Bacillus spp. -fermented mixture in goat starter diets on growth performance, blood, and carcass and gastrointestinal traits

The aim of this study was to evaluate the effects of incorporating Bacillus spp.-fermented mixture (FM; 55% soybean meal and 45% feather meal) in the starter feed of goat kids, specifically its effects on growth performance, blood profile, carcass characteristics, and gastrointestinal traits; the FM protein profile was also evaluated. Seventy-five four-week-old male dairy goat kids were randomly assigned to three different starter diet groups containing B, D, or J strains of 5% Bacillus spp. FM (BG, DG, and JG), a commercial starter diet group (CG), and a starter diet group without FM (NG). During the starter phase (0-6 weeks), the average daily gain was higher in JG and BG goats than in the NG, with the average daily gain and feed conversion in BG found to be best at 6-8 weeks. Furthermore, the intestinal villi and papillae height in the ventral and dorsal sac of BG goats were higher than those in the other groups. The SDS-PAGE analysis of the FM samples further indicated that low molecular weight peptides in FM significantly increased after fermentation. Thus, using a suitable Bacillus sp. to ferment soybean and feather meals can increase the available nutrients. Taken together, these results indicate that adding 5% Bacillus spp.-fermented soybean and feather meal mixture to a starter feed is a feasible option to improve the growth performance of goat kids, without negatively impacting their health.

Beneficial effects of a calf starter versus forage on rumen development and bacteria populations in beef calves / Efeitos benéficos de um concentrado de bezerro versus forragem no desenvolvimento ruminal e na população de bactérias em bezerros

Rumen development depends on the intake of solid food that is fermented into volatile fatty acids that stimulate the development of the rumen papillae in calves. The starter feeding can promote the growth of papillae in the rumen and as a consequence an earlier weaning. We evaluated the effects of calf starter on ruminal development, and productive response of lactating bull calves raised for meat in the tropics. Twelve male Brahman × Swiss American cross beef calves from a dual-purpose system were randomly assigned two treatments with six animals per treatment: milk-fed calves + Taiwan grass (Pennisetum purpureum, MT) and MT + calf starter, (MTS). Feed intake and growth were measured at 7-day intervals throughout until 210 d of age. At 90 days old, three calves from each treatment were harvested, and fluid and ruminal tissues were collected from the cranial, ventral, dorsal, and dorsal blind ruminal sacs for measurements of many papillaes per cm2 (NP), papillae length (LP) and papillae width (WP). Ruminal bacterial genotype identification was determined by amplicon generation with the Illumina platform. Calf starter-improved weight (Live weight, LW) and average weight gain (ADG) and NP, but, LP and WP was similar in both treatments (p 0.05). In calves with starter feed treatment, we observed the bacteria Desulfonauticus autotrophicus sp. nov.that was not previously reported in ruminants. Use of calf starter showed benefit for calves with improved feed intake and rumen development because promoted a greater number of rumen papillae.(AU)

O desenvolvimento do rumen depende da ingestão de alimentos sólidos fermentados em ácidos graxos voláteis que estimulam o desenvolvimento das papilas de rúmen em bezerros. A alimentação inicial pode promover o crescimento das papilas no rúmen e, como consequência, um desmame mais cedo. Avaliamos os efeitos da concentrado de bezerros no desenvolvimento ruminal, e a resposta produtiva dos bezerros lactantes criados para carne nos trópicos. Doze bezerros de carne bovina cruza Brahman × suíço-americano machos de de um sistema de dupla finalidade foram aleatoriamente atribuídos a dois tratamentos, com seis animais por tratamento: bezerros alimentados com leite + grama taiwanesa (Pennisetumpurpureum, MT) e MT+ concentrado de bezerro, (MTS). A ingestão de ração e o crescimento foram medidos. Aos 90 dias de idade, três bezerros de cada tratamento foi amostrado, e foram coletados tecidos fluidos dos sacos ruminais cranianos, ventral, dorsal e dorsal cegos para medições do número de papilas por cm2 (NP), comprimento das papilas (LP) e largura da papila (PM). A identificação do genótipo das bactérias ruminais foi determinada pela geração de amplicon com a plataforma de ilumine. O concentrado de bezerro melhorou o peso (LW) e o ganho diário médio (AGD) e NP, mas, LP e WP foram semelhantes nos dois tratamentos. Bezerros em MTS uma bactéria não encontrada anteriormente em ruminantes Desulfonauticus autotrophicus sp. nov. foi detectado. O uso de concentrado de bezerro mostrou benefício para bezerros com melhor ingestão de ração e desenvolvimento de rúmen, pois promove um maior número de papilas de rúmen.(AU)

Qualidade de forragem de milho hidropônico cultivado em diferentes substratos de subproduto / Quality of hydroponic forage corn cultivated on different by-product substrates

