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A modified hydrogel production protocol to decrease cellular content
Braga, Gabriela Catão Diniz; Camargo, Cristina Pires; Harmsen, Martin Conrad; Correia, Aristides Tadeu; Souza, Sonia; Seelaender, Marilia; Nunes, Viviane Araujo; Santos, Jeniffer Farias dos; Neri, Elida Adalgisa; Valadão, Iuri Cordeiro; Moreira, Luiz Felipe Pinho; Gemperli, Rolf.
Afiliação
  • Braga, Gabriela Catão Diniz; s.af
  • Camargo, Cristina Pires; s.af
  • Harmsen, Martin Conrad; University Medical Center Groningen. NL
  • Correia, Aristides Tadeu; s.af
  • Souza, Sonia; s.af
  • Seelaender, Marilia; Universidade de São Paulo. São Paulo. BR
  • Nunes, Viviane Araujo; Universidade de São Paulo. São Paulo. BR
  • Santos, Jeniffer Farias dos; s.af
  • Neri, Elida Adalgisa; s.af
  • Valadão, Iuri Cordeiro; s.af
  • Moreira, Luiz Felipe Pinho; Universidade de São Paulo. São Paulo. BR
  • Gemperli, Rolf; Universidade de São Paulo. São Paulo. BR
Acta cir. bras ; 37(10): e371005, 2022. ilus, graf
Article em En | VETINDEX | ID: biblio-1415435
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.

Methods:

We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.

Results:

After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.

Conclusions:

The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta cir. bras Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Acta cir. bras Ano de publicação: 2022 Tipo de documento: Article