity was associated with a reduction in calcium transients, glutamate release, and reactive oxygen species production from the spinal cord tissue and dorsal ganglia root (DRG) in rats and mice. It has been reported that intrathecal (i.t.) administration of Phα1ß to treat chronic pain reverted opioid tolerance with a safer profile than ω-conotoxin MVIIA, a highly selective NVACC blocker. Following a recent development of recombinant Phα1ß (CTK 01512-2), a new molecular target, TRPA1, the structural arrangement of disulphide bridges, and an effect on glial plasticity have been identified. CTK 01512-2 reproduced the antinociceptive effects of the native toxin not only after the intrathecal but also after the intravenous administration. Herein, we review the Phα1ß antinociceptive activity in the most relevant pain models and its mechanisms of action, highlighting the impact of CTK 01512-2 synthesis and its potential for multimodal analgesia.

Avaliação da eficácia do sistema rigeneracon no tratamento de lesões de calvária em ratos / [Evaluation of the efficacy of the rigeneracon system in the treatment of calvary injuries in rats]

Defeitos ósseos constituem um problema de saúde global. O sistema Rigenera permite a extração de microenxertos ricos em células-tronco mesenquimais (CTMs). Objetivou-se avaliar o processo de regeneração óssea por enxertos obtidos pelo sistema Rigenera em defeitos críticos na calvária de ratos. Foram utilizados 18 ratos Wistar, machos, pesando 285±29g, distribuídos em três grupos (n=6), sendo cada animal controle de si mesmo, denominados G15-Controle e G15-Tratado (15 dias); G30-Controle e G30-Tratado (30 dias) e G60-Controle e G60-Tratado (60 dias). Foram realizadas duas lesões de 5mm de diâmetro em cada antímero da calvária. Nos grupos tratados, foram utilizados microenxertos autólogos de cartilagem xifoide, obtidos pelo sistema Rigenera. O defeito contralateral serviu como controle em todos os animais. Os animais foram eutanasiados aos 15, 30 e 60 dias após a cirurgia, e as amostras foram processadas para a histoquímica. Nos grupos controle, não foram observados sinais de regeneração óssea, enquanto nos grupos tratamento foram verificadas áreas de formação óssea e tecido mesenquimal ativado. O sistema Rigenera foi eficiente na obtenção de microenxertos autólogos, para terapia celular em defeito crítico de calvária de ratos. Com o aprimoramento do protocolo, o sistema Rigenera poderá ser amplamente utilizado no tratamento de lesões ósseas.(AU)

Bone defects are a global health problem. The Rigenera system allows the extraction of micro grafts rich in mesenchymal stem cells (MSCs). The objective of this study was to evaluate the bone regeneration process by grafts obtained by the Rigenera system in defects in the rats calvarian. Eighteen male Wistar rats were used, weighing 285 ± 29g, distributed in three groups (n = 6), where each animal was treatment and control, called G15-Control and G15-Treated (15 days); G30-Control and G30-Treated (30 days) and G60-Control and G60-Treated (60 days). Two 5mm diameter lesions were performed on each calvaria side. In the treated groups, autologous micrograft from xiphoid cartilage, obtained by the Rigenera system, were used. The other defect served as a control in all animals. The animals were euthanized at 15, 30 and 60 days after the surgery and the samples were processed for histochemistry. In the control groups, no signs of bone regeneration were observed, while in the treatment groups, areas of bone formation and activated mesenchymal tissue were verified. The Rigenera system was efficient in obtaining autologous micrograft for cell therapy in a critical calvaria defect in rats. Rigenera system can be widely used in the treatment of bone injuries.(AU)

O uso da acupuntura no trauma crânio encefálico relato de caso

O trauma cranioencefálico (TCE) é uma emergência com altos índices de morte, pode acarretar sequelas motoras e o prognóstico depende da rapidez no tratamento. A Medicina Tradicional Chinesa é usada no tratamento de animais há milhares de anos e a acupuntura atua no sistema nervoso estimulando o mecanismo de compensação e equilíbrio agindo sobre a dor, liberando opioides endógenos que auxiliam na recuperação de sequelas e melhoram a expectativa de vida. Foi atendido um felino, vítima de TCE causado por atropelamento de moto. Realizou-se o tratamento com acupuntura apresentando melhora considerável do quadro.(AU)

