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Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus
Mariano, Douglas Oscar Ceolin; Messias, Marcela Di Giacomo; Spencer, Patrick Jack; Pimenta, Daniel Carvalho.
Afiliação
  • Mariano, Douglas Oscar Ceolin; Butantan Institute. Laboratory of Biochemistry and Biophysics. São Paulo. BR
  • Messias, Marcela Di Giacomo; Nuclear and Energy Research Institute. Biotechnology Center. São Paulo. BR
  • Spencer, Patrick Jack; Nuclear and Energy Research Institute. Biotechnology Center. São Paulo. BR
  • Pimenta, Daniel Carvalho; Butantan Institute. Laboratory of Biochemistry and Biophysics. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; 25: e20190029, 2019. tab
Article em En | LILACS, VETINDEX | ID: biblio-1020025
Biblioteca responsável: BR68.1
ABSTRACT
Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins.

Methods:

Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis.

Results:

Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes.

Conclusions:

Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland.(AU)
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Texto completo: 1 Base de dados: LILACS / VETINDEX Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: LILACS / VETINDEX Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Ano de publicação: 2019 Tipo de documento: Article