Pyometra in a tiger (Panthera tigris)

Background: Captive tigers can live a long life, around 26 years. Among the diseases described some of non-infectious origin are quite common, such as chronic kidney disease, spondylosis, and biliary cysts or tumors. On the other hand, pyometra has been frequently reported in lions, who have a higher risk of developing the disease than tigers and leopards. Pyometra is a disease with few descriptions in tigers and it may be related to the physiological features of the species. The animal is listed as Endangered on the International Union for the Conservation of Nature Red List of Threatened. The present report aims to describe the diagnosis and treatment of pyometra in a captive tigress. Case: A 7-year-old entire female tiger (Panthera tigris) weighing 140 kg was presented with a 3-day history of anorexia and prostration. For clinical examinations, collection of laboratory and imaging tests, the patient initially underwent dissociative anesthesia to allow catheterization of the cephalic vein and intravenous general anesthesia for orotracheal intubation followed by anesthetic maintenance in isoflurane. On general physical examination, the animal had normal colored mucosa, vital parameters within normal limits, and a body condition score of 6 on a scale of 9. There was no presence of vulvar secretion. The blood count and the biochemical exams showed values within the normal range for the species. The chest X-ray in the right and left views did not demonstrate pulmonary abnormalities. Ultrasonographic examination of the abdomen showed distension of the uterine body and horns, which have intraluminal hyperechoic fluid content without flocculation. Based on the imaging exam, the diagnosis was suggestive of pyometra. Exploratory celiotomy was performed via ventral midline, confirming the condition, which was treated by ovariohysterectomy. The surgical technique was performed as described for therapeutic ovariohysterectomy in dogs and cats. Culture of uterine content identified Escherichia coli. The histological analysis identified diffuse endometritis associated with follicular cysts. The tiger had complete recovery without any complications. The patient was releasing 13 days after the surgical procedure and in the last contact four months after the surgery, it was in perfect health conditions. Discussion: Pyometra in large exotic felids has been occasionally reported, mainly in animals more than 10 years of age. Although the tigress in the report is estimated to be seven years old. The patient in question started with anorexia and prostration and as there was already a history of cystic endometrial hyperplasia, a possible pyometra was suspected, despite being uncommon in the species. There was not vaginal discharge. The definitive diagnosis was by means of ultrasound examination and ovariohysterectomy was performed. Abdominal surgery for these large felids is complex, due to the intra-abdominal volume the flank approach or by laparoscopic is suggested, however in this case a ventral midline incision was performed without intercurrences and complications in the post-operative period. The surgical technique like that used in small animals was effective for the treatment of pyometra in the tigress with the use of ovariohysterectomy. Culture of uterine content identified Escherichia coli, which has been the most commonly isolated pathogen in pyometra of large felids. It was concluded that, as in bitches with pyometra, early diagnosis and surgical treatment is ideal for the patient's recovery.

Using vaginal discharge score (VDS) grading system to evaluate the effect of clinical endometritis on reproductive performance of dairy cows in China

Abstract Clinical endometritis (CE) is a major cause in affecting the reproductive performance of dairy cows. The objectives of this study were to ascertain the prevalence of CE and to evaluate the effect of CE on reproductive performance in dairy cows using vaginal discharge score (VDS) grading system. 803 dairy cows were examined by vaginoscope with 4-point VDS at 26 ± 3 days in milk (DIM) and classified into six groups: non-endometritis with VDS 0 (control; CON), endometritis with VDS 1 (MEM), non-treated endometritis with VDS 2 (NTME), treated endometritis with VDS 2 (TME), non-treated endometritis with VDS 3 (NTPE), and treated endometritis with VDS 3 (TPE). Cows in TME and TPE groups were treated with 200 mL of 50% dextrose solution by intrauterine infusion. The prevalence of CE was 33% at 26 ± 3 DIM. Binary logistic regression analysis revealed cows in MEM, NTME and NTPE groups had a less likelihood of first artificial insemination (AI) pregnancy than those in CON group (P < 0.05). Kaplan-Meier survival curves for days open were statistically different (P = 0.004). In Cox regression model, cows in NTME and NTPE groups had a reduced pregnancy rate than those in CON group (P < 0.05). The hazard of pregnancy in NTME group was lower than that in TME group (P = 0.044). Similarly, it was lower for the hazard of pregnancy in NTPE group than in TPE group (P = 0.048). Cows in MEM, NTME, and NTPE groups required more services per pregnancy than those in CON group (P < 0.05). In conclusion, CE examined by the VDS grading system impaired reproductive performance, and mild endometritis with VDS 1 should be treated in the early postpartum period to ameliorate fertility in dairy herds.

