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Comparison of different diagnostic modalities for isolation of Mycobacterium Tuberculosis among suspected tuberculous lymphadenitis patients / Comparação de diferentes modalidades de diagnóstico para isolamento de Mycobacterium tuberculosis entre pacientes com suspeita de linfadenite tuberculosa
Sharif, N; Ahmed, D; Mahmood, R T; Qasim, Z; Khan, S N; Jabbar, A; Khattak, A A; Asad, M J; Ahmed, W; Khan, M M; Awan, U A; Zaman, N; Habiba, U; Noureen, S; Alghamdi, H A.
Afiliação
  • Sharif, N; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Ahmed, D; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Mahmood, R T; Mirpur University of Science and Technology. Department of Biotechnology. Mirpur. PK
  • Qasim, Z; Divisional Headquaters Teaching Hospital. Department of Pathology. Mirpur. PK
  • Khan, S N; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Jabbar, A; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Khattak, A A; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Asad, M J; University Institute of Biochemistry and Biotechnology. PMAS-Arid Agriculture University Rawalpindi. Punjab. PK
  • Ahmed, W; The University of Haripur. Department of Microbiology, Haripur. PK
  • Khan, M M; The University of Haripur. Department of Microbiology, Haripur. PK
  • Awan, U A; The University of Haripur. Department of Medical Laboratory Technology. Haripur. PK
  • Zaman, N; University of Swat. Centre for Biotechnology and Microbiology. PK
  • Habiba, U; The University of Haripur. Department of Forestry and Wildlife Management. Haripur. PK
  • Noureen, S; The University of Haripur. Department of Forestry and Wildlife Management. Haripur. PK
  • Alghamdi, H A; King Khalid University. College of Sciences, Department of Biology. Abha. SA
Braz. j. biol ; 83: e244311, 2023. tab, graf
Article em En | LILACS, VETINDEX | ID: biblio-1285616
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Tuberculosis is a communicable disease with high morbidity and mortality rates in developing countries. The study's primary objective is to compare conventional methods such as acid-fast bacillus (AFB) culture and microscopy with rapid diagnostic methods. The secondary objective is to compare histopathological and microbiological findings in suspected patients with tubercular lymphadenitis. A total of 111 samples (August 2018 to September 2019) of lymph nodes were processed for AFB microscopy, AFB cultures, drug-susceptibility testing (DST), histopathology, and Xpert Mycobacterium Tuberculosis (MTB)/resistance to Rifampin (RIF) assays. Out of 111 lymph node samples, 6 (5.4%) were positive for AFB smear microscopy, 84 (75.6%) were positive for AFB culture, 80 (70.7%) were positive on Gene Xpert, and 102 (91.8%) were indicative of tuberculosis for histopathology studies. Mycobacteria growth indicator tube (MGIT) culture positivity was 84 (75.6%) higher than solid Lowenstein-Jensen (LJ) culture 74 (66.6%). Positive cultures underwent phenotypic DST. Two cases were Multidrug-resistant (MDR) on DST, while three cases were Rifampicin resistant on Gene Xpert. The sensitivity of Genexpert was (62%) against the conventional AFB culture method. The poor performance of conventional lymphadenitis diagnostic methods requires early and accurate diagnostic methodology. Xpert MTB/RIF test can help in the treatment of multidrug-resistant TB cases. Nonetheless, rapid and conventional methods should be used for complete isolation of Mycobacterium tuberculosis.
RESUMO
Resumo A tuberculose é uma doença transmissível com altas taxas de morbimortalidade nos países em desenvolvimento. O objetivo principal do estudo é comparar métodos convencionais, como cultura de bacilo álcool-ácido resistente (BAAR) e microscopia, com métodos de diagnóstico rápido. O objetivo secundário é comparar os achados histopatológicos e microbiológicos em pacientes com suspeita de linfadenite tubercular. Um total de 111 amostras (agosto de 2018 a setembro de 2019) de gânglios linfáticos foi processado ​​para microscopia de AFB, culturas de AFB, teste de susceptibilidade a drogas (DST), histopatologia e Xpert Mycobacterium tuberculosis (MTB)/ensaios de resistência à rifampicina (RIF). Das 111 amostras de linfonodos, 6 (5,4%) foram positivas para baciloscopia de AFB, 84 (75,6%) foram positivas para cultura de AFB, 80 (70,7%) foram positivas para o GeneXpert e 102 (91,8%) foram indicativas de tuberculose para estudos histopatológicos. A positividade da cultura do tubo indicador de crescimento de micobactérias (MGIT) foi 84 (75,6%), maior que a cultura sólida de Lowenstein-Jensen (LJ), 74 (66,6%). As culturas positivas foram submetidas a DST fenotípico. Dois casos eram multirresistentes (MDR) ao DST, enquanto três casos eram resistentes à rifampicina no GeneXpert. A sensibilidade do GeneXpert foi 62% contra o método convencional de cultura AFB. O fraco desempenho dos métodos convencionais de diagnóstico de linfadenite requer metodologia de diagnóstico precoce e precisa. O teste Xpert MTB/RIF pode ajudar no tratamento de casos de tuberculose multirresistente. No entanto, métodos rápidos e convencionais devem ser usados ​​para o isolamento completo do Mycobacterium tuberculosis.
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Texto completo: 1 Base de dados: LILACS / VETINDEX Idioma: En Revista: Braz. j. biol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: LILACS / VETINDEX Idioma: En Revista: Braz. j. biol Ano de publicação: 2023 Tipo de documento: Article