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Effect of different concentrations of Trolox® in association with docosahexaenoic acid on equine semen freezing

Aguiar, Cristiane Silva; Barros, Celso Henrique Souza Costa; Machado, William Morais; Allaman, Ivan Bezerra; Leite Filho, Antônio de Oliveira; Barbosa, Larissa Pires; Snoeck, Paola Pereira das Neves.
Anim. Reprod. (Online); 19(4): e20220010, 2022. tab
Artigo em Inglês | VETINDEX | ID: biblio-1403212

Resumo

The aim of this study was to evaluate the association of different concentrations of Trolox® and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio® (BC; Control); E2) BC + 50 ngml-1 DHA + 30 µM Trolox® (BCDHA30T); E3) BC + 50 ngml-1 DHA + 40 µM Trolox® (BCDHA40T); E4) BC + 50 ngml-1 DHA + 50 µM Trolox® (BCDHA50T). All the tested extenders were similar in preserving different kinematic parameters, cell functional integrity, compacted DNA, and high and intermediate mitochondrial activity (P>0.05). However, sperm cryopreserved in BCDHA40T showed higher velocities than sperm frozen in the control extender (P<0.05). The 30 µM concentration of Trolox® was worse for sperm motility and the 50 µM concentration of Trolox® did not adequately preserve the structural integrity of the membranes in an extender containing DHA when compared to the BotuCrio® (P<0.05) extender. The use of Trolox® in freezing extenders containing DHA did not maximize the effect of BotuCrio®, except for in the case of sperm velocity parameters when at a concentration of 40 µM.(AU)
Biblioteca responsável: BR68.1