O cultivo hidropônico de milho é uma alternativa eficiente, rápida e viável para períodos de escassez de alimentos; entretanto, ainda são poucas as informações sobre os parâmetros qualitativos e quantitativos da biomassa produzida, principalmente no que diz respeito aos substratos. Este trabalho teve como objetivo avaliar os aspectos produtivos e qualitativos do milho hidropônico para ração cultivado em diferentes substratos com um período de cultivo de 15 dias. Quatro substratos foram avaliados: 1) sementes de açaí inteiras fermentadas, 2) sementes de açaí trituradas, 3) bagaço de cana-de-açúcar e 4) feno de Tifton moído, com cinco repetições em delineamento de blocos ao acaso. A temperatura do substrato foi monitorada durante o período de produção. Após a colheita, no dia 15, foram avaliados o comprimento das raízes (RL), o comprimento da parte aérea, o teor de matéria seca da biomassa (BDM), o rendimento da biomassa seca, a produtividade da massa seca da forragem, a proteína bruta (PB) e o teor de cinzas. Não houve correlação entre período de crescimento e temperatura do substrato. O RL não foi afetado pelos substratos, o BDM foi menor no tratamento 3, o PB não foi influenciado e o teor de cinzas foi maior no tratamento 1. Em geral, o melhor desenvolvimento foi observado no tratamento 1 devido à ausência de distinção quanto aos parâmetros qualitativos (PB e cinzas) e maior granulometria das sementes inteiras de açaí que afetam a densidade de massa e aeração do substrato, permitindo maior rendimento de biomassa seca.

Hydroponic corn cultivation is an efficient, fast, and feasible alternative for periods of food scarcity; however, there is still little information on the qualitative and quantitative parameters of the produced biomass, especially with regard to substrates. This study aimed to evaluate the productive and qualitative aspects of hydroponic feed corn grown on different substrates with a cultivation period of 15 days. Four substrates were evaluated: 1) fermented whole açaí seeds, 2) crushed açaí seeds, 3) sugarcane bagasse, and 4) ground Tifton hay, with five replications under a randomized block design. Substrate temperature was monitored during the production period. After harvesting on day 15, roots length (RL), shoot length (SL), biomass dry matter content (BDM), dry biomass yield, forage dry mass productivity, crude protein (CP), and ash content were assessed. There was no correlation of growth period and substrate temperature. RL was not affected by substrates, BDM was lower in treatment 3, CP was not influenced, and ash content was higher in treatment 1. In general, the best development was observed in treatment 1 because of the absence of distinction regarding qualitative parameters (CP and ash) and higher granulometry of whole açaí seeds which affects mass density and substrate aeration, thus allowing higher dry biomass yield.

Quality of hydroponic forage corn cultivated on different by-product substrates / Qualidade de forragem de milho hidropônico cultivado em diferentes substratos de subproduto

Hydroponic corn cultivation is an efficient, fast, and feasible alternative for periods of food scarcity; however, there is still little information on the qualitative and quantitative parameters of the produced biomass, especially with regard to substrates. This study aimed to evaluate the productive and qualitative aspects of hydroponic feed corn grown on different substrates with a cultivation period of 15 days. Four substrates were evaluated: 1) fermented whole açaí seeds, 2) crushed açaí seeds, 3) sugarcane bagasse, and 4) ground Tifton hay, with five replications under a randomized block design. Substrate temperature was monitored during the production period. After harvesting on day 15, roots length (RL), shoot length (SL), biomass dry matter content (BDM), dry biomass yield, forage dry mass productivity, crude protein (CP), and ash content were assessed. There was no correlation of growth period and substrate temperature. RL was not affected by substrates, BDM was lower in treatment 3, CP was not influenced, and ash content was higher in treatment 1. In general, the best development was observed in treatment 1 because of the absence of distinction regarding qualitative parameters (CP and ash) and higher granulometry of whole açaí seeds which affects mass density and substrate aeration, thus allowing higher dry biomass yield.

O cultivo hidropônico de milho é uma alternativa eficiente, rápida e viável para períodos de escassez de alimentos; entretanto, ainda são poucas as informações sobre os parâmetros qualitativos e quantitativos da biomassa produzida, principalmente no que diz respeito aos substratos. Este trabalho teve como objetivo avaliar os aspectos produtivos e qualitativos do milho hidropônico para ração cultivado em diferentes substratos com um período de cultivo de 15 dias. Quatro substratos foram avaliados: 1) sementes de açaí inteiras fermentadas, 2) sementes de açaí trituradas, 3) bagaço de canade-açúcar e 4) feno de Tifton moído, com cinco repetições em delineamento de blocos ao acaso. A temperatura do substrato foi monitorada durante o período de produção. Após a colheita, no dia 15, foram avaliados o comprimento das raízes (RL), o comprimento da parte aérea, o teor de matéria seca da biomassa (BDM), o rendimento da biomassa seca, a produtividade da massa seca da forragem, a proteína bruta (PB) e o teor de cinzas. Não houve correlação entre período de crescimento e temperatura do substrato. O RL não foi afetado pelos substratos, o BDM foi menor no tratamento 3, o PB não foi influenciado e o teor de cinzas foi maior no tratamento 1. Em geral, o melhor desenvolvimento foi observado no tratamento 1 devido à ausência de distinção quanto aos parâmetros qualitativos (PB e cinzas) e maior granulometria das sementes inteiras de açaí que afetam a densidade de massa e aeração do substrato, permitindo maior rendimento de biomassa seca.