Traumatic brain injury (TBI) is an emergency with high death rates, can lead to motor sequelae and prognosis depends on the speed of treatment. Traditional Chinese Medicine has been used in the treatment of animals for thousands of years and acupuncture acts on the nervous system by stimulating the mechanism of compensation and balance by acting on pain, releasing endogenous opioids that aid in the recovery of sequelae and improve life expectancy. A feline victim of TBI was treated for a motorcycle hit-and-run. Acupuncture treatment was performed, with considerable improvement of the condition.(AU)

O uso da acupuntura no trauma crânio encefálico relato de caso

O trauma cranioencefálico (TCE) é uma emergência com altos índices de morte, pode acarretar sequelas motoras e o prognóstico depende da rapidez no tratamento. A Medicina Tradicional Chinesa é usada no tratamento de animais há milhares de anos e a acupuntura atua no sistema nervoso estimulando o mecanismo de compensação e equilíbrio agindo sobre a dor, liberando opioides endógenos que auxiliam na recuperação de sequelas e melhoram a expectativa de vida. Foi atendido um felino, vítima de TCE causado por atropelamento de moto. Realizou-se o tratamento com acupuntura apresentando melhora considerável do quadro.

Traumatic brain injury (TBI) is an emergency with high death rates, can lead to motor sequelae and prognosis depends on the speed of treatment. Traditional Chinese Medicine has been used in the treatment of animals for thousands of years and acupuncture acts on the nervous system by stimulating the mechanism of compensation and balance by acting on pain, releasing endogenous opioids that aid in the recovery of sequelae and improve life expectancy. A feline victim of TBI was treated for a motorcycle hit-and-run. Acupuncture treatment was performed, with considerable improvement of the condition.

Silage quality from intercropping corn and soybean managed with inoculant Azospirillum brasilense and nitrogen fertilization

ABSTRACT Intercropping grass and soybeans can promote beneficial effects on the productivity of the explored area due to the better soil nitrogen utilization and protein concentrate savings through the use of mixed silage. This study aimed to evaluate the bromatological quality, digestibility, and fermentation profile of silage produced by intercropping corn and soybean. Eight treatments were tested by combining inoculation with Azospirillum brasilense and nitrogen fertilization applied to corn intercropped in rows alternated with soybean. Corn and soybean monocultures were also tested. The morphological compositions of soybean and corn plants in the mass of the ensiled material were determined. After crushing, the material was ensiled in PVC tubes for 60 days, and the silage quality was determined after fermentation. Treatments did not affect the morphological composition of soybean and corn plants in the ensiled material from intercropping. The proportions of soybean leaves, stems, and pods represented less than 5% of the total ensiled mass of intercropping. On the other hand, the morphological composition of corn plants was high, similar to that of monoculture. Thus, the silage from intercropping showed similar quality standards to that of corn monoculture, and the bromatological composition, digestibility, pH, energy content, and profile of organic acids were suitable for good quality silage.

RESUMO A consorciação entre gramínea e soja pode promover efeitos benéficos sobre a produtividade da área explorada, devido ao melhor aproveitamento do nitrogênio no solo e economia de concentrados proteicos pelo uso de silagem mista. Objetivou-se neste trabalho avaliar a qualidade bromatológica, digestibilidade e o perfil de fermentação da silagem produzida pelo consórcio entre milho e soja. Foram testados oito tratamentos, combinando a inoculação com Azospirillum brasilense e a adubação nitrogenada aplicada no milho consorciado em fileiras alternadas com a soja. Os monocultivos de milho e de soja também foram testados. Foram determinadas as composições morfológicas das plantas de soja e milho na massa da matéria ensilada. Após triturado, o material foi ensilado em tubos de PVC por 60 dias e após a fermentação foi determinada a qualidade da silagem. Os tratamentos não afetaram a composição morfológica das plantas de soja e de milho no material ensilado oriundo do consórcio. As proporções de folhas, hastes e vagens de soja representaram menos de 5% do total da massa ensilada do consórcio. Por outro lado, a composição morfológica das plantas de milho foi elevada semelhante ao do monocultivo. Assim, a silagem proveniente do consórcio apresentou padrões semelhantes de qualidade ao do milho exclusivo, sendo que a composição bromatológica, digestibilidade, pH, teores de energia e perfil de ácidos orgânicos foram adequados para uma silagem de boa qualidade.

