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Activated charcoal in the control of oxidation and in vitro growth of Dioscorea spp. nodal segments

Verde, Denise dos Santos Vila; Mendes, Maria Inês de Souza; Souza, Antônio da Silva; Rabêlo, Jéssica Sales; Rabêlo, Jéssica Sales; Nobre, Leila Vasconcelos Costa; Ledo, Carlos Alberto da Silva.
Acta sci., Biol. sci; 44: e61950, mar. 2022. tab
Artigo em Inglês | VETINDEX | ID: biblio-1413340

Resumo

In vitrooxidationisaproblem forsome herbaceous and woody species and can causedarkening of tissues and consequently death of explantsand plants Therefore, this study aimed to assess the effect of activated charcoal on in vitro yamcultivation, aiming at reducing or eliminating explant oxidation and optimizing the growth of the genotypes Dioscorea alata var.purpurea(Roxb.) A. Pouchet and Dioscorea rotundataPoir. Nodal segments of approximately 1 cm, extracted from plants previously grown in vitro, were introduced into test tubes containing 10 mL of 2GGC culture medium, plus 30 g L-1sucrose, solidified with 2.2 g L-1Phytagel®and pH adjusted to 5.8 before autoclaving, containing activated charcoal doses of 0, 1, 2, 3 and 4 g L-1. Plants were maintained for 90 days in a growth room, with temperature of 27 ± 1ºC, photon flux density of 30 µmol m-2s-1and photoperiod of 16 hours, after which their development variables were evaluated. Activated charcoal, at the concentration of 4 g L-1considerably promoted the best development of plants, and the species D. alatavar.purpureashowed higher means for all variables studied.(AU)
Biblioteca responsável: BR68.1