Activity of the decoction of Achyrocline satureioides D.C. (Lam.) - Asteraceae (macela) against standard and isolated bacteria from bovine mastitis / Atividade do decocto de Achyrocline satureioides D.C. (Lam.) - Asteraceae (macela) sobre bactérias padrões e isoladas em mastite bovina

ABSTRACT

Background: Mastitis is economically considered the most important disease in dairy cattle. Reports on the increase of organisms that are resistant to antimicrobial drugs, as well as on the demand for sanitary inputs which can be used in organic and agroecological animal production models motivate the research and discovery of new vegetal-based drugs for their control. The objective of this study was to evaluate the antibacterial activity of the galenic form decoction of Achyrocline satureioides (Lam.) D.C. - Asteracea (macela) against standard and isolated bacteria found in bovine mastitis.Materials, Methods & Results: A. satureioides was collected from a single site and its exsiccate was identifi ed and registered at the Instituto de Biociências of the UFRGS under number ICN 161.026. The decoction was obtained from the cooking of infl orescences with distilled water, with subsequent replacement of the evaporated volume. The solution was analyzed by thin layer chromatography (TLC) and the phytochemical quality was verifi ed based on the presence of a fl avonoid, quercetine. The antibacterial activity was evaluated using the pour plate technique, leaving the decoction in contact with the bacteria for 1 h and 24 h. After the pre-established contact times, the plates received ± 30 mL of a solid Mueller-Hinton culture medium without (reading of the bacterial inhibition indic

RESUMO

Background: Mastitis is economically considered the most important disease in dairy cattle. Reports on the increase of organisms that are resistant to antimicrobial drugs, as well as on the demand for sanitary inputs which can be used in organic and agroecological animal production models motivate the research and discovery of new vegetal-based drugs for their control. The objective of this study was to evaluate the antibacterial activity of the galenic form decoction of Achyrocline satureioides (Lam.) D.C. - Asteracea (macela) against standard and isolated bacteria found in bovine mastitis.Materials, Methods & Results: A. satureioides was collected from a single site and its exsiccate was identifi ed and registered at the Instituto de Biociências of the UFRGS under number ICN 161.026. The decoction was obtained from the cooking of infl orescences with distilled water, with subsequent replacement of the evaporated volume. The solution was analyzed by thin layer chromatography (TLC) and the phytochemical quality was verifi ed based on the presence of a fl avonoid, quercetine. The antibacterial activity was evaluated using the pour plate technique, leaving the decoction in contact with the bacteria for 1 h and 24 h. After the pre-established contact times, the plates received ± 30 mL of a solid Mueller-Hinton culture medium without (reading of the bacterial inhibition indic