The response of laying hens fed fermented pineapple peel waste by indigenous microorganism from bamboo sprout / Resposta de galinhas poedeiras alimentadas com resíduos fermentados de casca de abacaxi por microorganismos indígenas do broto de bambu

The bromelain enzyme content in pineapple peel waste predicted to promote digestive tract health and a positive effect on egg quality. This research aimed to evaluate the use of fermented pineapple peel waste in laying hens diet on egg quality. A total of laying hens 200 birds with Isa Brown strain, egg production of 70%, The average egg weight and body weight were 58.58 g/egg, and 1.62 g/bird, respectively. A completely randomized design was used, 5 different levels of fermented pineapple peel waste as treatment (0, 5, 10, 15, and 20%), and all treatments were repeated 4 times. Egg shell thickness, egg shell strength, haugh unit, egg yolk fat, and egg yolk color were measured. The use of fermented pineapple peel waste in the diet of laying hens up to 20% highly significant (P 0.01) on egg yolk color, and did not negative effect (P>0.05) on egg shell thickness, egg shell strength, haugh unit, and egg yolk fat. Fermented pineapple peel waste can be used as much as 20% in laying hens diet without disturbing egg quality and improve egg yolk color.(AU)

O conteúdo da enzima bromelina nos resíduos de casca de abacaxi previa promover a saúde do trato digestivo e um efeito positivo na qualidade dos ovos. Esta pesquisa teve como objetivo avaliar o uso de resíduos de casca de abacaxi fermentado na dieta de galinhas poedeiras, investigando seu efeito na qualidade dos ovos. Um total de galinhas poedeiras 200 aves com cepa Isa Brown, 70% de produção de ovos. O peso médio dos ovos e o peso corporal foram 58,58 g / ovo e 1,62 g / ave, respectivamente. Utilizou-se um delineamento inteiramente casualizado, com 5 níveis diferentes de resíduos de casca de abacaxi fermentado como tratamento (0, 5, 10, 15 e 20%) e todos os tratamentos foram repetidos 4 vezes. A espessura da casca do ovo, a força da casca do ovo, a unidade de haugh, a gordura da gema do ovo e a cor da gema do ovo foram medidas. O uso de resíduos de casca de abacaxi fermentado na dieta de galinhas poedeiras de até 20% de alta significância (P 0,01) na cor da gema de ovo e não teve efeito negativo (P> 0,05) na espessura da casca do ovo, na força da casca do ovo, na unidade de haugh e gordura de gema de ovo. O desperdício fermentado de casca de abacaxi pode ser usado em até 20% na dieta de galinhas poedeiras, sem prejudicar a qualidade dos ovos e melhorar a cor da gema.(AU)

The response of laying hens fed fermented pineapple peel waste by indigenous microorganism from bamboo sprout / Resposta de galinhas poedeiras alimentadas com resíduos fermentados de casca de abacaxi por microorganismos indígenas do broto de bambu

The bromelain enzyme content in pineapple peel waste predicted to promote digestive tract health and a positive effect on egg quality. This research aimed to evaluate the use of fermented pineapple peel waste in laying hens diet on egg quality. A total of laying hens 200 birds with Isa Brown strain, egg production of 70%, The average egg weight and body weight were 58.58 g/egg, and 1.62 g/bird, respectively. A completely randomized design was used, 5 different levels of fermented pineapple peel waste as treatment (0, 5, 10, 15, and 20%), and all treatments were repeated 4 times. Egg shell thickness, egg shell strength, haugh unit, egg yolk fat, and egg yolk color were measured. The use of fermented pineapple peel waste in the diet of laying hens up to 20% highly significant (P 0.01) on egg yolk color, and did not negative effect (P>0.05) on egg shell thickness, egg shell strength, haugh unit, and egg yolk fat. Fermented pineapple peel waste can be used as much as 20% in laying hens diet without disturbing egg quality and improve egg yolk color.

O conteúdo da enzima bromelina nos resíduos de casca de abacaxi previa promover a saúde do trato digestivo e um efeito positivo na qualidade dos ovos. Esta pesquisa teve como objetivo avaliar o uso de resíduos de casca de abacaxi fermentado na dieta de galinhas poedeiras, investigando seu efeito na qualidade dos ovos. Um total de galinhas poedeiras 200 aves com cepa Isa Brown, 70% de produção de ovos. O peso médio dos ovos e o peso corporal foram 58,58 g / ovo e 1,62 g / ave, respectivamente. Utilizou-se um delineamento inteiramente casualizado, com 5 níveis diferentes de resíduos de casca de abacaxi fermentado como tratamento (0, 5, 10, 15 e 20%) e todos os tratamentos foram repetidos 4 vezes. A espessura da casca do ovo, a força da casca do ovo, a unidade de haugh, a gordura da gema do ovo e a cor da gema do ovo foram medidas. O uso de resíduos de casca de abacaxi fermentado na dieta de galinhas poedeiras de até 20% de alta significância (P 0,01) na cor da gema de ovo e não teve efeito negativo (P> 0,05) na espessura da casca do ovo, na força da casca do ovo, na unidade de haugh e gordura de gema de ovo. O desperdício fermentado de casca de abacaxi pode ser usado em até 20% na dieta de galinhas poedeiras, sem prejudicar a qualidade dos ovos e melhorar a cor da gema.

