Your browser doesn't support javascript.
loading
Action of swim-up and caffeine on equine frozen sperm
Alves, Natália de Castro; Diniz, Soraia de Araújo; Viegas, Rodrigo Novaes; Arigoni, Ana Luiza; Freitas, Marina Morra; Lana, Ângela Quintão; Lagares, Monique de Albuquerque.
Afiliação
  • Alves, Natália de Castro; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Diniz, Soraia de Araújo; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Viegas, Rodrigo Novaes; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Arigoni, Ana Luiza; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Freitas, Marina Morra; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Lana, Ângela Quintão; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
  • Lagares, Monique de Albuquerque; Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte. BR
Anim. Reprod. (Online) ; 19(4): e20220056, 2022. tab, ilus
Article em En | VETINDEX | ID: biblio-1403213
Biblioteca responsável: BR68.1
ABSTRACT
Cryopreservation of equine semen is crucial to semen commercialization. However, it reduces sperm motility and longevity. Thus, sperm selection methods and addition of motility-activating substances to sperm, such as caffeine, may improve sperm quality of equine frozen semen. The objective of the current work was to evaluate the effects of caffeine on recovery and quality parameters of frozen-thawed sperm subjected to swim-up selection to be used in intracytoplasmic sperm injection (ICSI) in assisted reproductive techniques. Stallion semen were frozen and after thawing different caffeine concentrations were added to the samples performing four treatments control (no caffeine), 3, 5, and 7.5 mM caffeine. Sperm kinematic and motility were assessed by computer-assisted sperm analysis (CASA). Then, the four treated samples were submitted to the swim-up sperm selection, and the number of recovered sperm and morphology were evaluated at four times 20, 40, 60, and 80 min. The swim-up increased the recovery proportion of normal morphology sperm without (80.1±1%) or with caffeine addition (3mM 81.2±1%, 5mM 79.9±1% and 7.5 mM 78.9±1%) compared to the thawed semen (70±2%). However, the addition of 5 mM caffeine induced an increase in sperm motility (38.9±2.8 vs. 32.6±3.4%, P<0.05), and sperm recovery after swim-up (7.9x106 vs. 3.4x106 sperm/ml, P<0.05) compared to the control. The addition of 5 mM caffeine to frozen-thawed equine semen before swim-up selection improved sperm motility and increased the sperm recovery rate while not decreasing the percentage of morphologically normal sperm. Thus, caffeine addition to frozen-thawed equine semen before swim-up selection has potential clinical application in improving sperm quality for use in ICSI.(AU)
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2022 Tipo de documento: Article