Using vaginal discharge score (VDS) grading system to evaluate the effect of clinical endometritis on reproductive performance of dairy cows in China

Clinical endometritis (CE) is a major cause in affecting the reproductive performance of dairy cows. The objectives of this study were to ascertain the prevalence of CE and to evaluate the effect of CE on reproductive performance in dairy cows using vaginal discharge score (VDS) grading system. 803 dairy cows were examined by vaginoscope with 4-point VDS at 26 ± 3 days in milk (DIM) and classified into six groups: non-endometritis with VDS 0 (control; CON), endometritis with VDS 1 (MEM), non-treated endometritis with VDS 2 (NTME), treated endometritis with VDS 2 (TME), non-treated endometritis with VDS 3 (NTPE), and treated endometritis with VDS 3 (TPE). Cows in TME and TPE groups were treated with 200 mL of 50% dextrose solution by intrauterine infusion. The prevalence of CE was 33% at 26 ± 3 DIM. Binary logistic regression analysis revealed cows in MEM, NTME and NTPE groups had a less likelihood of first artificial insemination (AI) pregnancy than those in CON group (P 0.05). Kaplan-Meier survival curves for days open were statistically different (P = 0.004). In Cox regression model, cows in NTME and NTPE groups had a reduced pregnancy rate than those in CON group (P 0.05). The hazard of pregnancy in NTME group was lower than that in TME group (P = 0.044). Similarly, it was lower for the hazard of pregnancy in NTPE group than in TPE group (P = 0.048). Cows in MEM, NTME, and NTPE groups required more services per pregnancy than those in CON group (P 0.05). In conclusion, CE examined by the VDS grading system impaired reproductive performance, and mild endometritis with VDS 1 should be treated in the early postpartum period to ameliorate fertility in dairy herds.(AU)

Endometritis in dairy cows reared in tropical conditions: microorganisms, reproductive performance and natural alternative therapy

