Intake and apparent nutrient digestibility, ruminal parameters, and nitrogen balance of lambs fed with corn and forage-free diets with sodium bicarbonate / Consumo e digestibilidade aparente dos nutrientes, parâmetros ruminais e balanço de N de cordeiros confinados recebendo dietas com milho e sem forragem com bicarbonato de sódio

This study evaluated forage-free diets supplemented with sodium bicarbonate (SB) based on ground flint corn on intake, apparent nutrient digestibility, ruminal parameters, and N balance. Four rumen-cannulatedDorper × Santa Inês lambs, with a body weight of 43.4 ± 0.85 kg (mean ± standard deviation) were used. The experimental design was a 4×4 Latin square. Each experimental period lasted 21 days. Experimental diets were: positive control, a diet without SB containing 400 g/kg DM of soybean hulls (CONT), and three diets based on ground flint corn, containing 10 (10SB), 20 (20SB) or 30 (30SB) g/kg DM of SB. Intake of neutral and acid detergent fiber was greater for CONT (P < 0.01) when compared to SB. Intake and apparent nutrient digestibility of dry and organic matter, ether extract, and crude protein did not differ. Intake (P = 0.01) and digestibility (P < 0.01) of non-fiber carbohydrates were greater for SB diets and neutral detergent fiber digestibility was greater (P = 0.03) for CONT. The total digestible nutrients and metabolizable energy were greater for SB (P = 0.04). Rumen concentrations of acetate (P < 0.01), isobutyrate (P < 0.01), butyrate (P < 0.01), isovalerate (P < 0.01), and total short-chain fatty acids (P < 0.01) were greater for CONT. Ruminal pH showed a quadratic (P = 0.05) response to SB levels, with greater values for 20SB. Sodium bicarbonate was efficient in increasing ruminal pH at the level of 20 g/kg DM without interfering with the intake and apparent nutrient digestibility characteristics.

O presente estudo avaliou a suplementação de bicarbonato de sódio (BS) em dietas sem forragem à base de milho moído sobre o consumo e a digestibilidade aparente dos nutrientes, os parâmetros ruminais e o balanço de N. Foram utilizados quatro cordeiros Dorper × Santa Inês, com um peso corporal inicial de 43,4 ± 0,85 kg (média ± desvio padrão). O delineamento experimental foi quadrado latino 4×4. Cada período experimental durou 21 dias. As dietas experimentais foram: controle positivo, sem BS, contendo 400 g/kg de MS de casca de soja (CONT) e três dietas à base de milho moído, sem casca de soja, contendo 10 (10BS), 20 (20BS) ou 30 (30BS) g/kg de MS de BS. O consumo de fibra em detergente neutro e ácido foi maior (P < 0,01)para o CONT quando comparado com BS. O consumo e a digestibilidade aparente da matéria seca, matéria orgânica, extrato etéreo e proteína bruta não diferiram entre os tratamentos. O consumo (P = 0,01) e digestibilidade (P < 0,01) dos carboidratos não fibrosos foram superiores para as dietas contendo BS e a digestibilidade da fibra em detergente neutro foi maior para o CONT. Os teores de nutrientes digestivos totais e energia metabolizável foram superiores para as dietas contendo BS (P = 0,04). A concentração ruminal de acetato (P < 0,01), isobutirato (P < 0,01), butirato (P < 0,01), isovalerato (P < 0,01) e ácidos graxos totais de cadeia curta (P < 0,01) foram mais elevadas para o CONT. O pHruminal apresentou resposta quadrática (P = 0,05) em relação aos níveis de BS, sendo observado maior pH para os animais alimentados com a dieta 20BS. O BS foi eficiente em aumentar o pHruminal no teor de 20 g/kg MS sem interferir no consumo e na digestibilidade aparente dos nutrientes. Portanto, 20 g/kg de MS é a dose indicada de BS em dietas sem forragem para terminação de cordeiros em confinamento.

Inhibition of mycelial growth, conidial germination, and Botrytis cinerea Pers: Fr colonization in begonia with biocompatible products

This study evaluated the effects of potassium and sodium carbonate and bicarbonate, Bacillus subtilis (Cohn, 1872) QST-713, Bacillus pumilus (Meyer & Gottheil, 1901) QST-2808, and crude and roasted coffee oils on the inhibition of mycelial growth and conidial germination in Botrytis cinerea Pers.: Fr and the colonization of begonia (Begonia elatior Hort. ex Steud) leaf discs by B. cinerea inoculated before, simultaneously and after with these alternative products. The assays were carried out using the Baladin begonia cultivar. The inhibition of B. cinerea mycelial growth and conidial germination was proportional to increases in the concentration of all the products. The inhibition of conidial germination was directly proportional to the concentrations of B. pumilus QST-2808 and B. subtilis QST-713. Coffee oils were less efficient in inhibiting germination than the other products. The crude and roasted coffee oils, potassium and sodium carbonates and bicarbonates, and B. pumilus and B. subtilis sprayed 24 h before, simultaneously, or 24 h after pathogen inoculation inhibited the colonization of begonia leaf discs by B. cinerea. The positive results for the suppression of B. cinerea by the alternative products tested herein merit scrutiny. There is a pressing need to evaluate these products in the management of gray mold, as the severity of this disease is usually high under favorable conditions in greenhouses.

Use of enteral electrolyte solutions with different sodium acetate concentrations in weaned foals: the effects on acid-base balance, blood glucose, lactate, and urine ph / Utilização de soluções eletrolíticas enterais com diferentes concentrações de acetato em potros desmamados: efeitos sobre o equilíbrio ácido base, concentrações plasmáticas de glicose e lactato e pH urinário

The effects of acetate as an alkalinizing agent in maintenance enteral electrolyte solutions administered by nasogastric route in a continuous flow have not been previously described in weaned foals. This is the second part of a study that evaluated the effects of two electrolyte solutions of enteral therapy fluid in weaned foals. In this part, will be considered the effects of enteral electrolyte solutions containing different acetate concentrations on acid-base balance, blood glucose, lactate and urine pH of weaned foals. This was a controlled trial in a cross-over design performed in six foals with a mean age of 7.3 ± 1.4 months. After 12 h of water and food deprivation, each animal received the following two treatments by nasogastric route in a continuous flow of 15 ml/kg/h during 12 h: HighAcetate (acetate 52 mmol/l) and LowAcetate (acetate 22.6 mmol/l). The HighAcetate treatment was effective in generating a slight increase in blood pH, blood bicarbonate concentration, base excess and urinary pH.

