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In vivo embryo development in bitches inseminated laparoscopically after ovulation time estimated based on a single progesterone determination
Alves, Aracelle Elisane; Motheo, Tathiana Ferguson; Apparicio, Maricy Ferreira; Mostachio, Giuliano Queiroz; Santos, Ricarda Maria dos; Vicente, Wilter Ricardo Russiano; Luvoni, Gaia Cecilia.
Afiliação
  • Alves, Aracelle Elisane; Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Departamento de Cirurgia Veterinária. Uberlândia. BR
  • Motheo, Tathiana Ferguson; Universidade de Cuiabá. Cuiabá. BR
  • Apparicio, Maricy Ferreira; Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Reprodução Animal. Botucatu. BR
  • Mostachio, Giuliano Queiroz; Centro Universitário de Rio Preto. Faculdade de Medicina Veterinária. Departamento de Cirurgia Veterinária. Rio Preto. BR
  • Santos, Ricarda Maria dos; Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Departamento de Cirurgia Veterinária. Uberlândia. BR
  • Vicente, Wilter Ricardo Russiano; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Reprodução Animal. Jaboticabal. BR
  • Luvoni, Gaia Cecilia; Università Degli Studi di Milano. Dipartimento di Medicina Veterinaria e Scienze Animali. Milano. IT
Anim. Reprod. (Online) ; 20(1): e20220079, 2023. tab
Article em En | VETINDEX | ID: biblio-1418572
Biblioteca responsável: BR68.1
ABSTRACT
Logistic and economical limitations are often the causes of dog owners not accurately monitoring the estrous cycle and the optimal insemination time. The aim of this study was to evaluate in vivo early embryonic development in bitches, after the analysis of sequential vaginal cytologies associated to single progesterone measurement and single laparoscopic insemination with high quality semen (fresh and with high spermatozoa concentration) or low-quality semen (frozen/thawed and with low spermatozoa concentration) at 48 h post- ovulation time predicted on a single progesterone measurement. Ten bitches were inseminated with 250 x 106 fresh spermatozoa (80% motility), and ten with 80 x 106 frozen/thawed spermatozoa (60% motility) in the cranial part of each uterine horn. Seven days later, ovariohysterectomy was performed and the oviducts and uterine horns and body were flushed to recover embryos and unfertilized oocytes. In 80% of the bitches inseminated with fresh and 50% of bitches inseminated with frozen/thawed semen, embryos at 2 to 8 cells stage were recovered mostly from the, oviducts. This study indicates that pregnancies can be obtained with a single laparoscopic intrauterine insemination after single serum progesterone measurement, although with a low number of embryos. This result should be taken into account in case economic or logistic restrictions that affect the possibility of owners to plan an accurate monitoring of the optimal breeding time using fresh and frozen semen.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2023 Tipo de documento: Article