Effect of fermented soybean meal supplementation on some growth performance, blood chemical parameters, and fecal microflora of finishing pigs

This study evaluated the effect of fermented soybean meal (FSBM) supplementation on growth performance, meat quality, blood biochemical parameters, and fecal microflora of finishing pigs. Thirty-two crossbred pigs (Duroc × Landrance × Yorkshire) (66-day-old, 67.95±0.25 kg) were randomly allocated to two treatments and fed diets containing soybean meal and FSBM. The average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (FCR), blood biochemical parameters, and meat quality index were measured. At the end of experiment, the fecal microflora of finishing pigs was analyzed with 16S rDNA techniques. Results revealed that pigs fed FSBM had a greater ADG and lower cooking loss relative to control group (basal diets). Compared with the control group, the triglyceride content in the serum of the group fed FSBM increased significantly, and the creatinine content in the serum decreased notably. Fermented soybean meal enhanced the abundance of Bacteroidetes, Prevotellaceae, Bcteroidales, Bacteroidia, but inhibited the growth of Firmicutes, Clostridia, Clostridiales, and Ruminococcaceae in the intestine of pigs. Therefore, we can speculate that FSBM may play an important role in animal production. Dietary FSBM supplementation may be beneficial to some aspects of growth performance and the diversity of fecal microflora in finishing pigs.(AU)

Influence of supplementing Guinea grass with differently processed African yam bean on gas production and in vitro digestibility

This study evaluated the potential utilisation of African yam bean (AYB) seed as a supplement to Guinea grass on in vitro gas and methane (CH4) production, as well as the effect of processing on AYB nutritive value. In experiment 1, unprocessed AYB meal at 10, 15, 20, and 25% inclusion levels was added to Guinea grass substrate and evaluated for in vitro gas production, CH4, and in vitro organic matter digestibility (IVOMD). In experiment 2, the effect of soaked, boiled, toasted, and fermented AYB meal at 20% inclusion on in vitro fermentation was evaluated. In vitro gas production as well as in vitro organic matter digestibility of Guinea grass increased with AYB supplementation. The associative effect of Guinea grass with AYB showed an increase in gas and CH4 production. At 20% inclusion level, AYB processing methods did not affect the gas production, CH4, and IVOMD of the substrate. Fermentation improved the crude protein, iron, and zinc contents, reduced acid detergent fibre concentration but did not reduce the concentrations of alkaloid, total phenol, saponin, and trypsin inhibitors of AYB. Seed soaking for 48 h reduced the total phenol, tannin, oxalate, and phytate content, while seed boiling reduced the concentrations of alkaloid, total phenol, tannin, and trypsin inhibitors. Compared with the raw AYB, boiling is the most effective processing method to reduce the investigated phytochemicals, followed by soaking, toasting, and fermentation, in that order.(AU)

In vitro fermentation of the rations containing Morinda citrifolia L. (Noni) using two types of inoculum / Fermentação in vitro de rações contendo Morinda citrifolia L (Noni) com uso de dois tipos de inóculo

The effect of inclusion of Morinda citrifolia L. (Noni) in standardized diets of sheep on in vitro digestibility and parameters fermentation was examined using two different inoculums (ruminal liquid and sheep feces). To determine the nutrients in vitro digestibility coefficient (IVDC), two sheep were used as inoculum donors. The experimental design was factorial (4 × 2), with four diets containing different proportions of Noni and two types of inoculum. The different dietary proportions of Noni (0%, 8%, 16%, and 24%) did not affect the IVDC of dry matter (DM), organic matter (OM) and crude protein (CP) for both inoculums. The IVDC of neutral detergent fiber (NDF) differed significantly between the inoculums. After in vitro incubation for 24 h, the pH value of the fermented material differed between the different diets and between the inoculums. Moreover, the concentration of ammoniacal nitrogen was affected by the proportion of Noni and differed between the inoculums after incubation for 24 h. The inclusion of up to 24% of Noni in standardized rations for ruminants did not affect the IVDC of DM, OM, CP, and NDF. The use of feces as inoculum for in vitro fermentation produced lower IVDC values than those by ruminal liquid. The diet containing 8% of Noni and fermented with ruminal liquid produced higher pH values after 24 h of incubation. Taken together, the different dietary...(AU)