Silage quality from intercropping corn and soybean managed with inoculant Azospirillum brasilense and nitrogen fertilization / Qualidade da silagem do consórcio entre milho e soja manejado com inoculante Azospirillum brasilense e adubação nitrogenada

Intercropping grass and soybeans can promote beneficial effects on the productivity of the explored area due to the better soil nitrogen utilization and protein concentrate savings through the use of mixed silage. This study aimed to evaluate the bromatological quality, digestibility, and fermentation profile of silage produced by intercropping corn and soybean. Eight treatments were tested by combining inoculation with Azospirillum brasilense and nitrogen fertilization applied to corn intercropped in rows alternated with soybean. Corn and soybean monocultures were also tested. The morphological compositions of soybean and corn plants in the mass of the ensiled material were determined. After crushing, the material was ensiled in PVC tubes for 60 days, and the silage quality was determined after fermentation. Treatments did not affect the morphological composition of soybean and corn plants in the ensiled material from intercropping. The proportions of soybean leaves, stems, and pods represented less than 5% of the total ensiled mass of intercropping. On the other hand, the morphological composition of corn plants was high, similar to that of monoculture. Thus, the silage from intercropping showed similar quality standards to that of corn monoculture, and the bromatological composition, digestibility, pH, energy content, and profile of organic acids were suitable for good quality silage.

A consorciação entre gramínea e soja pode promover efeitos benéficos sobre a produtividade da área explorada, devido ao melhor aproveitamento do nitrogênio no solo e economia de concentrados proteicos pelo uso de silagem mista. Objetivou-se neste trabalho avaliar a qualidade bromatológica, digestibilidade e o perfil de fermentação da silagem produzida pelo consórcio entre milho e soja. Foram testados oito tratamentos, combinando a inoculação com Azospirillum brasilense e a adubação nitrogenada aplicada no milho consorciado em fileiras alternadas com a soja. Os monocultivos de milho e de soja também foram testados. Foram determinadas as composições morfológicas das plantas de soja e milho na massa da matéria ensilada. Após triturado, o material foi ensilado em tubos de PVC por 60 dias e após a fermentação foi determinada a qualidade da silagem. Os tratamentos não afetaram a composição morfológica das plantas de soja e de milho no material ensilado oriundo do consórcio. As proporções de folhas, hastes e vagens de soja representaram menos de 5% do total da massa ensilada do consórcio. Por outro lado, a composição morfológica das plantas de milho foi elevada semelhante ao do monocultivo. Assim, a silagem proveniente do consórcio apresentou padrões semelhantes de qualidade ao do milho exclusivo, sendo que a composição bromatológica, digestibilidade, pH, teores de energia e perfil de ácidos orgânicos foram adequados para uma silagem de boa qualidade.

Silage quality from intercropping corn and soybean managed with inoculant Azospirillum brasilense and nitrogen fertilization / Qualidade da silagem do consórcio entre milho e soja manejado com inoculante Azospirillum brasilense e adubação nitrogenada

Intercropping grass and soybeans can promote beneficial effects on the productivity of the explored area due to the better soil nitrogen utilization and protein concentrate savings through the use of mixed silage. This study aimed to evaluate the bromatological quality, digestibility, and fermentation profile of silage produced by intercropping corn and soybean. Eight treatments were tested by combining inoculation with Azospirillum brasilense and nitrogen fertilization applied to corn intercropped in rows alternated with soybean. Corn and soybean monocultures were also tested. The morphological compositions of soybean and corn plants in the mass of the ensiled material were determined. After crushing, the material was ensiled in PVC tubes for 60 days, and the silage quality was determined after fermentation. Treatments did not affect the morphological composition of soybean and corn plants in the ensiled material from intercropping. The proportions of soybean leaves, stems, and pods represented less than 5% of the total ensiled mass of intercropping. On the other hand, the morphological composition of corn plants was high, similar to that of monoculture. Thus, the silage from intercropping showed similar quality standards to that of corn monoculture, and the bromatological composition, digestibility, pH, energy content, and profile of organic acids were suitable for good quality silage.(AU)

A consorciação entre gramínea e soja pode promover efeitos benéficos sobre a produtividade da área explorada, devido ao melhor aproveitamento do nitrogênio no solo e economia de concentrados proteicos pelo uso de silagem mista. Objetivou-se neste trabalho avaliar a qualidade bromatológica, digestibilidade e o perfil de fermentação da silagem produzida pelo consórcio entre milho e soja. Foram testados oito tratamentos, combinando a inoculação com Azospirillum brasilense e a adubação nitrogenada aplicada no milho consorciado em fileiras alternadas com a soja. Os monocultivos de milho e de soja também foram testados. Foram determinadas as composições morfológicas das plantas de soja e milho na massa da matéria ensilada. Após triturado, o material foi ensilado em tubos de PVC por 60 dias e após a fermentação foi determinada a qualidade da silagem. Os tratamentos não afetaram a composição morfológica das plantas de soja e de milho no material ensilado oriundo do consórcio. As proporções de folhas, hastes e vagens de soja representaram menos de 5% do total da massa ensilada do consórcio. Por outro lado, a composição morfológica das plantas de milho foi elevada semelhante ao do monocultivo. Assim, a silagem proveniente do consórcio apresentou padrões semelhantes de qualidade ao do milho exclusivo, sendo que a composição bromatológica, digestibilidade, pH, teores de energia e perfil de ácidos orgânicos foram adequados para uma silagem de boa qualidade.(AU)

