Isolation, culture and differentiation potential of collared peccary (Tayassu tajacu) adipose-derived stem cells

ABSTRACT

Background: The understanding of cell biology and the isolation of mesenchymal stem cells in wild animals show prospects for conducting pre-clinical trials in these unconventional animals. The collared peccary (Tayassu tajacu) are suiforms that belong to the Artiodáctyla order, Tayassuidae family and Tayassu genus. They adapt easily to captivity conditions that favors their commercial rearing and is an alternative for biodiversity conservation. To evaluate the collared peccary (Tayassu tajacu) as a potential animal model for the isolation of mesenchymal progenitor cells, cell culture and cell differentiation protocols. Materials, Methods & Results: To perform this research we used four collared peccaries (Tayassu tajacu) from the Nucleus of Study and Preservation of Wild Animals (IBAMA/PI No . 02/08-618) from Federal University of Piauí (UFPI). Adipose tissue fragments were collected from the dorsocervical region and dissociated mechanically in laboratory. The material was placed in an incubator containing CO2 - 95% at 37C and the cultures were expanded to fifth passage, evalluating cell concentration and viability. The culture medium alfa-MEM supplemented was changed every three days. The cell kinetics was evaluated in triplicate using growth curve performed during ten days, plating the initial concentration of 5 x 104 cells/mL per well in P3 six-well culture plate...