Resumo
Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.
Assuntos
Animais , Análise do Sêmen , Crioprotetores/análise , Espermatozoides/citologia , Glucose/análise , Criopreservação , Peixes/classificaçãoResumo
Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.(AU)
Assuntos
Animais , Glucose/análise , Análise do Sêmen , Espermatozoides/citologia , Crioprotetores/análise , Peixes/classificação , CriopreservaçãoResumo
The present paper describes haematological characteristics of jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae), reared in captivity. The average values of red blood cells count, hematocrit, hemoglobin rate, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and defense blood cells (leukocytes and thrombocytes), were determined. Identification and morphology of organic defense blood cells were reported. Thrombocytes and lymphocytes were the most frequent cells in slides blood of R. quelen. Linear regression analysis showed negative correlation between thrombocytes and lymphocytes percentual. The present results furnish normal blood values of R. quelen reared intensively, that assist in comparison to the other culture conditions.
As características hematológicas do jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae), oriundo de cativeiro foram estudadas. Determinou-se o valor médio da contagem de eritrócitos, taxa de hemoglobina, hematócrito, volume corpuscular médio (VCM), concentração de hemoglobina corpuscular média (CHCM) e distribuição percentual de células sangüíneas de defesa orgânica. As células sangüíneas de defesa orgânica (leucócitos e trombócitos) foram identificadas e caracterizadas morfologicamente. Trombócitos e linfócitos foram as células sangüíneas de defesa orgânica mais freqüente nas extensões sangüíneas do R. quelen. A análise de regressão mostrou correlação linear negativa entre o percentual de trombócitos e linfócitos. Os resultados deste estudo fornecem valores sangüíneos normais em R. quelen, em cultivo intensivo, que poderão servir de comparação com dados dessa espécie em outras situações de cultivo.
Resumo
The present paper describes haematological characteristics of jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae), reared in captivity. The average values of red blood cells count, hematocrit, hemoglobin rate, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) and defense blood cells (leukocytes and thrombocytes), were determined. Identification and morphology of organic defense blood cells were reported. Thrombocytes and lymphocytes were the most frequent cells in slides blood of R. quelen. Linear regression analysis showed negative correlation between thrombocytes and lymphocytes percentual. The present results furnish normal blood values of R. quelen reared intensively, that assist in comparison to the other culture conditions.
As características hematológicas do jundiá Rhamdia quelen Quoy & Gaimard, 1824 (Osteichthyes: Pimelodidae), oriundo de cativeiro foram estudadas. Determinou-se o valor médio da contagem de eritrócitos, taxa de hemoglobina, hematócrito, volume corpuscular médio (VCM), concentração de hemoglobina corpuscular média (CHCM) e distribuição percentual de células sangüíneas de defesa orgânica. As células sangüíneas de defesa orgânica (leucócitos e trombócitos) foram identificadas e caracterizadas morfologicamente. Trombócitos e linfócitos foram as células sangüíneas de defesa orgânica mais freqüente nas extensões sangüíneas do R. quelen. A análise de regressão mostrou correlação linear negativa entre o percentual de trombócitos e linfócitos. Os resultados deste estudo fornecem valores sangüíneos normais em R. quelen, em cultivo intensivo, que poderão servir de comparação com dados dessa espécie em outras situações de cultivo.