ErpY-like protein, a promising antigen to leptospirosis control: characterization of antigenic and immunogenic potential
Padilha, Bárbara Couto Roloff; Simão, Henrique Queiroz; Collares, Thais Farias; Freitas, Stella Buchhorn de; Oliveira, Thaís Larré; Schuch, Rodrigo Andrade; Woloski, Rafael dos Santos; Hartwig, Daiane.
Acta sci., Biol. sci;
44: e61160, mar. 2022. ilus, graf
Artigo
em Inglês
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| ID: biblio-1391256
Resumo
ErpY-like protein (LIC11966) is an antigen from Leptospira spp., which is possibly involved in the infection process and, consequently, can be a promising solution for the development of new diagnostic tests and vaccines. Here, the presence of the erpY-like gene was evaluated in several Leptospira serovars by polymerase chain reaction (PCR), and the ErpY-like recombinant protein was produced and characterized in terms of antigenicity and immunogenicity in vivo. The erpY-like gene was detected by PCR in all Leptospira pathogenic serovars tested (n = 8) and was absent in the saprophytic ones. The rErpY-like protein was recognized by antibodies present in the sera of humans and animals (swine and canine) naturally infected, suggesting ErpY-like expression during natural infection. The rErpY-like protein used to immunize mice with Freund's adjuvant stimulated a mixed Th1/Th2 response, an important protective immunity against leptospirosis.(AU)
Biblioteca responsável:
BR68.1
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