Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model
Acta cir. bras
; 36(7): e360702, 2021. tab, graf
Article
em En
| LILACS, VETINDEX
| ID: biblio-1284915
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans.
Palavras-chave
Texto completo:
1
Base de dados:
LILACS
/
VETINDEX
Idioma:
En
Revista:
Acta cir. bras
Ano de publicação:
2021
Tipo de documento:
Article