Avaliou-se a inclusão de Morinda citrofolia L (Noni) em rações balanceadas para ovinos sobre o coeficiente de digestibilidade in vitro (CDIV) dos nutrientes e os paramentos da fermentação in vitro com utilização de diferentes inóculos (líquido ruminal e fezes de ovinos). Para a determinação do CDIV dos nutrientes foram utilizados dois ovinos com peso corporal médio de 25 + 2,0 kg, como doadores de inóculo (líquido ruminal e fezes). O delineamento experimental foi o fatorial 4X2, com quatro teores de inclusão de Noni e dois tipos de inóculo para a determinação do CDIV dos nutrientes. As variáveis estudadas foram submetidas à análise de variância e para os teores de inclusão de Noni procedeu-se análise de regressão a 5% de probabilidade e para os diferentes inóculos foi realizado teste de Tukey a 5% de significância. Os diferentes teores de inclusão do Noni (0%; 8%; 16% e 24%) nas rações balanceadas os ovinos não alteraram (P > 0,05) os CDIV da MS; MO e PB para ambos os inóculos (líquido ruminal e fezes de ovinos). Foi observado que a utilização dos diferentes inóculos propiciou alteração (P < 0,05) no valor do CDIV da FDN. Os teores de 0%; 8%; 16% e 24% de Noni nas rações para ruminantes e os diferentes inóculos (líquido ruminal e fezes de ovinos) propiciaram alterações (P < 0,05) no valor de pH do conteúdo fermentado após a incubação in vitro de 24 horas. A inclusão de...(AU)

Effects of Different Types of Red Ginseng Marc and Fermented Red Koji Blend as Feed Additives on Blood Parameters and Egg Yolk Fatty Acid Profiles of Laying Hens

ABSTRACT The purpose of this study was to evaluate the effects of three different forms (powder, pellet, and coated pellet) of red ginseng marc (RGM) and fermented red koji (FRK) feed additive on the blood parameters and fatty acid (FA) profiles of laying hens, after two months of dietary supplementation. A number of 240 40-week-old Hy-line Brown laying hens were randomly designated to four dietary treatments, each with six replicates of 10 hens each, in a completely randomized design. The control group was fed a basal diet, and the other three treatments groups were fed 1% RGM powder mixed with FRK, pellets of 1% RGM with FRK, and coated pellets of 1% RGM with FRK. The powder and coated pellet diets contained the lowest total cholesterol and glucose levels, respectively, whereas the coated pellet diet contained the highest HDL cholesterol level, compared to the basal diet of the control group. For FA profiles, significant differences (p 0.05) were observed among the groups with regard to the percentages of myristoleic acid, palmitoleic acid, margaric acid, margaroleic acid, stearic acid, oleic acid, a-linolenic acid, eicosenoic acid, and docosahexaenoic acid. Moreover, addition of different forms of the RGM and FRK blend to the hens diets showed an increase (p 0.05) in the relative percentages of unsaturated fatty acids (UFA) and the UFA: SFA ratio and a decrease (p 0.05) in the relative percentages of saturated fatty acids (SFA) in the egg yolk, compared to the control group. In conclusion, dietary coated pellets of RGM and FRK as a feed additive blend had a beneficial effect on serum cholesterol and FA profiles in laying hens.

Evaluation of sample extracting methods of FCSM by Lactobacillus acidophilus based on a UPLC-Q-TOF-MS global metabolomics analysis

Abstract The study of metabolomics requires extracting as many metabolites as possible from a biological sample. This study aimed to determine the optimal method for the extraction of metabolites from solid-state fermented cottonseed meal (FCSM). The UPLC-Q-TOF-MS global metabolomics technology was used to detect the metabolites in FCSM, and the extraction quantity and extraction efficiency of seven different extraction methods, specifically the WA, 50MeOH, 50MeOHB, 50MeCNB, 80MeOHB, 80MeOH and AMF methods were evaluated. The results showed that the number of VIP metabolites extracted by AMF method are 196 and 184 in ESI+ and ESI- mode respectively, it is the largest number of all exacted methods; and the AMF methods also provided a higher extraction efficiency compared with the other methods, especially in indoleacrylic acid, dl-tryptophan and epicatechin (p 0.01). As a result, AMF/-4 °C method was identified as the best method for the extraction of metabolites from FCSM by Lactobacillus acidophilus. Our study establishes a technical basis for future metabolomics research of fermented feed.

Evaluation of sample extracting methods of FCSM by Lactobacillus acidophilus based on a UPLC-Q-TOF-MS global metabolomics analysis

The study of metabolomics requires extracting as many metabolites as possible from a biological sample. This study aimed to determine the optimal method for the extraction of metabolites from solid-state fermented cottonseed meal (FCSM). The UPLC-Q-TOF-MS global metabolomics technology was used to detect the metabolites in FCSM, and the extraction quantity and extraction efficiency of seven different extraction methods, specifically the WA, 50MeOH, 50MeOHB, 50MeCNB, 80MeOHB, 80MeOH and AMF methods were evaluated. The results showed that the number of VIP metabolites extracted by AMF method are 196 and 184 in ESI+ and ESI- mode respectively, it is the largest number of all exacted methods; and the AMF methods also provided a higher extraction efficiency compared with the other methods, especially in indoleacrylic acid, dl-tryptophan and epicatechin (p < 0.01). As a result, AMF/-4 °C method was identified as the best method for the extraction of metabolites from FCSM by Lactobacillus acidophilus. Our study establishes a technical basis for future metabolomics research of fermented feed.(AU)

Effects of Different Types of Red Ginseng Marc and Fermented Red Koji Blend as Feed Additives on Blood Parameters and Egg Yolk Fatty Acid Profiles of Laying Hens