Doenças uterinas causam redução da lucratividade das granjas leiteiras além de prejudicar a fertilidade das vacas. No entanto, faltam informações detalhadas sobre a eficiência reprodutiva e produtiva de vacas leiteiras afetadas por doenças uterinas em condições tropicais, além da identificação dos fatores e dos microrganismos associados com a incidência dessa enfermidade. Destaca-se, também, a importância de estudos relacionados aos possíveis tratamentos naturais e não convencionais para doenças uterinas em substituição à antibioticoterapia. Dessa forma, essa tese foi estruturada em três estudos: 1) Identificar os principais microrganismos presentes no ambiente uterino de vacas leiteiras com doenças uterinas durante o puerpério; 2) Identificar os principais fatores associados à incidência de doenças uterinas e avaliar o seu impacto no desempenho reprodutivo e na produção de leite. 3) Avaliar a ação antibacteriana in vitro pelo teste de difusão em disco de sete óleos essenciais (alecrim, canela, cravo, eucalipto, limão, orégano e tomilho) contra as cepas padrão de Escherichia coli (ATCC 25922), Fusobacterium necrophorum (ATCC 25286) e Trueperella pyogenes (ATCC 19411). No estudo 1, o objetivo foi avaliar os principais patógenos relacionados aos casos de endometrite clínica (EC) e endometrite subclínica (ES) em amostras uterinas de vacas leiteiras em rebanhos brasileiros. Foram utilizados meios seletivos e diferenciais para o isolamento de bactérias aeróbicas e anaeróbicas e posterior identificação por espectrometria de massa (MALDI-TOF MS). Um total de 279 vacas Holandesas em lactação com 28 a 33 dias em leite de seis fazendas comerciais foi avaliado. Inicialmente, as vacas foram classificadas em três grupos: vacas saudáveis (n = 161), vacas com EC (n = 83) e vacas com ES (n = 35). Um total de 127 espécies bacterianas de 48 gêneros foi identificado. Animais saudáveis apresentaram 97 espécies, seguidos do grupo EC com 53 espécies, enquanto vacas ES apresentaram apenas 21 espécies bacterianas. Houve uma taxa de isolamento significativamente maior de Trueperella pyogenes em vacas com EC (26,5%) em comparação com vacas saudáveis e ES. Algumas espécies anaeróbias foram isoladas exclusivamente do grupo EC. Curiosamente, 18,1% das amostras de vacas EC e 40% das vacas ES foram negativas ao isolamento bacteriano. No estudo 2, o objetivo foi investigar os impactos da endometrite clínica e da endometrite subclínica no desempenho reprodutivo e na produção de leite de vacas criadas em condições tropicais. Um total de 279 vacas Holandesas em lactação (28 a 33 dias em leite) de seis fazendas comerciais foram estudadas. Os animais foram classificados em três grupos: vacas saudáveis (sem EC e ES, n = 161), vacas com EC (escore de corrimento vaginal = 3 e 18% PMNL, n = 83) e vacas com ES (ausência de sinais de EC e > 18% PMNL, n = 35). Vacas com EC apresentaram menor taxa de concepção à primeira IA (P < 0,05), e ambas as vacas com EC e ES necessitaram maior número de serviços e de dias para emprenhar (P < 0,05), além de produzirem menos leite do que vacas saudáveis (P < 0,05). Nenhum fator avaliado neste estudo foi associado à incidência de EC e ES (P > 0,05). Em conclusão, foi evidenciado impacto negativo de EC e ES no desempenho reprodutivo e na produção de leite de vacas leiteiras. No estudo 3, foi investigada a atividade antibacteriana de sete óleos essenciais contra as cepas de referência de Escherichia coli, Fusobacterium necrophorum, Trueperella pyogenes e Staphylococcus aureus. O ensaio de difusão em disco revelou que os óleos essenciais de canela, cravo, orégano e tomilho apresentaram maior zona de inibição contra todas as bactérias avaliadas. Esses achados indicam que os óleos essenciais apresentam potencial para serem utilizados como alternativa no tratamento da endometrite bovina. No geral, os achados da presente tese revelaram quais são os principais microrganismos presentes no ambiente uterino de vacas leiterias com e sem endometrite criadas em condições tropicais. Verificou-se, ainda, que a endometrite clínica e subclínica prejudicaram a performance reprodutiva e a produção de leite. Por fim, os óleos essenciais apresentam forte atividade antibacteriana diante dos principais patógenos associados com doenças uterinas em vacas leiteiras.

Uterine diseases reduce the profitability of dairy farms in addition to impairing the fertility of dairy cows. However, there is a lack of detailed information on the reproductive and productive performance of dairy cows affected by uterine diseases in tropical conditions, in addition to identification of factors and microorganisms associated with the incidence of this disease. The importance of studies related to possible natural and unconventional treatments for uterine in replacement of antibiotic therapy is also highlighted. Thus, this thesis was structured in three studies: 1) Identify the main microorganisms present in the uterine environment of dairy cows with uterine diseases during the puerperium; 2) Identify the main factors associated with the incidence of uterine diseases in dairy cows and assess the impact their impact on reproductive performance and milk production. 3) Evaluate the in vitro antibacterial action by disk diffusion assay of seven essential oils (rosemary, cinnamon, clove, eucalyptus, lemon, oregano and thyme) against the reference strains of Escherichia coli (ATCC 25922), Fusobacterium necrophorum (ATCC 25286) and Trueperella pyogenes (ATCC 19411). In study 1, the objective was to assess the main pathogens related to cases of clinical endometritis (CE) and subclinical endometritis (SE) from uterine samples of dairy cows in Brazilian herds. Selective and differential media were used for isolation of aerobic and anaerobic bacteria and subsequent identification by mass spectrometry (MALDI-TOF MS). A total of 279 lactating Holstein cows with 28 to 33 days in milk from six commercial farms were evaluated. Initially, cows were classified in three groups: cytologic healthy cows (n = 161), cows with CE (n = 83), and cows with SE (n = 35). A total of 127 bacterial species of 48 genera were identified. Healthy animals presented 97 species, followed by the CE group with 53 identified species, while SE cows had only 21 bacterial species. There was a significantly higher isolation rate of Trueperella pyogenes in CE cows (26.5%) compared to healthy and SE cows. Some anaerobic species were exclusively isolated from the CE group. Interestingly, 18.1% of samples from CE cows and 40% of SE cows were negative to bacterial isolation. In study 2, the objective was to investigate the impacts of clinical endometritis and subclinical endometritis on the reproductive performance and milk production of cows reared in tropical conditions. A total of 279 lactating Holstein dairy cows (28 to 33 d in milk) from six commercial farms were studied. The animals were classified into three groups: healthy cows (without CE and SE, n = 161), cows with CE (vaginal discharge score = 3 and 18% PMNL, n = 83) and cows with SE (absence of signs of CE and > 18% PMNL, n = 35). Cows with CE had a lower conception rate at first AI (P < 0.05), and both cows with CE and SE required more services and days to be pregnant (P < 0.05), in addition to producing less milk than healthy cows (P < 0.05). No factor evaluated in this study was associated with the incidence of CE and SE (P < 0.05). In conclusion, a negative impact of CE and SE on the reproductive performance and milk production of dairy cows was evidenced. In study 3, the antibacterial activity of seven essential oils against the reference strains of Escherichia coli, Fusobacterium necrophorum, Trueperella pyogenes and Staphylococcus aureus was investigated. The disk diffusion test revealed that the essential oils of cinnamon, clove, oregano and thyme showed presented a greater zone of inhibition against all bacteria evaluated. These findings indicate that essential oils have potential to be used as an alternative in the treatment of bovine endometritis. Overall, the findings of this thesis reveal the main microorganisms present in the uterine environment of cows with and without endometritis raised in tropical conditions. It was also found that clinical and subclinical endometritis impaired reproductive performance and milk production. Finally, essential oils have a strong antibacterial activity against the main pathogens associated with uterine diseases in dairy cows.