Os efeitos da utilização de acetato como um agente alcalinizante em soluções eletrolíticas enterais de manutenção administradas por sonda nasogástrica em fluxo contínuo não foram descritos em potros desmamados. O presente estudo teve como objetivo avaliar os efeitos de soluções eletrolíticas enterais contendo diferentes concentrações de acetato sobre o equilíbrio ácido base, concentrações plasmáticas de glicose e lactato, e pH urinário de potros desmamados. Este trabalho foi um estudo controlado em um delineamento cross-over (6X2) utilizando seis potros com idade média de 7.3 ± 1.4 meses. Após 12h de restrição hídrica e alimentar, cada animal recebeu os dois tratamentos por via nasogástrica em fluxo contínuo de 15 ml/kg/h durante 12h: HighAcetate (52 mmol/l) e LowAcetate (22.6 mmol/l). O grupo HighAcetate foi eficaz em gerar um pequeno aumento no pH sanguíneo, concentração de bicarbonato sanguíneo, excesso de base e pH urinário.

Clinical assessment of acid-base balance in Netherland Dwarf rabbit / Avaliação clínica do equilíbrio ácido-básico em coelhos Anã Holandês

Pet rabbits have increased their popularity in a lot of countries. However, most of the laboratory profiles in rabbit medicine come from the observations made in rabbit as biomodels or meat production. So that further researches are necessary to obtain reference values for hematology and biochemical profiles in pet rabbits and the different breeds, especially, in relation to acid-base balance. The aim of this report was to offer the mean values of the main parameters connected with acid-base profile in Netherland Dwarf breed. Thirty-five healthy rabbits (15 males and 20 females) were studied. Venous blood sample from lateral saphenous vein was analyzed to measure: haematocrit, haemoglobin, blood urea nitrogen, glucose, blood pH, partial pressure of CO2 (pCO2), total CO2, ions bicarbonate, chloride, sodium, potassium, base excess and anion Gap. Results showed a shorter range that those reported by different researchers. Moreover, differences between genders were showed in pCO2, its values were higher in males. It may be associated with a greater cellular metabolism. Values obtained in this research should be taken into account by veterinary clinicians for this breed in their clinical assessments. Besides, these values provide new results in parameters with few reference values.

A popularidade de coelhos como animais de estimação aumentou em muitos países. No entanto, a maioria dos perfis de laboratório em medicina de coelhos advém das observações de biomodelos animais ou da produção de carne. Assim, são necessárias pesquisas adicionais para obter valores de referência para hematologia e perfis bioquímicos em coelhos de estimação, e das diferentes raças, especialmente, em relação ao equilíbrio ácido-base. O objetivo deste relatório foi oferecer os valores médios dos principais parâmetros ligados ao perfil ácido-base na raça Anã Holandês. Trinta e cinco coelhos saudáveis ​​(15 machos e 20 fêmeas) foram estudados. A amostra de sangue venoso da veia safena lateral foi analisada para mensuração: hematócrito, hemoglobina, nitrogênio ureico sanguíneo, glicose, pH sanguíneo, pressão parcial de CO2 (pCO2), CO2 total, íons bicarbonato, cloreto, sódio, potássio, excesso de base e ânion Gap. Os resultados apresentaram um intervalo menor do que aqueles relatados por diferentes pesquisadores. Além disso, as diferenças entre os gêneros foram mostradas na pCO2, seus valores foram maiores no sexo masculino. Pode estar associado a um maior metabolismo celular. Os valores obtidos nesta pesquisa devem ser levados em consideração pelos clínicos veterinários para esta raça em suas avaliações clínicas. Além disso, esses valores fornecem novos resultados em parâmetros com poucos valores de referencia.

Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations / Perfil respiratório e histopatologia das brânquias de Carassius auratus expostas a diferentes concentrações de salinidades

The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-¹) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-¹ NaCl was 4 h, and 5 min at a concentration of 25 g L-¹. The mortality of fish with 15g L-¹ NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-¹ of NaCl. At concentrations of 20 and 25 g L-¹, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-¹ of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-¹ of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.

O objetivo deste estudo foi avaliar a tolerância crônica de Carassius auratus à salinidade e os efeitos sobre os parâmetros sanguíneos, morfologia branquial e sobrevivência. No primeiro teste, foram utilizadas nove concentrações de NaCl (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, e 25 g L-¹) e nove repetições por 96 h. A sobrevivência dos peixes submetidos a até 15 g L-¹ de NaCl foi de 04h00, sendo 00h05 na concentração de 25 g L-¹ de NaCl. A mortalidade dos peixes com 15 g foi de 100%. As análises morfológicas das brânquias mostraram hiperplasia das células revestidas no espaço interlamelar e hipersecreção de muco em peixes expostos a uma concentração de 10 g L-¹ de NaCl. Nas concentrações de 20 e 25 g L-¹, observou-se que a colágeno de suporte perdeu a estrutura das células da mucosa, alterando as lamelas secundárias. Em um segundo experimento, um delineamento inteiramente casualizado foi utilizado com dois tratamentos (0 e 5g L-¹ de NaCl) e quatro repetições com nove peixes por 30 L em 21 dias. Amostras de sangue e brânquias dos peixes foram coletadas e observou-se que os peixes apresentaram diminuição nas concentrações de bicarbonato (NaHCO3) no sangue, indicando hipernatremia. Conclui-se que a exposição aguda de C. auratus ao cloreto de sódio (NaCl) deve ser de no máximo 10 g L-¹, levando à perda de desempenho e/ou mortalidade dos animais. A exposição crônica a 5 g L-¹ de NaCl promove acidemia, desequilíbrio iônico e alterações patológicas nas brânquias, por isso não é recomendado.

Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations / Perfil respiratório e histopatologia das brânquias de Carassius auratus expostas a diferentes concentrações de salinidades

The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-¹) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-¹ NaCl was 4 h, and 5 min at a concentration of 25 g L-¹. The mortality of fish with 15g L-¹ NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-¹ of NaCl. At concentrations of 20 and 25 g L-¹, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-¹ of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-¹ of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.(AU)

O objetivo deste estudo foi avaliar a tolerância crônica de Carassius auratus à salinidade e os efeitos sobre os parâmetros sanguíneos, morfologia branquial e sobrevivência. No primeiro teste, foram utilizadas nove concentrações de NaCl (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, e 25 g L-¹) e nove repetições por 96 h. A sobrevivência dos peixes submetidos a até 15 g L-¹ de NaCl foi de 04h00, sendo 00h05 na concentração de 25 g L-¹ de NaCl. A mortalidade dos peixes com 15 g foi de 100%. As análises morfológicas das brânquias mostraram hiperplasia das células revestidas no espaço interlamelar e hipersecreção de muco em peixes expostos a uma concentração de 10 g L-¹ de NaCl. Nas concentrações de 20 e 25 g L-¹, observou-se que a colágeno de suporte perdeu a estrutura das células da mucosa, alterando as lamelas secundárias. Em um segundo experimento, um delineamento inteiramente casualizado foi utilizado com dois tratamentos (0 e 5g L-¹ de NaCl) e quatro repetições com nove peixes por 30 L em 21 dias. Amostras de sangue e brânquias dos peixes foram coletadas e observou-se que os peixes apresentaram diminuição nas concentrações de bicarbonato (NaHCO3) no sangue, indicando hipernatremia. Conclui-se que a exposição aguda de C. auratus ao cloreto de sódio (NaCl) deve ser de no máximo 10 g L-¹, levando à perda de desempenho e/ou mortalidade dos animais. A exposição crônica a 5 g L-¹ de NaCl promove acidemia, desequilíbrio iônico e alterações patológicas nas brânquias, por isso não é recomendado.(AU)

Clinical, hematological, blood gasometric and electrolytic changes in dogs receiving whole blood transfusions

Blood transfusion is a tool capable of saving lives. Patients undergoing blood transfusion usually present several alterations in the acid-base and electrolyte balance, aggravating the condition of critically ill patients. Some studies have demonstrated haematological alterations in certain species that received whole blood transfusions, however, few studies have evaluated acid base and electrolyte changes in dogs undergoing whole blood haemotherapy. The aim of this study was to analyze clinical, hematological, blood gas and electrolyte changes in anemic dogs after whole blood transfusion. Twenty nine dogs transfused due to anemia were enrolled in the study. Donors blood was collected in a transfusion bag containing citrate phosphate adenine dextrose and stored up to 24 h. Blood collections and evaluations were made before and 24 h after the transfusion. Data distribution normality was tested by the Shapiro Wilk Test. The means of the variables were compared by paired t-test. It was observed an increase in diastolic blood pressure and a reduction in heart rate (P < 0.05). There was a not significant increase in systolic blood pressure, temperature, and a reduction in respiratory rate per minute. Erythrocyte, haemoglobin and haematocrit averages were significantly increased after blood transfusion (P < 0.05). It was observed a reduction in the mean values of pH (P < 0.05), potassium (P > 0.05) and ionized calcium (P > 0.05) and an increase in the mean partial pressure of carbon dioxide (pCO2 ) (P < 0.001), bicarbonate (P > 0.05) and sodium (P < 0.05). The probable cause of anemia was monocytic ehrlichiosis (14/29), visceral leishmaniasis (1/29), babesiosis (1/29), co-infection of Ehrlichia canis and Leishmania infantum (2/29), co-infection of E. canis and Babesia vogeli (1/29). It was not possible to determine the etiology of the anemia in ten dogs. Heart rate significantly reduced after transfusion, probably because of the increase in hematocrit, hemoglobin and erythrocyte values. It may be justified by the displacement of extravascular fluid to the intravascular space. Mean values of systolic blood pressure were slightly elevated before transfusion and remained elevated afterwards, while diastolic and mean arterial pressure increased significantly after transfusion. These changes may be due to the morbid condition and may be influenced by many other factors. Haematocrit, haemoglobin and erythrocyte values increased significantly after transfusion, according to what was observed in other studies. The significant reduction in pH and increase in pCO2 reflects the compensatory mechanism for metabolic acidosis to increase ventilation, leading to pCO2 reduction and changes in pH. The reduction in pH due to the contact of the collected blood with conservative solutions is one of the main changes thar occurs during blood storage. It was described significantly lower pH in dogs' whole blood samples stored for more than 24 h in vacutainer plastic containing CPDA-1. We may assume there was no intense pH reduction in the present study because the bags were stored for up to 24 h. Although not statistically significant, the increase of pO2 mean reflects the improvement of tissue oxygen perfusion. It was observed a significant increase in sodium ions. The mean sodium ion concentration before transfusion was very close to the maximum reference value. Hyperkalaemia was not observed, nor was there significant reduction of potassium ions after transfusion. Several studies report hyperkalaemia and transfusion-associated cardiac arrests in humans, associated with infusion of large volumes of blood. Whole blood transfusion increased erythrogram values and did not negatively affect the electrolyte or acid-base status, representing a safe and useful tool in the intensive care of small animals.(AU)

Clinical assessment of acid-base balance in Netherland Dwarf rabbit / Avaliação clínica do equilíbrio ácido-básico em coelhos Anã Holandês

Pet rabbits have increased their popularity in a lot of countries. However, most of the laboratory profiles in rabbit medicine come from the observations made in rabbit as biomodels or meat production. So that further researches are necessary to obtain reference values for hematology and biochemical profiles in pet rabbits and the different breeds, especially, in relation to acid-base balance. The aim of this report was to offer the mean values of the main parameters connected with acid-base profile in Netherland Dwarf breed. Thirty-five healthy rabbits (15 males and 20 females) were studied. Venous blood sample from lateral saphenous vein was analyzed to measure: haematocrit, haemoglobin, blood urea nitrogen, glucose, blood pH, partial pressure of CO2 (pCO2), total CO2, ions bicarbonate, chloride, sodium, potassium, base excess and anion Gap. Results showed a shorter range that those reported by different researchers. Moreover, differences between genders were showed in pCO2, its values were higher in males. It may be associated with a greater cellular metabolism. Values obtained in this research should be taken into account by veterinary clinicians for this breed in their clinical assessments. Besides, these values provide new results in parameters with few reference values.(AU)