The purpose of this study was to evaluate the effects of three different forms (powder, pellet, and coated pellet) of red ginseng marc (RGM) and fermented red koji (FRK) feed additive on the blood parameters and fatty acid (FA) profiles of laying hens, after two months of dietary supplementation. A number of 240 40-week-old Hy-line Brown laying hens were randomly designated to four dietary treatments, each with six replicates of 10 hens each, in a completely randomized design. The control group was fed a basal diet, and the other three treatments groups were fed 1% RGM powder mixed with FRK, pellets of 1% RGM with FRK, and coated pellets of 1% RGM with FRK. The powder and coated pellet diets contained the lowest total cholesterol and glucose levels, respectively, whereas the coated pellet diet contained the highest HDL cholesterol level, compared to the basal diet of the control group. For FA profiles, significant differences (p 0.05) were observed among the groups with regard to the percentages of myristoleic acid, palmitoleic acid, margaric acid, margaroleic acid, stearic acid, oleic acid, a-linolenic acid, eicosenoic acid, and docosahexaenoic acid. Moreover, addition of different forms of the RGM and FRK blend to the hens diets showed an increase (p 0.05) in the relative percentages of unsaturated fatty acids (UFA) and the UFA: SFA ratio and a decrease (p 0.05) in the relative percentages of saturated fatty acids (SFA) in the egg yolk, compared to the control group. In conclusion, dietary coated pellets of RGM and FRK as a feed additive blend had a beneficial effect on serum cholesterol and FA profiles in laying hens.(AU)

Effects of Different Types of Red Ginseng Marc and Fermented Red Koji Blend as Feed Additives on Blood Parameters and Egg Yolk Fatty Acid Profiles of Laying Hens

The purpose of this study was to evaluate the effects of three different forms (powder, pellet, and coated pellet) of red ginseng marc (RGM) and fermented red koji (FRK) feed additive on the blood parameters and fatty acid (FA) profiles of laying hens, after two months of dietary supplementation. A number of 240 40-week-old Hy-line Brown laying hens were randomly designated to four dietary treatments, each with six replicates of 10 hens each, in a completely randomized design. The control group was fed a basal diet, and the other three treatments groups were fed 1% RGM powder mixed with FRK, pellets of 1% RGM with FRK, and coated pellets of 1% RGM with FRK. The powder and coated pellet diets contained the lowest total cholesterol and glucose levels, respectively, whereas the coated pellet diet contained the highest HDL cholesterol level, compared to the basal diet of the control group. For FA profiles, significant differences (p 0.05) were observed among the groups with regard to the percentages of myristoleic acid, palmitoleic acid, margaric acid, margaroleic acid, stearic acid, oleic acid, a-linolenic acid, eicosenoic acid, and docosahexaenoic acid. Moreover, addition of different forms of the RGM and FRK blend to the hens diets showed an increase (p 0.05) in the relative percentages of unsaturated fatty acids (UFA) and the UFA: SFA ratio and a decrease (p 0.05) in the relative percentages of saturated fatty acids (SFA) in the egg yolk, compared to the control group. In conclusion, dietary coated pellets of RGM and FRK as a feed additive blend had a beneficial effect on serum cholesterol and FA profiles in laying hens.

Effect of broccoli residues fermented with probiotics on the growth performance and health status of broilers challenged with Clostridium perfringens

This study aimed at investigating the effects of broccoli residues fermented with probiotics (BF) on the growth performance, immunity, and gut health in broilers challenged with Clostridium perfringens (C. perfringens). A total of 600 broilers (one day old) were randomly allotted into five treatments with six replicates of 20 birds each and were reared until 42 days of age. The treatments included a positive control (PC, fed a basal diet and reared on uncontaminated litter), a negative control (NC, birds reared on litter contaminated with C. perfringens and fed a basal diet), and NC plus BF at 25, 50 or 75 g/kg of diet. The BF contained yeast 3.1 × 10 (7) cfu/g, lactic acid bacteria 9.5 × 10 (6) cfu/g and Bacillus subtilis 3.5 × 10 (6) cfu/g. Birds in the NC group showed lower (p<0.05) feed intake and body weight gain, whereas BF supplementation recovered (p<0.05) the growth performance to the levels of PC group. Dietary BF at 50and 75 g/kg reduced (p<0.05) broiler mortality. Similarly, compared to the NC group, BF increased (p<0.05) immune organ weights and serum immunoglobulins A, G, and M to the levels of PC group. The ileal populations of Escherichia coli and Gram-negative bacteria were decreased (p<0.05) by BF to the levels of PC, and C. perfringens was also decreased (p<0.05) by BF. The serum profiles of mono- and di-amine oxidase were decreased (p<0.05) by BF. BF at 75 g/kg reduced (p<0.05) monoamine oxidase compared with the other BF doses. The results suggest that broccoli residues fermented with probiotics can be a novel biological feed additive to protect the performance and health of broilers against C. perfringens infection.(AU)

Effect of broccoli residues fermented with probiotics on the growth performance and health status of broilers challenged with Clostridium perfringens