Metritis and endometritis in high yielding dairy cows / Metrite e endometriose em vacas de leite de alto rendimento

Dairy cows have shown a steep increase in milk production, which is unfortunately characterized by a dramatic decline in reproductive performance. In these modern high yielding cows authors worldwide mention a higher number of puerperal problems, such as retained placenta, acute metritis and abnormal vaginal discharge. All of which are known to negatively influence reproductive capacity of these animals. Cows affected by retained placenta and/or acute metritis are furthermore at a significantly higher risk of other typical ‘dairy cow diseases such as acetonaemia, displaced abomasum and cystic ovarian disease. It is therefore important that the management of the cows during the transition period should be optimized in order to prevent cows from these disease complexes. Furthermore, during the immediate postpartum period, cows should be looked after properly in order to identify animals that are affected by clinical uterine disease as soon as possible so that treatment should not be delayed. High numbers of cows are furthermore reported to suffer from clinical and subclinical endometritis, both giving rise to significant problems to get in calf. At the moment, there is a lot of debate concerning both the diagnosis as well as the first choice treatment of cows suffering from clinical and subclinical endometritis. Therefore, also for these diseases prevention is far more effective than treatment.(AU)

Vacas de leite tem mostrado um aumento marcante na produção de leite, que infelizmente é caracterizado por um declínio dramático no desempenho reprodutor. Com relação a estas vacas de leite modernas de alto rendimento autores ao redor do mundo mencionam diversos problemas de puerpério, como placenta retida, metrite aguda e descarga vaginal anormal. Todos estes conhecidamente afetam a capacidade reprodutora destes animais. Vacas afetadas por placenta retida e/ou metrite aguda estão também em maior risco de outros tipos de “doenças de vacas de leite” como acetonaemia, abomaso deslocado e doença de ovário cístico. É importante, portanto, que o gerenciamento de vacas durante o período de transição deve ser otimizado para prevenir vacas contra estas doenças complexas. Além disto, durante o período pós parto imediato deve haver o cuidado apropriado das vacas para identificar animais afetados por doença uterina clínica assim que possível para não atrasar o tratamento. Um alto número de vacas sofrem de endometriose clínica e subclínica, ambos levando a problemas significativos para reproduzir. No momento há muito debate com relação ao diagnóstico e também ao primeiro tratamento de vacas sofrendo de endometriose clínica e subclínica. Portanto, a prevenção destas doenças também é muito mais eficiente do que o tratamento.(AU)

Metritis and endometritis in high yielding dairy cows / Metrite e endometriose em vacas de leite de alto rendimento

