Sperm cryopreservation of Prochilodus brevis using different concentrations of non-permeable cryoprotectants
Torres, Thais Maia; Almeida-Monteiro, Priscila Silva de; Nascimento, Renata Vieira do; Pereira, Vanessa Alves; Ferreira, Yasmim Maia; Lobato, Jessica Sales; Pinheiro, Romulo Roberto Ribeiro; Sales, Yara Silvino; Montenegro, Assis Rubens; Salmito-Vanderley, Carminda Sandra Brito.
Anim. Reprod. (Online);
19(1): e20210083, 2022. tab, graf
Artigo
em Inglês
|
LILACS-Express
| ID: biblio-1360099
Resumo
Abstract The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.
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