This study aimed at investigating the effects of broccoli residues fermented with probiotics (BF) on the growth performance, immunity, and gut health in broilers challenged with Clostridium perfringens (C. perfringens). A total of 600 broilers (one day old) were randomly allotted into five treatments with six replicates of 20 birds each and were reared until 42 days of age. The treatments included a positive control (PC, fed a basal diet and reared on uncontaminated litter), a negative control (NC, birds reared on litter contaminated with C. perfringens and fed a basal diet), and NC plus BF at 25, 50 or 75 g/kg of diet. The BF contained yeast 3.1 × 10 (7) cfu/g, lactic acid bacteria 9.5 × 10 (6) cfu/g and Bacillus subtilis 3.5 × 10 (6) cfu/g. Birds in the NC group showed lower (p<0.05) feed intake and body weight gain, whereas BF supplementation recovered (p<0.05) the growth performance to the levels of PC group. Dietary BF at 50and 75 g/kg reduced (p<0.05) broiler mortality. Similarly, compared to the NC group, BF increased (p<0.05) immune organ weights and serum immunoglobulins A, G, and M to the levels of PC group. The ileal populations of Escherichia coli and Gram-negative bacteria were decreased (p<0.05) by BF to the levels of PC, and C. perfringens was also decreased (p<0.05) by BF. The serum profiles of mono- and di-amine oxidase were decreased (p<0.05) by BF. BF at 75 g/kg reduced (p<0.05) monoamine oxidase compared with the other BF doses. The results suggest that broccoli residues fermented with probiotics can be a novel biological feed additive to protect the performance and health of broilers against C. perfringens infection.

Improving Nutrition Utilization and Meat Quality of Broiler Chickens Through Solid-State Fermentation of Agricultural By-Products by Aureobasidium Pullulans

A 35 d trial was conducted to evaluate the effects of dietary supplementation of co-fermented agricultural by-products, soybean hulls and Pleurotus eryngii stalk residue (PESR), with Aureobasidium pullulans on nutrients digestibility and meat quality in broilers fed on conventional corn-soybean meal basal diet. A total of 400 1-d-old Ross broilers were allotted to 4 dietary treatments with 4 replicate pens (25 birds per pen). Birds were fed the corn-soybean meal diets supplemented with 0% (CON), 0.5% fermented soybean hulls (0.5% FSBH), 0.5% fermented soybean hulls partially replaced with PESR (0.5% FSHP) and 1.0% FSHP. The broilers fed on the diet that contained fermented products had higher total tract apparent digestibility for hemicellulose than those on CON (p 0.05). Meanwhile, the birds fed on 0.5% FSHP also showed the lightest duodenum and jejunum weight among all groups. Compared with the CON, the inclusion of 1.0% FSHP in the diet significantly decreased ammonia N concentration in cecum followed by 0.5% FSHP and subsequent 0.5% FSBH group. Dietary supplementation of fermented products significantly improved WHC and increased protein content in meat as compared with the CON. Moreover, the fat content in meat decreased in the broilers that received 0.5% FSBH and 1.0% FSHP. In conclusion, dietary supplementation of co-fermented agricultural by-products, PESR and soybean meal, could improve the digestibility of hemicellulose and decrease the cecal ammonia N concentration; moreover, the increased WHC and protein level as well as decreased fat content in meat suggested the promising utilization of these low-cost materials as broiler feed additives.(AU)

Improving Nutrition Utilization and Meat Quality of Broiler Chickens Through Solid-State Fermentation of Agricultural By-Products by Aureobasidium Pullulans

A 35 d trial was conducted to evaluate the effects of dietary supplementation of co-fermented agricultural by-products, soybean hulls and Pleurotus eryngii stalk residue (PESR), with Aureobasidium pullulans on nutrients digestibility and meat quality in broilers fed on conventional corn-soybean meal basal diet. A total of 400 1-d-old Ross broilers were allotted to 4 dietary treatments with 4 replicate pens (25 birds per pen). Birds were fed the corn-soybean meal diets supplemented with 0% (CON), 0.5% fermented soybean hulls (0.5% FSBH), 0.5% fermented soybean hulls partially replaced with PESR (0.5% FSHP) and 1.0% FSHP. The broilers fed on the diet that contained fermented products had higher total tract apparent digestibility for hemicellulose than those on CON (p 0.05). Meanwhile, the birds fed on 0.5% FSHP also showed the lightest duodenum and jejunum weight among all groups. Compared with the CON, the inclusion of 1.0% FSHP in the diet significantly decreased ammonia N concentration in cecum followed by 0.5% FSHP and subsequent 0.5% FSBH group. Dietary supplementation of fermented products significantly improved WHC and increased protein content in meat as compared with the CON. Moreover, the fat content in meat decreased in the broilers that received 0.5% FSBH and 1.0% FSHP. In conclusion, dietary supplementation of co-fermented agricultural by-products, PESR and soybean meal, could improve the digestibility of hemicellulose and decrease the cecal ammonia N concentration; moreover, the increased WHC and protein level as well as decreased fat content in meat suggested the promising utilization of these low-cost materials as broiler feed additives.