A popularidade de coelhos como animais de estimação aumentou em muitos países. No entanto, a maioria dos perfis de laboratório em medicina de coelhos advém das observações de biomodelos animais ou da produção de carne. Assim, são necessárias pesquisas adicionais para obter valores de referência para hematologia e perfis bioquímicos em coelhos de estimação, e das diferentes raças, especialmente, em relação ao equilíbrio ácido-base. O objetivo deste relatório foi oferecer os valores médios dos principais parâmetros ligados ao perfil ácido-base na raça Anã Holandês. Trinta e cinco coelhos saudáveis (15 machos e 20 fêmeas) foram estudados. A amostra de sangue venoso da veia safena lateral foi analisada para mensuração: hematócrito, hemoglobina, nitrogênio ureico sanguíneo, glicose, pH sanguíneo, pressão parcial de CO2 (pCO2), CO2 total, íons bicarbonato, cloreto, sódio, potássio, excesso de base e ânion Gap. Os resultados apresentaram um intervalo menor do que aqueles relatados por diferentes pesquisadores. Além disso, as diferenças entre os gêneros foram mostradas na pCO2, seus valores foram maiores no sexo masculino. Pode estar associado a um maior metabolismo celular. Os valores obtidos nesta pesquisa devem ser levados em consideração pelos clínicos veterinários para esta raça em suas avaliações clínicas. Além disso, esses valores fornecem novos resultados em parâmetros com poucos valores de referencia.(AU)

The Effects of Sodium Chloride versus Sodium Bicarbonate at the Same Dietary Sodium Concentration on Efficacy of Dietary Phytase and a Carbohydrase-Protease Cocktail in Broilers

A trial was conducted to determine the effect of phytase (PHY) or a carbohydrase/protease cocktail (CPX) on broilers fed diets with two different levels of chloride (0.28% or 0.43%) created by altering dietary salt (NaCl) and sodium bicarbonate (NaHCO3). There were 6 combination dietary treatments (3 enzyme x 2 NaCl treatments) applied to 4 replicate pens. The treatments were as follows: Control diet (CON), CON+PHY and CON+CPX, with 0.5% or 0.25% NaCl. The 0.25% NaCl versions contained 0.35% sodium bicarbonate. The 0.5% salt versions had no sodium bicarbonate. Replicate pen BW, and feed consumption (FC) were measured at 1, 14, and 35 d, and mortality was weighed daily for feed conversion ratio (FCR) calculations. Feed consumption at 14 d tended to be lower (p 0.10) for CON+CPX diets compared to CON and CON+PHY diets. The birds fed CON+CPX diet consumed less feed but exhibited improved FCR in the presence of 0.5% NaCl at 14 d. The birds fed the CON, and CON+PHY diets exhibited higher BW at 14 d (p 0.05) and 35 d (p 0.01) of age than did CON+CPX birds. From 15 d to 35 d, birds fed the CON+CPX diet exhibited poorer BW gain (BWG) in the presence of 0.25% NaCl (p 0.05). In conclusion, Cl, as NaCl versus NaH2CO3, could affect CPX but not PHY feed enzyme function in broilers. Further, it may be suggested that certain feed enzymes may be best utilized at later broiler ages rather than in initial feeds.(AU)

The Effects of Sodium Chloride versus Sodium Bicarbonate at the Same Dietary Sodium Concentration on Efficacy of Dietary Phytase and a Carbohydrase-Protease Cocktail in Broilers

A trial was conducted to determine the effect of phytase (PHY) or a carbohydrase/protease cocktail (CPX) on broilers fed diets with two different levels of chloride (0.28% or 0.43%) created by altering dietary salt (NaCl) and sodium bicarbonate (NaHCO3). There were 6 combination dietary treatments (3 enzyme x 2 NaCl treatments) applied to 4 replicate pens. The treatments were as follows: Control diet (CON), CON+PHY and CON+CPX, with 0.5% or 0.25% NaCl. The 0.25% NaCl versions contained 0.35% sodium bicarbonate. The 0.5% salt versions had no sodium bicarbonate. Replicate pen BW, and feed consumption (FC) were measured at 1, 14, and 35 d, and mortality was weighed daily for feed conversion ratio (FCR) calculations. Feed consumption at 14 d tended to be lower (p 0.10) for CON+CPX diets compared to CON and CON+PHY diets. The birds fed CON+CPX diet consumed less feed but exhibited improved FCR in the presence of 0.5% NaCl at 14 d. The birds fed the CON, and CON+PHY diets exhibited higher BW at 14 d (p 0.05) and 35 d (p 0.01) of age than did CON+CPX birds. From 15 d to 35 d, birds fed the CON+CPX diet exhibited poorer BW gain (BWG) in the presence of 0.25% NaCl (p 0.05). In conclusion, Cl, as NaCl versus NaH2CO3, could affect CPX but not PHY feed enzyme function in broilers. Further, it may be suggested that certain feed enzymes may be best utilized at later broiler ages rather than in initial feeds.

Clinical assessment of acid-base balance in Netherland Dwarf rabbit

Abstract Pet rabbits have increased their popularity in a lot of countries. However, most of the laboratory profiles in rabbit medicine come from the observations made in rabbit as biomodels or meat production. So that further researches are necessary to obtain reference values for hematology and biochemical profiles in pet rabbits and the different breeds, especially, in relation to acid-base balance. The aim of this report was to offer the mean values of the main parameters connected with acid-base profile in Netherland Dwarf breed. Thirty-five healthy rabbits (15 males and 20 females) were studied. Venous blood sample from lateral saphenous vein was analyzed to measure: haematocrit, haemoglobin, blood urea nitrogen, glucose, blood pH, partial pressure of CO2 (pCO2), total CO2, ions bicarbonate, chloride, sodium, potassium, base excess and anion Gap. Results showed a shorter range that those reported by different researchers. Moreover, differences between genders were showed in pCO2, its values were higher in males. It may be associated with a greater cellular metabolism. Values obtained in this research should be taken into account by veterinary clinicians for this breed in their clinical assessments. Besides, these values provide new results in parameters with few reference values.

