Técnica do cometa para avaliação da integridade do DNA de espermatozoides caprinos criopreservados em ACP-101/102® / Comet technique to assess the DNA integrity of cryopreserved goat sperm in ACP-101/102®

ABSTRACT

Animal reproduction represents one of the most important factors, and the use of reproductive biotechnologies that help increase production is essential. The comet technique is the simple and quick way to detect pre-mutagenic lesions and assist in studies on environmental biomonitoring, toxicological genetics, biological radiation, DNA repair process and genetic ecotoxicology. The objective of this study was to evaluate the viability of DNA from sperm cells from goat semen submitted to the cryopreservation process in powdered coconut water (ACP101c/102c). The experiment was carried out at the Federal University of Piauí with two goats of reproductive age. The evaluation of the spermatic DNA integrity was performed using an immunofluorescence microscope. Classes from 0 to 3 were used, 0 being no damage and 3, the tail of the comet greater than twice the size of the nucleoid. The results obtained in the G2 test group (ACP 101c-102c) showed a slight tail formation, indicating a slight fragmentation of DNA. It was concluded that in the control group GC1 (TRIS + egg yolk of Gallu gallus domesticus) and experimental group (ACP 101c - 102c) there was no significant difference.