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Characterization of the first two toxins isolated from the venom of the ancient scorpion Tityus (Archaeotityus) mattogrossensis (Borelli, 1901)
Oliveira, Natiela Beatriz de; Magalhães, Ana Carolina Martins; Bloch Junior, Carlos; Beirão, Paulo Sérgio Lacerda; Silva, Anita de Oliveira; Melani, Rafael D; Barbosa, Eder Alves; Pires Júnior, Osmindo Rodrigues; Schwartz, Carlos Alberto.
Afiliação
  • Oliveira, Natiela Beatriz de; University of Brasilia. Institute of Biology. Department of Physiological Sciences. Laboratory of Toxinology. Brasília. BR
  • Magalhães, Ana Carolina Martins; University of Brasilia. Institute of Biology. Department of Physiological Sciences. Laboratory of Toxinology. Brasília. BR
  • Bloch Junior, Carlos; EMPRAPA. Center of Genetic Resources and Biotechnology, Brazilian Agricultural Research Corporation. Brasília. BR
  • Beirão, Paulo Sérgio Lacerda; Federal University of Minas Gerais. Institute of Exact Sciences. Department of Biochemistry and Immunology. Belo Horizonte. BR
  • Silva, Anita de Oliveira; Federal University of Minas Gerais. Institute of Exact Sciences. Department of Biochemistry and Immunology. Belo Horizonte. BR
  • Melani, Rafael D; University of Brasilia. Institute of Biology. Department of Physiological Sciences. Laboratory of Toxinology. Brasília. BR
  • Barbosa, Eder Alves; University of Brasilia. Institute of Chemistry. Laboratory of Biomolecule Synthesis and Analysis. Brasília. BR
  • Pires Júnior, Osmindo Rodrigues; University of Brasilia. Institute of Biology. Department of Physiological Sciences. Laboratory of Toxinology. Brasília. BR
  • Schwartz, Carlos Alberto; University of Brasilia. Institute of Biology. Department of Physiological Sciences. Laboratory of Toxinology. Brasília. BR
J. venom. anim. toxins incl. trop. dis ; 27: e20210035, 2021. graf, tab
Article em En | VETINDEX | ID: biblio-1395711
Biblioteca responsável: BR68.1
ABSTRACT

Background:

Almost all Tityus characterized toxins are from subgenera Atreus and Tityus, there are only a few data about toxins produced by Archaeotityus, an ancient group in Tityus genus.

Methods:

Tityus (Archaeotityus) mattogrossensis crude venom was fractionated by high performance liquid chromatography, the major fractions were tested in a frog sciatic nerve single sucrose-gap technique. Two fractions (Tm1 and Tm2) were isolated, partially sequenced by MALDI-TOF/MS and electrophysiological assayed on HEK293 Nav 1.3, HEK293 Nav 1.6, DUM and DRG cells.

Results:

The sucrose-gap technique showed neurotoxicity in four fractions. One fraction caused a delay of action potential repolarization and other three caused a reduction in amplitude. An electrophysiological assay showed that Tm1 is active on HEK293 Nav 1.3, HEK293 Nav 1.6, DUM and DRG cells, and Tm2 on HEK293 Nav 1.3 and DRG cells, but not in HEK293 Nav 1.6. In addition, Tm1 and Tm2 did promote a shift to more negative potentials strongly suggesting that both are α-NaScTx.

Conclusion:

Although Tityus (Archaeotityus) mattogrossensis is considered an ancient group in Tityus genus, the primary structure of Tm1 and Tm2 is more related to Tityus subgenus. The patch clamp electrophysiological tests suggest that Tm1 and Tm2 are NaScTx, and also promoted no shift to more negative potentials, strongly suggesting that both are α-NaScTx. This paper aimed to explore and characterize for the first time toxins from the ancient scorpion Tityus (Archaeotityus) mattogrossensis.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Ano de publicação: 2021 Tipo de documento: Article