Resumo A popularidade de coelhos como animais de estimação aumentou em muitos países. No entanto, a maioria dos perfis de laboratório em medicina de coelhos advém das observações de biomodelos animais ou da produção de carne. Assim, são necessárias pesquisas adicionais para obter valores de referência para hematologia e perfis bioquímicos em coelhos de estimação, e das diferentes raças, especialmente, em relação ao equilíbrio ácido-base. O objetivo deste relatório foi oferecer os valores médios dos principais parâmetros ligados ao perfil ácido-base na raça Anã Holandês. Trinta e cinco coelhos saudáveis (15 machos e 20 fêmeas) foram estudados. A amostra de sangue venoso da veia safena lateral foi analisada para mensuração: hematócrito, hemoglobina, nitrogênio ureico sanguíneo, glicose, pH sanguíneo, pressão parcial de CO2 (pCO2), CO2 total, íons bicarbonato, cloreto, sódio, potássio, excesso de base e ânion Gap. Os resultados apresentaram um intervalo menor do que aqueles relatados por diferentes pesquisadores. Além disso, as diferenças entre os gêneros foram mostradas na pCO2, seus valores foram maiores no sexo masculino. Pode estar associado a um maior metabolismo celular. Os valores obtidos nesta pesquisa devem ser levados em consideração pelos clínicos veterinários para esta raça em suas avaliações clínicas. Além disso, esses valores fornecem novos resultados em parâmetros com poucos valores de referencia.

Termination of lambs fed concentrate or pasture: performance and technical feasibility / Terminação de cordeiros alimentados com concentrado ou pastagem: desempenho e viabilidade técnica

A total of 28 uncastrated lambs, with 20kg of body mass (BM) were distributed into two different finishing systems where animals were fed either concentrate (CFS) or pasture (PFS) until 35 kg BM. The CFS diet consisted of 77.4% of corn grain; 20.2% soybean meal; 1.4% limestone and 1.0% sodium bicarbonate. The PFS diet consisted of both native pasture and ryegrass. The evaluated parameters were: dry matter intake (DMI), organic matter intake (OMI), crude protein intake (CPI), neutral detergent fiber intake (NDFI), feed conversion (FC), mean daily gain, days to reach 35kg BM, ruminal pH, in vivo biometric measurements, carcass composition, percentage of commercial cuts, pallet composition, and proportion of body components. The CFS animals had higher DMI, OMI, CPI and lower NDFI when compared to PFS. Also, animals feed CFS presented higher mean daily gain and better FC. Animals from CFS took 77 days to reach 35kg BM whereas animals from PFS took 278 days. The ruminal pH was higher, and more adequate, in the PFS group when compared to the CFS group. The CFS lambs presented better quantitative characteristics and conformation, and higher fat deposition. PFS lambs, on the other hand, provided leaner and more muscular carcasses. Thus, the termination of lambs without use of bulky feed is technically feasible and provides better zootechnical performance and carcass characteristics. However, the ruminal pH drop may compromise the system and must be observed.(AU)

Conduziu-se ensaio biológico com 28 cordeiros não castrados, com massa corporal (MC) inicial de 20 kg, distribuídos em dois diferentes sistemas alimentares de terminação: concentrado (STC) ou pastagem (STP). A dieta do STC foi constituída de 77,4% de grãos de milho; 20,2% de farelo de soja; 1,4% de calcário e 1,0% de bicarbonato de sódio. A dieta do STP foi constituída de pastagem nativa e azevém. Os animais permaneceram nos sistemas até atingirem 35 kg de MC. Os parâmetros avaliados foram: consumo de matéria seca (CMS), consumo de matéria orgânica (CMO), consumo de proteína bruta (CPB), consumo de fibra em detergente neutro (CFDN), conversão alimentar (CA), ganho médio diário, dias para atingir 35kg, pH ruminal, medidas biométricas in vivo, composição de carcaça, porcentagem de cortes comerciais, composição da paleta e proporção de componentes corporais. Os animais do SFC apresentaram maior CMS, CMO, CPB e menor CFDN quando comparados com o STP. Ainda os animais do STC apresentaram maior ganho médio diário e melhor CA. Os animais do STC levaram 77 dias para atingir 35kg de MC, enquanto os animais do STP levaram 278 dias. O pH ruminal foi maior e mais adequado no STP que no STP. Os cordeiros do STC apresentaram melhores características quantitativas e conformação, e maior deposição de gordura. Por outro lado, o STP forneceu carcaças mais magras e mais musculosas. Assim, a terminação de cordeiros sem utilização de ração volumosa é tecnicamente viável e proporciona melhor desempenho zootécnico e características de carcaça. Entretanto, a queda do pH ruminal pode comprometer o sistema e deve ser observada.(AU)

Effect of Sodium Chloride Replacement with Potassium Chloride on Quality Traits of Bicarbonate-Marinated Turkey Breast Meat

This study aims to evaluate the possibility to formulate low-sodium bicarbonate-marinated turkey breast meat. In total, 60 meat cuts (100 × 50 × 30mm) were divided into four treatments: B1 (0.5% sodium bicarbonate and 0% sodium chloride replacement), B2 (0.5% sodium bicarbonate and 15% sodium chloride replacement), B3 (0.5% sodium bicarbonate and 30% sodium chloride replacement), and B4 (0.5% sodium bicarbonate and 45% sodium chloride replacement). The results showed that sodium replacement up to 45% had no impact on texture (as represented by Allo-Kramer shear values) and water activity. After cooking, Group B1 exhibited the highest L* value (72.1) and the lowest b* (6.6) when compared to other groups. In conclusion, replacing sodium chloride with potassium chloride up to 45% in the presence of sodium bicarbonate did not affect negatively several quality traits (sensory traits, composition, and texture, etc.).(AU)

Effect of Sodium Chloride Replacement with Potassium Chloride on Quality Traits of Bicarbonate-Marinated Turkey Breast Meat

This study aims to evaluate the possibility to formulate low-sodium bicarbonate-marinated turkey breast meat. In total, 60 meat cuts (100 × 50 × 30mm) were divided into four treatments: B1 (0.5% sodium bicarbonate and 0% sodium chloride replacement), B2 (0.5% sodium bicarbonate and 15% sodium chloride replacement), B3 (0.5% sodium bicarbonate and 30% sodium chloride replacement), and B4 (0.5% sodium bicarbonate and 45% sodium chloride replacement). The results showed that sodium replacement up to 45% had no impact on texture (as represented by Allo-Kramer shear values) and water activity. After cooking, Group B1 exhibited the highest L* value (72.1) and the lowest b* (6.6) when compared to other groups. In conclusion, replacing sodium chloride with potassium chloride up to 45% in the presence of sodium bicarbonate did not affect negatively several quality traits (sensory traits, composition, and texture, etc.).