Frequency and intensity of pain symptoms detected during classic massage sessions of selected body parts in purebred Arabian racing horses

We analysed the frequency of symptoms and degree of muscle pain in selected body parts of racing horses assessed during classic massage sessions. The influence of horse's sex on obtained results was considered. The potential for the early determination of pain in horses by analysing their behaviour and cardiac parameters during a massage session was also evaluated. The study was conducted on 20 three-year-old purebred Arabian horses during one racing season. In the racing season, cyclic classic massage sessions were performed, during which the frequency of symptoms and the degree of pain in the neck, back, croup, front limbs, and hind limbs were analysed. A behavioural assessment of the horses was conducted, and cardiac parameters were analysed. During massage, the frequency of pain symptoms in front limbs amounted to 26, while in croup, it did not exceed 6. The studied horses were most susceptible to pain in the front limbs and in the back, with greater severity in stallions than in mares. An assessment of the frequency and severity of pain symptoms should not be based on changes in behaviour of horses or on cardiac parameters (HR and LF:HF ratio) during massage sessions. However, these methods can be applied after pain reactions intensify. Meanwhile, qualified masseurs can diagnose slight muscle pain during massage sessions.(AU)

Neurological modulation of facial expressions in pigs and implications for production

The facial expressions of animals constitute a means of manifesting emotions, fulfilling functions related to social interactions in several species. Interest has come to use facialexpressions as a tool for predicting intentions; in the case of aggression, for example, impeding attacks between individuals prevents negative economic and emotional impacts. Recent research has sought to associate facial expressions with painful events as tail-cropping and castration on the assumption thatobjective evaluations of the presence or absence of grimaces in pigs' snouts may allow us to determine the intensity of the pain they perceive. Furthermore, to continue developing scales that will complement the existing piglet grimace scale (PGS), which is not yet fully-validated in terms of reliability. Another area of research is interpreting expressions of fear during traumatic events like pre-slaughter handling. Also, the phenomenon of emotional contagion in groups of animals suggests the need to focus on their emotions, since recognizing them could allow us to prevent alterations of meat quality.These approaches reveal the need for a detailed compilation of the facial expressions of pigs based on current scientific literature regarding emotions, alimentation, and pain-related behavioral responses in pigs.(AU)

Neurological modulation of facial expressions in pigs and implications for production

The facial expressions of animals constitute a means of manifesting emotions, fulfilling functions related to social interactions in several species. Interest has come to use facialexpressions as a tool for predicting intentions; in the case of aggression, for example, impeding attacks between individuals prevents negative economic and emotional impacts. Recent research has sought to associate facial expressions with painful events as tail-cropping and castration on the assumption thatobjective evaluations of the presence or absence of grimaces in pigs' snouts may allow us to determine the intensity of the pain they perceive. Furthermore, to continue developing scales that will complement the existing piglet grimace scale (PGS), which is not yet fully-validated in terms of reliability. Another area of research is interpreting expressions of fear during traumatic events like pre-slaughter handling. Also, the phenomenon of emotional contagion in groups of animals suggests the need to focus on their emotions, since recognizing them could allow us to prevent alterations of meat quality.These approaches reveal the need for a detailed compilation of the facial expressions of pigs based on current scientific literature regarding emotions, alimentation, and pain-related behavioral responses in pigs.

Fractions of Selaginella convoluta (Arn) Spring (Selaginellaceae) attenuate the nociceptive behavior events in mice / Frações de Selaginella convoluta (Ar) Spring (Selaginellaceae) atenuam a resposta nociceptiva em camundongos

