A clinical trial and oral wound treated by essential oil of Lippia sidoides mouthrinse in horses

ABSTRACT

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...