Effect of Dietary Inclusion of Sodium Bicarbonate on Digestibility of Nutrients and Immune Response in Caged Layers During the Summer

The aim of this project was to investigate the effect of dietary inclusion of sodium bicarbonate (NaHCO3) on nutrient digestibility and immune response of caged layers during summer when the temperature exceeds 40 C. For immune response trial, White Leghorn layers (n=160; 24 weeks old) were purchased from a poultry farm and were divided into five treatment/diets groups (4 replicate/treatment). Diet A, was without NaHCO3 and served as control whereas, diets B, C, D, and E contained 0.5, 1.0, 1.5 and 2.0% NaHCO3, respectively. All these birds were vaccinated against Newcastle disease (ND) virus at the start of the experiment and thereafter with one-month intervals. Blood samples were collected from two birds/replicate at 10 days post vaccination each time to check antibody titer against ND virus. For digestibility trial, fecal samples were collected (6 layers/treatment group) at the start of the 37th week of age for two days, at 3 hours interval. Results revealed that immune response against ND virus 10 days post vaccination after 1st, 2nd and 3rd month was significantly (p 0.05) higher in layer birds fed diets containing NaHCO3. Digestibility of dry matter (DM), crude protein (CP), crude fiber (CF), ether extract (EE) and absorption of minerals were also found to be significantly (p 0.05) higher in groups treated with NaHCO3 and the birds fed diet containing 1% NaHCO3 showed the best results. In general, results revealed that 1% supplementation of NaHCO3 in layers diet have a beneficial impact in terms of immunity and diet digestibility.(AU)

Effect of Dietary Inclusion of Sodium Bicarbonate on Digestibility of Nutrients and Immune Response in Caged Layers During the Summer

The aim of this project was to investigate the effect of dietary inclusion of sodium bicarbonate (NaHCO3) on nutrient digestibility and immune response of caged layers during summer when the temperature exceeds 40 C. For immune response trial, White Leghorn layers (n=160; 24 weeks old) were purchased from a poultry farm and were divided into five treatment/diets groups (4 replicate/treatment). Diet A, was without NaHCO3 and served as control whereas, diets B, C, D, and E contained 0.5, 1.0, 1.5 and 2.0% NaHCO3, respectively. All these birds were vaccinated against Newcastle disease (ND) virus at the start of the experiment and thereafter with one-month intervals. Blood samples were collected from two birds/replicate at 10 days post vaccination each time to check antibody titer against ND virus. For digestibility trial, fecal samples were collected (6 layers/treatment group) at the start of the 37th week of age for two days, at 3 hours interval. Results revealed that immune response against ND virus 10 days post vaccination after 1st, 2nd and 3rd month was significantly (p 0.05) higher in layer birds fed diets containing NaHCO3. Digestibility of dry matter (DM), crude protein (CP), crude fiber (CF), ether extract (EE) and absorption of minerals were also found to be significantly (p 0.05) higher in groups treated with NaHCO3 and the birds fed diet containing 1% NaHCO3 showed the best results. In general, results revealed that 1% supplementation of NaHCO3 in layers diet have a beneficial impact in terms of immunity and diet digestibility.

Effects of exercise on cations/anions in blood serum of English Thoroughbred horses / Efeito do exercício sobre cátions/ânions em soro sanguíneo de equinos da raça Puro-Sangue-Inglês

English Thoroughbred horses, are widespread in Mexico and due to the lack of data on their exercise physiology, it is important to conduct exercise tests in order to obtain information the effects of exercise on more essential cations/anions in blood serum, as these horses are submitted to constant efforts. The study was carried out with 150 blood samples of English Thoroughbred horses clinically healthy. The blood sample collection was performed during three periods: 1) rest, 2) 30min after exercise (speed race of 12km/h for 30min with no rest) and 3) 60min after exercise. Mean values were calculated for cations (sodium and potassium) and anions (chloride and bicarbonate). The resulting data set was analyzed using Gaussian distribution and descriptive statistics. Confidence intervals of 95% were established. The linear relationships between ions were quantified, and an analysis of variance was performed to compare the mean values between groups. The concentrations of the described analytes are consistent with values reported by international literature. The comparison between groups, revealed that during exercise, sodium ion did not show changes 30min after exercise and increase 60min after. Potassium ion showed increase 30min after exercise and decrease 60min after. Chloride ion showed a decrease 30min after exercise, to recover gradually 60min after. Meanwhile, bicarbonate ion showed increase 30min after exercise, decreasing slightly in the final stage. Negative correlation between bicarbonate ion and chloride ion were determined. It was concluded that exercise tests are useful for the determination of acid-base balance and osmotic balance, and their main role is to evaluate the athletic ability of horses.Considering that chloride ion excretion and metabolic adjustments of potassium ion and bicarbonate ion are superior to water loss, compared to the normal osmolarity of blood serum. The results found can be used to structure an adequate replacement program of electrolytes lost in sweat.(AU)

Equinos da raça Puro-Sangue-Inglês são difundidos no México e, devido à falta de dados sobre sua fisiologia do exercício, é importante fazer testes de exercício para obter informações sobre os efeitos do exercício em cátions/ânions mais essenciais no soro do sangue, pois esses equinos são submetidos a esforços constantes. O estudo foi realizado com 150 amostras de sangue de equinos Puro-Sangue-Inglês, clinicamente saudáveis. A coleta de sangue foi realizada em três períodos: 1) descanso, 2) 30min após o exercício (corrida de velocidade de 12km/h por 30min, sem descanso) e 3) 60min após o exercício. Os valores médios foram calculados para cátions (sódio e potássio) e ânions (cloreto e bicarbonato). O conjunto de dados resultante foi analisado utilizando-se distribuição gaussiana e estatística descritiva. Intervalos de confiança de 95% foram estabelecidos. As relações lineares entre os íons foram quantificadas, e uma análise de variância foi realizada para se compararem os valores médios entre grupos. As concentrações dos analitos descritos são consistentes com os valores relatados na literatura internacional. A comparação entre os grupos revelou que, durante o exercício, o sódio íon não mostrou alterações 30min após o exercício e aumentou 60min após. O potássio íon mostrou aumento 30min após o exercício e diminuiu 60min após. O cloreto íon mostrou uma diminuição 30min após o exercício, para recuperar gradualmente 60min depois. O bicarbonato íon mostrou aumento 30min após o exercício, diminuindo ligeiramente no estágio final. Correlação negativa entre bicarbonato íon e cloreto íon foi encontrada. Concluiu-se que os testes de exercício são úteis para a determinação do equilíbrio ácido-base e do equilíbrio osmótico, e seu principal papel é avaliar a capacidade atlética dos equinos. Considerando-se que a excreção de cloro íon e ajustes metabólicos de potássio íon e bicarbonato íon são superiores à perda de água, comparada à osmolaridade normal do soro sanguíneo,os resultados encontrados podem ser usados para estruturar um programa adequado de reposição de eletrólitos perdidos no suor.(AU)