Typology of dairy production systems from West Parana State based on production indices and feed used / Tipologia de sistemas de produção leiteiros do Oeste do Estado do Paraná baseada nos índices produtivos e nos alimentos utilizados

The dairy industry is characterised by a wide diversity of production systems. In this diverse environment, some of the difficulties can be minimised through the grouping of production units through the study of typology. The objective was to characterise the systems of milk production from West Parana State by considering production rates, area, power, facilities, and marketing of milk. To this end, 735 semi-structured interviews with dairy farmers were performed by using a questionnaire for management practices and feed that is used on the farms. To obtain the explanatory variables used for principal component analysis (PCA) and hierarchical cluster analysis (HCA), a homogeneous division of 735 farmers in five groups was used. Ten per cent of producers were randomly selected for the performance of field visits to the properties, where a new questionnaire was applied with specific points regarding feeding management on the property, and feed was collected to determine its composition. The collected data were tabulated and feed was ranked based on the classification proposed by the NRC (2001). Analyses of feed were carried out in the Laboratory of Animal Nutrition of Unioeste. The West Parana State has five groups of dairy production systems. There is great variability in the fresh forages that are used, but maize silage is the main forage utilised for fermented feed, and...(AU)

A pecuária leiteira é caracterizada por ampla diversidade de sistemas de produção. Nesse ambiente diverso, algumas das dificuldades podem ser minimizadas por meio do agrupamento das unidades de produção através do estudo da tipologia. Objetivou-se caracterizar os sistemas de produção de leite do Oeste do Estado do Paraná considerando os índices de produção, área, alimentação, instalações e comercialização do leite. Para tal, foram realizadas 735 entrevistas semiestruturadas, com produtores de leite, utilizando um questionário guia referente a práticas de manejo e dos alimentos utilizados nas propriedades. Para a obtenção das variáveis explicativas usou-se a análise de componentes principais (ACP) e análise de clusters hierárquicos (CHA), obtendo-se a divisão homogênea dos 735 produtores em cinco grupos. Destes foram selecionados ao acaso 10% dos produtores cada para a realização das visitas a campo nas propriedades, aplicando-se um novo questionário que contemplava pontos específicos do manejo alimentar utilizado na propriedade, bem como a coleta de alimentos para determinação da sua composição. Os dados coletados foram tabulados e os alimentos classificados com base na classificação dos alimentos proposta pelo NRC (2001). As análises dos alimentos foram realizadas no Laboratório de Nutrição Animal da Unioeste. O Oeste do Estado do Paraná possui cinco grupos de sistemas de...(AU)

Typology of dairy production systems from West Parana State based on production indices and feed used / Tipologia de sistemas de produção leiteiros do Oeste do Estado do Paraná baseada nos índices produtivos e nos alimentos utilizados

The dairy industry is characterised by a wide diversity of production systems. In this diverse environment, some of the difficulties can be minimised through the grouping of production units through the study of typology. The objective was to characterise the systems of milk production from West Parana State by considering production rates, area, power, facilities, and marketing of milk. To this end, 735 semi-structured interviews with dairy farmers were performed by using a questionnaire for management practices and feed that is used on the farms. To obtain the explanatory variables used for principal component analysis (PCA) and hierarchical cluster analysis (HCA), a homogeneous division of 735 farmers in five groups was used. Ten per cent of producers were randomly selected for the performance of field visits to the properties, where a new questionnaire was applied with specific points regarding feeding management on the property, and feed was collected to determine its composition. The collected data were tabulated and feed was ranked based on the classification proposed by the NRC (2001). Analyses of feed were carried out in the Laboratory of Animal Nutrition of Unioeste. The West Parana State has five groups of dairy production systems. There is great variability in the fresh forages that are used, but maize silage is the main forage utilised for fermented feed, and...

A pecuária leiteira é caracterizada por ampla diversidade de sistemas de produção. Nesse ambiente diverso, algumas das dificuldades podem ser minimizadas por meio do agrupamento das unidades de produção através do estudo da tipologia. Objetivou-se caracterizar os sistemas de produção de leite do Oeste do Estado do Paraná considerando os índices de produção, área, alimentação, instalações e comercialização do leite. Para tal, foram realizadas 735 entrevistas semiestruturadas, com produtores de leite, utilizando um questionário guia referente a práticas de manejo e dos alimentos utilizados nas propriedades. Para a obtenção das variáveis explicativas usou-se a análise de componentes principais (ACP) e análise de clusters hierárquicos (CHA), obtendo-se a divisão homogênea dos 735 produtores em cinco grupos. Destes foram selecionados ao acaso 10% dos produtores cada para a realização das visitas a campo nas propriedades, aplicando-se um novo questionário que contemplava pontos específicos do manejo alimentar utilizado na propriedade, bem como a coleta de alimentos para determinação da sua composição. Os dados coletados foram tabulados e os alimentos classificados com base na classificação dos alimentos proposta pelo NRC (2001). As análises dos alimentos foram realizadas no Laboratório de Nutrição Animal da Unioeste. O Oeste do Estado do Paraná possui cinco grupos de sistemas de...