Selaginella convoluta (Arn.) Spring is a species popularly known as "jericó", and used in folk medicine as analgesic and anti-inflammatory. This study aimed to investigate in mice the antinociceptive and anti-inflammatory activities of the hexane (Sc-Hex) and chloroform (Sc-CHCl3) fractions (100, 200 and 400 mg/kg) obtained by partition of crude ethanol extract from S. convoluta. The preliminary phytochemical analysis of the fractions was performed. Antinociceptive activity was evaluated by writhing, formalin and hot-plate tests. Anti-inflammatory activity was evaluated using carrageenan-induced pleurisy. The rota-rod test was used to evaluate motor coordination. Preliminary phytochemical screening showed that the Sc-Hex and the Sc-CHCl3 were positive for the presence of flavonoids, anthracene derivatives, quinones, triterpenes and steroids. Inhibition of writhing was observed for fractions tested. The Sc-Hex at all doses tested was effective in reducing the nociceptive behavior produced by formalin only in the second phase. However, the Sc-CHCl3 decreased the paw licking time in the first and second phases. In the hot plate no significant effect was observed for any fraction. In the rota-rod test, treated mice did not demonstrate any significant motor performance changes. In the carrageenan-induced pleurisy, Sc-CHCl3 (200 mg/kg) reduced cell migration to the pleural cavity. These results reveal the antinociceptive properties of S. convoluta , which support, in part, its traditional use, since the fractions did not presented significant activity in the inflammatory response profile. We further verify that this antinociceptive effect could be by activation of nociceptive peripheral pathway.(AU)

Selaginella convoluta é uma espécie popularmente conhecida como "jericó", e usada na medicina popular como analgésica e anti-inflamatória. Este estudo teve como objetivo investigar em camundongos as atividades antinociceptiva e anti-inflamatória das frações hexânica (Sc-Hex) e clorofórmica (Sc-CHCl3) (100, 200 e 400 mg/kg) obtidas por partição do extrato etanólico bruto de S. convoluta . A análise fitoquímica preliminar das frações foi realizada. A atividade antinociceptiva foi avaliada pelos testes de contorções abdominais, formalina e placa quente. A atividade anti-inflamatória foi avaliada usando pleurisia induzida por carragenina. O teste do rota-rod foi utilizado para avaliar a coordenação motora. A triagem fitoquímica preliminar mostrou que Sc-Hex e Sc-CHCl3 apresentaram reação positiva para a presença de flavonoides, derivados antracênicos, quinonas, triterpenos e esteroides. A inibição das contorções foi observada para as frações testadas. Sc-Hex em todas as doses testadas foi efetivo na redução do comportamento nociceptivo produzido pela formalina somente na segunda fase. No entanto, Sc-CHCl3 diminuiu o tempo de lambida da pata na primeira e segunda fases. Na placa quente, nenhum efeito significativo foi observado para qualquer fração. No teste do rota-rod os camundongos tratados não demonstraram mudanças significativas na coordenação motora. Na pleurisia induzida por carragenina, Sc-CHCl3 (200 mg/kg) reduziu a migração celular para a cavidade pleural. Estes resultados revelam a propriedade antinociceptiva de S. convoluta, justificando, em parte, seu uso tradicional, uma vez que os extratos não apresentaram atividade significativa no perfil de resposta inflamatória. Verificamos ainda que esse efeito antinociceptivo mostrou-se ligado à ativação da via periférica nociceptiva.(AU)

Acute-on-chronic subdural cranial hematoma in a dog: case report / Hematoma craniano subdural crônico-agudizado em um cão: relato de caso

A ten-month-old male Yorkshire terrier dog was evaluated via CT four months after traumatic brain injury. The head CT scan showed dilated ventricles associated with a peripheral crescent-shaped collection of blood near the right parietal bone with a mineralized area. The visceral layer of the hematoma was hyperattenuating on the native scan and showed moderate contrast enhancement after administration of intravenous iodinated contrast medium. No fractures were identified in the calvarium. These findings were compatible with acute-on-chronic calcified subdural hematoma, which have been described with more detail in humans. This is the first report to include the description of the imaging findings related to this condition using computed tomography in a dog.(AU)

Um cão Yorkshire Terrier, de 10 meses de idade, foi avaliado por tomografia computadorizada, quatro meses após lesão encefálica de origem traumática. A tomografia da cabeça demonstrou dilatação dos ventrículos associada com coleção de sangue periférico, em formato crescente, próxima ao osso parietal direito, com uma área mineralizada interna. A camada visceral do hematoma era hiperatenuante no exame pré-contraste e demonstrou moderado aumento de atenuação radiográfica após a administração de contraste iodado intravenoso. Fraturas no calvário não foram identificadas. Esses achados foram compatíveis com agudização de hematoma subdural crônico calcificado, condição que tem sido descrita com mais detalhes em humanos. Este é o primeiro relato que descreve os achados de imagem, por tomografia computadorizada, em um cão, relacionados a essa condição.(AU)