Effects of exercise on cations/anions in blood serum of English Thoroughbred horses / Efeito do exercício sobre cátions/ânions em soro sanguíneo de equinos da raça Puro-Sangue-Inglês

English Thoroughbred horses, are widespread in Mexico and due to the lack of data on their exercise physiology, it is important to conduct exercise tests in order to obtain information the effects of exercise on more essential cations/anions in blood serum, as these horses are submitted to constant efforts. The study was carried out with 150 blood samples of English Thoroughbred horses clinically healthy. The blood sample collection was performed during three periods: 1) rest, 2) 30min after exercise (speed race of 12km/h for 30min with no rest) and 3) 60min after exercise. Mean values were calculated for cations (sodium and potassium) and anions (chloride and bicarbonate). The resulting data set was analyzed using Gaussian distribution and descriptive statistics. Confidence intervals of 95% were established. The linear relationships between ions were quantified, and an analysis of variance was performed to compare the mean values between groups. The concentrations of the described analytes are consistent with values reported by international literature. The comparison between groups, revealed that during exercise, sodium ion did not show changes 30min after exercise and increase 60min after. Potassium ion showed increase 30min after exercise and decrease 60min after. Chloride ion showed a decrease 30min after exercise, to recover gradually 60min after. Meanwhile, bicarbonate ion showed increase 30min after exercise, decreasing slightly in the final stage. Negative correlation between bicarbonate ion and chloride ion were determined. It was concluded that exercise tests are useful for the determination of acid-base balance and osmotic balance, and their main role is to evaluate the athletic ability of horses.Considering that chloride ion excretion and metabolic adjustments of potassium ion and bicarbonate ion are superior to water loss, compared to the normal osmolarity of blood serum. The results found can be used to structure an adequate replacement program of electrolytes lost in sweat.(AU)

Equinos da raça Puro-Sangue-Inglês são difundidos no México e, devido à falta de dados sobre sua fisiologia do exercício, é importante fazer testes de exercício para obter informações sobre os efeitos do exercício em cátions/ânions mais essenciais no soro do sangue, pois esses equinos são submetidos a esforços constantes. O estudo foi realizado com 150 amostras de sangue de equinos Puro-Sangue-Inglês, clinicamente saudáveis. A coleta de sangue foi realizada em três períodos: 1) descanso, 2) 30min após o exercício (corrida de velocidade de 12km/h por 30min, sem descanso) e 3) 60min após o exercício. Os valores médios foram calculados para cátions (sódio e potássio) e ânions (cloreto e bicarbonato). O conjunto de dados resultante foi analisado utilizando-se distribuição gaussiana e estatística descritiva. Intervalos de confiança de 95% foram estabelecidos. As relações lineares entre os íons foram quantificadas, e uma análise de variância foi realizada para se compararem os valores médios entre grupos. As concentrações dos analitos descritos são consistentes com os valores relatados na literatura internacional. A comparação entre os grupos revelou que, durante o exercício, o sódio íon não mostrou alterações 30min após o exercício e aumentou 60min após. O potássio íon mostrou aumento 30min após o exercício e diminuiu 60min após. O cloreto íon mostrou uma diminuição 30min após o exercício, para recuperar gradualmente 60min depois. O bicarbonato íon mostrou aumento 30min após o exercício, diminuindo ligeiramente no estágio final. Correlação negativa entre bicarbonato íon e cloreto íon foi encontrada. Concluiu-se que os testes de exercício são úteis para a determinação do equilíbrio ácido-base e do equilíbrio osmótico, e seu principal papel é avaliar a capacidade atlética dos equinos. Considerando-se que a excreção de cloro íon e ajustes metabólicos de potássio íon e bicarbonato íon são superiores à perda de água, comparada à osmolaridade normal do soro sanguíneo,os resultados encontrados podem ser usados para estruturar um programa adequado de reposição de eletrólitos perdidos no suor.(AU)

Comparison of electrolyte and acid-base balances of Dorper breed ewes between single and twin pregnancies

ABSTRACT: During pregnancy there are modifications in the metabolic profile of sheep that may predispose to the occurrence of metabolic disorders, of which pregnancy toxaemia (PT) is highlighted. Blood gas analysis is detects changes in acid-base and electrolyte balance effectively. The objectives of this study were to study the acid-base and electrolyte balance of sheep during gestation and in the immediate peripartum (up to 48 hours postpartum), comparing single gestation with twins. Sixty healthy sheep of Dorper breed, two to five years old were raised in a semi-intensive system and were divided in two experimental groups: Group 1: 30 ewes, with ultrasonographic diagnosis of single fetus gestation; Group 2: 30 ewes, with ultrasonographic diagnosis of twin pregnancy. The experimental moments were defined as: MI-immediately after artificial insemination (control); MG30 - 30 days of gestation; MG90 - 90 days of gestation; MG120 - 120 days of gestation; MG130 - 130 days of gestation; MG140 - 140 days of gestation; MP - lambing; MPP1 - 24h postpartum; MPP2 - 48h postpartum. At all times 1mL of blood was collected per jugular vein puncture for blood gas evaluation in a portable equipament (I-Stat®). The pH, carbon dioxide pressure (PCO2), bicarbonate (HCO3-), base excess (BE), sodium (Na+), potassium (K+), chloride (Cl-), ionized calcium (Ca+2), lactate, anion gap (AG) were mensured and strong ion difference (SID) were calculed. This work showed that there were changes in acid-base and electrolyte balance in pregnant ewes, due to the decrease in BE, HCO3-, TCO2 and increase of lactate and AG during gestation, but the pH remained normal and did not present any difference among moments in both groups. Comparing the groups, single-gestation ewes presented higher alkaline expenditure at delivery than twin-gestation, evidenced by lower levels of BE and HCO3-. Lower Na+ levels were observed in prepartum; drop in K+ values with advancing gestation; hyperchloremia and hypocalcemia during gestation according to the reference standards for species; decreased iCa+2 levels in the final third of gestation in both groups. It was concluded that the ewes of this study were healthy until the last moment analyzed (48h postpartum), although have shown greater consumption of the alkaline reserve in the peripartum, being more explicit in the single gestation animals. In addition, this situation can be physiological and result in increased energy demand during gestation, more exacerbated by peripartum.