Dairy cows have shown a steep increase in milk production, which is unfortunately characterized by a dramatic decline in reproductive performance. In these modern high yielding cows authors worldwide mention a higher number of puerperal problems, such as retained placenta, acute metritis and abnormal vaginal discharge. All of which are known to negatively influence reproductive capacity of these animals. Cows affected by retained placenta and/or acute metritis are furthermore at a significantly higher risk of other typical ‘dairy cow diseases’ such as acetonaemia, displaced abomasum and cystic ovarian disease. It is therefore important that the management of the cows during the transition period should be optimized in order to prevent cows from these disease complexes. Furthermore, during the immediate postpartum period, cows should be looked after properly in order to identify animals that are affected by clinical uterine disease as soon as possible so that treatment should not be delayed. High numbers of cows are furthermore reported to suffer from clinical and subclinical endometritis, both giving rise to significant problems to get in calf. At the moment, there is a lot of debate concerning both the diagnosis as well as the first choice treatment of cows suffering from clinical and subclinical endometritis. Therefore, also for these diseases prevention is far more effective than treatment.

Vacas de leite tem mostrado um aumento marcante na produção de leite, que infelizmente é caracterizado por um declínio dramático no desempenho reprodutor. Com relação a estas vacas de leite modernas de alto rendimento autores ao redor do mundo mencionam diversos problemas de puerpério, como placenta retida, metrite aguda e descarga vaginal anormal. Todos estes conhecidamente afetam a capacidade reprodutora destes animais. Vacas afetadas por placenta retida e/ou metrite aguda estão também em maior risco de outros tipos de “doenças de vacas de leite” como acetonaemia, abomaso deslocado e doença de ovário cístico. É importante, portanto, que o gerenciamento de vacas durante o período de transição deve ser otimizado para prevenir vacas contra estas doenças complexas. Além disto, durante o período pós parto imediato deve haver o cuidado apropriado das vacas para identificar animais afetados por doença uterina clínica assim que possível para não atrasar o tratamento. Um alto número de vacas sofrem de endometriose clínica e subclínica, ambos levando a problemas significativos para reproduzir. No momento há muito debate com relação ao diagnóstico e também ao primeiro tratamento de vacas sofrendo de endometriose clínica e subclínica. Portanto, a prevenção destas doenças também é muito mais eficiente do que o tratamento.

Perfil metabólico energético e enzimático hepático e sua influência no retorno à ciclicidade e ocorrência de infecções uterinas pós-parto em vacas leiteiras cruzadas Gir x Holandês.

Desordens metabólicas e reprodutivas afetam vacas leiteiras no pós-parto apresentando relevante importância econômica, pois reduzem a produção. Objetivou-se verificar a influência das alterações clínicas e reprodutivas, escore de condição corporal (ECC), perfil metabólico energético e enzimático hepático no retorno à ciclicidade ovariana e ocorrência de infecções uterinas pós-parto em fêmeas leiteiras. Foram utilizadas 53 fêmeas cruzadas Gir x Holandês, provenientes de propriedades do Sul do Espírito Santo, subdivididas em dois grupos: Primíparas, com 19 fêmeas de primeira cria e; Multíparas, com 34 fêmeas com mais de dois partos. Avaliou-se os seguintes momentos: 15 dias pré-parto e, 15, 30, 45 e 60 dias pós-parto. No exame físico avaliou-se as frequências cardíaca (FC) e respiratória (FR), temperatura retal (TºR), peso e ECC. Foram coletadas amostras sanguíneas por meio de venopunção da veia coccígea para avaliação das concentrações séricas de -hidroxibutirato, colesterol total e lipoproteínas (perfil energético) e alanina aminotransferase (ALT), gama glutamiltransferase (GGT) e fosfatase alcalina (FA) (perfil enzimático hepático). O exame ginecológico foi realizado por meio de palpação e ultrassonografia transretal. O útero foi avaliado quanto ao tamanho, posição, consistência e simetria de cornos uterinos para acompanhamento do processo de involução uterina e os ovários, quanto ao tamanho e presença de estruturas ovarianas para determinação do retorno à atividade ovariana luteal cíclica. Realizou-se o exame de vaginoscopia para avaliação da presença e características das secreções vaginais. As infecções uterinas foram classificadas como metrite clínica e puerperal e endometrite clínica de acordo com a característica e tempo da secreção liberada e; endometrite subclínica quando diagnosticada por citologia endometrial. As variáveis paramétricas foram submetidas ao teste de Tukey e variáveis não paramétricas, ao teste de Kruskall-Wallis. Para correlação, utilizou-se o teste de correlação de Spearman; todos com nível de significância de 5%. Os parâmetros clínicos de FC, FR e TºR encontraram-se dentro dos limites de normalidade estabelecidos para a espécie. O ECC variou de 4 a 2,5 e o peso, de 273 a 315kg; observou-se diminuição nos dois parâmetros do momento D-15 para os demais, indicando a importância da adoção de estratégias que minimizem os efeitos da perda de condição corporal pós-parto.; sendo observadas diferenças entre os grupos em todos os momentos avaliados para o peso. Aos 60 dias pós-parto 33,96%(18/53) dos animais haviam retomado a atividade ovariana. Não observou-se diferença no tamanho dos ovários entre momentos e grupos. Em relação ao tamanho de útero e cérvix notou-se diminuição entre D-15 e os demais, entretanto, não observou-se diferenças entre grupos. A incidência de infecções uterinas foi de 33,96%(18/53), sendo que 27,78%(5/18) dos animais que cursaram a enfermidade eram primíparas. Dos animais que apresentaram infecção uterina, 50%(9/18) apresentaram metrite clínica, dos quais 33,33% eram primíparas e 50%(9/18) apresentaram endometrite clínica, sendo 22,22% primíparas. Nenhum animal apresentou endometrite subclínica. A perda de ECC pós-parto não influenciou na ocorrência de infecções uterinas e no retorno à ciclicidade. Não houve correlação entre a ocorrência de cetose e infecções uterinas nem com o tempo de retorno ao cio pós-parto. Não foram observadas alterações no perfil metabólico enzimático hepático.