RESUMO: Durante a gestação ocorrem modificações metabólicas nas ovelhas que podem predispor a ocorrência de transtornos metabólicos, dos quais se destaca a toxemia da prenhez (TP). A hemogasometria é um exame que detecta alterações nos equilíbrios ácido-básico e eletrolítico de forma eficaz. Os objetivos deste estudo foram estudar os equilíbrios ácido-básico e eletrolítico de ovelhas durante a gestação e no periparto imediato (até 48 horas pós-parto), comparando-se gestação única com gemelar. Foram utilizadas 60 ovelhas criadas em manejo semi-intensivo, hígidas, da raça Dorper, com dois a cinco anos de idade. Foram constituídos dois grupos experimentais: Grupo 1: 30 ovelhas, com diagnóstico ultrassonográfico de gestação de feto único; Grupo 2: 30 ovelhas, com diagnóstico ultrassonográfico de gestação gemelar. Os momentos experimentais foram definidos como: MI - imediatamente após a inseminação artificial (controle); MG30 - 30 dias de gestação; MG90 - 90 dias de gestação; MG120 - 120 dias de gestação; MG130 - 130 dias de gestação; MG140 - 140 dias de gestação; MP - dia do parto; MPP1 - 24h pós-parto; MPP2 - 48h pós-parto. Em todos os momentos foi colhido 1mL de sangue por punção da veia jugular para avaliação hemogasométrica em aparelho portátil (I-Stat®). Foram analisados os parâmetros: pH, pressão de dióxido de carbono (PCO2), bicarbonato (HCO3-), excesso de bases (EB), sódio (Na+), potássio (K+), cloreto (Cl-), cálcio ionizado (iCa+2), lactato, ânion gap (AG) e diferença de íons fortes (SID). Este trabalho mostrou que houve mudanças nos equilíbrios ácido-básico e eletrolítico nas ovelhas prenhes, pela diminuição do EB, HCO3- e aumento do lactato e AG no decorrer da gestação, porém o pH se manteve dentro da normalidade e não apresentou diferença ao longo dos momentos em ambos os grupos. Comparando os grupos, as ovelhas de gestação única apresentaram maior consumo da reserva alcalina no momento do parto do que as de gestação gemelar, evidenciado menores níveis de EB e HCO3-. Foram observados níveis mais baixos de Na+ no pré-parto; queda dos valores de K+ com o avanço da gestação; hipercloremia e hipocalcemia durante a gestação segundo os padrões de referência para espécie e diminuição dos níveis de iCa+2 no terço final da gestação nas ovelhas de ambos os grupos. Concluiu-se que as ovelhas deste estudo apresentaram-se saudáveis até o último momento analisado (48h pós-parto), embora tenham mostrado maior consumo da reserva alcalina no periparto, sendo mais evidente nos animais de gestação única. Apesar disso, essa ocorrência pode ser considerada fisiológica e consequência do aumento da demanda energética durante a gestação, mais exacerbada no periparto.

Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep / Protocolo de indução experimental de acidose metabólica por administração intravenosa de ácido clorídrico em ovinos

The aim of this study was to assess the magnitude and duration of blood and urine changes and the side effects of hyperchloremic acidosis induced by the intravenous administration of hydrochloric acid in sheep. Five healthy, crossbred adult ewes, with a mean body weight of 44±2.9kg were used. The hydrochloric acid solution was administered intravenously at a rate of 25mL/kg/h for 4 hours continuously. Venous blood and urine samples were collected and pH values, blood carbon dioxide partial pressure, bicarbonate, base excess, strong ion difference, anion gap, total concentration of nonvolatile buffers, creatinine, plasma L-lactate, plasma and urine sodium, potassium, and chloride were determined. The experimental protocol induced severe hyperchloremic acidosis at the end of the infusion, with a decreased plasma strong ion difference. The fractional excretion of sodium and chloride remained increased during 4 hours after the infusion. Aciduria was observed at approximately 24 hours. Twenty-four hours after the infusion, the animals showed mild and compensated metabolic acidosis. This protocol was effective in inducing severe and long-lasting hyperchloremic acidosis and did not cause serious side effects. Therefore, this protocol can be used safely in adult sheep for studies on the treatment of this condition.(AU)

O objetivo deste estudo foi avaliar a magnitude e a duração das alterações sanguíneas e urinárias, bem como os efeitos colaterais da acidose hiperclorêmica induzida por administração intravenosa de ácido clorídrico, em ovinos. Foram utilizadas cinco ovelhas mestiças, adultas, sadias, com peso médio de 44±2,9kg. A solução de ácido clorídrico foi administrada por via intravenosa, na velocidade de 25mL/kg/h, totalizando quatro horas de administração contínua. Amostras de sangue venoso e de urina foram colhidas, e determinaram-se os valores de pH, pressão parcial de dióxido de carbono, bicarbonato, excesso de bases, diferença dos íons fortes, ânion-gap, creatinina, lactato L, sódio, potássio e cloro. O protocolo de indução experimental foi capaz de induzir acidose hiperclorêmica grave ao término da infusão, com diminuição da diferença dos íons fortes plasmáticos. Houve aumento da excreção fracionada de sódio e cloro por até quatro horas após o término da infusão. A acidúria foi observada por cerca de 24 horas. Após 24 horas do início da infusão, os animais apresentaram acidose metabólica leve e compensada. Esse protocolo foi eficaz na indução da acidose hiperclorêmica grave e duradoura e não causou efeitos colaterais. Conclui-se que o protocolo pode ser usado com segurança em ovelhas adultas, para estudos sobre tratamento dessa condição.(AU)