Metabolic and reproductive disorders have an economic impact on livestock production mainly affecting dairy cattle during post-partum reducing production. The aim of this study is verify the clinic and reproductive disorders influence, body condition score, metabolic energy and enzymatic liver function in the return of ovarian cyclicity and the occurrence of uterine infections during post-partum in dairy cattle. In this research was used 53 females crossbreed with Gyr x Holland from South of Espírito Santo. It was divided in two groups, first one primiparous cows with 19 females and the second one pluriparous cows with 34 females giving birth more than twice. These groups were evaluated during 15 days prepartum and during postpartum for 15, 30, 45 and 60 days. In addition, was evaluated as well in the physical exam cardiac and respiratory frequency, rectal temperature, weight and body condition score. Blood samples was collected by means of venipuncture process from coccygeal vein for serum evaluation. The concentrations of betahydroxybutyrate, total cholesterol and lipoproteins, alanine aminotransferase, gamma glutamyltransferase and alkaline phosphatase was check too. Gynecological exam was performed by rectal palpation and trans rectal ultrasound. At the same time, the uterus was assessing by the size, position, uterine horns consistence and symmetry. It was important to follow uterine and ovary involution observing size and ovarian structures for return of luteal ovarian cycle activity. Moreover, vaginoscopy exam was done to evaluated characteristic and presence of vaginal discharge. The uterine infections were classifying as clinical metritis and puerperal; clinical endometritis according to characteristics and vaginal discharge during time; and subclinical endometritis when diagnosed by endometrial cytology. The Parametric variables was submitted on Turkey test and non-parametric variables on Kruskall-Wallis test. Spearman test was uses to correlation, with 5% significance in all levels. Clinic parameters as cardiac frequency, respiratory frequency and temperature was found normal in pattern of species. Body condition score diversified for 4 to 2,5 and weight for 273 to 315 kg. It was observed that body condition score and weight was low at D-15 compared to other days. For this reason, is important to add strategies that minimize the effects of lost weight during post-partum. The weight observed was different between groups at distinct time. During 60 days post-partum 33,96% of female cows had return to ovarian activity. Ovarian size was not different between groups or moments. Uterus and cervix size decrease between D-15 and other days, however do not observed difference between groups. The incidence of uterine infections was 33,96%, being that 27,78% of cows with infection was primiparous. Dairy cattle that had infection 50% acquired clinical metritis of which 33,33% was primiparous, and 50% acquired clinical endometritis being 22,22% primiparous as well. Endometritis subclinical was not acquired in the groups. The decrease of body condition score was not an influence on occurrence of uterine infections and return of ovarian cyclicity. Correlation between ketosis and uterine infection do not occur, similarly for post-partum ovarian cyclicity return. Changes in metabolic enzymatic liver function was not detected in this research.